Full
Outline
To investigate the role and mechanism of FABP5 in pulmonary inflammation associated with silicosis by analyzing multi-omics data in conjunction with a silicosis mouse model.
Single-cell sequencing was performed to analyze the potential role of FABP5 in occupational respiratory diseases; a silicosis mouse model was used to validate the relationship between FABP5 and pulmonary inflammation in silicosis; intra-abdominal injection of FABP5 inhibitors to modulate FABP5 function; and employed hematoxylin-eosin staining, immunohistochemistry, Western blotting, real-time quantitative PCR, and transcriptomic sequencing to investigate the role and potential mechanisms of FABP5 in silica-induced lung tissue inflammation in mice.
Single-cell sequencing revealed that FABP5 expression was elevated by 3.10-fold (P<0.01) and 4.71-fold (P<0.05) in lung tissue from patients with pneumoconiosis and in a mouse model of silicosis, respectively, and was highly enriched in macrophages; Transcriptomic sequencing revealed that, compared to the silicosis model group, inhibition of FABP5 downregulated the expression of inflammatory genes such as TNF, IL-1α, and IL-1β, with the relative protein expression levels of TNF-α and IL-1β decreasing by 4.67-fold (P<0.001) and 0.25-fold (P<0.01), respectively.
FABP5 is a key pathogenic factor in silicosis-induced pulmonary inflammation. In vivo inhibition of FABP5 alleviates pulmonary tissue inflammation in silicosis, providing insights for research into the mechanisms and clinical management of silicosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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