Full
Outline
To establish the preparation process，high performance liquid chromatography（HPLC）fingerprint and content determination method of the standard decoction of the stem of Feilongzhangxue［Toddalia asiatica（Linn）Lam］，and to analyze and evaluate the quality of decoction pieces by chemical pattern recognition method，so as to provide a reference for its quality control.To study the changes of the fingerprint of the stem of Feilongzhangxue［Toddalia asiatica（Linn）Lam］decoction piece and standard decoction，and to analyze the transmission law of the Toddalolactone in the decoction pieces-standard decoction.
HPLCfingerprints were established，and cluster analysis and principal component analysis were performed.The response surface method combined with principal component analysis（PCA）was used to evaluate the relative peak area and transfer rate of the eight common peaks，and the optimal decoction process was selected.The content of Toddalolactone in decoction pieces and standard decoction was determined by HPLC，and the transfer rate was calculated.
The similarity of the fingerprints of the stem of Feilongzhangxue［Toddalia asiatica（Linn）Lam］decoction pieces and the standard decoction was 0.719-0.996 and 0.992-1.000，respectively.Eight of the eleven common peaks in the decoction pieces were transferred to the standard decoction，and five of the common peaks were confirmed by comparison with the control substance，which were chlorogenic acid（peak 1），magnolidine（peak 2），hesperidin（peak 4），toddalolactone（peak 6）and toddaculin（peak 11）.The average ointment yield of 15 batches of standard decoction was9.29%，and the average transfer rate of Toddalolactone from decoction piece to standard decoction was 58.98%.
The fingerprint and content determination methods are stable and reliable，and the results of the transmission of the amount of Toddalolactone from decoction pieces to standard decoction indicate that the preparation process is scientific and feasible，which can provide a reference for its quality control.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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