To explore the longitudinal correlation between cognitive function (CF) and activities of daily living (ADL) of the rural elderly in Guizhou province, and to provide theoretical basis for preventing and controlling health problems of the elderly.

The multi-stage cluster random sampling method was used to select the elderly aged 60 years and above from 12 villages in 2 counties(districts) of Guizhou Province as the research objects. In this study, 792 elderly people who participated in two surveys in 2019 (T₁) and 2022 (T₂) and were ≥60 years old at baseline with complete variable values were selected as survey subjects. The cross-lagged model was used to investigate the longitudinal relationship between cognitive function and ADL.

The cross-lagged model showed that T₁ ADL could predict T₂ cognitive function after controlling T₁ cognitive function (β= -0.120, P<0.001). After controlling for T₁ ADL, T₁ cognitive function predicted T₂ ADL (β= -0.099, P=0.005). In the study of the association between two dimensions of ADL and cognitive function, it was found that T₁ IADL could predict T₂ cognitive function after controlling for T₁ cognitive function (β= -0.138, P<0.001). Similarly, after controlling T₁ IADL, T₁ cognitive function could predict T₂ IADL (β= -0.124, P<0.001). But there was no predictive effect between BADL and cognitive function.

There is a longitudinal bidirectional correlation between cognitive function and activities of daily living of the rural elderly in Guizhou, only between IADL and cognitive function, suggesting that strengthening the monitoring and intervention of cognitive function of the elderly can help reducing the risk of the decline of IADL in the elderly, and strengthening the training of IADL plays an important role in delaying cognitive decline.

To analyze the burden of diabetes attributable to different risk categories and the trend of diabetes in China from 1990 to 2021, providing references for scientific prevention and treatment of diabetes.

Based on the global burden of disease data in 2021, we used Joinpoint regression to analyze the trends of diabetes deaths, disability adjusted life years (DALYs), and age-standardized mortality rate (ASMR) attributable to metabolic, behavioral, and environmental/occupational risks in China.

In 2021, the number of diabetes deaths attributable to metabolic, behavioral, and environmental/occupational risks in China increased to 178 400, 73 000, 45 900, and the ASMR decreased by 8.65%, 2.41%, and 8.37%, compared to 1990. The number of diabetes DALYs attributable to different risk categories were metabolic, behavioral, and environmental/occupational risks in descending order, and the rate of DALYs and ASDR showed an increasing trend.

The task of reducing the burden of diabetes disease in China remains daunting, and to intensify behavioral risks interventions for men and young people with diabetes.

To analyze whether there is a potential causal association between inflammatory cytokines and various tendinopathies, including Achilles tendonitis, bicipital tendinitis, calcific tendinitis of the shoulder, gluteal tendinitis, and patellar tendinitis.

In this study, single nucleotide polymorphisms from the data of a large-scale genome-wide association study were selected as instrumental variables, and bidirectional Mendelian randomization as well as multivariate Mendelian randomization, were used to investigate the causal effects of inflammatory cytokines and the risk of developing Achilles tendonitis, Bicipital tendinitis, Calcific tendinitis of shoulder, Gluteal tendinitis and Patellar tendinitis.

In forward Mendelian analyses, monocyte chemotactic protein-3（OR=0.896,95% CI:0.806-0.995） and vascular endothelial growth factor（OR=0.917,95% CI:0.845-0.995） were potential protective factors for Achilles tendonitis, and monokine induced by interferon-γ（OR=1.158,95% CI:1.029-1.302） was a potential risk factor for Achilles tendonitis. Monocyte chemotactic protein-3（OR=1.214,95% CI:1.034-1.426）was a potential risk factor for Bicipital tendinitis, while hepatocyte growth factor （OR=0.661,95% CI:0.489-0.893） as a potential protective factor for Bicipital tendinitis. Hepatocyte growth factor （OR=0.730,95% CI:0.557-0.957）was a potential protective factor for Calcific tendinitis of shoulder. Interleukin-13 （OR=0.828,95% CI:0.714-0.960） was a potential protective factor for Gluteal tendinitis. Interleukin-8 （OR=0.649,95% CI:0.444-0.949） as a Patellar tendinitis potential protective factor and tumor necrosis factor-α （OR=1.707,95% CI:1.085-2.685）was a potential risk factor for Patellar tendinitis. In multivariate Mendelian analysis, hepatocyte growth factor（OR=0.687,95% CI:0.511-0.924） remained a protective factor for Bicipital tendinitis. Sensitivity analyses showed that the main analyses were robust without outliers, with no significant outliers, heterogeneity, or horizontal pleiotropy.

The study suggests a potential causal relationship between inflammatory cytokines and the five tendon diseases investigated.

To understand the molecular epidemiological characteristics of multi-drug-resistant (MDR) Salmonella clinical isolates in Guizhou Province and clarify the carrying of β-lactamase resistance genes to provide a reference basis for the prevention and treatment of MDR Salmonella in Guizhou province.

A total of 70 MDR Salmonella were used for the study, antibiotic susceptibility analyses of Salmonella were carried out using the micro broth dilution method, molecular epidemiological analyses were carried out using the method of multilocus sequence typing (MLST). Polymerase Chain Reaction was used to detect the β-lactamase resistance genes in the MDR Salmonella strains.

The 70 MDR Salmonella strains in Guizhou province were predominantly resistant to antibiotics of 4-5 classes at the same time, accounting for 64.3% of the total, even 4.3% of the strains showed extensive drug-resistance. The 70 MDR Salmonella strains contained 15 sequence types, belonging to 13 eBurst groups, with ST34, ST11, and ST19 being the most common ST types. The most ST type in Tongren City, and unique ST type appeared in some cities, with different dominant ST type with different resistance profiles. The detection of β-lactamase gene showed that 80.0% of the strains carried at least one of β-lactam-resistant genes, with the most common one being bla_TEM (78.6%). The bla_CTX-M gene was detected in 2.9% strains, and the resistant genes subtypes were bla_CTX-M-55 and bla_CTX-M-64. The bla_SHV, bla_OXA-2, and bla_PER genes were not detected.

The MDR Salmonella ST34, ST11 and ST19 as common sequence types in Guizhou province, and there was a certain degree of homology among MDR Salmonella in different regions, and that these strains may have been transmitted in different regions. The β-lactam antibiotics was mainly mediated by bla_TEM.

