To study the difference in odor substances between Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium).
Electronic nose technology and gas chromatography-ionmobility spectroscopy(GC-IMS)were used to detectvolatile substances in the Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium). The NIST and IMS databases were used to qualitatively and quantitatively analyze volatile substances. Plug-ins for the instrumentwas used to perform the dynamic principal component analysis of Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium). The SIMCA-P 14.0 software was used to perform orthogonal partial least squares-discriminant analysis of volatile substances. The microbiotic platform was used to perform cluster heatmap analysis of volatile substances. The fingerprints and the characteristic component of Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium)sampleswere compared and analyzed. The volatile substances of students't-test with P<0.05 and orthogonal partial least squares-discriminantanalysis with VIP>1 were selected asmarkers of odor differences.
There were significant differences in the volatile components of Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium. Four characteristic substances were identified in Nanchaihu(B. Scorzonerifolium),namely decanal-M,linalool oxide,2,3-diethyl-5-methylpyrazine and 3-methyl-2-butenal-D. Two characteristic substances were identified in Beichaihu(Bupleurum Chinense),namely 3-methylbutanol-D and 2-methylpropanoic acid. A total of 24 markers of odor differences were screened,which could be used as the basis for distinguishing between Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium).
The GC-IMS or electronic nose technology can quickly identify Beichaihu(Bupleurum Chinense)and Nanchaihu(B. Scorzonerifolium). The gas of rancid oil of Nanchaihu(B. Scorzonerifolium)may be related to its characteristic substance 3-methyl-2-butenal-D and markers of odor differences such as decanal,(E)-2-hexenal,octanal,heptanal,hexanal,acrolein,isobutanal,et al.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |