The traditional single-cycle pulse recognition methods encounter significant limitations in practical applications due to the morphological diversity，cycle variability and individual differences inherent in the wrist pulse signals.In this study，it focused on solving the classification problem of multi-period pulse recognition，and constructed an image-based dataset by integrating the amplitude and temporal information of pulse signal through the Markov Transition Field（MTF）.Building upon this foundation，it set up new deep-learning network model，TCANet（Temporal Convolutional Attention Network），which leveraged the depth-wise separable convolutions and self-attention mechanisms to markedly improve the accuracy and efficiency of pulse image classification.The experimental outcomes revealed that TCANet achieved the impressive performance metrics，including 94.74% accuracy，94.79% precision，94.74% recall and 94.76% F1 score in recognizing the slippery，stringy and normal wrist pulses.This indicated that it had excellent performance and wide application potential in the field of multi cycle pulse recognition.

To evaluate the effect of the clearing heat，resolving phlegm and dredging Fu-organs method on secondary pulmonary infection after early gastric tube nasogastric feeding in patients with hypertensive intracerebral hemorrhage.

A hundred patients with secondary pulmonary infection after early gastric tube nasogastric feeding in hypertensive intracerebral hemorrhage were selected and random ly divide into two groups，with 50 cases in each group.The control group received basic treatment.On the basis of the control group，the observation group was treated with the method of clearing heat，resolving phlegm and dredging Fu-organs.One course of the treatment was two weeks.The therapeutic effects of two groups were compared.The inflammatory markers［interleukin-6（IL-6），tumor necrosis factor-α（TNF-α），hypersensitivity C-reactive protein（hs-CRP）］and arterial blood gas indicators［partial pressure of oxygen（PaO₂），partial pressure of carbon dioxide（PaCO₂）］before and after treatment were used to evaluate clinical pulmonary infection score（CPIS）.

The clinical efficacy of the observation group was better than that of the control group，and the difference was statistically significant（P<0.05）.The serum levels of IL-6，TNF-αand hs-CRP in the observation group after 2 weeks of treatment were lower than those of the control group and the difference was statistically significant（P<0.05）.After 2 weeks of treatment，the value of PaO₂ in the observation group was higher than that in the control group，and the value of PaCO₂ was lower than that in the control group，with a statistically significant difference（P<0.05）.The CPIS score of the observation group was lower than that of the control group after 1 and 2 weeks of treatment，and the difference was statistically significant（P<0.05）.

The clearing heat，resolving phlegm and dredging Fu-organs method has a definite control effect on secondary pulmonary infection after early gastric tube nasogastric feeding in hypertensive intracerebral hemorrhage，which helps to inhibit inflammation，improve arterial blood oxygen and reduce the degree of infection.

To analyze the effect of Ningxin Anshen Decoction（宁心安神汤）on attention deficit hyperactivity disorder（ADHD）under the guidance of brain-gut axis theory on intestinal flora.

A total of 102 children with attention deficit hyperactivity disorder were included in the study.They were divided into two groups by random number table method.Fifty-one cases in the control group were treated with Methylphenidate Hydrochloride Tablets，and 51 cases in the observation group were treated with Ningxin Anshen Decoction on this basis.The difference of efficacy，symptom score，Attention Deficit Hyperactivity Disorder Scale（SNAP-Ⅳ），number vanishing test（NCT）error rate，child Behavior Scale（CBCL）and intestinal flora index were compared between the two groups.

The total effective rate of the observation group was 96.08%（49/51），which was much higher than 78.43% （40/51）of the control group（P<0.05）.After treatment，the scores of hyperactivity，restlessness，distraction，polyglot and irritability in the observation group were lower than those in the control group，and the SNAP-Ⅳscore and NCT error rate in the observation group were lower than those in the control group，and the CBCL score was higher than that in the control group（P<0.05）.After treatment，the levels of Bifidobacterium，Lactobacillus and Faecalis in observation group were higher than those in control group，and Enterococcus was lower than those in control group（P<0.05）.

-Ningxin Anshen Decoction under the guidance of brain-gut axis theory have a clear effect in the treatment of attention deficit hyperactivity disorder，which can adjust the balance of intestinal flora，increase the contents of Bifidobacterium and Lactobacillus，effectively improve the symptoms of children，the attention ability and cognitive function，and has high application value.

To explore the clinical efficacy of Tongbian Decoction（通便汤）in treating functional constipation patients with Qi and Yin deficiency type by regulating the brain-gut-microbiota axis signaling pathway and analyze its mechanism of action.

Seventy-four patients with functional constipation who visited the Department of Colorectal and Traditional Chinese Medicine of Dangshan County People´s Hospital from January 2023 to December 2023 were selected.They were randomly divided into a Western medicine group and a Chinese medicine group using a random number table method，with 37 patients in each group.The Western medicine group was treated with Pulucabili Tablets，while the Chinese medicine group was treated with Tongbian Decoction.The clinical efficacy，symptoms，psychological status and adverse reactions of two groups as well as the changes in gut microbiota and brain gut peptide levels before and after treatment were compared.

The effective rate in the Western medicine group was 64.86%（24/37），while that in the Chinese medicine group was 83.79%（31/37）. Compared with the Western medicine group，the effective rate of the Chinese medicine group was significantly higher（P<0.05）.According to the severity，the symptoms scored 0-3 points in order.After 4 weeks of treatment，the symptom scores of fecal morphology/type，defecation interval and difficulty of defecation in the Chinese medicine group were significantly lower than those in the Western medicine group（P<0.01）.After the intervention，the scores of Hamilton Anxiety Scale（HAMA）and Hamilton Depression Scale（HAMD）in Chinese medicine group were significantly lower than those in western medicine group（P<0.01）. Compared with those in western medicine group，the levels of serum 5-hydroxytryptamine（5-HT），vasoactive intestinal peptid（VIP）and neuropeptide Y（NPY）in Chinese medicine group were significantly lower with statistical significance（P<0.01）.There was no significant difference in the levels of Bifidobacterium，Lactobacillus or Faecalibacterium prausnitzii between the two groups before intervention（P>0.05）.After the intervention，the levels of Bifidobacterium，Lactobacillus and Faecalibacterium prausnitzii in the Chinese medicine group were significantly higher than those in the Western medicine group.The main adverse reactions of all patients during the intervention process were nausea，vomiting，loss of appetite，abdominal pain，diarrhea，dizziness and headache.There was no significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Tongbian Decoction has a good clinical effect in treating functional constipation by regulating the brain-gut-microbiota axis signaling pathway.It can improve the psychological status of patients，regulate the gut microbiota and serum levels of brain gut peptides such as 5-HT，VIP and NPY，and there is no increase in adverse reactions.

To observe the clinical efficacy of Ding´s Benefiting Qi and Nourishing Yin Decoction（益气养阴汤）in the treatment of prolonged menstruation due to deficiency of both Qi and Yin.

This study selected 108 patients with deficiency of both Qi and Yin type of menstrual extension who were treated in the gynecology department of The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from September 2021 to September 2022 as the subjects.They were randomly divided into two groups using a random number table.Both groups received basic treatment.The control group received the treatment with Callicarpa nudiflora Dispersible Tablets，and the observation group received treatment with Ding´s Benefiting Qi and Nourishing Yin Decoction.Both groups were treated continuously for three courses of treatment.The differences in therapeutic efficacy，menstrual time，recurrence rate and sex hormones，traditional Chinese medicine symptom score，duration of basal body temperature（BBT）and high temperature phase，fibrinogen（FIB）and platelet count（PLT）were compared between two groups.

The total effective rate of the observation group was higher than that of the control group，and the overall menstrual time and average menstrual time were shorter than those of the control group，with statistical differences（P<0.05）.There was no statistically significant difference in the TCM symptom scores between the two groups before treatment（P>0.05）.After treatment，the scores of fatigue，dry throat and mouth，lack of breath and lazy speech，restlessness and heat，self sweating，constipation，dizziness，blurred vision and short red urine in both groups decreased compared to those before treatment（P<0.05）.The TCM symptom score of the observation group after treatment was lower than that of the control group（P<0.05）.There was no statistically significant difference in sex hormones between the two groups before treatment（P>0.05）.After treatment，the levels of estradiol（E2），luteinizing hormone（LH）and progesterone（P）decreased in both groups compared to those before treatment.However，there was no statistically significant difference in follicle stimulating hormone（FSH）levels compared to those before treatment（P>0.05）.After treatment，the levels of E2，P and LH in the observation group were lower than those in the control group，and there was no statistically significant difference in FSH compared to that of the control group（P>0.05）.There was no statistically significant difference in the duration of BBT hyperthermia phase，FIB or PLT between the two groups before treatment（P>0.05）.After treatment，the duration of high temperature phase of BBT decreased in both groups compared to that before treatment，while PLT increased compared to that before treatment.There was no statistically significant difference in FIB compared to that before treatment（P>0.05）.After treatment，the duration of BBT hyperthermia phase in the observation group was lower than that in the control group，while PLTwas higher than that in the control group.There was no statistically significant difference in FIB compared to that of the control group（P>0.05）.The recurrence rate of the observation group was 14.89%（7/54），which was lower than that of the control group（37.04%，20/54），with a statistical difference（P<0.05）.

In the treatment of prolonged menstruation due to deficiency of both Qi and Yin，Ding´s Benefiting Qi and Nourishing Yin Decoction can reduce symptoms，regulate sex hormone levels，improve the therapeutic efficacy，and reduce the recurrence rate.

To explore the effect of Bailing Capsule（百令胶囊）combined with aminophylline in the treatment of chronic obstructive pulmonary（COPD）in stable phase and the effect on the expression of Kelch like epichlorohydrin related protein-1（Keap1）-nuclear transcription factor erythroid 2p45（Nrf2）-antioxidant response element（ARE）signaling pathway，so as to provide effective reference for clinical medication.

A prospective randomized controlled study of 112 patients with stable COPD in the hospital from February 2020 to March 2023 were selected and divided into combination group（56 cases）and control group（56 cases）by random number table.Both groups were given conventional treatment.On this basis，the control group was given aminophylline，and the combination group was given Bailing Capsule combined with aminophylline，both of which were treated for 3 months.The efficacy，the 6-min walking distance，changes in dyspnea index（TDI）and lung function［expiratory volume in the first second（FEV₁），forced vital capacity（FVC），FEV₁/FVC］，serum inflammatory factor indicators［tumor necrosis factor-α（TNF-α），interleukin-6（IL-6），high-sensitivity C-reactive protein（hs-CRP）］，immune function indexes（CD3⁺，CD4⁺，CD4⁺/CD8⁺）and the expression of Keap1-Nrf2-ARE signal pathway before and after treatment of the two groups were compared.

