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Outline
To investigate the mechanism of berberine on energy metabolism and related pathways in EC9706 cells.
The cellswere routinely cultured，MTT screened，and two mass concentrations of 15 μg/mL and 30 μg/mL were selected. The cell cycle and apoptosis were detected by flow cytometry，the glycolytic potential and mitochondrial respiratory capacity were detected by energy metabolism analysis system，and the changes in the expression of phosphorylated adenylate activated protein kinase（p-AMPK）/adenylate activated protein kinase（AMPK）and the downstream phosphor ylated mammalian target of rapamyc in（p-mTOR）/mammalian target of rapamycin（mTOR）proteins were explored by Western Blotting. The expression levels of AMPK and downstream mTOR were verified at the gene level by quantitative real-time PCR（qPCR）.
Berberine significantly inhibited the proliferation of EC9706 cells（P<0.01）and promoted the apoptosis of EC9706 cells（P<0.05）. Compared with the control group，berberine and drug group showed a significant decrease in the G0/G1 phase（P<0.05），and a significant increase in the proportion of Sphase（P<0.01）. Berberine inhibited the glycolytic potentia and mitochondrial respiration of EC9706 cells（P<0.01）in a concentration-dependent manner，and berberine promoted p-AMPK/AMPK（P<0.01）and inhibited the expression of p-mTOR/mTOR protein（P<0.05）. At the gene level，berberine promoted AMPK expression（P<0.05）as well asmTOR expression（P<0.01）.
Berberine can inhibit the proliferation of oesophageal cancer EC9706 cells through energy metabolic pathway，promote apoptosis of oesophageal cancer cells and hinder their division.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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