To evaluate the dose-response relationship between obesity indicators and cholelithiasis in minority populations in Qiannan and Qiandongnan regions of Guizhou Province.

Based on the cross-sectional survey data of the "China Multi-ethnic Cohort Study", 17 084 adults aged 30-79 from the Buyi, Dong and Miao ethnic groups in Qiannan and Qiandongnan regions of Guizhou Province were included. The association between obesity indicators and cholelithiasis was analyzed using logistic regression models, and their dose-response relationship with cholelithiasis was studied using restricted cubic spline models.

The overall prevalence of cholelithiasis in Qiannan and Qiandongnan regions was 9.0%, with women at 9.6% higher than men at 7.5%. There were statistically significant differences between the cholelithiasis group and the non-cholelithiasis group in terms of gender, age, ethnicity, smoking history, hypertension, hyperlipidemia, BMI, LAP, VAI, WHtR, WHR, WC, weight, TG, HDL-C and GLU (P<0.001). Compared to the non-cholelithiasis group, the cholelithiasis group had higher age, weight, TG, GLU, BMI, VAI, LAP, WHtR, WHR, WC. In the logistic regression model, after adjusting for confounding factors, BMI, WC, WHtR, LAP, WHR were all statistically significant. As the values of obesity indicators increased, the risk of cholelithiasis also increased. Further analysis of their dose-response relationship revealed a nonlinear increasing trend in the association strength with cholelithiasis as the levels of BMI (P Nonlinear=0.004 6), LAP (P Nonlinear=0.000 4), WC (P Nonlinear=0.042 2), WHR (P Nonlinear=0.021) increased. As the level of WHtR (P Nonlinear=0.210 7) increased, the association strength with cholelithiasis showed a linear increasing trend.

Among the ethnic minorities in the Qiannan and Qiandongnan regions of Guizhou, there is a linear and nonlinear dose-response relationship between obesity indicators and gallstone disease. Advocating for residents to strengthen obesity control and prevention is an important means to reduce the incidence of gallstone disease.

Toanalyze the prevalence and determinants of chronic obstructive pulmonary disease (COPD) among the Hani ethnic minority older adults in rural areas of Yunnan Province.

The multi-stage stratified random sampling method was employed to select 1 402 rural Hani ethnic minority older adults aged ≥60 years in Mojiang County, Yunnan, each participant received a questionnaire survey and physical examination. Structure Equation Modeling (SEM) was applied to assess the possible influencing factors of COPD status.

Among the Hani ethnic minority older adults investigated, the prevalence of COPD was 8.84%. Men had a significantly higher prevalence of COPD than women (12.04% and 5.90%, χ²=16.348,P<0.001). Prevalence of COPD increased with advancing age (χ²=5.625,P=0.018). Hani older adults with a family history of COPD, individuals with low body mass index, and smokers had a higher prevalence of COPD than their counterparts (χ²=13.791,χ²=36.651,χ²=14.963,P<0.001). The results of SEM analysis indicated that gender, age, low body mass index, smoking had an effect on prevalence of COPD with the total effects of -2.53, 0.66, 1.79, 1.20 and 0.30, respectively.

The prevalence of COPD among Hani older adults was comparatively low in rural Yunnan, and men, advanced age groups, those with a family history of COPD, those with a low body mass index, and smokers were the priority populations for COPD prevention and control.

To explore the bidirectional causal relationship between multiple sclerosis (MS) and inflammatory bowel disease (IBD).

The dataset was pooled by genome-wide association studies (GWAS), of which MS data contained 14 498 samples and IBD data contained 24 865 samples, including 6 968 cases of ulcerative colitis (UC) and 17 897 cases of Crohn’s disease (CD). Random effect inverse variance weighting (IVW), weighted median (WME), MR-Egger regression, and weighted model were used for MR Analysis.

MS was found to increase the risk of UC (IVW: OR=1.108; 95% CI: 1.012-1.213; P<0.05), but not associated with the risk of CD (IVW: OR=1.021; 95% CI: 0.926-1.127; P>0.05); UC also increased the risk of MS, and the two were causal (IVW: OR=1.096; 95% CI: 1.027-1.169; P<0.05), and CD was associated with an increased risk of MS (IVW: OR=1.133; 95% CI: 1.003-1.279; P<0.05). After the MR-Egger regression intercept item test, it was found that there was no horizontal pleiotropy (P>0.05). The heterogeneity test results were all <0.001, so we should pay more attention to the random effects IVW model.

MS is associated with an increased risk of UC, but the effect on CD is not significant. IBD increases the risk of developing MS.

To investigate the prevalence of chronic pain in the elderly in Nanshan Pastoral area of Xinjiang, explore the influence of chronic pain degree on cognitive function, and the mediating role of depression and frailty.

From June 2022 to February 2023, 1 045 elderly patients ≥60 in Nanshan Pastoral area of Xinjiang were selected by multi-stage random cluster sampling method. The General Information Questionnaire, Pain Numerical Rating Scale, Geriatric Depression Assessment Scale, Tilburg Frailty Scale and Montreal Cognitive Assessment Scale were used to conduct the questionnaire survey.

The prevalence rate of chronic pain in the elderly in Nanshan pastoral area of Xinjiang was 67.27% (703/1 045). The degree of chronic pain was positively correlated with depression and frailty (r=0.472, 0.512, P<0.01), and negatively correlated with cognitive function (r=-0.432, P<0.01). Depression and frailty had significant mediating effect between chronic pain degree and cognitive function. The intermediate effects were -0.134 (95% CI:-0.194--0.074) and -0.145 (95% CI: -0.193--0.101), respectively. The mediating effect of depression-debilitation chain was also significant, and the mediating effect size was -0.121 (95% CI:-0.165--0.083). The total indirect effect of depression and frailty on the degree of chronic pain and cognitive function was -0.400, accounting for 53.33% of the total effect.

The degree of chronic pain not only directly affects the cognitive function of the elderly in Nanshan pastoral area of Xinjiang, but also indirectly affects the cognitive function through the independent mediating effect of depression and frailty and the chain mediating effect of depression and frailty.

To analyze the trend of incidence and mortality of liver cancer due to HBV (hepatitis B virus, HBV) infection in China from 1990 to 2019, and to provide a scientific basis for the prevention and control of liver cancer.

Using the Global Burden of Disease database, the incidence and mortality of liver cancer due to HBV infection in China were analysed from 1990 to 2019, and a Joinpoint regression model was applied to analyse the trends in the incidence and mortality of liver cancer due to HBV infection. An age-period-cohort model was constructed to analyze the effects of three factors: age, period, and cohort on changes in morbidity and mortality.

The overall trend of the standardized incidence rate and the standardized mortality rate of liver cancer due to HBV infection for both men and women in China from 1990 to 2019 showed a decreasing trend. The average annual rates of change in the standardized incidence rate for men and women were -2.94% and -4.24%, respectively. And the average annual rates of change in the standardized mortality rate were -3.35% and -4.45%, respectively. The age-period-cohort model showed that the risk of morbidity and mortality for males and females tended to increase and then decrease with age, and the overall trend decreased with period and birth cohort, but there was a slight increase locally.

The incidence and mortality rates of liver cancer caused by HBV infection show a substantial downward trend from 1990 to 2019. In the future, the problem of HBV-induced liver cancer will remain serious, and it is recommended that hepatitis B (Hepatitis B) vaccination be expanded among high-risk groups, and that public education on health behaviors be strengthened and early screening for liver cancer be actively carried out.

To explore the association of different glycemic measures and the risk of cancer.

A prospective cohort study conducted in Pudong New Area was used to solve the question. Cox proportional hazard model and Restricted cubic spline (RCS) regression model were used to explore the associations between different glycemic measures and the risk of cancer.

A total of 411 new cancer patients were observed after median 7.78 years follow-up, accumulative person-year incidence rates were 7.12/1 000. After multi-variable adjustment, the HR (95% CI) of elevated FPG and 2hPG were 1.10 (1.03~1.18) and 1.11 (1.02~1.20), respectively. The association between FPG and cancer risk showed a linear dose-response relationship; the association between 2hPG and cancer risk showed a trend toward a U-shaped dose-response relationship.

FPG and 2hPG are important risk factors for cancer, attention should be paid in the prevention and intervention of cancer.

Quality of life and survival time are important contents of palliative care services for patients with advanced cancer. Therefore, investigating the measurement method of quality-adjusted life years for patients with advanced cancer could provide a basis for the evaluation of palliative care services for this population.

The relative importance of each index in the Quality-of-Life Scale of Cancer Patients was compared by expert consultation method and hierarchical analysis was used to obtain the weight of each index.

In the Quality-of-Life Scale of Cancer Patients, the weights of primary indicators from high to low included physical symptoms, mental psychology, and social relations. The weights of physical symptoms from high to low included pain, side effects of treatment, sleep, daily life, appetite, fatigue, and facial expression. The weights of mental psychology from high to low included their cognition of disease, attitude, and spirit to treatment. The weights of social relations included the care and understanding of family and the understanding and cooperation of colleagues.

The weights of each quality of life scale indicator are obtained by hierarchical analysis. Then the different quality-of-life survival time is converted into quality-adjusted life years, which can provide a basis for the influence of palliative care services on the quality-adjusted life years of patients with advanced cancer.

To explore the role and mechanism of ferroptosis in exacerbating airway inflammation in asthmatic mice exposed to traffic-related PM_2.5.

Fifty BALB/c mice were randomly divided into five groups: saline (NS), asthma (ovalbumin, OVA), and OVA combined with low, medium, and high exposure to traffic-derived PM_2.5 (1.8, 3.6, and 7.2 mg/kg·bw, respectively). The levels of IL-6 and TNF-α in lung tissue were measured using ELISA kits. The concentrations of reduced glutathione (GSH), malondialdehyde (MDA), and Fe²⁺ in lung homogenate supernatants were assessed by colorimetry. Protein expressions of prostaglandin-endoperoxide synthase 2 (PTGS2), acyl-CoA synthetase long chain family member 4 (ACSL4), glutathione peroxidase 4 (GPX4), tumor protein p53 (p53), phospho-p53 (p-p53), spermidine/spermine N1-acetyltransferase 1 (SAT1), and arachidonate 15-lipoxygenase (ALOX15) were detected using western blot. mRNA expressions of PTGS2, ACSL4, GPX4, p53, SAT1, and ALOX15 were examined by qRT-PCR. One-way ANOVA was used for multi-group data comparison, followed by Tukey’s HSD for further multiple comparisons.

PM_2.5 exposure significantly increased IL-6 and TNF-α levels in asthmatic mouse lung tissues, especially in the high PM_2.5 exposure group (F_IL-6=17.910, P<0.001; F_TNF-α=5.414, P<0.05). All PM_2.5 exposure groups showed a reduction in GSH, most notably in the high exposure group (F_GSH=13.560, P<0.001). MDA and Fe²⁺ levels were significantly higher in the medium and high PM2.5 groups compared to the OVA group (F_MDA=55.230, P<0.001; F_Fe=17.660, P<0.001). PM_2.5 exposure also elevated the protein and mRNA expressions of PTGS2, ACSL4, and ALOX15, while reducing GPX4 expression. The protein and mRNA expression levels of p53, SAT1, and ALOX15 showed a clear dose-response to PM_2.5, with p-p53 following a similar trend as p53 protein expression.

Traffic-related PM_2.5 may induce ferroptosis in the lungs of asthmatic mice and exacerbate asthma inflammation through the p53/SAT1/ALOX15 pathway.

To explore the association between the dietary diversity score (DDS) and the prevalence of possible sarcopenia among the elderly.

