Based on network pharmacology and molecular docking techniques to predict the material basis and toxicity mechanism of Chuanlianzi（Toosendan Fructus）hepatotoxicity and to validate the reducing effects after processing in combination with in vivo and in vitro experiments.

The toxic components and targets of Chuanlianzi（Toosendan Fructus）were collected using the systematic pharmacology analysis platform of traditional Chinese medicine（TCMSP）and the Swiss Target Prediction database，respectively. Liver injury related targets were obtained through GeneCards and OMIM databases. After the common targets were screened by Venny diagram，PPI analysis was carried out by STRING database，and enrichment analysis on GO function and KEGG pathway were conducted to predict their possible toxicity mechanisms. Molecular docking validation and visualization of key components of Chuanlianzi（Toosendan Fructus）hepatotoxicity with key protein binding sites and binding effects were performed using AutoDockTools and PyMOL software. In vivo experiments were conducted to compare the magnitude of acute hepatotoxicity of different processed products of Chuanlianzi（Toosendan Fructus）in mice by detecting the viability of alanine aminotransferase（ALT）and aspartate aminotransferase（AST）in serum and observing the pathologic morphology of liver tissues with HE staining method and to determine the levels of superoxide dismutase（SOD），malondialdehyde（MDA），glutathione（GSH），and glutathione peroxidase（GSH-P_X）and analyzed the reasons for the differences in the hepatotoxicity of different processed products of Chuanlianzi（Toosendan Fructus）in mice from the perspective of oxidative damage.

Network pharmacology showed that Chuanlianzi（Toosendan Fructus）hepatotoxicity components were melianone，Toosendanin and Nimbolin A，and its core targets for liver injury were TP53，IL-6 and CASP3. GO enrichment was analyzed to the biological processes，such as positive regulation of transcription and gene expression，negative regulation of the apoptotic process，and inflammatory response，cellular components，such as extracellular region，outer mitochondrial membrane，mitochondria，protein binding，protein homodimerization activity，enzyme binding and other molecular functions. KEGG pathway analysis screened TNF，PI3K-Akt，IL-17，HIF-1，apoptosis and other key signaling pathways of Chuanlianzi（Toosendan Fructus）-induced hepatotoxicity. The molecular docking results showed that the main toxic components of Chuanlianzi（Toosendan Fructus）could bind well to the core targets. In vivo experimental results showed that compared with the raw Chuanlianzi（Toosendan Fructus），the vinegar-processing，stir-frying to brown and salt-processing products all significantly reduced the activities of ALT and AST in the serum of mice（P<0.05），and the wine-processing product showed a significant increase in both ALT and AST. HE staining showed that the vinegar-processing product，stir-frying to brown and salt-processing products reduced the infiltration of inflammatory cells in the liver tissues and apoptosis of hepatocytes of mice，which suggested that Chuanlianzi（Toosendan Fructus）had the effect of alleviating liver injury after processing. This suggested that Chuanlianzi（Toosendan Fructus）had the effect of reducing liver injury，but the hepatotoxicity of the wine-processing product was greater than that of the raw product. This result suggested that the mechanism of liver toxicity induced by raw Chuanlianzi（Toosendan Fructus）in mice was related to the disruption of their antioxidant defense system，reduced ability to scavenge free radicals and subsequent excessive accumulation of free radicals in the body，which induced cell membrane lipid peroxidation.

The vinegar-processing，stir-frying to brown and salt-processing products may reduce hepatotoxicity by altering the effects of Chuanlianzi（Toosendan Fructus）on hepatotoxic targets such as TP53，IL-6 and CASP3，reducing inflammatory responses and inhibition of cell membrane lipid peroxidation，but the wine-processing product is more toxic and should be avoided in clinical practice.

To establish the fingerprint of Tenghuangtan（Dalbergia hancei Benth）by high performance liquid chromatography（HPLC）and to study the spectral-effect relationship of its antioxidant activity.

HPLC fingerprints of 10 batches of Tenghuangtan（Dalbergia hancei Benth）from different origins were established，and the antioxidant activity of Tenghuangtan（Dalbergia hancei Benth）was investigated by the 1，1-diphenyl-2-trinitrophenylhydrazine（DPPH）method and 2，2-biazobis（3-ethylbenzothiazole-6-sulfonic acid）diammonium salt（ABTS）method，and its antioxidant activity was studied by the grey relational analysis（GRA）method and partial least squares regression analysis（PLSR）method was used for its spectral-effect relationship，and then its antioxidant substance base was screened out.

The HPLC fingerprint patterns of 10 batches of Tenghuangtan（Dalbergia hancei Benth）from different origins were established，and 15 peaks were identified to characterize Tenghuangtan（Dalbergia hancei Benth）. Ten batches of Tenghuangtan（Dalbergia hancei Benth）from different origins were found to possess antioxidant activities in different degrees in a dose-dependent manner.

The HPLC fingerprints of 10 batches of Tenghuangtan（Dalbergia hancei Benth）from different places were successfully established，which provided the basis for in-depth investigation of the antioxidant efficacy of Tenghuangtan（Dalbergia hancei Benth）and its quality standard.

This study aimed to construct a deep learning model for the treatment of recurrent respiratory tract infections by using pre-training transfer learning methods.

Firstly，a large number of medical case data from Professor LI Suqing’s tutor was used as the source domain knowledge data. Based on the Sequence to Sequence（seq2seq）Algorithm of Long Short-Term Memory Network（LSTM），It combined the two attention mechanisms of symptom and drug attention to pre-train the model. Then，Professor LI Suqing＇s typical medical cases were used as target domain knowledge data for transfer learning training.

The Turing Test results showed that the error rate of participating traditional Chinese medicine doctors exceeded 30%，with the lowest error rate among those who had long-term follow-ups with Professor LI Suqing. Further qualitative and quantitative evaluation showed that the average score of the model using transfer learning method was 6.14，which was very close to the score of Professor LI Suqing＇s original prescription model of 6.50，while the score of the simple LSTM model was lower.

Pre-training transfer learning method performed well in simulating the prescription patterns of famous traditional Chinese medicine doctors，providing an effective solution for the artificial intelligence-assisted decision-making system of traditional Chinese medicine，and demonstrating the great potential of deep learning in the field of traditional Chinese medicine.

To treat the rat model of chronic eczema by Sanzi Granules（三子颗粒）and explore its mechanism of external treatment for chronic eczema.

