Home Journals Articles Updates About
CN
image
收藏切换
Mechanism of Electroacupuncture Inhibition of Muscle Cell Apoptosis in Rats with Fascial Pain Syndrome Model Based on BDNF/Trkb/p38/JNK Signal Pathway
收藏切换
Lie WANG1, 2, Tieming MA1, 2, Jiaxiang YU1, 2, Xingxing LIN1, 2, Shudong WANG1, 2, Junjie MA1, 2, Jiquan LI1, 2, Gege LI1, 2, Di BIAN1, 2, Ying WANG1, 2, Zhe HU1, 2, Shuai MA3, Baoqiang DONG1, 2
Chinese Archives of Traditional Chinese Medicine | 2025, 43(8) : 44 - 50
Less
收藏切换
Chinese Archives of Traditional Chinese Medicine | 2025, 43(8): 44-50
• Target on National Project •
Mechanism of Electroacupuncture Inhibition of Muscle Cell Apoptosis in Rats with Fascial Pain Syndrome Model Based on BDNF/Trkb/p38/JNK Signal Pathway
Full
Lie WANG1, 2, Tieming MA1, 2, Jiaxiang YU1, 2, Xingxing LIN1, 2, Shudong WANG1, 2, Junjie MA1, 2, Jiquan LI1, 2, Gege LI1, 2, Di BIAN1, 2, Ying WANG1, 2, Zhe HU1, 2, Shuai MA3, Baoqiang DONG1, 2
Affiliations
  • 1.Liaoning University of Traditional Chinese Medicine，Shenyang 110847，Liaoning，China
  • 2.Key Laboratory of Acupuncture Moxibustion and Health Rehabilitation in Liaoning Province，Shenyang 110847，Liaoning，China
  • 3.Shenyang Seventh People´s Hospital，Shenyang 110003，Liaoning，China
Published: 2025-08-10 doi: 10.13193/j.issn.1673-7717.2025.08.011
Outline
收藏切换
Objective

Based on the “brain-derived neurotrophic factor（BDNF）/phospho-tropomyosin receptor kinase B（TrkB）/p38/c-Jun N-terminal kinase（JNK）”signaling pathway，this study aimed to investigate the mechanism of electroacupuncture stimulation of pain points in the treatment of myofascial pain syndrome（MPS）.

Method

SD rats were divided into normal group，model group，electroacupuncture group and western medicine group.MPSmodel rats were established by a combination of percussion and centrifugal exercise，followed by electroacupuncture stimulation of pain points and oral administration of celecoxib for intervention.After the experiment，the morphology of muscle tissues in rats of each group was observed by hematoxylin-eosin（HE）and Masson staining.Prussian blue staining was used to observe the degranulation of mast cells in the muscle tissues of each group.TUNEL fluorescence staining was used to observe the cell apoptosis in the muscle tissues of each group.Reverse Transcription Polymerase Chain Reaction（RT-PCR）was used to detect the mRNA expression levels of Bcl-2 associated X protein（Bax），B-cell lymphoma-2（Bcl-2），cysteine aspartate-specific protease 3（Caspase-3），BDNF，TrkB，p38 and JNK in the muscle tissues of each group.Wes was used to detect the protein expression levels of Bax，Bcl-2，Caspase-3，BDNF，p-TrkB，p-p38 and p-JNK in the muscle tissues of each group.

Result

HE and Masson staining indicated that both electroacupuncture and celecoxib intervention can improve muscle pathological damage caused by MPS，and can inhibit mast cells degranulation and apoptosis of muscle cell.Electroacupuncture and celecoxib intervention can increase Bcl-2 mRNA expression and decrease the mRNA expressions of Bax，Caspase-3，BDNF，TrkB，p38 and JNK，increase Bcl-2 protein expression and decrease protein expressions of Bax，Caspase-3，BDNF，p-TrkB，p-p38 and p-JNK.Electroacupuncture was more effective than celecoxib.

Conclusion

Electroacupuncture can treat MPS by inhibiting the BDNF/Trkb/p38/JNK signaling pathway，thereby reducing cell apoptosis in MPSaffected muscle tissue.In traditional Chinese medicine theory，electroacupuncture can be used to restore the imbalance of Qi，blood，Yin and Yang in the body，but the corresponding mechanism still needs further exploration.

BDNF/Trkb/p38/JNK signal pathway  /  myofascial pain syndrome  /  electroacupuncture  /  cell apoptosis
王列, 马铁明, 于嘉祥, 林星星, 王树东, 马俊杰, 李记泉, 李格格, 卞镝, 王鹰, 胡哲, 马帅, 董宝强. 基于BDNF/Trkb/p38/JNK信号通路研究电针抑制筋膜疼痛综合征模型大鼠肌肉细胞凋亡的机制. 中华中医药学刊, 2025 , 43 (8) : 44 -50 . DOI: 10.13193/j.issn.1673-7717.2025.08.011
Lie WANG, Tieming MA, Jiaxiang YU, Xingxing LIN, Shudong WANG, Junjie MA, Jiquan LI, Gege LI, Di BIAN, Ying WANG, Zhe HU, Shuai MA, Baoqiang DONG. Mechanism of Electroacupuncture Inhibition of Muscle Cell Apoptosis in Rats with Fascial Pain Syndrome Model Based on BDNF/Trkb/p38/JNK Signal Pathway[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (8) : 44 -50 . DOI: 10.13193/j.issn.1673-7717.2025.08.011
Appendix
Less
Year 2025 volume 43 Issue 8
44
20
Cite this Article
BibTeX
Article Info
doi: 10.13193/j.issn.1673-7717.2025.08.011
  • Online Date：2026-10-30
  • Published：2025-08-10
Article Data
Affiliations
History
Funding
Affiliations
    1.Liaoning University of Traditional Chinese Medicine，Shenyang 110847，Liaoning，China
    2.Key Laboratory of Acupuncture Moxibustion and Health Rehabilitation in Liaoning Province，Shenyang 110847，Liaoning，China
    3.Shenyang Seventh People´s Hospital，Shenyang 110003，Liaoning，China
References
Share
https://castjournals.cast.org.cn/joweb/zhzyyxk/EN/10.13193/j.issn.1673-7717.2025.08.011
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House