Full
Outline
In order to study the different chemical constituents of Mandiya Hongdoushan（Taxus media Rehd）before and after processing.
The chemical components were identified by ultra-high performance liquid chromatography（UHPLC-Q-Orbitrap HRMS）in Mandiya Hongdoushan（Taxus media Rehd）with quadrupole electrostatic field orbital well high resolution mass spectrometry，and the chemical components were identified according to the precise molecular weight and fragment ion information，reference products，literature，online database，etc. The ionic peak area ratio of each component before and after processing was used as the change index. Principal component analysis（PCA）and orthogonal partial least square discriminant analysis（OPLS-DA）models of different processed Mandiya Hongdoushan（Taxus media Rehd）were established by SIMCA software. The values of PCA，OPLS-DA and variable importance in the projection（VIP）were obtained to screen the different components of Mandiya Hongdoushan（Taxus media Rehd）before and after processing.
A total of 74 components were identified in the experiment，including 74 components of Mandiya Hongdoushan（Taxus media Rehd）raw products，70 components of wine processing products，70 components of wine soaking products，71 components of wine washing products，71 wine steaming products，71 components of vinegar processing products，71 components of vinegar soaking products，71 components of vinegar steaming products，72 components of honey-prepared products and 70 components of ginger stir-fried products. Among them，18 compounds were identified by comparison with control substances. PCA showed that the chemical composition of Mandiya Hongdoushan（Taxus media Rehd）was significantly different in different processing methods. OPLS-DA screened 38 chemical components with VIPvalue>1 as the main difference components of different processing methods.
The results show that the method is rapid and accurate，and can be used to determine the chemical composition difference of Mandiya Hongdoushan（Taxus media Rehd）before and after processing，which can provide important reference for clinical efficacy study.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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