To analyze the characteristics of epidemiological and pathogenetic trends of HFMD in Guiyang city, and to provide reference for the prevention and control of HFMD.

HFMD cases and pathogenetic surveillance data reported in Guiyang City from 2008 to 2022 were collected through the China Disease Control and Prevention Information System (CDCIS), and a Joinpoint regression model was used to compare the trends and characteristics of HFMD and its pathogenetic changes in populations with different characteristics.

From 2008 to 2022, 124 288 cases of HFMD were reported in Guiyang, with an average annual incidence rate of 187.54/100 000. From 2008 to 2010, the annual incidence rate of HFMD showed a rapid upward trend (APC=144.59%, P=0.002). Slow decline from 2010 to 2022 (APC= -13.07%, P<0.001). Among them, the male incidence rate showed an upward trend from 2008 to 2010 (APC=137.14%, P=0.003), and a downward trend from 2010 to 2022 (APC= -13.54%, P<0.001). The female incidence rate increased rapidly from 2008 to 2010 (APC=157.49%, P=0.001), and showed a downward trend from 2010 to 2022 (APC= -12.41%, P<0.001). Compared with men, women showed a fast rise, slow decline characteristic. Meanwhile, the pathogen analysis results showed that EV71 showed a downward trend, Cox A16 showed a first decrease and then increase trend, and other enterovirus showed an upward trend.

The incidence rate of HFMD in Guiyang is still high, and the trend of annual female incidence rate should be noted. The dominant pathogenic strain of HFMD has changed from EV71 to other EVs. It is suggested that the immunization strategy of HFMD should be adjusted appropriately and pertinently.

To establish a nomogram predictive model for occupational blood exposures of medical staff and validate the model.

From July to December 2023, 20% of the medical staff of each sample hospital were selected for the study using multi-stage whole group probability sampling method and divided into training and validation groups in a 7:3 ratio. LASSO-Logistic regression was used to screen independent risk factors. R language was used to establish a nomogram model and verify it.

A total of 2 251 medical staff were included. There was no significant difference in general data between the two groups (P>0.05). LASSO-Logistic regression analysis showed that post (doctor: OR=3.024, 95% CI: 1.313-6.963; nurses: OR=3.837, 95% CI: 1.739-8.467), professional title (intermediate: OR=1.926, 95% CI: 1.444-2.569); advanced: OR=1.684, 95% CI: 1.052-2.697), education level (undergraduate: OR=2.076, 95% CI: 1.445-2.983); master’s degree or above: OR= 1.767, 95% CI: 1.073-2.910), psychological quality (general: OR=0.658, 95% CI: 0.443-0.987; good: OR=0.568, 95% CI: 0.368-0.879), stress level (moderate: OR=1.348, 95% CI: 1.061-1.713; severe: OR=2.109, 95% CI: 1.457-3.055), protective awareness (general: OR=0.515, 95% CI: 0.332-0.799; good: OR=0.297, 95% CI: 0.186-0.474), protective behavior (sometimes: OR=0.589, 95% CI: 0.363-0.955; always: OR=0.424, 95% CI: 0.261-0.689) was an independent influencing factor of blood-borne occupational exposure. The results of ROC curve showed that the area under the curve of the training group was 0.821 (95% CI: 0.667-0.831), and the validation group was 0.716 (95% CI: 0.618-0.715). The Hosmer-Lemeshow test showed that the calibration of the model was good (P=0.568, 0.956). The calibration curve showed that the prediction curve was basically fitted with the standard curve, and the model prediction accuracy was high. The results of the decision curve showed that the model had the best applicability when the risk threshold was about 0.1-0.8.

The nomogram model can accurately identify high-risk groups of occupational blood exposures and provide a basis for personalized risk prevention and control.

To grasp the current situation and trends of occupational hazards in workplaces of key industries in Ji’an City, and provide a basis for improving the prevention and control of occupational diseases.

The data of occupational hazards in the workplaces of key industries in Ji’an City from 2021 to 2023 were collected from the Workplace Occupational Hazard Monitoring System, and the monitoring results were summarized and analyzed.

A total of 654 enterprises from key industries were recruited as the monitoring subjects in Ji’an City from 2021 to 2023, which were mainly small and micro-scale(94.34%) and manufacturing (74.92%) industries. The exposure ratio of occupational hazards among employees was 55.35%, and the exposure ratio of noise hazard was the highest(47.44%), the second was dust hazard (44.12%). The rates of occupational health training for enterprise leaders, occupational health management personnel, and workers were 72.78%, 72.78% and 83.74%, respectively, which were all increasing gradually year by year (χ²=15.808, 17.649, 398.746, respectively, all P<0.05). The distribution rates of dust masks, anti-poisoning masks or face masks, and noise prevention earplugs or earmuffs were 90.39%, 61.33% and 66.31%, respectively. The distribution rates of dust masks and noise prevention earplugs or earmuffs showed an increasing trend year by year (χ²=24.010, 21.100, respectively, all P<0.05). The rates of installation of dust prevention facilities, anti-poisoning facilities and noise prevention facilities were 88.99%, 76.00% and 54.67%, respectively, which were all increasing year by year (χ²=14.625, 9.818, 34.993, respectively, all P<0.05). There were 437 enterprises exceeded the standard, with an exceeding rate of 66.82%. From 2021 to 2023, the exceeding standard rates of post testing were 40.92%, 24.41%, 24.17%, respectively, which showed a decrease trend year by year (χ²=78.647, P<0.05), and increased with the decrease of enterprise size (χ²=10.093, P<0.05). Among the different kinds of occupational hazard factors, the exceeding rate of noise posts was the highest, the exceeding rates of dust posts and chemical agents’ posts showed a decrease trend year by year (χ²=72.219, 31.342, respectively, all P<0.05).

In recent years, the status of occupational disease hazards in key industries in Ji’an City has been improved, but the situation is still very serious. It is necessary to further strengthen and improve the prevention and control of occupational diseases to protect the occupational health rights and interests of workers.

To investigate the daily dietary nutrient intake and energy expenditure of university students in Sichuan, in order to understand the levels of dietary nutrient intake and physical activity among college students. To evaluate the adequacy of the students’ nutritional requirements by analyzing the intake and energy contribution of the three major macronutrients, as well as the daily energy expenditure and its composition from physical activities.

A convenient sample of 200 college students in a university of Sichuan were included in the study. Body mass index (BMI) and body fat ratio (BFR) were measured using a body composition analyzer. A three-day 24-hour dietary recall method was used to investigate dietary intake, while the Bouchard Physical Activity Diary and triaxial accelerometer AntiGraph wGT3X-BT were used to investigate subjects’ physical activity and energy expenditure.

The median daily energy intake was 2 237 kcal/d for male students and 1 620 kcal/d for female students. The median daily macronutrient intakes for males were: protein 81 g/d, fat 72 g/d, and carbohydrate 295 g/d,and for females: protein 65 g/d, fat 58 g/d, and carbohydrate 210 g/d. The macronutrient energy ratios were 53∶15∶32 for both genders. The median daily energy expenditure was 473 kcal/d for males and 309 kcal/d for females.

Male college students’ daily energy intake and expenditure are significantly higher than females. College students generally show issues of relatively insufficient energy intake, high fat intake, relatively low carbohydrate intake, and insufficient moderate to high-intensity exercise. College students generally lack moderate-to-vigorous physical activity. Students should be encouraged to actively participate in physical activities to promote balanced diets and a healthy lifestyle.

To comprehensively analyze the occurrence, virulence characteristics, and molecular typing of Listeria monocytogenes (L.monocytogenes) isolated from ready-to-eat (RTE) food in Guangxi. By investigating the genetic attributes, antibiotic resistance and potential health risks of L.monocytogenes, this research contributes to our understanding of this pathogen.

We analyzed 158 isolates of L. monocytogenes from RTE foods collected in Guangxi between 2011 and 2020. Whole genome sequencing (WGS) was performed on all isolates and antimicrobial resistance were determined by broth microdilution.The study included the determination of sequence types (STs), serotype, serogroup, lineage, clonal complexes (CC), acquired resistance genes and the presence of L. monocytogenes pathogenicity islands (LIPIs).

Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi. The predominant serotypes were 1/2a, 3a.ST8/CC8 and ST87/CC87 were the dominant sequence types (STs) in RTE foods.All isolates were found to be susceptible to ampicillin(AMP), penicillin(PEN), tetracycline(TET), meropenem(MEM), and trimethoprim-sulfamethoxazole(SXT). Four isolates were resistant to ciprofloxacin. Two isolates wereresistant to erythromycin and vancomycin respectively. Acquired antimicrobial phenotypes were tet (M), msr (D) and mef (A) of an isolate resistant to erythromycin. Most of the isolates carried actA (79.74%), inlF (48.73%), inlK (55.69%)and all isolates carried other virulence genes.46.20%(73/158)of the isolatesharboured 12 virulence genes.It can be a potential public health risk due to its high harboring rate of 17 or 18 virulence genes.

Ready-to-eat meats had the highest detection rate of L.monocytogenes among RTE foods in Guangxi.Acquired resistance genes and hypervirulence genes existed in this bacterium from RTE foods origins, and can be a major risk for consumers and public health.It was suggested that effective food safety measures should be implemented in RTE foods processing and service environments.

To analyze the nutritional intake and growth of infants and young children in the monitoring area of nutrition improvement project in Heilongjiang Province,in order to provide data support for the continuous and scientific promotion of children nutrition improvement project.

Infants and young children aged 6-23 months in 7 project monitoring counties in Heilongjiang Province were selected as the research objects, to monitor the incidence of malnutrition, overweight and obesity, anemia in infants and young children from 2019 to 2023, and to detect their length, weight and hemoglobin value, and analyze the nutrition and growth status of infants and young children.

A total of 10503 infants and young children aged 6-23 months were investigated in 7 monitoring counties from 2019 to 2023. Body weight andmean hemoglobin increased from 10.33 kg and 115 g/L in 2019 to 10.40 kg and 118 g/L in 2023, while body lengthdetection rates of malnutrition and anemia decreased from 8.2% and 29.4% in 2019 to 3.2% and 16.3% in 2023, while the detection rates of overweight and obesity increased from 6.9% in 2019 to 7.5% in 2023, decreasing by 60.98%, 44.56% and -8.70% respectively. Except for the detection rates of malnutrition and overweight obesity at 6-11 months and anemia at 18-23 months, there were significant differences in the detection rates of malnutrition, overweight obesity and anemia in infants of different months from 2019 to 2023(P<0.05).

Through the implementation of the nutrition improvement project for infants and young children aged 6-23 months, the nutritional intake and growth status of infants and young children in the monitoring areas of Heilongjiang Province have been significantly improved, and the project should be further promoted to benefit more infants and young children.

To explore the relationship between the dynamics of plant-based diets and mortality in Chinese older adults.

Based on the data from the Chinese Longitudinal Healthy Longevity and Happy Family Study (CLHLS), older adults who participated in the three surveys from 2011-2018 were selected. The HPDI and UPDI scores of the study participants were calculated in 2011 and 2014, respectively, and they were categorized into four groups based on the median of their two scores: low-low, low-high, high-low and high-high groups. Cox proportional risk regression models were used to analyze the relationship between the dynamics of plant-based diets and mortality in the elderly.

A total of 4 382 subjects were enrolled in this study, and 1 689 (38.5%) of them died during the 7-year follow-up period. The results of Cox proportional hazards regression model showed that after adjusting for confounding factors such as gender, age, and education, the risk of death in the high-high HPDI group was reduced by 17% (HR=0.83, 95% CI: 0.72-0.97), while the risk of death in the high-high UPDI group was increased by 29% (HR=1.29, 95% CI: 1.11-1.49).

Older adults who maintain a healthy plant-based diet have a lower risk of death.

To observe the effects of breastfeeding on intestinal flora and short-chain fatty acids (SCFAs) in infants and how they affect the occurrence of infant allergies.

A nested case-control study was conducted in an existing mother-infant cohort. A total of 48 infants were selected as study subjects and divided into a healthy/allergic mixed feeding group and a breastfeeding group. Fecal samples were collected at 15 days, 1 month, 6 months, and 12 months. The contents of three SCFAs in the samples were determined by gas chromatography-mass spectrometry (GC-MS), and the bifidobacteria in infant feces were quantitatively analyzed by real-time fluorescent quantitative PCR.

Feeding patterns mainly affected bifidobacteria at 15 days and 1 month. The bifidobacteria level in healthy mixed-fed infants was significantly higher than that in allergic mixed-fed infants at 15 days (7.75±0.60 CFU/g vs 6.28±0.28 CFU/g, t=2.232, P=0.047). At 1 month, the bifidobacteria level in healthy mixed-fed infants was higher than that in healthy breastfed infants (8.01±0.50 CFU/g vs 6.62±0.13 CFU/g, t=2.712, P=0.027). There were differences in the main SCFAs between healthy and allergic infants with different feeding patterns. On day 15, the acetic acid content in the healthy breastfeeding group was significantly higher than that in the allergic breastfeeding group and the healthy mixed feeding group (1 035.10 μg/g vs 285.55±90.64 μg/g, t=3.698, P=0.034; 1 035.10 μg/g vs 406.11±63.19 μg/g, t=3.148, P=0.014), and the propionic acid content in the healthy mixed feeding group was higher than that in the allergic mixed feeding group (128.00±32.24 μg/g vs 12.61±3.90 μg/g, t=3.553, P=0.007). At 6 and 12 months, the propionic acid content in the healthy mixed feeding group was lower than that in the allergic mixed feeding group. There were varying degrees of correlation between SCFAs and bifidobacteria in the intestines of healthy and allergic infants with different feeding patterns. In the healthy group, a simple and stable relationship between bifidobacteria and SCFAs was observed in mixed-fed infants, while in the allergic group, the relationship between SCFAs and bifidobacteria was complex and had no obvious pattern.

Feeding patterns affect the occurrence of infant allergies by influencing intestinal flora and SCFAs in infants. Mixed-fed infants are more prone to allergies, while breastfeeding has a significant protective effect on the intestines of allergic infants.

To explore the correlation between adolescents’ self-assessment of oral health and oral examination results assessed according to WHO-recommended standards and to analyze the factors influencing adolescents’ self-assessment of oral health.

A total of 2 146 adolescents aged 12 from 11 districts and counties in Guizhou Province were selected through multi-stage random sampling. Oral health examinations and a questionnaire survey were conducted according to the methods and standards of the Fourth National Oral Health Epidemiological Survey. Kendall correlation analysis was used to compare the correlation between the results of the two evaluation methods. The chi-squared (χ²) test and ordered logistic regression analysis were employed to analyze the factors affecting adolescents’ self-assessment of oral health status.

There was a significant correlation between self-rated oral health and oral examination results. Statistical differences were observed in adolescents’ self-rated oral health based on different economic regions, brushing habits, general health evaluations, experiences of dental trauma, toothache, and the number of siblings (P<0.05).

Adolescents’ self-assessment of oral health can reflect their actual oral health status. It is recommended to focus on the key factors affecting adolescents’ oral health, promote and popularize proper brushing techniques, and improve the utilization rate of oral health services.

To study the effects of green space exposure combined with outdoor exercise duration on negative emotions (depression, anxiety and stress) among primary and secondary school students in Jiangsu Province.

A total of 19 903 primary and secondary school students in Jiangsus Province who met the criteria were randomly selected for inclusion in this study. Through the questionnaire survey, the demographic data of the research subjects and the information of negative emotions were collected. The annual average value of the Normalized Vegetation Index (NDVI) of the school environment of the study subjects was used as the spatial measurement of green space. A logistic regression model was used to evaluate the association between green space exposure and outdoor exercise duration and negative emotions.

The detection rates of depression, anxiety and stress were 13.2% (2 631), 21.9% (4 367) and 9.7% (1 927) of the 19 903 participants, respectively. The range of NDVI is (0.19, 0.52); The correlation between green space exposure and outdoor exercise duration, individually or in combination, and the detection rate of negative emotions was generally similar in both regional and gender subgroups.

Expanding the exposure of green space and increasing the time of outdoor exercise can help alleviate the negative emotions of primary and secondary school students, and the research on green space exposure can provide effective measures for school health and psychological services.

To explore the current situation and influencing factors of medical students’ sleep quality, and to provide targeted intervention measures for improving sleep quality.

A questionnaire survey was conducted among 2884 medical students from September 2021 to January 2022 using a convenience sampling method. Multiple correspondence analysis and multivariate logistic regression analysis were used to explore the influencing factors of medical students’ sleep quality.

The prevalence of poor sleep quality among medical students was 70.32%. The results of multiple correspondence analysis showed that female medical students, sophomores, those with poor self-rated interpersonal relationships and poor health-promoting lifestyles were more likely to have poor sleep quality. The results of multiple stepwise logistic regression analyses showed that four independent variables entered the regression equation, gender (OR_female=1.34,95% CI: 1.13-1.60), grade (OR_sophomore=2.23,95% CI: 1.72-2.88), self-rated interpersonal relationships (OR_poor=1.76,95% CI: 1.47-2.12) and health-promoting lifestyle (OR_poor=2.02, 95% CI: 1.70-2.39).

The prevalence of poor sleep quality among medical students is at a high level. There is a need to focus on sophomores and female students, and to take targeted interventions from the aspects of interpersonal relationship improvement and healthy lifestyle development to improve sleep quality.

To understand the adverse childhood experiences of middle school students in Huangpu District and the association and gender differences between sleep disorders, and to provide scientific basis for the prevention and control policies of sleep disorders.

A stratified cluster sampling method was used to investigate the adverse childhood experiences (ACEs) of middle school students, evaluate their overall sleep function, and investigate the childhood adverse experiences of 6 middle school students in Huangpu District. The detection rate of sleep disorders in different childhood adverse experiences exposure was compared using a chi square test. A binary logistic regression model was established to analyze the risk and gender differences between exposure to adverse childhood experiences and sleep disorders in middle school students.

This study analyzed a total of 6 710 students from 6 high schools in Huangpu District, Guangzhou, with 3 525 males and 3 185 females. The detection rate of sleep disorders was 18.0%. The detection rate of ACEs ranged from 1.3% (substance abuse among family members) to 38.9% (domestic violence). The results of the multivariate binary logistic regression analysis showed that, except for the parental disability dimension, the risk of developing sleep disorders was increased in subjects with ACEs (OR=1.38-4.58). There was a dose-response relationship between the cumulative number of ACEs and the risk of sleep disorders. In addition, the risk intensity between emotional neglect and sleep disorders was significantly higher in girls than in boys, but there was no interaction between gender and the association between other types of ACEs or the cumulative number of ACEs and sleep disorders.

There is a significant association between ACEs exposure and sleep disorders among middle school students in Huangpu District, the incidence of sleep disorders in girls is higher than that in boys, and girls who were emotionally neglected during childhood have a higher risk of developing sleep disorders.

To understand the current status of psychological therapy and counseling services in mental health institutions in mainland China so as to provide reference basis for developing subsequent relevant policies.

From May to June 2021, the National Mental Health Project Office conducted a survey using a self-designed questionnaire on 3 833 institutions providing psychological therapy and counseling services in 31 provinces in mainland China. The data was collected by the mental health project offices at all levels and submitted to the National Mental Health Project Office.

2 214(57.76%) institutions provided psychological therapy or counseling services to both outpatient and inpatient patients. In 2020, we served over 21.86 million people, with outpatient patients slightly higher than inpatients. 2 685(70.05%) institutions charge fees, of which 1 206(44.92%) reported that their psychological therapy services are included in medical insurance. The fee standards for psychological therapy and counseling services vary greatly among different institutions, with psychological therapy services ranging from 5 yuan RMB to 3 000 yuan RMB per session and psychological counseling services ranging from 5 yuan RMB to 2 400 yuan RMB per session.

Residents have low utilization of psychological therapy or counseling services within medical institutions. The prices of psychological therapy or counseling services vary greatly, and the medical security needs to be deeply implemented.

To analyze the data of public consultation events and corresponding online public opinion on disease prevention and control in Chengdu from 2017 to 2023, so as to provide reference for understanding the public’s demand for public health information and reducing the occurrence of adverse public opinion in disease prevention and control and response to emergency public health events.

Collect information on public consultation events related to Chengdu Center for Disease Control and Prevention from 2017 to 2023, and use Baidu search tool “Baidu Index” to collect public opinion monitoring data during the same period. Statistical analysis was conducted on the distribution of public consultation events and their correlation with the Baidu Index of corresponding keywords using methods such as statistical description, chi square test, and linear correlation.

From 2017 to 2023, a total of 7 253 public consultation incidents were received, with the highest number in 2022 (4 915); 12345 hotline platform calls are the main source, accounting for 58.78% (4 263 cases); The main types of consultations from 2017 to 2019 were vaccination issues; From 2020 to 2023, the consultation related to COVID-19 will account for 90.41% (5 952/6 583), and the questions related to the prevention and control of COVID-19 (χ²=1 092.147, P<0.001) and COVID-19 nucleic acid detection (χ²=45.083, P<0.001) in 2022 have increased respectively compared to 2020-2021; In 2023, the proportion of non-COVID-19 related vaccination issues and disease prevention and control issues will decrease (χ²=459.109, P<0.001) and increase (χ²=13.999, P<0.001) respectively compared with that before COVID-19; Linear correlation analysis showed that the number of questions related to vaccination between the “vaccination” search index (r=0.864, P=0.027), the number of questions related to COVID-19 prevention and control between the “COVID-19” search index (r=0.794, P=0.033) and information index (r=0.872, P=0.010), the number of questions related to COVID-19 vaccination between the “COVID-19 vaccine” search index (r=0.840, P=0.018) and information index (r=0.835, P=0.019), and the number of questions related to COVID-19 nucleic acid detection between the “nucleic acid detection” information index (r=0.972, P<0.001) were positively correlated respectively.

Using public health professional knowledge to answer questions for the public is a targeted form of health education. In daily work, we should pay more attention to public opinion monitoring, understand public needs through multiple channels, strengthen public opinion guidance and risk prediction, continue to carry out publicity and education, and improve public health literacy; When an emergency public health event occurs, it is necessary to promptly disclose the epidemic situation, strengthen prevention and control propaganda and guidance, inform the public of response methods, and continuously optimize prevention and control measures to provide support for disease prevention and control and response to emergency public health events.

To analyze the influencing factors of preventive service cost in Guizhou Province from 2016 to 2022 by using grey correlation degree method.

Based on the accounting results of “SHA2011” and the data of “Guizhou Statistical Yearbook” and “Guizhou Health Statistical Yearbook”, the factors affecting the cost of preventive services in Guizhou from 2016 to 2022 were analyzed by using grey correlation method.

The highest correlation with the cost of preventive services in Guizhou province was the number of health technicians in epidemic prevention and control and maternal and child health institutions (0.826), followed by the gross enrollment rate of higher education (0.735), the number of health technology beds in epidemic prevention and control and maternal and child health institutions (0.730). The lowest correlation was the proportion of tertiary industry in GDP (0.593).

Health resource input has the strongest correlation with the cost of preventive services. Health fund input and public health services are the main influencing factors of the cost of preventive services in Guizhou Province. At the same time, full attention should be paid to the influence of social factors such as economic development, education level, urbanization level and age structure on the cost of preventive services.

To explore the current situation and development trend of coupling and coordination of medical resource allocation and service utilization in traditional Chinese medicine hospitals, to clarify the coordinated development relationship between the two, and to provide reference opinions for promoting the benign interaction between the two and achieving balanced development.

From 2017 to 2021, 11 relevant index data of medical resource allocation and service utilization of TCM hospitals in 30 provinces of China were selected, and the comprehensive development index and relative development degree were used to comprehensively evaluate the allocation and service utilization of TCM hospitals, and the coupling coordination method was used to explore the coupling and coordination relationship and development trend of the two systems.

From 2017 to 2021, the coupling coordination degree of medical resource allocation and service utilization of TCM hospitals in various regions of China decreased, showing a stepwise decreasing pattern from east to west. There were obvious differences in the comprehensive development level of medical resource allocation of traditional Chinese medicine hospitals among regions, and the lag in medical resource allocation had become a constraint for the coordinated development of the western region. The overall structure of the relative development level had changed from “8-13-9” in 2017 to “8-12-10” in 2021, and only 12 provinces would achieve coordinated development.

There is a lot of room for improvement in the coupling and coordination of resource allocation and service utilization of traditional Chinese medicine hospitals in China, and the allocation level of medical resources in traditional Chinese medicine hospitals should be optimized according to local conditions, so as to promote the high-quality development of traditional Chinese medicine hospitals with economy. The improvement strategy of coupling coordination level is dynamically adjusted according to the relative development degree, so as to promote the benign interaction between the two and achieve high-quality coordinated development.

