To observe the effectof herbal cake separatedmoxibustion on themethylation of ignaling lymphocytic activation molecule（SLAM）and poliovirus related receptor 2 gene（PVRL2）and the costimulatory molecule［tumor necrosis factor superfamily member 9（4-1BB）］in immunosuppressed rats.

Fifty SD ratswere random ly divided into blank group，immunosuppressivemodel group，herbal cake separated moxibustion group，fake cakemoxibustion group and Chinese herbalmedicine gavage group，with 10 rats in each group. The modelwasmade by intraperitoneal injection of cyclophosphamide，and the other groups except the blank group set up themodelwith the dose of 30 mg/kg for 3 days.“Shenque（RN8）”“Guanyuan（RN4）”“Zhongwan（RN12）”and“ Zusanli（ST36）（double）”were selected for intervention in the herbal cake separated moxibustion group and the fake cakemoxibustion group for 10 consecutive days. The Chinese herbalmedicine gavage group was given Liuwei Dihuang Decoction（六味地黄汤）suspension for 10 days. The number of white blood cells（WBC）in each group was detected by blood routine，and the contents of CD28，4-1BB，tumor necrosis factor ligand superfamily member 9（4-1BBL）and interleukin-17（IL-17）in serum were detected by ELISA. The average fluorescence intensity of4-1BB and 4-1BBL in spleen tissue was detected by immunofluorescence. BSP method was used to detect SLAM and PVRL2.

The blood routine results showed that compared with those of the blank group，the white blood cells in the model group were significantly reduced. ELISA results showed that compared with those of the blank group，the levels of CD28，4-1BB and IL-17 in the immunosuppressive model group were significantly increased. Compared with those of the immunosuppressivemodel group，the levels of CD28，4-1BB and IL-17 in the herbal cake separatedmoxibustion group were significantly decreased. The results of immunofluorescence showed that the average fluorescence intensity of 4-1BB and 4-1BBL in the mmunosuppressivemodel group was significantly higher than that in the blank group. Compared with those of the model group，the fluorescence intensities of 4-1BB and 4-1BBL in the herbal cake separated moxibustion group were significantly decreased. The methylation results showed that compared with those of the blank group，the methylation rates of SLAM and PVRL2 promoter regions in the mmunosuppressivemodel group were decreased. Compared with those of themodel group，themethylation rate of SLAM in the herbal cake separated moxibustion group increased，but the methylation rate of PVRL2 decreased.

Herbal cake separated moxibustion may improve the immunosuppressive state by regulating the methylation level of costimulatorymolecule 4-1BB and its ligand as well as SLAM，thereby significantly improving the immune function.

Through in vitro studies，it explored whether Supplemented Buyang Huanwu Decoction（加味补阳还五汤，SBHD）could promote adipose stem cells（ADSCs）in greater omentum to paracrinehepatocyte growth factor（HGF）and whether this effect could inhibit the epithelial-mesenchymal transition（EMT）of peritonealmesothelial cells（PMC）in order to initially clarify the pharmacological mechanism and target of this prescription in preventing and treating postoperative abdominal adhesion（PAA）.

Experiment 1 clarified whether the inhibitory effect of SBHD on the EMT of PMCwas a directeffectof the formula on PMC or an indirect effect by regulating the paracrine secretion of ADSCs in the greater omentum. After culturing ADSCs with SBHD，conditional culture media（hereinafter referred to as“Chinese medicine culture media”）were prepared to intervene in the PMC of EMTmodels，and the expression levels of EMT related proteins E-cadherin（E-Cad）andα-smooth muscle actin（α-SMA）were detected by Western Blot. Experiment 2 clarified whether the inhibitory effectof SBHD on EMT of PMC was related to the secretion of HGF by ADSCs in the greater omentum. The intervention of PMC used three types ofmedia：low HGF expressing ADSCs conditioned medium（hereinafter referred to as “low HGFmedium”），normal ADSCs conditioned medium（hereinafter referred to as“normal medium”）and traditional Chinese medicine culture medium and the following indicators were detected.（1）ELISA was used to detect the content of HGF in the three media before and after intervention in PMC.（2）Western Blot，qPCR and IFmethods were used to detect the expression levels of EMT related proteins in each group of PMCs after intervention，and scratch assay was used to detect the migration ability of PMCs in each group.

Experiment 1：Effect of SBHD and traditional Chinese medicine culture medium on EMT related indicators：Compared with the model group，the SBHD group up-regulated the protein expression of E-Cad（P<0.05）and down-regulated the protein expression of α-SMA（P<0.05）. The traditional Chinese medicine culture medium group significantly up-regulated the protein expression of E-Cad（P<0.01）and significantly down-regulated the protein expression of α-SMA（P<0.01）. Compared with the SBHD group，the traditional Chinese medicine culture medium group up-regulated the protein expression of E-Cad（P<0.05）and significantly downregulated the protein expression of α-SMA（P<0.01）. Experiment 2：（1）Comparison of HGF contents in three different culture media before and after acting on PMC：Before acting on PMC，compared with the normal medium，the content in low HGF medium was significantly reduced（P<0.01），while the content in traditional Chinese medicine culture medium was significantly increased（P<0.01）. After acting on PMC，the content of each culture medium group significantly decreased.（2）Expression of EMT-related proteins：Based on the results of Western Blot，qPCR and IF，compared with the model group，all culture medium groups were able to increase E-Cad expression（P<0.05 or P<0.01）and decrease the expressions of α-SMA and vimentin（VIM）（P<0.05 or P<0.01）. All culture medium groups were able to inhibit the EMT of PMC，with the order of effect being Chinese medicine culture medium group>normal medium group>low HGF medium group.（3）Changes in cellmigration ability of EMT：After intervention for 24 h and 48 h，the healing rate of each group was significantly decreased compared with that of the model group（P<0.01）. Each group of culturemedia can reduce the Rmigration ability of the EMT model，with the order of effect being Chinese medicine culture medium group>normal medium group>low HGF medium group.（4）Correlation analysis of EMT-related protein expression and HGF content：The expression level of E-Cad protein was positively correlated with the content of HGF（P<0.05）. The protein expressions of α-SMA and VIM were negatively correlated with the content of HGF（P<0.05 or P<0.01）.

The therapeutic effect of SBHD in inhibiting the EMT of PMC and preventing PAA may be multi-target，with both direct effects on PMC and indirect effects by enhancing the paracrine secretion of HGF by ADSCs in the greateromentum，with the latter beingmore important.

To lay the literature foundation for the study of the processing mechanism of Shengma（Cimicifugae Rhizoma，CR）by clarifying the origin of the theory of “different use of raw and processing products”and sorting out the experience of ancient and modern use and modern research progress.

By systematically consulting relatedmateriamedica works and ancient and modern medical records，it excavated the origin track of “differentuse of raw and processing products”on the clinical experience，the process of theory formation and the relationship with Chinese medicine processing. The historical evolution on the method of processing and the clinicalexperience of CR were systematically reviewed through the research of materiamedica. And the modern research situation of “differentuse of raw and processing products”of CR was summarized through reviewing the literature.

The experience of “different use of raw and processing products” first appeared in the Eastern Han Dynasty，and its theory was formed in the Jin and Yuan Dynasty. Furthermore，the theory of “differentuse of raw and processing products” was used to guide the processing of Chinese medicine in the Ming and Qing Dynasties，which expanded the varieties of Chinese medicine decoction pieces and enriched the processing theory of Chinese medicine. There are various processing methods of CR，including honey-frying，oven earth-frying，yellow rice wine-frying，etc.，and there are differences use among processed products of CR：raw products for detoxification and processing-products for rising Yang.

