Full
Outline
To observe the effect of acupuncture treatment on coexpression of neuronal nitric oxide synthase（nNOS）and postsynaptic dense-95（PSD-95）in the hippocampus of depression model rats.
Fifty SPF grade rats were randomly divided into six groups：normal group，model group（intraperitoneal injection of 0.2 mg·kg-1·d-1 of reserpine），acupuncture group，ZL006 group，acupuncture combined with ANA-12 group，8-OH-DPAT group. There were 9 rats in the normal group and model group，and 8 rats in each of the remaining groups. The changes in body weight and anal temperature were observed. Open field test，sucrose preference test and forced swimming test were applied to observe depression-like behaviors in rats. The morphological changes of hippocampal neurons were observe by HE staining. Immunofluorescence double staining was used to observe the positive expression of nNOSand PSD-95，as well as tyrosine kinase receptor B（TrkB）and PSD-95 in neurons of the hippocampal CA1 and CA3 regions.
There were coexpressions of nNOSand PSD-95 as well as TrkB and PSD-95 in neurons of the hippocampal CA1 and CA3 regions. Compared with those in the model group，depression-like behaviors were significantly improved in the acupuncture group and ZL006 group（P<0.05，P<0.01，P<0.001）. The coexpression levels of nNOSand PSD-95 in the CA3 region were significantly down-regulated（P<0.05，P<0.01），and the coexpression levels of TrkB and PSD-95 in the CA3 region were significantly up-regulated（P<0.05）. The reduction and damage of hippocampal neurons were significantly improved. The expression levels of nNOSin the hippocampal CA1 region were significantly down regulated（P<0.01）and the expression levels of TrkB in the CA1 region were significantly up-regulated（P<0.01）in the acupuncture group. The expression level of nNOSin the CA1 region of the 8-OH-DPAT group was significantly down-regulated（P<0.01）. Compared with those in the acupuncture group，the coexpression levels of nNOSand PSD-95 in the CA3 region of the ANA-12 group were significantly up-regulated（P<0.05），and the above differences were statistically significant.
Acupuncture regulates Shaoyang pivot，which may participate in the antidepressant effect by up-regulating the coexpression levels of nNOSand PSD-95 in hippocampal CA3 neurons，and this mechanism of action may be related to TrkB receptors in the CA3 region.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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