Home Journals Articles Updates About
CN
image
收藏切换
Research on Role of LncRNA CASC9/miR-195-5p/E2F7 Signaling Pathway in Dihydromyricetin Inhibiting Proliferation Andmetastasis of Esophageal Squamous Cell Carcinoma
收藏切换
Xuan ZHENG1, Lanxiang HUANG2, Yishuang CUI1, Yanan WU1, Ziqian HONG1, Mengshi ZHANG1, Guogui SUN2
Chinese Archives of Traditional Chinese Medicine | 2025, 43(8) : 65 - 70
Less
收藏切换
Chinese Archives of Traditional Chinese Medicine | 2025, 43(8): 65-70
• Target on National Project •
Research on Role of LncRNA CASC9/miR-195-5p/E2F7 Signaling Pathway in Dihydromyricetin Inhibiting Proliferation Andmetastasis of Esophageal Squamous Cell Carcinoma
Full
Xuan ZHENG1, Lanxiang HUANG2, Yishuang CUI1, Yanan WU1, Ziqian HONG1, Mengshi ZHANG1, Guogui SUN2
Affiliations
  • 1.School of Public Health，North China University of Science and Technology，Tangshan 063200，Hebei，China
  • 2.North China University of Science and Technology Affiliated Hospital，Tangshan 063000，Hebei，China
Published: 2025-08-10 doi: 10.13193/j.issn.1673-7717.2025.08.015
Outline
收藏切换
Objective

To investigate the effect of dihydromyricetin（DMY）on proliferation and metastasis of esophageal squamous cell carcinoma（ESCC）and whether it is mediated by the LncRNA CASC9/miR-195-5p/E2F7 axis.

Methods

Gradient concentrations（0，25，50，100μmol/L）of DMY were used to treat ESCC cells in order to investigate the effects of DMY on ESCC cells proliferation，migration and invasion.The possible interaction between the LncRNA CASC9/miR-195-5p/E2F7 axis in ESCC was predicted using bioinformatics.Real-time quantitative polymerase chain reaction（RT-qPCR）assay was used to detect the expressions of LncRNA CASC9，miR-195-5p and E2F7 in cells，and the regulation relationship was confirmed using RT-qPCR，Western blot and double luciferase reporter gene assays.In order to test the cell viability，proliferation，migration and invasion ability，cell counting kit-8（CCK-8），colony formation，wound healing and Transwell invasion assays were used respectively.Western blot assay was used to test the expressions of epithelial mesenchymal transition（EMT）markers E-cadherin，NCadherin and Vimentin.

Results

The proliferation，migration and invasion abilities of ESCC cells were significantly inhibited by DMY.LncRNA CASC9 was hyperexpressed in ESCC by bioinformatics analysis and cell level verification.The proliferation，migration and invasion abilities of ESCC cells were inhibited by DMY treatment leaded to by down-regulating the expression level of LncRNA CASC9 in ESCC cells.Furthermore，the miR-195-5p expression was down-regulated in ESCC while E2F7 expression was up-regulated.RT-qPCR，Western blot and double luciferase reporter gene assays all confirmed that LncRNA CASC9 regulated E2F7 expression by adsorbing miR-195-5p.E2F7 knocking down-reversed the promotion of miR-195-5p knocking down on the proliferation，metastasis and EMT process of ESCC，whereas E2F7 overexpression reversed the inhibition of miR-195-5p overexpression.

Conclusion

DMY inhibits ESCC proliferation and metastasis via the LncRNA CASC9/miR-195-5p/E2F7 axis.

dihydromyricetin  /  LncRNA CASC9  /  miR-195-5p  /  E2F7  /  esophageal squamous cell carcinoma  /  metastasis
郑璇, 黄兰香, 崔逸爽, 吴亚男, 洪紫谦, 张梦诗, 孙国贵. LncRNA CASC9/miR-195-5p/E2F7信号通路在二氢杨梅素抑制食管鳞状细胞癌增殖和转移中的作用研究. 中华中医药学刊, 2025 , 43 (8) : 65 -70 . DOI: 10.13193/j.issn.1673-7717.2025.08.015
Xuan ZHENG, Lanxiang HUANG, Yishuang CUI, Yanan WU, Ziqian HONG, Mengshi ZHANG, Guogui SUN. Research on Role of LncRNA CASC9/miR-195-5p/E2F7 Signaling Pathway in Dihydromyricetin Inhibiting Proliferation Andmetastasis of Esophageal Squamous Cell Carcinoma[J]. Chinese Archives of Traditional Chinese Medicine, 2025 , 43 (8) : 65 -70 . DOI: 10.13193/j.issn.1673-7717.2025.08.015
Appendix
Less
Year 2025 volume 43 Issue 8
35
14
Cite this Article
BibTeX
Article Info
doi: 10.13193/j.issn.1673-7717.2025.08.015
  • Online Date：2026-10-30
  • Published：2025-08-10
Article Data
Affiliations
History
Funding
Affiliations
    1.School of Public Health，North China University of Science and Technology，Tangshan 063200，Hebei，China
    2.North China University of Science and Technology Affiliated Hospital，Tangshan 063000，Hebei，China
References
Share
https://castjournals.cast.org.cn/joweb/zhzyyxk/EN/10.13193/j.issn.1673-7717.2025.08.015
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House