To observe the effects of volatile oil of Gaoliangjiang（Alpinia officinarum Hance），Dagaoliangjiang（Alpinia galanga Willd）and Hongdoukou（Alpinia galanga Willd）on body temperature and sympathetic nerve-adrenal axis in rats with stomach cold syndrome and to explore the material basis of warm properties of three Chinese drugs of Alpinia.

A total of 150 SPF grade SD male rats were selected，and 10 rats were randomly selected as the blank group.The other rats were simulated the stomach cold syndrome by oral administration of Zimu decoction.After modeling，they were randomly divided into model group，positive group，volatile oil of Gaoliangjiang（Alpinia officinarum Hance）high and low dose groups，volatile oil of Dagaoliangjiang（Alpinia galanga Willd）high and low dose groups，volatile oil of Hongdoukou（Alpinia galanga Willd）high and low dose groups，Gaoliangjiang（Alpinia officinarum Hance）high and low dose groups，Dagaoliangjiang（Alpinia galanga Willd）high and low dose groups，Hongdoukou（Alpinia galanga Willd）high and low dose groups.With gingerol as the positive control，the anal temperature was determined by electronic thermometer，the body temperature of upper abdomen and mid-abdomen was determined by infrared thermograph，and the contents of norepinephrine（NE），adrenocorticotropic hormone（ACTH），corticotropin releasing hormone（CRH），dopamine（DA），dopamine-β-hydroxylase（D-β-H），serotonin（5-HT），acetylcholinesterase（AchE）and 17 hydroxycorticosteroids（17-OHCS）were determined by ELISA.

Compared with those of the blank group，the contents of NE，ACTH，CRH，DA，D-β-H，AchE and 17-OHCSin serum of rats with stomach cold syndrome were significantly decreased.The content of 5-HT was significantly increased.Compared with that of the model group，the content of ACTH in all other groups，except that in the volatile oil of Dagaoliangjiang（Alpinia officinarum Hance）low dose group and Hongdoukou（Alpinia officinarum Hance）volatile oil groups，was significantly increased.The 17-OHCScontent was significantly increased in all groups except that in the low dose group of Gaoliangjiang（Alpinia officinarum Hance）.The CRH content of rats was significantly increased except that in low dose groups of Gaoliangjiang（Alpinia officinarum Hance）and Hongdoukou（Alpinia galanga Willd）.The D-β-H was significantly increased except that in low dose groups of Dagaoliangjiang（Alpinia galanga Willd）and Hongdoukou（Alpinia galanga Willd）.The DA content of rats was significantly increased and the 5-HTwas significantly decreased except those in the Hongdoukou（Alpinia galanga Willd）group.The AchE was significantly increased except that in the low dose group of Gaoliangjiang（Alpinia officinarum Hance）.The NE in each administration group was significantly increased.

The essential oil of three Chinese drugs of Alpinia can regulate the function of sympathetic nerve and adrenal axis in rats with stomach cold syndrome.

To evaluate the knowledge and clinical capabilities of large language models in the field of traditional Chinese medicine.

Using literature research and experimental research methods，9 different categories of large language models were selected，a data set of knowledge ability and clinical ability was constructed，and traditional Chinese medicine professional ability prompts were designed.The automatic evaluation method and expert scoring method were used to evaluate the large language models，and the variance was used.Analysis and multiple comparison methods were used to comparatively analyze the medical professional ability level of large language models.

In the evaluation of knowledge ability，Lingyi Wanwu（78.93），Zhipu Qingyan（77.91）and Tongyi Qianwen（77.22）performed best.There were significant differences in the knowledge ability scores of different models，different subjects and different types of models（P<0.05）.In the clinical capability evaluation，the responses generated by each model had high legibility（81.00），but low accuracy（74.86）.Among them，the average score of Wenxin Yiyan was the highest at 85.40，and all five-dimension scores reached excellent levels（≥80）.The difference in clinical ability scores of different types of models was significant.

The general large language model has advantages in generalization，legibility and security.The medical large language model performs well overall in the field of traditional Chinese medicine specialization and traditional Chinese medicine consultation.The application of large language models in the field of traditional Chinese medicine has broad development prospects in the future.

To investigate the effect and mechanism of Cynomorium songaricum flavonoids（CSF）on cognitive dysfunction in senescence-accelerated mice prone 8（SAMP8）.

Fifty 7-month-old male SAMP8 were randomly divided into control group，low，intermediate and high dose groups of CSF［25，50，100 mg/（kg·d）］，and donepezil group.The low，intermediate and high dose groups were given a corresponding dose of CSF to SAMP8 mice by continuous gavage for 20 days，while the donepezil group was given donepezil［0.71 mg/（kg·d）］to SAMP8 mice by gavage for 20 days.The spatial learning and memory performance was evaluate by the Morris water maze experiment after administration，and the morphology and count of hippocampal pyramidal cells was observed by Nissl staining.The apoptosis-related protein expression levels and the levels of nuclear factor E2 related factor 2（Nrf2），Kelch epichlorohydrin associated protein 1（Keap1）and quinone oxidoreductase-1（NQO1）in the hippocampus of each group were detected by Western blotting.The serum levels of malondialdehyde（MDA）content as well as glutathione peroxidase（GSH-Px）and superoxide dismutase（SOD）activities were measured by ELISA.

CSF significantly reduced the escape latency and path length，while increased the number of crossing platforms and the percentage of target quadrant time（P<0.05）.CSF improved the morphology and quantity of pyramidal cells in hippocampal CA1 region.Western blot results showed that compared with the control group，the protein expression levels of Nrf2，Keap1 and NQO1 in the hippocampus of the intermediate dose group，high dose group and donepezil group all significantly increased（P<0.05），the ratio of Bcl-2/Bax protein expression in the hippocampus of all administration groups of CSF and donepezil group increased substantially（P<0.05），and the expressions of Caspase-3 protein in the hippocampus of the intermediate dose group，high dose group and donepezil group all decreased significantly（P<0.05）.Compared with the control group，CSF significantly increased the serum activities of SOD and GSH-Px in SAMP8 mice（P<0.05），while remarkedly decreased the content of MDA（P<0.05）.

CSF can significantly improve spatial learning and memory impairment in Alzheimer＇s disease，and its mechanism may be related to inhibiting oxidative stress and cell apoptosis.

To observe the effects of acupuncture combined with neuromuscular electrical stimulation on shoulder joint function and nerve function after arthroscopic rotator cuff repair.

A hundred and fifty patients who visited the Affiliated Hospital of Henan University due to shoulder joint injury from December 2021 to December 2023 were selected as the research subjects.They were randomly divided into a control group and an observation group using a random number table method.The control group received neuromuscular electrical stimulation treatment，while the observation group received acupuncture combined with neuromuscular electrical stimulation.The excellent rate，adverse reactions，pain level，shoulder joint range of motion，shoulder joint function and nerve function were compared between the two groups at different time points before and after intervention.

The total effective rate of the observation group（96.00%，72/75）was significantly better than that of the control group（86.67%，65/75）（P<0.05）.At week 2，4 and 6 of intervention，the visual analogue scale（VAS）score of the shoulder joint in the observation group was significantly reduced（P<0.05，P<0.01）.After the intervention was completed，the active flexion angle and lateral rotation angle of the shoulder joint in the observation group were significantly increased compared to those in the control group（P<0.01），and the National Institutes of Health Stroke Scale（NIHSS）score of the observation group was significantly down regulated，while the American Shoulder and Elbow Surgeons’Form（ASES）score was significantly up regulated（P<0.01）.After 3 months of intervention，the 36-Item Short-Form（SF-36）score of the observation group patients was significantly up-regulated compared to that of the control group（P<0.01）.The incidence of adverse reactions in the observation group（12.00%，9/75）showed no significant difference compared to that in the control group（10.67%，8/75）（P>0.05）.

Acupuncture combined with neuromuscular electrical stimulation can significantly alleviate the postoperative pain in ARCR，expand the range of motion of the shoulder joint，promote the joint function recovery，improve the neurological function，reduce the shoulder muscle atrophy and enhance the quality of life.

To investigate the effect of Modified Baihe Gujin Decoction（加味百合固金汤）on CD⁺₄ T cell subsets and prognosis of patients with rifampicin-sensitive pulmonary tuberculosis of lung Yin deficiency syndrome.

A total of 94 patients with rifampicin-sensitive pulmonary tuberculosis of lung Yin deficiency syndrome admitted to the Tuberculosis Department of Zhengzhou Sixth People＇s Hospital from September 2020 to September 2023 were selected as the study objects，and were divided into study group and control group by simple random number table with 47 cases in each group.The control group received 2HRZE/4HRE anti-tuberculosis regimen.On the basis of the control group，the study group was treated with Modified Baihe Gujin Decoction for 2 months.TCM symptoms integral，immune function indexes［serum immunoglobulin G（IgG），immunoglobulin A（IgA），immunoglobulin M（IgM）］and CD⁺₄ T cell subsets were compared between the two groups before and after treatment.The lesion absorption，negative conversion rate and the occurrence of adverse reactions were compared during treatment.

After treatment，the scores of hot flash，dry throat，phlegm and night sweat in both groups were significantly decreased，and the score of the study group was significantly lower than that of the control group（P<0.05）.After treatment，the levels of IgG，IgA，IgM and CD⁺₄ T cell subsets were significantly increased in both groups，and those in the study group were significantly higher than those in the control group（P<0.05）.The lesion absorption rate and negative conversion rate of the study group were higher than those of the control group（P<0.05）.There was no significant difference in the incidence of adverse reactions between two groups（P>0.05）.

Modified Baihe Gujin Decoction is effective in the treatment of rifampicinsensitive pulmonary tuberculosis of lung Yin deficiency syndrome，which can significantly improve the immune function and prognosis of patients，and has high safety.

To analyze the therapeutic effect of Tianma Gouteng Yin（天麻钩藤饮）on hypertension and the mechanism of regulating mitochondrial protein pathway.

Ninety-two patients with hypertension admitted to the hospital from January 2023 to December 2023 were included as study objects，and were divided into two groups by random number table method.Forty-six patients in the control group were treated with nifedipine sustained-release tablet，and the other forty-six patients in the observation group were treated with Tianma Gouteng Yin on this basis.The curative effect，blood pressure level，symptom score，oxidative stress index and mitochondrial fusion protein level were compared between the two groups.

The total curative effect rate of the observation group was 95.65%（44/46），which was much higher than that of the control group（76.09%，35/46），and the difference was significant（P<0.05）.After treatment，the systolic blood pressure，diastolic blood pressure，vertigo，headache，lumbo-knee acid and five upset heat in the observation group were lower than those in the control group（P<0.05）.After treatment，the levels of glutathione peroxidase（GSH-Px）and superoxide dismutase（SOD）in the observation group were higher than those in the control group，the levels of malondialdehyde（MDA）and reactive oxygen species（ROS）were lower than those in the control group（P<0.05）.After treatment，the levels of recombinant mitofusin 1（MFN1），recombinant mitofusin 2（MFN2）and optic nerve atrophy protein 1（OPA1）in the observation group were higher than those in the control group（P<0.05）.

The application of Tianma Gouteng Yin in the treatment of hypertensive patients can effectively improve the symptoms of patients，reduce the level of blood pressure，delay the effect of vascular aging by enhancing the expression of mitochondrial fusion protein，reduce the oxidative stress reaction，improve the clinical efficacy，and has high application value.

To analyze the effect of Liuwei Dihuang Pill（六味地黄丸）on muscle atrophy in patients with diabetic peripheral neuropathy.

A total of 80 patients with diabetic peripheral neuropathy treated in the hospital from November 2022 to March 2024 were included in the study.They were divided into two groups by random number table method.Forty patients in the control group were treated with conventional western medicine，and the other 40 patients in the observation group were treated with Liuwei Dihuang Pill on the basis of control group.The treatment effect，blood glucose change and nerve conduction velocity change of Toronto Clinical Scoring system（TCSS）were compared between the two groups.

The total effective rate of the observation group was 95.0%（38/40），which was much higher than that of the control group（72.5%，29/40），and the difference was significant（P<0.05）.After treatment，TCSSscore and blood glucose index of the observation group were lower than those of the control group（P<0.05）.After treatment，motor nerve conduction velocity（MNCV）and sensory nerve conduction velocity（SNCV）of common peroneal nerve and median nerve in the observation group were higher than those in the control group（P<0.05）.After treatment，the average latency，dispersion and abnormality rate of F waves in the median nerve，ulnar nerve，common peroneal nerve and tibial nerve in the observation group were lower than those in the control group（P<0.05）.

The application of Liuwei Dihuang Pill in the treatment of diabetic peripheral neuropathy can effectively improve the numbness，pain and other related symptoms，reduce the severity of neuropathy，increase the nerve conduction speed and relieve the muscle atrophy，and plays a positive role in the prognosis of patients.

To analyze the clinical effect of Modified Xiaoke Decoction（消渴方加减）treating patients with type 2 diabetic nephropathy（Qi deficiency and blood stasis type）.

A total of 220 patients with type 2 diabetic nephropathy of Qi deficiency and blood stasis type admitted to the Department of Endocrinology and Nephrology of Ziyang Hospital of Traditional Chinese Medicine from June 2020 to June 2024 were included as the research objects.They were divided into two groups by the random number table method.A hundred and ten patients in the control group were treated with conventional western medicine treatment，and the other 110 patients in the observation group were treated with Modified Xiaoke Decoction on this basis.The treatment effects，TCM symptom scores，blood glucose indexes，isletβcell functions，renal function indexes and changes in oxidative stress indexes of the two groups were compared.

The total effective rate of the observation group was 97.28%（107/110），which was much higher than that of the control group（82.73%，91/110），and the difference between the groups was significant（P<0.05）.After treatment，the levels of fasting blood glucose（FPG），2-hour postprandial blood glucose（2 h PBG），glycated hemoglobin（HbA1c）and homeostasis model assessment of insulin resistance（HOMA-IR）in the observation group were lower than those in the control group，and homeostasis model assessment-β（HOMA-β）was higher than that in the control group（P<0.05）.After treatment，the levels of 24-hour urinary protein and urinaryβ₂-microglobulin（β₂ MG）in the observation group were lower than those in the control group（P<0.05）.After treatment，the levels of superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）in the observation group were higher than those in the control group，and the level of malondialdehyde（MDA）was lower than that in the control group（P<0.05）.

The application of Modified Xiaoke Decoction in patients with type 2 diabetic nephropathy of Qi deficiency and blood stasis type can effectively reduce the blood glucose level，improve insulin resistance and isletβcell function，inhibit the oxidative stress reaction，protect renal function，reduce damage，and has a positive effect on the improvement of the prognosis of patients.

To explore the therapeutic effect of Juanbi Decoction（蠲痹汤）assisted with eramode and methotrexate in the treatment of rheumatoid arthritis.

A total of 82 patients with rheumatoid arthritis admitted from May 2021 to May 2023 were evenly divided into western medicine group and combined decoction group by random number table method，with 41 cases in each group.The patients in the western medicine group were treated with eramode and methotrexate，while those in the combined decoction group were treated with eramode and methotrexate，supplemented by Juanbi Decoction.In the course of treatment，2 cases fell off in the western medicine group and 1 case fell off in the combined decoction group.The data of patients in each group was compared，including the clinical efficacy，the TCM symptom scores（morning stiffness，joint dampness and swelling pain，weak mouth，sore waist and knee，heavy limbs，etc.）before and after treatment，the numbers of joint redness，swelling and pain，the time of morning stiffness，the changes in erythrocyte sedimentation rate（ESR）and rheumatoid factor（RF）levels，the levels of interleukin-17（IL-17）and tumor bad factorα（TNF-α），joint disease motion（DAS28）score of patients after 1，2 and 3 months of treatment and treatment adverse reactions.

The total effective rate of the combined decoction group was higher than that of the western medicine group（P<0.05）.After treatment，TCM symptom scores（morning stiffness，joint damp，cold and swollen pain，weak mouth and no thirst，waist and knee soreness，limb heaviness，etc.）of patients in both groups were decreased，the number of joint redness，swelling and pain was decreased，the time of morning stiffness was shortened，and the levels of ESR，RF，IL-17 and TNF-αwere significantly decreased.After treatment in the combined decoction group，the TCM syndrome scores（morning stiffness，joint dampness and swelling pain，weak mouth，sore waist and knee，heavy limb，etc.），the number of joint redness，swelling and pain，the morning stiffness time，the levels of ESR，RF，IL-17 and TNF-αwere significantly lower than those in the western medicine group（P<0.05）.After 1，2 and 3 months of treatment，the DAS28 score of the combined decoction group was significantly lower than that of the western medicine group（P<0.05）.The adverse reaction rate of the combined decoction group was lower than that of the western medicine group（P<0.05）.

Juanbi Decoction assisted eramod and methotrexate in the treatment of rheumatoid arthritis has a good effect，and the symptoms of patients can be significantly improved after treatment with few adverse reactions.The treatment is safe and feasible.

