To investigate the causal relationship between gut microbiota and insomnia by two-sample Mendelian randomization analysis.

The summary statistics of gut microbiota came from a meta-analysis of the largest genomewide association study conducted by the MiBioGen Alliance. The summary statistics for insomnia came from the genomewide association study of insomnia measurement. Inverse variance weighted, MR-Egger, weighted median, simple mode method, and weighted mode method were used to study the causal relationship between gut microbiota and insomnia. The stability of the results was evaluated by a series of sensitivity analyses.

MR analysis showed that soft membrane Mollicutes RF9 [β=-0.86, OR(95%CI): 0.42(0.25-0.71)] and unknown family [β=-0.86, OR(95%CI): 0.42(0.25-0.71)] had protective effect on insomnia. In addition, 14 other groups were found to be potentially causally associated with insomnia in this study including Oxalobacteraceae (F=170.153, P=0.034), Marvinbryantia (F=162.702, P=0.006), Ruminococcaceae UCG002 (F=298.323, P=0.046), Subdoligranulum (F=198.442, P=0.045), Anaerostipes (F=222.074, P=0.032), Gammaproteobacteria (F=91.816, P= 0.006) , Pasteurellales (F=231.141, P=0.010), Lachnospiraceae UCG010 (F=138.761, P=0.045), Peptococcus (F=287.210, P=0.036), Ruminococcus gnavus group (F=162.913, P=0.031), Dorea (F=112.086, P=0.049), Flavonifractor (F=95.307, P=0.020), and unknown genus(F=89.189, P=0.001). No significant heterogeneity or pleiotropy was detected.

Mendelian randomization shows that the flexurales, unknown families, and 14 other taxa are causally related to insomnia. The gut microbiota may be one of the treatments to improve insomnia.

To explore and compare the application value of post-stroke fatigue (PSF) risk prediction nomogram and Web calculator program in post-stroke fatigue risk prediction in convalescent stroke patients.

The patients with acute stroke admitted to the Department of Encephalopathy of a tertiary hospital from February 2023 to August 2023 were prospectively selected, and the nomogram and Web calculator program were used to predict the risk probability of PSF in convalescent stage. The area under the receiver operating characteristic curve (AUC), Hosmer-Lemeshow goodness-of-fit test, and clinical decision curve were used to evaluate the discrimination, calibration, and clinical utility of the two models, respectively.

A total of 282 patients were included, of which 128 had PSF, and the incidence of PSF was 46.89%. The AUC of the nomogram and Web calculator were 0.687 (95%CI:0.624-0.749) and 0.743 (95%CI: 0.683-0.803), respectively. The Hosmer-Lemeshow goodness-of-fit test showed that the nomogram (χ²=8.357, P=0.213) and Web calculator program (χ²=4.467, P=0.614) were well fitted in stroke patients. The clinical decision curve analysis showed that the nomogram and Web calculator program had clinical benefits in the range of threshold probability of 0.35-0.81 and 0.33-0.88, respectively.

The nomogram and Web calculator program can effectively predict the PSF risk of stroke convalescent patients, and the Web calculator program is superior to the nomogram. However, the future Web calculator program model still needs to be further updated to balance simplicity and accuracy, thereby improving its clinical applicability.

To explore the causal relationship between lifestyle, diet, sleep, socioeconomic status and bronchial asthma by using two-sample Mendelian randomized method.

Two-sample Mendelian randomization analysis was performed based on inverse variance weighting method (IVW), and the stability and reliability of the results were verified by leaving-one-out method, heterogeneity test, and pleiotropy test.

The results of IVW model showed that smoking (OR=1.17,95%CI: 1.08-1.28), drinking (OR=1.22, 95%CI: 1.05-1.41), sedentary behavior (OR=1.78, 95%CI: 1.38-2.31), sleep duration (OR=2.29, 95%CI: 1.38-2.31), and body mass index (OR=1.41, 95%CI: 1.29-1.53) were associated with the increased risk of bronchial asthma. Fresh fruit intake (OR=0.50, 95%CI: 0.34-0.73), dried fruit intake (OR=0.54, 95%CI: 0.34-0.73), cereal intake (OR=0.62, 95%CI: 0.41-0.94), education level (OR=0.57, 95%CI: 0.34-0.73), and social activity (OR=0.26, 95%CI:0.34-0.73) were associated with reduced risk of asthma. The results of MR-Egger intercept detection did not show pleiotropic effects, indicating that Mendelian randomization was an effective method for causal inference in this study. The heterogeneity test results of drinking (Q=139.92, P=0.001), sedentary behavior (Q=151.37, P=0.003), dried fruit intake (Q=40.00, P=0.007), cereal intake (Q=72.95, P=0.001), and body mass index (BMI) (Q=584.12, P < 0.001) were all statistically significant. Therefore, attention was paid to results of the random effect IVW model.

Smoking, drinking, sedentary behavior, sleep duration, and body mass index may be the risk factors of bronchial asthma. Fresh fruit intake, dried fruit intake, cereal intake, education level, and social activities may be the protective factors of bronchial asthma.

Based on the health big data platform of Yichang city, to analyze the characteristics of allergic diseases of children born in medical institutions from 2017 to 2022 in Yichang city, and to clarify the distribution of allergic diseases among children in Yichang city.

Statistical analysis was conducted based on the records of born children with allergic diseases in the outpatient clinic of urban comprehensive medical institutions from 2017 to 2022 in the big data cloud platform of Yichang city.

The number of visits of male children was 68 707, more than that of female children, and the number of visits had been increasing since 2017. The highest number of hospital visits was 46 190 (37.86%) among children of 0-1 year old, of which the highest number of visits was 3 331(13.74%) among children of two months. Atopic dermatitis/eczema and allergic rhinitis had a higher number of visits with 46 156 and 14 388 visits, respectively. The age of onset of atopic dermatitis was earlier, with the age of first consultation being 0.42 years, and the age of onset was earlier in males than in females. Atopic dermatitis/eczema and allergic rhinitis were the most common multimorbidities.