Forty male SD rats were randomly divided into normal group，model group，Sanzi Granules group and Qingpeng Ointment（青鹏软膏）group，with 10 rats in each group. The rat models of chronic eczema were prepared by sensitization with dinitrochlorophenylacetone solution. The model group was given wet compress with normal saline，the Sanzi Granules group was given wet compress with Sanzi Granules lotion，and the Qingpeng Ointment group was externally applied with Qingpeng Ointment. Skin lesion score was used to evaluate the curative effect. HE staining method was used to observe the morphological changes of the skin tissue. The expressions of interferon gamma（IFN-γ）and interleukin-4（IL-4）in skin lesions were detected by immunohistochemical technique. The protein levels of interleukin-17（IL-17），forkhead transcription factor 3（Foxp3），phosphorylated-p38 mitogen activated protein kinase（p-p38MAPK），p38 mitogen activated protein kinase（p38MAPK），phosphorylated-nuclear factor-κB p65（p-NF-κB p65）and nuclear factor-κB p65（NF-κB p65）in skin lesions were detected by Western blot.

The model group rats showed hyperkeratosis and dyskeratosis，with significant thickening of both the granular and spinous layers，and coarser collagen fibers. In the model group，the number of mast cells was significantly increased，the protein expressions of IFN-γ and Foxp3 were decreased，and the protein expressions of IL-4，IL-17，p-p38MAPK/p38MAPK and p-NF-κB p65/NF-κB p65 were increased. Compared with those of the model group，the skin lesion scores of the Sanzi Granules group was significantly reduced on the 6th，8th and 10th day. The alleviated pathological changes and the protein expressions of IFN-γ and Foxp3 significantly increased（P<0.01），the protein expressions of IL-4，p-p38MAPK/p38MAPK and p-NF-κB p65/NF-κB p65 were significantly reduced（P<0.05，P<0.01），while the expression of IL-17 decreased，but the difference was not statistically significant（P>0.05）.

Sanzi Granules can inhibit hyperplasia of mast cells，up-regulate the protein levels of IFN-γ and Foxp3，and reduce protein levels of IL-4，IL-17，p-p38MAPK/p38MAPK and p-NF-κB p65/NF-κB p65，and play a therapeutic role in chronic eczema. These results preliminarily reveal the mechanism of Sanzi Granules treating chronic eczema.

To observe the effect of Modified Zuogui Pill（左归丸加减）combined with moxibustion in the treatment of kidney Yin deficiency type of diminished ovarian reserve，and to explore whether moxibustion combined with medicine can improve the therapeutic effect of kidney Yin deficiency type of diminished ovarian reserve.

A total of 60 patients meeting the diagnostic criteria for kidney Yin deficiency type of diminished ovarian reserve in the gynecological outpatient department of Huaibei Hospital of Traditional Chinese Medicine of Anhui Province from January 2022 to December 2022 were collected as research subjects，and were randomly divided into treatment group and control group，with 30 patients in each group. The control group received moxibustion treatment，and the treatment group received Modified Zuogui Pill and moxibustion treatment. Both groups were treated for 3 consecutive menstrual cycles. The levels of serum estradiol（E2），follicle-stimulating hormone（FSH）and luteinizing hormone（LH）of the two groups were measured before and after treatment. Basal sinus follicle count and endometrial thickness were monitored before and after treatment，and TCM syndrome scores of the two groups were recorded before and after treatment.

The total effective rate of the treatment group and the control group was 93.33% and 73.33%，respectively（P<0.05）. After treatment，the level of E2，endometrial thickness and sinus follicle count in the treatment group were higher than those in the control group，and the levels of FSH，LH and the scores of small abdominal pain during menstruation，delayed menstruation and dark color or purple were lower than those in the control group（P<0.05）.

Zuogui Pill combined with moxibustion has significant curative effect in the treatment of kidney Yin deficiency type of diminished ovarian reserve，symptoms and hormone levels are significantly improved，which is suitable for popularization and application.

To observe the effect of Danggui Buxue Decoction（当归补血汤）on bone marrow hematopoietic function in mice with aplastic anemia（AA），and explore the balance of Th17/Treg cells and the role of related inflammatory factors.

A mouse model of AA was prepared by combining radiation irradiation with allogeneic mouse lymphocyte infusion. After successful modeling，the participants were randomly divided into a model control group，Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）1∶1 group，Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）3∶1 group and Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）5∶1 group. A normal control group was set up，and each group was given the corresponding drugs by gavage once a day for 28 days. The general condition，body weight，spleen index and basic hematological parameters of peripheral blood in mice were observed，and the pathological changes of bone marrow tissue were observed through HE staining. ELSIA method was used to detect the expression levels of inflammatory factor proteins such as interleukin-17A（IL-17A），interleukin-6（IL-6），transforming growth factor-β1（TGF-β1）and interleukin-10（IL-10）. Flow cytometry was used to detect the effects of Danggui Buxue Decoction on the proportion of Th17 and Treg cells and the balance of Th17/Treg cells in mouse bone marrow.

After the intervention of Danggui Buxue Decoction，in the Danggui Buxue Decoction treatment groups，the general symptoms of the mice improved，the food and water intake increased，the body weight and spleen index significantly increased，the peripheral blood red blood cell，white blood cell，platelet count and hemoglobin concentration significantly elevated（P<0.05），the bone marrow hematopoietic function improved，and the hematopoietic tissue proliferation increased（P<0.05）. The protein expressions of IL-17A and IL-6 were significantly down-regulated，while the protein expressions of TGF-β1 and IL-10 were significantly up-regulated（P<0.05 or P<0.01）. The proportion of Th17 cells in bone marrow decreased significantly，while the proportion of Treg cells increased significantly. The Th17/Treg cell ratio decreased significantly（P<0.05 or P<0.01），with the Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）5∶1 group showing the best effect.

Danggui Buxue Decoction can improve the bone marrow hematopoietic function in mice with AA，and its mechanism of action may be related to regulating the ratios of Th17 and Treg cells，intervening the balance of Th17/Treg cells，and regulating the inflammatory factor levels such as IL-17A，IL-6，TGF-β1 and IL-10.

By observing the effect of Taoren（Persicae Semen）on cough variant asthma（CVA）model rats，it discussed the effect and mechanism of Taoren（Persicae Semen）in relieving cough and asthma.