（1）Efficacy and symptom improvement：The total effective rate of the combination group was 91.07%（51/56），which was higher than that of the control group（75.00%，42/56）.After treatment，the TDI was higher than that of the control group（P<0.05）.（2）Sports endurance and lung function：After treatment，the 6-min walking distance，the levels of FEV₁，FVC and FEV₁/FVC in both groups increased compared to those before treatment，and the indexes of the combination group were higher than those of the control group（P<0.05）.（3）Inflammatory response：Compared with those before treatment，after treatment，the serum levels of inflammatory factors such as TNF-α，IL-6 and hs-CRP were significantly reduced，with a higher decrease in the combination group（P<0.05）.（4）Immune function：After treatment，the levels of CD3⁺，CD4⁺and CD4⁺/CD8⁺in the combination group were higher than those in the control group（P<0.05）.（5）Keap1-Nrf2-ARE signaling pathway：After treatment，the expression levels of Keap1，Nrf2 and ARE proteins increased in both groups，with the combination group showing a more significant increase（P<0.05）.

The combination of Bailing Capsule and aminophylline has a significant clinical effect in the treatment of stable COPD，which can effectively promote the improvement of patients’symptoms，improve lung function and exercise endurance，while reducing the body´s inflammatory response，improving immune function and up-regulating the expression of Keap1-Nrf2-ARE signaling pathway.

To explore the clinical effect of Qisheng Gushen Decoction（芪参固肾汤）combined with irbesartan in the treatment of chronic kidney disease at stage 2～3.

A total of 110 patients with chronic kidney disease at stage 2～3（admitted from August 2021 to October 2023）were divided into decoction group and western medicine group by random number table method，with 55 cases in each group.The western medicine group received irbesartan and other western medicines，and the decoction group was treated with Qisheng Gushen Decoction on the basis of the western medicine group.In the course of treatment，1 case was shed in the western medicine group and 2 cases were shed in the decoction group.The data of each group was observed as follows：curative effect，integral changes of TCM symptoms（main disease and secondary disease）before and after treatment，changes of renal function indicators，changes of inflammatory factors indicators，changes of intestinal flora，changes of T lymphocyte subsets and other immune function indicators，adverse reactions.

The total effective rate of the decoction group was significantly higher than that of the western medicine group（P<0.05）.After treatment，the scores of TCM syndrome（main disease and secondary disease），24-hour urinary protein quantification（24 h-UTP），serum creatinine（SCr），urea nitrogen（BUN），albumin（ALB），estimated glomerular filtration rate（eGFR），interleukin-6（IL-6），interleukin-17（IL-17）and hypersensitive C-reactive protein（hs-CRP），intestinal flora indicators such as Lactobacillus acidophilus，bifidobacterium and Escherichia coli，and immune indicators such as T lymphocyte subsets（CD3⁺，CD4⁺，CD8⁺，CD4⁺/CD8⁺）were improved.In the decoction group，these indexes were all better than those in western medicine group（P<0.05）.The total adverse reaction rate of the decoction group was lower than that of the western medicine group（P<0.05）.

Qisheng Gushen Decoction combined with irbesartan in the treatment of chronic kidney disease at stage 2～3 can regulate the immune function，improve the renal function，immune function and inflammatory response of patients，enhance the therapeutic effect，and reduce adverse reactions，and the treatment is safe and reliable.

Based on the “brain-derived neurotrophic factor（BDNF）/phospho-tropomyosin receptor kinase B（TrkB）/p38/c-Jun N-terminal kinase（JNK）”signaling pathway，this study aimed to investigate the mechanism of electroacupuncture stimulation of pain points in the treatment of myofascial pain syndrome（MPS）.

SD rats were divided into normal group，model group，electroacupuncture group and western medicine group.MPSmodel rats were established by a combination of percussion and centrifugal exercise，followed by electroacupuncture stimulation of pain points and oral administration of celecoxib for intervention.After the experiment，the morphology of muscle tissues in rats of each group was observed by hematoxylin-eosin（HE）and Masson staining.Prussian blue staining was used to observe the degranulation of mast cells in the muscle tissues of each group.TUNEL fluorescence staining was used to observe the cell apoptosis in the muscle tissues of each group.Reverse Transcription Polymerase Chain Reaction（RT-PCR）was used to detect the mRNA expression levels of Bcl-2 associated X protein（Bax），B-cell lymphoma-2（Bcl-2），cysteine aspartate-specific protease 3（Caspase-3），BDNF，TrkB，p38 and JNK in the muscle tissues of each group.Wes was used to detect the protein expression levels of Bax，Bcl-2，Caspase-3，BDNF，p-TrkB，p-p38 and p-JNK in the muscle tissues of each group.

HE and Masson staining indicated that both electroacupuncture and celecoxib intervention can improve muscle pathological damage caused by MPS，and can inhibit mast cells degranulation and apoptosis of muscle cell.Electroacupuncture and celecoxib intervention can increase Bcl-2 mRNA expression and decrease the mRNA expressions of Bax，Caspase-3，BDNF，TrkB，p38 and JNK，increase Bcl-2 protein expression and decrease protein expressions of Bax，Caspase-3，BDNF，p-TrkB，p-p38 and p-JNK.Electroacupuncture was more effective than celecoxib.

Electroacupuncture can treat MPS by inhibiting the BDNF/Trkb/p38/JNK signaling pathway，thereby reducing cell apoptosis in MPSaffected muscle tissue.In traditional Chinese medicine theory，electroacupuncture can be used to restore the imbalance of Qi，blood，Yin and Yang in the body，but the corresponding mechanism still needs further exploration.

This study aimed to explore the efficacy of Centella Asiatica Cream in treating hypertrophic scars.

A total of 107 patients with keloid lesions were included.Following a double-blind allocation principle，the patients were divided into an experimental group（49 cases）and a control group（58 cases）.The Vancouver Scar Scale（VSS）and the Patient and Observer Scar Assessment Scale（POSAS）were employed to evaluate changes in color，hardness，height，vascularization，texture and pain before and after treatment in both groups.

At the 12th and 24th week of treatment with Centella Asiatica Cream，the experimental group demonstrated statistically significant differences in keloid scar diameter，pigmentation，thickness，vascular distribution and softness according to the VSS compared with those of the control group（P<0.05）.The POSAS observer scale indicated significant statistical differences between the two groups at the 12th and 24th week（P<0.05），while the POSAS patient scale showed significant differences at the 4th，12th and 24th week（all P<0.05）.

Centella Asiatica Cream formulation showed significant efficacy in the repair of hypertrophic scars.

In order to better meet people´s health needs and provide a useful reference for the high-quality development of Wuweizi（Schisandra chinensis）industry in China.

This paper systematically summarized the present value chain situation of Wuweizi（Schisandra chinensis）industry in China.

It summarized the basic situation of Wuweizi（Schisandra chinensis）industry in China in four aspects，including planting industry，Chinese patent medicine industry，circulation and knowledge industries.

Countermeasures for the development of Wuweizi（Schisandra chinensis）industry in China have been proposed，which should strengthen the management of Wuweizi（Schisandra chinensis）and realize large-scale planting and production，strengthen the brand construction of Wuweizi（Schisandra chinensis）and expand the international market，and increase the scientific and technological research to promote the sustainable development of the Wuweizi（Schisandra chinensis）industry.

To observe the clinical effect of prone position cervical spine fine-tuning combined with tendon-regulation manipulation on three Yang meridians of hand on cervical spondylotic radiculopathy（CSR）and its influence on infrared thermography temperature.

A total of 60 patients with CSR were randomly divided into two groups.The control group received electroacupuncture treatment，while the observation group received electroacupuncture treatment combined with prone position cervical spine fine-tuning and tendon-regulation manipulation on three Yang meridians of hand.The visual analog scale（VAS），Northwick park neck-pain questionnaire（NPQ），neck disability index（NDI），clinical assessment scale for cervical spondylosis（CASCS），cervical range of motion（ROM）scores and infrared thermal imaging temperature difference of the two groups were compared before and after 2 weeks of treatment.

After treatment，the scores of VAS，NPQ，NDI，CASCS and ROM and infrared thermal imaging temperature difference of both groups improved compared to those before treatment（P<0.05），and the observation group has better scores in various aspects（P<0.05）.

Prone position cervical spine fine-tuning combined with tendon-regulation manipulation on three Yang meridians of hand for the treatment of CSR has significant therapeutic effects.The changes in infrared temperature can provide an objective and direct basis for the evaluation.

To investigate the effect of dihydromyricetin（DMY）on proliferation and metastasis of esophageal squamous cell carcinoma（ESCC）and whether it is mediated by the LncRNA CASC9/miR-195-5p/E2F7 axis.

Gradient concentrations（0，25，50，100μmol/L）of DMY were used to treat ESCC cells in order to investigate the effects of DMY on ESCC cells proliferation，migration and invasion.The possible interaction between the LncRNA CASC9/miR-195-5p/E2F7 axis in ESCC was predicted using bioinformatics.Real-time quantitative polymerase chain reaction（RT-qPCR）assay was used to detect the expressions of LncRNA CASC9，miR-195-5p and E2F7 in cells，and the regulation relationship was confirmed using RT-qPCR，Western blot and double luciferase reporter gene assays.In order to test the cell viability，proliferation，migration and invasion ability，cell counting kit-8（CCK-8），colony formation，wound healing and Transwell invasion assays were used respectively.Western blot assay was used to test the expressions of epithelial mesenchymal transition（EMT）markers E-cadherin，NCadherin and Vimentin.

The proliferation，migration and invasion abilities of ESCC cells were significantly inhibited by DMY.LncRNA CASC9 was hyperexpressed in ESCC by bioinformatics analysis and cell level verification.The proliferation，migration and invasion abilities of ESCC cells were inhibited by DMY treatment leaded to by down-regulating the expression level of LncRNA CASC9 in ESCC cells.Furthermore，the miR-195-5p expression was down-regulated in ESCC while E2F7 expression was up-regulated.RT-qPCR，Western blot and double luciferase reporter gene assays all confirmed that LncRNA CASC9 regulated E2F7 expression by adsorbing miR-195-5p.E2F7 knocking down-reversed the promotion of miR-195-5p knocking down on the proliferation，metastasis and EMT process of ESCC，whereas E2F7 overexpression reversed the inhibition of miR-195-5p overexpression.

DMY inhibits ESCC proliferation and metastasis via the LncRNA CASC9/miR-195-5p/E2F7 axis.

To analyze the effects of acupuncture and moxibustion combined with a novel adjustable three-point knee joint traction device on pain，function and inflammation indexes in patients with degenerative knee osteoarthritis.

A total of 180 patients with degenerative knee osteoarthritis were included as the study objects.They were divided into 3 groups by random number table method：acupuncture and moxibustion group（60 cases）was treated with acupuncture and moxibustion，traction group（60 cases）was treated with adjustable three-point knee traction，and combined group（60 cases）was treated with acupuncture and moxibustion combined with adjustable three-point knee traction.The treatment effect，symptom score，pain，joint function，inflammatory factors，knee distance and tibiofemoral angle were compared between the two groups.

The total effective rate of the combined group was96.67%（58/60），which was much higher than that of acupuncture and moxibustion group（80.00%，48/60）and traction group（81.67%，49/60），and the difference was significant（P<0.05）.After treatment，the combined therapy group showed significantly lower TCM symptom scores-including joint pain，joint swelling，restricted flexion-extension，and soreness and weakness in the lumbar region and knees-as well as lower VASscores，compared with the acupuncture group and the traction group（P<0.05）.After treatment，the Lequesne MG score and WOMAC score of the combined group were lower than those of the acupuncture and moxibustion group and traction group（P<0.05）.After treatment，the levels of interleukin-6（IL-6），interleukin-1β（IL-1β）and hypersensitive C-reactive protein（hs-CRP）in the combined group were lower than those in the acupuncture group and moxibustion and traction group（P<0.05）.After treatment，the tibiofemoral angle of the combined group was greater than that of the acupuncture and moxibustion group and traction group，and the knee distance was smaller than that of acupuncture group and traction group，and the difference was significant（P<0.05）.

Acupuncture and moxibustion combined with adjustable three-point knee traction for patients with degenerative knee osteoarthritis has a clear effect，which can effectively improve the symptoms of patients，reduce the degree of pain，reduce the levels of inflammatory factors，reduce the expressions of hs-CRP，IL-1βand IL-6 by correcting the internal and external inversion of the knee joint，improve the mobility of the knee joint and promote the functional recovery of the knee joint.

Based on the theory of “the heart is connected with the small intestine” in traditional Chinese medicine，this study explored the mechanism of gut microbiota driving the TMA/FMO3/TMAO pathway to affect platelet aggregation in acute coronary syndrome（ACS）with syndrome of phlegm and blood stasis rats.

Forty-five SPF healthy SD rats were randomly divided into control group（CON），ACSgroup and ACSwith syndrome of phlegm and blood stasis group（TYACS），with 15 rats in each group.Eight-channel multi-lead electrocardiogram was used to record the changes of leadⅡelectrocardiogram of the standard limb of rats.The serum lipid levels of rats were detected by automatic biochemical analyzer.HE staining was used to observe the structural changes of myocardial tissue.The maximum aggregation rate of platelet arachidonic acid（AA）and adenosine diphosphate（ADP）were detected by photoelectric turbidity method.LC/MS technology was used for detecting the levels of trimethylamine（TMA）and trimethylamine N-oxide（TMAO）in the plasma.Flavin containing monooxygenase 3（FMO3）gene and protein expressions in liver were detected by RT-PCR and Western blot.Spearman correlation analysis was used to investigate the correlation between TMAO and the maximum aggregation rate of platelet AA and ADP.Metagenomic technology was used to detect the characteristics of gut microbiome in rats.

The ECG changes of rats in the ACS group and the TYACS group were consistent with ACS，and the serum lipid levels of rats in the TYACSgroup were consistent with the dietary imbalance of the syndrome of phlegm and blood stasis.HE staining showed that the myocardium of the CON group was arranged neatly and the cytoplasm was abundant.In the ACS group and the TYACSgroup，the inflammatory cell infiltration in the ischemic area，the loss of myocardial nucleus，the vacuolar degeneration of myocardial cells and the disorder of myocardial organization were observed.The maximum aggregation rate of platelet AA and ADPwere significantly increased in the ACS and TYACSgroups（P<0.01）.LC/MS showed that the levels of TMA and TMAO in ACS group and TMA in TYACS group were showed an upward trend，and the level of TMAO in TYACSgroup was significantly increased（P<0.001）.FMO3 mRNA and protein in liver of the ACS and TYACS groups were significantly increased（P<0.01）.Maximum aggregation rate of platelet AA and ADP were positively correlated with TMAO level.Metagenomic analysis revealed that the characteristics of gut microbiome in the ACSand TYACS groups were significantly changed.The species markers significantly different in the CON group was Bacteroidetes，in the ACSgroup were Firmicutes and Fusobacteria，in the TYACS group were Proteobacteria，Actinobacteria and Spirochaetes.KEGG enrichment analysis showed that the bacteria were most enriched in AA metabolism，riboflavin metabolism and other pathways in ACSwith syndrome of phlegm and blood stasis rats，and the differences were the most obvious.ACS rats were especially related to AA metabolism，insulin resistance，riboflavin metabolism and other pathways.

The gut microbiome of rats with ACS and ACSwith syndrome of phlegm and blood stasis had significant characteristic changes.The platelet aggregation process of model rats may be related to a variety of different gut microbiota driving the TMA/FMO3/TMAO pathway，participating in AA metabolism，riboflavin metabolism，etc.This finding may provide a theoretical basis for exploring new methods to improve platelet aggregation function and prevent ACSby regulating flora forest，and enrich the theory of “the heart is connected with the small intestine” in traditional Chinese medicine.

To observe the effects of Kangxian Yixin Decoction（抗纤益心方，KYD）on myocardial mitochondrial ridge and its structural proteins mitochondrial inner membrane ridge structure protein 10（Mic10），mitochondrial inner membrane ridge structure protein 60（Mic60）and mitochondrial inner membrane ridge structure protein 19（Mic19）in mice with dilatedcardiomyopathy（DCM），and explore the mechanism of protecting DCM mitochondrial ridge structure and improving mitochondrial energy metabolism.

According to random number table method，40 cTnTR141W transgenic DCM mice were divided into model group，KYD low-dose group，KYD medium-dose group，KYD high-dose group and captopril group，with 8 mice in each group，and 8 C57BL/6J mice in normal group.Left ventricular end-systolic diameter（LVESD），left ventricular end-diastolic diameter（LVEDD），ejection fraction（EF）and fraction shorting（FS）were detected by ultrasonography.Myocardial pathological morphology was observed by HE staining，and mitochondrial ultrastructure was observed by electron microscopy.Enzyme-linked immunosorbent assay（ELISA）was used to detect the contents of NT-proBNP and adenosine triphosphate（ATP）in serum of mice.The mRNA and protein expressions of Mic10，Mic60 and Mic19 were detected by Real-time PCR and Western blotting，respectively.

Compared with those in the normal group，in the model group，the levels of LVESD and LVEDD were increased，the levels of FS and EF were decreased（P<0.05），myocardial pathological injury was observed，apoptotic number was increased（P<0.01），NT-proBNP content was increased，ATP content was decreased（P<0.01），mitochondrial ridge structure was damaged and disordered.The mRNA and protein expressions of mitochondrial ridge structure proteins Mic10，Mic60 and Mic19 were decreased（P<0.01）. Compared with those in the model group，in the KYD group，the levels of LVESD and LVEDD were decreased，the levels of FS and EF were increased（P<0.05），the myocardial injury was alleviated，the NT-proBNP content was decreased（P<0.01），the apoptosis was decreased，the ATP content was increased（P<0.05），the mitochondrial ridge damage and arrangement disorder were improved and the mRNA and protein expressions of mitochondrial ridge structure proteins Mic10，Mic60 and Mic19 were increased（P<0.01）.

KYD can improve DCM mice mitochondrial ridge arrangement disorder，alleviate myocardial cell apoptosis，and improve cardiac function，which may be related to increasing the expression of mitochondrial ridge structural proteins Mic10，Mic60 and Mic19 and improving mitochondrial energy metabolism.

To investigate the effect of shikonin on the cell proliferation，migration，invasion and apoptosis of nasopharyngeal carcinoma（NPC）cells by regulating miR-224 expression.

Thirty NPC patients treated in the hospital from January 2023 to March 2024 were selected and underwent surgery.The adjacent and cancerous tissues were removed during the surgery.The normal cultured human SUNE-1 cells were recorded as the blank control group，those treated with shikonin were designated as the shikonin group.Those treated with shikonin and transfected with miR-NC and miR-224 mimetics were designated as the miR-NC and miR-224 groups.The expression of miR-224 in each group was compared，and the effects of shikonin on cell proliferation，migration，invasion and apoptosis were observed by regulating miR-224 expression.

The expression of miR-224 in NPC tissue was（3.23±0.16），which was higher than that（1.09±0.07）in adjacent tissues（P<0.05）. Comparison of miR-224 expression among the groups：shikonin+miR-224 group>blank control group>shikonin+miR-NC group>shikonin group，and the pairwise comparisons among groups were statistically significant（P<0.05）. Comparison of optical density（OD）value，number of migrating cells and number of invading cells among the groups：blank control group>miR-224 group>miR-NC group>shikonin group. Comparison of apoptosis rate among the groups：blank control group<miR-224 group<miR-NC group<shikonin group.There were statistically significant pairwise comparisons among groups（P<0.05）. Comparison of CyclinD1 protein，matrix metalloproteinase2（MMP-2）protein，MMP-9 protein and B-cell lymphoma-2（Bcl-2）protein among the groups：blank control group>miR-224 group>miR-NC group>shikonin group. Comparison of cyclin-dependent kinase inhibitor 1A（p21）protein and Bcl-2 associated X protein（Bax）protein among the groups：blank control group<miR-224 group<miR-NC group<shikonin group.There were statistically significant pairwise comparisons among groups（P<0.05）.

Shikonin can reduce the expression of miR-224 in NPC patients，inhibit the cell proliferation，migration and invasion，and induce apoptosis.

To systematically evaluate the short-term safety and efficacy of Shexiang Baoxin Pill（麝香保心丸）combined with dual antiplate therapy in the treatment of elderly female patients with acute myocardial infarction.

