To explore the clinical efficacy and safety of Simo Decoction Oral Liquid（四磨汤口服液）in the treatment of functional constipation（Qi stagnation constipation）.

A multicenter, randomized, double-blind and placebocontrolled design was used to randomly divide 80 patients with functional constipation（Qi stagnation constipation）in 7 hospitals from March 2022 to June 2023 into two groups, 40 cases in the experimental group were given oral administration of Simo Decoction Oral Liquid, and 40 cases in the control group were given Simo Decoction Oral Liquid simulant. Both groups took the medicine two sticks for each time, twice a day, and the course was 8 weeks. The response rate of complete spontaneous defecation（CSBM）, the improvement of bowel movement - related symptoms, the scores of TCMsyndromes, the number of emergency drugs taken and the adverse reactions of the two groups were observed.

The overall CSBM response rate in the experimental group（FAS: 77.50%；PPS: 83.80%）was better than that in the control group（FAS: 22.50%；PPS: 17.10%）, with a statistically significant difference（P<0.001）. The weekly CSBMresponse rate of the experimental group（FAS: 82.5%）was significantly better than that of the control group（FAS: 22.50%）, with a statistically significant difference（P<0.001）. The change value of CSBMin the experimental group（FAS: 2.95±1.79）was higher than that of the control group（FAS: 0.74±1.53）, and the difference was statistically significant（P<0.001）. The abdominal pain in defecation, abdominal distension, effortful defecation and medication assisted defecation frequency, the primary symptoms（abdominal distension and unpleasant defecation）and the secondary symptoms（intestinal sounding and fullness in the chest and ribs points）of TCMsyndromes, as well as the amount of emergency medication intake before and after the treatment in the experimental group were better than those in the control group, with a statistically significant difference（P<0.05）. No adverse reactions related to the study drug occurred in neither group, the incidence of adverse reactions was 0.

The total CSBMresponse rate of functional constipation（Qi stagnation constipation）treated with Simo Tang Oral Liquid was more than 50% higher than that of the control group, and it significantly improved the bowel movement-related symptoms such as abdominal pain, abdominal distention, unpleasant defecation, bowel sound, and chest and rib tightness, and the medication was safe.

To investigate the regulation effect of astragalus polysaccharide on lung cancer-bearing mice in vivo immunity and tumor cell growth.

A total of 72 SPF-grade C57BL/6J mice were randomly divided into a model group（treated with an equal volume of normal saline by gavage）, a positive control group（treated with cisplatin 6.0 mg/kg via intraperitoneal injection）, a low-dose group（treated with astragalus polysaccharide solution 50 mg/kg by gavage, at a concentration of 1.0 mg/mL）and a high-dose group（treated with astragalus polysaccharide solution 100 mg/kg by gavage, at a concentration of 1.0 mg/mL）, with 18 mice in each group. The tumor volume, tumor mass, tumor inhibition rate, T lymphocyte count in peripheral blood and Survivin protein expression in tumor tissues were compared among the groups 14 days after modeling. Flow cytometry was used to detect the staging distribution of tumor cells in the tumor tissues of mice in each group.

Compared with those in the model group, the tumor volume and tumor mass in the positive control, low-dose and high-dose groups were all significantly lower（P<0.05）. Specifically, the tumor volume and tumor mass in the positive control group were significantly lower than those in the low-dose and high-dose groups（P<0.05）, while the tumor inhibition rate was significantly higher（P<0.05）. Furthermore, the tumor volume and tumor mass in the high-dose group were lower than those in the low-dose group（P<0.05）, and the tumor inhibition rate was higher（P<0.05）. In terms of peripheral blood, the levels of CD4⁺ and CD4⁺/CD8⁺ in the low-dose and high-dose groups were higher than those in the model and positive control groups（P<0.05）, while the CD8⁺ level was lower（P<0.05）. Additionally, the levels of CD4⁺ and CD4⁺/CD8⁺ in the high-dose group were higher than those in the low-dose group（P<0.05）, and the CD8⁺ level was lower（P<0.05）. Regarding Survivin protein expression in tumor tissues, the scores in the positive control, low-dose and high-dose groups were all lower than those in the model group（P<0.05）. Notably, the Survivin protein expression in the positive control group was lower than that in the low-dose and high-dose groups（P<0.05）, and the expression in the high-dose group was lower than that in the low-dose group（P<0.05）. Finally, the proportions of S-phase cells in tumor tissues were higher in the positive control, low-dose and high-dose groups compared to those in the model group（P<0.05）. Specifically, the proportion of S-phase cells in the positive control group was higher than that in the low-dose and high-dose groups（P<0.05）, and the proportion in the high-dose group was higher than that in the low-dose group（P<0.05）.

Astragalus polysaccharide can regulate the immune function and reduce the expression of Survivin protein in the treatment of lung cancer-bearing mice, thereby inhibiting the growth of tumor cells.

To study the clinical observation on the treatment of cervical spondylosis by acupuncture and moxibustion combined with Modified Guizhi Jia Gegen Decoction（桂枝加葛根汤加减）guided by the meridian tendons theory.

Eighty-two patients diagnosed as cervical spondylosis were selected and divided into two groups according to the random number table. Forty-one cases in the reference group were treated with Modified Guizhi Jia Gegen Decoction, and forty-one cases in the study group were treated with acupuncture and moxibustion and Modified Guizhi Jia Gegen Decoction based on the theory of meridian tendons. The clinical efficacy, traditional Chinese medicine（TCM）syndrome scores, scales scores and inflammatory indexes of the two groups were observed and compared.

Compared with that of the reference group, the total effective rate of the study group was 97.56%（40/41）, higher than that（75.61%, 31/41）of the reference group, and the difference was statistically significant（P<0.05）. After treatment, the TCMsyndrome scores of the study group were lower than those of the reference group, and the difference was statistically significant（P<0.05）. After treatment, compared with those of the reference group, the pain visual analogue scale（VAS）and cervical dysfunction index scale（NDI）were lower in the study group, and the joint motion rating（ROM）and manual muscle strength rating（MMT）were higher in the study group, with statistical significance（P<0.05）. After treatment, the levels of C-reactive protein（CRP）and erythrocyte sedimentation rate（ESR）in the study group were lower than those in the reference group, and the difference was statistically significant（P<0.05）.

For patients with cervical spondylosis, acupuncture and moxibustion guided by the theory of meridian tendons combined with Modified Guizhi Jia Gegen Decoction can effectively improve the therapeutic effect, relieve the clinical symptoms, reduce the inflammatory response and promote the recovery of the patients, with high clinical application value.

To study the effect of benefiting Qi and elevating Zong method in the treatment of heart failure with preserved ejection fraction（HFpEF）.

From December 2019 to May 2021, 60 patients with HFpEF treated in Chongqing Hospital of Traditional Chinese Medicine were selected as the research objects. They were divided into two groups by random number grouping method, 30 patients in the control group and 30 patients in the observation group. The patients in the control group were treated with standardized heart failure drugs, and those in the observation group were treated with Yiqi Shengzong Decoction（益气升宗汤）on the basis of the control group. After 4 weeks of treatment, the syndrome efficacy, cardiac structure and cardiac function indexes, vascular inflammation indexes, quality of life and incidence of adverse reactions were compared between the two groups.

The curative effect of the observation group was better than that of the control group, and the total effective rate was 96.67%（29/30）vs 73.33%（22/30）（P<0.05）. The left atrial volume index（LAVI）, left ventricular mass index（LVMI）, early diastolic mitral flow velocity/early diastolic mitral annular velocity（E/e＇）and tricuspid regurgitation peak velocity（TRV）were significantly decreased in the two groups. Compared with those of the control group, the values of each index in the observation group were lower（P<0.05）. After treatment, the 6-minute walking test（6MWT）distance of the two groups increased, and the 6MWT of the observation group was longer than that of the control group（P<0.05）. After treatment, the levels of serum C-reactive protein（CRP）and soluble growth stimulation expressed gene 2（sST2）in the two groups were significantly decreased, and those in the observation group were significantly lower than those of the control group（P<0.05）. After treatment, the scores of Minnesota Heart Failure Quality of Life Scale（MLHFQ）in the two groups were significantly decreased, and those in the observation group were significantly lower than the control group（P<0.05）. No serious adverse reactions occurred in both groups.

The treatment of HFpEF with benefiting Qi and elevating Zong method is satisfactory, and the curative effect of syndrome and NYHA cardiac function is better. It can significantly improve the cardiac structure and cardiac function index, vascular inflammation index and the quality of life. It is an effective and safe medication regimen and is worthy of application.

To investigate the clinical effect of Liujunzi Decoction（六君子汤）in the adjuvant treatment of children with bronchial asthma, and its effects on intestinal flora, corticotropin, corticosterone and microflora.