CR has a long history of clinical use in the aspect of differentuse of raw and processing products. It is helpful to deepen the understanding of the scientific connotation of the theory of “differentuse of raw and processing products” in Chinese medicine processing to accelerate the research of material basis and processing mechanism of CR. This review excavated and sorted out the theoretical origin track of “different use of raw and processing products” and the using experience and modern research progress of CR，which provided references for the accurate understanding of the theory of “differentuse of raw and processing products” of Chinese medicine，the using characteristics of CR based on“different use of raw and processing products”，and the rational clinical application of different decoction of CR.

To improve the genetic algorithm and innovate the recommendation model，mine the core prescriptions and recommend the effective prescriptions based on the electronic medical records data of type 2 diabetes mellitus（T2DM）.

Based on the real-world electronic medical records data，the database of effective prescriptions of T2DM patients and original prescriptions of different syndromes was constructed. The genetic algorithm was improved and the construction of fitness function was optimized，so the extracted core effective prescriptions can evolve towards the collection of effective prescriptions. Based on the similarity relationship between the core effective prescriptions and the collection of original prescriptions，the recommendation model was innovated，and the prescription recommendation was mined by traversing the collection of original prescriptions of different syndromes.

A total of 17 712 prescriptions with effective diagnosis and treatment standards were used，and 37 kinds of traditional Chinese medicines were extracted. Finally，26 prescriptions with “recommendation value≥85%” were excavated，and the maximal recommendation value was 97.26%.

In this study，the improved genetic algorithm has improved the ability of feature extraction and global search for prescription sets of traditional Chinese medicine，and proposed a new recommendation model for clinical prescriptions decision-making，thus improving the utilization rate of electronic medical records data.

To observe the effect of Yiqi Congming Decoction（益气聪明汤）combined with acupuncture on dizziness caused by deficiency of Qi and blood.

A total of 100 cases of vertigo with deficiency of Qi and blood in the hospital from January 2020 to January 2023 were selected as research objects. By simple random number table，they were divided into observation group and control group with 50 cases in each group. Both groups received acupuncture treatment for 4 weeks，and the observation group was treated with Yiqi Congming Decoction for 4 weeks. Maximum vertebrobasilar blood systolic flow velocity，the levels of serum total cholesterol（TC）and fibrinogen（Fib）of the two groups were compared before and after treatment，and the vertigo scores of the two groups were compared before and after treatment. At the end of treatment，the therapeutic efficacy of the two groups and the occurrence of adverse reactions during treatment were compared according to the relevant standards of the Guiding Principles for Clinical Research of New Chinese Medicine Drugs.

The clinical recovery rates of the observation group and the control group were 38.00%（19/50）and 18.00%（9/50），respectively，and the difference was statistically significant（P<0.05）. After treatment，the levels of serum TC and Fib in the two groups were lower than those before treatment，and the levels of the observation group were lower than those of the control group. The difference was statistically significant（P<0.05）. After treatment，the maximum systolic flow velocities of left vertebral artery，right vertebral artery and basilar artery in both groups were significantly higher than those before treatment，and the values in the observation group were significantly higher than those of the control group. The difference was statistically significant（P<0.05）. After treatment，the vertigo score in both groups was higher than that before treatment，and the score of the observation group was significantly higher than that of the control group. The difference was statistically significant（P<0.05）. No adverse reactions occurred in both groups during treatment.

Yiqi Congming Decoction combined with acupuncture in the treatment of vertigo with deficiency of Qi and blood has better effect than acupuncture alone. It can significantly improve the maximum systolic flow velocity of vertebra-basilar artery in patients and has high safety，which is suitable for promotion.

To explore the molecular mechanism ofWendan Decoction（温胆汤）improving cognitive impairment in schizophrenia from the perspective of neuronal synaptic plasticity by using glutamate-induced HT22 cells as a cellularmodel of schizophrenia and detecting synaptic plasticity related proteins，postsynaptic density protein-95（PSD-95），growth associated protein-43（GAP-43），c-Jun NH2-terminal kinase-interacting protein-3（JIP-3）and connexin-43（Cx-43）.

The SD ratswere randomly divided into four groups：Wendan Decoction 40 g/kg，30 g/kg and 20 g/kg groups and clozapine 0.02 g/kg group. Each drug group was given the corresponding dose of drug by gavage，once a day. After seven days，the ratswere be killed to collect blood and prepare the drug-containing serum. CCK8 assay was used to determine the concentration and time point of glutamate action on astrocytes. HT22 cells at 10 mmol/L glutamate for 24 h were used as a cellmodel to detect the effect of Wendan Decoction-containing serum on the apoptosis rate of HT22 cells by flow cytometry. RT-qPCR was used to detect the expressions of Psd95，Gap43，Jip3 and Cx43 mRNA in HT22 cells. The expressions of PSD-95，GAP-43，JIP-3 and Cx-43 proteins in HT22 cellswere detected by Western blot.

According to the results of the effectof different concentrations of glutamate on the proliferation rate of HT22 cells，10 mmol/L glutamate HT22 cells for 24 h were determined as the cell experimental conditions. Compared with thatof the normal control group，the apoptosis rate of HT22 cells in the glutamate model group increased significantly. The mRNA and protein expressions of PSD-95，GAP-43，JIP-3 and Cx-43 were also significantly down-regulated（P<0.01）. Compared with that of the glutamate model group，the apoptosis rate of HT22 cells in Wendan Decoction-containing serum groups were significantly decreased and the expressions of PSD-95，GAP-43，JIP-3，Cx-43 mRNA and protein significantly up-regulated（P<0.05 or P<0.01）.

Wendan Decoction may be to reduce apoptosis in neurons and up-regulate the expressions of PSD-95，GAP-43，JIP-3 and Cx-43，and then regulate synaptic plasticity，in order to effectively improve cognitive deficits in schizophrenia.

To explore the effects of Modified Zhibai Dihuang Pill（知柏地黄丸）combined with acupoint catgutembedding on sex hormones and plasma Kisspeptin levels in girls with idiopathic central precocious puberty.

Seventy-two girls with idiopathic central precocious puberty who received treatment in the hospital from June 2021 to December 2022 were selected as subjects of this study，and were randomly divided into observation group and control group according to random number table method，with 36 cases in each group. The observation group was treated with Modified Zhibai Dihuang Pill combined with acupoint catgut embedding. The control group was treated with lepraline acetate 3.75mg by subcutaneous injection. Growth and development indexes，secondary sex characteristics，sex hormone levels and plasma Kisspept in levels were compared between the two groups.

There were no significant differences in heightand weightbet ween two groups before and after treatment（P>0.05）. The bone age index between two groups before treatment had no significance（P>0.05）. The bone age indexes in the two groups after treatment were significantly lower than those before treatment，and the index in the observation group was significantly lower than that in the control group（P<0.05）. There were no significantdifferences in the diameter of milk nucleus，uterine volume，ovarian volume ormaximum follicle diameter between the two groups before treatment（P>0.05）. After treatment，the diameter ofmilk nucleus，uterine volume，ovarian volume and maximum follicle diameter between the two groups were significantly lower than those before treatment，and the values in the observation group wre significantly lower than those in the control group（P<0.05）. There were no significant differences in the levels of follicular estrogen（FSH），estradiol（E₂）or luteinizing hormone（LH）between the two groups before treatment（P>0.05），and the levels in the two groups after treatment were significantly lower than those before treatment. There was no significant difference in plasma Kisspeptin level between the two groups before treatment（P>0.05），and the plasma Kisspeptin level in the two groups after treatment was significantly lower than that before treatment，and that in the observation group was significantly lower than that in the control group（P<0.05）.