90 female patients with acute myocardial infarction were collected.They were divided into observation group（group A，45 cases）and control group（group B，45 cases）by random number table method.The observation group was treated with Shoxiang Baoxin Pill combined with dual antiplate therapy.The control group was treated with conventional dual antiplate therapy.Both groups received routine hospital treatment for 2 weeks and were discharged with symptoms improved.After discharge，they took medicine regularly according to the doctor´s advice.The data before treatment and one month after discharge was recorded respectively.The efficacy indicators were angina pectoris efficacy score，Herth hope scale and QLQ-C30（quality of life survey rating table）.The safety indicators included platelet count，P-D score（PRECISE-DAPT score，or prediction of bleeding complications in patients undergoing coronary stent implantation and subsequent dual antiplatelet therapy），liver function（alanine aminotransferase，aspartate aminotransferase），kidney function（creation），bleeding，recurrence of myocardial infarction，death and other adverse reactions.The clinical efficacy and safety of Shexiang Baoxin Pill combined with dual antiplate therapy in the treatment of elderly female patients with acute myocardial infarction were analyzed.

1.There were no significant differences in the values of alanine aminotransferase，aspartate aminotransferase，platelet or creatinine between the two groups before treatment（P>0.05）.After treatment，the observation group showed a decrease in alanine aminotransferase，while the control group showed a slight increase in alanine aminotransferase，with no statistically significant difference between the two groups（P>0.05）.After treatment，the control group showed a decrease in aspartate aminotransferase，while the observation group showed no significant change in aspartate aminotransferase，and there was no statistical difference between the two groups（P>0.05）.After treatment，the platelet count in both groups decreased，and there was no statistically significant difference between the two groups（P>0.05）.After treatment，the creatinine levels in both groups increased and there was no statistically significant difference between the two groups（P>0.05）.2.There was no statistical significance in QLQ-C30，angina pectoris score or Herth score between the two groups before treatment（P>0.05）.After treatment，the QLQ-C30 scores and angina pectoris scores of the two groups decreased significantly，the Herth scores of the control group decreased，and that of the observation group increased.The angina pectoris score of the observation group was significantly lower than that of the control group after treatment with statistical significance（P<0.05），but the QLQ-C30 score and Herth score after treatment had no statistical significance（P>0.05）.3.There was no statistical significance in PD scores of the two groups before treatment（P>0.05），and PD scores of the two groups after treatment were significantly decreased compared with those before treatment，and the score of the observation group was significantly better than that of the control group（P<0.05）.4.There was no significant difference in adverse events between the two groups（P>0.05）.

Shexiang Baoxin Pill combined with dual antiplate therapy can significantly reduce angina pectoris score and improve angina pectoris symptoms in the treatment of elderly female patients with myocardial infarction，and it is safe and effective，without increasing the risk of bleeding and the burden of liver and kidney.

To compare the toxic effects of glycosides of Leigongteng（Tripterygium wilfordii）（GTW）on liver，blood and reproductive system in male rats of different ages.

Fifty-six male Sprague-Dawley（SD）rats aged 3，5 and 10 weeks and 8 months（equivalent to pre-school age，school age，adolescence and adulthood）were selected.The each age group was divided into control group and GTW group by random number table method.The GTW group was given 12.5 mg/（kg·d）［equivalent to2 mg/（kg·d）for children］GTW suspension，and the control group was given the same amount of normal saline.Seven rats in each age group were taken from the control group and GTW group at 2，4，8 and 12 weeks，respectively，and the organ indexes of liver，testis and epididymis were calculated.Blood routine indexes were detected by automatic blood cell analyzer.Liver function indexes were detected by automatic dry biochemical analyzer.The serum levels of testosterone（T），follicle stimulating hormone（FSH），luteinizing hormone（LH）and estradiol（E2）were detected by ELISA.The histopathological changes of liver，testis and epididymis were observed by HE staining.Sperm density and viability were calculated under light microscope.ImajeJ software counted the proportion of spermatogonia，spermatocytes and spermatids in the seminiferous tubules of the testis.

From the perspective of the liver system，there was no significant increase in liver index in rats of all age groups during GTW intervention.Alanine aminotransferase（ALT）and aspartate aminotransferase（AST）increased at the 4th and 8th week（P<0.05），and recovered at the 12th week.The pathological morphology of the liver showed that the degree of liver damage increases with the time of gastric lavage，and there was no significant difference between different age groups.From the perspective of the blood system，a decrease in PLT（P<0.05）appeared earliest and recovered by the 12th week.The rats aged 5 and 10 weeks showed a decrease in white blood cell（WBC）at 4th week（P<0.05）and recovered at 12th week.The decrease in hemoglobin（HB）（P<0.05）appeared the latest and all except for 3-week-old rats recovered at 12th week.From the perspective of the reproductive system，5-week-old and 8-month-old rats showed a decrease in T at the 4th week（P<0.05），followed by a decrease in T in all rats（P<0.05）.Except for 3-week-old rats，all other rats showed a decrease in sperm density in the second week（P<0.05），while 8-month-old rats showed the earliest decrease in sperm density and viability in the 4th week（P<0.05）.In the second week，8-month-old rats showed a decrease in the proportion of sperm cells at the earliest stage（P<0.05）.Pathology of the testes and epididymis both showed severe damage in 8-month-old rats.

①There was no significant difference in liver damage caused by GTW in rats of different age groups，but it worsened with medication time.②The toxicity of GTW to the blood system is manifested by inconsistent decreases in blood routine indicators，and young rats are more intolerant to blood damage caused by GTW than adult rats.③Compared to young rats，adult rats are more sensitive to the reproductive toxicity of GTW.

To investigate the protective effect and mechanism of Schisandrae Mixture（五味子合剂，SM）on the kidneys of diabetic nephropathy（DN）rats.

Sixty male SPF SD rats were randomly divided into control group，model group，dapagliflozin（DA）group，SM high-dose（SM-H）group，SM medium-dose（SM-M）group and SM low-dose（SML）group.The model was induced by a high-sugar and high-fat diet combined with streptozotocin（STZ）.Blood glucose（Glu）and 24-hour urinary protein（24 h UTP）were measured every 4 weeks.After 12 weeks of administration，the kidney was weighed，the serum and kidney tissue were collected，and the kidney index（KI）was calculated.The levels of serum creatinine（Scr）and blood urea nitrogen（BUN）were detected.Hematoxylin-eosin（HE），silver hexamine（PASM）and Masson staining were used to observe the pathological morphology of renal tissue.The levels of malondialdehyde（MDA），superoxide dismutase（SOD）and glutathione（GSH）in renal tissue were detected by corresponding kits.The mRNA and protein expression of hypoxia-inducible factor-1α（HIF-1α）and heme oxygenase-1（HO-1）in renal tissue were detected by real-time fluorescence quantitative PCR（qRT-PCR）and Western Blotting（WB）.The levels of glutathione peroxidase 4（GPX4）and acyl-CoA synthetase long-chain family member 4（ACSL4）in renal tissue were detected by immunohistochemistry.

Compared with those of the control group，the pathological damage of the model group was significantly aggravated；the levels of Glu，24 h UTP，Scr，BUN and KI were significantly increased，MDA level was significantly increased，the levels of SOD and GSH were significantly decreased，HIF-1αand HO-1 protein and mRNA expression levels were significantly increased，GPX4 expression level was significantly decreased，ACSL4 expression level was significantly increased（P<0.05）. Compared with those of the model group，the pathological damage of DA group and SM group was significantly reduced；the levels of Glu，24 h UTP，Scr，BUN and KI were decreased，the level of MDA in renal tissue was decreased，and the levels of SOD and GSH were increased.Among them，the changes of the above indexes in SM-H group were the most obvious，and there was statistical significance except for BUN，MDA and SOD in SM-L group（P<0.05）.The protein and mRNA levels of HIF-1αand HO-1 in the SM-H group were significantly increased，the expression level of GPX4 was significantly increased，and the expression level of ACSL4 was significantly decreased（P<0.05）.

SM has a certain renal protective effect on DN，which may be related to regulating HIF-1α/HO-1 pathway to reduce oxidative stress and regulating GPX4/ACSL4 pathway to inhibit ferroptosis.

To investigate the protective effect and intervention mechanism of arctigenin on lipopolysaccharide（LPS）combined with adenosine triphosphate（ATP）-stimulated pyroptosis in RAW264.7 cells.

CCK8 assay was used to detect the effects of different concentrations of arctigenin on the viability of RAW264.7 cells；CCK8 assay was used to detect the effects of intervention of arctigenin（20，40，80μmol/L）on the viability of RAW264.7 cells induced by LPS+ATP；LDH release was used to detect the cellular membrane damage；ELISA was used to detect tumour necrosis factor-α（TNF-α），interleukin-6（IL-6）and IL-1βin cell supernatant；Western blotting was used to detect intracellular NLRP3，Caspase-1 p20，Caspase-1 p45，GSDMD-N and GSDMD-FL protein expression；the number of positive cells stained with propidium iodide（PI）was observed by forward and inverted fluorescence microscopy.

Arctigenin concentration less than 160μmol/L had little effect on cell viability；arctigenin（20，40，80μmol/L）intervention reversed the LPS+ATP-induced decrease in cell viability；arctigenin reduced LPS+ATP-induced release of LDH from pyroptosis cells as well as the number of PI-positive cells；arctigenin reduced LPS+ATP-induced LDH release from pyroptosis cells as well as the number of PI-positive cells；arctigenin inhibited the secretion of pro-inflammatory cytokines TNF-α，IL-6 and IL-1βby pyroptosis cells；arctigenin inhibited the expression of NLRP3，Caspase-1 p20，Caspase-1 p45，GSDMD-N and GSDMD-FL proteins in pyroptosis cells that inhibited the activation of NLRP3 inflammasome.

Arctigenin inhibits NLRP3 inflammasome activation to improve pyroptosis and alleviate inflammatory responses.

To investigate the differences in metabolic composition between the genuine and non-genuine production areas of Daguomujiangzi（Fructus Cinnamomi）.

The volatile components of 66 batches of Daguomujiangzi（Fructus Cinnamomi） were qualitatively and quantitatively analyzed by supercritical fluid extraction（SFE -CO₂） and gas chromatography-mass spectrometry（GC-MS），combined with cluster analysis（HCA），principal component analysis（PCA）and orthogonal partial least squares-discriminant analysis（OPLS-DA）.According to the characteristic coefficient of the overall variable VIP≥2，the differential metabolites between the genuine and non-genuine estate areas were screened.

The results of supercritical fluid extraction（SFE-CO₂）of the volatile oil of Daguomujiangzi（Fructus Cinnamomi）showed that its content ranged from 18% to 33%，and the content of volatile oil in the genuine production area was significantly higher than that in the non-genuine production area.The results of volatile oil metabolomics showed that there were obvious differences between the genuine and non-genuine production areas of Daguomujiangzi（Fructus Cinnamomi），among which 1，4，7，-Cyclo undecatriene，1，5，9，9-tetramethyl-Z，Z，Z-，beta-copaene，bicyclic dabanerene and terpenoids could be used as the metabolites for identifying the differences between the genuine and non-genuine production areas of Daguomujiangzi（Fructus Cinnamomi）and the metabolism of Daguomujiangzi（Fructus Cinnamomi）can be classified into two categories，namely，the South Panjiang River，North Panjiang River and Yujiang River，along the sub-watersheds of Daguomujiangzi（Fructus Cinnamomi）.The composition pattern of the South Panjiang，North Panjiang and Yujiang rivers can be classified into three chemotypes.

There were significant differences in volatile component metabolites between the genuine and non-genuine production areas，which could provide certain reference value for the subsequent identification and quality evaluation of the genuine production areas of Daguomujiangzi（Fructus Cinnamomi）.

To investigate the effects of Gualou Xiebai Banxia Decoction（瓜蒌薤白半夏汤，GXBD）on blood lipids，oxidized low-density lipoprotein（ox-LDL）and fecal bile acids in the model of early atherosclerosis（AS）in APOE gene knockout（Apoe^-/-）mice and to explore the possible mechanisms of GXBD in the prevention and treatment of early AS.

TCMSP database was used to search the chemical constituents of GXBD，PharmMapper to predict the targets.GeneCards database was searched with “Atherosclerosis（AS）”as the search term to select the AS-related targets with a score of≥1，and DAVID database was used to analyze the intersecting targets for Kyoto Encyclopedia of Genes and Genomes（KEGG）pathway enrichment.For the animal experiments of prevention-treatment mechanism，15 male Apoe^-/- mice were randomly divided into 3 groups：control group（Group A，normal diet），high-fat diet model group（Group AF，high-fat diet），GXBD intervention group（Group AF-GL，high-fat diet，GXBD 10 g·kg^-1·d^-1），and the high-fat diets were fed for 8 weeks in order to prepare a mouse model for early stage of AS，wherein pharmacological interventions were initiated from the 5^th week onwards to the end of the experiments.The serum levels of low-density lipoprotein（LDL），high-density lipoprotein（HDL），total cholesterol（TC）and triglycerides（TG），and concentrations of oxidized low-density lipoprotein（ox-LDL）in serum，liver and ileum were measured 4 hours after the last administration.The aorta was isolated for oil-red O staining.The mRNA levels of constitutive androstane receptors（CAR）and cholesterol 7α-hydroxylase（CYP7A1）of liver were detected and mRNA levels of fatty acid-binding protein（FABP6）in colon were detected，and fecal bile acids were measured.

Thirty-four active compounds of GXBD were obtained，and there were 164 intersecting targets with AS，which were mainly involved in lipid oxidation and signal transduction. Compared with those of group A，the AF group showed increased aortic lipid deposition，and the level of serum ox-LDL was significantly higher（P<0.01），the levels of mRNA expressions of CAR were significantly lower（P<0.05），the content of fecal primary bile acids was significantly higher（P<0.05），and the contents of secondary bile acids were significantly higher（P<0.05）. Compared with those of the AF group，aortic lipid deposition was reduced in the AF-GL group，serum ox-LDL levels were significantly decreased（P<0.01），the level of mRNA expression of CAR was significantly increased（P<0.05），and fecal primary bile acid content was significantly down-regulated（P<0.05）.

GXBD regulates lipid peroxidation by activating CAR and CYP7A1 in liver to promote bile acid metabolism，accelerating cholesterol catabolism to regulate oxidized lipids，lowering ox-LDL，and preventing and treating early AS.

To investigate the effect of ethanol extract of Guijianyu（Euonymus Alatus）on CCl₄-induced liver fibrosis model mice based on Rho A/ROCK signaling pathway.

Fifty C57BL/6 mice were randomly divided into normal group（NG），model group（MG），low-dose group of Guijianyu（Euonymus Alatus）（EA-L），medium-dose group of Guijianyu（Euonymus Alatus）（EA-M），high-dose group of Guijianyu（Euonymus Alatus）（EA-H），10 mice in each group.The mice in all groups except NGwere modeled by intraperitoneal injection of olive oil suspension containing25%CCl₄.At the same time of modeling，NG and MG were treated with double-distilled water by gavage，and the remaining three groups were treated with Guijianyu（Euonymus Alatus）by gavage with the corresponding doses（2 g/kg，4 g/kg and 8 g/kg，equivalent to the amount of the raw drug contained），respectively.6 weeks later，the serum and liver tissues were collected，and the liver function indexes［glutamic oxalacetic transaminase（AST），glutamate pyruvate transaminase（ALT）］were detected.ELISA method detected the levels of tumor necrosis factor-α（TNF-α），interleukin-6（L-6），hydroxyproline（HYP）and transforming growth factor-β1（TGFβ1）.HE and Masson staining were used to analyze the damage and collagen deposition in the liver of mice.The expressions of α-SMA，Collagen I and the relative protein expressions of Rho A/ROCK signaling pathway related proteins in the liver tissues were detected by Western blot.

Compared with those of MG，the serum levels of ALT，AST，TNF-αand IL-6 of EA-M and EA-H showed a decreasing trend. Compared with those of MG，the contents of Hyp and TGF-β1 in liver tissues of the Guijianyu（Euonymus Alatus）groups decreased to different degrees（P<0.05，P<0.01）.The hepatic morphology and structure，cellular inflammatory infiltration，fibrous changes and collagen deposition in the Guijianyu（Euonymus Alatus）groups were dose-dependently better than that of MG.The protein expression levels ofα-SMA，CollagenⅠand Rho A/ROCK signaling pathway related proteins in the EA-L，EA-M and EA-H were decreased compared with those of MG（P<0.05，P<0.01）. Compared with those of MG，the mRNA expressions ofα-SMA，CollagenⅠand Rho A/ROCK signaling pathway related proteins were decreased in EA-L，EA-M and EA-H（P<0.05，P<0.01）.

Ethanol extract of Guijianyu（Euonymus Alatus）has obvious enzyme-lowering，hepatoprotective and antihepatic fibrosis effects，and inhibition of the Rho A/ROCK signaling pathway is one of the mechanisms of its anti-hepatic fibrosis action.

This study aimed to compare the differences in chemical composition between Huangbai（Phellodendri Chinensis Cortex）processed with Xianqiushi（Sal Praeparatum）and Danqiushi（Depositum Urinae Praeparatum）and the difference of chemical composition in the auxiliary materials were compared.

High performance liquid chromatography（HPLC）was used to determine the contents of chlorogenic acid，phellodendrine，magnoflorine，jatrorrhizine hydrochloride，berberrubine，palmatine hydrochloride，berberine hydrochloride，obaculactone and obacunone in Huangbai（Phellodendri Chinensis Cortex），and uric acid in auxiliary material.The complexometric tritration method was used to determine the contents of sodium chloride，calcium chloride and insoluble calcium salt in auxiliary materials.

The contents of chlorogenic acid，phellodendrine，berberrubine，palmatine hydrochloride and berberine hydrochloride in Huangbai（Phellodendri Chinensis Cortex）processed with Xianqiushi（Sal Praeparatum）was found to be higher than that processed with Danqiushi（Depositum Urinae Praeparatum）.The contents of sodium chloride and calcium chloride in the ingredients of auxiliary materials，Huangbai（Phellodendri Chinensis Cortex）processed with Xianqiushi（Sal Praeparatum）was more than Huangbai（Phellodendri Chinensis Cortex）processed with Danqiushi（Depositum Urinae Praeparatum）.

This simple yet reliable method provides a stable approach for comprehensively comparing the differences in chemical composition between two types of Huangbai（Phellodendri Chinensis Cortex）processed with Xianqiushi（Sal Praeparatum）and Danqiushi（Depositum Urinae Praeparatum）.

To observe the curative effect of benefiting vital Qi and nourishing heart method（back Shu-acupoint warm acupuncture）on the degenerative degeneration of knee joint with insufficient kidney Yang.

A total of 80 patients with knee degenerative degeneration with insufficient kidney Yang（admitted from May 2021 to December 2022）were included in the study and divided into control group（40 cases）and observation group（40 cases）by random number table method.The patients in the control group received knee replacement treatment.The observation group was treated with benefiting vital Qi and nourishing heart method（back Shu-acupoint warm acupuncture）on the basis of control group.The data of each group was observed，including the rate of excellent and good curative effects，the TCM symptom scores before and after treatment，the pain score of patients 3 d，6 d and 9 d（visual analogue scale，VAS），and the levels of C-reactive protein（CRP），tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6），Lysholm knee scale scores before and after treatment，the incidence of complications and the the MOS item short from health survey（SF-36）before treatment，2 and 4 weeks of treatment health survey.

The rate of excellent and good treatment effect in the observation group（95.00%，38/40）was higher than that in the control group（80.00%，32/40）（P<0.05）.After treatment，TCM symptom scores decreased，inflammatory factors（CRP，TNF-α，IL-6）decreased significantly，and total Lysholm knee scale score increased significantly.TCM symptom scores and inflammatory factors（CRP，TNF-α，IL-6）in the observation group were lower than those in the control group.The total score of Lysholm knee scale was higher than that of control group（P<0.05）.VAS scores of the observation group were lower than those of the control group at 3 d，6 d and 9 d after surgery（P<0.05）. Compared with that of the control group（20.00%，8/40），the complication rate of the observation group（5.00%，2/40）was lower（P<0.05）.After 2 and 4 weeks of treatment，the SF-36 score of the observation group was higher than that of the control group（P<0.05）.

The benefiting vital Qi and nourishing heart method（back Shu-acupoint warm acupuncture）has a good effect on the treatment of kidney Yang deficiency type of knee degenerative diseases.The patient´s symptoms and the knee joint function are improved with less complications.It is a safe and reliable treatment，and the patient´s health is improved after treatment.It is worthy of application.

To analyze the chemical composition of Yinyanghuo（Epimedii Folium）and its processed products and to investigate the proliferative and osteogenic effects of its extracts on mouse Embryonic Preosteoblast Cells（MC3T3-E1 cells）.