A total of 116 children with bronchial asthma admitted to the Department of Pediatrics of Jurong Hospital Affiliated to Jiangsu University from November 2022 to June 2024 were selected as the study objects. The patients were divided into two groups by random number table method, and 58 patients in the control group received conventional Western medicine treatment and those（58 patients）in the experimental group were treated with Liujunzi Decoction on the basis of conventional western medicine, all of whom were treated for 4 weeks. After treatment, the clinical effects, TCMsyndrome scores, intestinal flora, adrenocorticotropin and corticosterone before and after treatment were observed and compared between the two groups.

Compared with that（82.76%）of the control group, the overall curative effect was 96.55%（P<0.05）. Compared with those of the control group, the TCMsyndrome scores of the experimental group decreased more significantly（P<0.05）. Compared with those of the control group, the levels of corticotropin and corticosterone in the experimental group were lower（P<0.05）. Compared with those of the control group, bifidobacterium and lactobacillus in the experimental group were higher, and Escherichia coli was lower（P<0.05）. The detection rates of Staphylococcus aureus and Streptococcus virescens in the experimental group were lower than those in the control group（P<0.05）, and the detection rates of other bacteria groups were not statistically different between the two groups（P>0.05）.

After the adjuvant treatment of children with bronchial asthma with Liujunzi Decoction, the therapeutic effect is significantly improved, which can effectively improve the imbalance of intestinal flora and promote the recovery of children. It provides solid theoretical support for the clinical application of formulas and fosters the inheritance and innovation of traditional Chinese medicine.

To explore the effect of Guizhi Shaoyao Zhimu Decoction（桂枝芍药知母汤）combined with adalimumab on inflammatory response and blood viscosity in rheumatoid arthritis（RA）.

Seventy-two patients with RA of windcold-dampness type admitted to Shandong Wendeng Orthopedic Hospital from January 2021 to December 2023 were enrolled for the research and randomly divided into the control group（adalimumab treatment, 36 cases）and the experimental group（Modified Guizhi Shaoyao Zhimu Decoction combined with adalimumab treatment, 36 cases）. The blood levels of inflammatory indicators[tumor necrosis factor-α（TNF-α）, C-reactive protein（CRP）, interleukin-6（IL-6）, nod-like receptor protein3（NLRP3）], blood viscosity related indicators[whole blood viscosity-high shear, whole blood viscosity-low shear, plasma viscosity, fibrinogen（Fib）], traditional Chinese medicine syndrome scores, disease activity scale-28（DAS-28）, World Health Organization Quality of Life Test-Bref（WHOQOL-BREF）scores were compared between the experimental group and the control group before and 12 weeks after treatment.

There were no statistically significant differences in the general information and various indicators between the experimental group and the control group of RA patients before treatment（P>0.05）. Compared with those before treatment within the group, the blood levels of TNF-α, CRP, IL-6, NLRP3, whole blood viscosity-high shear, whole blood viscosity-low shear, plasma viscosity and Fib, traditional Chinese medicine syndrome score and DAS-28 score in two groups were significantly reduced, while the WHOQOL-BREF total score was significantly increased（P<0.05）. The blood levels of TNF-α, CRP, IL-6, NLRP3, whole blood viscosity-high shear, whole blood viscosity-low shear, plasma viscosity, Fib, Traditional Chinese Medicine syndrome score and DAS-28 score after treatment in experimental group were all significantly lower than those in the control group, while the total score of WHOQOL-BREF was significantly higher than that in the control group（P<0.05）.

Guizhi Shaoyao Zhimu Decoction combined with adalimumab treatment could significantly alleviate the inflammation and blood viscosity in RA patients with wind-cold-dampness type, improve traditional Chinese medicine syndromes, reduce the disease activity and improve the quality of life, which is worthy of promoting and applying.

To analyze the effects of Modified Buyang Huanwu Decoction（补阳还五汤加减）combined with consciousness-restoring and orifice-opening acupuncture on hemorheology and serum levels of interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α） in patients with ischemic stroke in the convalescent period（Qi deficiency syndrome）.

From April 2022 to March 2024, 80 patients with ischemic stroke in the convalescent period who were admitted to rehabilitation medicine department of the hospital were selected and divided into the control group（4 weeks of conventional treatment with Modified Buyang Huanwu Decoction）and the observation group（4 weeks of combined treatment with Modified Buyang Huanwu Decoction and consciousness-restoring and orifice-opening acupuncture）using the random number table method, with 40 cases in each group. The TCMclinical effect, hemorheology[whole blood high-shear viscosity, whole blood low-shear viscosity, plasma viscosity and fibrinogen（FIB）], neurological function indicators[the National Institutes of Health Stroke Scale（NIHSS）, nerve growth factor（NGF）and brain-derived neurotrophic factor（BDNF）], serum levels of IL-6 and TNF-α, quality of life[Short Form 36（SF-36）]and the incidence of adverse reactions were compared between the groups.

The effective rate of treatment in the observation group（92.50%, 37/40）was higher than that in the control group（75.00%, 30/40）（P<0.05）. After treatment, the levels of whole blood high-shear viscosity, whole blood low-shear viscosity, plasma viscosity and FIB in the observation group were lower than those in the control group（P<0.05）. After treatment, NIHSS score of the observation group was lower than that of the control group, and the levels of NGF and BDNF were higher than those of the control group（P<0.05）. The serum levels of IL-6 and TNF-αin the observation group after 2 and 4 weeks of treatment were lower than those in the control group, while SF-36 scores were higher than those of the control group（P<0.05）. The incidence rates of adverse reactions in the two groups were 12.50%（5/40）and 10.00%（4/40）, without statistically significant difference between two groups（P>0.05）.

Applying Modified Buyang Huanwu Decoction combined with consciousness-restoring and orifice-opening acupuncture in the treatment of patients with Qi deficiency type of ischemic stroke in the convalescent period can significantly improve the clinical effect, hemorheology, serum levels of IL-6 and TNF-α, neurological function and quality of life. It is safe and reliable.

To investigate the effects of Shuangshen Decoction（双肾汤）on inflammatory factors, immune function and recurrence in children with purpura nephritis.

A hundred cases with purpura nephritis were randomly divided into the control group and the observation group, 50 cases in each group. Both groups were given glucocorticoid therapy, and the observation group was given Shuangshen Decoction simultaneously. The changes of syndrome scores, serum inflammatory factors and immune function were observed before and 12 weeks after treatment, and the recurrence of infection was followed up within 12 months.

There was no statistically significant difference in the scores of traditional Chinese medicine symptoms between the two groups before treatment（P>0.05）. The scores decreased at after treatment（P<0.05）and the decline degree in the observation group was greater than that in the control group（P<0.05）. There was no statistically significant difference in the serum tumor necrosis factor-α（TNF-α）, interleukin（IL）-6 or IL-10 between the two groups before treatment（P>0.05）. There were decreased after treatment（P<0.05）and the decline degree in the observation group was greater than that in the control group（P<0.05）. There was no statistically significant difference in the CD4⁺, CD8⁺, IgA, IgG or IgM between the two groups before treatment（P>0.05）. The levels of CD4⁺, IgA and IgG in the observation group were higher after treatment than those before treatment（P<0.05）, and it was higher than that of the control group after treatment（P<0.05）. The recurrence rate and infection rate of the observation group were 22.00%（11/50）and 34.00%（17/50）, which were lower than those of the control group[44.00%（22/50）and 62.00%（31/50）]（P<0.05）. The number of recurrence and infection in the observation group was lower than that in the control group（P<0.05）, and the therapeutic effect in the observation group was higher than that in the control group（P<0.05）.

Shuangshen Decoction in the treatment of children with purpura nephritis can help to alleviate symptoms, improve immune function and inflammatory response, and reduce infection and recurrence rate.

To observe the efficacy of Bailing Tablets（百令片）combined with conventional western medicine on connective tissue disease-related interstitial lung disease（CTD-ILD）.

Sixty-seven patients with CTD-ILD from 6 hospitals were included for analysis. All patients received conventional western medicine treatment. They were divided into observation group（36 cases）and control group（31 cases）according to their preference. The observation group was additionally administered with Bailing Tablets, 5 tablets for each time, 3 times per day. Two groups were treated for 6 months. The follow-up time was 3 and 6 months after enrollment. The outcomes were lung function, traditional Chinese medicine（TCM）syndrome scores, St. George＇s Respiratory Questionnaire（SGRQ）scores, 6-minute walk test distance（6MWT）and humoral immune indicators. Safety evaluation data was also recorded.

After treatment, the forced vital capacity（FVC）showed an improving trend in the observation group[（0.38±0.75）L], which was statistically significant compared with that in the control group[（-0.22±0.76）L]（P<0.05）. Compared with the control group, the observation group had significantly higher levels of improvement difference in percentage of forced vital capacity to predicted value（FVC% pre）, percentage of forced expiratory volume in the first second to predicted value（FEV1% pre）and forced expiratory volume in the first second（FEV1）（P<0.05）. The observation group showed better improvement in TCMsyndrome scores, SGRQ scores and 6MWT compared to the control group, and had lower levels of IgG and IgM, with statistically significant differences（P<0.05）. No obvious adverse reactions were observed in either group.

Based on the combination of conventional western medicine treatment, Bailing Tablets can improve the clinical symptoms and lung function of CTD-ILD, regulate the immune function, and have high safety.

To observe the curative effect of Shunao Jieyu Decoction（疏脑解郁汤）combined with moxibustion of regulating governor and relieving depression-transcranial magnetic stimulation（rTMS）on the rehabilitation of the patients with post-stroke depression（PSD）.

A total of PSD patients admitted to Hebei Provincial Hospital of Traditional Chinese Medicine from June 2021 to January 2023 were randomly divided into the combined group（60 cases）and the control group（60 cases）. Both groups were treated with Escitalopram Oxalate Tablets and the moxibustion method of regulating Du meridian and relieving depression-rTMS, and the combined group was intervened with Shunao Jieyu Decoction for 4 weeks. The changes of Hamilton Depression Scale（HAMD）, TCMsyndrome scores and modified Edinburgh-Scandinavian stroke scale（MESSS）before and after treatment were compared between the two groups. The side effects of the two groups were evaluated by Asperger＇s anti-depressant side effects scale, and the therapeutic effects of the two groups were evaluated after treatment.

After 2 and 4 weeks of treatment, the HAMD scores, TCMsyndrome scores and MESSS scale scores of the two groups decreased compared with those before treatment, while the scores of the combined group were lower than those of the control group after 2 and 4 weeks of treatment（P<0.05）. The incidence of side effects in the combined group and the control group were 15.00%（9/60）and 18.33%（11/60）, respectively, and there was no statistical difference between the two groups（P>0.05）. The total effective rate of the combined group was 91.67%（55/60）, which was significantly higher than that of the control group（76.67%, 46/60）（P<0.05）.

On the basis of regulating Du and relieving depression moxibustion-rTMS therapy, the combined application of Shunao Jieyu Decoction can effectively regulate the depression, relieve the clinical symptoms and improve the neurological function without affecting the safety of treatment, so as to achieve a more ideal therapeutic effect.

To explore the effects of a multimodal analgesia regimen combining pre-treatment with acetaminophen mannitol injection, acupuncture and transversus abdominis plane block（TAPB）on postoperative recovery in elderly patients after laparoscopic radical gastrectomy.

This study utilized a randomized controlled trial design, enrolling the elderly patients undergoing radical gastrectomy who were randomly assigned into three groups: the control group（TAPB group）, the acetaminophen +TAPB group, and the acupuncture +acetaminophen + TAPB group. Pretreatment with paracetamol mannitol injection was given before operation, and TAPB, acupuncture and moxibustion and combined treatment were performed after operation. The vital signs, pain scores, analgesic drug usage, postoperative recovery and incidence of adverse events within 48 hours after surgery were compared among the three groups.

Compared to the TAPB group, the acupuncture +acetaminophen + TAPB group demonstrated significant differences in postoperative heart rate recovery, blood pressure stability, control of visual analogue scale（VAS）scores, reduced postoperative analgesic consumption, earlier time to first ambulation, shortened time to first flatus and decreased incidence of postoperative delirium and shoulder pain（P<0.05）. The acetaminophen + TAPB group also showed advantages over the TAPB group in some indicators, but not as significantly as the acupuncture + acetaminophen + TAPB group.

The multimodal analgesia regimen of pretreatment with acetaminophen mannitol injection combined with acupuncture and TAPB significantly improves postoperative recovery in the elderly patients after laparoscopic radical gastrectomy. It effectively controls the postoperative pain, reduces the analgesic use, promotes the gastrointestinal function recovery and decreases the incidence of postoperative adverse events.

To observe the effects of Bushen Huoxue Formula（补肾活血方）combined with enema treatment on hormone, inflammation levels and pregnancy outcomes in patients with chronic endometritis complicated with infertility.

A prospective study was conducted on 102 patients with chronic endometritis who received treatment at Hengshui People＇s Hospital from July 2022 to March 2024.. The patients were divided into a control group and an observation group according to the random number table method, with 51 patients in each group. The control group received Western medicine anti infection treatment, while the observation group was treated with a combination of Bushen Huoxue Formula orally and traditional Chinese medicine enema for three consecutive menstrual cycles. The traditional Chinese medicine syndrome scores, traditional Chinese medicine syndrome efficacy, serum sex hormone and inflammation levels, pregnancy outcomes and adverse reactions were compared between two groups.

The total effective rate of the observation group（96.08%, 49/51）was significantly better than that of the control group（84.31%, 43/51）, and the difference was statistically significant（P<0.05）. Before intervention, there was no significant difference in the TCMsyndrome scores of the main or secondary symptoms between the two groups（P>0.05）. After intervention, compared with those of the control group, the TCMsyndrome scores of the main and secondary symptoms in the observation group were significantly down-regulated（P<0.01）. There was no significant difference in the levels of progesterone（P）, estradiol 2（E2）or follicle stimulating hormone（FSH）between the two groups before intervention（P>0.05）. After intervention, the levels of P and FSH were down-regulated and the E2 levels up-regulated in the two groups and the changes of the observation group was more obvious（P<0.01）. After intervention, the levels of interleukin-1 β（IL-1 β）, interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α）in the observation group were significantly down-regulated（P<0.01）. The incidence of adverse reactions in the observation group（15.68%, 8/51）showed no significant difference compared to that in the control group（9.80%, 5/51）（P>0.05）. Compared with those of the control group, the clinical pregnancy rate and embryo implantation rate of the observation group were significantly up-regulated（P<0.05）, while there was no significant difference in ectopic pregnancy rate, miscarriage rate or preterm birth rate between the two groups（P>0.05）.

The combination of Bushen Huoxue Formula and enema can significantly enhance the therapeutic effect, alleviate the clinical symptoms such as abdominal pain and menstrual disorders, regulate the hormone levels, inhibit the inflammatory reactions and improve the pregnancy outcomes of infertile patients with chronic endometritis.

To observe the effect of Zuojin Jiawei Prescription（左金加味方）combined with dexzopiclone in the treatment of gastroesophageal reflux disease（liver-stomach stagnation heat type）with insomnia.

A total of 68 patients with gastroesophageal reflux disease（liver-stomach stagnation heat type）with insomnia admitted from June 2021 to January 2024 were included in the study. The patients were divided into two groups by random number table method, namely control group and observation group, with 34 cases in each group. All patients in the group received conventional Western medicine treatment, while the control group received dexzopiclone treatment. The observation group was treated with Zuojin Jiawei Prescription on the basis of control group, and the data of patients in each group was observed and compared, including clinical efficacy, changes of TCMsyndrome scores（acid reflux, heartburn, belching or regurgitation, epigastric burning pain, etc.）before and after treatment, endoscopic esophageal mucosa score and reflux diagnostic questionnaire changes in RDQ scores, the changes in Pittsburgh sleep quality index（PSQI）and short form 36 questionnaire（SF-36）scores and adverse reactions.

The total effective rate of the observation group was higher than that of thee control group（P<0.05）. The adverse reaction rate was lower in both groups（P>0.05）. Comparison of TCMsyndrome scores（acid reflux, heartburn, belching or regurgitation, epigastric burning pain, etc）, endoscopic esophageal mucosa score, RDQ score, PSQI score and SF-36 score in both groups before treatment was not significant（P>0.05）. After treatment, TCM syndrome scores（acid reflux, heartburn, belching or regurgitation, epigastric burning pain, etc.）, endoscopic esophageal mucosa scores, RDQ scores, PSQI scores and SF-36 scores were all improved in both groups. And these indexes in the observation group were all better than those in the control group（P<0.05）.

Zuojin Jiawei Prescription combined with dexzopiclone has a good effect in the treatment of gastroesophageal reflux disease（liver-stomach stagnation heat type）with insomnia, and can improve the clinical symptoms, sleep quality and quality of life of patients, which is worthy of application.

To study the regulatory effect of tripterygium glycosides（TG）on ferroptosis of osteosarcoma cells via transcription factor EB（TFEB）-lysosome pathway.

Osteosarcoma U2OS cells were cultured and divided into control group, TG groups with different concentrations（10, 20, 40, 80, 160, 320 μmol/L）, negative control（NC）group（transfected with NC siRNA）, and NC + TG group（transfected with NC siRNA combined with 160 μmol/L TG）, si-TFEB + TG（transfected with TFEB siRNA combined with 160 μmol/L TG）. Cell proliferation activity was detected by CCK-8 assay, lysosome probe Lyso-Tracker activity was detected by flow cytometry, Fe²⁺ and reactive oxygen species（ROS）levels were detected by enzymolysis assay. Western blot analysis was performed to detect the expression levels of TFEB in total protein and nuclear protein and the expression levels of lysosome related proteins（LAMP1, LAMP2, CTSB, CTSD）and ferroptosis related proteins（GPX4, SLC7A11）in total protein.

The survival rate of TG groups with different concentrations was lower than that of the control group（P<0.05）, and the higher the concentration of TG, the lower the survival rate. The LysoTracker activity and the expression levels of LAMP1, LAMP2, GPX4 and SLC7A11 in total protein of low, medium and high concentration TG groups（40, 80, 160 μmol/L） were lower than those in the control group, the expression levels of Fe²⁺, ROS and TFEB in nuclear protein, the expressions of CTSB and CTSD in total protein were higher than those in the control group（P<0.05）. The survival rate, LysoTracker activity and the expression levels of LAMP1, LAMP2, GPX4 and SLC7A11 in total protein of si-TFEB + TG group were higher than those in the NC + TG group, the expression levels of Fe²⁺, ROS and TFEB in nuclear protein, the expression of CTSB and CTSD in total protein were lower than those in the NC + TG group（P<0.05）.

TG affects lysosomal function by promating TFEB nuclear translocation, and then promotes ferroptosis in osteosarcoma cells.

To study the clinical study of Biejiajian Pills（鳖甲煎丸）treating hepatitis B liver fibrosis（blood stasis in liver and spleen syndrome）.

A total of 102 patients with hepatitis B liver fibrosis（blood stasis in liver and spleen syndrome）admitted to the hospital from November 2022 to July 2024 were selected as the study objects and the patients were divided into two groups by random number table method. Fifty-one patients receiving conventional Western medicine were established as the control group and fifty-one patients receiving Biejiajian Pills on the basis of the conventional Western medicine were established as the experimental group. After treatment, the clinical effects of the two groups were observed and compared, including TCMsyndrome scores, hepatitis B virus e antigen（HBeAg）negative conversion rate, hepatitis B virus e antibody（HBe-Ab）positive conversion rate, liver function, liver fiber and inflammatory indicators before and after treatment.

After treatment, the TCMsyndrome scores of both groups decreased, and the scores of the experimental group decreased more significantly than that of the control group（P<0.05）. Compared with those in the control group, the levels of glutalanine transferase（ALT）, glutamic oxalate transferase（AST）and total bilirubin（TBIL）in the experimental group were lower, and the proportion of white ball（A/G）was higher（P<0.05）. Compared with those in the control group, the levels of laminin（LN）, hyaluronic acid（HA）, type Ⅲ procollagen（PC-Ⅲ）and type Ⅳ collagen（C-Ⅳ）in the experimental group were lower（P<0.05）. The conversion rate of HBeAg to negative was 49.02%（25/51）, and the conversion rate of HBeAb to positive was 41.18%（21/51）in the experimental group, which were higher than 27.45%（14/51）and 19.61%（10/51）in the control group（P<0.05）. Compared with those in the control group, the levels of interleukin-15（IL-15）, interleukin-16（IL-16）and transforming growth factor-β1（TGF-β1）in the experimental group were lower（P<0.05）.

Biejiajian Pills can effectively improve the liver function and liver fiber index, reduce the clinical symptoms and control the condition of patients with hepatitis B liver fibrosis（blood stasis in liver and spleen syndrome）, and has high clinical application value.

To observe the effect of Bushen Huoxue Formula（补肾活血方）on myocardial fibrosis and TGF-β1/Smads signaling pathway in rats with heart failure after myocardial infarction.

120 male SD rats aged 12 weeks were purchased and 20 were selected as the sham surgery group using the random number method. The remaining 100 rats underwent anterior descending coronary artery ligation, combined with starvation and exhaustive swimming to create a heart failure model after myocardial infarction. A total of 80 model rats were randomly divided into a model group, a Western medicine group, a Chinese medicine high dose group, a Chinese medicine medium dose group and a Chinese medicine low dose group. The rats in the Chinese medicine high, medium and low dose groups were given Bushen Huoxue Formula by gavage at a concentration ratio of 4∶2∶1. The western medicine group was given a suspension of sacubitril and valsartan sodium tablets by gavage. The model group and sham surgery group were given an equal amount of distilled water by gavage. After 4 weeks of continuous medication, ultrasound was used to detect the left ventricular end systolic diameter（LVESD）, left ventricular end diastolic diameter（LVEDD）, left ventricular ejection fraction（EF）and left ventricular short axis shortening rate（FS）in six groups. Masson staining was used to observe the degree of myocardial fibrosis in the six groups and the collagen volume fraction（CVF）was measured. Western blotting（WB）was used to measure the protein expression levels of TGF-β1, p-Smad2, p-Smad3, Collagen Ⅰ and Collagen Ⅲ in the myocardial tissue of the six groups. Fluorescence quantitative polymerase chain reaction（PCR）was used to detect the mRNA expressions of Smad6 and Smad7 in the myocardial tissue of the six groups.

After 4 weeks of medication, compared with those of the sham surgery group, the levels of LVESD and LVEDD of the model group increased, while the levels of EF and FS decreased. The myocardial structure was disordered, CVF increased, and the protein expressions of TGF-β1, p-Smad2, p-Smad3, Collagen Ⅰ and Collagen Ⅲ in myocardial tissue increased. The mRNA expressions of Smad6 and Smad7 decreased（P<0.01）. Compared with those of the model group, the levels of LVESD and LVEDD of rats in the western medicine group and the Chinese medicine low, medium and high dose groups were reduced, while the levels of EF and FS were increased. The myocardial structure was neat, CVF was reduced, and the protein expressions of TGF-β1, p-Smad2, p-Smad3, Collagen Ⅰ and Collagen Ⅲ in myocardial tissue was reduced. The expressions of Smad6 mRNA and Smad7 mRNA were increased（P<0.05 or P<0.01）. Compared with the Chinese medicine high dose group, the Chinese medicine medium dose group showed an increase in the levels of LVESD and LVEDD, a decrease in the levels of EF and FS, an increase in the protein expressions of TGF-β1, p-Smad2, p-Smad3, Collagen Ⅰ and Collagen Ⅲ in myocardial tissue, and a decrease in mRNA expressions of Smad6 and Smad7（P<0.01）. Compared with the Chinese medicine medium dose group, the Chinese medicine low dose group showed an increase in the levels of LVESD and LVEDD, a decrease in the levels of EF and FS, an increase in the protein expressions of TGF-β1, p-Smad2, p-Smad3, Collagen Ⅰ and Collagen Ⅲ in myocardial tissue, and a decrease in mRNA expressions of Smad6 and Smad7（P<0.01 or P<0.05）.

Bushen Huoxue Formula can improve myocardial fibrosis in rats with heart failure after myocardial infarction, and its mechanism of action may be related to the inhibition of TGF-β1/Smads activation by Bushen Huoxue Formula.

To observe the anti-inflammatory effect of Fuyanshu Capsule（妇炎舒胶囊, FYS）on sequelae of pelvic inflammatory disease（SPID）model rats on Krüppel-like factor 4（KLF4）, Toll-like receptor 2（TLR2）, nuclear factor-kappa B（NF-κB）and myeloid differentiation factor 88（MyD88）.

Thirty-six rats were randomly divided into blank group, model group, Kangfuyan Capsule（康妇炎胶囊）group（KFY group）, FYS high-dose group, FYS medium-dose group, FYS low-dose group. The SPID animal model was established by the method of mixed bacterial liquid injection and mechanical injury. The blank group was treated with distilled water by gavage, and the other groups were given the corresponding drug suspension by gavage for 21 days. HE staining was used to observe the morphology of uterine tissue and score the pathological sections. The mRNA expressions of TLR2, MyD88 and KLF4 were determined by RT-PCR, the expression of NF-κB was observed by immunohistochemistry method, and the relative protein expressions of TLR2, MyD88 and KLF4 in uterine tissues of each group were determined by Western Blot. The correlation between KLF4 and key factors of TLR2/MyD88/NF-κB pathway was analyzed.

Compared with those in the model group, the scores of uterine tissue inflammation in KFY group, FYS medium - and high-dose groups were significantly decreased（P<0.05）. KLF4 protein in KFY group and FYS high-dose group was significantly increased（P<0.01）. The protein and mRNA of TLR2 in KFY group and FYS high-dose group were significantly decreased（P<0.05）. MyD88 protein in KFY group, FYS high-and medium-dose groups was significantly decreased（P<0.05）, and MyD88 mRNA in KFY group and FYS high-dose group was significantly decreased（P<0.05）. The expressions of NF-κB protein in KFY group, FYS medium - and high-dose groups were significantly decreased（P<0.05）. The expression of KLF4 protein was negatively correlated with the expressions of TLR2 protein, MyD88 protein and NF-κB protein（P<0.05）.

FYS can inhibit the TLR2/MyD88/NF-κB pathway by up-regulating KLF4 expression.

To investigate the mechanism of action of Bufei Decoction（补肺汤）in intervening chronic obstructive pulmonary disease（COPD）lung Qi deficiency by modulating the levels of mitochondrial autophagy mediated by phosphatase and tensin homolog-induced putative kinase protein 1（PINK1）/RBR domain E3 ubiquitin protein ligase（Parkin）signaling path-way.

The rats were randomly divided into the control group and the modeling group, and a rat model of COPD lung Qi deficiency was created by using passive smoke inhalation combined with intra-airway lipopolysaccharide drip. At the end of modeling, the rats in the modeling group were randomly divided into the model group, Bufei Decoction medium-dose group, Bufei Decoction high-dose group and 3-methyladenine（3-MA）group. The Bufei Decoction medium - and high-dose groups were given Bufei Decoction drug solution by gavage（7.29, 14.58 g·kg^-1）, and 3-MA group was given 3-MA（15 mg·kg^-1）by intraperitoneal injection, and each group was intervened with the drug once daily for 28 d consecutively. Hematoxylineosin（HE）staining was used to observe the pathological changes in lung tissues. The transmission electron microscopy was used to observe ultrastructural changes in alveolar type Ⅱ epithelial cells in lung tissues. The immunohistochemistry（IHC）method was used to detect the positive expressions of PINK1, Parkin, autophagy receptor protein P62（sequestosome 1, P62/SQSTM1）and microtubule-associated protein 1 light chain 3（LC3）proteins in lung tissues and protein immunoblotting（Western blot）to detect the expressions of PINK1 in mitochondria in lung tissues, Parkin, P62, LC3Ⅱ/LC3I protein expression in mitochondria of lung tissues. Flow cytometry detected the changes of mitochondrial membrane potential in lung tissues. The reactive oxygen species fluorescent probe dichloro-dihydro-fluorescein diacetate（DCFH-DA）and mitochondrial superoxide（MitoSOX）red fluorescent probe were used to determine the levels of reactive oxygen species（ROS）and mitochondrial reactive oxygen species（mtROS）in lung tissues by flow cytometry, respectively. Western blot detected the expressions of nuclear factor-κB P65（NF-κB P65）and phosphorylation of nuclear factor-κB P65（p-NF-κB P65）proteins in lung tissues.

Compared with that in the control group, the lung tissue damage in the model group was severe. Compared with that in the model group, the pathological damages in the Bufei Decoction medium - and high-dose groups and 3-MA group were improved to different degrees. Compared with those of the control group, the lung tissue of the model group showed severe damage to alveolar type Ⅱ epithelial cells and their mitochondrial structure, with a decrease in mitochondria and an increase in autophagic vesicles. Compared with those of the model group, the alveolar type Ⅱ epithelial cells and their mitochondrial structure of the model group showed a lesser degree of damage in the Bufei Decoction medium - and high-dose groups and the 3-MA group, with a relatively higher increase in mitochondria and a decrease in autophagic vesicles. Compared with those in the control group, the protein expressions of PINK1, Parkin, LC3 and LC3Ⅱ/LC3I were up-regulated（P<0.01）and the protein expression of P62 was down-regulated（P<0.01）in the lung tissues and mitochondria of lung tissue in the model group. Compared with those in the model group, the protein expressions of PINK1, Parkin and LC3Ⅱ/LC3I were up-regulated（P<0.01）and the protein expression of P62 was down-regulated（P<0.01）in the lung tissues and mitochondria of lung tissue in the Bufei Decoction medium - and high-dose groups and 3-MA group. The protein expressions of PINK1, Parkin, LC3 and LC3Ⅱ/LC3I were down-regulated（P<0.05, P<0.01）and P62 protein expression was up-regulated（P<0.01）. Compared with those in the control group, the mitochondrial membrane potential of lung tissues in the model group was decreased（P<0.01）, the levels of ROS and mtROS were up-regulated（P<0.01）, the difference in the expression of NF-κB P65 protein was not statistically significant, and the expression of p-NF-κB P65 protein was up-regulated（P<0.01）. Compared with those of the model group, the mitochondrial membrane potential in the lung tissues of the Bufei Decoction medium - and high-dose groups and the 3-MA group were elevated（P<0.01）, the levels of ROS and mtROS were down-regulated（P<0.01）, and the differences in the expression of NF-κB P65 proteins were not statistically significant, and the expression of p-NF-κB P65 protein was down-regulated（P<0.01）.

Bufei Decoction inhibited lung tissue ROS levels and the activation of NF-κB P65, thereby attenuating the oxidative damage and inflammatory responses in the lung of COPD lung Qi deficiency, and its mechanism of action may be related to the inhibition of the level of mitochondrial autophagy mediated by the PINK1/Parkin signaling pathway.

To explore the effects of Danggui Buxue Decoction（当归补血汤）combined with acupuncture on the levels of blood homocysteine（Hcy）, nerve growth factor（NGF）, insulin-like growth factor-1（IGF-1）and vascular endothelial growth factor（VEGF）and the changes in nerve conduction function in diabetic patients with peripheral neuropathy.

This paper collected 120 diabetic patients with peripheral neuropathy admitted to the hospital between January 2022 and January 2024, and were randomly divided into test group and control group. The control group used conventional Western medicine treatment, and the test group used acupuncture and Danggui Buxue Decoction. TCMsyndrome scores, diabetic peripheral neuropathy scores, diabetic neuropathy score（DNS）, clinical efficacy, serum levels of Hcy, NGF, IGF-1 and VEGF and nerve conduction function were compared.

There was no significant difference in TCMsyndrome scores, DNS, the serum levels of Hcy, NGF, IGF-1 or VEGF, the conduction velocities of median nerve motor conduction（MCV）and tibial nerve sensory conduction（SCV）between the two groups before treatment（P>0.05）. The scores of TCMsyndromes and DNS in both groups were significantly lower after treatment and the scores and DNS in the test group were significantly lower than those in the control group（P<0.05）. The clinical effective rate in the test group was significantly higher than that in the control group（P<0.05）. After treatment, the serum levels of Hcy and VEGF in the two groups were significantly lower than those before treatment, the serum levels of NGF and IGF-1, conduction velocities of MCV and SCV on both sides were significantly higher than those before treatment, and the decrease/increase amplitude in the test group was significantly higher than that in the control group（P<0.05）.

- Acupuncture and Danggui Buxue Decoction have obvious effect and can effectively improve the serum levels of Hcy, NGF, IGF-1 and VEGF and the nerve conduction function of the diabetic patients with peripheral neuropathy.

To investigate the effect of Guben Qingyuan Prescription（固本清源方）on inhibiting cancer-associated fibroblast（CAF） in the development of resistance to enzalutamide.

A tumor-bearing mouse model of enzalutamide-resistant prostate cancer cells was established to observe the effects of Guben Qingyuan Prescription combined with enzalutamide on tumor growth and CAF in tumor tissues. The effects of Guben Qingyuan Prescription on CAF transformation were assessed using immunofluorescence. CAF proliferation was evaluated by the CCK8 assay, and the influence of Guben Qingyuan Prescription on CAF-derived factors was measured via enzyme-linked immunosorbent assay（ELISA）.

The tumor inhibition rate of the Guben Qingyuan Prescription combined with enzalutamide ranged from 29% to 47%. In the medium - and high-dose groups, the combination treatment showed a statistically significant difference compared to the enzalutamide-only group（P<0.01）. Immunofluorescence and immunohistochemistry results demonstrated that the expressions of CAF markers fibroblast activation protein-α（FAP）and α-smooth muscle actin（α-SMA）in tumor tissues of enzalutamide-resistant mice were significantly higher than those in the group inoculated with prostate cancer C4-2B cells. The medium - and high-dose Guben Qingyuan Prescription combined with enzalutamide groups significantly inhibited the expressions of FAP and α-SMA compared to the enzalutamide-only group（P<0.05）. Additionally, immunofluorescence showed that serum containing medium - and high-dose Guben Qingyuan Prescription groups could inhibit the expressions of FAP and α-SMA in vitro. After 48 and 72 hours of treatment, the inhibition rates of the medium - and high-dose Guben Qingyuan Prescription groups significantly increased, with a statistically significant difference compared to the control group（P<0.05）. Further studies revealed that Guben Qingyuan Prescription reduced the concentrations of transforming growth factor-β1（TGF-β1）, tumor necrosis factor-α（TNF-α）, stromal cell-derived factor-1（SDF-1）, Wingless/Integrated-3a（Wnt3a）and matrix metalloproteinase 2（MMP2）in CAF supernatants.

Guben Qingyuan Prescription has a significant effect in delaying resistance to enzalutamide, a novel endocrine therapy for prostate cancer, and its mechanism of action may be related to the inhibition of CAF.

The aim of this study was to investigate the effects of myristicin on the regulation of endonuclease/exonuclease/phosphatase family domain containing 1（EEPD1）expression and its subsequent impact on the proliferation, migration, invasion and apoptosis of pulmonary squamous cell carcinoma.

Molecular docking techniques were employed to analyze the interaction between myristicin and the target protein EEPD1. Immunohistochemistry method was used to assess the levels of EEPD1 protein in lung squamous carcinoma and adjacent paracancerous tissues on tissue chips. The gene expression activity in cell lines was evaluated using real-time quantitative polymerase chain reaction（qRT-PCR）, while the effect of myristicin on the proliferation of lung squamous carcinoma cells KNS-62 and NCI-H226 was assessed with Cell Counting Kit-8（CCK-8）. Scratch assay and Transwell migration and invasion experiments were conducted to examine the impact of myristicin on the migration and invasion capabilities of lung squamous carcinoma cells. Flow cytometry method was utilized to determine the influence of myristicin on the apoptosis of lung squamous carcinoma cells, and Western blot（WB）was employed to analyze the expression of EEPD1 protein in these cells.

Myristicin could form specific binding with the EEPD1 protein. Higher EEPD1 positive signal intensity was observed in lung squamous carcinoma tissue samples compared to adjacent paracancerous tissue control areas. CCK-8 assay results demonstrated that myristicin significantly inhibited the proliferation of lung squamous carcinoma cells. A slower scratch healing rate suggested that myristicin could inhibit the migration of lung squamous carcinoma cells. Transwell assay results similarly showed that myristicin could inhibit the migration and invasion of lung squamous carcinoma cells. Flow cytometry data revealed that myristicin promoted the apoptosis of lung squamous carcinoma cells, while WB analysis indicated that myristicin reduced the expression level of EEPD1 protein.

In vitro experimental evidence supports that myristicin exerts its anticancer effects by down-regulating the expression of EEPD1 protein, specifically manifested in inhibiting the proliferation, migration and invasion of pulmonary squamous cell carcinoma and promoting cell apoptosis.

This study explored the pharmacological effect and mechanism of wogonin on lung metastasis of breast cancer after myocardial infarction, and provided potential drugs and experimental basis for the treatment of lung metastasis of breast cancer.

In the experiment, the mouse model of myocardial infarction and breast cancer was established by 6-7 weeks old female balb/c mice, and the effect of wogonin on lung metastasis of breast cancer after myocardial infarction and macrophages in mouse tumors was detected. THP-1, RAW264.7 and bone marrow-derived macrophages（BMDM）cells were induced in vitro to construct macrophage polarization model, and the effect of wogonin on macrophage polarization and the changes of key proteins regulating polarization were detected.

The results showed that compared with those of the model group, the number of lung tumor metastasis, the weight of breast tumor, cardiac function and cardiac inflammation were significantly reduced, and the expression of M2 macrophages in tumor was significantly reduced in the wogonin group. In vitro experiments showed that wogonin significantly inhibited the M2 polarization of macrophages, reduced the phosphorylation of signal transducer and activator of transcription 6（STAT6）and inhibited the migration of MDA-MB 231 cells.

The above results indicate that wogonin can inhibit the lung metastasis of breast adenocarcinoma after myocardial infarction, and its mechanism may be realized by inhibiting the phosphorylation of STAT6 and then affecting the M2 polarization of macrophages in the tumor.

By analyzing the Fourier Transform Infrared Spectroscopy（FT-IR）spectrum of Xiangfu（Cyperi Rhizoma） from different origins, the main contribution band of the differences among the samples from different regions can be founded, so as to trace the origin of Xiangfu（Cyperi Rhizoma）.

Using FT-IR and combining a variety of chemometric methods, 30 batches of samples from 3 producing areas were analyzed. Then the characteristic band peaks were screened according to the main active components, getting the main difference bands of the samples.

Selecting 991, 1018, 526, 1081, 2930, 932, 1514, 708, 579, 1159, 763, 1461 cm^-1 as the main difference contribution bands, and the results of Hierarchical Cluster Analysis（HCA）, Principal Component Analysis（PCA）and Orthogonal Partial Least Squares Discriminant Analysis（OPLS-DA）could be clustered into three categories.

According to the selected characteristic bands, Xiangfu（Cyperi Rhizoma） from different producing areas can be effectively distinguished. The experimental results also show that the differences of saccharides and acids in Xiangfu（Cyperi Rhizoma）from different producing areas can be focused on.

To explore the volatile oil components and quality differences in color and parts of Tangzhizi（Gardeniae Fructus）. This research provided a reference for evaluating the quality and utilizing of Tangzhizi（Gardeniae Fructus）.

GC-MS combined with retention index（RI）was used to analyze volatile oil components of Tangzhizi（Gardeniae Fructus）. HPLC method detected the contents of Crocetin-Ⅰ, Crocetin-Ⅱ, Crocetin-Ⅳ, Crocetin, Crocetin-Ⅲ（at 440 nm）, Geniposidic Acid, Gardenoside, Genipin-1-b-D-gentiobioside and Geniposide（at 240 nm）. Agilent Extend-C18 column was used as the chromatographic column, with acetonitrile -0.1% formic acid aqueous solution as the mobile phase for gradient elution. Electronic eye technology quantified pericarp and kernel of Tangzhizi（Gardeniae Fructus）based on chromaticity value（L^*, a^*, b^*）. Cluster analysis, Pearson correlation analysis and regression equations were used to analyze the relationship between active ingredients and appearance color of pericarp and kernel of Tangzhizi（Gardeniae Fructus）. OPLS-DA method was used to compare quality differences between peel and kernel to identify quality markers.

A total of 78 chemical constituents were identified in the volatile oil of Gardenia jasminoides, with aldehydes comprising the largest proportion, followed by lipids, and phenols in the lowest amount. The total content of9 chemical constituents in the pericarp and kernel varied significantly, with mass fractions of 10.781% and 20.276% respectively. The total sum of the 9 chemical compositions in the kernel was approximately double that of the pericarp, and the gardenia glycoside content in the kernel was about 9 times higher than that in the pericarp. The correlation and regression analysis results indicated that in the pericarp, in addition to Geniposidic Acid, eight other chemical constituents were positively correlated with a^* and negatively correlated with b^*. Among these, saffron Crocetin-Ⅰ, Crocetin, Gardenoside, Genipin-1-b-D-gentiobioside and Geniposide were positively correlated with L^*. However, the kernel of nine chemical constituents showed a non-significant correlation with L^*. Additionally, a^* was positively correlated with a^* and negatively correlated with b^*, showing a significant regression relationship. OPLS-DA categorized the pericarp and kernel and identified L^*, Geniposide, Gardenoside, Genipin-1-b-D-gentiobioside and Geniposidic Acid as the markers influencing herb quality.

To fully utilize Tangzhizi（Gardeniae Fructus）resources, the research is essential for quality evaluation and development, providing a scientific basis for Tangzhizi（Gardeniae Fructus）.

The study aimed to observe the cardioprotective effects of Yiqi Wenyang Huoxue Lishui Recipe（益气温阳活血利水方, YWHLR）by regulating the mixed lineage kinase 3（MLK3）mediated mitochondrial unfolded protein response（UPR^mt）and to explore the mechanism of YWHLR in preventing and treating heart failure.

The mouse HL-1 cardiomyocyte model was prepared using angiotensin Ⅱ（AngⅡ）, followed by interventions with URMC-099, Oligomycin A and YWHLR medicated serum. The mice were divided into control, AngⅡ, AngⅡ + URMC-099, AngⅡ + Oligomycin and AngⅡ + YWHLR groups. Cell viability and proliferation were assessed using the CCK-8 assay. RT-PCR measured the levels of MLK3 and UPR^mt mRNA（MLK3, CHOP, LONP1, HSP60, HSP70）, and Western blot analyzed the protein levels related to MLK3 and UPR^mt（MLK3, CHOP, LONP1, HSP60, HSP70）. Immunofluorescence double staining was employed to detect the expressions of HSP70 and MLK3 proteins.

Compared to the control group, the AngⅡ group showed a significant increase in MLK3 expression（P<0.01）and a significant decrease in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）. The AngⅡ + URMC-099 group exhibited a significant decrease in MLK3 expression（P<0.01）and a significant increase in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）compared to the AngⅡ group. The AngⅡ + Oligomycin group showed no significant difference in MLK3 expression（P>0.05）but a significant increase in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）. The AngⅡ + YWHLR group demonstrated a significant decrease in MLK3 expression（P<0.01）and a significant increase in the levels of CHOP, LONP1, HSP60 and HSP70（P<0.01）compared to the AngⅡ group.

YWHLR medicated serum may exert its therapeutic effects by modulating the MLK3-mediated mitochondrial unfolded protein response.

To analyze the quality of different forms of Dihuang（Radix et Rhizoma Dioscorea）（juice decoction, fresh dried decoction, decoction）, to determine the contents of ingredients and establish the fingerprint map of different forms of Dihuang（Radix et Rhizoma Dioscorea）, so as to provide a basis for the clinical application of different forms.

High Performance Liquid Chromatography（HPLC）measured the contents of three glycosides and five glycosides of Dihuang（Radix et Rhizoma Dioscorea）and established the HPLC fingerprint of different treatment forms.

The content order of three kinds of cyclic terpenoid components was fresh dried Dihuang（Radix et Rhizoma Dioscorea）decoction > Dihuang（Radix et Rhizoma Dioscorea） juice decoction > Dihuang（Radix et Rhizoma Dioscorea）decoction；the content order of 5 kinds of sugar was Dihuang（Radix et Rhizoma Dioscorea）juice decoction > fresh dried Dihuang（Radix et Rhizoma Dioscorea）decoction > Dihuang（Radix et Rhizoma Dioscorea）decoction. Twelve common peaks were calibrated in the fingerprint spectrum of different processing forms of Dihuang（Radix et Rhizoma Dioscorea）. Through the control comparison, six total peaks were identified, which were peach leaf coridin, reflavin D, reflavin A, motherthidin and hair flower glycoside. The similarity results showed that the similarity of different treatment forms of 30 batches ranged from 0.835 to 0.994, indicating a good similarity of the established fingerprint. Principal component analysis（PCA）and orthogonal least squares discriminant analysis（OPLS-DA）analysis showed differences in the quality of different treatment forms, and the main landmark components that differentiated different treatment forms were peaks 1, 2, 11, 12, 3（catalpol）, 10 and 5.

In this study, the contents of different forms of Dihuang（Radix et Rhizoma Dioscorea）（decoction, fresh dried decoction and decoction）were determined, and the fingerprint of different forms of Dihuang（Radix et Rhizoma Dioscorea）was established, which provided the research basis and theoretical basis for the primary processing of fresh Dihuang（Radix et Rhizoma Dioscorea）and the clinical use of the juice.

To observe the effect of clinical use of Lujiaojiao（Cervi Cornus Colla）in the treatment of patients with knee osteoarthritis of wind-cold-damp paralysis and deficiency of liver and kidney syndrome.

Ninety-four patients with knee osteoarthritis attending the Department of Orthopaedics of People＇s Hospital Affiliated to Shandong First Medical University from March 2024 to June 2024 were selected and divided into two groups by simple randomisation method. In the Isogia group, 47 cases（2 cases were dislodged and 45 cases were completed the trail）were treated with Glucosamine Sulfate Capsules（trade name: Isogia）, and in the Lujiaojiao（Cervi Cornus Colla）group, 47 cases（2 cases were dislodged and 45 cases were completed the trail）were treated with Lujiaojiao（Cervi Cornus Colla）combined with Isogia. The efficacy and safety of the two groups were counted, and the joint fluid matrix metalloproteinase-3（MMP-3）, metalloproteinase inhibitor-1（TIMP-1）, numerical rating scale（NRS）score, knee score, ultrasound parameters and serum bone metabolism level were compared between the two groups.

The total effective rate of the Lujiaojiao（Cervi Cornus Colla）group was 91.11%（41/45）. The total effective rate of the Isojia group was 75.56%（34/45）. The total effective rate of the Lujiaojiao（Cervi Cornus Colla）group was higher than that of the Isojia group, and there was a difference（P<0.05）. The comparison of knee joint score, ultrasound detection parameters, NRS score and score of main symptoms and signs of knee osteoarthritis before treatment between the two groups showed no difference（P>0.05）. The knee joint scores were higher in both groups after treatment and the scores were higher in the Deer Bone Glue group than those of the Isojia group（P<0.05）. Knee osteoarthritis major symptoms and signs score, synovial thickness, NRS score and joint fluid depth after treatment in both groups were lower than those before treatment, and the scores were lower in the Lujiaojiao（Cervi Cornus Colla）group than those of the Isojia group（P<0.05）. The comparison of joint fluid MMP-3, TIMP-1 and serum bone metabolism before treatment between the two groups showed no difference（P>0.05）. The levels of joint fluid TIMP-1 and serum osteocalcin（BGP）and bone-specific alkaline phosphatase（BALP）were elevated after treatment in both groups compared with those before treatment, and the levels were higher in the Deer Bone Glue group than those of the Isojia group（P<0.05）. The levels of joint fluid MMP-3, MMP-3/TIMP-1 and serum anti-tartrate acid phosphatase isoform 5b（TRACP-5b）and collagen type I C-terminal peptide（CTX-I）were lower in both groups after treatment than those before treatment, and the levels were lower in the Lujiaojiao（Cervi Cornus Colla）group than those of the Isojia group（P<0.05）. A total of 5 cases of adverse reactions occurred in the Lujiaojiao（Cervi Cornus Colla）group, with an incidence rate of 11.11%（5/45）；4 cases occurred in the Isojia group, with an incidence rate of 8.89%（4/45）. There was no difference in the cumulative incidence of adverse reactions between the two groups（P>0.05）.

Lujiaojiao（Cervi Cornus Colla）can maintain the dynamic balance of MMP-3/TIMP-1 in synovial fluid, improve bone metabolism and enhance the efficacy in the treatment for knee osteoarthritis.

To explore the effect of Cinobufagin Capsule combined with metronomic chemotherapy on tumor microenvironment and chemokine receptor 7（CXCR7）and CXCR4 in patients with non-small cell lung cancer（NSCLC）.

One hundred and twenty-two NSCLC patients admitted to the hospital from January 2022 to October 2023 were selected and randomly assigned into two groups using the random number table method, each with 61 cases. The control group was given 3 cycles of metronomic chemotherapy（pemetrexed + cisplatin）. Based on this, The study group was treated with Cinobufagin Capsule for 9 weeks. After treatment, the clinical efficacy, tumor markers[carcinoembryonic antigen（CEA）, cytokeratin 19 fragment（CYFRA21-1）, carbohydrate antigen 125（CA125）], immune function indicators（CD3⁺, CD4⁺, CD8⁺）, positive expression rates of CXCR7 and CXCR4 and adverse reactions were compared between two groups.

The object response rate and disease control rate were 40.98% and 62.30% in the control group, which were 67.21% and 86.89% in the study group, with statistical difference（P<0.05）. The serum levels of CEA, CYFRA21-1 and CA125 showed a reduction in both groups after treatment, and the three tumor markers were notably lower in the study group than those in the control group（P<0.05）. A decline in CD3⁺, CD4⁺ and CD4⁺/CD8⁺ and an increase in CD8⁺ were detected in both groups after treatment（P<0.05）, and the study group had higher levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ and lower CD8⁺ lower than those of the control group（P<0.05）. The positive rates of CXCR7 and CXCR4 showed a decrease in both groups after treatment, and were lower in the study group than those in the control group（P<0.05）. The overall incidence rate of adverse reactions including nausea, vomiting, neutropenia, thrombocytopenia and liver function damage in the study group was lower than that in the control group（P<0.05）.

The application of Cinobufagin Capsule combined with metronomic chemotherapy in NSCLC patients can effectively ameliorate the tumor microenvironment, and reduce the positive rates of CXCR7 and CXCR4, which is worthy of promotion.

This study aims to assess the efficacy of Laonian Gusong Formula（老年骨松方）with calcitriol in treating the elderly patients with osteoporotic intertrochanteric fracture of the femur.

A total of 160 elderly patients with osteoporotic intertrochanteric fractures of the femur were enrolled at the Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine from February 2022 to February 2024. The patients were randomly divided into a treatment group（80 cases） receiving Laonian Gusen Formula combined with calcitriol and a control group（80 cases）receiving calcitriol alone. The treatment lasted for 2 months. The therapeutic efficacy, pain relief[visual analogue scale（VAS）], bone mineral density（BMD）, bone metabolism markers[insulin-like growth factor-1（IGF-1）, growth hormone（GH）, bone gla protein（BGP）], daily living ability[Barthel index（BI）]and complication rates were monitored and evaluated.

The treatment group exhibited a significantly higher efficacy in traditional Chinese medicine syndrome treatment with a total effectiveness rate of 96.25%（77/80）, compared to 77.50%（62/80）in the control group（P<0.05）. Improvements in BI scores from 58.62 to 73.75 and reductions in VAS scores from 7.87 to 2.29 in the treatment group were significantly better than those in the control group（P<0.05）. Furthermore, the bone density significantly increased in the treatment group, with the density measurements at the greater trochanter and femoral neck rising from 0.19 to 0.43 and from 0.43 to 0.49, respectively（P<0.000）. Markers of bone metabolism such as IGF-1, GH and BGP also improved markedly（P<0.05）. Additionally, the incidence of complications including pressure ulcers, infections and nausea was significantly lower in the treatment group at 5.00%（4/80）compared to 27.50%（22/80）in the control group（P<0.05）.

The integration of Laonian Gusong Formula with calcitriol significantly enhances the bone density, alleviates the pain and promotes the functional recovery in the elderly patients with osteoporotic intertrochanteric fractures, proving to be an effective treatment strategy for these conditions.

To investigate the effect of Compound Danshen Dripping Pills（复方丹参滴丸）combined with edaravone and dexborneol concentrated solution on serum amyloid in the patients with acute cerebral infarction.