Smoke combined with ovalbumin（OVA）-aluminium hydroxide sensitisation and nebulisation of OVA sensitising solution method was used to establish a CVA rat model to evaluate the efficacy of Taoren（Persicae Semen）in relieving cough and calming asthma by observing the general morphology of the rats，determining the incubation period of cough，the number of coughs and lung function and observing the pathological changes in the lung tissues of the rats with the HE staining method. Masson staining was used to measure collagen deposition in rat lung tissues. HE staining was used to measure airway morphological parameters of bronchial tubes. Enzymelinked immunosorbent assay（ELISA）method was used to measure the levels of interferon-γ（IFN-γ），interleukin-4（IL-4），interleukin-5（IL-5），interleukin-13（IL-13），nerve growth factor（NGF）in rat bronchoalveolar lavage fluid（BALF），and immunohistochemistry method forα-smooth muscle actin（α-SMA）expression，to explore the mechanism of action of Taoren（Persicae Semen）in relieving cough and asthma.

After 2 weeks of continuous administration，compared with those of the CVA model group，the general morphology of rats in each dose group of Taoren（Persicae Semen）could be improved to different degrees. The body weight of rats was significantly increased（P<0.05）. The incubation period of cough was significantly prolonged（P<0.01）and the number of cough was significantly decreased（P<0.01）. The lung function of rats could be improved in different degrees in each administration group. The infiltrated area of inflammatory cells in lung tissue was reduced to different degrees，and the alveolar cavity became wider. The exfoliation of trachea epithelial cells，inflammatory cell infiltration and vascular congestion were improved to a certain extent. In addition，IFN-γ/IL-4 levels were significantly increased in each administration group（P<0.05，P<0.01）. The levels of IL-5 and IL-13 were decreased in different degrees，the collagen area around trachea was decreased，the ratios of Wat/Pbm and Wam/Pbm were decreased，andα-SMA expression was down-regulated.

Taoren（Persicae Semen）can improve the symptoms of cough and asthma in CVA rats，and have relieving cough and asthma effects. The mechanism may be as follows.①It corrects the imbalance of Th1/Th2 and reduce the infiltration of Eos，thereby reducing airway inflammation.②It decreases IL-13 concentration and reduces Wat/Pbm and Wam/Pbm to inhibit AHR.③It down-regulatesα-SMA expression and inhibits airway smooth muscle thickening and collagen deposition to slow down airway remodeling.

To investigate the protective effect of acupuncture on the reproductive function of mice by observing the effect of the“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture method on key factors in the death receptor pathway of testicular tissue in asthenospermia mice.

Thirty C57BL/6 male mice were randomly divided into a blank group，a model group and an acupuncture group（n＝10）. The blank group was given an equal volume of 0.9% sodium chloride solution by gavage，while the model group and acupuncture group were given intraperitoneal injection of cyclophosphamide（50 mg·kg^-1·d^-1）for 5 days. After the completion of modeling，the acupuncture group were given continuous acupuncture treatment with“Zhibian（BL54）-to-Shuidao（ST28）”for 2 weeks，once a day，20 minutes for each time. The other two groups were only fixed，and the samples were taken the next day after treatment. In daily observations，the general condition of mice was assessed，and the changes in body weight and testicular and epididymal indices were recorded. The sperm quality of the mice was evaluated. Enzyme-Linked Immunosorbent Assay（ELISA）was used to measure the levels of testosterone（T），follicle-stimulating hormone（FSH）and luteinizing hormone（LH）in the serum. Hematoxylin-Eosin（HE）staining was employed to examine the pathological morphology of testicular tissue. Terminal deoxynucleotidyl transferase dUTP nick end labeling（TUNEL）staining was used to observe cell apoptosis in testicular tissue. Fluorescent quantitative polymerase chain reaction（PCR）and Western blot were employed to assess changes in the mRNA and protein expressions of Fas cell surface death receptor（Fas），Fas ligand（FasL），tumor necrosis factor alpha（TNF-α），TNF receptor 1（TNFR1），tumor necrosis factor-related apoptosis-inducing ligand（TRAIL），death receptor 5（DR5），Caspase-8 and Caspase-3 in testicular tissue. Immunofluorescence staining was conducted to detect the positive expressions of cleaved Caspase-3 and PARPin testicular tissue.

Compared with the control group，the model group exhibited a decrease in body weight of male mice（P<0.01），a decrease in testicular and epididymal mass and coefficients（P<0.01），a decrease in total sperm count，sperm motility and sperm viability（P<0.01），a decrease in serum levels of testosterone（T），follicle-stimulating hormone（FSH）and luteinizing hormone（LH）（P<0.01），deterioration in the pathological morphology of testicular and epididymal tissues，an increase in the number of positively stained apoptotic cells（P<0.01），an increase in the mRNA and protein expression levels of FasL，Fas，TNF-α，TNFR1，FADD，Caspase-8 and Caspase-3（P<0.01，P<0.05）. There was no significant difference in the expression levels of TRAIL，DR5 mRNA or protein（P>0.05），and an increase in the expressions of cleaved Caspase-3 and PARP（P<0.01）. Compared to the model group，the acupuncture group showed no significant change in body weight after treatment（P>0.05）. There was an increase in testicular and epididymal mass and coefficients（P<0.01）. The levels of total sperm count，sperm motility and sperm viability increased（P<0.01）. The serum levels of testosterone（T），follicle-stimulating hormone（FSH）and luteinizing hormone（LH）increased（P<0.01）. There was an improvement in the pathological morphology of testicular and epididymal tissues，a decrease in the number of positively stained apoptotic cells（P<0.01），a decrease in the mRNA and protein expression levels of FasL，Fas，TNF-α，TNFR1，FADD，Caspase-8 and Caspase-3（P<0.01，P<0.05）. There was no significant difference in the expression levels of TRAIL，DR5 mRNA and protein（P>0.05）or decrease in the expression of cleaved Caspase-3 and PARP（P<0.01）.

The“Zhibian（BL54）-to-Shuidao（ST28）”technique can improve spermatogenic cell apoptosis in oligospermia mice and enhance reproductive function，and its mechanism may be associated with the regulation of key factors in the testicular tissue death receptor apoptosis pathway.

To observe the clinical efficacy of Zhuyang Tongbian Decoction（助阳通便汤）on patients with functional constipation（spleen and kidney Yang deficiency）and the effects of serum substance P（SP）and vasoactive intestinal peptide（VIP）as well as its effects on the histopathology of the colon and the expression of VIPin the constipation model mice.