For children with atopic dermatitis/eczema and/or allergic rhinitis, the focus is on the development of concomitant diseases to further interrupt the allergic process and promote early detection, prevention and treatment of the disease.

To evaluate the causal relationship between tea drinking and cholelithiasis by two-way Mendelian randomization.

The instrumental variables for tea drinking were determined from 447 485 participants in the UK Biobank genome-wide association study (GWAS). A total of 32 single nucleotide polymorphisms (SNPs) associated with tea drinking were used for two-sample Mendelian randomized analysis to evaluate the causal relationship between tea drinking and cholelithiasis. Cholelithiasis data were obtained from GWAS data of 35 712 cholelithiasis patients and 273 442 controls publicly available from the Finnegan Alliance. In this study, inverse variance weighted (IVW) was used as the main method to estimate causality, and sensitivity analysis was carried out to ensure the robustness of the results. Finally, reverse Mendelian randomization analysis was used to verify whether there was a reverse correlation.

There was a causal relationship between tea drinking and cholelithiasis. In the IVW method, each additional standard deviation (2.85 cups/day) of tea consumption reduced the risk of cholelithiasis by 26.1% (OR=0.739, 95%CI: 0.536-0.990). Similar results were obtained in weighted mode (OR=0.652, 95%CI: 0.458-0.928), but there was no evidence to prove reverse correlation (IVW: P > 0.05).

The genetic evidence provided by two-way Mendelian randomized analysis shows that the increase of tea drinking reduces the risk of cholelithiasis, but there is no evidence of reverse association.

To construct a deep learning model based on multi-task learning to assist clinicians in differential diagnosis and staging of pneumoconiosis.

The digital chest radiographs of 3 600 patients from an occupational disease hospital in Sichuan Province from 2011 to 2022 were collected, and the full convolution neural network (UNet) was used to segment the lung field. Based on multi-task learning, the multi-task model was constructed using the correlation between tasks.The multi-task model was pre-trained on the ChestX-ray14 dataset, whose backbone network was DenseNet121, and two classifiers were added behind the backbone network. Paired t-test was used to compare the differences in accuracy, precision, sensitivity, and F1 scores between single-task model and multi-task model.

The test set results showed that the differential diagnosis and diagnostic staging performance of the single-task model was about 90% and 77%, respectively. The differential diagnosis and diagnosis staging performance of the multi-task model was about 94% and 86%, which was higher than that of the single-task model about 4% and 9%, respectively. The difference between the evaluation indexes was statistically significant (P < 0.05).

The multi-task model has more advantages than the single-task model and can effectively realize the differential diagnosis and accurate staging of pneumoconiosis and pulmonary tuberculosis.

To investigate the utilization of outpatient and inpatient services in patients with pneumoconiosis and its influencing factors.

By using the convenient sampling method, 199 pneumoconiosis patients were selected from the West China Fourth Hospital, and their population characteristics, medical treatment-seeking behavior, and influencing factors were investigated by using the questionnaire on pneumoconiosis patients and its influencing factors compiled by CDC of China.Multivariate logistic regression model and multivariate poisons regression model were used to analyze the influencing factors of outpatient and inpatient service utilization.

The two-week outpatient rate of pneumoconiosis patients surveyed was 27.6%, the median number of outpatients used in one year was twice, and the annual hospitalization rate was 61.8%. For hospitalized patients, the median number of hospitalizations per year was 1, and the median cumulative hospitalization days was 30 days. The average visual health scale (VAS) score of patients was 48.3±17.4. Multivariate logistic regression and multivariate poisons regression analysis showed that pneumoconiosis patients with basic health insurance (OR=0.067, 95%CI: 0.006-0.716),higher frequency of medication (OR=0.100, 95%CI: 0.037-0.269), and lower VAS score (OR=0.992, 95%CI: 0.988-0.997) had less use of outpatient services. Pneumoconiosis patients with recent exacerbation of cough (OR=5.530, 95%CI: 2.446-12.501), work injury insurance (OR=1.183, 95%CI:1.015-1.379), and lower income (OR=0.980, 95%CI: 0.965-0.997) had more use of outpatient services. Pneumoconiosis patients who were married (OR=0.063, 95%CI: 0.005-0.771) and had lower VAS scores (OR=8.463, 95%CI: 3.090-23.248) had less use of inpatient services. Pneumoconiosis patients with higher frequency of medication (OR=8.463, 95%CI: 3.090-23.248), complicated with core pulmonale (OR=1.855, 95%CI: 1.307-2.634), pneumoconiosis compensation (OR=3.358, 95%CI: 1.183-9.529), social relief (OR=1.402, 95%CI: 1.047-1.877) and more active communication with patients (OR=2.158, 95%CI: 1.061-4.390) were likely to have more use of inpatient services.

The utilization rates of outpatients and inpatients with pneumoconiosis are higher than the national average. The health status, economic level, and social support level of patients with pneumoconiosis are important factors affecting their medical treatment behavior.

To explore the pollution characteristics and ecological risk of microplastics in Kundulun Reservoir in Baotou city.

Three environmental samples were collected from the surface water, sediment, and surrounding soil of Kundulun Reservoir. After four steps of digestion, density flotation, secondary digestion and concentration, the microplastics were extracted. The abundance, polymer type, particle size, and shape of microplastics were detected by LDIR8700 reflection model controlled by Clarity software, and the ecological risk was assessed by risk index (H) and pollution load index (PLI).

