To observe the effect of Cangfu Daotan Decoction（苍附导痰汤）on the insulin receptor substrate-1（IRS-1）and glucose transporter type 4（GLUT4）signaling pathways in rats with polycystic ovary syndrome-insulin resistance（PCOS-IR）.

Sixty SPF-grade female SD rats were randomly divided into blank group，model group，Cangfu Daotan Decoction high-dose group，Cangfu Daotan Decoction low-dose group and metformin group，with 12 rats in each group.Except for the blank group，the other groups were induced PCOS-IR mdoel by subcutaneous injection of dehydroepiandrosterone（DHEA）combined with a high-fat diet for 20 days，while the blank group was injected with an equal volume of physiological saline.After the model was established，the rats in each group were administered the respective drugs by gavage for 28 days：metformin（0.265 g·kg^-1）and Cangfu Daotan Decoction（10.82，21.64 g·kg^-1）.The changes in body weight were recorded，and the samples were collected after the last administration.The intraperitoneal glucose tolerance test（IPGTT）was performed using a blood glucose meter to calculate the homeostatic model assessment of insulin resistance（HOMA-IR）and the area under the glucose curve（GAUC）.Hematoxylin-eosin（HE）staining was used to observe ovarian tissue under a light microscope and to count the numbers of primary follicles，secondary follicles and mature follicles.Enzyme-linked immunosorbent assay（ELISA）was used to measure serum levels of luteinizing hormone（LH），follicle-stimulating hormone（FSH），testosterone（T），estradiol（E2）and insulin（INS）.The protein expression levels of IRS1，phosphatidylinositol 3-hydroxy kinase（PI3K），serine/threonine kinase proteins（AKT）and GLUT4 in ovarian tissue were detected by Western blot.

Compared with the blank group，the model group had significantly higher body weight and levels of IPGTT，HOMA-IR and GAUC，significantly lower serum AMH levels，significantly higher levels of LH，FSH，T，E2 and INS，significantly fewer primary follicles，significantly more mature follicles，significantly higher IRS1 protein expression in ovarian tissue，and significantly lower protein expression levels of PI3K，AKT and GLUT4（P<0.05，P<0.01）.Compared with the model group，the Cangfu Daotan Decoction high-dose group and metformin group showed significantly lower body weight and levels of IPGTT，HOMA-IR and GAUC.The Cangfu Daotan Decoction high-dose group had significantly more primary follicles and significantly fewer mature follicles，while the metformin group and Cangfu Daotan Decoction high-dose group had significantly lower serum levels of E2，LH/FSH and T，significantly higher IRS1 protein expression in ovarian tissue，and significantly lower protein expression levels of PI3K，AKT and GLUT4（P<0.05，P<0.01）.Compared with the metformin group，the Cangfu Daotan Decoction high-dose group showed significantly lower levels of IPGTT，HOMA-IR and GAUC，significantly lower levels of LH/FSH and T，and significantly higher protein expression levels of PI3K，AKT and GLUT4 in ovarian tissue（P<0.05，P<0.01）.The result in the Cangfu Daotan Decoction high-dose group was closer to that in the blank group.

Cangfu Daotan Decoction significantly improves ovarian function in PCOS-IR rats，and its mechanism may be related to the inhibition of the IRS1/PI3K/AKT/GLUT4 signaling pathway.