Full
Outline
To analyze the chemical composition of Yinyanghuo（Epimedii Folium）and its processed products and to investigate the proliferative and osteogenic effects of its extracts on mouse Embryonic Preosteoblast Cells（MC3T3-E1 cells）.
Ten batches of Yinyanghuo（Epimedii Folium）were processed into 10 batches of Yinyanghuo（Epimedii Folium）processed with lanolin oil and 10 batches of Yinyanghuo（Epimedii Folium）processed with salt，respectively.The contents of flavonoids in the samples were determined by high performance liquid chromatography（HPLC），and the changes of flavonoids in Yinyanghuo（Epimedii Folium）before and after processing were explored by HPLC fingerprint and multivariate statistical analysis.The osteogenic inhibition model of MC3T3-E1 cells induced by dexamethasone（DEX）was established.The anti-osteoporosis effect of Epimedium and its processed products were evaluated by measuring the expressions of alkaline phosphatase（ALP），osteoprotegerin（OPG）and osteocalcin（OCN）in cells，and the possible reasons for the different anti-osteoporosis effects of Yinyanghuo（Epimedii Folium）and its processed products were further explored.
The HPLC fingerprints of Yinyanghuo（Epimedii Folium）and its processed products were established，and the similarity of the fingerprints was evaluated，and 16 common peaks were identified.The results of cluster analysis classified epimedin A，epimedin B and epimedin C into one group，and icariin and baohuoside I into one group.The polar coordinate bubble diagram of each flavonoid content showed that the contents of primary flavonoid glycosides（epimedin A，epimedin B and epimedin C）decreased overall after processing，and the contents of secondary flavonoid glycosides（icariin and baohuoside I）increased overall after processing.The data of anti-osteoporosis activity showed that different processed products of Yinyanghuo（Epimedii Folium）had certain osteogenic proliferation effect，and the salt-processed Yinyanghuo（Epimedii Folium）had the most obvious effect on the enhancement of osteoblast activity inhibited by DEX.
Fingerprint similarity analysis and cluster analysis were used to analyze the differences in the contents of five flavonoids，so as to preliminarily explore the changes of flavonoids before and after processing. Combined with the effect of its extract on the proliferation and osteogenic ability of MC3T3-E1 cells，it was found that the content of icariin increased after salt processing of Yinyanghuo（Epimedii Folium），and the proliferation of osteogenic inhibition of MC3T3-E1 cells induced by DEX was significantly enhanced，suggesting that icariin may be the material basis for different anti-osteoporosis clinical effects of different processed Yinyanghuo（Epimedii Folium）products，laying a foundation for further exploration of the processing principle of Yinyanghuo（Epimedii Folium）.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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