To investigate the effect of Banxia Xiexin Decoction（半夏泻心汤）on the function of pancreaticβcells and Gprotein-coupled receptor kinase2（GRK2）/sweet taste receptor（STR）signaling pathway in vivo and in vitro in prediabetes，so as to provide references for clinical application of Banxia Xiexin Decoction in the prevention and treatment of prediabetes.

The obese prediabetic mouse model was prepared by using 60% high-fat diet，and the mice were randomly divided into control group，model group and Banxia Xiexin Decoction group.After continuous administration of the drug for 4 weeks，the oral glucose tolerance（OGTT）and fasting insulin levels of the mice in each group were detected.The morphology of pancreatic islets was observed under paraffin sections with hematoxylin-eosin（HE）staining，the number of pancreaticβ/αcells was detected by immunofluorescence，the expressions of taste 1 receptor member 3（TAS1R3）and GRK2 were detected by immunohistochemistry method and immunofluorescence，respectively.Western blot（WB）and RT-qPCR were employed to measure the protein and mRNA expressions of TAS1R3 and GRK2 in pancreatic tissue.INS-1 pancreaticβcells were applied to be treated with Banxia Xiexin Decoction-containing serum and/or high glucose（25 mmol/L）for 24 h.Insulin secretion was detected by ELISA under low and high glucose stimulation，while the protein expressions of TAS1R3 and GRK2 were by WB.

Compared with the model group，Banxia Xiexin Decoction group exhibited a significant weight loss effect（P<0.001）.In comparison with the control group，the model group showed elevated blood glucose at each time point of OGTT，as well as the area under the glucose curve，and fasting insulin levels（P<0.01）；also the volume of islets increased，and theβ/αratio decreased significantly.The expression of TAS1R3 was down-regulated，while GRK2 expression was up-regulated.After the treatment of Banxia Xiexin Decoction，the blood glucose，the area under the blood glucose curve of OGTT and the fasting insulin level of the mice were significantly decreased（P<0.01）.The morphology and structure of islets were more regular，the volume of islets decreased，and the number of islet cells was close to that of normal islets.Banxia Xiexin Decoction restored the expression of TAS1R3 and down-regulated GRK2 expression.In vitro experiments also demonstrated that Banxia Xiexin Decoction could restore the insulin secretion disorder induced by high glucose，up-regulate the expression of TAS1R3 and down-regulate GRK2 expression.

Banxia Xiexin Decoction can effectively regulate blood glucose，protect pancreatic isletβcell function and treat prediabetes.The mechanism of action may be related to its regulation of sweet taste receptor signaling pathway ofβcells.

To explore the effects of ethanol extract from Tengligen（Radix Actinidiae Chinensis）on apoptosis and malignant biological behavior of non-small cell lung cancer.

The logarithmic growth stage non-small cell lung cancer cells A549 were selected to add different concentrations ethanol extract of Tengligen（Radix Actinidiae Chinensis）（0，10，20，40 μg/mL）.The proliferation inhibition rate of A549 cells was measured by using MTT assay.Annexin V-FITCwas used to measure of A549 cells apoptosis rate.Western Blot was used to determine the expressions of apoptotic proteins Bax and Bcl-2.The invasive ability of A549 cells was measured by using Transwell method.The scratch healing rate of A549 cells was measured by using scratch experiments.

The cell proliferation inhibition rates of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.The Bax grayscale values of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL，while the Bcl-2 grayscale values were lower than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and showed a dose-dependent effect.The cell invasion rate of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.The scratch healing rate of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.

Ethanol extract of Tengligen（Radix Actinidiae Chinensis）can inhibit cell proliferation，invasion and migration，and induce apoptosis in non-small cell lung cancer A549 cells.It was related to Bax expression up-regulation and Bcl-2 expression down-regulation.

To analyze the multilevel effects of Shenqi Decoction（参芪汤）combined with chemotherapy on immune protein factor and autophagy protein in the treatment of colorectal cancer.

A total of 92 patients with colorectal cancer admitted to the hospital from January 2021 to December 2023 were divided into two groups by random number table method.Forty-six patients in the control group were treated with chemotherapy，and the other 46 patients in the observation group were treated with Shenqi Decoction combined with chemotherapy.The difference of efficacy，tumor markers，angiogenesis factor levels，immune function and autophagy related proteins LC3 and Beclin 1 were compared between the two groups.

The objective remission rate and disease control rate in the observation group were 41.30%（19/46）and 93.48%（43/46），respectively，which were higher than 26.09%（12/46）and 76.09%（35/46）in the control group（P<0.05）.After treatment，the levels of tumor markers［cancer antigen 125，carbohydrate antigen 125（CA125）and cancer antigen 19-9（CA19-9）］，vascular endothelial growth factor（VEGF），hypoxia-inducible factor 1-alpha（HIF-1α）and transforming growth factorβ1（TGF-β1）in the observation group were lower than those in the control group（P<0.05）.After treatment，the positive rates of autophagy related proteins LC3 and Beclin 1 were lower than those of the control group，and the levels of immunoglobulin A（IgA），immunoglobulin G（IgG）and immunoglobulin M（IgM）were higher than those of the control group（P<0.05）.

Shenqi Decoction combined with chemotherapy can effectively improve the therapeutic effect of colorectal cancer patients，reduce the levels of serum tumor markers，regulate the immune ability，inhibit the growth of tumor cells and angiogenesis，reduce the expressions of autophagy related proteins LC3 and Beclin 1，and plays a positive role in improving the prognosis of patients.

To observe the effect of Cangfu Daotan Decoction（苍附导痰汤）on the insulin receptor substrate-1（IRS-1）and glucose transporter type 4（GLUT4）signaling pathways in rats with polycystic ovary syndrome-insulin resistance（PCOS-IR）.

Sixty SPF-grade female SD rats were randomly divided into blank group，model group，Cangfu Daotan Decoction high-dose group，Cangfu Daotan Decoction low-dose group and metformin group，with 12 rats in each group.Except for the blank group，the other groups were induced PCOS-IR mdoel by subcutaneous injection of dehydroepiandrosterone（DHEA）combined with a high-fat diet for 20 days，while the blank group was injected with an equal volume of physiological saline.After the model was established，the rats in each group were administered the respective drugs by gavage for 28 days：metformin（0.265 g·kg^-1）and Cangfu Daotan Decoction（10.82，21.64 g·kg^-1）.The changes in body weight were recorded，and the samples were collected after the last administration.The intraperitoneal glucose tolerance test（IPGTT）was performed using a blood glucose meter to calculate the homeostatic model assessment of insulin resistance（HOMA-IR）and the area under the glucose curve（GAUC）.Hematoxylin-eosin（HE）staining was used to observe ovarian tissue under a light microscope and to count the numbers of primary follicles，secondary follicles and mature follicles.Enzyme-linked immunosorbent assay（ELISA）was used to measure serum levels of luteinizing hormone（LH），follicle-stimulating hormone（FSH），testosterone（T），estradiol（E2）and insulin（INS）.The protein expression levels of IRS1，phosphatidylinositol 3-hydroxy kinase（PI3K），serine/threonine kinase proteins（AKT）and GLUT4 in ovarian tissue were detected by Western blot.

Compared with the blank group，the model group had significantly higher body weight and levels of IPGTT，HOMA-IR and GAUC，significantly lower serum AMH levels，significantly higher levels of LH，FSH，T，E2 and INS，significantly fewer primary follicles，significantly more mature follicles，significantly higher IRS1 protein expression in ovarian tissue，and significantly lower protein expression levels of PI3K，AKT and GLUT4（P<0.05，P<0.01）.Compared with the model group，the Cangfu Daotan Decoction high-dose group and metformin group showed significantly lower body weight and levels of IPGTT，HOMA-IR and GAUC.The Cangfu Daotan Decoction high-dose group had significantly more primary follicles and significantly fewer mature follicles，while the metformin group and Cangfu Daotan Decoction high-dose group had significantly lower serum levels of E2，LH/FSH and T，significantly higher IRS1 protein expression in ovarian tissue，and significantly lower protein expression levels of PI3K，AKT and GLUT4（P<0.05，P<0.01）.Compared with the metformin group，the Cangfu Daotan Decoction high-dose group showed significantly lower levels of IPGTT，HOMA-IR and GAUC，significantly lower levels of LH/FSH and T，and significantly higher protein expression levels of PI3K，AKT and GLUT4 in ovarian tissue（P<0.05，P<0.01）.The result in the Cangfu Daotan Decoction high-dose group was closer to that in the blank group.

Cangfu Daotan Decoction significantly improves ovarian function in PCOS-IR rats，and its mechanism may be related to the inhibition of the IRS1/PI3K/AKT/GLUT4 signaling pathway.

To analyze the effect of Fangji Huangqi Decoction（防己黄芪汤）combined with acupuncture and Tuina on simple obesity in children with spleen deficiency and dampness-blocking syndrome based on“correspondence of prescription and syndrome”.

A total of 80 children with simple obesity with spleen deficiency and dampness-blocking syndrome treated in the hospital from January 2023 to December 2024 were included as the study objects.They were divided into two groups by random number table method.Forty cases in the control group were given basic sports nutrition treatment，and the other forty cases in the observation group were combined with acupuncture and Tuina treatment based on“prescription and syndrome corresponding”.The treatment effect and obesity indexes including weight，waist circumference，body mass index，TCM syndrome score，blood lipid level and glucose metabolism related indexes were compared between the two groups.

The total effective rate of the observation group was 95.0%（38/40），which was much higher than that of the control group（72.5%，29/40），and the difference was significant（P<0.05）.After treatment，the scores of obesity，edema，body weight，fatigue，waist circumference，hip circumference，body mass index（BMI）and waist-hip ratio in the observation group were lower than those in the control group（P<0.05）.After treatment，the levels of total cholesterol（TC），triglycerides（TG）and low-density lipoprotein cholesterol（LDL-C）in the observation group were lower than those in the control group，and high-density lipoprotein cholesterol（HDL-C）was higher than that in the control group（P<0.05）.After treatment，the levels of fasting plasma glucose（FPG），fasting insulin（FINS）and homeostatic model assessment of insulin resistance（HOMA-IR）in the observation group were lower than those in the control group（P<0.05）.

For simple obesity children with spleen deficiency and dampness-blocking syndrome，Fengji Huangqi Decoction combined with acupuncture and Tuina based on“correspondence of prescription and syndrome”can effectively reduce body fat parameters such as body mass，waist circumference and BMI，regulate blood lipid levels，improve glucose and lipid metabolism，and help to reduce health risks brought by obesity，improve the quality of life and overall health level.

Autophagy is involved in the pathological changes of traumatic optic neuropathy（TON），and the regulation of autophagy mediated by the adenosine monophosphate activated kinase（AMPK）-mammalian target of rapamycin（mTOR）-ULK pathway is a potential therapeutic approach.AstragalosideⅣ（AS-Ⅳ）can regulate autophagy and plays a therapeutic role in a variety of diseases.This study aimed to observe the therapeutic effect of AS-Ⅳon TON and the role of AMPK-mTOR-ULK pathway-mediated autophagy in this process.

The rats were divided into seven groups：sham operation group，model group，AS-Ⅳlow，medium and high dose groups，rapamycin group，AMPK inhibitor group（AS-Ⅳ+compound C）.Correlation tests were performed after 7 days of the corresponding drug intervention.

After 7 days，the phosphorylation levels of AMPK，mTOR and ULK proteins in retinal tissue changed significantly after TON.AS-Ⅳwas found to increase LC3 mRNA and protein levels in TON model rats in the study.In addition，it increased the levels of p-AMPK and p-ULK and reduced p-mTOR level.Moreover，AS-Ⅳincreased the survival rate of retinal ganglion cells（RGCs）and improved the P2 peak latency time of flash-visual evoked potential（F-VEP）.The above effects were reversed by the AMPK inhibitor compound C.

AS-Ⅳcan increase the survival rate of RGCs and improve visual function after TON，which is related to the improvement of autophagy by regulating the AMPK-mTOR-ULK pathway.The AMPK-mTOR-ULK pathway may be a potential therapeutic approach for TON.

To further verify the therapeutic effect of Liqi Tongbian Decoction（理气通便方）in treating slow transit constipation（STC）by regulating the intestinal microbiota/short-chain fatty acids（SCFAs）/5-hydroxytryptamine（5-HT）axis through fecal microbiota transplantation（FMT）technology.

After 7 days of adaptive feeding，24 rats were used in the experiment，of which 3 were the normal control group and received sterile water treatment.The rest 21 rats were used to establish the STC model through loperamide suspension+clamp tail stimulation and were treated with an antibiotic cocktail for 7 days to achieve decolonization.Among them，3 rats were randomly selected and euthanized to evaluate the intestinal motility.After successfully establishing the STCpseudogerm-free rat model，the animals were randomly divided into the normal rat fecal transplantation group，the STCrat fecal transplantation group and the Liqi Tongbian Decoction rat fecal transplantation group，with 6 rats in each group.All groups were given the corresponding rat fecal suspension daily by oral gavage for a week.After the gavage，the general condition and colonic tissue pathological changes of each group were observed.The intestinal microbiota and SCFAs in the feces of each group were detected.The concentration of 5-HT was measured by enzyme linked immunosorbent assay（ELISA），and the expression and distribution of 5-hydroxytryptamine receptor 3（5-HT3R）and 5-hydroxytryptamine receptor 4（5-HT4R）in colonic tissue were detected by immunohistochemistry.Additionally，the expression levels of tryptophan hydroxylase 1（TPH1），tryptophan hydroxylase 2（TPH2），5-hydroxytamine transporter（SERT）and monoamineoxidase A（MAO-A）in colonic tissue were detected by Western Blot.

The Liqi Tongbian Decoction rat fecal transplantation group significantly improved the water content of feces and increased the number of defecations，accelerated the colonic transit，increased the concentration of 5-HT in the serum，and increased the expressions of 5-HT3R and 5-HT4R in colonic tissue.It also increased the expression levels of TPH1，TPH2，SERT and MAO-A proteins.At the taxonomic level，significant differences were found in the genus Mucispirillum after fecal transplantation.The measurement of SCFAs showed that the fecal fluid of the rats in the Liqi Tongbian Decoction group had significantly up-regulated the level of butyric acid after treatment，while the other SCFAs did not show significant changes.

Liqi Tongbian Decoction can improve the structure of the intestinal microbiota in STCpseudogerm-free rats，up-regulate the abundance of Mucispirillum，mediate the production of SCFAs（especially butyric acid），stimulate the synthesis of 5-HT and the expressions of its receptors 5-HT3R and 5-HT4R，and promote the transport and metabolism of 5-HT，thereby improving the colonic transit function.These results further verify that regulating the intestinal microbiota/SCFAs/5-HT axis is a key step in the treatment of STCwith Liqi Tongbian Decoction.

To investigate the interventional effects of Buyang Huanwu Decoction（补阳还五汤）combined with Huanglian Jiedu Decoction（黄连解毒汤）（modified）on cognitive impairment in diabetic encephalopathy and explore its potential mechanisms through magnetic resonance spectroscopy（MRS）and metabolic indicators.

A prospective，randomized，controlled，double-blind study was conducted，enrolling119 patients with type 2 diabetes mellitus（T2DM）-associated cognitive impairment and Qi-Yin deficiency with phlegm-stasis syndrome from the Traditional Medicine Center of Jinhua Central Hospital between January 2024 and December 2024.The participants were randomized into an experimental group and a control group.The experimental group received Buyang Huanwu Decoction combined with Huanglian Jiedu Decoction（modified）combined with standard hypoglycemic therapy，while the control group received mecobalamin，piracetam and standard hypoglycemic therapy.The intervention lasted 12 weeks.Montreal Cognitive Assessment（MoCA）scores，hippocampal N-acetylaspartate/creatinine（NAA/Cr），frontal choline/creatinine（Cho/Cr）via MRSand serum metabolic indicators［glycosylated hemoglobin（HbA1c），homocysteine（Hcy），malondialdehyde（MDA）］were assessed at baseline，week 6 and week 12.Traditional Chinese Medicine（TCM）syndrome scores and adverse reactions were concurrently evaluated.

The experimental group showed a 15%～20% greater improvement in MoCA total scores compared to the control group，with significantly elevated hippocampal NAA/Cr（≥0.3）and reduced levels of Hcy and MDA（P<0.05）.Buyang Huanwu Deoction and Huanglian Jiedu Decoction（modified）demonstrated neuroprotective effects by improving neuronal metabolic status，regulating methionine pathways and mitigating oxidative stress.Regarding TCM syndrome scores，both primary and secondary symptom scores in the treatment group showed significant reductions，with an overall syndrome remission rate of 92.3%，which was significantly higher than that of the control group（P<0.001）.The degree of improvement was strongly correlated with the increase in MMSE scores（r＝-0.82，P<0.001）.No serious adverse events were observed in either group during the treatment period，and the treatment group demonstrated good safety.

Buyang Huanwu Decoction combined with Huanglian Jiedu Decoction（modified）exhibits favorable safety and cognitive improvement，potentially alleviating diabetic encephalopathy progression through modulation of neuro-metabolic homeostasis and systemic oxidative pathways，providing theoretical and mechanistic support for integrated TCM-Western medicine interventions.

