Objective To establish a paper spray mass spectrometry method for the determination of 1-methoxy-2-propanol and 2-methoxypropionic acid as biomarkers of propylene glycol monomethyl ether in urine. Methods The urine sample was dropped onto the chromatographic paper, and a high voltage of 4 kV was applied to the back of the paper. After dissolution in the spray solvent, the sample formed an electrospray at the paper tip under the effect of voltage, which then entered the mass spectrometer for detection. Results The linear ranges of 1-methoxy-2-propanol and 2-methoxypropionic acid were 0.036-46.15 μg/mL and 0.029-54.25 μg/mL, respectively, with correlation coefficients ranging from 0.999 6 to 0.999 9. The detection limit (LOD) was between 0.008 8 and 0.011 μg/mL, and the quantification limit(LOQ) was between 0.029 and 0.036 μg/mL. The sample recovery rate ranged from 90.8% to 105.0%, and RSDs ranged from 3.3% to 9.7%. Conclusion This method requires no sample processing, features simplicity, rapidity and high sensitivity, making it suitable for the rapid screening of propylene glycol monomethyl ether in urine.

Objective To investigate the effect of aerobic exercise on blood pressure in patients with resistant hypertension through meta-analysis, aiming to identify more effective intervention strategies and provide reference for the control and treatment of resistant hypertension. Methods A systematic search was performed in CBM, CNKI, WANFANG, VIP, PubMed, OVID,Embase, and Web of Science for randomized controlled trials (RCTs) published in Chinese or English from inception to March 25, 2025. Two researchers independently conducted study selection and data extraction. Risk of bias was assessed using RoB 2,and statistical analyses were performed with RevMan 5.4. Results A total of 10 studies were included, comprising 532 cases.Meta-analysis showed that aerobic exercise significantly reduced systolic blood pressure (SBP) (SMD=0.77, 95% CI: 0.57-0.96, P<0.000 01), diastolic blood pressure (DBP) (SMD=0.83, 95% CI: 0.63-1.02, P<0.000 01), 24-hour SBP (SMD=0.48,95% CI: 0.23-0.73, P=0.000 2), and 24-hour DBP (SMD=0.43, 95% CI: 0.17-0.68, P=0.000 9). Subgroup analyses indicated greater SBP reductions among participants aged≤60 years, with BMI≤28 kg/m², exercise intensity >60% VO_2max, and intervention>12 weeks. DBP improvements were more evident in subgroups with age≤60 years, BMI≤28 kg/m², intensity ≤60% VO_2max, and duration >12 weeks. For 24-hour SBP, larger effects were found in subgroups with age>60 years, BMI≤28 kg/m², intensity≤60%VO_2max, and duration >12 weeks. In contrast, greater 24-hour DBP reductions occurred in participants aged≤60 years, with BMI≤28 kg/m², intensity >60% VO_2max, and duration≤12 weeks. Conclusion Aerobic exercise has a positive effect on reducing blood pressure in patients with resistant hypertension. Differences in participant characteristics and intervention protocols may affect the extent of this effect. The current relevant studies are limited in number and most have some risk of bias. However, the limited quantity and quality of existing studies highlight the need for further high-quality research to confirm and extend these findings.

