To investigate the ethanol fractional precipitation of vinegar-baked Chaihu（Radix Bupleuri）polysaccharides（VBCP）and their effects on pharmacokinetics and tissue distribution of lamivudine.

The ethanol fractional precipitation was used to separate vinegar-baked Chaihu（Radix Bupleuri）polysaccharides.The phenol sulfuric acid method was used to determine the molecular weight，and the bicinchoninic acid（BCA）method was used to determine the protein content.The high performance gel permeation chromatography was used to determine the molecular weight，the high performance liquid chromatography was used to determine the monosaccharide composition，and the infrared spectrum was used to identify the characteristic groups of polysaccharide.Then，high-performance liquid chromatography was used to determine the concentration of lamivudine in plasma and tissue at different time points after oral administration of lamivudine and lamivudine+VBCPs.The pharmacokinetic parameters were calculated using DAS2.0 software，and the differences in pharmacokinetic parameters and tissue concentrations were compared using SPSS 20.0.

The sugar contents of VBCP-40，VBCP-60，and VBCP-80 were 65.53%，66.42%and 54.86%，respectively.The protein contents were 9.836%，6.214% and 8.744%，respectively.The molecular weight of three polysaccharides decreased with increasing alcohol concentration，and they were all composed of mannose，rhamnose，glucuronic acid，galacturonic acid，glucose，galactose and arabinose，but the proportions varied greatly.The infrared spectrum shows that all of them had typical polysaccharide characteristic peaks.The results of pharmacokinetic study showed that compared with the lamivudine group，the combination group of VBCP-60 and lamivudine significantly increased area under the concentration time curve（AUC_0-9，AUC_0-∞）and C_max，while decreased plasma clearance（CLz/F）.The results of tissue distribution study showed that VBCP-60 and VBCP-80 could increase the concentration of lamivudine in the liver；meanwhile，VBCP-80 could reduce its concentration in the kidneys.

Ethanol fractional precipitation can achieve the preliminary separation and purification of VBCP.VBCP-60 and VBCP-80 can increase the distribution of lamivudine in the liver，the anti-HBV effector organ of lamivudine，so they may be the active fractions for enhancing the efficacy of lamivudine.

To study the effects of Yifei JianpiRecipe（益肺健脾方）on aquaporin 3（AQP3），aquaporin 5（AQP5）and mucin 5AC（MUC5AC）in bronchial and lung tissues of chronic obstructive pulmonary disease（COPD）model ratswith lung and spleen deficiency syndrome.

Sprague-dawley rats were randomly segmented into blank group，model group，ambroxol group，dexamethasone group and Yifei Jianpi Recipe high，medium and low dose groups.The COPD ratmodel of lung and spleen deficiency was created by fumigation combined with lipopolysaccharide tracheal drip and Senna gavage and the pathologic morphology of bronchopulmonary tissues in rats was observed by hematoxylin-eosin（HE）staining and Alcian Blue-Periodic Acid Schiff（AB-PAS）staining，and the m RNA expressions of AQP3，AQP5 and MUC5AC in ratbronchopulmonary tissueswere detected by RT-qPCR，and protein expressions of AQP3，AQP5 and MUC5ACwere detected by Western Blot and immunohisto chemistry（IHC）methods，the mRNA expressions of AQP3，AQP5 and MUC5AC in rat bronchopulmonary tissues were detected by quantitative real-time polymerase chain reaction（RT-qPCR），and the protein expressions of AQP3，AQP5 and MUC5AC in rat bronchopulmonary tissues were detected by Western Blotand IHC methods.

Compared with those in the sham group，the bronchopulmonary tissues of rats in themodel group showed different degrees of damage，and HE staining demonstrated that there were tangible inflammatory cell infiltration in the airways of rats，and AB-PASstaining reviewed thata good deal of cupshaped cell hyperplasia could be seen in the bronchial tubes of rats.ThemRNA and protein expressions of AQP3 and AQP5 were significantly decreased and MUC5AC mRNA and protein expressions were clearly increased in the model group（P<0.01）.Compared with those in the modelgroup，the inflammatory pathology damage of the bronchopulmonary tissues and the proliferation of cup cells in the rats of each dose group showed different degrees of improvement，and the m RNA and protein expressions of AQP3 and AQP5 were increased to different degrees.Among them，the Yifei Jianpi Recipe high dose group，the aminoglossum group and the dexamethasone group showed significant and comparable levels of increase（P<0.05）；the MUC5AC mRNA and protein expressions were reduced to different degrees，among which the ambroxol group，the dexamethasone group and the Yifei Jianpi Recipe high dose group showed significant and comparable reductions（P<0.01）.

Yifei Jianpi Recipe can inhibit airway mucus hypersecretion and reduce airway inflammation in COPD model rats with lung and spleen deficiency syndrome，and its mechanism is related to up-regulation of AQP3 and AQP5 and down-regulation of MUC5AC transcription and expression.

To study the effectof Sanqi（Notoginseng Radix et Rhizoma）onmitochondrial energy metabolism of rats with adriamycin induced kidney fibrosis with blood stasis.

Thirty rats were divided into 6 groups random ly，including normal group，model group，Sanqi（Notoginseng Radix et Rhizoma）low-dose group，Sanqi（Notoginseng Radix et Rhizoma）medium-dose group，Sanqi（Notoginseng Radix et Rhizoma）high-dose group and positive control group，with 5 rats in each group.Themitochondrial structure of rat renal tissue was observed by electron microscopy.The contents of adenosine triphosphate（ATP），adenosine diphosphate（ADP），adenosine monophosphate（AMP），the activities of Na⁺ -K⁺ -ATPase，Ca²⁺ -Mg²⁺-ATPase and the activities ofmitochondrial respiratory chain complex enzymeⅠ-Ⅳwere detected by chemical method.The metabolites of urine were analyzed by liquid chromatograph and mass spectrometer.The differential metabolites were enriched by Kyoto Encyclopedia of Genes and Genomes（KEGG）metabolic pathway.

Under electron microscope，the mitochondrial structure of renal tissue in the model group was incomplete，and Sanqi（Notoginseng Radix et Rhizoma）could maintain the homeostasis of renalmitochondrial structure.Sanqi（Notoginseng Radix et Rhizoma）could increase the mitochondrial ATP contentof the renal tissue，the activities of Na⁺-K⁺-ATPase，Ca²⁺-Mg²⁺-ATPase andmitochondrial respiratory chain complex enzymeⅠ-Ⅳ.Sanqi（Notoginseng Radix et Rhizoma）could reduce the contents of mitochondrial ADP and AMP of renal tissue.After analysis and comparison among the groups，11 different urine metabolites were screened，including ferulic acid，forin，arabitol，acetamide，biotin，allantoic acid，6-methylmercaptopurine，4-hydroxy-2-quinolone，aminopentanedioate，6-methyladenine and moupinamide.Further enrichment of urinemetabolites by KEGG in different experimental groups showed that the aldosterone-regulated sodium reabsorption signal pathway was significantly different.

Sanqi（Notoginseng Radix et Rhizoma）regulates the differential urine metabolites and raldosterone-regulated sodium reabsorption signal pathway.Sanqi（Notoginseng Radix et Rhizoma）can improve mitochondrial energy metabolism and renal fibrosis in chronic kidney diseases（CKD）rats with blood stasis bymulti-target and multi-pathway.

To explore the effect of Buzhong Yiqi Decoction（补中益气汤）on abnormal autophagy of hippocampal neurons in autoimmune thyroiditis（AIT）modelmice based on themiR-190b/Pleckstrin homology domain leucine-rich repeat protein phosphatase（PHLPP1）/protein kinase B（AKT）/Forkhead BoxO3a（FOXO3a）pathway.

Fifty female NOD.H-2^h4 mice were randomly divided into blank control group（CG group），model group（MG group），low，medium and high dose groups of Buzhong Yiqi Decoction（BG-1，BG-2，BG-3 groups），with 10 mice in each group.The AITmodelwas established through pig thyroglobulin inductionmethod.After successfulmodeling，the BG-1，BG-2 and BG-3 groups were given 2.73，8.19 and 24.57 g/（kg·d）Buzhong Yiqi Decoction by gavage，respectively.The CG and MG groups were given an equal amount of distilled water by gavage，with a rest of1 day every 6 days for 8 consecutive weeks.Morriswatermaze experiment was used to test the spatial learning and memory abilities of mice.Enzyme linked immunosorbent assay（ELISA）was used to detect the levels of serum triiodothyronine（T₃），tetraiodothyronine（T₄），thyroid stimulating hormone（TSH）and thyroglobulin antibody（TgAb）in mice.Hematoxylin eosin（HE）stainingmethod was used to observe the morphological changes of mouse thyroid tissue.Transmission electron microscopy was used to observe the ultrastructure of hippocampal neurons in mice.Immunofluorescence staining was used to observe the positive expression of LC3 in the hippocampal CA1 region of mouse brain tissue.Reverse transcription polymerase chain reaction（RT-PCR）was used to detect the expressions ofmiR-190b-5p and PHLPP1 mRNA in mouse hippocampal tissue.The Wes fully automated Western blot quantitative analysis system detected the protein levels of Beclin1，LC3，P62，PHLPP1，AKT，p-AKT，FOXO3a and p-FOXO3a inmouse hippocampal tissue.The dual luciferase reporter gene experiment confirmed the targeting relationship bet weenmiR-190b-5p and PHLPP1.

Compared with those of the CG group，the escape latency of MG group was significantly prolonged（P<0.01），and the number of crossing platforms and target quadrant residence time were significantly reduced（P<0.01）.The serum TgAb content significantly increased（P<0.01）.The size of thyroid follicles varies，and there was a large amount of lymphocyte infiltration in the stroma of follicles and damaged hippocampal neuronal structure with the appearance of autophagosomes.The average fluorescence intensity of LC3 in hippocampal CA1 region increased（P<0.01）.The expression levels of Beclin1，LC3，PHLPP1 and FOXO3a proteins in hippocampal tissue increased，while the expression levels of P62，p-AKT/AKT and p-FOXO3a/FOXO3a proteins decreased（P<0.01）.ThemiR-190b-5p level in hippocampal tissue decreased，while the PHLPP1mRNA level increased（P<0.01）.Compared with the MG group，the BG-1，BG-2 and BG-3 groups significantly shortened the escape latency ofmice（P<0.05，P<0.01），and increased the number of times they crossed the platform and the target quadrant residence time（P<0.01）.The structure of thyroid follicular epithelial cells was relatively intact，and the infiltration of lymphocytes significantly improved.The morphology and structure of hippocampalneurons recovered，and autophagosomes decreased.The average fluorescence intensity of LC3 in hippocampal CA1 region decreased（P<0.01）.The expression levels of Beclin1，LC3，PHLPP1 and FOXO3a proteins in mouse hippocampal tissue decreased，while the expression levels of P62，p-AKT/AKT and p-FOXO3a/FOXO3a proteins increased（P<0.05，P<0.01）.ThemiR-190b-5p level in hippocampal tissue increased，while the PHLPP1 mRNA level significantly decreased（P<0.05，P<0.01）.The dual luciferase assay confirmed that PHLPP1 was a targeted regulatorymolecule of miR-190b-5p.

Buzhong Yiqi Decoction may improve abnormal autophagy in hippocampal neurons of AITmice by regulating themiR-190b/PHLPP1/AKT/FOXO3a signaling pathway，thereby reducing hippocampal neuronal damage and alleviating cognitive dysfunction.

Taking the odor as the research object，this paper revealed the scientific connotation of the qualitative standard that“the drugwith strong odor has good quality”of Tianma（Gastrodiae Rhizoma）.

The contents of9monomer components and polyphenols in 53 batches of Tianma（Gastrodiae Rhizoma）were determined by high performance liquid chromatography and Folin-Ciocalteu colorimetricmethod.The odor of the samples was quantified by electronic nose technology，and the correlation between the two was analyzed by bivariate correlation method.Taking the quantitative characteristic value of odor as the index，the qualitative identificationmodel of gas-based quality evaluation of differentgrades of Tianma（Gastrodiae Rhizoma）was established by using chemometrics method.

Gastrodin，protocatechuic acid，4-hydroxybenzaldehyde，vanillin，parishin B and polyphenols were all positively correlated with the odor.Differentgrades of Tianma（Gastrodiae Rhizoma）can be distinguished by PCA and DFA models.

The stronger the odor of Tianma（Gastrodiae Rhizoma）is，the higher the contents of the above-mentioned pharmacodynamic components are，which reveals the traditional mechanism that“the drug with strong odor has good quality”of Tianma（Gastrodiae Rhizoma）.The establishment of a qualitative identification model based on objective quantification of odor can be used for the identification of differentgrades of Tianma（Gastrodiae Rhizoma），which lays a foundation for the quality evaluation of Tianma（Gastrodiae Rhizoma）.

To explore the clinical effect of ultrasound-guided needle knife dissection combined with lumbar inclined plate method in the treatment of gluteal epithelial nerve compression syndrome based on the theory of“bone alignment and tendon flexibility”.

Sixty patients with gluteal epithelial nerve compression syndrome who received treatment in the Orthopedic Department of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from January 2023 to January 2024 were selected as the study subjects.Using the random number allocation method，the patients were randomly divided into a control group（30 cases）and an experimental group（30 cases）.The control group was treated with ultrasound-guided needle knife dissection，while the experimental group was treated with a combination of ultrasound-guided needle knife dissection and lumbar inclined plate dissection under the guidance of the“bone alignment and tendon flexibility”theory.The treatment period for both groupswas 4 weeks.After treatment，the clinical efficacy，Visual Analog Scale（VAS）score，Oswestry Disability Index（ODI）score，shear wave Young'smodulus values of the lumbar multifidus and psoasmajor muscles，and the changes in surface electromyography were compared between the two groups.

Compared with the control group，the experimental group had a higher clinical total effective rate after 4 weeks of treatment，but there was no statistically significant difference in the total effective rate between the two groups（P>0.05）.Compared with those before treatment，VAS scores and ODI scores in both groups showed a decreasing trend after treatment and 4 weeks follow-up（P<0.05）.Compared with those of the control group，the VAS score and ODIscore of the experimental group were lower after treatment and 4 weeks follow-up（P<0.05）.Compared with those before treatment，the shearwave Young'smodulus values of the bilateral multifidus and lumbar muscles in the experimental group showed a decreasing trend after treatment and 4 weeks follow-up（P<0.05）.Compared with those before treatment，there was no statistically significant difference in the shearwave Young'smodulus values of the bilateral multifidus and lumbar muscles in the control group after treatment and 4 weeks follow-up（P>0.05）.Compared with the control group，the shearwave Young'smodulus values of the bilateral multifidus and lumbar muscles in the experimental group were lower after treatment and 4 weeks follow-up（P<0.05）.Compared with those before treatment，the average amplitude of electromyography on both sides of the multifidus and lumbar muscles in the experimental group showed an increasing trend after treatmentand 4 weeks follow-up（P<0.05）.Compared with those before treatment，there was no statistically significant difference in the average amplitude of surface electromyography of the multifidus and psoasmajormuscles in the control group after treatmentand 4 weeks follow-up（P>0.05）.Compared with those in the control group，the average amplitude of surface electromyography of the multifidus and psoasmajor muscles in the experimental group was higher after treatment and 4 weeks follow-up（P<0.05）.

The combination of ultrasound-guided needle knife dissection method and lumbar inclined plate method based on the concept of“bone alignment and tendon flexibility”can significantly improve the short-term and long-term clinical effects of treating gluteal epithelial nerve compression syndrome，with advantages such as high safety，minimal trauma and rapid recovery.

To investigate the effects of preparation and processing methods on the contents of oil components in Dihuang（Rehmanniae Radix）and clarify the correlation between oil components with medicinal properties，in order to provide guidance for processing research and clinical application.

The extraction of volatile oil refers to the volatile oil determination method A in the 2020 edition of Chinese Pharmacopoeia，and the Soxhlet method was used to extract fatty oil.Gas Chromatography Mass Spectrometry（GC-MS）was used to identify the oil components and their relative contents.Thermographic analysis was used to clarify the characteristics of the influence of different preparation and processing methods on oil components.Combined with literature review，the correlation of processing，preparation，components and properties was discussed.

The oil components weremainly fatty acids.The order of the extraction rate of fatty oil was fresh Dihuang（Rehmanniae Radix）>raw Dihuang（Rehmanniae Radix）>steaming with wine≈stewing with wine>steaming and exposing nine times with Sharen（Amomi Fructus）>steaming without ingredients，and the order of the volatile oil extraction rate was stewing with wine>steaming with wine>steaming and exposing nine times with Sharen（Amomi Fructus）>raw Dihuang（Rehmanniae Radix）≈steaming without ingredients>fresh Dihuang（Rehmanniae Radix）.The heatmap analysis of fatty oil and volatile oil showed that Shudihuang（Rehmanniae Radix Praeparata）stewing with wine and that steaming and exposing nine times with Sharen（Amomi Fructus）were clustered one category.

The changes and adjustments of the types and relative contents of oil componentswere important material basis for the differences in medicinal property of Dihuang（Rehmanniae Radix）processed products.Adding wine，Sharen（Amomi Fructus）or increasing steaming and exposing times were helpful in reducing the cold nature of Dihuang（Rehmanniae Radix），and the greasy nature of Shudihuang（Rehmanniae Radix Praeparata），and enhance thewarming-reinforcing effect.The volatile oil components in different products of Dihuang（Rehmanniae Radix）were the material basis of their“Qiwei”.

To observe the effect of Yinzhihuang Granules（茵栀黄颗粒）combined with ursodeoxycholic acid on the efficacy and liver function of patients with chronic jaundice hepatitis B.

A totalof132 patientswith chronic jaundice hepatitis B were enrolled in the hospital and random ly divided into observation group and control group according to the random number table method，with 66 cases in each group.The control group was treated with ursodeoxycholic acid，and the observation group was treated with Yinzhihuang Granules combined with ursodeoxycholic acid.The clinical efficacy，liver function，traditional Chinese medicine syndrome score，hepatitis B virus deoxyribonucleic acid（HBV DNA）negative conversion rate and occurrence of adverse reactions were observed in the two groups before and after treatment.

After treatment，the total effective rate of the observation group was 89.39%，which was significantly higher than that of the control group（74.24%）（P<0.05）.The liver functions of the two groups were alanine aminotransferase（ALT），aspartate aminotransferase（AST），total bilirubin（TBIL），total bile acids（TBA）andγ-glutamyl transferase（GGT）were all lower than those before treatment（P<0.05），while observing the liver function indicators of the observation group were significantly lower than those of the control group（P<0.05）.The TCM syndrome scores of the two groups were significantly lower than those before treatment（P<0.05）.At the same time，the TCM syndrome scores of the observation group were significantly lower than those of the controlgroup（P<0.05）.The HBV DNA negative conversion rate in the observation group was 56.06%，and that in the control group was 28.79%.The negative conversion rate in the observation group was significantly higher than that in the control group（P<0.05）.The incidence of liver injury in the observation group was 1.52%，which was significantly lower than that（13.64%）in the control group（P<0.05）.The incidence of adverse reactions in the observation group was 3.03%，which was not significant compared with that（10.61%）in the control group（P>0.05）.

Yinzhihuang Granules combined with ursodeoxycholic acid has a significant effect on patientswith chronic jaundice hepatitis B.It can effectively improve the clinical symptoms and liver function of patients，promote the HBV DNA negative conversion rate，and reduce the incidence of adverse reactions.

To study the clinical study of Jingu Tongxiao Pill（筋骨痛消丸）combined with arthroscopy in the treatment of knee osteoarthritis（cold coagulation blood stasis syndrome）.

A total of 120 patients diagnosed as knee osteoarthritis（cold coagulation and blood stasis syndrome）were selected and divided into two groups according to random number table.The reference group（60 cases）received arthroscopic treatment，and the study group（60 cases）received Jingu Tongxiao Pill and arthroscopic treatment.The clinical efficacy，TCM syndrome score，osteoarthritis index，inflammatory factor level and oxidative stress reaction of the two groupswere observed and compared.

Compared with that（75.00%）of the reference group，the total effective rate of the study group was98.33%，and the difference was statistically significant（P<0.05）.After treatment，the TCM syndrome score of the study group was lower than that of the reference group，and the difference was statistically significant（P<0.05）.After treatment，compared with those of the reference group，the pain，stiffness and difficulty scores of daily activities in the study group were lower，and the difference was statistically significant（P<0.05）.After treatment，compared with those of the reference group，the levels of tumor necrosis factor-alpha（TNF-α），interleukin-1 beta（IL-1β）and matrixmetalloproteinase-3（MMP-3）in the study group were lower，and the difference was statistically significant（P<0.05）.

The patients with knee osteoarthritis（cold coagulation blood stasis syndrome）were treated with arthroscopy and Jingu Tongxiao Pill，and the therapeutic effect was good，which could effectively improve the clinical symptoms and promote the recovery of patients，and the clinical application value was high.

To observe the clinical efficacy of Wangbi Capsule（尪痹胶囊）in the treatment of dampness-heat blocking collateral type of rheumatoid arthritis and its effects on serum granulocyte-macrophage colony-stimulating factor（GM-CSF），tumor necrosis factor-α（TNF-α）and CD4⁺T cell subsets.

Ninety-eighty patients with rheumatoid arthritis treated in the Rheumatology and Immunology Department of the hospital from June 2020 to June 2023 were selected as the study objects，and the above patients were divided into control group and observation group with 49 cases in each group by random number table method.The control group was given leflunomide orally，and the observation group was given Wangbi Capsule on the basis of the control group.The clinical efficacy，TCM syndrome score and adverse reactions，the degree of joint pain and the levels of serum inflammatory factors before and after treatment were compared between two groups.

The effective rate of the control group was 79.59%，and that of the observation group was 93.88%.Compared with that of the control group，the effective rate of the observation group was significantly increased（P<0.05）.There was no significant difference in TCM syndrome scores between the two groups before treatment（P>0.05）.After treatment，the levels of morning stiffness，joint flexion and extension，fever，joint swelling pain and heat in the observation group were significantly decreased compared with those in the control group（P<0.01）.Before intervention，there was no significant difference in ultrasound scores between the two groups（P>0.05）.After the intervention，the ultrasound scores in the observation group were significantly higher than those in the control group（P<0.05）.Before intervention，there was no significant difference in the degree of joint pain between the two groups（P>0.05）.Compared with those of the control group，visual analog scale（VAS）score and disease activity score 28（DAS28）score of the observation group were significantly decreased after treatment（P<0.01）.There was no significant difference in serum inflammatory factors between the two groups before intervention（P>0.05）.After the intervention，the levels of erythrocyte sedimentation rate（ESR），C-reactive protein（CRP），rheumatoid factor（RF），GM-CSF and TNF-αin the observation group were significantly decreased compared with those in the control group（P<0.01）.During the intervention，the main adverse reactions of all patients were gastrointestinal discomfort，hemocytopenia，elevated aminotransferase and skin allergy，and there was no significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Wangbi Capsule combined with leflunomide has a good clinical effect in the treatment of moisture-heat blocking collateral type of rheumatoid arthritis，which can alleviate the joint pain and dysfunction，improve the jointultrasound status and inhibit the inflammation，without increasing the adverse reactions.

To investigate the effectiveness of acupuncture at scalp acupoint penetration acupuncture combined with warming needle moxibustion in improving cognitive function and quality of life in patients with vascular dementia（VD）.

A total of124 patients with Qistagnation and blood stasis type of VD admitted to the hospital between August2017 and July 2019 were selected and divided into the needle therapy group（scalp acupoint penetration acupuncture combined with warming needle moxibustion）and the drug group（butylphthalide soft capsule），62 each in each.Both groups were treated for a total of6 weeks.The clinicalefficacy，traditional Chinese medicine syndrome scores，mini-mental state examination（MMSE），Hasegawa dementia status scale（HDS-R），functional activity questionnaire for social functioning（FAQ），activities of daily living（ADL）and adverse effects occurrence results were observed and recorded for comparison.

The total efficiency of the needle therapy group（82.26%）was significantly higher than thatof the drug group（61.29%）（P<0.05）.Before treatment，the differences in traditional Chinese medicine syndrome scores，the scores of MMSE，HDS-R，FAQ and ADL between the needle therapy group and the drug group were notstatistically significant（P>0.05）.After treatment，the traditional Chinese medicine syndrome scores and FAQ scores were significantly lower（P<0.05），and the scores of MMSE，HDS-R and ADL were significantly higher（P<0.05），and the traditional Chinese medicine syndrome scores and FAQ scores in the needle therapy group were significantly lower than those in the drug group（P<0.05），and the scores of MMSE，HDS-R and ADL in the needle therapy group were significantly greater than those in the drug group（P<0.05）.The differences in the incidence of the total adverse reactions between the needle therapy group and the drug group were not statistically significant（P>0.05）.

The efficacy of scalp acupoint penetration acupuncture combined with warming needle moxibustion in treating VD can effectively improve the patients'cognitive function and quality of life，with good safety，which is worthy of clinical promotion and application.

To observe the clinical efficacy of Qingre Jiedu Decoction（清热解毒汤）in the treatment of severe psoriasis vulgaris（blood-heat type）and its impacton serum inflammation and immune function in patients.