To establish the preparation process，high performance liquid chromatography（HPLC）fingerprint and content determination method of the standard decoction of the stem of Feilongzhangxue［Toddalia asiatica（Linn）Lam］，and to analyze and evaluate the quality of decoction pieces by chemical pattern recognition method，so as to provide a reference for its quality control.To study the changes of the fingerprint of the stem of Feilongzhangxue［Toddalia asiatica（Linn）Lam］decoction piece and standard decoction，and to analyze the transmission law of the Toddalolactone in the decoction pieces-standard decoction.

HPLCfingerprints were established，and cluster analysis and principal component analysis were performed.The response surface method combined with principal component analysis（PCA）was used to evaluate the relative peak area and transfer rate of the eight common peaks，and the optimal decoction process was selected.The content of Toddalolactone in decoction pieces and standard decoction was determined by HPLC，and the transfer rate was calculated.

The similarity of the fingerprints of the stem of Feilongzhangxue［Toddalia asiatica（Linn）Lam］decoction pieces and the standard decoction was 0.719-0.996 and 0.992-1.000，respectively.Eight of the eleven common peaks in the decoction pieces were transferred to the standard decoction，and five of the common peaks were confirmed by comparison with the control substance，which were chlorogenic acid（peak 1），magnolidine（peak 2），hesperidin（peak 4），toddalolactone（peak 6）and toddaculin（peak 11）.The average ointment yield of 15 batches of standard decoction was9.29%，and the average transfer rate of Toddalolactone from decoction piece to standard decoction was 58.98%.

The fingerprint and content determination methods are stable and reliable，and the results of the transmission of the amount of Toddalolactone from decoction pieces to standard decoction indicate that the preparation process is scientific and feasible，which can provide a reference for its quality control.

To investigate the regulatory effects of Chaihu Shugan Powder（柴胡疏肝散）on the adenylate-activated protein activating enzyme（AMPK）/mammalian target of rapamycin（mTOR）/UNC-51-like kinase 1（ULK1）pathway and glial cells in the orbitofrontal cortex（OFC）of depression model rats.

The rat model of depression was established by chronic unpredictable mild stress（CUMS）.According to the random number table，10 rats were selected as the normal group and 50 rats with successful modeling were divided into model group，escitalopram group（10.50 mg/kg），Chaihu Shugan Powder lowdose group（3.15 g/kg），Chaihu Shugan Powder middle-dose group（6.30 g/kg），Chaihu Shugan Powder high-dose group（12.60 g/kg）according to the random number table，with ten rats in each group for 28 consecutive days.The changes of sugar preference coefficient，suspension immobility time and open field walking distance of rats in each group were observed.After the above detection was completed，the head was decapitated and the brain was removed，and the expressions of ionized calciumbinding receptor molecule 1（Iba-1），myelin basic protein（MBP）and glial fibrillary acidic protein（GFAP）in rat OFC tissues were detected by immunofluorescence assay.Western blotting method was used to detect the protein expressions of pT172-AMPK/AMPK，pS2488-mTOR/mTOR and pS757-ULK1/ULK1 in OFC tissue.The expressions of AMPK，mTOR and ULK1 mRNA in OFC were detected by real-time PCR.

Compared to the control group，the model group and each treatment group showed a decrease in sucrose preference coefficient，an increase in immobility time in the forced swimming test and a decrease in distance traveled in the open field test（P<0.05，P<0.01）.Compared to the model group，each treatment group showed an increase in sucrose preference coefficient，a decrease in immobility time and an increase in distance traveled in the open field test（P<0.01）.Compared to the control group，the model group and each treatment group showed an increase in protein expressions of Iba-1 and GFAP（P<0.05，P<0.01），and a decrease in MBP protein expression（P<0.01），except those in the Chaihu Shugan Powder low-dose group.Compared to the model group，each treatment group showed a decrease in protein expressions of Iba-1 and GFAP（P<0.01），and an increase in MBP protein expression（P<0.01）.Compared to the control group，the model group and each treatment group showed a decrease in protein expressions of pT172-AMPK/AMPK，pS2448-mTOR/mTOR and pS757-ULK1/ULK1（P<0.05，P<0.01）.Compared to the model group，each treatment group showed an increase in protein expressions of pT172-AMPK/AMPK，pS2448-mTOR/mTOR and pS757-ULK1/ULK1（P<0.05）.Compared to the control group，the model group，escitalopram group，Chaihu Shugan Powder low-dose group and middle-dose group showed a decrease in expressions of mTOR and ULK1 mRNA（P<0.01），Chaihu Shugan Powder low-dose group showed a decrease in AMPK mRNA expression（P<0.05），and the Chaihu Shugan Powder high-dose group group showed a decrease in mTOR mRNA expression（P<0.01）.Compared to the model group，each treatment group showed an increase in expressions of AMPK mRNA，mTOR mRNA nd ULK1 mRNA（P<0.01）.

Chaihu Shugan Powder can reduce the expressions of Iba-1 and GFAP in OFC of rats，increase the expression of MBPand activate AMPK/mTOR/ULK1 pathway expression to exert the antidepressant effect.

To review the toxic components and their mechanisms of action in tobacco products and smoke，in order to better carry out the research on the diseases caused by tobacco toxin.

The literature on cell culture，animal models and human exposure to tobacco toxicity was reviewed，compared and analyzed，and their literature was summarized and reviewed.

Tobacco smoke contains a variety of toxic components，including nicotine，carbon monoxide，amines，phenols，alkanes，aldehydes，polycyclic aromatic hydrocarbons，nitrogen oxides and heavy metals，and has a significant promoting effect on a variety of cancers，including lung cancer，pancreatic cancer，ovarian cancer，bladder cancer，cardiovascular and cerebrovascular diseases.In addition，the mechanisms of action of diseases caused by tobacco toxicity are diverse，mainly involving increasing the level of oxidative stress，promoting the production of DNA adducts，inducing chemokines，and affecting various pathways mediated by nicotinic acetylcholine receptor（nAChR）.

The tobacco epidemic is one of the greatest public health threats that the world is facing to date，killing many people each year.Tobacco toxicity in the etiology of traditional Chinese medicine，that is，the smoke produced by the combustion of tobacco inhaled by the human body，is a kind of toxin that has the characteristics of dry heat.At present，there is a wide range of research on carcinogens in tobacco toxin.However，there are relatively few studies on the diseases caused by exposure to tobacco toxins from multiple systems in the human body.Therefore，the carcinogenic components of tobacco toxicity should be clarified，and its mechanism of action should be explored，in order to expand the scope of etiological research in traditional Chinese medicine.

To observe the effect of the“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture technique on key proteins involved in endoplasmic reticulum stress（ERS）pathway apoptosis in ovarian tissue of poor ovarian response（POR）mice and to explore the possible mechanism of acupuncture improving ovarian function in POR mice.

According to the random number table method，45 mice with normal estrous cycles were selected and divided into a blank group，a model group and an acupuncture group（n＝15）.The blank group was orally administered with an equal volume of 0.9% sodium chloride solution，while the model group and acupuncture group were orally administered with Leigongteng（Tripterygium wilfordii）suspension（50 mg·kg^-1·d^-1）for 14 consecutive days to prepare a POR mouse model.After the completion of modeling，the acupuncture group selected the“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture method for intervention treatment，once a day，20 minutes for each time.After 14 days of continuous treatment，the ovulation induction was carried out，and the next day the samples were taken out.The changes in mouse estrous cycle were observed by using vaginal smear method.The ovarian indices of mice in each group were compared.ELISA method was used to detect the levels of follicle stimulating hormone（FSH），luteinizing hormone（LH），estradiol（E2）and anti Mullerian hormone（AMH）in mouse serum.HE staining method was used to detect the pathological morphology of ovarian tissue.Transmission electron microscopy was used to detect the ultrastructure of ovarian tissue.TUNEL staining was used to detect the apoptosis rate of positive cells in ovarian tissue.Immunohistochemistry was used to detect the positive expression of glucose regulated protein 78（GRP78）in ovarian tissue.Fluorescence quantitative PCR and Western blot were used to measure the changes in mRNA and protein expressions of protein kinase R-like endoplasmic reticulum kinase（PERK），eukaryotic initiation factor 2α（eIF2α），activating transcription factor 4（ATF4），C/EBP cyclic adenosine monophosphate response element binding transcription factor homolog protein（CHOP）and cysteine protease（Caspase）-3.