In the treatment of idiopathic central precocious puberty in girls，Zhibai Dihuang Pill combined with acupoint catgut embedding can effectively improve the growth，development and secondary sex characteristics of the children，and reduce the secretion of sex hormones and plasma Kisspeptin.

To observe the differences in biological traits between male and female Fangji（Stephania tetrandra）and to screen and identify simple sequence repeats（SSR）loci，so as to provide methods and basis for clarifying the differences bet ween male and female Fangji（Stephania tetrandra）and identifying the sexes of Fangji（Stephania tetrandra）in the early stage.

The differences in biological traitswere observed and analyzed by using the artificial ex-situ planting for 4 years as experimental materials，and the SSR marker identification technology was used to dig out and verify the specific SSR loci ofmale and female Fangji（Stephania tetrandra），and the SSR marker sites that can be used for early identification ofmale and female Fangji（Stephania tetrandra）were screened and obtained.

There was no significant difference in the phenotypic traits of female and male Fangji（Stephania tetrandra）at the 0.05 significant level，and there was no significant difference in the diameter，surface color or cross-section color of male and female Fangji（Stephania tetrandra），but the diameter，surface color or cross-section color of of female Fangji（Stephania tetrandra）were lighter and more brittle and powdery than those of male Fangji（Stephania tetrandra）. A total of 38 956marker sites were obtained by mining SSRmolecular marker sites with MISA，including 25 632 loci formale plants and 30 389 loci for female plants.

There were some differences in the phenotypic traits of male and female Fangji（Stephania tetrandra）and the traits of medicinal materials，but there was no significant difference in the overall quality traits. The SSR molecular marker differential loci screened in this study can identify the sex of Fangji（Stephania tetrandra）at an early stage，which can provide an effective basis for the development of Fangji（Stephania tetrandra）planting industry.

To observe the protective effect of Jiedu Huayu Granules（解毒化瘀颗粒）on liver failure in rats and to investigate its mechanism of action.

Thirty SPF-grade Wistar rats were divided into blank control group，model control group and Jiedu Huayu Granules group（JDHYKL group）according to the number table method，with 10 rats in each group. Dgalactosamine（D-GalN）+lipopolysaccharide（LPS）was used to replicate the ratmodel of acute liver failure，and the JDHYKL group was given Jiedu Huayu Granules by irrigated 5 days beforemodeling and continued until 24 h after modeling，and the blank group and the model control group were given equal amounts of distilled water. The liver function［alanine aminotransferase（ALT），aspartate aminotransferase（AST），total bilirubin（TBil）］，the levels of oxidative stress indexes［malondialdehyde（MDA），superoxide dismutase（SOD），glutathione peroxidase（GSH-PX）］，inflammatory cytokines［interleukin-6（IL-6）and tumor necrosis factorα（TNF-α）］were detected in each group，and the histopathological changes of liver were observed in each group by HE staining.

The hepatocyte necrosis in the liver tissue of rats was significantly improved after treatment with Jiedu Huayu Granules. Meanwhile，it could significantly decrease the levels of ALT，AST，TBil，MDA，IL-6 and TNF-α，and increase the levels of SOD and GSH-PX（P<0.05）.

Jiedu Huayu Granules have significant antioxidant and antiinflammatory effects，and could mitigate hepatocyte necrosis in liver failure.

To investigate the mechanism of berberine on energy metabolism and related pathways in EC9706 cells.

The cellswere routinely cultured，MTT screened，and two mass concentrations of 15 μg/mL and 30 μg/mL were selected. The cell cycle and apoptosis were detected by flow cytometry，the glycolytic potential and mitochondrial respiratory capacity were detected by energy metabolism analysis system，and the changes in the expression of phosphorylated adenylate activated protein kinase（p-AMPK）/adenylate activated protein kinase（AMPK）and the downstream phosphor ylated mammalian target of rapamyc in（p-mTOR）/mammalian target of rapamycin（mTOR）proteins were explored by Western Blotting. The expression levels of AMPK and downstream mTOR were verified at the gene level by quantitative real-time PCR（qPCR）.

Berberine significantly inhibited the proliferation of EC9706 cells（P<0.01）and promoted the apoptosis of EC9706 cells（P<0.05）. Compared with the control group，berberine and drug group showed a significant decrease in the G0/G1 phase（P<0.05），and a significant increase in the proportion of Sphase（P<0.01）. Berberine inhibited the glycolytic potentia and mitochondrial respiration of EC9706 cells（P<0.01）in a concentration-dependent manner，and berberine promoted p-AMPK/AMPK（P<0.01）and inhibited the expression of p-mTOR/mTOR protein（P<0.05）. At the gene level，berberine promoted AMPK expression（P<0.05）as well asmTOR expression（P<0.01）.

Berberine can inhibit the proliferation of oesophageal cancer EC9706 cells through energy metabolic pathway，promote apoptosis of oesophageal cancer cells and hinder their division.

To explore whether Sagittaria Sagittifolia polysaccharide（SSP）plays a protective role in L02 hepatocyte injured by six heavy metals mixture bymediating the Toll-like receptors 4（TLR4）/myeloid differentiation factor 88（MyD88）/nuclear transcription factor-κB（NF-κB）signaling pathway.

Firstly，according to the dietary intake of six heavy metals in rice sold in Beijing，a heavy metal ixture was prepared for inducing L02 cell injury. MTT assay was used to detect the effect of SSP（0.25 mg/mL，0.5 mg/mL，1 mg/mL，2 mg/mL）on the L02 cells survival rate with 24 h pre-treatment. Annexinv FITC/propidium iodide（PI）double staining combined with flow cytometry and Hoechst 33342 staining were used for detecting the cell apoptosis. The content of reactive oxygen species（ROS）in cells was detected by using DCFH-DA fluorescence probe. The protein expressions of poly-ADP ribose polymerase1（PARP1），dynamic related protein 1（Drp1），tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β），interleukin-17（IL-17），TLR4，MyD88 and NF-κB p65 were detected by Western blot.

MTT assay showed that SSP（1 mg/mL，24 h）had the best protective effect. Compared with those of the model group，the ROS contentand apoptosis rate in SSP treatmentgroup were significantly decreased（P<0.05），and the protein expressions of Drp 1，TNF-α，IL-1β，IL-17，TLR4，MyD88 and NF-κB p65 decreased significantly（P<0.05）and the protein expression of PARP1 increased significantly（P<0.01）.

SSP can effectively improve the apoptosis of L02 cells induced by six heavy metals mixture，whichmay be related to the regulation of TLR4/MyD88/NF-κB signaling pathway to inhibit the oxidative damage and inflammatory response.

To study the molecular mechanism of Liujunzi Decoction（六君子汤）to improve C2C12 myoblast differentiation in tumor environment，providing experimental basis for the treatment of tumor cachexiamuscle atrophy with strengthening spleen and harmonizing stomachmethod.