Objective Based on the Python platform, the entire process of vector-borne infectious disease mathematical models is expanded to better fit models and evaluate intervention effects, to provide new ideas for grassroots prevention and control, and to open up new perspectives. Methods The SmEmIm-SpEpIpApRp model was fitted using the lmfit library, solved with the solve_ivp function, and sensitivity analysis of key model parameters was performed. The Rt calculation was based on the next generation matrix method, and all results were visually displayed with the help of Matplotlib. Results The results showed that R²=0.98 and RMSE=2.07. Rt was 5.607 in the early stage of the epidemic, and Rt_peak was 8.439 on day 41. The period with Rt>1 lasted 81 days, and q had the highest sensitivity (S=35.435). Under a single intervention, when β_mp and β_pm<0.01, Rt<1 and the epidemic disappeared. Controlling only γ and q would not eliminate the epidemic. Under comprehensive intervention, Scenario 1 could reduce the cumulative cases by 98.64%, and Rt_peak=0.868. Scenario 2 could reduce the cumulative cases by 87.95%,and Rt_peak=1.988. For Scenario 3, Rt_peak=4.78. Although the increase in Rt was smaller and the change rate was low, the longer duration could increase cumulative cases by 161.47%. Scenario 4 could reduce the cumulative cases by 99.38%, and Rt_peak=0.28. Conclusions The high goodness of fit of the model based on the Python platform verifies the necessity of seasonal dynamic modeling, provides an integrated solution for the prevention and control of vector-borne infectious diseases, expands the practical boundaries of theoretical models, and opens a new perspective for precise prevention and control at the grassroots level.

Vaccination is one of the most effective measures for preventing infectious diseases. However, in recent years, vaccine hesitancy has become increasingly prominent, and the World Health Organization has identified it as one of the “top ten threats to global health” in 2019. Some developed countries have formulated targeted interventions by identifying the influencing factors of vaccine hesitancy. In contrast, systematic research on vaccine hesitancy in China remains limited.Therefore, this review aims to summarize the influencing factors of vaccine hesitancy reported both domestically and internationally, in order to provide a theoretical foundation for developing intervention strategies in China.

Objective To empirically study the core competences evaluation index system for the doctor of public health (DrPH) in China, and to test its discriminant ability, reliability and validity. Methods This study designed the corresponding questionnaire in March 2025. The stratified cluster sampling method was used to select 200 doctors of public health who were enrolled from 2021 to 2024 to conduct a questionnaire survey in China. Cronbach’s α coefficient was used to assess reliability.Pearson correlation analysis assessed internal consistency. T test and F test assessed discriminant validity. Exploratory factor analysis assessed structural validity. Results A total of 189 questionnaires were retrieved. There were 189 valid questionnaires.The effective rate of questionnaire was 100%. The overall Cronbach’s α coefficient of the ability evaluation index system was 0.969, the Cronbach’s α coefficient of the basic knowledge dimension was 0.691, and the Cronbach’s α coefficient of other dimensions were above 0.8. The correlation coefficient between each dimension and the total score was between 0.5 and 0.9.There were statistically significant differences in core competence scores of public health doctors in different grades, public health practitioner certificates, public health practice experience and parents’ occupations (P<0.05). KMO value was 0.916,Bartlett’s spherical test value P<0.001, six common factors were extracted, and the total contribution rate of factor variance was 69.9%. Common factors could basically reflect the core concepts and main dimensions of the evaluation index system for the core competence of doctors in public health. Conclusion The core competences evaluation index system for the DrPH demonstrates good reliability and validity, indicating that it can serve as a tool for the training, evaluation, and assessment of the core competences for the DrPH.

Objective To examine the relationship between body roundness index (BRI) and depression risk in individuals with hyperlipidemia. Methods Data were obtained from the National Health and Nutrition Examination Survey (NHANES) 2005—2018, including 20 492 adults with hyperlipidemia. Multivariable logistic regression and restricted cubic spline analyses were used to analyze the association between BRI and depressive status. Results Multivariable analysis revealed a significant U-shaped relationship between BRI and depression risk (P=0.002), with an inflection point at 3.15. For BRI<3.15, each unit increase in BRI was associated with a 33% decrease in depression risk (OR=0.67, 95% CI: 0.50-0.88, P=0.004 3). For BRI≥3.15, each unit increase in BRI was associated with a 7% increase in depression risk (OR=1.07, 95% CI: 1.04-1.09, P<0.000 1). In tertile analysis, the highest BRI tertile showed a 30% increased risk of depression compared to the lowest tertile(OR=1.30, 95% CI: 1.13-1.49, P=0.000 2). Conclusion There is a U-shaped relationship between BRI and depression risk in individuals with hyperlipidemia, suggesting that both excessively low and high BRI values may increase the risk of depression.Large prospective cohort studies are needed to validate these findings.