A prospective study was conducted on 100 patients with psoriasis vulgaris who received treatment at Shengli Oilfield Central Hospital in Dongying City.They were divided into a control group and an observation group in the order of admission，with 50 patients in each group.The control group received conventional Western medicine treatment，while the observation group received oral administration with Qingre Jiedu Decoction for 8 weeks.The total effective rate，traditional Chinese medicine syndrome scores，serum inflammation level，immune function and adverse reactions between two groups were compared.

The total effective rate of the observation group（96.00%）was significantly better than that（84.00%）of the control group，and the difference was statistically significant（P<0.05）.Before intervention，there was no significant difference in skin lesions and quality of life scores between the two groups（P>0.05）.After8 weeks of intervention，compared with those of the controlgroup，the psoriasis area and severity index（PASI）and dermatology life quality index（DLQI）of the observation group were significantly reduced（P<0.01）.The scores of symptoms such as rash color，burning sensation，itching and irritability in the observation group significantly decreased（P<0.01）.There was no significant difference in serum inflammation or T lymphocyte subsets levels between the two groups before intervention.After 8 weeks of intervention，the levels of serum tumor necrosis factor-α（TNF-α），transforming growth factor-β（TGF-β）and interleukin-6（IL-6）of the observation group were significantly down-regulated compared to those of the control group（P<0.01）.The levels of CD3⁺，CD4⁺and CD4⁺/CD8⁺in the observation group were significantly increased compared to those in the control group，while the levels of CD8⁺were significantly decreased，with statistical significance（P<0.01，P<0.05）.The incidence of adverse reactions in the observation group（16.00%）showed no significant difference compared to that（10.00%）in the control group（P>0.05）.

The combination of Qingre Jiedu Decoction（Psoriasis External Wash No.1 Formula）and Avia Capsules can improve the clinical efficacy in patients with severe psoriasis vulgaris，further improve the clinical symptoms，enhance the quality of life and significantly reduce PASI，and the mechanism of action is related to inhibiting the body's inflammatory response and regulating immune function.

To explore the effect of Qili Qiangxin Capsule（芪苈强心胶囊）on chronic heart failure and its influence on the levels of troponin（cTNI），homocysteine（Hcy），N-terminal B-type natriuretic peptide（NT-proBNP）and highsensitivity C-reactive protein（hs-CRP）in patients.

Ninety-two patients with chronic heart failure were selected and divided into control group and comprehensive group，46 cases in each group.The conventional comprehensive treatment was given，and the comprehensive group was treated with Qili Qiangxin Capsule for 3 months.The therapeutic effect，TCM syndrome scores，cardiac function and the changes of serum levels of cTNI，Hcy，NT-proBNP and hs-CRPwere observed.

There was no significant difference between the two groups in TCM syndrome scores before treatment（P>0.05），but the score decreased after treatment（P<0.05），and the score of the comprehensive group was lower than that of the control group（P<0.05）.There was no significant difference of stroke volume（SV），left ventricular end diastolic diameter（LVEDD），left ventricular ejection fraction（LVEF）or 6-minute walking test distance（6MWT）between the two groups before treatment（P>0.05），but the values of SV，LVEF and 6MWT increased after treatment（P<0.05），and these indexes of the comprehensive group were higher than those of the control group（P<0.05）.The levels of serum cTNI，Hcy，NT-proBNP and hs-CRP in the two groups were not statistically different before treatment（P>0.05），but decreased after treatment（P<0.05），and the levels of the comprehensive group were lower than those of the control group（P<0.05）.The overall curative effect of the comprehensive group was better than that of the control group（P<0.05）.

The treatment of chronic heart failure with QiliQiangxin Capsule is helpful to reduce the serum levels of cTNI，Hcy，NT-proBNP and hs-CRP，improve the symptoms and cardiac function of patients，and then improve the therapeutic effect.

To observe the clinical effect of Viral Flu Prescription combined with external treatment by pressing needle in the treatment of acute viral upper respiratory tract infection.

This study included 200 patients with acute viral upper respiratory tract infection（admitted to the emergency department and fever clinic from October 2023 to March 2024），who were divided into observation group and control group by random number tablemethod.The control group（100 cases）received conventional treatment.The observation group（100 cases）were treated with Viral Flu Prescription and external treatment by pressing needle on the basis of the control group.The data of patients in each group was compared，including the treatmenteffect，the time of body temperature returned to be normal，the time of cough，sore throat and runny nose to disappear，adverse reactions and TCM symptom scores before and after treatment（fever，sore throat，drymouth，thirst，stream turbidity stuff，etc.）and the levels of serum amyloid A（SAA），tumor necrosis factor-α（TNF-α），interleukin-6（IL-6），interleukin-10（IL-10）and other inflammatory factors.

The total effective rate of the observation group（99.00%，99/100）was higher than that of the control group（93.00%，93/100）（P<0.05）.Compared with those in the control group，the time of body temperature to return to be normal，the time for cough，sore throat and runny nose to disappear were shorter in the observation group（P<0.05）.The adverse reaction rate of the control group and the observation group was lower（P>0.05）.Before treatment，TCM symptom scores（fever，sore throat，dry and thirsty mouth，runny nose，etc.），the levels of SAA levels，TNF-α，IL-6 and IL-10 were compared in both groups（P>0.05）.After treatment，these indexes in the observation group were better than those in the control group（P<0.05）.

Viral Flu Prescription combined with external treatment by pressing needle has good clinical effect in the treatment of acute viralupper respiratory tract infection，which can better improve the symptoms of patients，promote patients to recover faster，and the treatment is safe and reliable.

To explore the mechanism of Modified Qingre Shenshi Decoction（清热渗湿汤化裁）regulating TLR4/NF-κB signaling pathway in improving ulcerative colitis.

A total of150 patients with ulcerative colitis admitted to the hospital from January 2021 to January 2024 were selected and divided into control group（75 cases，treated with mesalazine enteric-coated tablets）and observation group（75 cases，treated with Modified Qingre Shenshi Decoction）according to a random number table.The efficacy and adverse reactions of the two groupswere compared.TCM syndrome scores，disease evaluation，serum factor and mRNA expressions of TLR4 and NF-κB in two groups were observed.

After treatment，the total effective rate of the observation group was higher than that of the control group（P<0.05）.Before treatment，the scores of lower abdomen fullness and pain，burning anus，strong anal distension，diarrhea，mucus，pus and blood stool，heavy limbs，chest tightness and fever，dry mouth and bitter mouth and short urine red were not significant between the two groups（P>0.05）.After treatment，the scores of the above symptoms were all lower than those before treatment.In addition，the scores in the observation group were significantly lower than those in the control group（P<0.05）.Before treatment，the scores of Baron endoscopy and mucosal histology were not significant between the two groups（P>0.05）.After treatment，the scores of Baron endoscopy and mucosal histology in the two groups were lower and the scores in the observation group were significantly lower than those in the control group（P<0.05）.Before treatment，the levels of interleukin-1β，tumor necrosis factor-α，endotoxin and D-lactic acid were not significant between the two groups（P>0.05）.After treatment，the levels of interleukin-1β，tumor necrosis factor-α，endotoxin and D-lactic acid in both groups were lower than those before treatment，and the levels in the observation group were significantly lower than those in the control group（P<0.05）.Before treatment，the mRNA expressions of TLR4 and NF-κB not significant between the two groups（P>0.05）.After treatment，the mRNA expressions of TLR4 and NF-κB in the two groups were lower than those before treatment，and the expressions in the observation group were significantly lower than those in the control group（P<0.05）.There were no adverse reactions in both groups during the treatment.

By regulating TLR4/NF-κB signaling pathway，Modified Qingre Shenshi Decoction can improve the clinical symptoms of patients with ulcerative colitis，plays an anti-inflammatory role and promotes the recovery of patients，which is worthy of clinical application.

To establish amethod for identifying Shicangpu（Acori Tatarinowii Rhizoma）（ATR）from common counterfeits by ultra performance liquid chromatography（UPLC）technology，and to provide reference for quality evaluation and control of ATR.

Waters CORTECST3 column（2.1 mm×150 mm，1.6μm）was used.Methanol：acetonitrile 1：1（A）-water（B）solution was used as mobile phase and gradientelution was performed.The flow ratewas0.2mL/min.The column temperature was40℃.The detection wavelength was275 nm and the sample volume was3μL.The UPLC characteristic map was established to identify ATR from common counterfeits.

The UPLC characteristic map identified 4 common peaks of ATR and identified 1 of them.The similarity among samples was 0.892～0.996.