The average abundance of microplastics in surface water, sediment, and surrounding soil was (210.83±50.1) n/L,(9 633.33±1 955.33) n/kg, and (10 633.33±896.29) n/kg, respectively. In the surface water, the main type of microplastic polymer was polypropylene (PP), the microplastic with the particle size of 0 to 30 μm accounted for the largest proportion, and the shape was mainly in the form of film. In sediments, polybutylene succinate (PBS) was the main type of polymer, and the size of 0 to 30 μm and film-like microplastics were the main types. In the surrounding soil, the main type of polymer was PBS, and the particle size and shape of microplastics were the same as those in sediments. The risk index (H) based on the type of microplastic polymer showed that the microplastic H index of each sampling site ranged from 1.22 to 9 111.44, and the risk grade covered grades Ⅰ to Ⅳ. The risk grade in surface water was generally high, and the risk grade in sediment and surrounding soil was slightly lower. The PLI based on the pollution abundance of microplastics showed that the range of PLI index of microplastics in each sampling site was 1: 1.2733, and the average PLI index of microplastics was 1.0995, which showed slight pollution in the three environmental media.

The abundance of microplastics in the three environmental media of Kundulun Reservoir is high, with a wide range of sources and mainly small particle sizes. The overall risk index is relatively high, and the pollution load is low.

To comprehensively evaluate the technical support ability of occupational disease surveillance and evaluation of the county-level Center for Disease Control and Prevention (CDC) in Sichuan Province, in order to optimize the allocation of resources, improve the support ability of occupational disease prevention and control, and provide reference for balanced development.

The technical support ability of occupational disease surveillance and evaluation of county-level CDCs in Sichuan Province was comprehensively evaluated by entropy weight TOPSIS method, followed by classification by RSR method.

Among the 179 county-level CDCs in Sichuan Province, 29 county-level CDCs such as Jiangyou, Mianzhu, and Zhongjiang were rated as “good”, and 28 county-level CDCs such as Shiqu, Batang and Daocheng were rated as “poor”.The rest were rated as “medium”. Kruskal-Wallis H test showed that the grading results were statistically significant (H=120.21, P < 0.001).

The overall level of technical support ability of occupational disease surveillance and evaluation of county-level CDCs in Sichuan Province is not high, and there are great differences among economic zones and within cities (prefectures). In order to achieve an overall improvement and balanced development of technical support capacity for occupational disease monitoring and assessment in county-level CDCs in Sichuan Province, more policy attention should be given, occupational and radiological health technology services certification should be vigorously promoted, targeted training and professional title promotion should be strengthened for specialized talents, and critical instruments should be gradually equipped.

To analyze and summarize the relationship between sleep quality and dietary inflammatory index (DII), so as to provide guidance for improving sleep quality.

Using dietary inflammatory index, anti-inflammatory diet, anti-inflammatory diet, and sleep quality as keywords, China knowledge net, VIP database, Wan fang database, PubMed and Web of Science database were searched, and the related literatures at home and abroad were reviewed to summarize the relationship between DII and sleep quality and its internal mechanism.

There was a correlation between sleep quality and DII, but the conclusion was not consistent. On one hand, pro-inflammatory or anti-inflammatory diet may affect sleep quality by affecting circadian rhythm system and sleep homeostasis. On the other hand, low-quality sleep may affect dietary selection and intake, thus affecting the level of dietary inflammation.

There is a correlation between sleep quality and DII, but there is no consistent conclusion. Larger samples and high-quality studies are needed in order to provide reference for improving sleep quality.

To investigate the relationship between the intake of vegetables and fruits and the composition and diversity ofgut microbiota during the first trimester of pregnancy in Chengdu.

In total 104 early pregnant women in Chengdu were enrolled in this study. Dietary frequency questionnaire was used to collect the intake of vegetables and fruits. According to the median intake of vegetables and fruits, the early pregnant women were divided into high intake group and low intake group. The intestinal microflora of fecal samples of early pregnant women was detected by 16S rRNA highthroughput sequencing, and the differences of intestinal microflora between groups were compared. Multiple linear regression was used to explore the relationship between vegetable and fruit intake and intestinal flora in early pregnant women.

The median intake of vegetables and fruits in early pregnant women was 90.0 g/d and 200.0 g/d, respectively. There was no significant difference in intestinal microflora α and β diversity between high and low intake of vegetables and fruits in early pregnancy. LEfSe analysis showed that the relative abundance of multiple intestinal microflorae in early pregnant women was significantly different between the groups with high and low intake of vegetables and fruits. Multiple linear regression analysis showed that after adjusting age, pre-pregnancy body mass index, and energy intake, early pregnant women’s vegetable intake was directly proportional to the relative abundance of Eubacterium siraeum group (β=0.007, P=0.012), inversely proportional to the relative abundance of Bacteroides (β=-0.167, P=0.023), and fruit intake was proportional to the relative abundance of Ruminococcus gnavus group (β=0.004, P=0.034), inversely proportional to the relative abundance of Agathobacter (β=0.004, P=0.044).

The intake of vegetables and fruits in early pregnancy leads to the change of relative abundance of intestinal flora. This study provides a new scientific basis for dietary regulation of gut microbiota during pregnancy.

To investigate the influence of income level on fertility intention of migrant women in China, and to provide theoretical support for the formulation and implementation of supporting measures to enhance the fertility intention of migrant women.

Based on the survey data of health and family planning of floating population in 2018, the influence of income level on fertility intention of migrant women was analyzed by ologist regression model, and robustness test and heterogeneity test were carried out.

The results of ologist model regression analysis showed that the income level had a significant positive effect on the fertility intention of migrant women (OR=1.182, 95%CI: 1.096-1.274, P < 0.01). The robustness test and heterogeneity test showd that the positive effect of income level remained stable.

Migrant women with higher income levels have higher fertility intention, and income levels have a positive effect on the fertility intention of migrant women.This suggests that a variety of measures including raising the income level on all fronts could be taken to enhance the fertility intention of migrant women.

To understand the prevalence and annual change trend of Internet addiction among students aged 11 and 22 in Inner Mongolia in 2019 and 2021, and to explore the influencing factors of Internet addiction behavior, so as to provide reference basis for the prevention of Internet addiction among students.

Students were selected from 12 cities of Inner Mongolia Autonomous Region by stratified cluster random sampling. Internet addiction disorder was determined according to the items of “surveillance and intervention of common diseases and health influencing factors of students in China”. Logistic regression method was used to analyze the relationship between Internet addiction and behavioral factors.

The prevalence of Internet addiction among students aged 11 to 22 years in Inner Mongolia in 2019 was 1.92%, 2.50%, and 2.21%, respectively. Logistic regression analysis showed that vocational high school (OR=1.347, 95%CI: 1.083-1.675), single parent family (OR=1.279, 95%CI:1.090-1.500), reconstituted family (OR=1.326, 95%CI: 1.041-1.690), bullying (OR=1.755, 95%CI: 1.443-2.135), depression (OR=3.557, 95%CI: 3.146-4.021), fighting (OR=3.374, 95%CI: 2.512-4.531), sugary drinks (OR=2.182. 95%CI:1.753-2.716),and eating fried food (OR=2.398, 95%CI: 1.908-3.014) were the risk factor of Internet addiction. Outdoor activities for 2 hours (OR=0.620, 95%CI: 0.530-0.726), non-smoking (OR=0.553, 95%CI: 0.476-0.643), and non-drinking (OR=0.581, 95%CI:0.507-0.666) were the protective factors of Internet addiction.

The current situation of Internet addiction among students in Inner Mongolia is not optimistic. Attention should be paid to bullying, depression, reconstituted families, fighting students, and vocational high school students. Outdoor activities for 1 to 2 hours a day, reducing the intake of sugary drinks and fried foods, and no smoking and drinking can effectively reduce the incidence of Internet addiction.

To analyze the status of non-response to hepatitis B vaccine and its related factors in children born to mothers infected with hepatitis B virus (HBV).

The children born to mothers with HBV infection reported in Hunan Province from January to December 2022 were selected. All the children received hepatitis B immunoglobulin injection and 3 doses of hepatitis B vaccination and were followed up to 7-12 months of age for detection of hepatitis B surface antigen (HBsAg) and anti-HBs. Both HBsAg and anti-HBs were negative, indicating that there was no response to hepatitis B vaccine in children. Unconditional binary logistic regression model was used to analyze the related factors of non-response to hepatitis B vaccine in children.

A total of 9 792 children born to mothers infected with HBV were analyzed. Among 9 792 children, the non-response rate of hepatitis B vaccine was 9.5% (928/9 792). Non-conditional binary Logistic regression analysis showed that the risk of non-response to hepatitis B vaccine in children born to HBeAg-positive mothers was 1.62 times higher than that in children born to HBeAg-negative mothers (OR=1.62, 95%CI: 1.40-1.88). The risk of non-response to hepatitis B vaccine in children with macrosomia (birth weight ≥ 4 000g) was 1.41 times higher than that in children with normal birth weight (OR=1.41, 95%CI: 1.08-1.85). Maternal antiviral therapy (OR=0.59, 95%CI: 0.48-0.73) and child HepB standard vaccination (OR=0.66, 95%CI: 0.57-0.78) were the protective factors of non-response to hepatitis B vaccine in children.

The non-response rate of hepatitis B vaccine in children born to HBV infected mothers is high, and it is affected by maternal HBeAg positive, antiviral therapy during pregnancy, birth weight, and standard vaccination.

To analyze the mediating effect of sleep duration and sleep quality on physical activity level and cognitive function of elderly residents in rural areas of Chengdu.

By using the method of multi-stage random sampling, subjects over 60 years old were recruited in rural areas of Chengdu in October 2020. A total of 732 subjects were included in this study.A questionnaire survey was conducted by using the physical activity scale for the elderly and telephone interview questionnaire on cognitive status. Linear regression model was used to analyze the effect of physical activity level on cognitive function, and intermediary software package (mediation package) was used to explore the mediating effect of sleep duration and sleep quality.

Physical activity had a positive effect on cognitive function (β=0.004, P<0.0l), and there was a partial mediating effect of sleep duration in the relationship between physical activity and cognitive function in sleep duration (P<0.05). The mediating effect was 0.000 4, accounting for 7.74% of the total effect (0.004 1). There was a partial mediating effect of sleep quality between physical activity and cognitive function (P<0.01). The mediating effect was 0.000 9, accounting for 19.18% of the total effect (0.003 6).

In order to prevent and delay the decline of cognitive function, elderly rural residents should continue to participate in physical activities, and it is necessary to carry out intervention programs aimed at their sleep duration and sleep quality to promote the improvement of their cognitive function.

To analyze the resource allocation efficiency and spatial aggregation of elderly care institutions in China, so as to provide reference for optimizing the elderly care service system.

Three-stage DEA model was used to analyze the resource allocation efficiency of elderly care institutions in 31 provinces (autonomous regions and cities) in 2021. Global Moran’s I index and Getis-Ord Gi* index was used to analyze the spatial autocorrelation of resource allocation efficiency of elderly care institutions in China.

In 2021, the overall scale efficiency of resource allocation of elderly care institutions in China was not high, and there was spatial aggregation effect. Scale efficiency was the main factor restricting the improvement of the allocation efficiency of elderly care institutions in China. The resource allocation efficiency of elderly care institutions was greatly affected by the external environment.

According to the demand of local elderly care service, the government should adjust its scale efficiency reasonably, improve the management level, allocate the resources of elderly care institutions reasonably and promote its efficiency.