It induced the differentiation of myoblasts C2C12 and observed the length of the differentiated myotube with an inverted microscope. Myoblasts C2C12 were co-cultured with lung cancer cell Lewis，and then were divided into blank group，model group，Fork head box protein O1（FoxO1）inhibitor group，Liujunzi Decoction（六君子汤）group and combination group. Western Blot detected the expression of myogenic differentiation antigen（MyoD）in C2C12 cells and the expressions of proteins associated with the peroxisome proliferator-activated receptor gamma coactivator 1-alpha（PGC-1α）/FoxO1 pathway. Electron microscopy observed the changes in the number of mitochondria and ultrastructure of C2C12 cells. Flow cytometry observed the changes inmitochondrial membrane potential of C2C12 cells. Quantitative real-time PCR detected mRNA in the PGC-1α/FoxO1 pathway in C2C12 cells.

Experiments showed that C2C12 cells had the longest transverse diameter in the muscle tube after 48 h of differentiation. Compared with those in the blank group，the MyoD protein expression and mitochondria number were significantly reduced in the model group（P<0.05）. The wrinkled boundary of themitochondrial bilayermembrane was not clear or even more C-shaped or stromal swelling，and the number of mitochondrial cristae was reduced. The arrangementwas disordered，fractured or dissolution disappeared into vacuoles. The mitochondrial membrane potential was decreased. The apoptotic ratewas significantly increased（P<0.05）. The mRNA expression ofmitochondrial transcription factor A（tFAM）was significantly reduced，and FoxO1 protein expression was significantly increased. The protein expressions of PGC-1α，FTO，mTOR and tFAM were significantly decreased（P<0.05）. Compared with themodel group，the expression of MyoD protein increased significantly in Liujunzi Decoction group（P<0.05）. In FoxO1 inhibitor group，Liujunzi Decoction group and the combination group，the number of mitochondria was significantly increased（P<0.05），the mitochondrial bilayer outermembrane structure was normal，the mitochondrial membrane potentialwas increased and the apoptosis rate was significantly reduced（P<0.05）. The mRNA expressions ofmTOR and tFAM in C2C12 cells weres significantly increased in the combination group（P<0.05），the expressions of FoxO1 protein in C2C12 cells in the FoxO1 inhibitor group，Liujunzi Decoction group and the combination group were significantly reduced，and the protein expressions of PGC-1α，FTO，mTOR and tFAM were significantly increased（P<0.05）. Compared with those in the Liujunzi Decoction group，the PGC-1αprotein expression in C2C12 cells in combination group significantly increased（P<0.05）.

Liujunzi Decoction can improve mitochondrial function and myoblast differentiation in tumor environment by interfering with PGC-1α/FoxO1 signal pathway.

To study the difference in odor substances between Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium）.

Electronic nose technology and gas chromatography-ionmobility spectroscopy（GC-IMS）were used to detectvolatile substances in the Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium）. The NIST and IMS databases were used to qualitatively and quantitatively analyze volatile substances. Plug-ins for the instrumentwas used to perform the dynamic principal component analysis of Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium）. The SIMCA-P 14.0 software was used to perform orthogonal partial least squares-discriminant analysis of volatile substances. The microbiotic platform was used to perform cluster heatmap analysis of volatile substances. The fingerprints and the characteristic component of Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium）sampleswere compared and analyzed. The volatile substances of students't-test with P<0.05 and orthogonal partial least squares-discriminantanalysis with VIP>1 were selected asmarkers of odor differences.

There were significant differences in the volatile components of Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium. Four characteristic substances were identified in Nanchaihu（B. Scorzonerifolium），namely decanal-M，linalool oxide，2，3-diethyl-5-methylpyrazine and 3-methyl-2-butenal-D. Two characteristic substances were identified in Beichaihu（Bupleurum Chinense），namely 3-methylbutanol-D and 2-methylpropanoic acid. A total of 24 markers of odor differences were screened，which could be used as the basis for distinguishing between Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium）.

The GC-IMS or electronic nose technology can quickly identify Beichaihu（Bupleurum Chinense）and Nanchaihu（B. Scorzonerifolium）. The gas of rancid oil of Nanchaihu（B. Scorzonerifolium）may be related to its characteristic substance 3-methyl-2-butenal-D and markers of odor differences such as decanal，（E）-2-hexenal，octanal，heptanal，hexanal，acrolein，isobutanal，et al.

Based on Fourier transform infrared（FTIR）spectroscopy，to establish a quantitativemodel for the rapid detection of the active ingredients of Beichaihu（Bupleurum chinense DC.），achieve the prediction of the contents of unknown active ingredients of Beichaihu（Bupleurum chinense DC.）and provide amethodological basis for the rapid and nondestructive detection of the active ingredients of Chinese herbalmedicines.

FTIR spectroscopy was used to collect the infrared spectra of 54 samples of Beichaihu（Bupleurum chinense DC.）from different harvesting periods，and high performance liquid chromatography（HPLC）was used to determine the contents of saikosaponins A（Ss-a），saikosaponins D（Ss-d），saikosaponins C（Ssc）and saikosaponins F（Ss-f）in 54 samples of Beichaihu（Bupleurum chinense DC.），and the contents of these saponinswere counted as Ss-a，Ss-d，Ss-c and Ss-f. The content of total saikosaponins（Ss-s）of Beichaihu（Bupleurum chinense DC.）was analyzed using TQ analysis software，and the spectra were processed by chemometric methods partial least squares（PLS），stepwisemultiple linear regression（SMLR），multiple scattering correction（MSC），standard normal transformations（SNT），firstorder derivatives（1stDer），second-order derivatives（2ndDer）and SG convolutional smoothing and Norris derivative filtering（ND）andmodeling.

SMLR+SNT+2ndDer+SG was the best for the content determination of Ss-c and Ss-f，with the correlation coefficients of 0.836 5 and 0.832 5，and themean absolute deviations of -0.01% and -0.01%，respectively and SMLR+MSC+2ndDer+SG was the best for the content determination of Ss-a，Ss-d and Ss-s，with the correlation coefficients of 0.820 6，0.835 6 and 0.840 5，respectively，and the average absolute deviations were-0.08%，-0.19% and -0.20%，respectively.

The combination of FTIR spectroscopy and HPLC can achieve the rapid determination of the active ingredients of Beichaihu（Bupleurum chinense DC.）. This study provides a new method for the content detection of Chinese herbalmedicines，which can promote the quality of Chinese herbalmedicines to be stable and controllable to a certain extent.

To study the preparation of Danggui（Angelicae Sinensis Radix）oilmicrocapsules，screening out the best preparation process and quality evaluation.

The microcapsules of volatile oil from Danggui（Angelicae Sinensis Radix）were prepared by complex coacervation method with gelatin and gum arabic solution as the materials. Tts quality evaluation was carried out through the appearance，microscopy，electronmicroscopy，particle size and other aspects.

When the concentration of gelatin solution was 3%，the concentration of arabic gum solution was 1%，the proportion of volatile oil to bladdermaterial was 30%，the pH value of complex coacervation reaction was 4.2，the temperature of bladder formation was 40℃，the stirring speed was 600 r·min^-1，adding curing agent（5 mL）and curing for 30 min，the encapsulation efficiency was the highest（85.62%）. The microcapsulesmade by the optimal technology had good quality.

The microcapsules of Danggui（Angelicae Sinensis Radix）oil prepared by the optimal process have uniform particle size，stable process and good entrapment efficiency.

To explore the effects of Qiju Dihuang Pills（杞菊地黄丸）on inflammatory responses inmice with diabetic retinopathy based on the rapid accelerated fibrosarcoma（Raf）/mitogen-activated extracellular signal-regulated kinase（MEKERK）signaling pathway.

