Home Journals Articles Updates About
CN
image
收藏切换
Rapid Identification of Alkaloids Components in Shaanxi-produced Crude Fuzi（Aconiti Lateralis Radix Praeparata）Based on UHPLC-Q-Exactive Focus-MS/MS Technique
收藏切换
Shasha LI1, Fang LI1, Jingxia ZHNAG2, Fan LI1, Ajuan Mao1, Yajuan LI3, Xi CHEN4, Xin HAN4, Weifeng WANG1
Chinese Archives of Traditional Chinese Medicine | 2025, 43(1) : 231 - 240
Less
收藏切换
Chinese Archives of Traditional Chinese Medicine | 2025, 43(1): 231-240
• Platform of Provincial Project •
Rapid Identification of Alkaloids Components in Shaanxi-produced Crude Fuzi（Aconiti Lateralis Radix Praeparata）Based on UHPLC-Q-Exactive Focus-MS/MS Technique
Full
Shasha LI1, Fang LI1, Jingxia ZHNAG2, Fan LI1, Ajuan Mao1, Yajuan LI3, Xi CHEN4, Xin HAN4, Weifeng WANG1
Affiliations
  • 1.［Shaanxi Provincial Traditional Chinese Medicine Research Institute, Xi’an 710003, Shaanxi, China
  • 2.Xi’an International Medical Center, Xi’an 710119, Shaanxi, China
  • 3.Shulan（Anji）Hospital, Huzhou 313399, Zhejiang, China
  • 4.Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi, China］
Published: 2025-01-10 doi: 10.13193/j.issn.1673-7717.2025.01.042
Outline
收藏切换
Objective

Ultra performance liquid chromatography coupled with quadrupole/electrostatic field orbital trap high resolution mass spectrometry（UHPLC-Q Exactive Focus MS/MS）method was developed to rapidly analyze and identify the alkaloids from Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）.

Method

The chromatographic separation was performed on the Waters Acquity UPLCBEH C18 column（2.1 mm×50 mm，1.7 μm）with acetonitrile-0.1% formic acid water solution as themobile phase for gradient elution. The electrospray ion source（ESI）was adopted to collect the data in positive ionization modes，and the detection range was m/z 100～1500.

Results

The chemical constituents were identified and confirmed by analyzing themass data from the reference substances and literature. A total of 228 alkaloidswere identified，including 21 C18 alkaloids，145 C19 alkaloids［42 diester diterpenoid alkaloids（DDAs），47 monoester diterpenoid alkaloids（MDAs），42 aminoalcohol-diterpenoid alkaloids（ADAs），14 lipo alkaloids（Lipo-As）］，27 C20 alkaloids and 35 others，28 of them were discovered for the first time.

Conclusion

The establishedmethod systematically analyzed the alkaloids of Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）. It provides a reference for the subsequent study ofmaterial basis and developmentof Shaanxiproduced crude Fuzi（Aconiti Lateralis Radix Praeparata）.

UHPLC-Q Exactive Focus MS/MS  /  Shaanxi-produced crude Fuzi（Aconiti Lateralis Radix Praeparata）  /  alkaloids  /  component analysis
李莎莎, 李芳, 张景霞, 李凡, 毛阿娟, 李雅娟, 陈曦, 韩昕, 王卫锋. 基于UHPLC-Q-Exactive Focus-MS/MS技术对陕产附子中生物碱类成分的快速辨识. 中华中医药学刊, 2025 , 43 (1) : 231 -240 . DOI: 10.13193/j.issn.1673-7717.2025.01.042
Shasha LI, Fang LI, Jingxia ZHNAG, Fan LI, Ajuan Mao, Yajuan LI, Xi CHEN, Xin HAN, Weifeng WANG. Rapid Identification of Alkaloids Components in Shaanxi-produced Crude Fuzi（Aconiti Lateralis Radix Praeparata）Based on UHPLC-Q-Exactive Focus-MS/MS Technique[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (1) : 231 -240 . DOI: 10.13193/j.issn.1673-7717.2025.01.042
Appendix
Less
Year 2025 volume 43 Issue 1
43
16
Cite this Article
BibTeX
Article Info
doi: 10.13193/j.issn.1673-7717.2025.01.042
  • Online Date：2026-10-30
  • Published：2025-01-10
Article Data
Affiliations
History
Funding
Affiliations
    1.［Shaanxi Provincial Traditional Chinese Medicine Research Institute, Xi’an 710003, Shaanxi, China
    2.Xi’an International Medical Center, Xi’an 710119, Shaanxi, China
    3.Shulan（Anji）Hospital, Huzhou 313399, Zhejiang, China
    4.Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi, China］
References
Share
https://castjournals.cast.org.cn/joweb/zhzyyxk/EN/10.13193/j.issn.1673-7717.2025.01.042
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House