One hundred and fifty inpatients with acute cerebral infarction in the Neurology Department of Tangshan Gongren Hospital from January 2022 to January 2024 were selected and grouped according to different treatment methods. All participants were given butylphthalide treatment. Based on this, the control group（74 cases）was given edaravone and dexborneol concentrated solution, whereas the observation group（76 cases）added Compound Danshen Dripping Pills combined with edaravone and dexborneol concentrated solution. The treatment in both groups lasted for 10-14 d. Then the neurological function recovery, serum amyloid, glycated serum protein, retinol binding protein 4, hemorheological parameters, clinical efficacy and adverse reactions were compared between two groups.

The observation group reported a lower score on National Institutes of Health Stroke Scale and modified Rankin Scale, and a higher Barthel index compared to the control group（P<0.05）. The levels of serum amyloid, glycated serum protein and retinol binding protein 4 decreased in the observation group compared to those in the control group（P<0.05）. The high and low shear whole blood viscosities, plasma viscosity and hematocrit were all smaller in the observation group than those in the control group（P<0.05）. The observation group denoted a higher overall efficacy rate than the control group（P<0.05）, while no statistical difference was found in adverse reaction rate between two groups（P>0.05）.

The application of Compound Danshen Dripping Pills combined with edaravone and dexborneol concentrated solution in patients with acute cerebral infarction can effectively promote the recovery of neurological function, reduce the levels of serum amyloid, glycated serum protein and retinol binding protein 4, and ameliorate the blood viscosity.

To explore the curative effect of Xiaochaihu Jiangni Decoction（小柴胡降逆汤）assisted by esomeprazole in the treatment of gastroesophageal reflux disease（GERD）.

A total of 70 patients with GERD in the hospital from March 2020 to February 2022 were selected as the research objects, and they were randomly divided into two groups, with 35 cases in each group. Both groups were given the standard treatment plan of esomeprazole. After the treatment, the control group continued to intervene without drugs, and the observation group continued to be treated with Xiaochaihu Jiangni Decoction. The clinical efficacy, clinical symptom score before and after treatment, 24 h pH value of esophagus during the 6 months follow-up, and the serum levels of Leptin and interleukin-8（IL-8）of the two groups were statistically compared. And the recurrence during follow-up was compared.

The total effective rate of the observation group was 91.43%（32/35）higher than that of control group 65.71%（23/35）（P<0.05）. The scores of the clinical symptoms（heartburn, belching, acid reflux, retrosternal pain）of the observation group after treatment were lower than those of the control group, 24 h pH value of esophagus during the 6 months follow-up（24 h regurgitation times, regurgitation times >5 min, the longest regurgitation time and the total time percentage of pH <4）, the serum levels of Leptin and interleukin-8（IL-8）were lower than those of the control group（P<0.05）. There was no significant difference in the recurrence rate between the two groups（P>0.05）.

After esomeprazole treatment, the continuous maintenance treatment of Xiaochaihu Jiangni Decoction can effectively relieve symptoms, regulate the serum levels of Leptin and IL-8 and reduce 24 h pH value of esophagus of the patients with GERD, with significant effects and positive significance in preventing recurrence.

To investigate the clinical effect of Xinyue Capsule（心悦胶囊）combined with radial artery approach in the treatment of coronary artery disease.

In this study, 200 patients with coronary heart disease admitted from January 2022 to January 2024 were divided into two groups by random number table method, and were evenly divided into a single intervention group and a combined treatment group, with 100 cases in each group. The patients in the single intervention group received coronary intervention by radial artery approach. Those in the combined treatment group were treated with Xinyue Capsule combined with radial artery approach for coronary intervention. During the treatment process, 3 patients in the simple intervention group fell off（97 patients remained）, 4 patients in the combined treatment group fell off（96 patients remained）, and the data of patients in each group was compared, including the treatment effect, TCM syndrome effect, hemorheological indexes such as D-dimer and fibrinogen before and after treatment and left ventricular posterior wall thickness（LVPWT）, left ventricular ejection fractions（LVEF）, left ventricular end diastolic diameter（LVEF）, left ventricular end diastolic diameter, changes of cardiac function indexes such as left ventricular end diastolic diameter（LVEDD）, changes of serum N terminal proB type natriuretic peptide（NT-proBNP）, visualanalogue scale（VAS）and EuroQol five dimensions questionnaire（EQ-5D）.

The total effective rate of the combined treatment group was significantly higher than that of the simple intervention group（P<0.05）. After treatment, hemorheology indexes（D-dimer and fibrinogen, etc.）, cardiac function indexes（LVPWT, LVEF, LVEDD）, NT-proBNP level, VAS and EQ-5D scores of the patients in both groups were significantly improved. The theses indexes of the combined treatment group were all better than those of the simple intervention group（P<0.05）. The complication rate of the combined treatment group was lower than that of intervention group（P<0.05）.

The clinical effect of Xinyue Capsule combined with radial artery approach in the treatment of coronary artery intervention was relatively significant. After treatment, the patients＇ cardiac function was improved, the pain was relieved, the quality of life was improved, and the complications were reduced. The treatment is safe and reliable.

To study the clinical efficacy of Mahuang Lianqiao Chixiaodou Decoction（麻黄连翘赤小豆汤）combined with routine treatment of primary nephrotic syndrome（PNS）with damp-heat syndrome.

A total of 120 patients with PNS in the hospital from March 2022 to March 2024 were included and assigned into observation and control group randomly. The control group received conventional treatment. The observation group received Mahuang Lianqiao Chixiaodou Decoction additionally. The clinical efficacy, TCMsyndrome scores, the levels of urea nitrogen（BUN）, creatinine（Scr）, albumin（ALB）, 24 h urine protein, T lymphocyte CD4⁺, CD8⁺, CD4⁺/CD8⁺, triglycerides（TG）and total cholesterol（TC）and occurrence of adverse reactions were compared between the two groups after treatment.

The observation group detected a higher clinical efficacy rate compared to control group（P<0.05）. After treatment, the observation group had lower scores on TCM syndromes compared to control group（P<0.05）. The levels of BUN, Scr and 24 h urinary protein decreased, ALB increased and the improvement was more obvious in the observation group（P<0.05）. The levels of CD4⁺ and CD4⁺/CD8⁺ in both groups were increased than those before treatment, the levels of CD8⁺, TG and TC decreased, and the improvement was more obvious in the observation group（P<0.05）. No statistical difference was found in adverse reaction rate between two groups（P>0.05）.

Mahuang Lianqiao Chixiaodou Decoction has a high efficacy in treating PNS with damp-heat syndrome. It can improve the renal function, blood lipids and immune function of the patients and relieve the clinical symptoms while ensuring safety.

To investigate the effects and mechanisms of extracts from Baihuasheshecao（Hedyotis diffusa Willd）on the proliferation and apoptosis of renal cancer cells.

Thirty-two BALB/c mice inoculated with renal cancer Renca cells were randomly divided into model group, Baihuasheshecao（Hedyotis diffusa Willd）extract group, 5-fluorouracil（5-FU）group and transforming growth factor-β（TGF-β）/Smad pathway inhibitor（LY2157299）group, with 8 mice in each group. Corresponding doses were administered by gavage, and the model group was given an equal volume of physiological saline by gavage once a day for 14 days. The volume changes of transplanted tumor blocks in mice were recorded. The mice in each group were euthanized 24 hours after the last administration. The tumor mass was weighed, and the tumor inhibition rate was calculated. Western blot was used to detect the expressions of TGF-β1, p-Smad2, p-Smad3, Bcl-xl, Bad and cleaned-Caspase-3. Immunohistochemical method was used to detect the protein expressions of proliferating cell nuclear antigen Ki67 and PCNA. HE and TUNEL staining were performed on tumor tissues to detect the morphology and apoptosis of tumor cells.

Compared with the model group, the growth rate of transplanted tumors in all three experimental groups was inhibited, and the volume of transplanted tumors significantly decreased after 14 days of administration. Western blot results showed that compared with the model group, the three experimental groups up-regulated the expression levels of pro apoptotic proteins Bad and cleaned-Caspase-3, down-regulated the expressions of anti apoptotic protein Bcl-xl and pathway proteins TGF-β1, p-Smad2 and p-Smad3（P<0.05）. The immunohistochemical results showed that the expressions of Ki67 and PCNA in tumor tissues of the three experimental groups were significantly lower than those of the model group（P<0.05）. The results of HE staining and TUNEL staining showed that compared with those of the model group, the proliferation ability of renal cancer cells in the tumor tissues of the three experimental groups was inhibited, and the apoptosis rate was significantly increased（P<0.05）.

The extract of Baihuasheshecao（Hedyotis diffusa Willd）can inhibit the proliferation of renal cancer Renca cells and promote their apoptosis, which may be related to the inhibition of TGF-β/Smad signaling pathway activation.