（1）Sixty-six patients who met the diagnosis of functional constipation and whose TCM syndrome was spleen and kidney Yang deficiency were grouped by randomized numerical table method，including the treatment group（33 cases）and the control group（33 cases）. The treatment group was given Zhuyang Tongbian Decoction（130 mL）orally twice a day，and the control group was given Lactulose（15 mL）orally twice a day. Both groups were treated for 2 weeks. The constipation symptom scores，fecal character score，traditional Chinese medicine symptom scores and contents of serum SPand VIPbefore and after the use of drugs in the two groups were compared.（2）Eighteen BALB/c mice were divided into KB group（blank control group），MX group（model group）and ZY group（Zhuyang Tongbian Decoction group）according to the randomized numerical table method，6 mice in each group were modeled by gavage with 80 mg/kg of diphenoxylate tablets suspension，and ZY group was given Zhuyang Tongbian Decoction（0.2 mL/10 g）for two weeks，and then samples were taken out to observe the histopathology of colon and VIP immunohistochemical expressions of each group. Colonic histopathology and VIPimmunohistochemical expressions were observed in each group.

（1）The scores of constipation symptoms and fecal character of patients in both groups after treatment were significantly higher than those before treatment（P<0.05）. There was a significant difference in TCM symptom scores in the treatment group（P<0.05）. The serum SP content of patients in the treatment group was higher than that of the control group（P<0.05），and the serum VIPcontent was lower than that of the control group（P<0.05）. The effective rate was 90.0% in the treatment group and 73.3% in the control group（P<0.05）.（2）Compared with the mice in the model group（MX group），the distribution of VIP-positively expressing cells was relatively denser and the number of positive cells was higher in the colonic tissues of the mice in the ZY group（P<0.05）.

The clinical symptoms and traditional Chinese medicine symptoms of patients with functional constipation（spleen and kidney Yang deficiency）were improved by Zhuyang Tongbian Decoction，and it also had an intervention effect on the contents of serum SPand VIPof the patients. The modulation of VIPcontent in the colon tissue of mice with constipation by Zhuyang Tongbian Decoction is one of its mechanisms of action in the treatment of functional constipation.

To observe the effect of acupuncture treatment on coexpression of neuronal nitric oxide synthase（nNOS）and postsynaptic dense-95（PSD-95）in the hippocampus of depression model rats.

Fifty SPF grade rats were randomly divided into six groups：normal group，model group（intraperitoneal injection of 0.2 mg·kg^-1·d^-1 of reserpine），acupuncture group，ZL006 group，acupuncture combined with ANA-12 group，8-OH-DPAT group. There were 9 rats in the normal group and model group，and 8 rats in each of the remaining groups. The changes in body weight and anal temperature were observed. Open field test，sucrose preference test and forced swimming test were applied to observe depression-like behaviors in rats. The morphological changes of hippocampal neurons were observe by HE staining. Immunofluorescence double staining was used to observe the positive expression of nNOSand PSD-95，as well as tyrosine kinase receptor B（TrkB）and PSD-95 in neurons of the hippocampal CA1 and CA3 regions.

There were coexpressions of nNOSand PSD-95 as well as TrkB and PSD-95 in neurons of the hippocampal CA1 and CA3 regions. Compared with those in the model group，depression-like behaviors were significantly improved in the acupuncture group and ZL006 group（P<0.05，P<0.01，P<0.001）. The coexpression levels of nNOSand PSD-95 in the CA3 region were significantly down-regulated（P<0.05，P<0.01），and the coexpression levels of TrkB and PSD-95 in the CA3 region were significantly up-regulated（P<0.05）. The reduction and damage of hippocampal neurons were significantly improved. The expression levels of nNOSin the hippocampal CA1 region were significantly down regulated（P<0.01）and the expression levels of TrkB in the CA1 region were significantly up-regulated（P<0.01）in the acupuncture group. The expression level of nNOSin the CA1 region of the 8-OH-DPAT group was significantly down-regulated（P<0.01）. Compared with those in the acupuncture group，the coexpression levels of nNOSand PSD-95 in the CA3 region of the ANA-12 group were significantly up-regulated（P<0.05），and the above differences were statistically significant.

Acupuncture regulates Shaoyang pivot，which may participate in the antidepressant effect by up-regulating the coexpression levels of nNOSand PSD-95 in hippocampal CA3 neurons，and this mechanism of action may be related to TrkB receptors in the CA3 region.

To explore the potential molecular mechanisms underlying the occurrence and development of liver cancer based on bioinformaticsand to screen and validate the mechanisms and targets of Biejiajian Pill（鳖甲煎丸，BJJP）on liver cancer.

Single-cell data of hepatocellular carcinoma（HCC）was downloaded from the GEO database，processedand subjected to communication analysis，enrichment and metabolic analysis，and reanalysis of cellular subpopulations. Subsequently，the components and action targets of BJJP were collected，and in conjunction with transcriptomic results，the intersection targets between BJJP and HCC were identified and analyzed. Finally，protein and small molecule information was obtained from the PDB and PubChem databases to simulate drug-target molecular docking. A computational dynamics simulation environment was constructed to assess the interactions.

Transcriptomic analysis revealed that intercellular communication was more active in the peritumoral group of HCC. The cells were predominantly enriched in pathways related to cell cycle checkpoints and immune responses. The hepatocyte metabolism was active，while immune cell metabolism was suppressed. The natural killer（NK）cells gradually evolved from possessing normal cytotoxic capabilities to lower capabilities，and hepatocytes gradually transformed from normal to cancerous cells. Network pharmacology results showed that BJJP potentially regulated 124 targets in HCC，mostly enriched in pathways such as immune response，inflammatory response，glycolysisand interleukin-17. Molecular docking was performed with key proteins and potential drug action targets，and the top three with the strongest binding affinity were subjected to dynamic simulation，which showed that these three receptor-ligand complexes were relatively stable.

HCCcells may promote malignant proliferation and immune escape by disrupting cell cycle and immune functions. BJJP may achieve antitumor effects by regulating pathways such as anti-inflammatory，immune responseand cell division. The key drug-target relationships were validated with good results in molecular docking and molecular dynamics，demonstrating their favorable binding affinity.

In order to study the different chemical constituents of Mandiya Hongdoushan（Taxus media Rehd）before and after processing.

The chemical components were identified by ultra-high performance liquid chromatography（UHPLC-Q-Orbitrap HRMS）in Mandiya Hongdoushan（Taxus media Rehd）with quadrupole electrostatic field orbital well high resolution mass spectrometry，and the chemical components were identified according to the precise molecular weight and fragment ion information，reference products，literature，online database，etc. The ionic peak area ratio of each component before and after processing was used as the change index. Principal component analysis（PCA）and orthogonal partial least square discriminant analysis（OPLS-DA）models of different processed Mandiya Hongdoushan（Taxus media Rehd）were established by SIMCA software. The values of PCA，OPLS-DA and variable importance in the projection（VIP）were obtained to screen the different components of Mandiya Hongdoushan（Taxus media Rehd）before and after processing.