Fifty male C57BL/6Jmice were randomly divided into the normal group，the model group，the Qiju Dihuang Pills low-dose，middle-dose and high-dose groups. The modelwas established by intraperitoneal injection of streptozotocin（STZ）. After 12 weeks of modeling，the normal group and themodel group were given saline by gavage once a day，and the Qiju Dihuang Pills Groupswere given the corresponding dose ofQiju Dihuang Pillsmedicinal solution（11 g·kg^-1，22 g·kg^-1 and 44 g·kg^-1）once a day for 8 consecutive weeks. The changes in blood glucose，water intake，urine output，food intake and bodymass were measured monthly. The imaging tests［fundus angiofluorescence（FFA），optical coherence tomography angiography（OCTA）and optical coherence tomography（OCT）］were performed to assess the retinopathy. HE staining was used to assess retinal histopathological changes and immunofluorescence（IF）to detect the expressions of interleukin-6（IL-6），interleukin-17（IL-17）and tumor necrosis factorα（TNF-α）. ELISA was used to detect the levels of IL-6，IL-17 and TNF-αin peripheral blood. Western Blotwas used to detect the expressions of Raf，p-Raf，MEK，p-MEK，ERK and p-ERK in retinal tissues.

Compared with those of the normalgroup，the mice in the model group had high blood sugar at random，obvious symptoms of polydipsia，polyuria，polyphagia and weight loss；the thicknesswas significantly thinner. The expressions of IL-6，IL-17，TNF-α，p-Raf，p-MEK and p-ERK were significantly increased（P<0.01）. Compared with those in the model group，themice in the Qiju Dihuang Pills groups had high random blood sugar，and the symptoms of polydipsia，polyuria，polyphagia and weight loss were significantly improved. The blood vessel density was low，the diameter of the vessel was thickened，and the degree of tortuosity was significantly reduced. The structure was loose，and the overall thickness was significantly reduced. The expressions of IL-6，IL-17，TNF-α，p-Raf，p-MEK and p-ERK were significantly reduced（P<0.01）.

Qiju Dihuang Pills can effectively protect the retina and delay the progression of diabetic retinopathy，which may be related to its regulation of Raf/MEK/ERK signaling pathway and its anti-inflammatory effect.

To study the consistency of the chemical and blood-entry components between Houpo Wenzhong Decoction（厚朴温中汤）and its dispensing granules，and to explore the applicability of the dispensing granules to replace the traditional decoction.

Lard and cold vinegar were used to create a ratmodel of deficiency-cold in spleen and stomach，and the drug efficacy was evaluated by biochemical indexes of rats in each administration group with ELISA kit. Ultra performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS）was used to study the chemical components，blood-entry prototypes and metabolites of Houpo Wenzhong Decoction and its dispensing granules. Compositional identification and characterisation were performed based on retention time，precise relative molecular mass，secondary mass spectrometry cleavage fragments and controls. The consistency of the chemical and blood-entry components of Houpo Wenzhong Decoction and its dispensing granules was evaluated by combining PeakView，Maker View and Metabolite Pilot software for pattern recognition.

A supervised orthogonal partial least squares（OPLS-DA）method with a setting of VIP>2.5 was used to screen the two for 66 differentiated chemical components，14 differentiated blood-entry prototype components and 12 differentiated blood-entry metabolites.

From the biochemical indexes，there was no significant difference between Houpo Wenzhong Decoction and its dispensing granules. From the chemical constituents，flavonoids and lignans，such as hesperidin and sanguinarine，were largely different. After entering the blood，the difference among some of the constituents was significantly reduced，especially the difference among flavonoids，such as hesperidin and sanguinarine，which seemed to have a“flattening effect”after the components entering the blood.

To explore the clinical effect and safety of Qijia Lifei Capsule（芪甲利肺胶囊）combined with entecavir in the treatment of primary positive pulmonary tuberculosis complicated with hepatitis B.

A total of 102 patients with newly treated bacteriopositive pulmonary tuberculosis combined with hepatitis B（admitted from January 2022 to November 2022）were included in this study. According to the random number table method，they were divided into observation group and control group，with 51 patients in each group，including 1 patient felling out in the control group and 2 patients felling out in the observation group. The control group（50 cases）were given entecavir treatment. The observation group（49 cases）were treated with entecavir combined with Qijia Lifei Capsule，and the data of each group［efficacy，changes of TCM symptom scores（afternoon tidal fever，shortness of breath and low voice，night sweats and spontaneous sweating，phlegm，cough，etc.），changes of inflammatory response indexes and immune indexes，liver function indexes and incidence of liver function damage and improvement rate of quality of life］was observed.

Compared with that in the observation group，the total effective rate of the control group was lower（P<0.05）. Before treatment，TCM syndrome scores（afternoon tidal fever，shortness of breath and low voice，night sweats and spontaneous sweating，phlegm，cough，etc.），the levels of tumor necrosis factor-α（TNF-α），C-reactive protein（CRP），white blood cell count（WBC），immunoglobulin A/G/M（IgA/IgG/IgM），serum total bilirubin（TBiL），aspartate aminotransferase（AST），glutamyl transpeptidase（GGT），alkaline phosphatase（ALP），alanine aminotransferase（ALT）and other liver function indexeswere not significant（P>0.05）. After treatment，TCM syndrome scores（afternoon tidal fever，shortness of breath and low voice，nightsweats and spontaneous sweating，phlegm，cough，etc.），the levels of TNF-α，CRP，WBC，IgA/IgG/IgM，TBiL，AST，GGT，ALP，ALT and other indexes in observation group were better than those in the control group（P<0.05）. Compared with that in the controlgroup，the incidence of liver function impairmentwas lower in the observation group after treatment，and the improvement rate of life quality was higher（P<0.05）.

Qijia Lifei Capsule combined with entecavir has significant clinical effect in the treatment of primary bacteriopositive pulmonary tuberculosis complicated with hepatitis B，which can better improve the condition，immune function and quality of life of the patients to a certain extent and reduce the liver damage，and the treatment is safe and reliable.

To observe the clinical effect and safety of Hushi Yiwei Decoction（胡氏益胃汤）combined with acupuncture in the treatment of chronic atrophic gastritis.

A total of 104 patients with chronic atrophic gastritis（admitted from January 2021 to June 2023）were included for group study. The patients were randomly divided into control group（Furazolidone Tablets and Cisaprid Tablets）and traditional Chinese medicine group（Furazolidone Tablets and Cisaprid Tablets，Hushi Yiwei Decoction and acupuncture）by random number table method，and 52 cases in each group. During the study，3 cases fell off in the traditional Chinese medicine group and 1 case fell off in the control group. The effect，the changes of TCM syndrome scores（epigastric fullness，stomachache and distention）and gastroscopy indexes（degree of enterization，gastric mucosa characteristics and degree of gastric atrophy）before and after treatment，the levels of serum gastrointestinal hormone indexes（gastrin and somatostatin gastrin），the levels of serum inflammatory factors［interleukin-6（IL-6），interleukin-8（IL-8），tumour necrosis factor-α（TNF-α）］，the positive rates of Helicobacter pylori（HP）before and after treatment，the levels of T lymphocytes（CD₄⁺，CD₈⁺，CD₄⁺/CD₈⁺）and adverse reactions were observed.