The established method of characteristic map is simple，reliable，accurate and repeatable，which can identify the authenticity of ATR directly，provide a specific method for the quality control and authenticity identification of ATR，and effectively improve the drug safety of related products.

To investigate the effects of vinegar-procssed Chaihu（Radix Bupleuri）and its differentextraction parts on the uptake of cisplatin（DDP）by cells with high expression of organic cation transporter 2（OCT）（hOCT2-HEK293）and cells with high expression ofmultidrug resistance associated protein 2（MRP2）（hMRP2-HEK293）and to preliminarily explore their mechanisms so as to provide data support for the exploration of the mechanism of using vinegar-procssed Chaihu（Radix Bupleuri）to assist in reversing DDPmultidrug resistance in tumor cells in clinical practice.

The hOCT2-HEK293 and hMRP2-HEK293 cells were co-incubated with vinegar-procssed Chaihu（Radix Bupleuri）and its different parts for 24 hours，and then DDP was added to continue incubation for 4 hours.The intracellular DDP content of the two types of cells was measured by HPLC.Western Blot and real-time quantitative PCR（RT-PCR）were used tomeasure the protein and gene expressions of OCT2 and MRP2 in two types of cells，respectively.

In hOCT2-HEK293 cells，the total extract，polysaccharide fraction and small molecule water-soluble fraction of vinegar-procssed Chaihu（Radix Bupleuri）can significantly promote the uptake of DDP，and all promote the protein and gene expressions of OCT2.In hMRP2-HEK293 cells，the total extract，polysaccharide fraction and small molecule water-soluble fraction of vinegar-procssed Chaihu（Radix Bupleuri）also significantly promoted the uptake of DDP，but only the total extract could significantly reduce the expression of MRP2 protein.

In tumor cells，the reversal of multidrug resistance to anti-tumor drugs by vinegar-procssed Chaihu（Radix Bupleuri）may be related to the up-regulation of OCT2 protein and mRNA expressions in polysaccharide and small molecule water-soluble sites，or the down-regulation ofMRP2 protein expression in vinegar-procssed Chaihu（Radix Bupleuri）total extract.Preliminary speculation suggests that affecting the functions of OCT2 and MRP2 is one of the mechanisms by which vinegar-procssed Chaihu（Radix Bupleuri）enhances the meridian stimulation.

To study the effect of Wendantang Huacaifang（WDTHCF）on atrial energy metabolic remodeling in paroxysmal atrial fibrillation（PAF）rats based on fatty acid metabolic pathway.

Male SD rats were selected and randomly divided into the normal group，the model group，the WDTHCF low，medium and high dose groups，and the amiodarone hydrochloride group，and the PAF model was induced by using Ach-CaCl_2.After the model was successfully established，the WDTHCF low，medium and high dose groups and amiodarone hydrochloride were given by gavage.The duration of atrial fibrillation was monitored using electrocardiography.The formation of atrial lipid droplets was detected by oil-red O staining.The contents of free fatty acid（FFA）and triglyceride（TG）of atrial tissues were detected.RT-qPCR was used to detect the mRNA expressions of aAMP-activated protein kinase（AMPK），acetyl coenzyme A carboxylase（ACC），carnitine palmitoyltransferase-1B（CPT-1B），fatty acid translocase/cluster of differentiation 36（FAT/CD36），fatty acid transport protein（FATP）and fatty acid binding protein 3（FABP3）.Western blot was used to detect the protein expressions of AMPK，p-AMPK，ACC，p-ACC and CPT-1B and immunohistochemistry method was used to detect myocardial translocation of atrial FAT/CD36.

Compared with the normal group，the rats in themodel group showed increased duration of AF（P<0.05），elevated contents of FFA and TG in the atria（P<0.05），significant lipid droplet formation in the atria，decreased mRNA expressions of AMPK，ACC，CPT-1B，FAT/CD36，FATP and FABP3（P<0.05），and decreased atrial tissue AMPK phosphorylation，ACC phosphorylation and relative protein expression of CPT-1B were significantly reduced（P<0.05），and the membrane translocation of FAT/CD36 in the atria was reduced（P<0.05）.After treatment with WDTHCF，PAF duration was shortened（P<0.05），and the contents of FFA and TG in the atria decreased（P<0.05），lipid droplet formation in atria decreased，the mRNA expressions of AMPK，ACC，CPT-1B，FAT/CD36，FATP and FABP3 in atrial tissues were elevated（P<0.05），and the relative protein expressions of AMPK phosphorylation，ACC phosphorylation and CPT-1B in atrial tissues were elevated（P<0.05），and the membrane translocation of FAT/CD36 in atria was increased.

WDTHCF improved atrial energy metabolic remodeling in phlegm-turbid and bloodstasis type of PAF rats through AMPK regulating the ACC/CPT-1Bmediated fatty acid metabolism pathway.

To investigate the impact of Schisandrol A（SA）on lipopolysaccharide（LPS）-induced and differentiated small intestinal epithelial cell damage in human colorectal cancer Caco-2 cells.

Caco-2 cells were random ly divided into a normal control group（without drug intervention，Con），a model group（0.7 mg·mL^-1，LPS）and SA（2.5，5，10 μmol·L^-1）dose groups.The cell viability was assessed by the CCK-8 method.The cell apoptosis，cell cycle，mitochondrial membrane potential and intracellular reactive oxygen species（ROS）were detected by flow cytometry.The levels of inflammatory factors in the cell supernatant were measured using ELISA.Oxidative stress was assessed using an enzyme-labeled method.The expressions of proteins related to the NF-κB signaling pathway and apoptosis-related pathways were determined by Western Blot analysis.

The findings indicated that compared with the LPS group，SA at concentrations between 2.5-10μmol·L^-1 exhibited a significant protective effect on cells，exhibiting a certain dose-dependent relationship（P<0.01，P<0.001）.It reduced the contents of malondialdehyde（MDA），lactate dehydrogenase（LDH）and ROS（P<0.05，P<0.01，P<0.001），increased the activities of superoxide dismutase（SOD）and GSH（P<0.01，P<0.001），inhibited the secretion of pro-inflammatory factors tumor necrosis factor-α（TNF-α），interleukin-6IL-6 and interleukin-18（IL-18）（P<0.05，P<0.01，P<0.001），promoted the release of anti-inflammatory factor interleukin-10（IL-10）（P<0.001），increased mitochondrial membrane potential（P<0.05，P<0.01），improved cell cycle arrest（P<0.05）and modulated the expression levels of inflammation and apoptosis-related pathway proteins（P<0.05，P<0.01，P<0.001）.

This study has confirmed in vitro that SA is capable of improving LPS-induced damage to Caco-2 cells.The mechanism of action appears to be related to the inhibition of the NF-κB signaling pathway.

To analyze the study of the utility of five-element identification acupuncture in traditional Chinese medicine combined with ABA training in the treatment of children with autism.

Ninety cases of autistic children admitted to the Department of Pediatrics of Nanyang First People's Hospital Affiliated to Henan University from March 2022 to March 2024 were selected for the study.Forty-five cases of the control group were treated with ABA training，and the other 45 cases of the observation group were treated with traditional Chinese medicine five-element identification acupuncture on the basis of the above mentioned treatment，and the clinical effects，the scores on the scales［（Autism Treatment and Evaluation Scale（ATEC），Children's Autism Rating Scale（CARS），Autism Behavioral Scale（ABC）］，serological indexes［tissue-type plasminogen activator（t-PA）and plasminogen activator inhibitor-1（PAI-1）］of the two groups were compared.

The total effective rate of the observation group（97.78%，44/45）was higher than that（82.22%，37/45）of the control group and the difference was significant（P<0.05）.The ATEC scores of both groups were reduced after one and three months of treatment（P<0.05），and the reduction wasmore significant in the observation group compared with that in the control group（P<0.05）.CARSscore and ABC score were reduced in both groups after one and three months of treatment（P<0.05），and the reduction was more significant in the observation group compared with that in the controlgroup（P<0.05）.After one and three months of treatment，t-PA increased and PAI-1 decreased in both groups（P<0.05），and compared with those in the control group，t-PA increased more significantly and PAI-1 decreased more significantly in the observation group（P<0.05）.

After the children diagnosed with autism were treated with the combination of ABA training and traditional Chinese medicine five-element identification acupuncture，the therapeutic effect was remarkable.The treatment could effectively reduce the children's clinical symptoms，improve their serological indexes and promote the recovery of the children.

The differential metabolites of Sangshen（Mori Fructus）with different maturities were studied to provide reference for the determination of Sangshen（Mori Fructus）harvest time.