To explore the current situation and development trend of coupling coordination between medical and health resource allocation and service utilization in eastern, central, and western regions and provinces of China, and to provide suggestions for promoting the coordinated development of medical and health resource allocation and service utilization.

The related index data of medical and health resource allocation and service utilization from 2018 to 2022 were selected, and the comprehensive development level index and relative development degree were used to evaluate the medical and health resource allocation and service utilization. The coupled coordination degree model was used to analyze the coupling coordination relationship and development trend of the two systems.

There were significant differences in the comprehensive development level of medical and health resource allocation among provinces, and the overall structure of relative development degree in 2021 was “9-11-11”. From 2017 to 2021, the coupling coordination degree of medical and health resource allocation and service utilization decreased, showing a decreasing trend from east to west as a whole. The overall coordination between the allocation of medical and health resources and the utilization of services in China was poor. Only 12 provinces achieved coordinated development in 2021. Xizang was still in the dilemma of serious imbalance.

It is suggested to improve the accuracy of medical and health resources allocation, reduce the differences in the development level of resource allocation between provinces, and build a multi-party co-governance pattern. The improvement strategy of coordination level should be accurately formulated according to the relative development of medical and health resource allocation and service utilization in each province.

To analyze the coupling and coordination relationship between the supply of medical resources and the utilization of medical services in Chengdu-Chongqing economic circle from 2016 to 2021, so as to provide reference for optimizing the allocation of medical resources.

Based on the comprehensive evaluation index system of medical resources supply and service utilization, a comprehensive evaluation model and a coupling coordination degree model were constructed to analyze the coupling and coordination relationship between medical resources supply and service utilization in Chengdu-Chongqing economic circle from 2016 to 2021. With the use of exploratory spatial data analysis, the spatial-temporal evolution characteristics of medical resources coupling coordination were analyzed.

From 2016 to 2021, the development levels of medical resources supply and service utilization in Chengdu-Chongqing economic circle were 0.11-0.13 and 0.14-0.15, respectively, and the relative degree of development changed from shortage of medical resources (0.78) to mutual complement and promotion of development (0.92). However, the proportion of cities subject to lagging supply of medical resources was still large (68.8% in 2021). The level of coupling coordination changed from 0.47 to barely coordinated development. By 2021, only Chongqing and Chengdu had a good coordinated development. The LISA diagram showed that the “high-high” areas increased year by year, and the “cold point” effect disappeared, but there was still a “highland” phenomenon centered on Nanchong.

The development level of medical resource supply and service utilization in Chengdu-Chongqing economic circle is stable and rising, but the overall level is low and the regional gap is obvious. The coupling coordination level needs to be further improved, which is mainly limited by the lagging supply of medical resources. The spatial positive correlation of coupling coordination degree is gradually enhanced, but the spatial heterogeneity is still prominent.

To Chinesize the Nursing Home Care-related Quality of Life Scale (NHC-QoL) and to test its reliability and validity.

According to the Brislin model, the NHC-QoL scale was translated into Chinese and cross-cultural debugging was carried out. Using convenience sampling method, 198 elderly people from 4 nursing institutions in Shiyan city were investigated by questionnaire from March to July 2023 to evaluate the reliability and validity of the scale.

There were 14 items in the Chinese version of NHC-QoL. The content validity index of each item was 0.7-1.0, and the content validity index of the scale was 0.929. Exploratory factor analysis extracted 5 common factors, which could explain 67.914% of the total variation.After discussion within the research group, the common factors were reduced to 2 common factors namely environment and service (11 items) and social interaction (3 items). The total Cronbach’s α coefficient of the scale was 0.758 and the test-retest reliability was 0.783.

The Chinese version of NHC-QoL scale has good reliability and validity and can be used to evaluate the quality of life of the elderly in nursing institutions.

To explore the mutual predictive relationship between mobile social media Fear of Missing Out (FoMO) and Mobile Phone Addiction (MPA) among vocational college students, to provide scientific reference for the prevention and education of college students’ mobile phone addiction behavior.

With the use of cluster random sampling, 1 000 college students from a vocational college in Shenzhen were investigated by mobile social media FoMO scale and MPA scale in September 2021 (T1) and December 2021 (T2). The data were analyzed by cross-lag regression analysis.

There was a significant positive correlation including the synchronous correlation and the consecutive correlation between mobile social media FoMO and MPA (P<0.05). Mobile social media FoMO T1 positively predicted MPA T2 (β=0.25, P<0.001), whereas MPA T1 did not predict mobile social media FoMO T2 (β=-0.01, P=0.76). Three dimensions of mobile social media FoMO could predict MPA synchronously (P<0.05) and behavioral performance T1 could predict MPA T2 consecutively (P<0.05). Two dimensions of MPA,loss of control and avoidance, could predict mobile social media FoMO synchronously (P<0.05), and avoidance T1 could predict mobile social media FoMO T2 consecutively (P<0.05).

Vocational college students with higher FoMO are more likely to have MPA. There is a one-way predictive relationship between mobile social media FoMO and MPA.

To understand the current situation and characteristics of chronic disease comorbidities among the elderly living alone in China, to analyze their comorbidity patterns, and to explore the relationship between health-related behaviors and chronic comorbidity in the elderly living alone, so as to provide basis for co-prevention and co-management of multiple diseases in the elderly.

Based on the data of the 2018 Chinese longitudinal healthy longevity survey (CLHLS), the network map was used to identify the common binary co-disease patterns of chronic diseases in the elderly living alone, and the Gephi software was used to visualize the chronic disease co-disease network of the elderly. Using Apriorism algorithm, the association rules were used to analyze the correlation between health-related behaviors and co-diseases of the elderly living alone, and multi-factor Logistic regression model was used to analyze the correlation between chronic diseases and health-related behaviors of the elderly living alone.