The treatment effect of the traditional Chinese medicine group was better than that of the control group（P<0.05）. Before treatment，TCM syndrome scores（gastric epigastric fullness and acid belching，abdominal pain and distension），gastroscopy indexes（degree of enterization，gastric mucosa characteristics and degree of gastric atrophy），serum gastrointestinal hormone indexes（motilin，somatostatin and gastrin），serum inflammatory factor levels（IL-6，IL-8 and TNF-α），HP positive rate and immune function indexes（CD₄⁺，CD₈⁺，CD₄⁺/CD₈⁺）of all patients in each group were not significant（P>0.05）. After treatment，TCM syndrome scores（gastric epigastric fullness，acid belching，abdominal pain and distention），gastroscopy indexes（degree of enterification，gastricmucosa characteristics and degree of gastric atrophy），gastrin levels，IL-6，IL-8 and TNF-αlevels，HP positive rate and CD₈⁺ indexes were all decreased in both groups. The levels of motilin and somatostatin，CD₄⁺ and CD₄⁺/CD₈⁺ were increased，and the increase and decrease of all indexes in the traditional Chinese medicine group after treatment were greater than those in the control group（P<0.05）. The adverse reaction rate of traditional Chinese medicine group and control group was lower（P>0.05）.

Hushi Yiwei Decoction combined with acupuncture therapy has significant clinical effect in the treatment of chronic atrophic gastritis，which can effectively relieve the symptoms，alleviate the condition and improve the gastrointestinal function and immune function of patients，reduce the positive rate of HP and the adverse reaction，and is safe and reliable.

To explore the effects of electroacupuncture therapy on intestinal flora and inflammatory immune function in ischemic stroke model rats.

According to random number table method，16 Sprague Dawley（SD）rats were divided into sham operation group，middle cerebral artery occlusion（MCAO）group，MCAO+electroacupuncture group and MCAO+electroacupuncture non-acupoint group. Left MCAO operation was conducted with wire occlusion method to prepare brain ischemia-reperfusion animalmodel. After the operation，the animal model was put into wood shavings and kept warm water to observe the changes. After 2 hours，the wire occlusion was slowly withdrawn，while only the arteries were separated and then sewed up in the sham operation group.24 hours aftermodeling，the MCAO+electroacupuncture group selected Shenting（DU24）and Baihui（DU20）and applied SDZ-V electroacupuncture instrument with density wave（1～20 Hz）once a day，for a total of 7 days. The sham operation group and the MCAO group were given the same feeding and grasping conditions with out any treatment. In the MCAO+electroacupuncture non-acupoint group，the hypocholic non-meridian and non-acupoint pointswere selected，and the electroacupuncture intervention was carried out24 hours aftermodeling for 14 days. The neural function of rats in each group was observed 24 h and 3，7 and 14 days after themodeling，and the change of brain water contentwas detected after the intervention for 14 days. After successfulmodeling，longamethod was used to score the neurological impairment. Morriswatermaze experiment evaluated the spatial learning and memory ability of rats. Western blotanalysiswas performed to detect the growth related proteins of ischemic penumbra neurons in each group. Real-time fluorescence quantitative Polymerase Chain Reaction（PCR）was used to detect the changes of intestinal flora in rat stool. The contents of interleukin-1β（IL-1β）and intercellular adhesionmolecule-1（ICAM-1）in serum and brain tissue were detected by double antibody sandwich ABC-enzyme-linked immunosorbent assay（ABC-ELISA）. The correlation between IL-1β，ICAM-1 and intestinal flora was analyzed.

The neurological function of the rats in each group was evaluated at 24 h and 3，7，14 d aftermodeling. The neurological function deficit score of the rats in the electroacupuncture group was lower than that in the MCAO group at the same period after cerebral ischemia reperfusion，and the acupoint group was significantly lower than that in the non-acupoint group（P<0.01）. The improvement of nerve function in electroacupuncture group wasmore significant with the extension of intervention time. Compared with thatof the MCAO group，the escape latency ofMCAO+electroacupuncture group was significantly shortened（P<0.05），and the number of crossing platform in the MCAO+electroacupuncture group was increased（P<0.05）. Compared with those of the MCAO group，the expressions of growth associated protein-43（GAP-43）and neurofilament-200（NF-200）increased（P<0.05），and the expressions of repulsive guidancemolecule a（RGMa）decreased（P<0.05）in the electroacupuncture group and non-acupoint group. The water content of brain tissue in electroacupuncture group decreased significantly（P<0.01）. Compared with that of the MCAO group，the number of Escherichia coli and enterococcus in feces of the MCAO+electroacupuncture group decreased（P<0.05），while the number of bifidobacterium and lactobacillus increased（P<0.05）. The levels of IL-1β and ICAM-1 in peripheral blood and brain tissue of ischemic stroke rats in the electroacupuncture group were decreased（P<0.05）. IL-1βand ICAM-1 were negatively correlated with the number of lactobacillus and bifidobacterium，butpositively correlated with the number of Escherichia coliand enterococcus.

Electroacupuncture can regulate intestinal flora disturbance in ischemic stroke ratmodel，reduce inflammatory response through anti-inflammatory and maintenance of immune tolerancemechanism，and play a role in brain protection.

To observe the clinical effect of Maxing Shigan Decoction（麻杏石甘汤）combined with amoxicillin sodium and clavulanate potassium in the treatment of children with pulmonary infection and its influence on inflammation and other indicators.

A total of 85 children with pulmonary infection were included in the study，all of whom were admitted to the Department of Pediatrics of the hospital from December 2020 to February 2023，meeting the diagnosis of pulmonary infection. The children were divided into control group（42 cases）and observation group（43 cases）by random number table method，and the control group was treated with amoxicillin sodium and clavulanate potassium. The children in the observation group were treated with amoxicillin sodium clavulanate potassium combined with Maxing Shigan Decoction. During the treatment，1 case fell off in the control group and 2 cases fell off in the observation group. The data of children in each group was observed，including treatment effect，fever disappearance time，lung infection absorption time，cough relief time，heart rate respiratory recovery time and the time of disappearance of lung rales，the scores of traditional Chinese medicine symptoms（cough，asthma，expectoration，fever，irritability，etc.）changes before and after treatment，changes of inflammatory factor indicators and lung function indicators，changes of oxygenation index and lung infection score，length of hospital stay and adverse reactions.

The treatmenteffectof the observation group was better than that of control group（P<0.05）. Compared with those of the control group，the disappearance time of fever，absorption time of pulmonary infection，cough relief time，heart rate and respiration recovery time and the disappearance time of lung rales in observation group were shorter（P<0.05）. The levels of tumor necrosis factor-α（TNF-α），interleukin 6（IL-6），interleukin 8（IL-8）and other inflammatory markers，the scores of TCM symptoms（cough，asthma，phlegm，fever，irritability，etc.）in each group before treatment，forced vital capacity，FVC，forced expiratory volume in one second（FEV1）and other pulmonary function indexes，oxygenation index and pulmonary infection score were not significant（P>0.05）. After treatment，the scores of TCM symptoms（cough，asthma，sputum，fever，irritability，etc.），inflammatory factor indexes（TNF-α，IL-6 and IL-8），pulmonary function indexes（FVC，FEV1），oxygenation index，pulmonary infection score and other indexes were improved in both groups. Those in the observation group were all better than those in the control group after treatment（P<0.05）. The hospitalization time of the observation group was shorter than that of the control group and the hospitalization cost was less than that of control group（P<0.05）. The adverse reaction rate was lower in both groups（P>0.05）.

The therapeutic effect of Maxing Shigan Decoction combined with amoxicillin sodium and clavulanate potassium in the treatment of children with pulmonary infection is accurate. The symptoms of children are improved，with fast recovery and improved lung function. There are fess adverse reactions. The treatment is safe and reliable，and it is worthy of application.