Compared with the blank group，the model group had an increased rate of estrous cycle disorder（P<0.01）.The number of eggs retrieved，ovarian wet weight and ovarian index decreased to varying degrees（P<0.01）.The expression levels of FSH and LH in serum increased（P<0.01），while the expression levels of E2 and AMH decreased（P<0.01）.There was destruction of ovarian tissue morphology and increased number of atresia follicles.Under electron microscopy，follicles deformed，mitochondria swelled and shrank，and mitochondrial cristae disappearred.The area of ovarian cell apoptosis increased（P<0.01）.The positive expression of ovarian GRP78 increased（P<0.01）.Ovarian tissue PERK，eIF2α，Elevated mRNA and protein expression levels of ATF4，CHOP，and Caspase-3（P<0.01）.Compared with the model group，the rate of estrus cycle disorder in the acupuncture group mice decreased（P<0.01）.The number of retrieved eggs，wet weight of ovaries，and ovarian index increased（P<0.01，P<0.05）.The expression levels of FSH and LH in serum decreased（P<0.01），while the expression levels of AMH and E2 increased（P<0.01，P<0.05）.Improvement in ovarian tissue morphology and reduction of atresia follicles.Under electron microscopy，the number of mitochondria in follicles increased，and the structure，shape，and arrangement improved to varying degrees.The area of ovarian cell apoptosis decreased（P<0.01）.The positive expression of ovarian GRP78 decreased（P<0.01）.the mRNA and protein expression levels of PERK，eIF2α，ATF4，CHOP and Caspase-3 in the ovarian tissue all decreased to varying degrees（P<0.01，P<0.05）.

The“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture can improve ovarian function and reduce ovarian granulosa cell apoptosis in POR mice，and its mechanism may be related toregulating the key factors of endoplasmic reticulum apoptosis pathway.

To evaluate the effect of Xinglou Chengqi Decoction（星蒌承气汤）in improving the intestinal microecology of acute cerebral infarction patients with phlegm-heat in Fu-organs syndrome.

A total of 60 acute cerebral infarction patients with phlegm-heat in Fu-organs syndrome who received the corresponding treatment in the hospital during May 2020 to June 2023 were selected as the subjects of this experiment，and were randomly divided into two groups.Both groups were treated with conventional western medicine，including lowering blood lipids，blood pressure and blood sugar，controlling underlying diseases，antiplatelet aggregation and neuroprotection.On this basis，the control group（30 cases）used aspirin enteric-coated tablets once a day and atorvastatin once a day orally.On the basis of conventional Western medicine treatment，the observation group（30 cases）was treated with Xingpu Chengqi Decoction，1 dose a day，taking twice in the morning and evening after decocting.Two groups were treated continuously for 14 days.The treatment efficacy of two groups was compared，and the improvement time of clinical symptoms such as limb weakness，aphasia，slurred speech and limb dysfunction before and after treatment was compared between two groups.Venous blood was collected on an empty stomach before and after treatment，and the levels of 5-hydroxytryptamine（5-HT），norepinephrine（NE），glutamicacid（Glu），doparnine（DA），brain-derived neurotrophic factor（BDNF）andγ-aminobutyric acid（GABA），the National Institutes of Health Stroke Scale（NIHSS）score，Barthel index score and modified Rankin score were compared between two groups before and after treatment.The numbers of bifidobacteria，lactobacillus，enterococci and yeast were compared between two groups before and after treatment.

Dopamine，neurotransmitters norepinephrine，epinephrine，5-hydroxytryptamine The total effective rate in the control group（63.33%，19/30）was significantly lower than that（86.67%，26/30）in the observation group.The improvement time of clinical symptoms such as limb weakness，aphasia，slurred speech and limb dysfunction in the observation group was shorter than that in the control group（P<0.05）.The levels of DA，NE，adrenaline，5-HT after treatment in two groups were decreased and the decrease in the observation group was higher than that of the control group（P<0.05）.The modified Rankin score and NIHSSscore in two groups were lower than those before treatment.Barthel index scores in two groups after treatment were higher than those before treatment.The improvement of the observation group was better than that of the control group（P<0.05），and the levels of bifidobacteria，lactobacillus and enterococci in two groups increased compared with those before treatment，and the levels of yeast decreased compared with those before treatment，and the improvement in the observation group was better than that of the control（P<0.05）.

Xinglou Chengqi Decoction can improve the nervous function of acute cerebral infarction patients with phlegm-heat in Fuorgans syndrome，reduce the clinical symptoms and improve the therapeutic effect.Its mechanism of action may be related to regulating the secretion of neurotransmitters and maintaining the balance of brain-gut axis.

To observe the clinical efficacy and safety of Xiangsha Wenzhong Decoction（香砂温中汤）combined with acupoint thread-embedding in the intervention for chronic atrophic gastritis（CAG）with Helicobacter pylori（HP）infection.

A total of 160 patients with CAG and Hp infection who were admitted to the hospital from January 2022 to June 2023 were enrolled in this study.They were randomly divided into group A，group B，group Cand group D using the random number table method.Group A was treated with acupoint thread-embedding.Group B was treated with Xiangsha Wenzhong Decoction.Group Cwas treated with acupoint thread-embedding combined with Xiangsha Wenzhong Decoction.Group D was treated with teprenone.The 4 groups were compared in terms of clinical efficacy，TCM syndrome scores，gastric mucosal histopathological score，endothelial function［epidermal growth factor（EGF）and vascular endothelial growth factor（VEGF）］，inflammatory factors［interleukin-6（IL-6）and tumor necrosis factor-α（TNF-α）］，cellular immune function，expression of cyclooxygenase-2（COX-2）and p53 in gastric mucosa，and the incidence of adverse reactions.

The total effective rate of group C（95.00%，38/40）was higher than that of group A，group B and group D［70.00%（28/40），75.00%（30/40）and 62.50%（25/40）］（P<0.05）.The total effective rates in group A，group Band group D were close（P>0.05）.After 6 months of treatment，TCM syndrome scores and gastric mucosal histopathological score of group Cwere lower than those of group A，group B and group D（P<0.05），but there was no significant difference among group A，group B and group D（P>0.05）.After 6 months of treatment，the levels of VEGF，CD3⁺and CD4⁺in group Cwere higher than those in group A，group B and group D.The levels of EGF，IL-6，TNF-αand CD8⁺were lower than those in group A，group B and group D（P<0.05）.However，there was no significant difference among group A，group B or group D（P>0.05）.After 6 months of treatment，the expressions of COX-2 and p53 in group C were lower than those in group A，group B and group D（P<0.05），but there was no significant difference among group A，group B and group D（P>0.05）.There was no significant difference in the incidence of adverse reactions among the four groups（P>0.05）.

Xiangsha Wenzhong Decoction combined with acupoint thread-embedding is significantly effective in the treatment of patients with CAG and Hp infection.It can improve the patients’symptoms and endothelial function，reduce the levels of inflammatory factors，enhance the immune function and down-regulate the expressions of COX-2 and p53，with high safety.

The objective of this study was to evaluate the clinical benefit of Modified Shenling Baizhu Powder（参苓白术散加减）in the treatment of acne.

From January 2020 to December 2022，120 patients with acne vulgaris were selected as participants in the Dermatology Outpatient Department of Lishui Hospital of Traditional Chinese Medicine.All patients were divided into two groups according to the difference of single and even numbers in the order of treatment.The group with single number of treatment was set as the control group，and the group with even number of treatment was set as the observation group.The control group was treated with isotretinoic acid softgel combined with fusidic acid cream，while the observation group was treated with Modified Shenling Baizhu Powder on the basis of the control group.

The effective rate of the observation group was 91.67%（55/60），which was significantly higher than that of the control group（78.33%，47/60）（P<0.05）.After 6 months of treatment，there were no significant changes in red blood cell count，white blood cell count，platelet count，liver and kidney function indexes（alanine aminotransferase，aspartate aminotransferase，serum creatinine and serum urea）between the two groups（P>0.05）.The amount of pimples，papules，pustules and total lesions in the observation group were significantly lower than those in the control group（P<0.05）.The satisfaction score of the patients in observation group was significantly higher than that in control group（P<0.05）.

For the patients with moderate and below acne vulgaris，Modified Shenling Baizhu Powder can not only improve the therapeutic effect，reduce the skin lesions，but also improve the satisfaction of the patients，and shows good safety performance.

To investigate the efficacy of Huadu Quxie Decoction（化毒祛邪汤）combined with CHOPchemotherapy in the treatment of lymphoma and its effect on cellular immune function.