Based on the metabolo micsmethod，the changes of chemical components in Sangshen（Mori Fructus）with different maturities were explored.The data of secondary metabolites in mulberry fruits was collected by ultra-high performance liquid chromatography-quadrupole/exactive Orbitrap mass spectrometry（UPLC-Q-Exactive Orbitrap MS）.Multivariate statistical analysis methods such as principal component analysis（PCA）and partial least squares discriminant analysis（PLS-DA）were used to explore the differences in metabolic components of Sangshen（Mori Fructus）with different maturities.

There were significant differences in the metabolites of Sangshen（Mori Fructus）with different maturities.A total of176 compounds in 11 categories were screened.Flavonoids and organic acids accounted for 23.30% and 21.02%，respectively.The contents of most screened flavonoids and organic acid differential metabolites increased with the increase of maturity，and the flavonoids and organic acid metabolites of Sangshen（Mori Fructus）of different germplasms were different.The contents of extract，total flavonoids，total polyphenols and rutin in the mature stage were higher than those in the immature stage.The antioxidant activity in the mature stage was stronger than that in the immature stage.The content of chlorogenic acid in the immature stage was higher than that in the mature stage，and the inhibitory effectonα-glucosidase was also stronger than that in the mature stage.

There are obvious differences in the metabolites of Sangshen（Mori Fructus）with different maturities.The contents of chemical components in Sangshen（Mori Fructus）are related to different maturities.Based on the antioxidant effect，it is better to harvest when Sangshen（Mori Fructus）is dark purple（mature）.Based on the hypoglycemic effect，it is better to harvest when the Sangshen（Mori Fructus）becomes red（immature），which provides a basis for the quality control of Sangshen（Mori Fructus）and the determination of harvesting time.

This study aimed to explore the clinical efficacy of Huanglian Wendan Decoction（黄连温胆汤）combined with acupoint application in the treatment of patients with dampness-heat of spleen and stomach type of functional dyspepsia（FD）and its impacton patients'quality of life and psychological well-being.

This study selected patients diagnosed as dampness-heat of spleen and stomach type of functional dyspepsia（FD）and treated at the hospital from October2021 to October 2023 as the research subjects，totaling 116 cases.According to the random number table method，they were divided into a control group（53 cases）and an experimental group（53 cases）.The patients in the control group received oral treatment with Mosapride Citrate Tablets，while those in the experimental group were treated with Huanglian Wendan Decoction combined with acupoint application on the basis of Mosapride Citrate Tablets.The efficacy of treatments and traditional Chinese medicine（TCM）syndrome scores were assessed and compared between the two groups.The levels of serum motilin（MTL），gastrin（GAS）and ghrelin before and after treatment were statistically compared.The Beck Depression Inventory（BDI），Beck Anxiety Inventory（BAI），Nipine Digestive Dysplasia Life Index Scale（NDLQL）and Medical Outcomes Study Health Survey Short Form-36 Item（SF-36）were used to evaluate the patients'quality of life and psychological state.Adverse reactions to the treatments in both groups were also recorded.

After treatment，the NDSI scores and TCM syndrome scores for primary and secondary symptoms in the experimental group were lower than those in the control group.Post-treatment levels of MTL and GAS decreased while ghrelin levels increased in both groups，with the experimental group showing lower levels ofMTL and GASand higher levels of ghrelin compared to the control group.The SF-36 scores for physical functioning（PF），role-physical（RP），bodily pain（BP），vitality（VT）and social functioning（SF）increased after treatment in both groups，with higher increases observed in the experimental group.The BDIand BAIscores decreased in both groups post-treatment，with the experimental group showing lower scores than the control group.The scores in all four domains of the NDLQI and the total scores increased after treatment in both groups，with the experimental group showing higher scores than the control group（P<0.05）.Compared to the control group，the experimental group had a significantly higher number of safety cases（P<0.05）.

Huanglian Wendan Decoction combined with acupoint application is an effective treatment for dampness-heat of spleen and stomach type of FD，and its further clinical application is warranted.

To study the function of expelling water retention with drastic purgation of Jingdaji（Euphorbiae Pekinensis Radix，EPR）before and after being processed with vinegar on cancerous ascitesmodel rats.

Seventy-two SD male rats were random ly divided into 9 groups：the normal group，model group，positive group（furosemide），EPR and vinegar product groups at low，medium and high dose.Except the normal group，all other groups had intraperitoneal injection with Walker-256 ratascites cancer cells to replicate cancerous ascitesmodel rats.Positive group and each administration groups were orally administered with the corresponding powder，respectively.Normal group and model group were given the same amount of sodium carboxymethyl cellulose（0.5%）for 7 d.1 hour after the lastadministration，the amount of urine and ascites，and the contents of the renin-angiotensin-aldosterone system（RAAS）and antidiuretic hormones（ADH）in the serum were measured.Liver and kidney function indicators，oxidative damage indicators and pathological morphological changes in liver，stomach，intestine and kidney tissues were observed.

Compared with the normal group，the model group showed a significant decrease in urine output and a significant increase in ascites volume（P<0.01），which indicated themodelwas successfully set up.The levels of plasma renin activity（PRA），angiotensinⅡ（AngⅡ），aldosterone（ALD），ADH，creatinine（CRE），blood urea nitrogen（BUN）and the activities of aspartate aminotransferase（AST），alanine aminotransferase（ALT）and malondialdehyde（MDA）in the serum and tissues all significantly increased（P<0.01），while the contents of superoxide dismutase（SOD）and glutathione（GSH）were significantly decreased（P<0.01），also there were obvious tissue damage in liver，stomach，intestine and kidney.Compared with those in themodel group，the urine volume in each administration groups increased，ascites volume，the levels of PRA，AngⅡ，ALD，ADH，CRE and BUN，the activities of AST and ALT and the content of MDA in the serum and tissues decreased，and the contents of SOD and GSH increased.The injury of each administration groups reduced，and there was no significant difference between EPR raw and vinegar-processed product powders.

EPR before and after processing with vinegar have the effects of expelling water retention with drastic purgation，by stimulating the RAAS to eliminate ascites and alleviate oxidative damage of cancerous as cites model rats.

To analyze the clinical effect of Shenqi Fuzheng Kang’ai Decoction（参芪扶正抗癌汤）combined with bevacizumab in the treatment of advanced colorectal cancer with Qiand blood deficiency syndrome.

The clinical data of 98 patients with advanced colorectal cancer who were admitted to the Affiliated Hospital of Nantong University from January 2022 to January 2024 was retrospectively analyzed.According to the treatment methods，they were divided into the conventional group（48 cases）and the combined group（50 cases）.Both groups were treated with XELOX chemotherapy.The conventional group was treated with bevacizumab，and the combined group was treated with Shenqi Fuzheng Kang’ai Decoction on this basis.The TCM syndrome score，tumor markers，T lymphocyte subsets，chemotherapy tolerance and quality of life score，clinical efficacy，adverse reactions and survival analysis of the two groups were observed.

After treatment，the TCM syndrome score and the levels of carcinoembryonic antigen（CEA），carbohydrate antigen 125（CA125），alpha-fetoprotein（AFP）and cytokeratin 19 fragment antigen 21-1（CYFRA21-1）in the combined group were lower than those in the conventional group（P<0.05）.The levels of CD3⁺and CD4⁺，the ratio of CD4⁺/CD8⁺，the scores of Karnofsky performance scale（KPS）and World Health Organization Quality of Life-BREF（WHOQOL-BREF）and the total effective rate in the combined group were higher than those in the conventional group（P<0.05）.There was no significant difference in the total incidence of adverse reactions between the two groups（P>0.05）.The progression-free survival（PFS）of the combined group was longer than that of the conventional group（P<0.05）.

Shenqi Fuzheng Kang’ai Decoction combined with bevacizumab in the treatment of advanced colorectal cancer with Qi and blood deficiency syndrome can effectively relieve the clinical symptoms of patients，improve the immunity，reduce the levels of tumormarkers，improve the clinical efficacy and quality of life，prolong the survival of patients，and has high safety.

To establish the infrared fingerprint spectra of standard decoctions of Xiangfu（Cyperi Rhizoma）and its processed products，and quickly identify the standard decoctions of Xiangfu（Cyperi Rhizoma）and its processed products.

