Full
Outline
To study the consistency of the chemical and blood-entry components between Houpo Wenzhong Decoction（厚朴温中汤）and its dispensing granules，and to explore the applicability of the dispensing granules to replace the traditional decoction.
Lard and cold vinegar were used to create a ratmodel of deficiency-cold in spleen and stomach，and the drug efficacy was evaluated by biochemical indexes of rats in each administration group with ELISA kit. Ultra performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS）was used to study the chemical components，blood-entry prototypes and metabolites of Houpo Wenzhong Decoction and its dispensing granules. Compositional identification and characterisation were performed based on retention time，precise relative molecular mass，secondary mass spectrometry cleavage fragments and controls. The consistency of the chemical and blood-entry components of Houpo Wenzhong Decoction and its dispensing granules was evaluated by combining PeakView，Maker View and Metabolite Pilot software for pattern recognition.
A supervised orthogonal partial least squares（OPLS-DA）method with a setting of VIP>2.5 was used to screen the two for 66 differentiated chemical components，14 differentiated blood-entry prototype components and 12 differentiated blood-entry metabolites.
From the biochemical indexes，there was no significant difference between Houpo Wenzhong Decoction and its dispensing granules. From the chemical constituents，flavonoids and lignans，such as hesperidin and sanguinarine，were largely different. After entering the blood，the difference among some of the constituents was significantly reduced，especially the difference among flavonoids，such as hesperidin and sanguinarine，which seemed to have a“flattening effect”after the components entering the blood.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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