To establish the fingerprint of Tenghuangtan(Dalbergia hancei Benth)by high performance liquid chromatography(HPLC)and to study the spectral-effect relationship of its antioxidant activity.
HPLC fingerprints of 10 batches of Tenghuangtan(Dalbergia hancei Benth)from different origins were established,and the antioxidant activity of Tenghuangtan(Dalbergia hancei Benth)was investigated by the 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)method and 2,2-biazobis(3-ethylbenzothiazole-6-sulfonic acid)diammonium salt(ABTS)method,and its antioxidant activity was studied by the grey relational analysis(GRA)method and partial least squares regression analysis(PLSR)method was used for its spectral-effect relationship,and then its antioxidant substance base was screened out.
The HPLC fingerprint patterns of 10 batches of Tenghuangtan(Dalbergia hancei Benth)from different origins were established,and 15 peaks were identified to characterize Tenghuangtan(Dalbergia hancei Benth). Ten batches of Tenghuangtan(Dalbergia hancei Benth)from different origins were found to possess antioxidant activities in different degrees in a dose-dependent manner.
The HPLC fingerprints of 10 batches of Tenghuangtan(Dalbergia hancei Benth)from different places were successfully established,which provided the basis for in-depth investigation of the antioxidant efficacy of Tenghuangtan(Dalbergia hancei Benth)and its quality standard.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |