To investigate the association between lifestyle and the incidence and development trajectory of cardiometabolic multimorbidity.

Based on the China Health and Retirement Longitudinal Study(CHARLS) from 2011 to 2020, a total of 8 202 individuals aged 45 years and older were included. A Multi-state Markov model (MSM) was applied to analyze the effects of lifestyle on the trajectories of cardiometabolic multimorbidity.

The disease progression trajectories mainly involved transitions from health to first cardiometabolic diseases (FCMD), health to cardiometabolic multimorbidity (CMM) and FCMD to CMM. During the follow-up period, 2 962 participants developed FCMD, 687 participants developed CMM, and 699 participants died. The results indicated that transitioning from health to FCMD, being overweight or obese, having longer nighttime sleep duration, and lacking a habit of daytime naps were positively associated with FCMD, which were 1.40(95%CI: 1.30-1.52) times, 1.16(95%CI: 1.02-1.32) times, and 1.21(95%CI: 1.06-1.39) times higher than those of healthy lifestyle people, respectively. During the transition from health to CMM, being overweight or obese was positively associated with the increased incidence of CMM (HR=1.42,95%CI: 1.06-1.90). Transitioning from FCMD to CMM, only overweight or obesity was observed as a risk factor (HR=1.44,95%CI: 1.17-1.78).

Lifestyle may increase the risk of cardiometabolic diseases and multimorbidity, emphasizing the importance of lifestyle interventions in health management practices.

To analyze the causal relationships between basal metabolic rate(BMR) and frailty index (FI) based on bidirectional two-sample mendelian randomization(MR).

The data of BMR (n=454 874) and FI (n=175 226) were extracted from the published genome-wide association studies (GWAS). By setting conditions with P<5×10^-8 as the screening criterion, the linkage disequilibrium coefficient set to 0.001 and the width of the linkage disequilibrium region of 10 000 kb, and single nucleotide polymorphisms (SNP) were screened out as the final instrumental variables. Five methods including MR-Egger regression, weighted median, Inverse variance weighted (IVW), simple mode and weighted mode were used for MR analysis, and IVW was used as the main analysis method, and β and 95%CI were used to demonstrate the causal relationship between BMR and FI. MR-Egger intercept and MR-PRESSO analysis were used to test the pleiotropy, and the heterogeneity was analyzed by Cochran Q test of MR-Egger. Leave-one-out test were performed to analyze the sensitivity. Finally, reverse MR analysis was used to verify the robustness of the results.

A total of 787 SNPs associated with BMR and 9 SNPs associated with FI were screened. The MR analysis showed a causal relationship between BMR and FI(Weighted median, β=0.105, 95%CI: 0.063-0.146, P<0.001; IVW, β=0.110, 95%CI: 0.808-0.140, P<0.001; weighted mode, β=0.145, 95%CI: 0.047-0.244, P=0.004). The screened SNPs did not have horizontal pleiotropy because the the MR-Egger intercept was 0.000 2 (P=0.374), Therefore, the MR was an effective method for causal inference in this study. The Cochran Q-test results showed Q=137.053, P=0.188, indicating no heterogeneity among the SNPs included in the MR analysis. And no outlier SNPs were found by MR-PRESSO analysis. The sensitivity analysis based on the leave-one-out method showed that the individual SNP did not affect the robustness of the MR analysis results. No causal relationship between FI and BMR was found in the reverse MR analysis(P>0.05).

Genetically predicted high BMR level is significantly associated with elevated FI, and there is no association in reverse direction, which may provide fresh ideas for proposing feasible interventions to manage frailty.

To investigate heterogeneous trajectory of sarcopenia and its associated factors among older adults with multimorbidity.

Based on the Asian Working Group for Sarcopenia 2019 Consensus, three waves of data in 2011, 2013, and 2015 from the China Health and Retirement Longitudinal Study were utilized. Latent growth mixture model and multinomial logistic regression were selected to identify heterogeneous sarcopenia trajectories and associated factors among older adults with multimorbidity.

Of the 1 254 participants, four heterogeneous trajectories of sarcopenia were identified: high initial but sharply declining trajectory (7.5%,n=93), low and stable change trajectory (64.0%, n=803), high and stable change trajectory (23.5%, n=295), low initial but sharply increasingtrajectory (5.0%, n=63). Disability, body mass index (BMI), cognition, sleep duration, smoking habits, and alcohol consumption were associated with different sarcopenia trajectories.

There are heterogenous sarcopenia trajectories among older adults with multimorbidity, targeted intervention regarding disability, BMI, cognition, sleep, smoking, and drinking should conducted to prevent sarcopenia.

To assess the potential causal relationship between gut microbiota and the risk of uterine fibroids using a Mendelian randomization approach.

We used the genome-wide association study (GWAS) statistics of gut microbiota published by the Netherlands Microbiome Project in 2022 (n=7 738) and the GWAS data of uterine fibroids from the Finnish R9 database (31 661 uterine fibroid patients and 179 209 controls). Inverse variance weighting (IVW), MR-Egger regression and weighted median method were used to study the causal relationship between intestinal flora and uterine fibroids. Sensitivity analyses were performed to test the reliability of the results of the Mendelian randomization analysis.

IVW results showed that Bacteroides (OR=1.045, 95%CI: 1.006-1.085), Ruminococcaceae (OR=1.119, 95%CI: 1.027-1.219), Roseobacter(OR=1.074, 95%CI: 1.004-1.149), Lactobacillus (OR=1.066, 95%CI: 1.017-1.118) and Firmicutes (OR=1.052, 95%CI: 1.003-1.102) were positively correlated with the incidence of uterine fibroids. No significant statistical difference was foundafter FDR correction (P>0.05).Bifidobacterium was negatively correlated with the incidence of uterine fibroids (OR=0.952,95%CI: 0.921-0.985). The causal association was statistically significant after FDR correction (P<0.05). No significant heterogeneity and horizontal pleiotropy were found in the sensitivity analysis.

Bifidobacterium may be a protective factor for the pathogenesis of uterine fibroids, while Bacteroides, Ruminococcaceae, Roseobacter, Lactobacillus and Firmicutes may be a potential risk factor for the pathogenesis of uterine fibroids. However, more studies are needed to verify the results. This study provides information about the microbiota that can be further studied, which is expected to improve the incidence and prognosis of uterine fibroids.

To evaluate the causal relationships between lipids (low-density lipoprotein cholesterol, high-density lipoprotein cholesterol [HDL-C], triglyceride [TG], apolipoprotein A-I [ApoA-I], apolipoprotein B) and non-alcoholic fatty liver disease (NAFLD) through a two-sample Mendelian randomization (MR) study.

Summary datasets of genome-wide association studies of lipids and NAFLD in European ancestry were leveraged, and single nucleotide polymorphisms associated with lipids were selected as instrumental variables. Several univariable MR analyses were conducted, including inverse-variance weighting method, MR-Egger regression, weighted median estimation, MR pleiotropy residual sum and outlier method. A multivariable inverse-variance weighting method was further used to evaluate the independent causal effect of lipids on NAFLD.

Univariable inverse-variance weighting MR showed that genetically predicted higher HDL-C (OR=0.774, 95%CI: 0.708-0.845, P<0.001) and ApoA-I (OR=0.878, 95%CI: 0.796-0.968, P=0.009) were associated with a lower risk of NAFLD, while genetically predicted higher TG (OR=1.323, 95%CI: 1.201-1.458, P<0.001) was associated with a higher risk of NAFLD. The causal effects of HDL-C and TG on NAFLD remained consistent in multivariable MR when adjusting for other lipids, body mass index, and type-2 diabetes.

Our study supports a putative causal relationship between HDL-C, TG, and NAFLD.

To explore the causal association between gut microbiota and type 2 diabetes mellitus (T2DM) using two-sample Mendelian randomization.

The data of single nucleotide polymorphisms (SNP) of gut microbiota as an exposure factor and type 2 diabetes mellitus as an outcome variable were obtained from the public genome-wide association study database. MR-Egger, weighted median method, inverse variance weighted method (IVW), simple model, and weighted model were used for MR Analysis. The MR-Egger method and IVW method were used for heterogeneity analysis. MR-Egger intercept was used to test the pleiotropy of the data, and the leave-one-out method was used for sensitivity analysis to evaluate the robustness of the results.

A total of 7 gut microbiota were found to be associated with type 2 diabetes in East Asians, and the risk bacteria were Eubacteriumnodatumgroup, Ruminococcaceae UCG010, Lachnospiraceae NK4A136 group, Victivall, and protect bacteria were Enterorhabdus, Erysipelatoclostridium, Romboutsia. There were a total of four kinds of intestinal flora in the European population associated with type 2 diabetes, dangerous bacteria were Howardella, Eubacteriumruminantiumgroup, Eubacteriumventriosumgroup, and protect bacteria were FamilyXIIIAD3011group.

Specific gut microbiota may have causal effects on the risk of type 2 diabetes.

To understand and assess the health risks of five common chemical pollutants, formaldehyde, ammonia, benzene, toluene and xylene, in indoor air of public places in Xining City, and to provide technical support for environmental health management of public places in Xining City.

From 2020 to 2022, monitoring sites were set up in the western and central districts of Xining City to monitor the data of common chemical pollutants in hotels and barbershops, and conduct health risk assessment of chemical pollutants according to the Technical Guide for Environmental Health Risk Assessment of Chemical Substances (WS/T 777-2021).

From 2020 to 2022, there were 238 monitoring points in the hotel, only 2 formaldehyde monitoring points exceeded the standard. The total qualified rate of formaldehyde in the hotel was 99.16%, and the total qualified rate of benzene, toluene and xylene in the hotel was 100%. There are 47 monitoring points in barbershop, and the total pass rate of formaldehyde, benzene, toluene, xylene and ammonia at each monitoring point is 100%. The concentration of benzene in indoor air in barbershop (0.001 mg/m³) was lower than that in hotel (0.002 mg/m³), and the difference was statistically significant (Z=-2.708, P<0.05). Among 47 monitoring points in barbershop, 23 monitoring points had a risk between 10^-4 and 10^-6, accounting for 48.9%, and the rest had a risk value higher than 10^-4; Among 238 monitoring points in hotel, 154 monitoring points had a risk between 10^-4 and 10^-6, accounting for 64.7%, and the rest had a risk value higher than 10^-4; There were 34 monitoring sites in barbershop with risk less than 10^-6, accounting for 72.3%, and 138 monitoring sites in hotel with risk less than 10^-6, accounting for 58%. The risk values of P₇₅ and maximum concentration of both sites were greater than 10^-6. Hotel formaldehyde has 150 monitoring points HQ>1, accounting for 63%, the maximum risk value is 3.6; There were two monitoring points for benzene with HQ>1, accounting for 0.8%, and the maximum risk was 1.08. All HQ of toluene and xylene are less than 1. There were 31 monitoring points for formaldehyde in barbershop, HQ>1, accounting for 66%, and the highest risk value was 3.72. HQ of other pollutants is less than 1.

The concentration of five chemical pollutants in the air of public places in Xining City from 2020 to 2022 basically meets the requirements of the national standard, but the carcinogenic risk and non-carcinogenic risk caused by formaldehyde and benzene should be paid attention to.

To understand the air pollution level, to analyze the source apportionment of metals and metalloids in PM_2.5 and to evaluate its health risk in the city of Hefei.

The concentration of 10 elements (As,Cd,Cr,Ni,Pb, Mn,Se, Al and Sb)in PM_2.5 samples was collected in Hefei city from January 2018 to December 2022, which was used to analyze the pollution level and source apportionment was determined by inductively coupled plasma mass spectrometer(ICP-MS). Correlation analysis, principal components analysis and cluster analysis were used to analyze the sources of metals and metalloids. A health risk assessment model was used to evaluate the health risk of population of 4 carcinogenic elements(As,Cd,Cr,Ni) and 5 non-carcinogenicelements(Pb,Mn,Se,Al,Sb).

The total average concentration of 10 elements in PM_2.5 was in an order of Al>Pb>Mn>As>Se>Sb>Ni>Cr>Cd>Tl and presented an obvious seasonal variation characteristic, higher in winter and spring, lower in summer and autumn.The mixed sources of coal burning, traffic and industry, dust and industrial emissions sources were the main sources of metals and metalloids in ambient PM_2.5 in Hefei.The respiratory pathway exposures of these elements assessed by annual excess risk were between 1.67×10^-7and 4.22×10^-15, which were below the acceptable risk level of population (10^-6).

The main sources of metals in Hefei are mixed sources, soil dust sources and industrial sources. The respiratory pathway exposures of most of the elements assessed by annual excess risk are below the acceptable risk level of population.

To analyze the non-occupational carbon monoxide (CO) poisoning incidents in Jinan City from 2011 to 2022, to grasp their epidemiological characteristics, and propose suggestions for further reducing the morbidity and mortality of non-occupational CO poisoning.

Descriptive epidemiological method was used to analyze the data. The chi-square test was used to compare the rates.

From 2011 to 2022, Jinan City reported 3 003 incidents of non-occupational CO poisoning, with 4 208 cases of poisoning. The average number of poisoning cases per year was 351, with the population over 60 years old being the high-risk group for poisoning. There was a total of 125 deaths, with a fatality rate of 2.97%. The mortality rate of critically ill patients was significantly higher than that of other groups (χ²=45.54, P<0.001). The average annual incidence of non-occupational CO poisoning was 47.48 per million, and the average annual mortality rate was 1.18 per million. The date of occurrence of non-occupational CO poisoning in Jinan City had obvious seasonal characteristics, which was consistent with the winter and spring heating seasons. Among poisoning incidents with clear causes, improper heating was a major cause of poisoning (2 554, 87.4%), followed by gas leaks (207, 7.1%), and the consumption of charcoal hot pots and other charcoal-grilled foods (113, 3.9%).

Although the incidence of non-occupational CO poisoning in Jinan is on a downward trend, the number of people poisoned is still high, and the causes of poisoning are diverse. Therefore it is necessary for multiple departments to jointly carry out the corresponding inspection and preventive work while strengthening the safety education for the residents.

To analyze the status and long-term trends in dietary intake and dietary diversity of adults in Guizhou province from 1991 to 2018.

The food intake was worked out by 3 consecutive days of dietary recollection.The χ² test was used to analyze the dietary intake status of different dietary categories by demographic characteristics in 2018. Cochran-Armitage trend test was used to analyze the trend of the proportion of people at risk. Dietary Diversity Scores (DDS) were conducted based on the intake of 9 food groups, and a general linear model was used to test trends in DDS.

The proportion of insufficient intake of livestock and poultry meat was higher in people aged 60 years or older than in residents aged 18-44 years (χ²=11.931, P<0.001), the differences in the proportions of insufficient/excessive intake of vegetables, fat, oil and salt were statistically significant among ethnic groups (χ² range: 5.852-12.392,P range: <0.001-0.016), and those with higher education had lower proportions of insufficient intake of fruits and livestock (χ² range: 10.173-21.870, P range: <0.001-0.001), people in rural areas had higher excessive proportions of intake of oil and salt and higher insufficient proportions of livestock and poultry meat, fruits and aquatic products, but their proportions of insufficient intake of cereals and potatoes and soya nuts were lower (χ² range: 6.986-39.627, P range: <0.001-0.008) compared with the city and other populations. The proportion of insufficient intake of grains and potatoes, vegetables, soya beans and nuts in the population tended to increase(Z_cereal=17.839,Z _vegetable=11.579,Z_bean&nuts=7.749, P<0.001); the proportion of insufficient intake of fruits, livestock and poultry meat, eggs and dairy products tended to decrease (Z range: -19.991-(-2.764), P range: <0.001-0.006); the proportion of excessive intake of salt and alcohol in the population tended to decrease, while the proportion of excessive intake of sugar tended to increase (Z_salt=-20.899,Z_alcohol=-12.460,Z_sugar=6.791, P<0.001); except for those with education level of tertiary education and above (β=0.005, P=0.520), the Dietary Diversity Score (DDS) of the rest of the subgroups showed an increasing trend (β range: 0.025-0.058, P<0.001).

The dietary structure of residents aged 18 years and above in Guizhou Province is irrational. Although the DDS score has increased, nutritional health interventions should be carried out for different groups of people to improve their dietary quality.

The aim of this study is to test the reliability and validity of the Chinese version of CP-Q among college students.

The English version of the CP-Q was translated, back-translated following the Brislin model, and cultural adaptation was performed to develop the Chinese-version CP-Q.The reliability, validity and feasibility of the questionnaire were evaluated by investigation of questionnaires offline and electronic questionnaires online.

A total of 658 participants were investigated with 593 valid questionnaires obtained, the total effective rate was 90.1%.Subjects finished the Chinese-version CP-Q and Pittsburgh Sleep Quality Index (PSQI); Munich Chronotype Questionnaire (MCTQ) and 24h dietary recalls. Moderate to strong correlations were observed in the sleep/wake schedules between the Chinese-version CP-Q, PSQI and MCTQ, both for work/study days and free days, the highest was the free day sleep time r=0.922(P<0.001).The correlations between sleep, wake, first eating event, lunch time and last eating event in work/study days and free days with the same variables in the 24h recall showed r=0.441-0.659 (P<0.05), in addition to the lunchtime in free days (r=287, P=0.076). A total of 7 items among the 17 variables in the Chinese-version CP-Q evaluated in the test and retest phases presented intraclass correlation coefficients ≥0.75.

The Chinese-version CP-Q has good reliability and validity and could reflect the real situation of eating time and sleeping time. The questionnaire can be used to assess the status of one’s chrononutrition.

To analyze the relationship between childhood emotional abuse (CEA) and short video addiction symptoms(SVAS) in middle school students, and to explore the mediation of cognitive interference(CI) and boredom proneness(BP).

In October 2023, 972 middle school students were tested with childhood emotional abuse scale, short video addiction symptoms scale, cognitive interference scale and boredom proneness scale using the multistage stratified cluster sampling, andprocess was used to analyze chain mediation.

28.19% of short video addiction symptoms was detected, it was higher in girls (χ²=7.961, P=0.005), higher in grade two(χ²=19.747, P<0.001), higher without home visits (χ²=7.221, P=0.007).Total indirect effect of CEA→SVAS was TIE=0.309(95%CI: 0.252-0.374), effect size P_M=69.29%. Indirect effect of CEA→CI→SVAS was IE=0.154 (95%CI:0.113-0.202). Indirect effect of CEA→BP→SVAS was IE=0.079 (95%CI:0.050-0.115). Indirect effect of CEA→CI→BP→SVAS was IE=0.075 (95%CI:0.053-0.104).

CEA can predict SVAS in middle school students through CI and BP.

To understand the epidemiological characteristics and trends of infant deaths due to perinatal diseases in China from 2012 to 2021, in order to provide a reference for the development of related prevention measures and policies.

An epidemiological analysis was conducted on the data of infant deaths due to perinatal diseases from 0-year-old group in the "Chinese Cause of Death Surveillance Dataset" for the years 2012-2021. The average annual percent change (AAPC) in mortality rates was analyzed using the Joinpoint regression model.

The overall mortality rate of infants due to perinatal diseases in China showed a downward trend from 2012 to 2021 (AAPC=-11.89, P<0.001). A declining trend was observed in both male and female infants, as well as urban and rural infants, with AAPCs of -11.73%, -11.98%, -18.70%, and -18.50%, respectively. There was a turning point in the trend for urban infants (APC=-5.20, P=0.124 for 2012-2016 and APC=-18.70, P<0.001 for 2016-2021). The annual average mortality rate due to perinatal diseases was higher in male infants (1.79 per thousand) than in female infants (1.36 per thousand) (χ²=776.474, P<0.001), and higher in rural infants (1.55 per thousand) than in urban infants (0.46 per thousand) (χ²=75.394, P<0.001). The overall mortality rates of "low birth weight, birth trauma and asphyxia, and others" showed a downward trend (AAPCs of -14.10%, -11.10%, and -9.60%, respectively, P<0.001), with differences between urban and rural areas observed in all three perinatal diseases (P<0.001 for each).

The mortality rate of infants due to perinatal diseases in China shows a general downward trend from 2012 to 2021, but the situation of deaths caused by perinatal diseases remains severe. The gap between urban and rural areas is significant, and priority should be given to the perinatal preventive health care of male infants and rural infants.

To investigate the differences in gut flora between infants and children aged 0~3 years with atopic dermatitis (AD) and healthy infants and children.

General information and fecal samples were collected from children with AD aged 0-3 years (AD group) and healthy infants and children (HC group). T Test and Chi-Square Tests were used to analyze the general information of infants and children in two groups, and 16S rRNA sequencing technology combined with bioinformatics analysis to compare the diversity and differences of intestinal flora in two groups.

General information only history of allergy in the immediate family was significantly different between the two groups (χ²=13.875, P<0.001). The difference in the diversity of Gut microbiota between the two groups was not statistically significant. At the gate level, the relative abundance of Verrucomicrobiota was significantly lower in the AD group than in the HC group (P=0.027), whereas the relative abundance of Fusobacteriota was significantly higher than in the HC group (P=0.038). At the genus level, the relative abundance of the genera Cetobacterium (P=0.010), Dysgonomonas (P=0.013), and Fusicatenibacter (P=0.044) in the AD group was significantly higher than that in the HC group, while the relative abundance of the genera Actinomyces (P=0.029), Collinsella (P=0.017) and Eggerthella (P=0.028) were significantly lower in relative abundance than the HC group. LEfSe analysis showed that the relative abundance of Coriobacteriaceae (P=0.017) and Bifidobacterium_longum (P=0.002) in the AD group was significantly lower than that in the HC group.

There are some differences in the composition of the Gut microbiota between the AD and HC groups. Differential microorganisms such as Fusicatenibacter, Eggerthella, Actinomyces and Bifidobacterium_longum may have a correlation with AD development, suggesting that regulating the Gut microbiota early in life may prevent and treat AD.

To explore the incident response capability of adverse event following immunization (AEFI) of vaccination staff in Chongqing.

Descriptive epidemiological methods were used to analyze the level and influencing factors of AEFI response capability among committees and vaccination workers in basic-level CDC and Vaccination units in Chongqing.

Among the 994 surveyed individuals, 97.0% had a medical background. The survey respondents answered AEFI disposal related questions with a total score of 35, with an average score of (18.0±5.1) points. Vaccination personnel with work experience of 21-30 years scored lower(β=-1.446, 95%CI: -2.888--0.004), and the ones who participated in district and county-level events(β= 3.223, 95%CI: 1.597-4.849), participated in municipal level events(β=3.885, 95%CI: 1.882-5.887), as well as city and district/county level participants(β=4.998,95%CI: 3.292-6.705) scored higher.

The disposal ability of AEFI among the basic-level vaccinators in Chongqing is uneven, and training should be strengthened in the future.

To explore the status and influencing factors of health poverty among the elderly in rural areas of Sichuan in the post poverty alleviation era.

By using the method of multi-stage sampling, a questionnaire survey was conducted among 606 rural elderly persons in Sichuan from July to August, 2022. Chi-square test and multivariate logistic regression analysis were used to explore the influencing factors of health poverty.

Among the 606 persons, 167 (27.6%) were in health poverty.The results of multivariate analysis showed that age(OR=1.655, 95%CI: 1.250-2.191), number of chronic diseases (OR=1.864, 95%CI: 1.510-2.301) and physical function limitation (OR=1.890, 95%CI: 1.353-2.640) were the risk factors, while the number of daughters (OR=0.739, 95%CI:0.593-0.920) and labor intensity in the past year (OR=0.498, 95%CI: 0.343-0.722) were protective factors for health poverty in the rural elderly.

The rural elderly in Sichuan still have a high risk of health poverty, especially the elderly who are aged, suffer from multiple chronic diseases, have serious disability, have little labor intensity, and have few daughters are the high-risk groups of health poverty, which needs to be paid attention to.

To understand the impact and effect value of basic medical insurance on the health status of children in China, and to propose corresponding countermeasures and suggestions.

Based on the data from the Chinese Family Panel Studies in 2016 and 2020, this study examined the extent to which basic medical insurance impacts children’s health using the Propensity-Score-Matching and Difference-in-Difference models.

The findings indicated that basic medical insurance could promote the health status of children by 4.9%(β=0.049,SE=0.028). However, the mediating effects of reducing health costs and improving health service utilization were found to be insignificant. Moreover, the study revealed that the health status of preschool children, girls, rural areas, western regions, and low-income families can be improved by 9.1%(β=0.091,SE=0.038), 7.8%(β=0.078,SE=0.043), 9.3%(β=0.093,SE=0.041), 12.0%(β=0.120,SE=0.051), and 9.3%(β=0.093,SE=0.055) respectively.

This study concludes that basic medical insurance plays a positive role in enhancing children’s health. Therefore, it is crucial to continuously strengthen the basic medical insurance system and reinforce the multi-tiered healthcare security framework. Attention should also be given to ensuring fairness for girls and children in rural and western areas, in order to fully protect children’s health.

To explore the relationship between lifestyle and depression symptoms among college students.

A total of 21 143 college students from a private university in Shaanxi province were selected to conduct an online questionnaire survey, including social demographic characteristics, lifestyle and depression symptoms. GAMMA regression and Logistic regression models were used to analyze the association between five lifestyle and unhealthy lifestyle scores and depression symptoms in college students.

The depression symptom score of college students was 29(22,36), and the prevalence of depression symptoms was 15.57%. The prevalence of smoking, drinking, unhealthy diet, lack of physical activity, low body weight or obesity were 17.98%, 24.24%, 83.45%, 80.11% and 46.63%, respectively. The five unhealthy lifestyles were significantly correlated with depression symptoms of college students (all P<0.05). Regression analysis showed that, compared with the unhealthy lifestyle scores of 0-1, the score of depression symptoms increased in the group with 3, 4 and 5 score of unhealthy lifestyle, with β(95%CI) values of 0.57(0.23-0.92), 1.91(1.48-2.34) and 2.47(1.79-3.15), respectively. Compared with the unhealthy lifestyle scores of 0-1, the risk of depression symptoms was increased in unhealthy lifestyle scores of 2, 3, 4 and 5, and the odds ratio value (95%CI) were 1.19(1.02-1.40), 1.41(1.21-1.65), 1.86(1.56-2.21) and 2.18(1.71-2.78), respectively.

Unhealthy lifestyle is related to the increased risk of depression symptoms in college students. The more types of unhealthy lifestyle, the higher the score of depression symptoms and the higher the risk of depression symptoms.

To explore the relationship and mechanism of stress perception and loneliness among emotional intelligence and life satisfaction among master’s students, and to provide reference basis for promoting the mental health development of graduate students.

A total of 728 graduate students from 6 universities were selected by stratified cluster random sampling and investigated by emotional intelligence, loneliness, stress perception and life satisfaction scale. SPSS 25.0 was used to conduct independent sample t-tests, analysis of ANOVA, and Pearson correlation analysis on the data. A structural equation model was bulit by using AMOS 26.0 software and evaluated.

Emotional intelligence was positively correlated with life satisfaction (r=0.423, P<0.01). There was a negative correlation between perceived stress and loneliness, life satisfaction, and emotional intelligence (r=-0.519, -0.440, -0.536, -0.505,P<0.01). There was a positive correlation between perceived stress and loneliness (r=0.568, P<0.01). Emotional intelligence can not only directly predict life satisfaction, but also indirectly through stress perception and loneliness. The mediation effect value of stress perception was 0.227 accounting for 42.5% of the total effect,and the mediation effect value of loneliness was 0.026, accounting for 4.9% of the total effect. The effect value of emotional intelligence and life satisfaction was 0.029, accounting for 5.4% of the total indirect effect.

Emotional intelligence can affect the life satisfaction of master’s students through the mediating or chain mediating effect of stress perception and loneliness, which provides a theoretical basis for improving the quality of life and mental health of master’s students.

To provide a theoretical basis for optimizing and improving the culture medium of Yersinia pestis, the effects of different species and different concentrations of animal blood on the growth of Yersinia pestis were investigated.

Ten kinds of animal blood including rat blood, rabbit blood and guinea pig blood at four concentrations of 0.1 %, 0.5 %, 1 % and 2 % were used as blood stimulators to culture Yersinia pestis in the medium. The culture results were observed and the corresponding statistical analysis was carried out.

The effect of 10 kinds of animal blood on the number of Yersinia pestis colonies at 1 % concentration was different (P=0.015 < 0.05), and pig blood was better than chicken blood (P=0.026 < 0.05). The effect of four concentrations of pig blood on the number of Yersinia pestis colonies was statistically significant (P=0.037 < 0.05), and it was better than the blank at 1 % concentration (P=0.002 < 0.05). The effects of ten kinds of animal blood at four concentrations on the length of Yersinia pestis were statistically significant (P<0.001). The difference in the effect of four concentrations of blood on the length of Yersinia pestis in ten animals was also statistically significant (P<0.001).

When observing the number of colonies, the optimal choice is 1% pig blood. In addition, low concentration and easy-to-obtain blood stimulants can be selected as far as possible. To cultivate a better length of Yersinia pestis, the optimal choice is 0.1% rabbit blood, which can achieve the desired experimental results and save costs. In addition, using the Herxheimer’s sensitive medium with only blood stimulator to culture Yersinia pestis, the characteristics of the strain will not undergo L-type transformation, and the overall characteristics of the strain are stable, which is of great significance for the stable passage and preservation of the strain.

To explore the Rap1/PI3K/AKT signaling pathway in inducing apoptosis of mouse spermatocytes (GC-2 spd) induced by tris (1,3-dichloro-2-propyl) phosphate (TDCIPP)the role of death.

GC-2 spd cells were treated with 0, 15, 30, and 60 μmol/L TDCIPP for 24 hours.Using flow cytometry to detect cell expression levels, Western blotting to examine the expression levels of Rap1, PI3K, AKT, phosphorylated AKT (p-AKT) proteins, as well as related proteins cleared PARP and cleared Caspase-3 in cells, and apoptosis-related proteins cleaved PARP and cleaved Caspase-3 in the cells. ESI09 was used to inhibit the expression of Rap1 protein in GC-2 spd cells, followed by exposure to TDCIPP. Western blotting and flow cytometry were used to respectively assess the protein levels of PI3K, AKT, p-AKT, cleaved PARP, and cleaved Caspase-3, as well as the level of cell apoptosis.

Compared to the control group, the cell apoptosis and expression of cleaved PARP and cleaved Caspase-3 proteins were significantly increased in the TDCIPP-exposed cells (P<0.01). The expression level of Rap1 was significantly increased (P<0.01), while the expression levels of PI3K, AKT, and p-AKT were decreased (P<0.01). Compared to the TDCIPP-exposed group, the expression of Rap1 was down-regulated (P<0.01), while the expression levels of PI3K, AKT, and p-AKT proteins were up-regulated (P<0.05) in the cells co-treated with ESI09 and TDCIPP. Meanwhile, the expression levels of cleaved PARP and cleaved Caspase-3 in the cells, as well as the level of cell apoptosis, were significantly reduced (P<0.05).

TDCIPP induces overexpression of Rap1 protein and obstructs PI3K/AKT signaling transduction, eventually leading to apoptosis of mouse spermatocytes.

To investigate the expression and clinical significance of protein-O-fucosyltransferase 1 in non-small cell lung cancer (NSCLC), so as to provide a reference for the diagnosis and treatment of NSCLC.

The expression of POFUT1in NSCLC was determined by TCGA, UALCAN database and immunohistochemistry. Based on the clinical data form TCGA database were extracted for survival analysis by Kaplan-Meier method, and analysis of the effect of POFUT1 mRNA expression level on prognosis of patients with NSCLC.

POFUT1 was highly expression in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) tissues (all P<0.05). The expression level of POFUT1 mRNA in LUAD was correlated with clinical stage (P<0.001) and that in LUSC was correlated with tumor size (P<0.001). COX regression analysis showed that high mRNA expression of POFUT1 (P=0.012), tumor size, clinical stage and lymph node metastasis were associated with the prognosis of LUAD patients (all P<0.001), and clinical stage III-IV was an independent risk factor for LUAD patients. Tumor size (P=0.001), clinical stage III-IV (P=0.006) and high expression of POFUT1 mRNA (P=0.049) were associated with the prognosis of LUSC patients, and tumor size greater than 5cm and high expression of POFUT1 were independent risk factors for LUSC patients. The survival analysis result showed that the survival rate of patients with high expression of POFUT1 was significantly lower than that of patients with low expression.

POFUT1 is highly expressed in NSCLC tissues, and high POFUT1 expression is correlated with poor prognosis in patients. POFUT1 can serve as a biomarker for prognostic evaluation of NSCLC.

To examine the correlation between body composition and insulin resistance (IR) within the general population of different genders.

The data for this study were sourced from the Hefei area data of the Chinese Residents Cardiovascular Disease and Risk Factors Monitoring Project conducted in 2021, with a total of 1 144 subjects being included,including 568 males and 576 females. The ratio of triglycerides to high-density lipoprotein cholesterol (TG/HDL-C) was used as an alternative indicator of insulin resistance. A chi-square test was employed to analyze the association between body composition metrics—such as body mass index (BMI), percent body fat (PBF), percent body muscle (PBM), visceral fat area (VFG), and waist circumference (WC)—and the TG/HDL-C ratio. Logistic regression analysis was used to identify the factors influencing body composition in individuals with a TG/HDL-C ratio exceeding the tangent point value. The dose-response relationship between body components and the TG/HDL-C ratio was explored using restricted cubic splines, a statistical method that allows for the examination of nonlinear relationships.

The positive detection rate of IR (the rate of TG/HDL-C above the cut point value) was 15.85% in men and 4.69% in women.After adjusting for confounding factors, logistic regression analysis showed that compared with the control group, the risk of IR in the male population was 5.58 times in the obese group (OR=5.58, 95%CI:1.98-15.72), PBF was 7.90 times in the third quartile (OR=7.90, 95%CI:1.68-37.15), the fourth quartile of PBF was 8.20 times (OR=8.20, 95%CI:1.75-38.40), and the third quartile of PBM was 0.27 times (OR=0.27, 95%CI:0.10-0.71), the PBM was 0.09 times in the fourth quartile group (OR=0.09, 95%CI:0.02-0.44), 3.11 times in the group with high VFG (OR=3.11, 95%CI:1.45-6.68), and 4.28 times in the group with higher VFG (OR=4.28, 95%CI:1.45-12.64). Abdominal obesity was 2.21 times (OR=2.21, 95%CI:1.08-4.56). In the female population, the risk of IR was 6.61 times in the obese group (OR=6.61, 95%CI:1.08-65.66) and 8.52 times in the higher VFG group (OR=8.52, 95%CI: 1.90-70.86) (P<0.05). The limited cubic spline showed that BMI, PBF, PBM, VFG, WC had dose-response relationship and approximate linear relationship with TG/HDL-C.

The body composition of community residents is closely associated with the risk of insulin resistance. Individuals who are obese or have high levels of visceral fat are at greater risk for insulin resistance. To mitigate this risk, it is imperative to reinforce lifestyle interventions that prioritize maintaining a higher percentage of muscle mass.

To investigate the influenza vaccination willingness and influencing factors among elderly patients with chronic diseases in Chongqing, providing a scientific basis for the development of targeted vaccine policies and intervention measures.

