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The aim of this study was to investigate the effects of myristicin on the regulation of endonuclease/exonuclease/phosphatase family domain containing 1（EEPD1）expression and its subsequent impact on the proliferation, migration, invasion and apoptosis of pulmonary squamous cell carcinoma.
Molecular docking techniques were employed to analyze the interaction between myristicin and the target protein EEPD1. Immunohistochemistry method was used to assess the levels of EEPD1 protein in lung squamous carcinoma and adjacent paracancerous tissues on tissue chips. The gene expression activity in cell lines was evaluated using real-time quantitative polymerase chain reaction（qRT-PCR）, while the effect of myristicin on the proliferation of lung squamous carcinoma cells KNS-62 and NCI-H226 was assessed with Cell Counting Kit-8（CCK-8）. Scratch assay and Transwell migration and invasion experiments were conducted to examine the impact of myristicin on the migration and invasion capabilities of lung squamous carcinoma cells. Flow cytometry method was utilized to determine the influence of myristicin on the apoptosis of lung squamous carcinoma cells, and Western blot（WB）was employed to analyze the expression of EEPD1 protein in these cells.
Myristicin could form specific binding with the EEPD1 protein. Higher EEPD1 positive signal intensity was observed in lung squamous carcinoma tissue samples compared to adjacent paracancerous tissue control areas. CCK-8 assay results demonstrated that myristicin significantly inhibited the proliferation of lung squamous carcinoma cells. A slower scratch healing rate suggested that myristicin could inhibit the migration of lung squamous carcinoma cells. Transwell assay results similarly showed that myristicin could inhibit the migration and invasion of lung squamous carcinoma cells. Flow cytometry data revealed that myristicin promoted the apoptosis of lung squamous carcinoma cells, while WB analysis indicated that myristicin reduced the expression level of EEPD1 protein.
In vitro experimental evidence supports that myristicin exerts its anticancer effects by down-regulating the expression of EEPD1 protein, specifically manifested in inhibiting the proliferation, migration and invasion of pulmonary squamous cell carcinoma and promoting cell apoptosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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