A total of 1 905 elderly people living alone over 65 years were included, of whom 766 suffered from two or more chronic diseases, and the co-morbidity of chronic diseases was 40.2%. A total of 45 meaningful binary co-disease patterns with strong links were identified through network map analysis, of which 10 were related to hypertension and 7 were related to heart disease. The strong association rules among the association rules of health-related behaviors and co-diseases of the elderly living alone were poor sleep quality, no physical activity, no exercise, and no outdoor activity. The multivariate Logistic regression model showed that the elderly living alone with smoking (OR=1.791, 95%CI: 1.205-2.664) and alcohol consumption (OR=1.597, 95%CI: 1.084-2.353) had an increased risk of chronic co-disease compared with those without chronic diseases. Elderly people living alone with exercise (OR=0.132, 95%CI: 0.100-0.175), outdoor activities (OR=0.047,95%CI: 0.035-0.063), good sleep quality (OR=0.469, 95%CI: 0.319-0.688), and adequate sleep (OR=0.648, 95%CI: 0.484-0.867) had a lower risk of chronic comorbidity.

The comorbidity pattern of the elderly living alone in China is complex and related to health-related behaviors. The risk of comorbidity in the elderly can be reduced by improving health-related behaviors, such as increasing exercise and outdoor activities and adjusting sleep duration and sleep quality.

To explore the relationship among cognitive function, anxiety, and self-management behavior in elderly patients with diabetes mellitus.

A face-to-face survey was conducted among elderly patients with type 2 diabetes mellitus aged 60 and over in the Department of Endocrinology, Penglai People’s Hospital, Yantai city, Shandong Province from October 2021 to May 2022. The Geriatric Anxiety Inventory-Short Form (GAI-SF), the Montreal Cognitive Assessment (MoCA),and self-designed items were used to assess the anxiety, cognitive function, and self-management behaviors of the patients.Path analysis and mediation effect testing were conducted using a structural equation model.

In total 1 319 diabetic patients aged 60 and above were enrolled, of whom 8.3% had anxiety symptoms. The average scores of MoCA scale and self-management behavior were (23.89±4.12) and (3.74±1.27), respectively. Structural equation model analysis showed that cognitive function had a masking effect between anxiety and self-management behavior, and anxiety had a positive effect on self-management behavior directly (β=0.127, P < 0.019). However, it also had a strong negative effect on cognitive function (β=-0.567, P < 0.001) and then had a negative effect on self-management behavior (β=-0.241, P < 0.001). Therefore, anxiety had a negative effect on self-management behavior (β=-0.115, P < 0.001).

The anxiety of elderly patients with diabetes is negatively correlated with their self-management behavior and can have a negative effect on self-management through cognitive function.

To understand the current status of knowledge and willingness of influenza vaccination among the unvaccinated elderly people in a southwestern city, and to analyze the factors affecting the willingness of influenza vaccination, so as to provide a basis for promoting the popularization of influenza vaccine.

A total of 600 elderly people aged 60 and above who were not vaccinated with influenza vaccine from October to December 2023 were surveyed on their knowledge and willingness to be vaccinated with influenza vaccine. Finally, 538 valid questionnaires were collected. Univariate analysis was carried out using t-test and χ² test, and logistic regression model was used to analyze influenza vaccination intention.

In total 57.8% of the 538 elderly people were willing to be vaccinated against influenza. Specifically, 55.6% of the elderly “did not know or have not heard of the influenza vaccine”, 53.2% of the elderly believed that “the influenza vaccine may not be effective”, 67.5% of the elderly believed that “influenza vaccination would have side effects”, and the estimated proportion of side effects was at 17.53%. The results of multivariate analysis of influenza vaccination willingness showed that people who had “general knowledge” (OR=2.464, 95%CI: 1.209-5.019, P=0.013) and who were “very knowledgeable” (OR=2.885, 95%CI: 1.112-7.485, P=0.029) were more likely to be vaccinated than those who had not heard of influenza vaccine.Compared with the “estimated proportion of influenza vaccination in people aged 60 and over < 20%” group, the “estimated vaccination ratio of 60% to 80%” group (OR=3.478, 95%CI=1.555-7.779, P=0.002), and the “estimated vaccination ratio ≥80%” group (OR=4.591, 95%CI=1.058-19.915, P=0.042) had higher willingness to vaccinate influenza vaccine. The higher the score of satisfaction with personal health, the lower the willingness to be vaccinated against influenza (OR=0.804, 95%CI=0.670-0.964, P=0.018).

The elderly people who have not been vaccinated with influenza vaccine in this area do not know enough about influenza and its vaccine, and the willingness of influenza vaccination is low, which needs to be improved urgently. It is suggested that the knowledge and publicity of influenza and its vaccine be strengthened in this region, and practical vaccination strategies should be adopted to improve the willingness and vaccination rate of influenza vaccination.

To investigate the epidemiological characteristics and pathogen distribution of burn patients at the hospital.

The medical records of 2 434 burn patients who met the inclusion criteria in the First Affiliated Hospital of Anhui Medical University from 2019 to 2022 were analyzed, including sex, age, injury factors, burn site, burn severity, and seasonal distribution of burns, as well as the type of pathogen of infection, the source of tissue/body fluid and its drug resistance. The species of pathogens were identified by Matter-assisted Laser Desorption Time-of-flight Mass Spectrometry (VITEK-MS), and the drug sensitivity of the top three pathogens was determined by VITEK2 Compact. SPSS27.0 and WHONET5.6 statistical software were used to analyze the data.

A total of 2 434 burn patients were enrolled in this study, with a male-to-female ratio of 2.2915. The average age was 29.8 ±25.95 years old. Adults and the elderly were most likely to have burns in summer, while children were more likely to suffer from burns in spring. Hot water scald was the main cause of disease. A total of 952 strains of pathogenic bacteria were collected, all of which were isolated for the first time. The top five pathogens were Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Klebsiella pneumoniae, and Enterobacter cloacae. The number of pathogens isolated from wound secretions was the largest, accounting for 83.93% (799/952). The resistance rate of Pseudomonas aeruginosa to the first and second generation cephalosporins was 100%, but the resistance rate to aztreonam, tobramycin,and piperacillin decreased. The resistance rates of Acinetobacter baumannii to polymyxin B, minocycline, and tigecyclines were all 0, and the resistance rates to other antibiotics were all ≥ 50.0%. The resistance rate to penicillin and Staphylococcus aureus was more than 90%, but it was completely sensitive to vancomycin, linezolid, tigecyclines, and ampicillin/sulbactam.

Based on the age range division, there are significant differences in gender, cause of injury, prehospital treatment, burn severity, and burn site. Scald is the main cause of injury, limbs are the most prone to burn, and cases occur in summer most commonly. The main pathogen of burn infection is Pseudomonas aeruginosa, which mainly comes from wound secretions.

To analyze the transmission of HIV-1 infections newly found in Luzhou city, Sichuan Province in 2021,and to investigate the risk factors and characteristics of HIV-1 transmission network, so as to provide data for the prevention and control of AIDS epidemic in Luzhou.

The blood samples and demographic information of 363 newly reported HIV-1 infections in Luzhou in 2021 were collected. The HIV-1 pol gene sequence was amplified by nested PCR, and the virus subtype and transmissible drug resistance were analyzed according to the pol gene sequence. The molecular network was constructed based on the optimal gene distance (d=1.5%) and the characteristics of molecular transmission network and its influencing factors were analyzed.

A total of 346 valid sequences were obtained, including 113 cases of CRF07_BC subtype (32.66%),107 cases of CRF01_AE subtype (30.92%), 99 cases of CRF08_BC subtype (28.61%), 18 cases of CRF105_0108 subtype (5.20%), and 9 cases of other subtypes (2.60%). The rate of transmitted drug resistance of HIV-1 was 15.03% (52/346), and dual drug resistance was detected in 4 samples. A total of 34 molecular clusters were formed, and the number of cases in the clusters ranged from 2 to 25, with 148 individuals (42.77%) included in the transmission network under the optimal genetic distance threshold (1.5%). There were significant differences in age, education level, infection route, genotype, and registered residence registration (P<0.05). Logistic regression found that education level and registered residence were the influencing factors of network access.

The newly reported HIV-1 infection in Luzhou city shows diversity of virus genes. CRF01_AE,CRF07_BC, and CRF08_ BC subtype have become the main subtype of epidemic virus strains, and molecular transmission network should be established for long-term monitoring in this region to take targeted measures to block its further transmission.

To analyze the basic hygiene status and main health hazard factors of public places in Hainan Province, so as to provide technical support for promoting health management of public places.

The health hazard factors of 200 public places in Hainan Province were monitored in 2022, and the results were statistically analyzed.

A total of 2 339 samples from public places in Hainan Province were monitored, and the overall qualified rate was 83.9%. There were significant differences in the qualified rates of samples from different types of places (χ²=120.63, P < 0.05). Among them, the qualified rates of swimming pools (halls) and hotels (hotels) were relatively low, which were 65.8% and 76.3%, respectively, while the overall qualified rates of the other five types of places were between 85.7% and 96.5%. The main unqualified indexes of indoor air in hotels were formaldehyde, the total number of bacteria, PM₁₀, and PM_2.5. The main unqualified indexes of indoor air in barber shops and beauty shops were ammonia and PM₁₀. The main unqualified index of indoor air in shopping malls (supermarkets) was the total number of bacteria. The total number of bacteria detected in all kinds of public goods and appliances in 6 types of public places exceeded the limit. The unqualified rate of pH value of towels in hotels, beauty shops, and barber shops was 43.2%, 13.6% and 11.4%, respectively. The main unqualified indexes of swimming pool were the pH value of swimming pool water, urea and oxidation-reduction potential, and the unqualified rate of oxidation-reduction potential was 29.6%.

The hygienic condition of public places in Hainan Province still needs to be improved in 2022. It is suggested that the relevant departments further strengthen the supervision and monitoring of the total number of bacteria, ammonia, PM₁₀, PM_2.5 and formaldehyde in indoor air, the total number of bacteria, coliform group and pH value of towels for public goods, and the redox potential, urea and pH value of swimming pool water.

To understand the detection of four prohibited veterinary drugs of fresh and live freshwater fish sold in Guangzhou by analyzing the test data from risk monitoring and supervision sampling, so as to provide reference basis for food safety supervision.

Through the Guangzhou Food Safety Risk Monitoring system and the Food Safety Supervision and Inspection Public Information report of Guangzhou Market Administration, the test results of fresh water fish samples collected from supermarkets, markets, and online e-commerce in Guangzhou from 2017 to 2021 were obtained. The detection of nitrofuran drugs, chloramphenicol, sodium pentachloro phenate, and malachite green drugs were analyzed.

The total detection rate of banned veterinary drugs in risk monitoring samples was 35.3% (84/238), and that in supervised sampling samples was 3.10% (118/3 806). The banned veterinary drugs with high detection rates of the two data sources were different. The risk monitoring rates of chloramphenicol and sodium pentachloro phenate were 17.23% (41/238) and 17.23% (41/238), respectively, and that of malachite green was 2.18% (83/3 806). The drug detection rate of the samples collected by risk monitoring in the network e-commerce link was 47.50% (19/40), which was the highest among all sampling links. The results of the two data sources showed that the detection rate of banned veterinary drugs in the wholesale market was high: 47.37% (18/38) and 4.13% (5/121),respectively.