A total of 102 patients with lymphoma treated in the hospital from January 2020 to December 2023 were selected as research objects，and the patients were divided into control group（51 cases）and observation group（51 cases）according to random number table method.The control group was treated with CHOP chemotherapy，and the observation group was treated with Huadu Quxie Decoction on the basis of the control group.Four weeks was a treatment cycle，and both groups received 4 cycles of treatment.After 4 cycles of treatment，the clinical efficacy of the two groups was compared，and the changes of cellular immune function（CD⁺₄，CD⁺₈，CD⁺₄/CD⁺₈），tumor markers［vascular endothelial growth factor（VEGF）and thymine deoxyribokinase（TK-1）］were compared before treatment and after 4 cycles of treatment，and the occurrence of adverse reactions during treatment was recorded.

The total effective rate of the observation group after treatment was significantly higher than that of the control group，and the difference was statistically significant（P<0.05）.The levels of cellular immune function indexes（CD⁺₄，CD⁺₈，CD⁺₄/CD⁺₈）in the observation group were significantly higher than those in the control group after treatment，and the difference were statistically significant（P<0.05）.The levels of VEGF and TK-1 in the observation group were significantly lower than those in the control group after treatment，and the difference were statistically significant（P<0.05）.There was no significant difference in the total incidence of adverse reactions between the two groups during treatment（P>0.05）.

Huadu Quxie Decoction combined with CHOP chemotherapy has significant efficacy in the treatment of lymphoma，and can improve the cellular immune function of patients and reduce the expressions of VEGF and TK-1 in serum，worthy of clinical promotion and application.

To investigate the effect of Danggui Shaoyao Powder（当归芍药散）combined with levofloxacin on clinical symptoms and matrix metalloproteinase-2/9（MMP-2/9）levels in patients with endometritis.

One hundred and twelve patients with endometritis in Reproductive Medicine Department of the hospital from January 2019 to January 2021 were enrolled and divided into two groups according to different treatment methods.The control group（52 cases）received levofloxacin therapy，while the observation group（60 cases）received Danggui Shaoyao Powder combined with levofloxacin.Then the comparison was conducted on serum inflammatory factors，MMP-2/9 levels，Visual Analogue Scale（VAS）score，Female Sexual Function Index（FSFI）score，sex hormone levels，clinical pregnancy outcome，the disappearance time of pelvic pain，leucorrhea and pelvic inflammation，the clinical efficacy and incidence of adverse reactions.

The levels of interleukin-4（IL-4），interleukin-6（IL-6），C-reactive protein（CRP），MMP-9 and MMP-2 at baseline yielded no statistical difference（P>0.05），while the above inflammation factors were significantly decreased in the observation group compared with those in the control group after treatment（P<0.05）.The scores of VASand FSFI yielded no statistical difference between two groups before treatment（P>0.05），while the observation group scored lower in VASand FSFI than the control group after treatment（P<0.05）.The disappearance time of pelvic pain，leucorrhea and pelvic inflammation was significantly shorted in the observation group compared with that in the control group（P<0.05）.The overall efficacy rate was 91.67%（55/60）in the observation group，which was significantly higher than 76.92%（40/52）in the control group，with statistical difference（χ²＝4.703，P=0.030）.The total adverse reaction rate was 5.00%（3/60）in the observation group，which was slightly lower than 7.69%（4/52）in the control group，with no statistical difference（χ²＝0.344，P=0.557）.The abortion rate was 0.00%（0/60）in the observation group，which was slightly lower than 1.92%（1/52）in the control group，with no statistical difference（χ²＝1.164，P=0.280）.The timing of pregnancy shortened in the observation group compared with that in the control group（P<0.05），and the clinical pregnancy rate was 83.00%（50/60）in the observation group，and was higher than 59.62%（31/52）in the control group，with statistical difference（χ²＝7.828，P=0.005）.The ectopic pregnancy rate was 0.00% in both groups，with no statistical difference.

The application of Danggui Shaoyao Powder combined with levofloxacin in the treatment of endometritis can effectively attenuate the inflammation response，down-regulate the serum levels of MMP-2/9，accelerate the disappearance time of pelvic pain and leucorrhea，reduce the levels of sex hormones，ameliorate the infertility symptoms，decrease the adverse reactions and improve the quality of life in patients.

To observe the clinical effect of Yiqi Jianpi Decoction（益气健脾方）in the treatment of spleen deficiency and phlegm obstruction type of children with adenoid hypertrophy（AH）.

From September 2022 to September 2023，70 children with spleen deficiency and phlegm obstruction type of AH treated in the hospital were selected and divided into Western medicine group and comprehensive group，with 35 cases in each，using a computer-generated random number table method.The western medicine group was treated with mometasone furoate nasal spray and montelukast sodium chewable tablets，while the comprehensive group was additionally treated with Yiqi Jianpi Decoction.The two groups were compared in terms of TCM syndrome integral，adenoid grading，treatment effects，quality of life and adverse reactions.

After treatment，the scores for snoring，nasal congestion，mouth breathing，apnea，pale complexion，loss of appetite and loose stool as well as the total scores were reduced in both groups（P<0.05），with the comprehensive group showing greater reduction than the western medicine group（P<0.05）.The proportion of grade1 adenoid grading in the comprehensive group increased compared to that in the western medicine group（P<0.05），while the proportion of grade 2 decreased（P<0.05）.The distribution of treatment effect grades and the overall effectiveness rate were higher in the comprehensive group than those in the western medicine group（P<0.05）.After treatment，the scores for sleep disturbance，physical symptoms，emotional symptoms，daytime function and impact on caregivers as well as total scores were reduced in both groups（P<0.05），with the comprehensive group showing greater reduction than the western medicine group（P<0.05）.There were no significant adverse reactions in either group，with one case of mild diarrhea in the comprehensive group that improved without treatment.

Yiqi Jianpi Decoction can alleviate symptoms of spleen deficiency and phlegm obstruction type of AH in children，improve the treatment effects and quality of life，and is highly safe.

To explore and analyze the potential quality markers（Q-markers）of Baizhi（Angelicae Dahuricae Radix）combined with fingerprint and network pharmacology methods based on the primary principles of Q-markers.

The similarity evaluation and common peaks identification were performed by establishing fingerprints of Baizhi（Angelicae Dahuricae Radix），and then the“active ingredient-target-pathway”network was constructed to predict the key targets and core components of Baizhi（Angelicae Dahuricae Radix）through the network pharmacology.Meanwhile，the integrative analysis of Q-markers of Baizhi（Angelicae Dahuricae Radix）from the perspectives of chemical composition validity，measurability and so forth based on current literatures.

Ten batches of fingerprints of Baizhi（Angelicae Dahuricae Radix）were established，10 common peaks were identified，and 4 peaks（byakangelicol，imperatorin，phellopterin，isoimperatorin）were identified by reference substances.In accordance with network analysis，the above four coumarins were its main bioactive compounds with high connectivity.Byakangelicol，imperatorin，phellopterin and isoimperatorin could be further clarified as the potential Q-markers of Baizhi（Angelicae Dahuricae Radix）according to the Q-marker concept.

The preliminary prediction and analysis of the Qmarkers of Baizhi（Angelicae Dahuricae Radix）provides a theoretical basis for further study on pharmacodynamics and pharmacological mechanism，which can militate in favor of whole quality control system of Baizhi（Angelicae Dahuricae Radix）.

To study the regulation of T helper cell 2（TH2）immune response in rats with allergic rhinitis（AR）by Ebushicao（Centipeda minima）extract via mediating Toll-like receptor-nuclear factor kappa B（TLR/NF-κB）signaling pathway.

ARmodels were induced with ovalbumin（OVA），and the rats were divided into model group，positive control group and Ebushicao（Centipeda minima）extract treatment groups（low，middle，high concentrations）.The rats in the positive control group were given loratadine（2 mg/kg）by gavage.The rats in the Ebushicao（Centipeda minima）extract treatment groups were given Ebushicao（Centipeda minima）extracts with the corresponding doses（2 mg/kg，4 mg/kg，8 mg/kg）by gavage，while those in the blank control and model groups were given an equal volume of saline by gavage.The treatment in all groups lasted for 10 days.Weights of nasal secretions were collected from the rats in each group and the scores for nasal symptoms were calculated.HE staining was used to observe the morphological changes of nasal mucosa，and the counts of eosinophils and neutrophils in nasal mucosa were calculated.The levels of interleukin-4（IL-4），interleukin-13（IL-13）and immunoglobulin E（IgE）in peripheral blood of rats in each group were detected by ELISA.The mRNA and protein expression levels of TLR-4 and NF-κB were detected by RT-PCR and Western-blot.In Ebushicao（Centipeda minima）extract treatment groups，the rats received natural TLR4 agonist lipopolysaccharide（LPS）and were observed for morphology of the nasal mucosa and nasal symptoms.

Compared with the blank control group，the rats in the model group had a significant increase in behavioral score of nasal symptoms，the levels of serum IL-4，IL-13 and IgE，the levels of eosinophil and neutrophi in nasal mucosal tissues，and the relative mRNA and protein expressions of TLR4 and NF-κB in nasal mucosal tissues，with statistical difference（P<0.05），while the above increases were notably diminished to varying degrees in the positive control group and Ebushicao（Centipeda minima）extract treatment groups（P<0.05）.Within Ebushicao（Centipeda minima）extract treatment groups，a gradual decline was observed in the above indexes as the concentration increased，with statistical difference in paired comparison（all P<0.05）.HE staining showed that the nasal mucosa of the control rats was normal pseudostratified ciliated columnar epithelium with intact mucosal epithelial structure and there was no obvious inflammatory cell infiltration.The epithelium of the nasal mucosa of the rats in the model group was heavily detached，and a large number of eosinophils and mast cells were observed in the lamina propria of the mucosa.Various degrees of pathological changes were also found in positive control group and Ebushicao（Centipeda minima）extract treatment groups，while the changes were remarkably attenuated compared with those in the model group.The weakening of pathological changes showed a concentration dependent manner within Ebushicao（Centipeda minima）extract treatment groups.After TLR4 agonist treatment，the improved AR symptoms and pathological changes in Ebushicao（Centipeda minima）extract treatment groups were reversed.

The mechanism of Ebushicao（Centipeda minima）extract in the treatment of AR may be related to the inhibition of TLR/NF-κB signaling pathway and alleviation of TH2 immune response.

To investigate the effects of Yiqi Yangrong Decoction（益气养荣汤）combined with acupuncture on T lymphocyte subpopulation and serum levels of granin A（CGA），soluble intercellular adhesion factor-1（slCAM-1）and human growth differentiation factor 3（GDF3）in patients with syndrome of both Qi and blood deficiency after breast cancer surgery.

Ninety-two patients with syndrome of both Qi and blood deficiency after breast cancer surgery were treated in the hospital from May 2020 to May 2023.They were divided into the control group and the test group，46 cases in each group，using random number table method.The control group was treated with CEF（Cyclophosphamide，Epirubicin，Fluorouracil）chemotherapy，and the test group was treated with Yiqi Yangrong Decoction combined with acupuncture and moxibustion on the basis of CEF chemotherapy，21 days for 1 cycle，a total of 4 cycles.The quality of life，the serum levels of CGA，slCAM-1，GDF3 and T lymphocyte subsets of the two groups before and after 4 cycles of treatment，the efficacy after 4 cycles of treatment，and the occurrence of adverse reactions during treatment were compared.

Compared with those before treatment，after 4 cycles of treatment，the functional dimension and overall quality of life scores of the two groups were increased，and those of the test group were significantly higher（P<0.05）.Compared with those before treatment，the symptom dimension scores/serum levels of CGA，slCAM-1 and GDF3 were decreased after 4 cycles of treatment，and the scores and levels were significantly lower in the test group（P<0.05）.Compared with those before treatment，the levels of CD4⁺and CD4⁺/CD8⁺in whole blood of the test group were increased after 4 cycles of treatment，and those of the control group were decreased.The levels were significantly higher in the test group（P<0.05）.The level of CD8⁺in whole blood of the test group decreased，and that of the control group increased.The level of CD8⁺in the test group was significantly lower（P<0.05）.The total effective rate of the test group was 83.33%（35/42），significantly higher than that of the control group（61.36%，27/44）after 4 cycles of treatment（P<0.05）.During the treatment period，no obvious adverse reactions occurred in the test group and the incidence of adverse reactions in the test group was significantly lower（P<0.05）.

Yiqi Yanggrong Decoction combined with acupuncture can regulate the levels of T lymphocyte subsets in patients with syndrome of both Qi and blood deficiency after breast cancer surgery，inhibit the serum levles of CGA，slCAM-1 and GDF3，control the abnormal proliferation of cancer cells，improve the quality of life，and reduce the risk of postoperative chemotherapy complications.

To analyze the effects of Qilong Capsule（芪龙胶囊）combined with edaravone dextrocamphorol and butylphthalein on serum amyloid protein（SAA），glial fibrillary acidic protein（GFAP），ubiquitin-carboxy-terminal hydrolase 1（UCH-L1）and nerve function in patients with acute cerebral infarction.

Ninety patients with acute cerebral infarction admitted to the hospital from202201 to 202401 were divided into the control group（45 cases）and the combination group（45 cases）by random number table method.The control group was treated with edaravone dextrocamphorol and butylphthalein，and the combined group was treated with Qilong Capsule combined with edaravone dextrocamphorol and butylphthalein for 2 weeks.Traditional Chinese medicine syndrome scores，serum levels of SAA，GFAP and UCH-L1，cerebral blood perfusion，neurological function before treatment，1 and 2 weeks after treatment，and the safety during treatment were compared between the two groups.

During the intervention period，2 cases fell off in the combined group and 1 case fell off in the control group.Compared with those before treatment，after 2 weeks of treatment，the scores of pale complexion，awkward speech，hemiplegia，shortness of breath and fatigue and the serum levels of SAA，GFAP and UCH-L1 in the two groups were lower and those in the combined group were significantly lower than those of the control group（P<0.05）.The National Institutes of Health Stroke Scale（NIHSS）score of the two groups showed a decreasing trend before treatment，1 and 2 weeks after treatment，and the score of the two groups was significantly lower in the combination group（P<0.05）.Compared with those before treatment，after 2 weeks of treatment，the end diastolic blood flow velocity（EDV），peak systolic flow velocity（PSV）and mean velocity（Vm）of middle cerebral artery in the two groups increased，and the levels were significantly higher in the combined group（P<0.05）.During treatment，none of the subjects stopped treatment due to adverse reactions，and the incidence of adverse reactions was similar between the two groups（P>0.05）.

Qilong Capsule combined with edaravone dextrocamphorol and butaphthalein can improve traditional Chinese medicine syndrome scores and nerve function in patients with acute cerebral infarction.The possible mechanism is to regulate the expressions of SAA，GFAPand UCH-L1，control the nerve cell injury and improve the cerebral blood perfusion，with good safety.

To explore the effects of Shuanglian Shecao Decoction（双莲蛇草汤）on pain and psychological status of patients with postherpetic neuralgia.

A total of 90 patients with postherpetic neuralgia admitted to the pain department from June12022 to December 12023 were selected and divided into two groups according to random number table method，with 45 cases in each group.The control group was given simple western medicine orally，and the experimental group was given Shuanglian Shecao Decoction on the basis of the control group.Ten days was a course of treatment，and there were a total of 3 courses of treatment.Pain scores，TCM symptom scores，negative emotion，quality of life and clinical efficacy were compared between the two groups.

Compared with those before treatment，the pain evaluation index（PRI），visual analog scale（VAS）score，present pain intensity（PPI）and the total pain score decreased in the two groups after treatment（P<0.05）.Compared with those of the control group，PRI，PPI，VASand total pain score of the experimental group were lower after 1 to 3 courses of treatment and decreased with the increase of treatment course（P<0.05）.Compared with those before treatment，TCM syndrome scores decreased and the score of the quality of life increased in both groups after treatment（P<0.05）.Compared with those of the control group，the TCM syndrome scores of the experimental groups were lower and the scores of the quality of life were higher after 1 to 3 courses of treatment（P<0.05）.With the increase of the treatment course，the TCM syndrome scores decreased，and the score of the quality of life increased（P<0.05），and the anxiety score and depression score decreased（P<0.05）compared with those before treatment.Compared with those in the control group，the scores of anxiety and depression in the experimental group were lower after 1 to 3 courses of treatment，and the scores of anxiety and depression decreased with the increase of treatment course（P<0.05）.Compared with those before treatment，the scores of compliance and satisfaction of the two groups were higher after treatment（P<0.05）.Compared with those of the control group，the scores of compliance and satisfaction of the experimental group were higher after 1 to 3 courses of treatment and increased with the increase of treatment course（P<0.05）.Compared with that of the control group，the effective rate of the experimental group was higher after 1 to 2 treatment courses（P<0.05）.There was no significant difference between the two groups after three courses of treatment（P>0.05）.

Shuanglian Shecao Decoction can improve the clinical treatment effect of sequelae，relieve the pain and other symptoms of patients.The patients recover quickly and the quality of life can be improved.It is worthy of promotion and application，and plays a good guiding role in the clinical treatment of postherpetic neuralgia.