Ten batches of Yinyanghuo（Epimedii Folium）were processed into 10 batches of Yinyanghuo（Epimedii Folium）processed with lanolin oil and 10 batches of Yinyanghuo（Epimedii Folium）processed with salt，respectively.The contents of flavonoids in the samples were determined by high performance liquid chromatography（HPLC），and the changes of flavonoids in Yinyanghuo（Epimedii Folium）before and after processing were explored by HPLC fingerprint and multivariate statistical analysis.The osteogenic inhibition model of MC3T3-E1 cells induced by dexamethasone（DEX）was established.The anti-osteoporosis effect of Epimedium and its processed products were evaluated by measuring the expressions of alkaline phosphatase（ALP），osteoprotegerin（OPG）and osteocalcin（OCN）in cells，and the possible reasons for the different anti-osteoporosis effects of Yinyanghuo（Epimedii Folium）and its processed products were further explored.

The HPLC fingerprints of Yinyanghuo（Epimedii Folium）and its processed products were established，and the similarity of the fingerprints was evaluated，and 16 common peaks were identified.The results of cluster analysis classified epimedin A，epimedin B and epimedin C into one group，and icariin and baohuoside I into one group.The polar coordinate bubble diagram of each flavonoid content showed that the contents of primary flavonoid glycosides（epimedin A，epimedin B and epimedin C）decreased overall after processing，and the contents of secondary flavonoid glycosides（icariin and baohuoside I）increased overall after processing.The data of anti-osteoporosis activity showed that different processed products of Yinyanghuo（Epimedii Folium）had certain osteogenic proliferation effect，and the salt-processed Yinyanghuo（Epimedii Folium）had the most obvious effect on the enhancement of osteoblast activity inhibited by DEX.

Fingerprint similarity analysis and cluster analysis were used to analyze the differences in the contents of five flavonoids，so as to preliminarily explore the changes of flavonoids before and after processing. Combined with the effect of its extract on the proliferation and osteogenic ability of MC3T3-E1 cells，it was found that the content of icariin increased after salt processing of Yinyanghuo（Epimedii Folium），and the proliferation of osteogenic inhibition of MC3T3-E1 cells induced by DEX was significantly enhanced，suggesting that icariin may be the material basis for different anti-osteoporosis clinical effects of different processed Yinyanghuo（Epimedii Folium）products，laying a foundation for further exploration of the processing principle of Yinyanghuo（Epimedii Folium）.

To explore the clinical effect of Shugan Jieyu Xiaopi Prescription（疏肝解郁消痞方）in the treatment of functional dyspepsia（FD）of liver-stomach disharmony syndrome complicated with insomnia.

A total of 96 patients with FD of liver-stomach disharmony syndrome combined with insomnia admitted to the hospital from August 2021 to August 2023 were selected and divided into observation group（48 cases）and control group（48 cases）according to random number table method.The control group was given routine intervention，the observation group combined Shugan Jieyu Xiaopi Prescription.The course of treatment in both groups was 1 month.The clinical effect of the two groups was compared.Before treatment and 1 month after treatment，traditional Chinese medicine（TCM）syndrome scores，Nipin dyspepsia Symptom Index（NDSI），Nipin dyspepsia Life Quality Index（NDLQI），Pittsburgh Sleep Quality Index（PSQI），gastrointestinal hormones［motilin（MLT），gastrin（GAS），ghrelin］，neurotransmitters［5-hydroxytryptamine（5-HT），norepinephrine（NE）γ-aminobutyric acid（GABA）］and inflammatory factors［interleukin-6（IL-6），tumor necrosis factor-α（TNF-α）］were recorded and compared between the two groups.The occurrence of adverse reactions in the two groups was recorded.

The total effective rate of observation group was significantly higher than that of control group（P<0.05）.After treatment，the TCM syndrome scores of both groups were significantly decreased（P<0.05），and the score of the observation group was significantly lower than that of the control group（P<0.05）.After treatment，NDSI and PSQI scores of both groups were significantly decreased，and the scores of the observation group were significantly lower than those of the control group（P<0.05）.NDLQI scores of both groups were increased，and the score of the observation group was significantly higher than that of the control group（P<0.05）.After treatment，the levels of MLT and GASwere significantly decreased in both groups，and the levels of the observation group were significantly lower than those of the control group（P<0.05），the levels of ghrelin were increased in both groups，and the level of the observation group was significantly higher than that of the control group（P<0.05）.After treatment，the levels of NE in both groups were significantly decreased，and the level of the observation group was significantly lower than that of the control group（P<0.05）.The levels of 5-HT and GABA in both groups were increased，and the levels of the observation group were significantly higher than those of the control group（P<0.05）.After treatment，the levels of IL-6 and TNF-αwere significantly decreased in both groups，and the levels of the observation group were significantly lower than those of the control group（P<0.05）.There was no significant difference in the total incidence of adverse reactions between the two groups（P>0.05）.

Shugan Jieyu Xiaopi Prescription has a high efficacy in FD of liver-stomach disharmony syndrome complicated with insomnia.It can reduce the symptoms of patients，improve their sleep quality，gastrointestinal hormone and neurotransmitter levels，inhibit inflammation，and have a high safety.

To explore the effect of Xuanfei Huazhuo Formula（宣肺化浊方）on the expressions of CD86 and CD206 in alveolar lavage fluid of patients with sepsis lung injury with turbid toxin accumulation syndrome.

Sixty patients with sepsis induced lung injury（turbid toxin internal accumulation syndrome）who visited Hebei Provincial Hospital of Traditional Chinese Medicine from September 2022 to September 2023 were selected as the study subjects.The participants were randomly divided into an experimental group and a control group using a random coding table，with 30 cases in each group.The control group were given an equal amount of warm water as a control，while the experimental group were given nasal feeding of Xuanfei Huazhuo Formula on the basis of routine treatment.The total treatment period for both groups was 7 days.The 28-d mortality rate，clinical efficacy，severity of the condition，serum inflammatory response indicators，blood gas indicators，respiratory function indicators and expressions of CD86 and CD206 in alveolar macrophage phenotype in alveolar lavage fluid were compared between two groups.

Compared to the control group，the experimental group showed a higher overall effective rate and a lower 28-day mortality rate（P<0.05）.After treatment，the Acute Physiology and Chronic Health Evaluation（APACHE）Ⅱscore and lung injury score（LIS）of both groups decreased（P<0.05），and compared to the control group，the LIS score of the experimental group was lower（P<0.05）.After treatment，the levels of C-reactive protein（CRP），procalcitonin（PCT），interleukin-6（IL-6），tumor necrosis factor-α（TNF-α）and interleukin-10（IL-10）in both groups were reduced（P<0.05），and compared with the control group，the experimental group had lower levels of these indicators（P<0.05）.After treatment，the levels of arterial partial pressure of oxygen（PaO₂）and PaO₂/FiO₂ in both groups increased，while the levels of extravascular lung water index（EVLWI）and pulmonary vascular permeability index（PVPI）decreased（P<0.05）. Compared with the control group，the experimental group performed better on these indicators（P<0.05）.After treatment，the expression of CD68 increased and the expression of CD206 decreased in the alveolar lavage fluid of both groups（P<0.05），and compared to the control group，the experimental group had higher expression of CD68 and lower expression of CD206（P<0.05）.

Xuanfei Huazhuo Formula has definite therapeutic effect in treating patients with sepsis induced lung injury due to the accumulation of turbid toxin.Its mechanism of action may be closely related to the regulation of CD86 and CD206 in alveolar lavage fluid，and has clinical application and promotion value.

To observe the effects of Modified Buzhong Yiqi Huoxue Decoction（加味补中益气活血汤）combined with consciousness-restoring and orifice-opening acupuncture and old ten needles on the nutritional status and neurological function of the elderly patients with cerebral infarction of Qi deficiency and blood stasis syndrome.

One hundred and twenty elderly patients with cerebral infarction with Qi deficiency and blood stasis syndrome were randomly divided into the conventional group（routine treatment+Modified Buzhong Yiqi Huoxue Decoction+routine acupuncture）and the experimental group（routine treatment+Modified Buzhong Yiqi Huoxue Decoction+consciousness-restoring and orifice-opening acupuncture and old ten needles），with 60 cases in each group.The TCM syndrome scores，neurological function，hemorheological indexes and nutritional status before and after treatment were compared between the two groups.The clinical efficacy and adverse reactions were also compared.

After treatment，the TCM main symptom scores，secondary symptom scores，total score，National Institutes of Health Stroke Scale（NIHSS）score，oxidized low-density lipoprotein（ox-LDL），neuronal nuclear antigen（NeuN），whole blood viscosity（BV），whole blood reduced viscosity（BRV）and plasma viscosity（PV）in both groups were lower than those before treatment（P<0.05），and those in the experimental group were lower than those in the conventional group（P<0.05）.After treatment，the Mini Nutritional Assessment（MNA）score，the levels of albumin（ALB）and prealbumin（PA）in both groups were higher than those before treatment（P<0.05），and those in the experimental group were higher than those in the conventional group（P<0.05）.The distribution of clinical efficacy grades and the total effective rate in the experimental group were higher than those in the conventional group（P<0.05）.There were no obvious adverse reactions in the conventional group，and one case of redness and swelling at the acupuncture site in the experimental group，which relieved without intervention.

Modified Buzhong Yiqi Huoxue Decoction combined with consciousness-restoring and orifice-opening acupuncture and old ten needles can improve the TCM syndrome，neurological function，hemorheological indexes and nutritional status of the elderly patients with cerebral infarction of Qi deficiency and blood stasis syndrome and enhance the clinical efficacy，with high safety.

To study the therapeutic effect of Jiawei Diyu Powder（加味地榆散）on lung cancer targeted therapy in patients with oral mucositis of Yin deficiency and heat toxin syndrome.

86 patients with lung cancer targeted therapy for oral mucositis with Yin deficiency and heat toxin syndrome were randomly divided into two groups according to the order of treatment.There were 43 cases in each group.The patients in the control group were treated with Compound Chlorhexidine Gargle and oral administration of vitamin B₂ and vitamin C，and those in the observation group were treated with Jiawei Diyu Powder on the basis of the control group.The oral mucositis grading，TCM symptom scores（dry mouth and throat，sore mouth and tongue，swollen gums and pain，hand-foot-heart heat），inflammatory factor levels［serum interleukin-6（IL-6），interleukin-8（IL-8）and tumor necrosis factor（TNF-α）］，Karnofsky performance score（KPS）and adverse reactions（abnormal taste，yellow tongue coating，nausea and vomiting，belching，etc.）were compared between the two groups.

There was no significant difference in the classification of oral mucositis between the two groups before treatment（P>0.05）.After treatment，the observation group was significantly better than the control group（P<0.05）.After treatment，the above scores of the two groups were significantly decreased（P<0.05），and the scores of TCM symptoms in the observation group were significantly lower than those in the control group（P<0.05）.There was no significant difference in serum IL-6，IL-8 or TNF-αbetween the two groups before treatment（P>0.05）.After treatment，the levels of inflammatory factors in the two groups were significantly decreased（P<0.05），and the levels of the observation group were significantly lower than those of the control group（P<0.05）.After treatment，the KPS scores of the two groups were significantly increased，and the score of the observation group was significantly higher than that of the control group（P<0.05）.The incidence of adverse reactions in the observation group and the control group was 9.30%（4/43）and 6.98%（3/43），respectively，and the difference was not statistically significant（P>0.05）.

Jiawei Diyu Powder has a satisfactory therapeutic effect on patients with lung cancer targeted treatment of oral mucositis with Yin deficiency and heat toxin syndrome.It can significantly improve the classification of oral mucositis，eliminate the inflammation，relieve various discomfort symptoms，and improve the health level of the body.It is a safe and effective medication regimen.

To investigate the effects of Baishao（Radix Paeoniae Alba）on rats with irritable bowel syndrome with diarrhea（IBS-D）and irritable bowel syndrome with constipation（IBS-C）.

Sixty-six SD male rats aged 4 weeks were randomly divided into normal control group，IBS-D model group，IBS-D drug group［high，medium and low doses of Baishao（Radix Paeoniae Alba）and pivilium bromide］，IBS-Cmodel group and IBS-C drug group［high，medium and low doses of Baishao（Radix Paeoniae Alba）and moxapride］.Acetic acid stimulation combined with binding stress was used for IBSD modeling，and ice water gavage combined with binding stress was used for IBS-C modeling.The high，medium and low dose groups were administrated with Baishao（Radix Paeoniae Alba）（7.2，3.6，1.8 g/kg body mass，respectively），piverium bromide aqueous solution（13.5 mg/kg）and moxabi aqueous solution（1.35 mg/kg）for 2 weeks，respectively.The weight was performed，the stool was collected，the abdominal withdrawal reflex（AWR）score was performed.The expressions of vasoactive intestinal peptide（VIP）and nuclear transcription factorκB（NF-κB）in ileocecal colon were detected by immunohistochemistry and ELISA.The expression of protease activating receptor 2（PAR2）in intestinal tissues was detected by Western blot.

（1）IBS-D：Baishao（Radix Paeoniae Alba）at high，medium and low doses and piveronium bromide could improve visceral hypersensitivity in model rats（P<0.05，P<0.01，P<0.01，P<0.01，respectively）.Baishao（Radix Paeoniae Alba）at middle and low doses and piveronium bromide could reduce the fecal water content of model rats（P<0.01），but Baishao（Radix Paeoniae Alba）at high dose had no significant effect on reducing fecal water content of model rats（P>0.05）.The weight gain of model group was lower than that of the normal control group（P<0.01），and Baishao（Radix Paeoniae Alba）at high dose could reduce the weight gain of the model group（P<0.05）.Baishao（Radix Paeoniae Alba）at high dose could decrease the expression of NF-κB in model rats（P<0.01）.Baishao（Radix Paeoniae Alba）at high and medium doses could decrease the expression of PAR2 in model mice（P<0.01，P<0.05）.（2）IBS-C：Baishao（Radix Paeoniae Alba）at medium and low doses could improve visceral hypersensitivity of model rats（all P<0.05）.Baishao（Radix Paeoniae Alba）at high dose could increase fecal water content of model rats（P<0.01）.The weight gain of the model group was lower than that of the normal control group（P<0.05），and the drug group had no effect on the body weight of the model rats（all P>0.05）.Baishao（Radix Paeoniae Alba）at high dose and mosapride could decrease VIP expression in model rats（P<0.05，P<0.01）.Baishao（Radix Paeoniae Alba）at low dose could decrease PAR2 expression in IBS-Cmodel rats（P<0.05）.

Baishao（Radix Paeoniae Alba）can improve the visceral hypersensitivity of the rats with IBS-D and IBS-C at the same time，which may be related to the decrease of PAR2 expression.Medium and low doses of Baishao（Radix Paeoniae Alba）can alleviate diarrhea of the rats with IBS-D，while high doses of Baishao（Radix Paeoniae Alba）can promote defecation of the rats with IBS-C，which may be related to the difference of multi-target effects of Baishao（Radix Paeoniae Alba）on different models.

To analyze the effect of Buyi Jianpi Recipe（补益健脾方）on hormone，plasma fibrinogen and prognosis of postpartum lochia with Qi deficiency and blood stasis.

Ninety-six cases of postpartum lochia with Qi deficiency and blood stasis syndrome were randomly divided into the study group and the control group，with 48 cases in each group.Both groups were treated with hemostasis，anti-infection and uterine contraction，while the study group was treated with Buyi Jianpi Recipe for 2 weeks.The changes of TCM syndrome scores，serum hormones，plasma fibrinogen and uterine artery hemodynamics were compared between the two groups before and after treatment，and the therapeutic effect and safety of the two groups were evaluated.

After treatment，the scores of postpartum lochia，tongue picture，pulse and abdominal pain in both groups decreased compared with those before treatment.After treatment，the scores of postpartum lochia，tongue picture，pulse and abdominal pain in the study group were lower than those in the control group with statistical significance（P<0.05）.After treatment，the levels of progesterone（P）andβ-human chorionic gonadotropin（β-HCG）decreased and the level of estrogen（E₂）increased in both groups.After treatment，the levels of P andβ-hCG in the study group were lower than those in the control group，and the level of E₂ was higher than that in the control group with statistical significance（P<0.05）.The levels of fibrinogen（FIB）and D-dimer（D-D）in both groups decreased after treatment.The levels of FIB and D-D in the study group were lower than those in the control group after treatment，and the difference was statistically significant（P<0.05）.After treatment，the values of resistance index（RI）and pulse index（PI）in both groups decreased，and the values of end diastolic blood flow velocity（EDV）and peak systolic velocity（PSV）increased.After treatment，the values of RI and PI of the study group were lower than those of the control group，and the values of EDV and PSV of the study group were higher than those of the control group with statistical significance（P<0.05）.The total effective rate of the study group after treatment was93.75%（45/48），which was higher than that of the control group（79.17%，38/48），and the difference was statistically significant（P<0.05）.No obvious adverse reactions occurred in both groups during treatment.

For the treatment of postpartum lochia with Qi deficiency and blood stasis，Buyi Jianpi Recipe can not only improve the hormone level，coagulation and fibrinolysis function，and uterine artery hemodynamics，but also alleviate the clinical symptoms and improve the prognosis on the premise of ensuring safety.

To investigate the effects of Huangqi Guizhi Wuwu Decoction（黄芪桂枝五物汤）on microRNA-146a（miR-146a），myeloid differentiation factor 88（MyD88）/inhibitor of nuclear factor-κB inhibitory protein（IκB）signaling pathway in diabetic peripheral neuropathy（DPN）patients.

A total of 108 DPN patients were selected and divided into treatment group and control group by random number table method，with 54 cases in each group.The control group was treated with mecobalamine and vitamin B₁，and the treatment group was treated with Huangqi Guizhi Wuwu Decoction.The course of treatment in both groups was 3 months.The clinical efficacy of the two groups was evaluated.Traditional Chinese medicine（TCM）syndrome score，sensory nerve conduction velocity（SNCV），motor nerve conduction velocity（MNCV），Toronto Clinical Scoring System（TCSS）score，the levels of miR-146a，MyD88 protein，IκBαprotein and phosphorylated-IκBα（p-IκBα）protein before and after treatment and the occurrence of adverse reactions were compared between the two groups.

The total effective rate of the treatment group was 92.59%（50/54），which was significantly better than 77.78%（42/54）of the control group（P<0.05）.After treatment，the scores of TCM syndrome in both groups were improved compared with those before treatment（P<0.05），and the treatment group was better than the control group（P<0.05）.After treatment，the total knee SNCV，median SNCV，total knee MNCV，median MNCV and TCSS scores of both groups were improved compared with those before treatment（P<0.05），and the treatment group was better than the control group（P<0.05）.After treatment，the levels of miR-146a，MyD88 protein，IκBαprotein and p-IκBαprotein in the treatment group were improved compared with those before treatment（P<0.05），and the treatment group was better than the control group（P<0.05）.There was no difference in the incidence of adverse reactions between the two groups（P>0.05）.

Huangqi Guizhi Wuwu Decoction has good efficacy in DPN.It can effectively reduce clinical symptoms，improve nerve conduction velocity，inhibit the expressions of miR-146a and MyD88/IκB signaling pathway，and has high safety.

To explore the mechanism of Yangxue Qingnao Granules（养血清脑颗粒）combined with Qiangli Dingxuan Tablets（强力定眩片）in treating dizziness and headache caused by hypertension.

A total of 200 patients with hypertension，dizziness and headache were selected.The participants were randomly divided into an experimental group and a control group using a random coding table，with 100 patients in each group.The control group received routine treatment，while the experimental group received Yangxue Qingnao Granules combined with Qiangli Dingxuan Tablets on the basis of the control group.Both groups were treated continuously for 4 weeks.The cognitive function，clinical efficacy，blood pressure control，improvement of dizziness and headache symptoms，serum levels of C-reactive protein（CRP），homocysteine（Hcy），vascular endothelial growth factor（VEGF），arterial blood flow velocity and complications were compared between the two groups.

After treatment，the MMSE and MoCA scores of the two groups increased（P<0.05）. Compared with the control group，the experimental group had higher MMSE and MoCA scores（P<0.05）. Compared with that of the control group，the total effective rate of the experimental group was higher（P<0.05）.The scores of the quality of life of two groups increased（P<0.05）. Compared with that of the control group，the score of the quality of life of the experimental group was higher（P<0.05）.The systolic and diastolic blood pressures of two groups decreased（P<0.05）. Compared with the control group，the experimental group had lower systolic and diastolic blood pressure，and shorter improvement time for dizziness and headache（P<0.05）.The levels of CRP and Hcy decreased and VEGF levels increased in both groups（P<0.05）. Compared with the control group，the experimental group had lower levels of CRP and Hcy，and higher VEGF levels（P<0.05）.The average blood flow velocity of left vertebral artery（LVA），right vertebral artery（RVA）and basilar artery（BA）in the two groups increased（P<0.05）. Compared with those of the control group，the average blood flow velocity of LVA，RVA and BA in the experimental group was faster（P<0.05）. Compared with the control group，there was no significant difference in the incidence of complications in the experimental group（P>0.05）.