To explore the efficacy of Yishen Tiaogan Decoction（益肾调肝方）on perimenopausal women with dry eye of kidney deficiency and liver depression syndrome and its influence on early changes in ocular surface.

One hundred and twenty perimenopausal women with dry eye of kidney deficiency and liver depression syndrome admitted to the hospital from February 2021 to March 2023 were selected，and randomly assigned into two groups，each with 60 cases. The control group received sodium hyaluronate eye drops. Based on this，the study group was given Yishen Tiaogan Decoction. The treatment in both groups lasted for 4 weeks. Then the TCM symptom scores，ocular surface function［tear film break-up time（BUT），Schirmer I test（SIT），cornea corneal fluorescein（FL）staining］，clinical efficacy，the levels of inflammatory factors［interleukin（IL）-6，IL-33］and adverse reactions were compared between two groups after treatment.

TCM syndrome scores showed a decline in both groups after treatment（P<0.05），and the score was（1.60±0.35）point in the study group，which was lower than that［（4.89±0.74）point］in the control group（P<0.05）. Therewas an improvement in BUT，SIT and FL score in both groups after treatment（P<0.05），and the three parameters were（10.08±1.29）s，（11.25±2.26）mm/5 min and（0.85±0.14）point in the study group，which were all better than those［（5.64±1.15）s，（7.76±1.35）mm/5 min and（2.38±0.49）point］in the control group，with statistical difference（P<0.05）. In the control group，15 cases were cured，24 cases were improved and 21 cases were ineffective. In the study group，35 cases were cured，22 cases were improved and 3 cases were ineffective. The efficacy grade distribution yielded statistical difference between two groups（Z＝-5.044，P<0.05）. The overall efficacy rate was 95.00%（57/60）in the study group，which was higher than that（65.00%，39/60）in the control group（P<0.05）. Serum levels of inflammatory factors showed a decrease in both groups after treatment（P<0.05）. The serum levels of IL-6 and IL-33 were（15.13±3.30）pg/mL and（10.30±1.85）pg/m L in the study group，which were lower than those［（17.26±3.71）pg/mL and（11.49±2.18）pg/mL］in the controlgroup（P<0.05）. During the treatment period，no adverse reactions were found in the control group，and one case of diarrhoea occurred in the study group，the symptoms were mild and could be relieved without any intervention. The adverse reaction rate yielded no statistical difference between two groups（χ²＝1.008，P>0.05）.

Yishen Tiaogan Decoction for perimenopausal women with dry eye of kidney deficiency and liver depression syndrome can effectively alleviate the clinical symptoms，improve ocular surface function and attenuate inflammation response with no significant adverse reactions，which is worthy of recommendation.

To evaluate the regulatory effectof Tiaozhong Xiaqi Decoction（调中下气汤）combined with gastricmucosa protectant on serum gastrin in patients with chronic atrophic gastritis.

A total of 112 patients with chronic atrophic gastritis who came to the hospitalwere selected as the study subjects. According to the randomized control method，the invalids were divided into two groups. The reference group（56 cases）was treated with conventional Western medicine，orally taking Weifuchun Tablets（胃复春片），4 tablets per time，3 times per day. The Helicobacter pylori（HP）positive individuals were treated with HP eradication regimen and quadruple therapy for 10 days. On the basis of treatment in the reference group，the study group（56 cases）received Tiaozhong Xiaqi Decoction orally. Both groups continued the treatment for 3 months. The changes in traditional Chinese medicine syndrome scores，including cold pain in the stomach，bloating in the stomach，liking warmth and pressing，vomiting with clear water，shortness of breath，lazy speech and loose stools before and after treatment between two groups were compared. The changes in pathological scores under gastroscopy between the two groups before and after treatment were compared，and the changes in serum levels of gastrin（GAS），somatostatin（SS）and epidermal growth factor（EGF）and treatment efficacy before and after treatment were compared.

The treatment efficacy of the study group was better than thatof the reference group，and the difference was statistically obvious（P<0.05）. After treatment，the scores of spleen and stomach deficiency cold syndrome related symptoms in both groups showed a decrease，and the reduction in scores of spleen and stomach deficiency cold syndrome related symptoms in the study group was better than that in the reference group（P<0.05）. After treatment，both groups showed a decrease in pathological scores under gastroscopy，and the reduction in pathological scores under gastroscopy in the the study group was better than that in the control group（P<0.05）. After treatment，the levels of GAS in both groups decreased，while the levels of SSand EGF increased. Among them，the study group had a better decrease in serum GAS than the reference group，while the increase in SSand EGFwas better than that in the reference group（P<0.05）.

Tiaozhong Xiaqi Decoction combined with gastric mucosa protector is effective in the treatment of chronic atrophic gastritis. It can reduce the TCM symptom scores of patients，alleviate the atrophic state of gastric mucosa，regulate gastrointestinal hormones，inhibit the excessive secretion of gastrin，protect gastric mucosa tissue，accelerate the recovery of gastric function and improve the treatment effect.

To analyze the effectof Xuefu Zhuyu Decoction（血府逐瘀汤）on lipoprotein-associated phospholipase A2（Lp-PLA2），monocyte chemoattractant protein-1（MCP-1），soluble intercellular adhesion molecule-1（sICAM-1），inflammatory factors and quality of life in patients with coronary heart disease unstable angina pectoris with phlegm turbidity and obstruction type.

A total of 120 patients with coronary heart disease unstable angina pectoris with phlegm turbidity and obstruction were included，and they were randomly divided into observation group and control group，60 cases in each group. The patients in the control group were treated with conventional western medicine，and the observation group was given Modified Xuefu Zhuyu Decoction on this basis，and the treatment period of both groups was 8 weeks. After the treatment，the clinical curative effect，angina pectoris attack，TCM clinical symptom scores，inflammatory factor levels，Lp-PLA2，MCP-1，sICAM-1 and quality of life were observed in the two groups.

After treatment，the total effective rate of the observation group was 90.00%（54/60），which was significantly higher than that of the control group（P<0.05）. At the same time，the frequency of angina pectoris attack，duration and pain degree of the observation group were more significantly improved than those of the control group（P<0.05）. The changes of TCM clinical symptom scores of body fatigue，excessive phlegm and anorexia were significantly improved compared with those before treatment（P<0.05）and the improvement of each symptom score in the observation group was significantly better than that in the control group（P<0.05）. The levels of interleukin-4（IL-4），interleukin-6（IL-6），interleukin-8（IL-8）and tumor necrosis factor-α（TNF-α）of the two groups after treatment decreased compared with those before treatment（P<0.05）and the decrease in the observation group was better than that of the control group. The levels of Lp-PLA2，MCP-1 and sLCAM in the two groups after treatment were significantly lower than those before treatment（P<0.05），and the improvement in the observation group was significantly better than that in the control group（P<0.05）. After treatment，the MOS item short from health survey（SF-36）scores of the two groups were both significantly improved compared with those before treatment（P<0.05），and the quality of life in the observation group was significantly better than that in the control group.

Xuefu Zhuyu Decoction can effectively improve the clinical treatment effect of patients with coronary heart disease unstable angina pectoris with phlegm turbidity and obstruction type，reduce the frequency and degree of angina pectoris，improve the clinical symptoms of patients，increase the levels of inflammatory factors，reduce the levels of Lp-PLA2，MCP-1 and sICAM-1 and improve the quality of life of patients.

To explore the effectof Chaihu Qingshang Decoction（柴胡清上汤）combined with gaze stability vestibular rehabilitation training on vestibular function and oxidative stress in patients with vestibular neuritis.