A total of 74 components were identified in the experiment，including 74 components of Mandiya Hongdoushan（Taxus media Rehd）raw products，70 components of wine processing products，70 components of wine soaking products，71 components of wine washing products，71 wine steaming products，71 components of vinegar processing products，71 components of vinegar soaking products，71 components of vinegar steaming products，72 components of honey-prepared products and 70 components of ginger stir-fried products. Among them，18 compounds were identified by comparison with control substances. PCA showed that the chemical composition of Mandiya Hongdoushan（Taxus media Rehd）was significantly different in different processing methods. OPLS-DA screened 38 chemical components with VIPvalue>1 as the main difference components of different processing methods.

The results show that the method is rapid and accurate，and can be used to determine the chemical composition difference of Mandiya Hongdoushan（Taxus media Rehd）before and after processing，which can provide important reference for clinical efficacy study.

Huangqi（Astragali Radix）has a good effect in anti peritoneal fibrosis（PF）. Analyzing and summarizing the therapeutic effect and mechanism of Huangqi（Astragali Radix）on PF provides ideas for future research on PF.

The relevant literature on the prevention and treatment of PF by Huangqi（Astragali Radix）was retrieved from databases such as PubMed，CNKI and Wanfang. The pharmacological basis and mechanism of action of Huangqi（Astragali Radix）treating PF were analyzed and summarized.

According to traditional Chinese medicine theory and pharmacological analysis of Huangqi（Astragali Radix）against PF，Huangqi（Astragali Radix）can intervene in the process of PF by regulating inflammation，reducing oxidative stress，inhibiting epithelial to mesenchymal transition（EMT）of peritoneal mesothelial cells（PMC），improving metabolic disorders，regulating PMC apoptosis and regulating pro fibrotic factors.

The active ingredients contained in Huangqi（Astragali Radix）have high anti fibrosis activity，which can protect the integrity of peritoneal structure and function in multiple directions and targets.

To explore whether ethyl acetate extract of Liujunzi Decoction（EAELD）can regulate the energy metabolism of TE-1 of esophageal cancer cells via the microRNA-1（miR-1）/hypoxia inducible factor-1α（HIF-1α）axis.

Stable transfection blocking miR-1 expression cell lines（miR-1 inhibitor，IN）and blank controls（miR-1 NC，NC）have been established in the preliminary experiments. The half maximal inhibitory concentration（IC₅₀）and 30% inhibitory concentration（IC₃₀）of EAELD on NC were obtained using MTT assay，and were used as the Liujunzi Decoction high and low concentrations to compare their effects on IN and NC，respectively. The effect of EAELD on miR-1 and HIF-1αmRNA in TE-1 cells was measured by quantitative real-time PCR（RT-qPCR）. Dual-luciferase gene reporter assay was used to validate the targeting relationship of miR-1 and HIF-1α. Then the experiments were divided into NC，Liujunzi Decoction low concentration，Liujunzi Decoction high concentration，IN＋Liujunzi Decoction low concentration and IN＋Liujunzi Decoction high concentration groups. Seahorse energy metabolism analysis system detected the mitochondrial pressure and glycolysis rate，and the protein expression level was detected by Western Blot.

MTT results showed that EAELD could significantly inhibit the proliferation of NCin a concentration gradient dependent manner. The low and high concentration groups were 20 μg/mL（IC₃₀）and 40 μg/mL（IC₅₀），respectively. The RT-qPCR results showed that the EAELD high concentration group significantly promoted the gene expression of miR-1（P<0.05）and HIF-1αshowed a downward trend，without statistically significant difference. Dual-luciferase gene reporter assay showed that the fluorescence intensity of wild type（WT）＋mimics group was significantly lower than that of WT＋negative control（mimics NC）group（P<0.05），and there was no significant difference in the fluorescence intensity of mutant（MUT）＋mimics group compared with that of MUT＋mimics NCgroup. When high and low concentrations of EAELD were applied to IN，the MTT results showed that compared with that of NCgroup，the inhibitory effect of EAELD on IN group was significantly reduced（P<0.05）. Seahorse energy metabolism results showed that compared with those of NC group，the ratio of mitochondrial oxygen consumption to glycolysis（P<0.01，P<0.05），glycolytic potential（P<0.01），basal respiration value，proton leakage oxygen consumption（P<0.01，P<0.05），proton leakage oxygen consumption（P<0.01，P<0.05），mitochondrial maximum oxygen consumption capacity and spare respiratory capacity（P<0.01）were inhibited in the high and low concentration groups of Liujunzi Decoction. The high concentration group could inhibit basal glycolysis，compensate glycolysis，non-mitochondrial oxygen consumption and ATPsynthesis oxygen consumption（P<0.01）. Compared with those of NC＋Liujunzi Decoction low concentration group，basal glycolysis，mitochondrial to basal glycolysis ratio，glycolytic potential（P<0.05）and oxygen consumption for ATPsynthesis（P<0.01）were significantly increased in IN＋Liujunzi Decoction low concentration group. Compared with those of NC＋Liujunzi Decoction high concentration group，basal glycolysis，compensatory glycolysis（P<0.05），basal respiratory capacity，proton leakage oxygen consumption，maximum mitochondrial oxygen consumption and non-mitochondrial oxygen consumption（P<0.01）were significantly increased in IN＋Liujunzi Decoction high concentration group. WB results showed that compared with NCgroup，EAELD high and low concentration groups significantly decreased the protein relative expression levels of HK2（P<0.01），GLUT1（P<0.05，P<0.01）and MCT1（P<0.01，P<0.05）. The relative protein expressions of PKM2（P<0.05），MCT2 and MCT4（P<0.01）in the high concentration of EAELD group were significantly decreased. Compared with those of NC＋Liujunzi Decoction low concentration group，the expressions of HK2，GLUT1，PKM2（P<0.01），p-PKM2，MCT1 and MCT2（P<0.05）in IN＋Liujunzi Decoction low concentration group were significantly increased. Compared with those of NC＋Liujunzi Decoction high concentration group，the expressions of HK2，GLUT1，PKM2（P<0.01），p-PKM2，MCT1 and MCT2（P<0.05）in IN＋Liujunzi Decoction low concentration group were significantly increased. IN＋Liujunzi Decoction high concentration group could significantly promote the expressions of HK2，GLUT1，PKM2 and MCT4（P<0.05）.