Objective To establish a prediction model to assess the risk of death in patients with Klebsiella pneumoniae (Kp) infection, and to provide a reference for the development of targeted prevention and control strategies for nosocomial infections. Methods Patients with Klebsiella pneumoniae(Kp) infection at the Affiliated Hospital of Guizhou Medical University from January 2020 to December 2022 were used as the training dataset. LASSO-logistic regression was applied to identify independent risk factors for death in Kp-infected patients, and a mortality risk nomogram was constructed. Patients with Kp infection from January 2023 to December 2023 were used as the validation dataset. Model discrimination, accuracy, and clinical utility were comprehensively evaluated using the area under the receiver operating characteristic curve (ROC), the calibration curve, and the decision analysis curve. Results A total of 1 972 Kp-infected patients were included in the training dataset, of which 234 Kp-infected patients died with a mortality rate of 11.87%. A total of 1 148 Kp-infected patients were included in the validation dataset, of which 126 Kp-infected patients died with a mortality rate of 10.98%. The results of LASSO-logistic regression modeling showed that patients aged >70 years, carbapenem resistance, mechanical ventilation, indwelling urinary catheter, diabetes mellitus, hematologic infections, white blood cell count >10×10⁹/L and hemoglobin <115 g/L were all associated factors for death in patients with Kp infection (OR=24.6, 95% CI: 16.56-39.38; OR=2.44, 95% CI: 1.70-3.52; OR=4.97, 95% CI: 3.08-8.21; OR=2.99, 95% CI: 1.75-5.19; OR=18.24, 95% CI: 9.27-38.12; OR=2.75, 95% CI: 1.69-4.49; OR=4.18, 95% CI: 2.88-6.09; OR=2.65, 95% CI: 1.79-3.93). The areas under the ROC curve of the training dataset and validation dataset were 0.900 (95% CI: 0.876-0.924) and 0.843 (95% CI: 0.812-0.874), respectively. The calibration curve was close to the baseline curve, and the decision analysis curve was close to the upper right corner. Conclusion By analyzing the factors related to the death of Kp-infected patients, we constructed a nomogram prediction model of the risk of death of Kp-infected patients, which has good accuracy, discrimination, and clinical utility, and according to which targeted nosocomial infection prevention and control measures can be implemented.

Objective This study aims to explore the relationship between socioeconomic status (SES) and intrinsic capacity (IC) in older adults, as well as the mediating role of healthy lifestyle. Methods Based on data from the Hubei Memory and Aging Cohort Study (HMACS), SES was assessed based on education, occupation, and income, and then categorized into high, medium, and low groups. Healthy lifestyle score (0-6 points) was constructed based on smoking, drinking, diet, social activities, physical exercise, and intellectual leisure activities. IC was assessed according to the indicators recommended by the WHO (2015). Group differences were compared using χ² tests or variance analysis. We performed a multivariable logistic regression analysis to assess the association between SES and IC impairment, and general linear regression models were used to analyze the association between SES and IC. A mediation model was employed to assess the mediating effect of healthy lifestyles on the association between SES and IC, with comparisons across sex and SES groups. Results A total of 5 628 eligible participants aged ≥60 years were included. Older adults with high SES had significantly higher healthy lifestyle scores than those with low SES (P<0.05). The IC impairment in the middle and low SES groups was 1.507 times (95% CI: 1.289-1.762) and 6.476 times (95% CI: 5.051-8.304) than that of the high SES group, respectively, both with P<0.001. SES and IC were significantly positively correlated (β=0.530, 95% CI: 0.505-0.555, P<0.001), with the strongest association in the low SES group (β=1.004,95% CI: 0.773-1.234, P<0.001). Healthy lifestyle partially mediated the relationship between SES and IC, with a mediation effect of 9.25%. The mediation effects were 8.32% in males and 10.19% in females. The mediation effect was significant only in the medium SES group (15.03%), with no significant mediation observed in the low or high SES groups. Conclusion In older adults, SES and IC are significantly positively correlated. SES is associated with IC both directly and indirectly through a healthy lifestyle, with mediating effects varying by sex and SES. Therefore, health promotion measures for older adults should fully consider sex and SES differences, emphasize the improvement of healthy lifestyles, enhance IC in older adults, and promote healthy aging.