Infrared fingerprint spectra were established for the decoctions of Xiangfu（Cyperi Rhizoma），wine-processed Xiangfu（Cyperi Rhizoma），vinegar-processed Xiangfu（Cyperi Rhizoma）and four-processed Xiangfu（Cyperi Rhizoma）.The differences among the average infrared spectra of the decoctions of Xiangfu（Cyperi Rhizoma），wine-processed Xiangfu（Cyperi Rhizoma），vinegar-processed Xiangfu（Cyperi Rhizoma）and four-processed Xiangfu（Cyperi Rhizoma）were analyzed using OMNIC 9.2 software.The common peaks of Xiangfu（Cyperi Rhizoma）and its processed product decoctions were identified using Spectrum forWindow 3.02 software and the relative peak heights were calculated.The differences among the processed Xiangfu（Cyperi Rhizoma）decoctions were compared using principal component analysis，orthogonal partial least squares-discriminant analysis and clustering analysis.

A total of 12 common peaks were identified and standardized for the decoctions of Xiangfu（Cyperi Rhizoma），vinegar-processed Xiangfu（Cyperi Rhizoma），wine-processed Xiangfu（Cyperi Rhizoma）and four-processed Xiangfu（Cyperi Rhizoma）using baseline correction and normalization.The differences were obvious in the 1700-1000 cm^-1 waveband，and fourmain contribution wavebands were selected，including2929 cm^-1，1053 cm^-1，997 cm^-1 and 1639 cm^-1.The processed Xiangfu（Cyperi Rhizoma）decoctionswere all clustered into four categories through analysis.

There are obvious differences among the decoctions of Xiangfu（Cyperi Rhizoma），vinegar-processed Xiangfu（Cyperi Rhizoma），wine-processed Xiangfu（Cyperi Rhizoma）and four-processed Xiangfu（Cyperi Rhizoma），and they can be quickly identified using infrared spectroscopy.The method is fast，effective and accurate.

To investigate the correlation between different types of refractory peptic ulcer（RPU）and clinical manifestations，and to provide clinical basis for the prevention and treatment of this disease in modern medicine.

A crosssectional study was conducted to retrospectively collect the general data，clinical medical records，tongue and pulse conditions，TCM syndromes and other related factors of130 patients with RPU who were hospitalized in the Department of Gastroenterology of Zhengzhou People's Hospital from January 1，2020 to September30，2023.The collected data was statistically analyzed to explore the relationship between different types of RPU and clinical manifestations.

（1）There was no significant difference between the types of RPU and the distribution of tongue and pulse.（2）In terms of disease location syndrome elements，the mostusual disease location syndrome elements of RPU were heart and stomach（100.00%），the others were spleen（43.08%）and liver（30.00%）；in terms of disease nature syndrome elements，the most usual disease nature syndrome elements were spleen and stomach weakness（cold）syndrome（28.46%），followed by liver-stomach disharmony syndrome（21.54%），blood stasis in stomach collateral syndrome（16.15%），damp-heat in spleen and stomach syndrome（14.62%），stomach Yin deficiency syndrome（10.77%），liver-stomach heat stagnation syndrome（8.46%）.（3）There was no significant difference in hypertension，coronary heart disease and weight among the three groups（P>0.05）.There were significant differences in gender，Helicobacter pylori（Hp）infection，smoking history，diabetes，age，height and oral non-steroidal anti-inflammatory drugs（NSAIDs）（P<0.05）.Oralad ministration of NSAIDs is a risk factor for refractory compound ulcer and refractory duodenalulcer.Coronary heart disease，height and Hp infection are risk factors for refractory gastric ulcer.

The patients with Hp infection have a higher probability of refractory gastric ulcer.The patients with smoking history，diabetes and NSAIDs are more likely to develop refractory duodenal ulcer.Patients with different ages and heights are prone to RPU types.Oral administration of NSAIDs is a risk factor for refractory compound ulcer and refractory duodenal ulcer.Coronary heart disease，height and Hp infection are risk factors for RPU.

This study aims to explore the potential categories of five pattern personalities and analyze the differences of each category on the Big Five Personality.

A total of 963 volunteers were recruited to participate in the study.The survey was conducted using the Five Pattern Personalities Inventory and Big Five Personality Inventory.The latent categories of five pattern personalities were analyzed using latent profile analysis.

1）It was found that there were five latent categories of five pattern personalities：“low Yang-low Shaoyin-high Taiyin group”（12.56%），“low Yang-high Yin group”（27.73%），“high Yang-high Shaoyin low Taiyin group”（37.49%），“high Yang-high Yin group”（5.93%），“high Yanglow Yin group”（16.34%）.2）The five latent categories of five pattern personality exhibited significant differences in each dimension of the Big Five Personality（P<0.001）.The“low Yang group”（categories 1 and 2）demonstrated significantly lower levels of openness，extroversion and agreeableness compared to the“high Yang group”（categories 3 and 5）（P<0.001），while scoring higher on neuroticism than the“high Yang group”.Additionally，the rigor score for the“high Yang-high Shaoyin low Taiyin group”was significantly higher than other groups，whereas it was lower for the“low Yang-low Shaoyin high Taiyin group”.Furthermore，extraversion and agreeableness scores were lower for“high Yang-high Yin group”compared to“high Yang group”but higher than those for“low Yang group”；neuroticism scores were lower than those for“low Yang group”but higher than those for“high Yang group”as well.

There are five potentially categories of five pattern personalities.These different latent categories were significantly different on the Big Five Personality.

To investigate the effect of Zijinlong Tablets（紫金龙片）combined with intravenous immunoglobulin in the treatment of lower respiratory tract infection in the elderly.

Totally 302 elderly patients with lower respiratory tract infections admitted to the hospital from March 2021 to March 2023 were selected and randomly grouped，each with 151 cases.Based on conventional treatment，the control group was given intravenous immunoglobulin，and the study group received Zijinlong Tablets combined with intravenous immunoglobulin.Then the clinicalefficacy，ratio of forced expiratory volume in the firstsecond to forced vital capacity（FEV₁/FVC），peak expiratory flow（PEF），maximum voluntary ventilation（MVV），arterial partial pressure of oxygen（PaO₂），arterial partial pressure of carbon dioxide（PaCO₂），interleukin-6（IL-6），C-reactive protein（CRP），interleukin-1（IL-1），procalcitonin（PCT），immunoglobulin-（Ig）A，IgG，IgM and T-lymphocyte subsets（CD3⁺，CD4⁺，CD8⁺）were compared.

The study group reported a higher overall efficacy rate than the control group（93.38% vs85.43%，P<0.05）.An increase in PEF，MVV and FEV₁/FVC were observed in all patients after treatment，and the increase was more notable in the study group than those in the controlgroup（P<0.05）.An elevation in PaO₂ and a decrease PaCO₂ were detected in all patients，and both improvements were more remarkable in the study group than those in the control group（P<0.05）.After treatment，the levels of IL-6，CRP，IL-1 and PCT showed a decrease in all patients，and the three parameters were all lower in the study group than those in the control group（P<0.05）.The levels of IgA，IgG and IgM demonstrated an increase in all patients，and were all larger in the study group than those in the control group（P<0.05）.An elevation in CD3⁺and CD4⁺and a decrease CD8⁺were found in all patients，and both improvements were more obvious in the study group than those in the control group（P<0.05）.

The application of Zijinlong Tablets combined with intravenous immunoglobulin in the treatment of lower respiratory tract infection in the elderly can effectively improve the pulmonary function and arterial blood gas indexes，attenuate the cellular inflammatory response，ameliorate the body immune function and promote the recovery of the patients.

To explore the clinical efficacy of Jianpi Lishi Jiedu Decoction（健脾利湿解毒汤）combined with pidotimod in the treatment of low-grade squamous intraepithelial lesions（LSIL）of the cervix combined with high-risk human papillomavirus（HPV）infection.

A totalof130 cases of LSIL complicated with high-risk HPV infection admitted to the hospital from January 2022 to September 2023 were included and divided into two groups using the computer random number table method，with 65 cases in each group.The control group was treated with pidotimod，while the observation group was treated with Jianpi Lishi Jiedu Decoction on the basis of the control group.The treatment lasted for 3 months.The clinical efficacy of two groups，the traditional Chinese medicine syndrome score and serum inflammatory factor levels［high-sensitivity C-reactive protein（hs-CRP），interleukin-12（IL-12）and tumor necrosis factorα（TNF）-α）］and changes in viral load were compared.