A multi-stage sampling method was employed to randomly select 1 280 elderly patients with chronic diseases in five districts of Chongqing for an investigation into their influenza vaccination willingness. Univariate analysis was performed using the t-test and chi-square test, and binary logistic regression analysis and the standardized score were used to analyze the influencing factors of influenza vaccination willingness among elderly patients with chronic diseases.

Of the 1 211 eligible survey respondents, only 13.1% of elderly patients with chronic diseases had received the influenza vaccination during the 2022-2023 influenza season, and 56.3% were willing to receive the influenza vaccination in the 2023-2024 influenza season. The willingness to receive influenza vaccination among elderly patients with chronic diseases was higher among ethnic minorities (OR=3.49, 95%CI:1.21-10.02), households or unemployed individuals (OR=2.53, 95%CI:1.05-6.09), individuals with an average monthly household income per capita exceeding 5 000 RMB (OR=2.86, 95%CI:1.33-6.13), those with qualified knowledge of influenza vaccination (OR=1.38, 95%CI:1.02-1.87), and under the policy of free vaccination (OR=56.97, 95% CI:24.38-133.09). The behavioral and social drivers (BeSD) survey indicated that the belief in the effectiveness and safety of the influenza vaccine and fear of infecting friends or family members were the primary driving factors of influenza vaccine uptake, while the perception of the high costs of influenza vaccination was the most significant barrier.

The influenza vaccination willingness among elderly patients with chronic diseases in Chongqing needs further improvement. Governmental authorities, healthcare institutions, and communities should enhance the dissemination of knowledge about influenza vaccines, enhance social support, implement subsidies or free vaccination policies for priority populations, and encourage proactive influenza vaccine uptake among elderly patients with chronic diseases.

To analyse the current situation and trends of wasting and overweight obesity among Han Chinese college students from 2000 to 2019, and to provide a basis for the prevention of wasting and overweight obesity among college students.

The body mass index data of 72 983 Han Chinese college students were selected from five national surveys on students’ physical fitness and health from 2000 to 2019, and the “height-standard weight method” used in the 1985 national survey on physical fitness and health was used to screen for emaciation and overweight-obesity, and the detection rate, increase and annual growth rate of emaciation and overweight-obesity were calculated for college students by gender and age. The detection rate, increase rate and annual growth rate of college students were calculated by gender and age. Statistical analyses were performed using one-way ANOVA, sample-weighted linear regression, χ² test and χ² trend test.

From 2000 to 2019, the BMI of Chinese Han college students all showed a continuous increasing trend, with different growth rates for male and female students in each age group (except for the female group, the P-trend value for male students in each age group was <0.05). Both male and female students' wasting detection rates showed a decreasing trend from 2014-2019.From 2000 to 2019, the overweight and obesity rate of Chinese Han college students continued to increase, and the overweight and obesity rate of male students was higher than that of female students.From 2000 to 2019, the wasting of Chinese Han college students aged 19 to 22 years old The annual growth rate of overweight and obesity of male students has been decreasing, and the annual growth rate of overweight and obesity of male students has been increasing, and the difference in the increase of the annual growth rate of overweight and obesity of male students in each age group is relatively small. 2010-2019, the annual growth rate of overweight and obesity of female students began to increase gradually, and the increase was the largest in 2014-2019, and the increase was the most obvious The largest increase in the annual growth rate of overweight obesity among female students was observed in the age group of 22 years.

At 2019, the trend of the growth of college students' wasting rate has been alleviated, but the overweight and obesity rate is in a continuous growth trend, the relevant government departments should formulate targeted physical health policies to prevent and alleviate the problem of college students’ overweight and obesity.

To analyze the epidemic characteristics of infectious disease public health emergencies in Beijing in recent 10 years, and to provide reference for the prevention and control of infectious disease public health emergencies.

Descriptive epidemiological methods were used to analyze the occurrence characteristics of public health emergencies of infectious diseases in Beijing from 2014 to 2023.

From 2014 to 2023, a total of 443 public health emergencies of infectious diseases were reported in Beijing, with 7 334 cases and 20 deaths. The incidence rate was 1.56% and the fatality rate was 0.27%. There were 240 unclassified incidents (54.18%) and 202 general incidents (45.60%). The main categories were Class B incidents and Class C incidents, with 202 events (45.60%) and 98 events (22.12%) reported respectively. The main transmission routes were life contact transmission, water and food transmission and vector animal and plant transmission, with 192 evens (43.34%), 36 evens (8.13%) and 29 evens (6.55%) reported respectively. The main occurrence places were home and school, with 215 events (48.53%) and 136 events (30.70%) reported respectively. The peak months were June-August and January-February, with 149 events (33.63%) and 105 events (23.70%) reported respectively. The prediction accuracy of the grey model GM(1,1) is excellent, but the difference between the predicted value and the actual value in 2020-2023 is large.

The COVID-19 pandemic has significantly changed the rising trend of public health emergencies of infectious diseases in Beijing, but the prevention and control work in the post-COVID-19 era should not be ignored, and the comprehensive prevention and control of key infectious diseases and imported infectious diseases should be strengthened in key seasons and places.

To analyze the characteristics of suspected adverse events following immunization (AEFI) in Guiyang City from 2014 to 2023, to evaluate the safety of immunization in Guiyang City, and to provide guidance for improving AEFI monitoring.

Collect AEFI data and vaccine immunization data in Guiyang City from 2014 to 2023 through the “National Immunization Planning Information System” and “Guizhou Province Immunization Planning Information Management System.” Use descriptive epidemiological methods to statistically analyze the epidemiological characteristics and reported incidence rates of AEFI in Guiyang City.

From 2014 to 2023, Guiyang City reported a total of 4 498 cases of AEFI, with an average annual reported incidence rate of 14.63 per 100 000. The incidence rate was the highest in 2023 (1 358 cases, 42.43/100 000) and the lowest in 2015 (23 cases, 1.85/100 000). The reported incidence rate had a statistically significant difference among different years (χ²=3 638.04,P<0.001). Yunyan District reported the most cases (1 539 cases, 25.86/100 000), while Xifeng County reported the least cases (86 cases, 6.67/100 000), and the difference among different regions was statistically significant (χ²=890.10,P<0.001). The most cases were reported in November (588 cases, 24.7/100 000) and the least in February (136 cases, 8.37/100 000), and there was a statistically significant difference among different months (χ²=277.31,P<0.001). The male-to-female ratio was 1.10：1.00, with the highest reports in the age group ≤1 year (2 697 cases, 59.96%). The outcome was recovery or improvement in 96.47% of cases.

2014-2023 AEFI in Guiyang is mainly a general response and the vaccination safety is good. However, the work carried out in different places varies and the monitoring sensitivity needs to be further enhanced.

To explore the optimal prediction model for hantavirus hemorrhagic fever with renal syndrome (HFRS) in Hubei province, and to provide a basis for establishing a monitoring and early warning model for HFRS.

Using monthly surveillance data of HFRS incidence in Hubei province from 2005 to 2021, eight single time series models based on exponential smoothing (ETS), seasonal autoregressive integrated moving average (SARIMA) with and without regression variables, a state space model with Box-Cox transformation, ARMA errors, trend, and seasonal components (TBATS), a time series neural network model (NNETAR) with and without regression variables, a linear regression time series model (TSLM), and a cubic spline prediction model (SPLINEF) were used to build 162 models through 1-4 model combinations. The mean absolute percentage error (MAPE) was used as an evaluation index to evaluate the fitting and prediction performance of the models. The comprehensive fitting and prediction performance were evaluated by calculating the mean MAPE of fitting and prediction.

The TSLM model and its combined models had a comprehensive MAPE of more than 100%. Among the other 98 models, the optimal fitting models for single, two, three, and four-model combinations were SPLINEF (11.98%), SARIMA-SPLINEF (15.14%), SARIMA-NNETAR-REG-SPLINEF (16.06%), and SARIMA-TBAT-NNETAR-REG-SPLINEF (17.75%), respectively. The optimal prediction models for single, two, three, and four-model combinations were SARIMA-REG (34.48%), SARIMA-REG-TBATS (22.77%), SARIMA-TBATS-SPLINEF (23.84%), and SARIMA-SARIMA-REG-TBATS-SPLINEF (22.31%), respectively. The optimal fitting and prediction models for single, two, three, and four-model combinations were SPLINEF (24.75%), SARIMA-SPLINEF (22.55%), SARIMA-TBATS-SPLINEF (20.92%), and SARIMA-SARIMA-REG-TBATS-SPLINEF (20.75%), respectively.

Based on the number of models, fitting and prediction accuracy, SARIMA-TBATS-SPLINEF is considered the optimal prediction model and can be used for monitoring and early warning of HFRS in Hubei.

To investigate the interaction between overweight/obesity and dyslipidemia on hyperuricemia (HUA), and to provide scientific basis for the prevention and treatment of HUA.

The data were derived from the physical examination records of workers at the Xishan Coal and Electricity Group in 2018, encompassing a total of 38 615 study participants.SAS 9.3 software was used to conduct χ² test and multivariate logistic regression analyses. Additive interaction model was used to analyze the interaction between overweight/obesity and dyslipidemia on HUA.

The prevalence rate of HUA was 18.28%. After adjusting for gender, age, smoking, drinking, systolic blood pressure, diastolic blood pressure, fasting blood glucose and blood creatinine, the results of multivariate logistic regression analyses showed that overweight/obesity (OR=2.756, 95%CI: 2.584-2.939) and dyslipidemia (OR=2.221, 95%CI: 2.100-2.349) were associated with a higher risk of HUA, respectively. Interaction analyses showed a synergistic additive interaction between overweight/obesity and dyslipidemia on the prevalence of HUA, with those who were overweight/obese and dyslipidemic having a higher risk of HUA than those who had neither (OR=4.840,95%CI: 4.452-5.261), and the relative excess risk of interaction (RERI) was 0.4,the attributable proportion of interaction (API) was 0.083, and the synergy index (SI) was 1.116.

Overweight/obesity and dyslipidemia both increase the risk of HUA, and there is synergistic additive interaction between them.Controlling body mass index and lipid levels may prevent HUA.

To analyze the incidence and mortality of malignant tumors attributed to HPV infection in Sichuan region from 2018 to 2022.

From January 2018 to December 2022, the population, tumor incidence rate and mortality in Sichuan were collected, and the population attributable fraction (PAF) attributable to HPV infection was calculated by age and sex.

The population attributable proportions of HPV infection among residents in Sichuan region from 2018 to 2022 for the incidence and mortality of common malignant tumors were 65.0% and 59.2% respectively. The attribution of HPV (PAF) to the incidence of malignant tumors in the oropharynx, oral cavity, and throat of males was higher than that of females (χ²=78.904, P<0.001).The attributable HPV (PAF) of cervical, vulva, oral, and laryngeal malignant tumors in urban populations was higher than that in rural populations(χ²=119.03, P<0.001).In addition, there was a gender difference (P<0.05) in the relationship between the incidence of tumors attributed to HPV infection and age, indicating that males had earlier onset ages for various tumors. The attribution of HPV (PAF) to death in male oropharyngeal, oral, and laryngeal malignant tumors was higher than that in females(χ²=110.578, P<0.001).The mortality attributable HPV (PAF) of cervical, anal, oropharyngeal, oral, and laryngeal malignant tumors in urban populations was higher than that in rural populations(χ²=66.285, P<0.001).In addition, there was a gender difference (P<0.05) in the relationship between tumor mortality attributed to HPV infection and age, with males dying earlier for oral and oropharyngeal malignancies, and females dying earlier for anal malignancies.

From 2018 to 2022, the incidence and death of tumors attributed to HPV infection in Sichuan region are more, and the incidence and death show an increasing trend, among which timely screening of cervical cancer is the key to reduce the incidence and death of HPV tumors.