Through in vitro studies，it explored whether Supplemented Buyang Huanwu Decoction（加味补阳还五汤，SBHD）could promote adipose stem cells（ADSCs）in greater omentum to paracrinehepatocyte growth factor（HGF）and whether this effect could inhibit the epithelial-mesenchymal transition（EMT）of peritonealmesothelial cells（PMC）in order to initially clarify the pharmacological mechanism and target of this prescription in preventing and treating postoperative abdominal adhesion（PAA）.

Experiment 1 clarified whether the inhibitory effect of SBHD on the EMT of PMCwas a directeffectof the formula on PMC or an indirect effect by regulating the paracrine secretion of ADSCs in the greater omentum. After culturing ADSCs with SBHD，conditional culture media（hereinafter referred to as“Chinese medicine culture media”）were prepared to intervene in the PMC of EMTmodels，and the expression levels of EMT related proteins E-cadherin（E-Cad）andα-smooth muscle actin（α-SMA）were detected by Western Blot. Experiment 2 clarified whether the inhibitory effectof SBHD on EMT of PMC was related to the secretion of HGF by ADSCs in the greater omentum. The intervention of PMC used three types ofmedia：low HGF expressing ADSCs conditioned medium（hereinafter referred to as “low HGFmedium”），normal ADSCs conditioned medium（hereinafter referred to as“normal medium”）and traditional Chinese medicine culture medium and the following indicators were detected.（1）ELISA was used to detect the content of HGF in the three media before and after intervention in PMC.（2）Western Blot，qPCR and IFmethods were used to detect the expression levels of EMT related proteins in each group of PMCs after intervention，and scratch assay was used to detect the migration ability of PMCs in each group.

Experiment 1：Effect of SBHD and traditional Chinese medicine culture medium on EMT related indicators：Compared with the model group，the SBHD group up-regulated the protein expression of E-Cad（P<0.05）and down-regulated the protein expression of α-SMA（P<0.05）. The traditional Chinese medicine culture medium group significantly up-regulated the protein expression of E-Cad（P<0.01）and significantly down-regulated the protein expression of α-SMA（P<0.01）. Compared with the SBHD group，the traditional Chinese medicine culture medium group up-regulated the protein expression of E-Cad（P<0.05）and significantly downregulated the protein expression of α-SMA（P<0.01）. Experiment 2：（1）Comparison of HGF contents in three different culture media before and after acting on PMC：Before acting on PMC，compared with the normal medium，the content in low HGF medium was significantly reduced（P<0.01），while the content in traditional Chinese medicine culture medium was significantly increased（P<0.01）. After acting on PMC，the content of each culture medium group significantly decreased.（2）Expression of EMT-related proteins：Based on the results of Western Blot，qPCR and IF，compared with the model group，all culture medium groups were able to increase E-Cad expression（P<0.05 or P<0.01）and decrease the expressions of α-SMA and vimentin（VIM）（P<0.05 or P<0.01）. All culture medium groups were able to inhibit the EMT of PMC，with the order of effect being Chinese medicine culture medium group>normal medium group>low HGF medium group.（3）Changes in cellmigration ability of EMT：After intervention for 24 h and 48 h，the healing rate of each group was significantly decreased compared with that of the model group（P<0.01）. Each group of culturemedia can reduce the Rmigration ability of the EMT model，with the order of effect being Chinese medicine culture medium group>normal medium group>low HGF medium group.（4）Correlation analysis of EMT-related protein expression and HGF content：The expression level of E-Cad protein was positively correlated with the content of HGF（P<0.05）. The protein expressions of α-SMA and VIM were negatively correlated with the content of HGF（P<0.05 or P<0.01）.

The therapeutic effect of SBHD in inhibiting the EMT of PMC and preventing PAA may be multi-target，with both direct effects on PMC and indirect effects by enhancing the paracrine secretion of HGF by ADSCs in the greateromentum，with the latter beingmore important.