We conducted this analysis utilizing the cross-sectional data from the Chinese longitudinal healthy longevity survey and happy family study(CLHLS-HF, wave 2018 ). A standard developed by the Asian Working Group for Sarcopenia 2019(AWGS2019) was used to assess the possibility of sarcopenia. We have constructed four new indicators of DDS as follows: total diet, animal-based diet, plant-based diet, and plant-based diet without the consumption of legume products and nuts. We used the Logistic regression analysis to evaluate the association between the DDS of the total diet, animal-based diet, plant-based diet, and plant-based diet without the intake of legume products and nuts and possible sarcopenia. These associations were statistically adjusted for a variety of potential confounders. Sensitivity analysis and subgroup analyses were performed.

The analysis included 2 555 participants (mean age of 84.4 years). 1 244 (48.7％) were male and 1 311 (51.3％) were female. 1 632 elderly people had possible sarcopenia. DDS in the possible sarcopenia group was significantly lower than that in the non-sarcopenia group (P < 0.001). In our study, we found that participants with a higher DDS of the total diet (OR=0.544, 95% CI：0.405-0.732), animal-based diet (OR=0.613, 95% CI：0.423-0.888), and plant-based diet (OR=0.469, 95% CI：0.321-0.684) and plant-based diet without the consumption of legume products and nuts (OR=0.382, 95% CI：0.206-0.709) were at a lower risk of developing sarcopenia. In sensitivity analyses, the associations remained unchanged. In subgroup analysis, total diets, animal-based diet, and plant-based diet were still associated with a reduced risk of possible sarcopenia in the male population, while plant-based diet and plant-based diet without the intake of legume products and nuts were associated with a reduced risk of possible sarcopenia in the female population.

Taking a diversified diet may reduce the risk of developing sarcopenia. According to the findings of this study, adopting a diversified diet might reduce the risk of sarcopenia for older adults.

To investigate the causal association between estrogen and breast cancer incidence and mortality risk.

Using summary data from genome-wide association studies, with estradiol representing estrogen, genetic variants associated with estradiol were utilized as instrument variables.Employing the univariate Mendelian randomization method, the causal association between estradiol and the risk ofincidence (4 outcomes), as well as the risk of mortality (6 outcomes) of female breast cancer and its subtypes, were assessed by the inverse variance-weighted method.

The results revealed that elevated levels of estradiol (≥175 pmol/L)were associated with an increased risk of overall breast cancer (OR=1.43,95% CI:1.27-1.60,P=2.92×10^-9), estrogen receptor-negative breast cancer (OR=1.41, 95% CI:1.25-1.60, P=2.16×10^-8), and estrogen receptor-positive breast cancer (OR=1.49, 95% CI:1.29-1.72, P=6.92×10^-8) incidence. However, there was no evidence that elevated levels of estradiol were the risk factors for triple-negative breast cancer incidence. Moreover, overall breast cancer and its subtypes mortality risks were not shown to be causally related to estradiol.

Elevated estrogen levels (≥175 pmol/L) are associated with an increased risk of overall breast cancer, estrogen receptor-negative breast cancer, and estrogen receptor-positive breast cancer incidence.

To assess the prevalence and identify risk factors of thyroid diseases among perimenopausal women, providing a theoretical basis for scientific prevention and control measures.

533 perimenopausal women in Shenzhen, aged 40-60, were statistically analyzed for demographics, living habits, residential pollution, and biochemical indicators, with multionmial logistic regression used to assess their links to thyroid disease occurrence.

The prevalence of thyroid dysfunction in perimenopausal women was 37.0% (197/533). The prevalence of thyroid nodules was 49.7% (265/533) and 14.3% (76/533) in other thyroid ultrasound abnormalities types. The prevalence of thyroid ultrasound abnormalities accordingly tended to increase with age. Multionmial logistic regression analysis showed that systolic blood pressure (OR=1.066, 95% CI:1.024-1.110) and history of thyroid disease (OR=6.853, 95% CI:1.537-30.555) were the risk factors of thyroid dysfunction; 55-60 years old (OR=5.011, 95% CI:1.938-12.956), occupation of clerical staff (OR=3.332, 95% CI:1.196-9.282), diastolic blood pressure (OR=1.050, 95% CI:1.004-1.098), and history of thyroid disease (OR=6.704, 95% CI:1.820-24.698) were the risk factors of thyroid nodules; TGAb antibody positivity (OR=3.763, 95% CI: 1.177-12.031), occupation of technician (OR=4.196, 95% CI:1.259-13.983), systolic blood pressure (OR=1.036, 95% CI:1.005-1.068) and history of thyroid disease (OR=17.917, 95% CI:5.107-62.851) were the risk factors of thyroid dysfunction and ultrasound.

Thyroid dysfunction and nodule rates among perimenopausal women in Shenzhen (37.0% and 49.7%) exceed China's averages (19.9% and 23.7%). Heightened screening and early action for thyroid issues in this group are needed.

To explore the longitudinal trajectory of systolic blood pressure (SBP) and its influencing factors in community hypertensive patients.

We used data from national basic public health services for nine counties in Shandong Province to conduct a prospective cohort study. Group-Based Trajectory Modeling (GBTM) was used to explore the trajectory of systolic blood pressure. Repeated measurement analysis of variance was used to describe the changes in trajectories, and multinomial logistic regression was used to explore the influencing factors of the trajectories.

A total of 104 402 hypertension patients were included. The systolic blood pressure trajectories could be divided into four groups: Stage-1 high blood pressure gradually decreased group (21.8%), Stage-1 high blood pressure stable group (55.5%), Stage-2 high blood pressure gradually decreased group (7.2%) and Stage-2 high blood pressure increased group (15.6%). The results of repeated measures analysis of variance showed that there was a significant difference in systolic blood pressure values at different time points or different groups (P<0.001, P<0.001). The results of multinomial logistic regression showed that compared with the group with Stage-1 high blood pressure gradually decreased, the people with less education(OR=82.68, 95% CI:75.60-90.42), physical inactivity(OR=2.77, 95% CI:2.19-3.50), high frequency of drinking(OR=1.39, 95% CI:1.17-1.64), general compliance behavior(OR=1.44, 95% CI:1.18-1.75), intermittent medication(OR=2.15, 95% CI:1.98-2.34) were more likely to be classified into the group with Stage-2 high blood pressure increased. People with increased age (OR=1.03, 95% CI:1.02-1.03), and regular medication (OR=1.94, 95% CI:1.79-2.11) were more likely to be classified into the group with Stage-2 high blood pressure gradually decreased.