The results of data analysis from the two sources complement each other and jointly reveal the problem of banned veterinary drug residues in fresh freshwater fish sold in Guangzhou from 2017 to 2021. It is suggested that the relevant departments pay more attention to the illegal use of chloramphenicol, sodium pentachloro phenate, and malachite green drugs.

To evaluate the potential causal relationship between blood metabolites and the risk of heart failure by two-sample Mendelian randomization.

Based on a genome-wide association study containing 486 blood metabolites, single nucleotide polymorphisms (SNPs) independently associated with blood metabolites was selected as instrumental variables. The heart failure data were derived from the Genome-Wide Association Study of the Molecular Epidemiology Research Consortium for Therapeutic Targets in Heart Failure. The potential causal relationship between them was analyzed by inverse variance weighting method, MR-Egger regression method, and weighted median method, and the sensitivity analysis was perfomed using MR-Egger regression intercept, Cochran Q test, MR-PRESSO, and leave-one-out method.

In total 7 516 SNP associated with blood metabolites were selected as instrumental variables. The results of inverse variance weighting analysis showed that pantothenic acid was associated with heart failure (OR=1.29, 95%CI: 1.03-1.63). Sensitivity analysis results indicated that the results were robust.

This study suggests that there is a positive causal relationship between pantothenic acid and the risk of heart failure, which may provide new insights into the pathogenesis and drug targets of heart failure.

Tumor is a major public health problem harmful to people’s health. Cell copper death provides a new idea for the basic research of tumor. This paper reviews the latest progress of cell copper death in the field of tumor prevention and treatment.

The literatures related to copper death in tumor prevention and treatment were summarized, and the research status of copper death related genes in different types of tumors was reviewed.

Ferredoxin 1 (FDX1), lipoic acid synthase (LIAS), dihydro lipocyte answers (DLAT), and dihydro lipoamide S-succinyl transferase (DLST) were the key regulatory genes of copper death, which were abnormally expressed in lung cancer, hepatocellular carcinoma, and breast cancer. Among them, the low expression of FDX1 in tumor patients was related to the poor prognosis of the disease, while the overall survival time of cancer patients with high expression of DLAT and DLST genes was decreased. In addition, targeting copper death protein and promoting cell copper death inhibited the proliferation of cancer cells in vivo and in vitro.

Several genes related to copper death, such as FDX1, LIAS, DLAT, and DLST, are abnormally expressed in cancer patients and are related to the occurrence, development, metastasis, and prognosis of the tumor. Targeted promotion of cell copper death and inhibition of cancer cell proliferation provide a new direction for future basic research in the field of tumor, which has public health significance for tumor prevention and treatment.

To study the drug resistance phenotype, drug resistance gene, and virulence gene of Helicobacter pylori(Hp) in patients with failed eradication therapy, and to provide reference for remedial therapy.

Hp was isolated and cultured from gastric mucosa of patients with failed eradication therapy. Drug sensitivity test was used to detect the resistance of isolated strains to Metronidazole (MTZ), Clarithromycin (CLA), Levofloxacin (LEV), Amoxicillin (AMX), Tetracycline (TET),and Furazolidone (FZ). CLA and LEV resistance genes 23S rRNA, gyrA and virulence genes cagA, vacA, iceA, oipA were amplified by PCR, to further explore the relationship between drug resistance phenotype and drug resistance and virulence genes.

In total 40 strains of Hp were isolated from 58 patients (69.0%). The resistance rates to MTZ, CLA, LEV, AMX, TET, and FZ were 100.0%, 82.5%, 72.5%, 0%, 0%, and 0%, respectively. The most common mutations of 23S rRNA and gyrA genes were T2182C (78.6%) and N87K (32.0%), respectively, and new C2165T and A2219G mutations were found in CLA resistant strains. In addition, the positive rates of virulence genes cagA, vacA s1, vacA s2, vacA m1, vacA m2, iceA1, iceA2, and oipA were 97.1%, 100.0%, 0%, 42.9%, 57.1%, 62.9%, 11.4%, and 68.6%, respectively, and the difference was statistically significant.

The resistance of Hp isolates to MTZ, CLA and LEV in patients with failed eradication therapy in this area is serious, and AMX, TET and FZ are recommended for remedial therapy. The relationship between CLA resistance and C2165T and A2219G mutation and LEV resistance and vacA m2 warrants further investigation.

To provide reference for improving and promoting the management and reform of practical ability training of Master of Public Health (MPH).

Based on the connotation of practical ability, combined with the characteristics of practical ability training in sample colleges, the USEM model of MPH practical ability was constructed, and the multiple realization paths that could improve MPH practical ability were extracted by fuzzy set qualitative comparative analysis.

Eight combined paths were identified including practical interest A* achievement motive B* no practical pressure ~ C* adaptability and strain capacy D* interpersonal communication E* team work and autonomous learning ability F* professional knowledge G* professional skill H* lack of analytical ability and judgement ~ I* implementation ability K* monitoring and evaluation ability L (* represented “and” while ~ represented “not”). Through the interactive comparison of different combination paths, it is found that practical interest, achievement motivation and self-monitoring and evaluation ability are the necessary conditions to improve the practical ability of MPH. Social adaptability had an important influence on the improvement of MPH practical ability. Practical pressure and implementation ability could complement each other in improving MPH practical ability. Practical interest, achievement motivation, analysis and judgment ability and decision planning ability were the core conditions to improve MPH practical ability.

It is suggested to improve practical interest, stimulate achievement motivation, strengthen self-monitoring and evaluation ability, pay attention to the cultivation of social adaptability and improve students’ comprehensive quality, restrict industry mentors by system to give students appropriate practical pressure, and explore the opportunity of situational education and strengthen the ability of situational practice.