Full
Outline
To observe the effect of Danggui Buxue Decoction（当归补血汤）on bone marrow hematopoietic function in mice with aplastic anemia（AA），and explore the balance of Th17/Treg cells and the role of related inflammatory factors.
A mouse model of AA was prepared by combining radiation irradiation with allogeneic mouse lymphocyte infusion. After successful modeling，the participants were randomly divided into a model control group，Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）1∶1 group，Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）3∶1 group and Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）5∶1 group. A normal control group was set up，and each group was given the corresponding drugs by gavage once a day for 28 days. The general condition，body weight，spleen index and basic hematological parameters of peripheral blood in mice were observed，and the pathological changes of bone marrow tissue were observed through HE staining. ELSIA method was used to detect the expression levels of inflammatory factor proteins such as interleukin-17A（IL-17A），interleukin-6（IL-6），transforming growth factor-β1（TGF-β1）and interleukin-10（IL-10）. Flow cytometry was used to detect the effects of Danggui Buxue Decoction on the proportion of Th17 and Treg cells and the balance of Th17/Treg cells in mouse bone marrow.
After the intervention of Danggui Buxue Decoction，in the Danggui Buxue Decoction treatment groups，the general symptoms of the mice improved，the food and water intake increased，the body weight and spleen index significantly increased，the peripheral blood red blood cell，white blood cell，platelet count and hemoglobin concentration significantly elevated（P<0.05），the bone marrow hematopoietic function improved，and the hematopoietic tissue proliferation increased（P<0.05）. The protein expressions of IL-17A and IL-6 were significantly down-regulated，while the protein expressions of TGF-β1 and IL-10 were significantly up-regulated（P<0.05 or P<0.01）. The proportion of Th17 cells in bone marrow decreased significantly，while the proportion of Treg cells increased significantly. The Th17/Treg cell ratio decreased significantly（P<0.05 or P<0.01），with the Huangqi（Astragali Radix）-Danggui（Angelicae Sinensis Radix）5∶1 group showing the best effect.
Danggui Buxue Decoction can improve the bone marrow hematopoietic function in mice with AA，and its mechanism of action may be related to regulating the ratios of Th17 and Treg cells，intervening the balance of Th17/Treg cells，and regulating the inflammatory factor levels such as IL-17A，IL-6，TGF-β1 and IL-10.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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