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Outline
To investigate the effect of shikonin on the cell proliferation，migration，invasion and apoptosis of nasopharyngeal carcinoma（NPC）cells by regulating miR-224 expression.
Thirty NPC patients treated in the hospital from January 2023 to March 2024 were selected and underwent surgery.The adjacent and cancerous tissues were removed during the surgery.The normal cultured human SUNE-1 cells were recorded as the blank control group，those treated with shikonin were designated as the shikonin group.Those treated with shikonin and transfected with miR-NC and miR-224 mimetics were designated as the miR-NC and miR-224 groups.The expression of miR-224 in each group was compared，and the effects of shikonin on cell proliferation，migration，invasion and apoptosis were observed by regulating miR-224 expression.
The expression of miR-224 in NPC tissue was（3.23±0.16），which was higher than that（1.09±0.07）in adjacent tissues（P<0.05）. Comparison of miR-224 expression among the groups：shikonin+miR-224 group>blank control group>shikonin+miR-NC group>shikonin group，and the pairwise comparisons among groups were statistically significant（P<0.05）. Comparison of optical density（OD）value，number of migrating cells and number of invading cells among the groups：blank control group>miR-224 group>miR-NC group>shikonin group. Comparison of apoptosis rate among the groups：blank control group<miR-224 group<miR-NC group<shikonin group.There were statistically significant pairwise comparisons among groups（P<0.05）. Comparison of CyclinD1 protein，matrix metalloproteinase2（MMP-2）protein，MMP-9 protein and B-cell lymphoma-2（Bcl-2）protein among the groups：blank control group>miR-224 group>miR-NC group>shikonin group. Comparison of cyclin-dependent kinase inhibitor 1A（p21）protein and Bcl-2 associated X protein（Bax）protein among the groups：blank control group<miR-224 group<miR-NC group<shikonin group.There were statistically significant pairwise comparisons among groups（P<0.05）.
Shikonin can reduce the expression of miR-224 in NPC patients，inhibit the cell proliferation，migration and invasion，and induce apoptosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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