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Outline
To observe the anti-inflammatory effect of Fuyanshu Capsule（妇炎舒胶囊, FYS）on sequelae of pelvic inflammatory disease（SPID）model rats on Krüppel-like factor 4（KLF4）, Toll-like receptor 2（TLR2）, nuclear factor-kappa B（NF-κB）and myeloid differentiation factor 88（MyD88）.
Thirty-six rats were randomly divided into blank group, model group, Kangfuyan Capsule（康妇炎胶囊）group（KFY group）, FYS high-dose group, FYS medium-dose group, FYS low-dose group. The SPID animal model was established by the method of mixed bacterial liquid injection and mechanical injury. The blank group was treated with distilled water by gavage, and the other groups were given the corresponding drug suspension by gavage for 21 days. HE staining was used to observe the morphology of uterine tissue and score the pathological sections. The mRNA expressions of TLR2, MyD88 and KLF4 were determined by RT-PCR, the expression of NF-κB was observed by immunohistochemistry method, and the relative protein expressions of TLR2, MyD88 and KLF4 in uterine tissues of each group were determined by Western Blot. The correlation between KLF4 and key factors of TLR2/MyD88/NF-κB pathway was analyzed.
Compared with those in the model group, the scores of uterine tissue inflammation in KFY group, FYS medium - and high-dose groups were significantly decreased（P<0.05）. KLF4 protein in KFY group and FYS high-dose group was significantly increased（P<0.01）. The protein and mRNA of TLR2 in KFY group and FYS high-dose group were significantly decreased（P<0.05）. MyD88 protein in KFY group, FYS high-and medium-dose groups was significantly decreased（P<0.05）, and MyD88 mRNA in KFY group and FYS high-dose group was significantly decreased（P<0.05）. The expressions of NF-κB protein in KFY group, FYS medium - and high-dose groups were significantly decreased（P<0.05）. The expression of KLF4 protein was negatively correlated with the expressions of TLR2 protein, MyD88 protein and NF-κB protein（P<0.05）.
FYS can inhibit the TLR2/MyD88/NF-κB pathway by up-regulating KLF4 expression.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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