Sixty patients with vestibular neuritis who were diagnosed in the hospital from July 2021 to December 2022 were selected as research objects，and were randomly divided into the experimentgroup and the control group，with 30 patients in each group. The control group was given routine and symptomatic treatment，and the experiment group was given Chaihu Qingshang Decoction combined with gaze stability vestibular rehabilitation training on the basis of the control group. The clinical efficacy，dizziness symptom assessment scale，dizziness disorder scale，specific activity balance confidence scale，vestibular function，serum inflammatory factors，oxidative stress indicators and incidence of adverse reactions were compared between the two groups before and after treatment.

After treatment，compared with the total effective rate（63.33%，19/30）in the control group，the total effective rate（86.67%，26/30）in the experimental group was higher（P<0.05）. Compared with those before treatment，the Vertigo Symptom Rating Scale（DARS）and Dizziness Handicap Inventory（DHI）scores of both groups were significantly reduced，while the activities-specific balance confidence scale（ABC）scores were increased（P<0.05）. After treatment，compared with the control group，the experimental group had lower DARSand DHIscores，and higher ABC scores（P<0.05）. Compared with that before treatment，semicircular canal paresis（CP）value，scanning wave amplitude，spontaneous nystagmus intensity and shaking head nystagmus intensity of semicircular canals paresis patients in the two groups decreased significantly，and semicircular canals gain increased significantly（P<0.05）. After treatment，compared with those of the control group，the CP value，scanningwave amplitude，spontaneous nystagmus intensity and shaking head nystagmus intensity of semicircular canals paresis in the experiment group were lower，and the semicircular canals gain was higher（P<0.05）. Compared with those before treatment，the serum levels of IL-6 and TNF-αin both groups significantly decreased（P<0.05）. And compared with those of the control group，the levels of the experimental group were relatively low（P<0.05）. Compared with those before treatment，the levels of SOD in both groups significantly increased，while the levels of MDA decreased（P<0.05）. Compared with the control group，the experimental group had higher level of SOD and lower level of MDA（P<0.05）. There was no statistically significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Chaihu Qingshang Decoction combined with gaze stability vestibular rehabilitation training can effectively improve the overall therapeutic effect of patients with vestibular neuritis，regulate inflammation and oxidative stress in the body，significantly improve vestibular function，alleviate nystagmus and semicircular canals paresis，which is suitable for clinical promotion.

Ultra performance liquid chromatography coupled with quadrupole/electrostatic field orbital trap high resolution mass spectrometry（UHPLC-Q Exactive Focus MS/MS）method was developed to rapidly analyze and identify the alkaloids from Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）.

The chromatographic separation was performed on the Waters Acquity UPLCBEH C₁₈ column（2.1 mm×50 mm，1.7 μm）with acetonitrile-0.1% formic acid water solution as themobile phase for gradient elution. The electrospray ion source（ESI）was adopted to collect the data in positive ionization modes，and the detection range was m/z 100～1500.

The chemical constituents were identified and confirmed by analyzing themass data from the reference substances and literature. A total of 228 alkaloidswere identified，including 21 C₁₈ alkaloids，145 C₁₉ alkaloids［42 diester diterpenoid alkaloids（DDAs），47 monoester diterpenoid alkaloids（MDAs），42 aminoalcohol-diterpenoid alkaloids（ADAs），14 lipo alkaloids（Lipo-As）］，27 C₂₀ alkaloids and 35 others，28 of them were discovered for the first time.

The establishedmethod systematically analyzed the alkaloids of Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）. It provides a reference for the subsequent study ofmaterial basis and developmentof Shaanxiproduced crude Fuzi（Aconiti Lateralis Radix Praeparata）.

To study the acute toxicity and liver and kidney toxicity of raw Zhizi（Gardeniae Fructus）and six water extractswith different processing degrees using zebrafish model and explore the effect of processing degree on Zhizi（Gardeniae Fructus）toxicity.

Acute toxicity studieswere carried outon AB series zebrafish with normal development 3 days（3 dpf）after fertilization，and 6 different dose concentrationswere set for raw products（sp）and sixwater extractswith different degrees of preparation（pz1，pz2，pz3，pz4，pz5，pz6）. Themortality rate of zebrafish at72 h was statistically calculated. Quantitation-toxicity curveswere created using GraphPad Prism 9.0 software to calculate half lethal concentration（LC₅₀）and 10% lethal concentration（LC₁₀）. Liver and kidney injury studies were conducted on gz15Tg/+（AB）liver fluorescent protein transgenic zebrafish with normal development4 days（4 dpf）after fertilization. Three dose concentration groups were set up as low（LC₀），medium（LC₁₀）and high（LC₂₀）groups，and the acetaminophen group was setup，with embryonic water as the blank group. After72 h of administration，zebrafish livermorphology and fluorescence area changes were observed，and the activities of alanine aminotransferase（ALT）and aspartate aminotransferase（AST），creatinine（Cr）and urea nitrogen（BUN）were detected.

The results of acute toxicity experiments showed that the values of LC₅₀ of the raw productand six water extractswith different processing degreeswere 0.042，0.049，0.053，0.064，0.050，0.058 g·L^-1 respectively. The values of LC₁₀ were 0.024，0.025，0.033，0.038，0.036，0.036，0.039 g·L^-1 respectively. The results of liver and kidney injury showed that compared with those in the blank group，the livermorphological deformation and fluorescence area of zebrafish in the acetaminophen group decreased（P<0.01）and the activities of ALT and AST increased（P<0.01），indicating that acetaminophen was hepatotoxic to zebrafish. The livermorphological changes and fluorescence area of zebrafish in the low，medium and high groups of sp decreased（P<0.01），the activities of ALT and AST increased（P<0.01，P<0.05）and the activities of BUN and Cr rised（P<0.05），indicating the raw product had hepatic and renal toxicity to zebrafish. Livermorphology and fluorescence area of zebrafish decreased in themedium and high dose groups of pz 1，pz 2 and pz 3（P<0.01），the activities of ALT and AST in pz1 group increased（P<0.01），the activities of BUN and Cr elevated（P<0.01，P<0.05），the activity of ALT in the low，medium and high dose groups of pz2 and pz3 rised（P<0.05，P<0.01），and the activities of AST in themedium and high dose groups increased（P<0.05，P<0.01）. There were no significant changes of livermorphology or fluorescence area in the low and medium dose groups of pz4，pz5 and pz6，and there were changes in the high dose group（P<0.05）. The results showed that the low and medium dose groups of pz4，pz5 and pz6 had no significant effect on the liver of zebrafish，and there was liver damage in the high dose group. ALT and AST were elevated in themedium and high dose groups of pz4（P<0.05，P<0.01）. There was no significant effect on the decrease in the activity of ALT of pz5 and pz6 and there was liver injury in the AST high dose group（P<0.01，P<0.05）. The activities of BUN and Cr were increased in the high dose groups of pz3 and pz5（P<0.01，P<0.05），and the activitieswere elevated in the high dose group of pz4（P<0.01）. The results showed that sp and pz1～pz5 had different degrees of damage to the liver and kidney of zebrafish with different degrees of preparation and different doses. There was no liver or kidney injury neither in the three dose concentration groups of pz6 nor in the ALT，BUN，Cr and AST low and medium dose groups of zebrafish.

The liver and kidney injury of Zhizi（Gardeniae Fructus）is related to the degree of processing and dosage. But raw and different processing degrees products showed certain liver and kidney damage to varying degrees. Standardizing the processing conditionsmay be an importantmeasure to realize themedication safety of gardenia.