Objective To observe the effects of oxygen enrichment on blood oxygen saturation and heart rate in plateau migrants. Methods Oxygen-enriched rooms were created at altitudes of 4 200 m and 5 100 m using different enclosed spaces, maintaining oxygen volume percentages at approximately 25% and 27%, respectively. Changes in blood oxygen saturation and heart rate at different time points before and after oxygen enrichment were compared among subjects. Results Achieving 25%and 27% oxygen concentrations in various enclosed spaces at 4 200 m and 5 100 m required total oxygen volumes of 200-3 840 L, with average oxygen consumption of 32-88.7 L/m³, and maintenance oxygen flow rates of 2-18 L/min. Compared with baseline levels, subjects exhibited significantly increased blood oxygen saturation (P<0.05 or P<0.01) and significantly decreased heart rate (P<0.05 or P<0.01) after oxygen enrichment. Conclusion At 4 200 m and 5 100 m altitudes, increasing oxygen concentrations to 25% and 27%, respectively, can elevate blood oxygen saturation levels and effectively reduce heart rate in subjects. For constructing high-altitude oxygen-enriched rooms, smaller and well-sealed spaces are recommended.

Objective To analyze the changes in the disease burden of stroke attributable to low physical activity in China from 1990 to 2021, and to provide a scientific basis for the development of prevention and control strategies for stroke. Methods Data on mortality, years of life lost (YLLs), years lived with disability (YLDs), and disability-adjusted life years (DALYs) for stroke attributable to low physical activity in China from 1990 to 2021 were extracted from the Global Burden of Disease Study 2021. The disease burden of stroke attributable to low physical activity in China was analyzed by gender and age group from 1990 to 2021. The Joinpoint regression model was utilized to analyze the trends in disease burden. Results From 1990 to 2021, the number of deaths, mortality rates, YLLs, YLLs rates,YLDs, YLDs rates, DALYs, and DALYs rates attributable to low physical activity in China showed an upward trend, with increases of 129.80%, 90.48%, 97.39%, 63.26%, 216.74%, 162.17%, 112.94%, and 76.05%, respectively. The age-standardized mortality rate, age-standardized YLLs rate, and age-standardized DALYs rate showed a downward trend, with decreases of 20.14%, 25.68%, and 18.75%,respectively, while the age-standardized YLDs rate has increased by 18.26% compared to 1990. Joinpoint regression analysis results indicated that the average annual percent change (AAPC) for the age-standardized mortality rate, age-standardized YLLs rate, age-standardized DALYs rate, and age-standardized YLDs rate attributable to low physical activity from 1990 to 2021 were-0.72%, -0.96%, -0.68%, and 0.92%, respectively. Gender-stratified analysis revealed that in 2021, the age-standardized mortality rate, age-standardized YLLs rate, and age-standardized DALYs rate attributable to low physical activity were higher in males than in females. Meanwhile, the decline in the age-standardized mortality rate, age-standardized YLLs rate, and age-standardized DALYs rate attributable to low physical activity was more rapid in females compared to males. Age-stratified results indicated that the disease burden of stroke attributable to low physical activity increased with age but showed a trend toward affecting younger individuals. Conclusion The disease burden of stroke attributable to low physical activity in China remains substantial, indicating a need for targeted and comprehensive prevention and control measures, particularly for key populations such as the elderly, males, and middle-aged and young adults.