EAELD can inhibit the energy metabolism of esophageal cancer cell line TE-1 by down-regulating the expressions of GLUT1，PKM2，p-PKM2，HK2，MCT1，MCT2 and MCT4，and the mechanism is partly related to miR-1/HIF-1αaxis.

To investigate the regulatory effect of Modified Chaihu Shugan Decoction（加味柴胡疏肝汤）on epileptic mice through the protein kinases 1（RIP1）/protein kinases 3（RIP3）/mixed lineage kinase domain-like protein（MLKL）pathway.

Sixty KM mice were randomly divided into normal group，model group，low dose group of Modified Chaihu Shugan Decoction［19.52 g/（kg· d）］，medium dose group of Modified Chaihu Shugan Decoction［39.04 g/（kg·d）］，high dose group of Modified Chaihu Shugan Decoction［78.08 g/（kg·d）］and carbamazepine group［30 mg/（kg·d）］. Epilepsy models were prepared using pilocarpine hydrochloride. After continuous gavage for two weeks，the general condition and electroencephalogram（EEG）of the mice were observed. Hematoxylin-eosin（HE）staining was used to observe pathological and morphological changes. TdT-mediated dUTP Nick-End Labeling（TUNEL）method was used to detect the apoptosis rate of neural cells. Quantitative real time polymerase chain reaction（qRT-PCR）and Western Blot（WB）measured the mRNA and protein expression levels of RIP1，RIP3，MLKL，tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β）and interleukin-6（IL-6）in the hippocampus of the mice.

Compared with the normal group，the body weight of the model group mice was decreased significantly；EEG can clearly observe continuous epileptic waves；HE staining showed that many neurons were pyknotic，with reduced cell volume and irregular morphology. The boundaries between the nucleus and cytoplasm were unclear，and the staining deepened；The positive rate of apoptotic cells significantly increased；The relative expression levels of RIP1，RIP3，MLKL，TNFα，IL-1βand IL-6 were significantly increased. After drug treatment，the weight of mice in each group increased slightly compared to that of the model group. The frequency and intensity of epileptic discharges in EEG waves were significantly reduced. The positive rates of apoptotic cells in the medium dose and high dose groups of Modified Chaihu Shugan Decoction and carbamazepine group were significantly lower than those in the model group，with statistical significance. The relative expression levels of mRNA and protein of RIP1，RIP3，MLKL，TNF-α，IL-1βand IL-6 in the high dose group of Modified Chaihu Shugan Decoction and the carbamazepine group were significantly lower than those of the model group，indicating statistical significance.

Modified Chaihu Shugan Decoction may exert its anti epileptic effect through the RIP1/RIP3/MLKL signaling pathway.

To explore the potential mechanism of Guizhi Fuling Pill（桂枝茯苓丸，GFW）in the treatment of adenomyosis（AM）by combining gut microbiota and metabolomics technology.

Seven-week-old ICR mice were randomized assigned to the control，model and GFW groups. The AM model was developed by the allogeneic pituitary transplantation method. The three groups were sacrificed after 8 weeks of intragastric administration. Histopathological changes of the mice uterus were evaluated，and the frequency and amplitude of uterine contraction in vitro were detected.16SrRNA gene sequencing was utilized to compare the changes in the Alpha and Beta diversity of the intestinal flora before and after GFW intervention，and to screen the differential gut microbiota. The changes of gut metabolites after GFW treatment were explored using liquid chromatography-mass spectrometry（LC-MS）. Correlation analysis was performed on the differential microbiota and metabolites to find out the possible mechanism of GFW regulating AM，followed by experimental validation.

HE staining confirmed that the AM model was successfully formed. Compared with those of the model group，the amplitude of uterine contraction in vitro was significantly decreased after GFW treatment（P<0.05），and the contraction frequency showed a downward trend. The Alpha diversity of gut microbiota showed that there was no significant difference in Observed species and Ace index among the three groups. The distribution of gut microbiota varied significantly between the control and model groups，according to the Beta diversity results. GFW treatment was able to modulate the gut flora composition of AM mice to varying degrees. Further analysis indicated that the abundance of Bacteroidetes and Bacteroides in the intestine of AM mice was significantly down-regulated（P<0.01，P<0.01），whereas the relative abundance of Firmicutes and Tenericutes was significantly up-regulated（P<0.05，P<0.05）. After GFW intervention，the abundance of Tenericutes，Mycoplasma and Enterorhabdus was significantly decreased（P<0.05，P<0.05，P<0.01），while Parabacteroides was significantly increased（P<0.05）. The results of metabolomics screening proved that the AM mice had 22 intestinal differential metabolites related to oxidative stress，immunity and uterine smooth muscle contraction，such as prostaglandin D₃，glutamic acid，tryptophan，hydroxyeicosatetraenoic acid and stercobilin. Correlation analysis displayed parts of the gut microbiota were significantly correlated with the metabolites. Subsequent experimental verified the levels of reactive oxygen species（ROS），tumor necrosis factor-α（TNF-α）and interleukin-17（IL-17）were significantly higher in AM mice. The GFW treatment demonstrated a significant reduction in expression levels of ROSand TNF-α.

GFW can improve uterine smooth muscle contraction，oxidative stress and immune response in AM mice，which may be related to the regulation of gut microbiota and intestinal metabolites. Its specific regulatory mechanism needs to be confirmed by further studies.

To explore the clinical observation of Fuyuan Huoxue Decoction（复元活血汤）combined with acupuncture in the treatment of coronary heart disease patients with Qi deficiency and blood stasis combined with chronic heart failure.

A total of 106 patients with coronary heart disease of Qi deficiency and blood stasis type combined with chronic heart failure admitted to the hospital from January 1，2020 to December 31，2021 were collected as research subjects. Using a random number table method，the patients were divided into an experimental group and a control group，with 53 cases in each group. The control group received Aspirin Enteric Coated Tablets（100 mg，once a day），Isosorbide Mononitrate Tablets（20 mg，3 times a day），Spironolactone Tablets（20 mg，once a day），Metoprolol Tartrate Tablets（25 mg，3 times a day）and Captopril Tablets（25 mg，3 times a day）. On this basis，the experimental group applied Fuyuan Huoxue Decoction combined with acupuncture treatment. The TCM syndrome scores，clinical efficacy，heart rate，blood pressure and incidence of adverse reactions were compared between two groups. The research data in this article was analyzed by using SPSS 25.0 statistical software.