To explore strategies to address the gap and uneven distribution of general practitioner(GP) resources from a provincial perspective, aiming to meet the growing medical needs of the people and advance the reform of the medical and health system.

Based on the provincial GP data from 2013-2022, Kernel density estimation was used to reveal the dynamic evolution of GP resources, while the spatial autocorrelation method was applied to explore their spatial correlation characteristics. Additionally, a spatial econometric model was used to investigate the direct impacts and spillover effects of the factors influencing general practitioner resources.

There is a positive autocorrelation in China’s general practitioner resources. Among the direct effects, urbanization rate and population density have a positive impact on general practitioner resources, while the elderly dependency ratio has a negative impact. In terms of indirect effects, urbanization rate, population density, the proportion of medical and health expenditure in fiscal expenditure, and the number of medical and health institutions all have a negative impact on general practitioner resources.

General practitioner resources in China are not randomly distributed spatially, and the spillover effects of influencing factors in adjacent areas can also impact general practitioner resources. It is recommended to focus on collaboration of medical resources and coordination of medical policies among different regions, promoting cross-regional communication and sharing of general practitioners.

To analyze the chain-mediated roles of intergenerational spiritual support and depression between social participation and cognitive functioning in older adults.

In this paper, we used data from the China Health and Aging Tracking Survey (CHARLS) 2020 to screen 3 737 study participants who met the criteria. Depression and cognitive function were measured using the Center for Streaming Depression Scale (CES-D 10) and the Overall Cognitive Scoring Instrument, respectively. Two-column correlation analyses (Pearson’s correlation analyses) of correlation were used, and multiple mediating effect models were constructed to investigate the mediating effects of intergenerational spiritual support and depression in the impact of social participation activities and cognitive functioning in older adults.

After including control variables such as age, gender, marital status, chronic disease prevalence, and insurance participation, regression analysis showed that older adults’ social participation status had a significant direct effect on their cognitive functioning (β=0.254, P<0.001), while intergenerational spiritual support (β=0.052, P<0.050) and depression status (β= -0.084, P<0.001) also had a significant effect on cognitive function in older adults. The results of mediation effect analysis showed that the simple mediation effects of intergenerational psychiatric support and depression status on the relationship between social participation status and cognitive function in older adults were 0.006(95% CI: 0.001-0.012) and 0.020(95% CI: 0.006-0.036), respectively, and the chained mediation effect was 0.001(95% CI: 0.001-0.004).

This study finds a direct mediating effect of depressive status and intergenerational psychiatric support between social participation status and cognitive functioning in older adults, as well as a chain-mediated effect between the two. Social participation of older adults should be improved, while their cognitive function can be improved by children caring more about their parents and reducing depressive symptoms to delay or prevent the occurrence of cognitive impairment in older adults.

To investigate the causal association between sarcopenia and hypertension and the mediating role of sedentary behavior using Mendelian randomization.

The summary statistical data of sarcopenia-related indicators,sedentary behavior (television watching,computer use and driving) and hypertension were extracted from IEU Open GWAS database website.Univariate Mendelian randomization was used to explore the association between the indicators of sarcopenia and hypertension, and two-step Mendelian randomization was used to investigate whether sedentary behaviors (television watching, computer use and driving) mediated the causal association.

Univariate Mendelian randomization revealed that all four indicators of sarcopenia were negatively associated with hypertension, including appendicular lean mass (OR=0.92, 95% CI: 0.88-0.96), left grip strength (OR=0.78, 95% CI: 0.66-0.92), right grip strength (OR=0.78, 95% CI: 0.66-0.91) and walking pace (OR=0.20, 95% CI: 0.14-0.30). Furthermore, two-step Mendelian randomization showed that television watching partially mediated the causal association between left grip strength (22.92%) and right grip strength (23.62%) and hypertension, respectively.

All four indicators of sarcopenia had negative causal associations with hypertension and that television watching mediated part of the causal association between left and right grip strength and hypertension.

To analyze the relationship between cardiovascular-metabolic complications andbasic activity of daily living(BADL) and instrumental activities of daily living (IADL) in Chinese middle-aged and elderly individuals, and to explore the factors influencing different types of ADLs in this demographic with cardiovascular-metabolic complications.

The effects of cardiovascular-metabolic co-morbidities on ADLs among middle-aged and elderly individuals were investigated using the χ² test and logistic regression model in a sample of individuals aged 45 and older from the 2020 China Health and Elderly Care Tracking Survey.A randomized forest prediction model was developed to investigate the influencing factors of different types of activities of daily living in middle-aged and elderly people with cardiovascular-metabolic co-morbidities, and to predict the importance of each influencing factor.

Among China’s middle-aged and elderly population, 19.75% had at least one cardiovascular metabolic disease (CMD), and 4.18% had cardiovascular metabolic co-morbidities (CMM). The prevalence of BADL impairment was 15.90%, and that of IADL impairment was 17.32%.After adjusting for confounding factors like age and sex, middle-aged and older adults with cardiovascular metabolic co-morbidities had a higher risk of developing impaired ADLs compared to their counterparts without these co-morbidities (BADL: OR=1.662, 95% CI: 1.246-2.216; IADL: OR=2.186, 95% CI: 1.663-2.874).Age, depression, pain distress, life satisfaction, literacy, and participation in social activities were predictive of impaired BADL and IADL in older adults with multiple cardiovascular-metabolic co-morbidities, with good predictive modeling (BADL: AUC=0.804; IADL: AUC=0.761).

Middle-aged and older adults with multiple cardiovascular-metabolic co-morbidities are more likely to experience impairments in ADLs. Healthcare providers should consider the specific conditions and risk factors of this demographic and develop personalized, multidimensional intervention programs focusing on mental health, pain and chronic disease management, and life satisfaction enhancement to prevent ADL decline in those with cardiovascular-metabolic co-morbidities.

To systematically evaluate the incidence of self-harm behavior and self-harm ideation among HIV/AIDS patients, providing evidence-based support for effectively improving their mental health.

A comprehensive search of relevant studies was conducted in databases including CNKI, CBM, VIP, WanFang Data, PubMed, Embase, Cochrane Library, Web of Science, and PsyclNFO, covering literature up to August 20, 2024. Two researchers independently screened the literature, extracted data, and assessed the quality. Meta-analysis was performed using Stata 17.0 software.

A total of 9 studies were included, with a sample size of 17 414. Meta-analysis of the random effects model showed that the combined incidence of self-harm behaviors and self-harm ideation in HIV/AIDS patients was 12.3% (95% CI: 9.2%-15.4%), the incidence of self-harm behaviors was 12.4% (95% CI: 7.2%-17.6%), and the incidence of self-harm ideation was 13.3%(95% CI: 8.2%-18.5%). Subgroup analyses showed that the prevalence of self-harm behaviors and self-harm ideation was higher among HIV/AIDS patients in Asia and was higher in moderate-quality studies than in high-quality studies. In addition, higher prevalence rates were also observed in studies published before 2015, in people aged 18-24 years, and in studies with <50% males, but these results need to be interpreted with caution in conjunction with the results of the heterogeneity analysis.

The incidence of self-harm behavior and self-harm ideation is relatively high among HIV/AIDS patients, particularly in Asia. Further research is needed to validate differences among various groups and to develop effective intervention strategies.

To comprehensively analyze the influencing factors of the blood plate-paper bridge method for H antigen induction and identification, and to investigate its mechanism in order to promote the application of this method in Salmonella serotyping.

The experimental conditions of the blood plate-paper bridge method for Salmonella serotyping were optimized by comparing the effects of different paper bridge materials on the induction and identification of H antigen, scanning the surface of different paper bridge materials by scanning electron microscope to explore the mechanism of the effects, and combining the factors of environmental humidity and saline dosage. The optimized method was compared with the simple plate method of GB 4789.4 standard for serotyping 40 strains of Salmonella.

The matte surface of CN110 and CN95 nitrocellulose membranes had similar bridging effect with ordinary filter paper, and the difference was not statistically significant (P>0.05), while the effect of filter membrane was significantly lower than that of ordinary filter paper (P<0.05). Salmonella spread better at higher humidity, and there was a significant difference between the results at 80% humidity compared with those of the 50% and 20% groups (P<0.016 7). The spreading effect was significantly improved with increasing amount of saline (P<0.001), and the optimal amount was 15 μL. Forty foodborne Salmonella strains were identified using the two methods, and the method in this study was able to accurately identify Salmonella serotypes in one go, whereas the simple plate method required one to three inductions.

The blood plate-paper bridge method optimized in this study is easy to operate and has a significant induction effect, which is suitable for the identification of Salmonella serotypes. This method can be used as an effective complement to Salmonella serotyping methods to improve the efficiency and accuracy of foodborne disease traceability.

To construct a Propionibacterium acnes (P. acnes)-induced cellular inflammation model and to study the anti-inflammatory effect of a thermal water.

Utilize human immortalized keratinocytes (HaCaT) was used in the experimentation. Co-incubate the cells with thermal spring water and employ the CCK-8 assay to assess cell viability, screening for the optimal intervention time for the thermal spring water. Expose the cells to varying concentrations of P. acnes for different times, measure cell viability, calculate the 50% inhibitory concentration (IC₅₀) of P. acnes, and determine the model induction conditions by testing IL-6 levels. The cells were soaked in thermal spring water for 0.5 hours, followed by co-culturing with P. acnes as the preventive intervention group, or co-culturing with P. acnes first and then soaking the cells in hot spring water for 0.5 hours as the therapeutic intervention group. The levels of cell viability, pro-inflammatory cytokines, and gene expression of inflammation pathways were assessed using the CCK-8 assay, ELISA, and RT-PCR, respectively. Concurrently, control groups with saline solution, tap water, and pure water, along with model and blank controlswere set up.

After treating the cells with thermal spring water and physiological saline for 0.5 hours, cell viabilities were 92.7% and 99.09%, respectively, which led to the selection of 0.5 hours as the intervention time for subsequent experiments.P. acnes at an OD₆₀₀ of 0.25 was co-incubated with the cells for 5 hours to establish the model. In the preventive intervention, the secretion levels of IL-1β and IL-8， as well as the relative expression levels of IL-6, TNF-α, IL-8, and the genes TLR2 and NF-κB in the cells of the thermal spring water group, were all lower than those in the model group (P<0.05), and significantly lower than those in the saline solution group. In the therapeutic intervention, the contents of IL-6, IL-1β, TNF-α, and IL-8, as well as the expression levels of the genes IL-6, IL-1β, TNF-α, TLR2, and NF-κB in the thermal spring water group, were all lower than those in the model group. The contents of IL-6, IL-1β, TNF-α, and the expression levels of the genes IL-6, IL-1β, IL-8, TLR2, and NF-κB were also significantly reduced compared to those in the saline solution group (P<0.05).

This thermal water may exert its anti-inflammatory effect by down-regulating the expression of TLR2 and NF-κB genes in inflammatory signalling pathway and by reducing the secretion of pro-inflammatory cytokines.

To identify the trajectories of sleep duration among adults in China, and explore the associatedinfluencingfactors.

Data was derived from 2010-2020 China Family Panel Studies (CFPS). Self-reported measures of night sleep duration were used. People aged ≥18 years were included. LCGM and polynomial Logistic regression model were used.