The combination of Yangxue Qingnao Granules and Qiangli Dingxuan Tablets can effectively improve the effect of blood pressure control，improve the dizziness and headache symptoms caused by hypertension，and improve the quality of life of patients.The mechanism may be closely related to the regulation of CRP，Hcy and VEGF levels and arterial blood flow velocity，which is suitable for clinical application and promotion.

To investigate the effect of Yangyin Tongbi Jiejing Decoction（养阴通痹解痉汤）combined with functional training on hemiplegia after stroke.

A total of 108 patients with hemiplegia after stroke in the hospital were randomly divided into two groups with 54 patients in each group.The control group was given functional training，including regular turning over，positioning of the affected limb，sitting and sitting balance，sitting and standing transfer training，standing balance training，etc.The treatment group was given Yangyin Tongbi Jiezhu Decoction（1 dose/d）on the basis of the control group.The treatment time was 4 weeks.The therapeutic effect，TCM syndrome scores，Fugl-Meyer assessment scale（FMA），Barthel index（BI）Stroke-Specific Quality of Life Scale（SS-QOL），hemiplegic side knee joint，lower limb hip joint extension muscle strength，ankle dorsiflexion muscle strength，serum inflammatory factors and hemorheology index levels were compared after treatment.

After treatment，the total effective rate of the treatment group was higher（P<0.05）.After treatment，the Fugl-Meyer score，BI score and SS-QOL score，the extension muscle strength of hemiplegic knee joint，lower limb hip joint and the dorsiflexion muscle strength of ankle joint in both groups were significantly higher than those before treatment（P<0.05）.And these indexes of the treatment group increased more obviously（P<0.05）.After treatment，the levels of hematocrit（Hct），plasma fibrinogen（FIB）and plasma viscosity（PV），serum tumor necrosis factor alpha（TNF-α），interleukin6（IL-6）and hypersensitive C-reactive protein（hs-CRP），and TCM syndrome scores in both groups were significantly lower than those before treatment（P<0.05），and the decrease was significant in the treatment group（P<0.05）.

Yangyin Tongbi Jiejing Decoction combined with functional training has a good effect on patients with hemiplegia after stroke.

To analyze the effect of consciousness-restoring and orifice-opening acupuncture combined with brain-computer interface therapy on upper limb function in patients with spastic upper limb hemiplegia after cerebral infarction.

63 patients with upper limb spastic hemiplegia after cerebral infarction were divided into the control group（32 cases）and the combined group（31 cases）by random number table method.The control group was treated with brain-computer interface，and the combined group was treated with brain-computer interface combined with consciousness-restoring and orifice-opening acupuncture.Both groups were treated for 3 weeks.The neurological function deficit，activities of daily living，upper limb function，electrophysiology，vascular endothelial function of two groups before and after 3 weeks of treatment and the safety during treatment were compared.

Compared with those before treatment，after 3 weeks of treatment，the National institutes of health stroke scale（NIHSS）score，the serum levels of C-reactive protein（CRP）and endothelin（ET）in the two groups decreased，and the scores and levels were lower than those in the combined group（P<0.05）. Compared with those before treatment，after 3 weeks of treatment，the scores of Barthel index（BI），Fugl-meyer Motor function（FMA），Action research arm test（ARAT），WOLF motor function evaluation scale（WMFT）and serum nitric oxide（NO）levels increased in both groups，and those were higher in the combined group（P<0.05）. Compared with those before treatment，the motor evoked potential latency（MEP）and central motor conduction time（CMCT）of the two groups were shortened after 3 weeks of treatment，and the time of the combined group was shorter（P<0.05）.During treatment，the adverse reactions did not affect the course of treatment in both groups，and the incidence of adverse reactions was similar［16.13%（5/31）vs 9.38%（3/32），P>0.05］.

consciousness-restoring and orifice-opening acupuncture combined with brain-computer interface therapy could improve the upper limb function，reduce the nerve function defect and improve the ability of daily living in patients with upper limb spastic hemiplegia after cerebral infarction，which was analyzed that it is related to the regulation of electrophysiology and vascular endothelial function，and had good safety.

To investigate the clinical effect of Yinhua Qingdu Decoction（银花清毒汤）combined with acupuncture on dry eye caused by meibomian gland dysfunction（MGD）.

A total of 100 patients with MGD-induced dry eye admitted from January 2021 to December 2023 were included in this grouping trial.The patients were divided into Western medicine group（49 cases）and integrated traditional Chinese and Western medicine group（51 cases）by random number table method.All patients in the western medicine group were treated with metaromian gland massage and artificial tears.The patients in the integrated traditional Chinese and western medicine group were treated with meibomian gland massage and artificial tears，combined with the treatment of Yinhua Qingdu Decoction and acupuncture.The data of patients in each group was observed，including clinical treatment outcome，the changes in ocular function indexes（tear film rupture time and tear secretion，corneal fluorescence staining score），subjective dry eye symptom score，and ocular surface disease index before and after treatment，the ocular surface disease index（OSDI）score changes，the levesl of interleukin-10（IL-10）and interleukin-13（IL-13），the change of scores of National Eye Institute 25-Item Visual Function Questionnaire（NEI-VFQ-25）and adverse reactions.

The total effective rate of the integrated Chinese and western medicine group was significantly higher than that of Western medicine group（P<0.05）.Before treatment，the ocular function indexes（tear film rupture time and tear secretion volume，corneal fluorescence staining score），subjective dry eye symptom score，OSDI score，the levels of IL-10 and IL-13 and NEI-VFQ-25 score were not significant（P>0.05）.After treatment，the ocular function indexes（tear film rupture time and tear secretion volume，corneal fluorescence staining score），dry eye subjective symptom score，OSDI score，IL-10 and IL-13 levels，and NEI-VFQ-25 score were improved in all groups.After treatment，the ocular function indexes（tear film rupture time，tear secretion，corneal fluorescence staining score），subjective dry eye symptom score，OSDI score，the levels of IL-10 and IL-13 and NEI-VFQ-25 score in the integrated traditional Chinese and Western medicine group were improved better than those in Western medicine group（P<0.05）.The rate of adverse reaction was higher in both groups（P>0.05）.

The combination of Yinhua Qingdu Decoction and acupuncture in the treatment of dry eye caused by MGD has significant clinical effect，which can effectively reduce the inflammatory response of patients，improve the symptoms，eye function and visual quality of patients，and reduce the adverse reaction of treatment，and the treatment is safe and feasible.

To investigate the effects of Xiaoqinglong Decoction（小青龙汤）combined with glucocorticoids on pulmonary function and serum indexes in patients with bronchial asthma.

A total of 108 patients with bronchial asthma were selected and divided into two groups according to random number table method.The study group（54 cases）was treated with Xiaoqinglong Decoction combined with glucocorticoid，and the control group（54 cases）was treated with glucocorticoid.The clinical efficacy，TCM syndrome scores，lung function indexes［forced expiratory volume in first second（FEV1），forced vital capacity（FVC），forced expiratory flow at 50% of FVC exhaled（FEF50%），peak expiratory flow rate（PEF）］，laboratory indexes［C-reactive protein（CRP），serum transforming growth faction-β1（TGF-β1），Cellular signal transduction molecules-3（Smad-3）］and adverse reactions of the two groups were compared.

The total effective rate of the study group was higher than that of the control group（P<0.05）.After treatment，the scores of cough，wheezing，sputum and headache in both groups were decreased（P<0.05），and the scores in the study group were lower than those in the control group（P<0.05）.After treatment，the values of FEV1，FVC，FEF50% and PEF of the study group were increased（P<0.05），and the values of the study group were higher than those of the control group（P<0.05）.After treatment，the levels of CRP，TGF-β1 and SAMad-3 in both groups were decreased（P<0.05），and the levels of in the study group were lower than those in the control group（P<0.05）.There was no significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Xiaoqinglong Decoction combined with glucocorticoid therapy in patients with bronchial asthma can improve lung function indexes andreduce the levels of TGF-β1 and SAMAD-3，with good efficacy and safety.

To explore the therapeutic effect of Xiaoer Feire Kechuan Granules（小儿肺热咳喘颗粒）combined with vitamin D in the treatment of mycoplasma pneumoniae pneumonia in children.

A total of 302 children with mycoplasma pneumoniae pneumonia（admitted from October 2021 to December 2022）were included in the study and divided into control group and observation group by random number table method，with 151 cases in each group.The children in the control group received conventional Western medicine treatment and supplemented with vitamin D treatment.Those in the observation group were treated with Xiaoer Feire Kechuan Granules on the basis of medication in the control group.During the treatment and study，9 cases fell off in the control group and 10 cases fell off in the observation group.The clinical data of children in each group was compared，including the clinical efficacy，the changes in TCM symptom scores（fever，chest tightness and distension，cough and asthma，yellow phlegm not easy to cough up，body heat sweating，etc.）before and after treatment，time for fever to disappear，time for cough to disappear，time for sputum relief，time for lung moist rale to disappear，changes in lung function indicators，inflammation and immune-related indicators，and adverse reactions.

The clinical effect of the observation group was better than that of the control group（P<0.05）. Compared with that of the control group，the disappearance time of fever，cough，sputum relief and pulmonary moist rales was shorter in the observation group（P<0.05）.After treatment，TCM symptom scores（fever，chest tightness and distension，cough and asthma，yellow phlegm not easy to cough up，body heat sweating，etc.）in two groups were lower.The values of forced vital capacity（FVC），forced expiratory volume in one second（FEV1），FEV1/FVC and other pulmonary function indicators of two groups were significantly increased.The levels of C-reactive protein，CRPand procalcitonin（PCT）and other inflammatory indicators were decreased.The levels of immunoglobulin A（IgA），immunoglobulin G（IgG），immunoglobulin M（IgM）and other levels were all increased，and the scores of TCM symptoms（fever，chest tightness and distension，cough and asthma，yellow phlegm not easy to cough up，body heat and sweat，etc.）and inflammatory indicators（PCT and CRP）in the observation group were lower than those in the control group after treatment.The pulmonary function indexes（FVC，FEV1，FEV1/FVC）and immune indexes（IgA，IgG，IgM）were higher than those of the control group（P<0.05）.The adverse reaction rate of the observation group was lower than that of the control group（P<0.05）.

Xiaoer Feire Kechuan Granules combined with vitamin D has good curative effect in the treatment of mycoplasma pneumoniae pneumonia in children，with alleviated symptoms，rapid recovery，reduced inflammation，improved immune function and lung function and less adverse reactions，and the treatment is safe and reliable.