The systolic blood pressure trajectories in hypertensive patients can be divided into 4 categories. In the future, we should make more efficient assessments and interventions based on homogeneous populations of systolic blood pressure trajectories and their risk factors.

To explore the impact of health checkups on the utilization of inpatient services for patients with chronic diseases to promote the utilization of health checkups and alleviate the burden of hospitalization.

Utilizing data from four waves of the China Health and Retirement Longitudinal Study conducted in 2011, 2013, 2015, and 2018, this study employed coarsened exact matching (CEM) and the difference-in-difference（DID）and BK method to analyze the impact of health checkups on the utilization of inpatient services for patients with chronic diseases, along with examining its influencing mechanisms.

Health checkups were found to increase the probability of hospitalization by 34.4% (β=0.344, 95% CI: 0.132-0.556) and reduce hospitalization costs by 3 145 yuan (β=-3 145.470, 95% CI: -6 074.532--216.407) for patients with chronic diseases. Exercise was identified as mediating the association between health checkups and hospitalization (β=-0.184, 95% CI: -0.323--0.045). Age was observed to negatively moderate the relationship between health checkups and hospitalization (β=-0.531, 95% CI: -0.804--0.258). Patients with multiple chronic diseases and groups with health insurance were more significantly affected by health checkups.

Health checkups increase the likelihood of hospitalization and reduce hospitalization costs for individuals with chronic diseases. Promoting healthy lifestyles among those with chronic conditions is essential, with a specific focus on groups susceptible to health risk shocks.

To comprehensively evaluate the vulnerability level of elderly farmers and herdsmen’s family old-age pension and analyze the obstacle factors.

Based on the vulnerability framework, we constructed a family old-age pension vulnerability assessment index system, and selected 2 594 research subjects for questionnaire survey by multi-stage random cluster sampling. We applied entropy-weight-TOPSIS method to comprehensively evaluate the level of elderly farmers and herdsmen’s family old-age pension vulnerability, and explored the obstacle factors.

60.14% of the elderly farmers and herders in the southern mountainous pastoral areas of Xinjiang had medium or high levels of vulnerability to old age; the three dimensions of vulnerability to old age and their degree of obstacles were sensitivity (47.59%), adaptive capacity (27.73%), and exposure (25.58%); the top five obstacles and their degree of obstacles were hospitalization due to illness (11.86%), physical fitness index (10.86%), and physical health index (11.86%); and the top five obstacles and their degree of obstacles were hospitalization due to illness (11.86%). The top five barriers and their degree of obstacles were hospitalization due to illness (11.86%), body mass index (10.17%), debt (10.06%), caring for grandchildren (9.16%), and self-care ability (5.70%); and the degree of obstacles to care for the elderly was the fourth highest among elderly farmers (8.62%) and the fourteenth highest among elderly pastoralists (2.69%).

There are differences in vulnerability levels and obstacles between elderly farmers and herders. It is recommended to pay attention to the differences in the characteristics of elderly farmers and herders themselves and their pension resources, so as to formulate more accurate pension strategies.

The purpose of this study was to investigate loneliness and its influencing factors among female migrant elderly following children (MEFC) in Jinan City, based on which to recommend suggestions to alleviate the loneliness of female MEFC.

The data came from a survey conducted in 2020 in Jinan City, on the MEFC, and 418 valid samples were obtained by using multistage cluster sampling. The ULS-8 scale was used to measure the loneliness of female MEFC, and the Social Support Rating Scale (SSRS) was used to measure the social support status. t-test and one-way ANOVA were used to test the differences in loneliness of female MEFC with different characteristics, and multiple linear regression models were used to investigate the influencing factors of the loneliness of female MEFC.

371 (88.8%) female MEFC came from rural areas, 369 (88.3%) of them moved to take care of their grandchildren, the social support score of female MEFC was (39.5±6.0), the loneliness score was (12.9±4.0), and the factors influencing loneliness included: personal monthly income(β=-0.17, P<0.001,95% CI：-3.337--1.007), type of household registration (β=-0.12, P=0.008, 95% CI:-2.523--0.385), year of migration (β=-0.09, P=0.042, 95% CI: -2.155--0.038), perception of being a local (β=-0.17, P=0.018, 95% CI: 0.267-2.840), the frequency of participation in community square dance (β=-0.18, P<0.001, 95% CI: -3.614--1.092) and community-organized activities (β=-0.13, P=0.013, 95% CI: -2.636--0.306), and social support (β=-0.19, P<0.001, 95% CI: -0.186- -0.064).

The level of loneliness among female MEFC was lower than that of female empty nesters but higher than that of male elderly. To alleviate the loneliness of female MEFC, joint efforts are needed from the communities where female MEFC live, family members of female MEFC, and female MEFC themselves.

To explore the relationship between Internet use and cognitive function in the elderly.

Based on the data of China Family Panel Survey (CFPS) in 2020, the IADL scale and the mobile center self-rating depression scale were used to measure the disability status and depression status of the elderly. Two-column correlation analysis was used to analyze the correlation. This paper constructed a multiple mediating effect model to study the mediating path of the impact of Internet use on the cognitive function of the elderly.

After controlling for age, gender, marital status, and intergenerational support, the regression results showed that the use of the network had a significant direct impact on the cognitive function of the elderly (β=0.688, t=7.36, P<0.001), and depression and disability significantly affected the cognitive function of the elderly (β=-0.018, t=-2.696, P<0.05; β= -0.114, t=-4.83, P<0.001). The results of mediating effect analysis showed that the simple mediating effects of depression and disability between Internet use and cognitive function of the elderly were 0.044 (95% CI: 0.011 3-0.083 4) and 0.040 (95% CI:0.015 8-0.064 2), respectively, and the chain mediating effect was 0.006 (95% CI:0.000 4-0.013 2).

Depression and disability status have a mediating effect between whether or not to use the network and the cognitive function of the elderly, and depression and disability have a chain mediating effect in whether or not to use the network to affect the cognitive function of the elderly. With the popularization of the Internet, we should promote the use of the Internet in the elderly, and pay attention to the physical and mental health of the elderly, so as to protect the cognitive function of the elderly and delay or prevent the occurrence of cognitive impairment in the elderly.

To investigate the sedentary behavior of middle-aged and elderly non-diabetic population in Lanzhou and explore its correlation with insulin resistance (IR).

A cluster random sampling method was used to select residents aged 40-75 years from the survey data of the "REACTION Study" conducted between 2014 and 2016 in Lanzhou. A total of 3 609 individuals who met the inclusion criteria were included in this study. The sedentary time was divided into <3 h/d, 3-5 h/d and ≥5 h/d groups. Insulin resistance was diagnosed using the homeostasis model assessment of insulin resistance (HOMA-IR). Multivariate logistic regression analysis was performed to examine the association between sedentary behavior and IR, while subgroup analysis explored this relationship among different population subgroups.

The average daily sedentary time for study population was found to be approximately 4.00±1.92 h/d, with an overall prevalence rate of IR at 25.0%. The prevalence rates of IR for those with a sedentary time <3 h/d, 3-5 h/d and ≥5 h/d were observed as follows: 21.7%, 24.9% and 31%, respectively, with statistically significant differences (χ²=24.447, P<0.05). After adjusting for various confounding factors through logistic regression analysis, it was revealed that compared with sedentary <3 h/d, sedentary ≥5 h/d was a risk factor for the prevalence of IR (OR=1.468, 95% CI:1.168-1.845, P<0.05). Subgroup analysis indicated that only within subgroups consisting of individuals aged≥65 years old (OR=1.060,95% CI:1.005-1.118), insufficient physical activity (OR=1.071, 95% CI:1.017-1.128), hyperlipidemia (OR=1.071,95% CI:1.001-1.145), no hyperuricemia (OR=1.062, 95% CI:1.010-1.117), fatty liver (OR=1.133, 95% CI:1.038-1.237), and WC≥85 cm (OR=1.079, 95% CI:1.017-1.144), increased sedentary time was associated with increased risk of IR (all P<0.05).

Sedentary behavior is positively associated with the occurrence of IR in middle-aged and elderly non-diabetic population.However, the findings may vary across populations with distinct characteristics.

To identify the types of social networks of the elderly in China, to analyze their population distribution characteristics, and to explore the association between different social network types and health behaviors, so as to provide reference for developing personalized health intervention strategies.

Using the data from the 2018 Chinese Longitudinal Healthy Longevity Survey, latent class analysis was used to identify the types of social networks in the elderly. The Multinomial Logistic Regression Analysis and Apriori algorithm were used to analyze the association between different social network types and health-related behaviors.

A total of 6 184 elderly people was included in this study. The social network types of the elderly in China were divided into family type (45.97%), couple type (41.91%) and widowed type (12.12%).Results from the Multinomial Logistic Regression Analysis showed that the elderly who were older (OR=1.141, 95% CI: 1.132-1.149), female (OR=4.472, 95% CI: 3.846-5.201), in ethnic minority (OR=1.454, 95% CI:1.037-2.040), and had sufficient sleep (OR=1.158,95% CI: 1.010-1.328) were more likely of the family-oriented social network. The elderly who were older (OR=1.120, 95% CI: 1.109-1.131), female (OR=4.913, 95% CI: 4.007-6.026) and had sufficient sleep (OR=1.247,95% CI:1.039-1.498) were more likely of the widowed-like social network.Strong association rules of the association rule is: drinking, smoking, and exercise of the elderly social network for couples.

The couple type is more likely to have unhealthy behaviors in the elderly in China, and its social network is smaller and its social support is single. Therefore, it is necessary to enrich the social network, enhance social support, and carry out intergenerational intervention to improve the healthy behavior of the elderly.

To investigate the effect and mechanism of HBV-stimulated HTR8-S/Vneo mitochondrial antiviral signaling protein (MAVS) signaling pathway on the immune response of peripheral blood mononuclear cell (PBMC) to hepatitis B vaccine.

The HTR8-S/Vneo were co-incubated with serum of HBV-DNA positive patients. MAVS siRNA transfection was hereby applied for silencing of MAVS and MAVS plasmid transfection was applied for overexpression of MAVS in HTR8-S/Vneo. We then cocultured them with human immune cells assembly PBMC and subsequently stimulated with hepatitis B vaccine. The mRNA and protein expression levels of MAVS, NF-κB, pNF-κB, IRF3 and pIRF3 in HTR8-S/Vneo were detected by reverse transcription-quantitative real-time PCR (RT-qPCR) and flow cytometry (FCM). The levels of IL-2, IL-6, IFN-β and IL-17 were detected by enzyme-linked immunosorbent assay (ELISA), and the proportion of CD4⁺T cell were detected by flow cytometry. The data were analyzed for t test.

HBV down-regulated the mRNA expression levels of MAVS, NF-κB, IRF3 (t=6.66, P=0.003; t=14.18, P<0.001; t=3.70, P=0.021) and the protein expression levels of MAVS, NF-κB, pNF-κB (t=3.42, P=0.042; t=4.23, P=0.013; t=4.86, P=0.008), and inhibited the production of IFN-β (t=9.83, P=0.010). Silencing MAVS down-regulated the mRNA and protein expression of MAVS (t=31.20, P<0.001; t=6.53, P=0.023), inhibited the expression of IFN-β (t=5.46, P=0.032), and reduced the proportion of CD4⁺T cells in PBMC (t=9.07, P=0.001). Overexpression of MAVS induced IFN-β production (t=-34.26, P<0.001; t=-5.11, P=0.036), but the proportion of CD4⁺T cells did not show significant effect (t=-0.73, P=0.506).

HBV can inhibit the MAVS signaling pathway in HTR8-S/Vneo. Overexpression of MAVS could promote the immune response to hepatitis B vaccine in PBMC.

To explore the relationship between retinol and TTR protein, and to provide ideas for preventing Hereditary vitreous amyloidosis (HVA).

Twenty Gly83Arg mutant mice were constructed and bred (reference construction ^[2]) as the mutant group. After the irregular turbidity in the vitreous of the mutant mice eyeball was observed under slit lamp, the lens camera was used for photography, and B-ultrasound examination was conducted, and white clump-like turbidity was found in the posterior ocular region. After the turbidity in the vitreous cavity of the mice was confirmed, the mice were killed by cervical vertebrae dislocation, and DNA in liver cells was extracted and sequenced to confirm the successful construction of mutant mice. At the same time, 20 wild type C57BL/6 mice were fed as the control group, 4 mice in the mutant group and 4 mice in the control group were randomly selected from the number table, and the retinol concentration was determined by high performance liquid chromatography combined with mass spectrometry. Three mice in the mutant group and three mice in the control group were selected from a random number table. After grinding the eyeball tissues, RNA and protein were extracted. TTR gene mRNA expression, SDS-PAGE protein gel color development was detected by fluorescent quantitative PCR, and TTR gene protein expression was detected by Western Blot. The difference at P < 0.05 was statistically significant.

The mutation site was consistent with the site successfully modeled by our research group in the previous stage. Heterozygous mutation occurred at the G base at the 107th position of exon 3 (C.107G →C), and the codon of the amino acid at the 83rd position was mutated from GGC to CGC (Gly83Arg). The concentration of retinol in eye of mutant mice was lower than that of normal mice in control group, the content of retinol in liver tissue of mutant mice was higher than that in control group, and the content of retinol in blood of mutant mice was lower than that in control group (eyeball: t=6.600, P=0.022; liver: t=9.045, P<0.001; blood: t=4.343, P=0.012). Fluorescence quantitative PCR results showed that mRNA expression in eyeball tissue of mutant group was lower than that of control group (t=5.764，P=0.004). SDS-PAGE protein gel color showed that protein bands appeared in the mutant group near 11kD. Western Blot showed that TTR gene protein expression in mutant group was lower than that in control group (t=37.48，P<0.001).

The deposition of vitreous amyloid protein in the eyeball tissue of Gly83Arg mutant mice may be related to the increase of mutant TTR protein and the decrease of retinol, which has important significance for future prevention and treatment.

To explore the longitudinal bidirectional relationship between depressive symptoms and HIV infection risk behaviors among young men who have sex with men (YMSM).

A longitudinal study design was used to recruit 489 YMSM from September 2017 to January 2018 (T1), and two follow-up surveys were completed from July to September 2018 (T2) and from June to July 2019 (T3), respectively. The cross-lagged path analysis model was used to analyze the causality, time series relationship and interactive direction between depressive symptoms and HIV infection risk behaviors.

Data of 347 YMSM were analyzed using cross-lagged path analysis. The results showed that T1 depressive symptoms significantly and positively predicted T2 HIV infection risk behaviors (β=0.071, 95% CI: 0.000-0.142), and T2 depressive symptoms significantly and positively predicted T3 HIV infection risk behaviors (β=0.072, 95% CI: 0.000-0.143). However, T1 HIV infection risk behaviors did not significantly predict T2 depressive symptoms and T2 HIV infection risk behaviors did not significantly predict T3 depressive symptoms.

Depressive symptoms among YMSM significantly and positively predicted HIV infection risk behaviors, with a unidirectional causal temporal relationship. Therefore, the level of depressive symptoms should be considered as an essential factor in HIV infection risk behavior interventions for YMSM.

To investigate the relationship between 24-hour urinary sodium, urinary potassium, sodium-potassium ratio and microalbuminuria among people in elderly care institutions.

Questionnaire survey, physical examination, blood and 24 h urine samples collection were carried out in the elderly people of 16 elderly care institutions in southeastern Shanxi from September 2017 to November 2017, which were selected using the random cluster sampling method. A multivariate logistic regression model was used to analyze the association between 24 h urinary sodium excretion, urinary potassium excretion, sodium-potassium ratio and MAU.

A total of 502 subjects including 467 males (93.03%) and 33 MAU (6.57%) were included in this study (average age: 65.77±8.54 years old). The 24 h urinary sodium excretion was (193.02±87.64) mmol, urinary potassium excretion was (27.95±15.02) mmol, and the sodium-potassium ratio was (7.44±2.62), respectively. Multivariate logistic regression analysis showed that group with the highest urinary sodium excretion and the sodium-potassium ratio (Q4) compared to the lowest group (Q1) was associated with an increased risk of MAU (OR=4.026, 95% CI: 1.045-15.515; OR=4.043, 95% CI: 1.252-13.057).

The 24-hour urinary sodium and sodium-potassium ratio are positively associated with the prevalence of MAU, while 24-hour urinary potassium is not related to the prevalence of MAU.

To investigate the epidemiological characteristics and treatment outcomes of elderly patients with rifampicin-resistant tuberculosis (RR-TB) in Chongqing, and to provide evidence for the formulation of prevention and control policies for drug-resistant tuberculosis in elderly population in Chongqing. MethodsData of RR-TB patients registered in Chongqing from 2011 to 2022 were collected through the tuberculosis surveillance module of National Health Security Information Project-Disease Prevention and Control Information System. Then, RR-TB patients over 65 years were screened. Retrospectively, descriptive epidemiological methods were used to analyze the epidemiological characteristics and treatment outcomes of elderly patients with RR-TB, and to compare them with RR-TB in the whole population.