After treatment，compared with the control group，the experimental group had a higher clinical total effective rate（P<0.05）. After treatment，the scores of various traditional Chinese medicine syndromes in both groups decreased compared to those before treatment（P<0.05）. Compared with the control group，the experimental group had lower scores of various traditional Chinese medicine syndromes（P<0.05）. After treatment，the values of heart rate（HR），systolic blood pressure（SBP）and diastolic blood pressure（DBP）of the two groups were significantly reduced compared to those before treatment（P<0.05）. Compared with those of the control group，the levels of HR，SBPand DBPin the experimental group were lower（P<0.05）. There was no significant difference in the incidence of adverse reactions between the two groups of patients（P>0.05）.

The combination of Fuyuan Huoxue Decoction and acupuncture treatment can significantly improve the clinical symptoms of patients with Qi deficiency and blood stasis type of coronary heart disease combined with chronic heart failure，with high safety. Its treatment mechanism may be closely related to inflammation inhibition and regulation of hemodynamics.

To observe the effect of Duhuo Jisheng Decoction（独活寄生汤）on Toll-like receptor 4（TLR4）/nuclear transcription factorκB（NF-κB）signaling pathway in elderly patients with knee osteoarthritis.

A total of 118 elderly patients with knee osteoarthritis were divided into observation group and control group（59 cases in each）according to random number table method. The control group was treated with Glucosamine Hydrochloride Tablets and the observation group was treated with Duhuo Jisheng Decoction on the basis of the control group，4 weeks for 1 course of treatment，a total of 2 courses of treatment. The clinical efficacy of the two groups was compared，and the TCM syndrome scores，Western Ontario and McMaster University Osteoarthritis Index（WOMAC）scale score and visual analogue scale（VAS）score before and after treatment were compared between the two groups. Serum levels of interleukin-6（IL-6），matrix metalloproteinase-1（MMP-1），osteocalcin（BGP），carboxy-terminal peptideβdegradation product of type I procollagen（β-CTX）and carboxy-terminal peptide（PICP）of type I procollagen，and the protein expressions of TLR4 and NF-κB were observed before and after treatment in the two groups.

After treatment，the total effective rate of the observation group was 96.61%，higher than that（83.05%）of control group，and the difference was statistically significant（P<0.05）. After treatment，the scores of TCM syndrome in both groups were lower than those before treatment，and the score of the observation group was lower than that of the control group（P<0.05）. After treatment，the WOMACscores and VASscores of both groups were lower than those before treatment，and the scores of the observation group were lower than those of the control group（P<0.05）. After treatment，the levels of IL-6，MMP-1，TLR4 and NF-κB protein in two groups were lower than those before treatment，and the levels of the observation group were lower than those of the control group（P<0.05）. After treatment，the levels of serum BGP and PICP of the two groups were higher than those before treatment，and the levels of the observation group were higher than those of the control group，and the serumβ-CTX level of the two groups was lower than that before treatment，and the level of the observation group was lower than that of the control group（P<0.05）.

The application of Duhuo Jisheng Decoction in the elderly patients with knee osteoarthritis is beneficial to improve the clinical efficacy，reduce the pain，fatigue and other symptoms，and enhance the joint function. The mechanism of action may be related to improving bone metabolism indexes，inhibiting inflammatory factors through TLR4/NF-κB signaling pathway and alleviating cartilage damage.

In order to explore the effect of Modified Jiegeng Yuanshen Decoction（桔梗元参汤加减）in the treatment of allergic rhinitis（AR）mediated by advanced glycation end product（AGE）-receptor for advanced glycation end product（RAGE）signaling pathway.

A total of 260 patients with AR treated in the hospital from January 2022 to June 2023 were selected and divided into a control group（130 cases）and an observation group（130 cases）by random number table method. The control group was given loratadine，and the observation group was treated with Modified Jiegeng Yuanshen Decoction of the hospital. The total effective rate，clinical symptoms，expression levels of AGE and RAGE proteins，inflammatory indicators［nuclear factor kappa-B（NF-κB），interleukin-6（IL-6），reactive oxygen species（ROS）］and adverse reactions were compared between the two groups.

Compared with that of the control group，the total effective rate of the observation group was significantly higher（P<0.05），and the clinical symptom scores were significantly decreased（P<0.05），the expression levels of AGEand RAGEproteins and the levels of NF-κB，IL-6 and ROSwere significantly decreased（P<0.05）. Mild adverse reactions such as dizziness，fatigue and gastrointestinal discomfort occurred in both groups. The incidence of adverse reactions in the observation group was slightly lower than that in the control group，but there was no significant difference（P>0.05）.

Modified Jiegeng Yuanshen Decoction can reduce the degree of inflammatory response in patients with AR by inhibiting AGE-RAGE/NF-κB/IL-6 and AGE-RAGE/ROSsignaling pathways，thereby improving the treatment efficiency and relieving clinical symptoms.

The aim was to investigate the effects of Jianpi Fuzheng Runzao Decoction（健脾扶正润燥汤）on the survival and toxic side effects of chemotherapy patients with advanced breast cancer.

A hundred and twenty cases of breast cancer patients in the hospital were included and randomly divided into observation group and control group according to the random number table method，with 60 cases in each group. The control group was given conventional treatment with western medicines，and the observation group was given Jianpi Fuzheng Runzao Decoction on the basis of the control group. The clinical benefit rate，immune function，median survival，median progression-free survival and the occurrence of toxic side effects were observed before and after treatment in both groups.

After treatment，the clinical benefit rate of the observation group was 73.33%（44/60），which was significantly higher than that（53.33%，32/60）of the control group（P<0.05）. After 2 cycles of treatment，the blood routine indexes of the two groups were lower than those before treatment（P<0.05）. After 4 cycles of treatment，the blood routine indexes of the two groups increased，and the improvement of the observation group was significantly better than that of the control group（P<0.05）. After treatment，the levels of immunoglobulin（Ig）G，IgA and IgM of both groups were significantly higher than those before treatment（P<0.05），and the improvement of the indicators of the observation group was significantly better than that of the control group（P<0.05）. The toxic side effects of both groups were classⅠ～Ⅱ，and the incidence rate of toxic side effects was 23.33%（14/60）in the observation group，which was significantly lower than that（45.00%，27/60）in the control group（P<0.05）. After treatment，the median survival（OS）time of the observation group and the control group was 14.2 months and 11.6 months，respectively，and the time of the observation group was better than that of the control group（P<0.05）. The median progression-free survival（PFS）time of the observation group and the control group was7 months and 5.3 months，respectively，and the time of the observation group was better than that of the control group（P<0.05）.