①The total effective rate of the observation group was 93.85%，which was higher than that（81.54%）of the control group（χ²＝4.561，P＝0.033）.②The difference in traditional Chinese medicine syndrome scores for vaginal discharge，itching，fatigue，edema and loose stools in the observation group was lower than that in the control group［（1.13±0.13）points vs.（2.05±0.16）points，（1.09±0.11）points vs.（1.65±0.15）points，（1.01±0.19）points vs.（1.66±0.22）points，（0.68±0.17）points vs.（1.13±0.19）points，（0.89±0.18）points vs.（1.38±0.14）points］（t＝35.979～17.324，all P<0.01）.③The serum levels of TNF-α and hs-CRP of the observation group were all lower than those of the control group［（1.13±0.36）pg/mL vs.（1.72±0.41）pg/mL，（2.19±0.45）mg/L vs.（4.24±0.87）mg/L］（t＝8.718-16.874，all P<0.01），and the serum IL-12 levels were higher than the control group［（16.52±5.03）pg/mL vs.（14.25±5.16）pg/mL］（t＝-2.540，P＝0.006）.④The recurrence rate［4.61%（3/65）］in the observation group was significantly lower than that［16.92%（11/65）］in the control group（χ²＝5.123，P＝0.024）.The HPV viral load in the observation group was lower than that in the control group［（1.85±0.59）Igcopies/mL vs.（2.73±0.65）Igcopies/mL］（t＝-8.082，P<0.01），and the seroconversion rate was higher than that in the control group（69.23% vs.43.08%）（χ² ＝9.029，P＝0.003）.

The combination of Jianpi Lishi Jiedu Decoction and pidotimod in the treatment of LSIL complicated with high-risk HPV infection has a definite therapeutic effect.It can restore the patient's immune inflammatory balance and reduce the HPV viral load.

To observe the clinical effect and safety of Chaipo Decoction（柴朴汤）combined with Sulidi in the treatment of bronchial asthma.

A total of 92 patients with bronchial asthma admitted from March 2022 to March 2024 were included in this study.The patients were divided into two groups by random number table method，namely，the Sulidiere group receiving Sulidiere treatment and the Chaipo Decoction group receiving Sulidiere treatment combined with Chaipo Decoction，with 46 cases in each group.The patient data of each group was observed，including the clinical efficacy，adverse reactions and TCM symptom score before and after treatment（sleeping at night and waking up in the morning with severe cough，dry cough with little or no phlegm，rib distension，restlessness and chest tightness，and discomfort in the throat，etc.）and sputum inflammatory factor index changes，pulmonary function index and index of immune function changes，asthma control test（ACT）and Mini Asthma Quality of Life Questionnaire（Mini AQLQ）.

The total effective rate of the Chaipo Decoction group was higher than that of the Sulidiere group（P<0.05）.The adverse reaction rate of patients in Chaipo Decoction group was similar to that in Sulidiere group（P>0.05）.Before treatment，the patients in both groups received traditional Chinese medicine syndrome scores（sleeping at night and waking up in themorning with severe cough，dry cough with little or no phlegm，rib distension，restlessness and chest tightness，and discomfort in the throat，etc.），interleuin 6（IL-6），interleuin 17（IL-17），tumour necrosis factor-α（TNF-α）and other indicators of sputum factors，forced vital capacity（FVC）and forced expiratory volume in one second（FEV1），percentage of forced expiratory volume at the first second to forced vital capacity（FEV1%）and other pulmonary function indicators，CD3⁺，CD4⁺，CD8⁺，CD4⁺/CD8⁺and other immune function indicators，ACT and Mini AQLQ scores were not significant（P>0.05）.After treatment，TCM syndrome scores（coughing up atnight and in the morning，dry cough with little or no sputum，hypochthmic fullness，upset chest tightness，pharyngeal distress，etc.），sputum inflammatory factor indexes（IL-6，IL-17，TNF-α），lung function indexes（FVC，FEV1，FEV1%），and immune function indexes（CD3⁺，CD4⁺，CD8⁺，CD4⁺/CD8⁺）of all patients in each group，ACT and Mini AQLQ scores were improved.After treatment，patients in the Chaipo Decoction group had TCM syndrome scores（sleeping at night and waking up in the morning with severe cough，dry cough with little or no phlegm，rib distension，restlessness and chest tightness，and discomfort in the throat，etc.），sputum inflammatory factor indexes（IL-6，IL-17，TNF-α），lung function indexes（FVC，FEV1，FEV1%）and immune function indexes（CD3⁺，CD4⁺，CD8⁺，CD4⁺/CD8⁺），ACT and Mini AQLQ scores were better than those in the Seridial group（P<0.05）.

The clinical effect of Chaipo Decoction combined with Sulidi in the treatment of bronchial asthma is good.The inflammation of the patients is significantly improved.The symptoms and lung function are improved.The disease is controlled.The quality of life is improved.The adverse reactions are less.The treatment is relatively safe and reliable.

To investigate the efficacy and safety of Sanhuangtangshenkang（三黄糖肾康）combined with Dapagliflozin in the treatment of Qi deficiency and blood stasis type of early diabetic nephropathy（DN）.

A randomized，double-blind and placebo-controlled clinical trial was conducted involving 120 patients from the Endocrinology and Nephrology Departments at Taizhou Hospital of Traditional Chinese Medicine from June 2021 to June 2023.The participants were allocated into two groups.The experimental group（60 patients）received standard care augmented with Sanhuangtangshenkang and Dapagliflozin，while the control group（60 patients）was treated with standard care plus placebo granules and Dapagliflozin.The duration of treatmentwas 12 weeks.The primary endpoints included the efficacy of treatment，the renal function，the inflammatory markers，the enhancement of traditional Chinese medicine（TCM）syndromes，and the frequency of adverse events.

A total of 117 patients were successfully enrolled，59 in the experimental group and 58 in the control group.The experimental group showed a significantly higher overall effective rate compared to the control group（98.30% versus70.68%，P<0.05）.Biochemical indicators such as the urine albumin-to-creatinine ratio（UACR），24-hour urinary microalbumin（UMA）and 24-hour urinary protein were significantly improved in the experimental group compared to those in the control group after 12 weeks of treatment（P<0.05）.Inflammatory markers including serum amyloid A（SAA），interleukin-6（IL-6），C-reactive protein（CRP）and homocysteine（Hcy）also showed more significant reductions in the experimental group，especially after 12 weeks of treatment（P<0.05）.The improvements in renal function and other biochemical indicators，such as glycated hemoglobin（HbA1c），estimated glomerular filtration rate（eGFR）and total cholesterol（TC），were also notably better in the experimental group（P<0.05）.TCM symptom scores further confirmed the improvement in various indicators in the experimental group after treatment（P<0.05）.Although there was no statistically significant difference in the incidence of adverse reactions between the two groups（P>0.05）.The experimental group exhibited a lower rate of adverse reactions（3.38%）compared to the control group（8.62%）.

The combination of Sanhuangtangshenkang with Dapagliflozin is highly effective and safe for improving renal function，glycemic control and TCM syndromes in patients with early-stage DN characterized by Qi deficiency and blood stasis，demonstrating both enhanced safety and superior efficacy.

To explore the intervention and mechanism of YiqiBushen Tongluo Decoction（益气补肾通络方）in the treatment of varicocele infertility inmice.

A total of60 Sprague Dawley（SD）rats were selected and divided into sham operation group，model group，Yiqi Bushen Tongluo Decoction low dose group，medium dose group and high dose group according to random number table method，with 12 cases in each group.The drug was given by intragastric administration 48 h after the modeling was completed and continuously used for60 d.The testis and epididymis quality，sperm quality，spermatogenic cell apoptosis index and the levels of malondialdehyde（MDA），superoxide dismutase（SOD）and nitric oxide（NO）in testicular tissue were compared among all groups.

The testis quality，epididymal quality，sperm concentration and sperm motility in the model group were lower than those in the sham operation group，and the spermatogenic apoptosis index was higher than those in the sham operation group，with statistical significance（P<0.05）.Compared with those in the model group，the testis quality，epididymal quality，sperm concentration and sperm motility were significantly increased in the Yiqi Bushen Tongluo Decoction low dose，medium dose and high dose groups，and the spermatogenic apoptosis indexwas significantly decreased，with statistical significance（P<0.05）.The levels of MDA and NO in the model group were higher than those in the sham operation group，and the SOD level was lower than that in the sham operation group，with statistical significance（P<0.05）.Compared with those in the model group，the levels of MDA and NO were significantly decreased in the Yiqi Bushen Tongluo Decoction low dose，medium dose and high dose groups，and the SOD level was significantly increased，with statistical significance（P<0.05）.

Yiqi Bushen Tongluo Decoction can improve the sperm quality of mice with varicocele infertility.Itsmechanism of action is mainly related to inhibiting spermatogenic cell apoptosis，anti-oxidative stress and reducing NO level.