A total of 5 797 RR-TB patients were registered in Chongqing from 2011 to 2022, among which 885 were over 65 years old, accounting for 15.27%. The annual average patient registration rate of elderly RR-TB patients was 1.81/100 000, showed an increasing trend year by year.(,P<0.05). The elderly RR-TB patients were mainly male (80.90%), local population(75.03%), farmers (56.61%) and re-treatment (57.18%). The elderly RR-TB patients of local population, farmers and newly diagnosed patients increased year by year (,22.904,21.581, all P<0.05). In the number of elderly RR-TB patients, the central urban area accounted for the most (29.49%), and the city cluster of Wuling Mountain area in southeast Chongqing. There were statistical differences between different areas. In terms of region, the new area of the main city accounted for the most (29.49%), and the town of Wuling Mountain area in southeast Chongqing accounted for the lowest (12.88%), There were statistical differences between different areas (χ²=42.422, P<0.05). The treatment acceptance rate of elderly RR-TB patients registered from 2011 to 2020 was 83.52%, and the treatment success rate was only 34.59%. The main reasons for unsuccessful treatment were death (19.96%)and loss to follow(28.60%).

The epidemic situation of RR-TB in the elderly is serious.Initial drug resistance, treatment acceptance, standardized management should be paid special attention.

To intervene in the process of the information epidemic's impact on public anxiety through a mediated model with moderation, and to explore the relationship between information epidemic and anxiety and its mechanism of action, with a view to better solving the difficulties it poses, eliminating the atmosphere of public anxiety shrouded by the information epidemic under the public health emergencies, and further stifling the possibilities of the generation of social risk crises.

Using a combination of convenience sampling and snowball sampling, 1 580 valid questionnaires were surveyed nationwide, and descriptive statistical analyses, regression analyses, and mediated moderated effects analyses were conducted.

Information epidemic had a positive predictive effect on anxiety level (β=0.14, P<0.001), mental toughness partially mediated the relationship between information epidemic and anxiety level 0.30 (95% CI:0.10-0.52), with the indirect effect accounting for 15.4 % of the total effect; and education level played a moderating role in the second half of the pathway of the aforementioned mediation model (β=-0.36, P<0.001).

In the context of public health emergencies, it is recommended to improve the public's mental toughness and education level to mitigate the adverse impacts of information outbreaks, and then reduce the negative emotions of public anxiety.

To discuss the risk factors of imported food through the multi-dimensional comparative analysis of the food information published by the General Administration of Customs and to provide data reference for supervisors, and to improve the supervision of imported food.

This paper mainly collected and summarized the 48 periods of non-permitted entry food data released by the Import and Export Food Safety Bureau of the General Administration of Customs during 2019-2022 and made a comparative analysis of the sources of non-permitted entry food, food production enterprises, product types, non-permitted entry facts and other dimensions.

From 2019-2022, there were a total of 9 506 batches of food that were not allowed to enter the country, and the food that was not allowed to enter the country came from 112 countries and regions, with the highest proportion of food batches from Japan. The food came from 3 941 production enterprises, where11 countries or regions holding non-permitted entry food production enterprises, accounting for more than 50% of the total number of enterprises. According to the HS code, there were six categories and 21 chapters of non-permitted entry products, and the main non-permitted entry foods were HS03, 21, 19 and 22 products, more than 60% of the total unqualified. According to the analysis of the causes of nonconformity, there were 15 kinds, and the top 6 nonconformity causes accounted for 86% of the total nonconformity batches. There was a total of 268 types of unqualified items related to quality and safety, totaling 3 469 items.

The overall number of batches of unauthorized imported food during the period of 2019-2022 is a rising and then falling trend, and the unauthorized imported food is more concentrated in the countries of origin, manufacturers, categories of unauthorized food, reasons for failure, and quality-related unqualified items. The multi-dimensional comparative analysis provides data reference for regulators and consumers and helps the government to improve the regulation of imported food.

To analyze the unqualified situation of blood test of voluntary blood donors in Changsha from 2018 to 2022, and to provide reference for the formulation of recruitment strategy of voluntary blood donors.

From January 2018 to December 2022, 792 508 voluntary blood donors in Changsha area (Changsha Blood Center) were selected as the subjects of this study. Blood samples were tested by enzyme-linked immunosorbent assay (ELISA) twice and nucleic acid detection (NAT) once. The unqualified blood tests in different years and populations were described.

The total unqualified rate of voluntary blood donors in Changsha from 2018 to 2022 was 1.76%, with the highest rate in 2018 (1.90%) and the lowest rate in 2022 (1.56%). The total failure rate of alanine aminotransferase (ALT) and hepatitis B virus surface antigen (HBsAg) ranked first (1.00%) and second (0.62%), respectively. There were significant differences in the unqualified rate of blood test for HBsAg, anti-HCV, anti-HIV, anti-TP, ALT and the single reactivity rate of nucleic acid test (NAT) among different years (P<0.05), and there were significant differences in the unqualified rate of blood test for volunteer blood donors with different gender, age, education level and occupation (P<0.05).

The total unqualified rate of blood testing of voluntary blood donors in Changsha from 2018 to 2022 has significantly decreased compared with that before 2018 in the same region, and ALT and HBsAg are the main reasons for unqualified blood testing. In order to promote the sustainable and healthy development of voluntary blood donation in Changsha, it is necessary to further improve the blood testing technology, optimize the blood collection and supply process, and then improve the accuracy of the test results.

To investigate the relationship between serum cystatin C (CysC) levels and the risk of frailty.

This study used data from the 2011 China Health and Retirement Longitudinal Study (CHARLS). Through questionnaires and physical examination, 32 items of information were collected from the respondents, such as illness, ability to perform daily activities to calculate the frailty index (FI) to assess the state of frailty. A multivariate logistic regression model was used to explore the relationship between CysC levels and frailty, while restricted cubic spline was used to analyse the non-linear relationship.

A total of 6 941 adults aged 45 years and older, with a mean age of 59.2±9.8 years, 48.1% of whom were male, were included in the study, with a total of 1 004 (14.5%) experiencing frailty. After adjusting for confounders in the multivariate logistic regression model, the risk of developing frailty in the group with the highest CysC level (T3) was 2.13 times higher than that in the group with a lower CysC (T1) (OR =2.13, 95% CI: 1.75-2.60). When CysC was used as a continuous variable, the risk of frailty increased 1.89-fold for each unit increase in CysC. Restricted cubic spline function analyses showed an approximately "J" shaped relationship between CysC levels and the risk of debility (P _non-linear<0.05), the risk of frailty increased significantly with increasing CysC levels when CysC levels were ≥1.05 mg/L.

CysC levels are closely related to frailty, and there is a nonlinear relationship between them.

To analyze the treatment outcomes and influencing factors among pulmonary tuberculosis patients in Guizhou Province from 2017 to 2022, and to improve a basis for enhancing tuberculosis prevention and control strategies.

Individual cases of pulmonary tuberculosis patients in Guizhou Province from 2017 to 2022 were obtained from The Tuberculosis Information Management System. The distribution characteristics of treatment success were described using percentages (%), and the Chi-square test and binary logistic regression model were utilized to analyze the factors influencing treatment outcomes.

During the period of 2017 to 2022, the annual average registration rate of pulmonary tuberculosis patients in Guizhou Province was 97.27/100 000 population, exhibiting a consistent downward trend (trend χ²=2 230.684, P<0.001). Meanwhile, the treatment success rate among the patients during the same time period was 92.89%, demonstrating a steady increase (trend χ²=51.800, P<0.001). Analysis results of influencing factors showed that compared with female, age group <20 years old, cadres and staff, physical examination, negative etiological results, initial treatment, no comorbidities, no other tuberculosis, and negative HIV test results, male (OR=0.736, 95% CI:0.709-0.765), ≥60 years old age group (OR=0.360, 95% CI:0.323-0.402), farmers (OR=0.591, 95% CI:0.468-0.747), medical treatment due to illness (OR=0.716, 95% CI: 0.599-0.855), retreatment (OR=0.570, 95% CI:0.540-0.602), complications (OR=0.789, 95% CI:0.752-0.827), other tuberculosis (OR=0.685, 95% CI:0.542-0.867) and positive HIV test results (OR=0.387, 95% CI:0.342-0.437) were risk factors for successful treatment(P<0.05).

Overall, the treatment outcomes of pulmonary tuberculosis patients in Guizhou Province are highly positive. However, it is essential to recognize that there are disparities in the distribution of treatment outcomes across various population groups. Hence, it is recommended to prioritize the treatment concerns of specific demographics, including male patients, the elderly population, individuals residing in Bijie city, patients with positive microbiological results, HIV=positive patients, individuals requiring retreatment, and those with comorbidities or concurrent tuberculosis. By focusing on these groups, targeted interventions and tailored approaches can be implemented to ensure that all individuals receive optimal treatment and care.

To explore the causal relationship between type 2 diabetes mellitus (T2DM) and the risk of lung cancer (LC) (including three subtypes: lung adenocarcinoma (LAC), lung squamous cell carcinoma (LSCC), and small cell lung carcinoma (SCLC)) using a two-sample Mendelian randomization approach (MR).

The analysis was performed using summary datasets of genome-wide association studies (GWAS) conducted in European ancestry. Using 380 genome-wide significant single nucleotide polymorphic loci (SNP) associated with type 2 diabetes mellitus as instrumental variables (IV), the primary analysis used inverse variance weighted (IVW) method, MR-Egger regression and weighted median estimator method to investigate the causal association between type 2 diabetes mellitus and lung cancer and its subtypes. Sensitivity analyses were performed including MR-Egger regression intercept test, removing SNPs associated with potential confounding traits, excluding palindromic SNPs, and “leave one out” method.

IVW method showed that genetically predicted type 2 diabetes mellitus was not associated with the risk of lung cancer and its subtypes (LC OR: 1.003, 95% CI: 0.974-1.034; LAC OR: 0.981, 95% CI: 0.943-1.021; LSCC OR: 1.043, 95% CI: 0.995-1.093; SCLC OR: 0.988, 95% CI: 0.921-1.061, respectively). The results remained consistent by using MR-Egger method and weighted median estimator method. The robustness of results was collaborated by several sensitivity analyses. Conclusions This study dosen’t provide evidence supporting the causal relationship between type 2 diabetes mellitus and the risk of overall lung cancer and its subtypes.

To develop a sarcopenia cognitive behavior questionnaire for elderly in community and to test the reliability and validity of the questionnaire.

Based on cognitive behavior theory, the initial questionnaire was formed through literature review and expert consultation. We used convenience sampling method selected a total of 240 elderly people from 8 communities, including Fuhua Street community, Nanyang Road community, Mianfang Road community and Zijingshan Road community as survey objects from October to December 2022. Then, we modified the questionnaire and tested for reliability and validity.

The sarcopenia cognitive behavior questionnaire of community elderly included 3 dimensions and 23 items. The questionnaire’s total Cronbach’s coefficient was 0.903, and the split-half reliability was 0.872, and the test-retest reliability coefficient was 0.901 two weeks later. I-CVI of questionnaire was 0.857-1.000 and S-CVI/Ave was 0.988. Six common factors were extracted from exploratory factor analysis, and the cumulative variance contribution rate was 69.745%.

The sarcopenia cognitive behavior questionnaire of community elderly is scientific and practical, and can be used as a tool for clinical workers to evaluate the sarcopenia cognitive status of community elderly and their prevention and management behavior.

To construct an evaluation index system of information literacy for public health personnel, and to provide reference for assessing the level of information literacy of public health personnel.

The indicator pool of the indicator system was constructed through the literature research method and combining with the actual work, 19 experts in related fields were invited to conduct two rounds of expert consultation by the Delphi method, so as to determine the final indicators, and the weights of indicators at all levels were determined by the hierarchical analysis method.

The positive coefficients of the two rounds of expert consultation were greater than 90%, and the expert authority coefficients were 0.895 and 0.922. The expert Kendall coordination coefficients were 0.152 and 0.121, respectively P<0.001, and the differences were all statistically significant. Through two rounds of expert consultation, the evaluation index of information literacy of public health personnel constructed by 4 first-level indicators, 15 second-level indicator systems and 38 third-level indicators of information awareness, information knowledge, information competence and information utilization ethics were finalized.

The index system has certain reliability and scientificity, and can provide a reference basis for measuring the information literacy level of public health personnel.