Jianpi Fuzheng Runzao Decoction has significant efficacy in patients with advanced breast cancer chemotherapy，which can effectively improve the survival and immune function，and reduce the incidence of toxic side effects in patients.

To explore the therapeutic effect of Xuefu Zhuyu Decoction（血府逐瘀汤）combined with warm acupuncture and moxibustion on the pain of lumbar disc herniation and its influence on pain mediators and inflammatory factors.

Eighty-six patients with lumbar disc herniation pain admitted to the Second Affiliated Hospital of Heilongjiang University o Chinese Medicine from February 2022 to August 2023 were selected and divided into a study group and a control group using a random number table method，with 43 cases in each group. The control group was treated with warm acupuncture and moxibustion，while the study group was treated with Xuefu Zhuyu Decoction on the basis of the control group. After 14 days of treatment，the traditional Chinese medicine symptoms，efficacy，degree of pain，serum pain mediators and inflammatory factors，and daily activity ability of the two groups of patients were compared，and adverse reactions during the treatment period were recorded.

After treatment，the main symptom scores and secondary symptom scores decreased in both groups（P<0.05）. The main symptom scores and secondary symptom scores in the study group were lower than those in the control group after treatment（P<0.05）. The total effective rate in the study group was higher than that in the control group（P<0.05）. Both groups showed a decrease in scores of back pain，radiating pain，referred pain and neuropathic pain after treatment（P<0.05）. The study group had lower scores of back pain，radiating pain，referred pain and neuropathic pain than the control group after treatment（P<0.05）. The levels of serotonin（5-HT），prostaglandin E2（PGE2）and tumor necrosis factor-alpha（TNF-α）decreased in both groups after treatment（P<0.05）. The study group had lower levels of 5-HT，PGE2 and TNF-αthan the control group after treatment（P<0.05）. The activities of daily living（ADL）scores increased（P<0.05），and the Oswestry Disability Index（ODI）scores decreased in both groups after treatment（P<0.05）. The study group had higher ADL scores than the control group（P<0.05），and ODI scores were lower than those of the control group（P<0.05）. There was no statistically significant difference in the incidence of adverse reactions between the two groups（P>0.05）.

Xuefu Zhuyu Decoction combined with warm acupuncture and moxibustion is helpful to relieve the pain of patients with lumbar disc herniation，inhibit the expressions of pain and inflammatory factors，and improve the ability of daily activities of patients.

To observe the clinical effect of five-element acupuncture and moxibustion reusing the source acupoints combined with Ningshen Wendan Decoction（宁神温胆汤）on schizophrenic auditory hallucinations.

A total of 82 patients with phlegm-fire disturbance type of schizophrenia who were treated in the hospital from June 2021 to January 2023 were selected and divided into conventional group（41 cases）and traditional Chinese medicine（TCM）group（41 cases）by random number table method. The conventional group was given risperidone oral treatment，and the TCM group was given five-element acupuncture reusing the source acupoints combined with Ningshen Wendan Decoction on this basis. TCM syndrome scores，Positive and Negative Symptom Scale（PANSS）and Auditory Hallucinations Rating Scale（AHRS）scores，brain neurotransmitter levels，clinical efficacy and adverse reactions were observed in two groups.

After treatment，TCM syndrome scores，PANSS and AHRSscores and dopamine（DA）level in TCM group were lower than those in the conventional group（P<0.05），and 5-hydroxytryptamine（5-HT）level was higher than that in the conventional group（P<0.05）. The total effective rate of the TCM group was higher than that of the conventional group（P<0.05）. There was no significant difference in the total incidence of adverse reactions between the two groups（P>0.05）.

The five-element acupuncture reusing the source acupoints method combined with Ningshen Wendan Decoction can effectively improve the clinical symptoms such as auditory hallucinations，the levels of brain neurotransmitters，the treatment efficiency and safety.

To investigate the effect of Guling Cream（骨灵膏）on bone regeneration and chondrocyte apoptosis in rabbit knee cartilage defect model.

Thirty male rabbits（New Zealand）were randomly divided into model group，blank control group and Guling Cream low，medium and high dose groups，with 6 rabbits in each group. The model of knee cartilage defect was established by modified joint scoring method. The blank control group did not drill，and the Guling Cream low，medium and high dose groups were given Guling Cream with dose of 1.16 mL/kg（3.70 g/kg），3.47 mL/kg（11.10 g/kg），10.41 mL/kg（33.31 g/kg）by gavage，respectively. The the model group blank and control group were given 3.47 mL/kg normal saline，once every 3 days for 12 weeks. The Wakitani score was used for histological score. Masson staining was used for pathological evaluation，and terminal transferase-mediated dUTP nick end labeling（TUNEL）method was used to detect synovial and chondrocyte apoptosis. The levels of superoxide dismutase（SOD），nitric oxide（NO），lipid peroxide（LPO）in synovial fluid and pyridine phenol（PYD）in urine were detected by enzyme-linked immunosorbent assay.

At the 12th week after administration，the wound area in the model group was uneven，and the cartilage defect area in each dose group was filled in. The surface of the low dose group was rough，while the medium dose group showed less smoothness. The surface of the high dose group was relatively smooth，and the joint cartilage structure was good. Compared with those in the model group，the arrangement of chondrocytes in each dose group was more regular，and chondrocytes secreted more collagen fibers. The histological scores and apoptosis rates of synovial and chondrocytes in the low，medium and high dose groups of were higher than those in the control group and lower than those in the model group. As the dose increased，the histological scores and apoptosis rates of synovial and chondrocytes showed a decreasing trend（P<0.05）. The levels of joint fluid NO and LPO and urine PYD in the low，medium and high dose groups were higher than those in the control group，but lower than those in the model group. As the dose increased，the expression levels showed a descending trend，and the difference was statistically obvious（P<0.05）. The SOD levels in the joint fluid of the low，medium and high dose groups were lower than those of the control group and higher than those of the model group. As the dose increased，the expression level showed an growing trend，and the difference was statistically obvious（P<0.05）.

Guling Cream can improve the cartilage injury and accelerate the repair of cartilage tissue in rabbits with knee cartilage defect possibly by regulating the expressions of oxidative stress factors to inhibit chondrocyte apoptosis and promote cartilage tissue regeneration.