A total of 10 113 adults were included. Four similar trajectory groups of sleep duration were identified among Chinese adults: severely insufficient sleepduration group (3.12%), insufficient sleepduration group (27.63%), moderate sleepduration group (63.41%) and excessive sleepduration group (5.84%). Polynomial logistic regression showed that, using moderate sleep duration group as the reference, individuals who were older (OR=7.29, 95% CI: 2.23-23.83), lived in urban areas (OR=2.45, 95% CI: 1.89-3.17), had higher levels of depressive symptoms (OR=1.12, 95% CI: 1.09-1.15), and people who drank alcohol (OR=1.60, 95% CI: 1.10-2.32) were more likely to be classified into severely insufficient sleep duration group. Males (OR=0.49, 95% CI: 0.35-0.67), living in western regions (OR=0.42, 95% CI: 0.29-0.61) were less likely to be classified into severely insufficient sleep duration group. Older adults (OR=2.38, 95% CI: 2.01-2.83), those with middle school education (OR=1.16, 95% CI: 1.03-1.30), high school education (OR=1.20, 95% CI: 1.04-1.40), college education and above (OR=1.23, 95% CI: 1.01-1.50), living in urban areas (OR=1.34, 95% CI: 1.21-1.48), having more depressive symptoms (OR=1.03, 95% CI: 1.02-1.04), adults drinking alcohol (OR=1.20, 95% CI: 1.05-1.37), and using Internet (OR=1.20, 95% CI: 1.07-1.34) were more likely to be classified into insufficient sleep duration group. Those with higher economic status (OR=0.84, 95% CI: 0.70-1.00), living in western regions (OR=0.51, 95% CI: 0.45-0.58), self-reported fair health (OR=0.86, 95% CI: 0.75-0.98) and good health (OR=0.83, 95% CI: 0.71-0.97), rated life satisfaction as fair (OR=0.74, 95% CI: 0.57-0.94) and satisfied (OR=0.76, 95% CI: 0.60-0.97) were less likely to be in insufficient sleep duration group. Living in the western regions (OR=1.81, 95% CI: 1.44-2.27) were more likely to be classified into excessive sleepduration group, while those aged 30-44 years (OR=0.64, 95% CI: 0.48-0.84), 45-59 years (OR=0.74, 95% CI: 0.55-0.99), having middle school education (OR=0.75, 95% CI: 0.61-0.94), college education and above (OR=0.25, 95% CI: 0.14-0.46), living in urban areas (OR=0.73, 95% CI: 0.60-0.88) were less likely to classified into excessive sleepduration group.

Four groups of sleep duration trajectories among Chinese adults were identified. Sociodemographic factors, socioeconomic status, physical & mental health, health behavior, and life satisfaction were closely related to the trajectories of sleep duration among Chinese adults.

To analyze the characteristics of Adverse Events Following Immunization (AEFI) of domestic rotavirus (live) vaccine (RVVO) in Shaanxi province from 2018 to 2022, and to evaluate the safety of RVVO vaccination.

The AEFI case data on RVVO vaccination in Shaanxi Province between 2018 and 2022 was gathered from the China AEFI Information Management System., and the vaccination situation of RVVO in Shaanxi province was collected through the immunization planning information management system of Shaanxi province. The analysis was conducted using the descriptive epidemiological technique.

A total of 461 102 doses of RVVO were inoculated in Shaanxi province from 2018 to 2022, and 48 cases of AEFI were recorded, with an incidence of 10.41 cases per 100 000 doses, including 43 cases of normal reactions (9.33 cases per 100 000 doses) and 3 cases of abnormal reactions (0.65 cases per 100 000 doses). 2 cases of coupling disease and No serious AEFI cases recorded. The three abnormal reactions were allergic rash (0.22/100 000 doses), acute diarrhea (0.22/100 000 doses) and rotavirus diarrhea (0.22/100 000 doses). 97.92% of the reported AEFI cases were cured or improved after follow-up, and no sequelae and death cases were reported. The age of onset of AEFI was less than 1 year old and occurred on the day of inoculation. The incidence of AEFI at the first, second and third doses was 12.65 cases per 100 000 doses, 10.64 cases per 100,000 doses and 361 cases per 100 000 doses, respectively. RVVO combined with other vaccines reported a general reaction rate of 10.16 per 100 000 doses and an abnormal reaction rate of 0.97 per 100 000 doses in AEFI.

The domestic oral live attenuated rotavirus vaccine has good safety, and the timely vaccination rate of children should be further strengthened.

To examine the relationship between the increase in chronic diseases, self-rated health, and depressive tendencies among middle-aged and older adults in China.

Using data from the 2015 and 2018 waves of CHARLS, this study investigated the mediating role of self-rated health and depressive tendencies in the relationship between the increase in chronic diseases among 12 637 Chinese adults aged 45 and above. The mediation analysis was conducted using the PROCESS 4.0 program and validated through the Bootstrap method.

The prevalence of depressive tendencies among Chinese adults aged 45 and above was 37.11%. After controlling for relevant variables, the increase in chronic diseases significantly impacted both self-rated health and depressive tendencies (c value: t = 30.984, P<0.001; a value: t=17.072, P<0.001). When self-rated health was introduced as a variable, both the increase in chronic diseases and self-rated health significantly affected depressive tendencies (c’ value: t=8.915, P<0.001; b value: t=30.629, P<0.001). Self-rated health partially mediated the relationship between the increase in chronic diseases and depressive tendencies, with the highest effect proportion of 47.74%.

The increase in chronic diseases among middle-aged and older adults can influence depressive tendencies through self-rated health. To reduce depressive tendencies in these patients, it is essential to effectively improve their self-rated health.

To comprehensively assess the disease severity in patients with Wilson’s disease (WD) who had been previously diagnosed and treated with copper removal by Unified Wilson’s Disease Rating Scale (UWDRS) and analyze its influencing factors, in order to provide a reference for patient management and improvement of patients’ health.

Wilson’s disease patients who were admitted to West China Fourth Hospital of Sichuan University from July 2023 to March 2024 were selected as the study subjects, and all patients have been diagnosed according to the diagnostic criteria. The survey was conducted using self-designed structured questionnaires and UWDRS scales. Univariate analysis and multiple linear regression analysis were used to analyze the disease severity and influencing factors of patients with WD who had been previously diagnosed and treated with copper removal.

A total of 103 patients with eligible and complete data were included. The total UWDRS score of patients with WD who underwent copper repulsion therapy was 27.0 (11.5, 60.5), neurological symptom score of 21.0 (4.5, 48.5), hepatic symptom score of 3.0 (1.0, 4.0), and psychiatric symptom score of 5.0 (2.0, 8.0). More than 50% of patients still have symptoms or signs of rapid alternating movements of hand, finger tapping, salivation, gait abnormalities, speech impairment (dysarthria), impaired handwriting, and other bleeding or hematoma. Multiple linear regression analysis showed that education level, classification of first-time diagnosed disease, low-copper diet, social support and medication adherence were the influencing factors of UWDRS total score (P<0.05). The higher the patient’s level of education, medication adherence, and social support are, the lower the UWDRS score is. Patients with hepatic type had lower scores than those with cerebral type, and those who tried to adhere to a low-copper diet had lower scores than those who adhered to a low-copper diet all the time.

More than 50% of the previously diagnosed patients with WD who have been treated with copper removal still have different degrees of neurological symptoms, and the treatment of neurological symptoms needs to be further improved. Education level, classification of first-time diagnosed disease, low-copper diet, social support, and medication adherence were the influencing factors of disease severity in patients with WD.