Full
Outline
To explore the effects of ethanol extract from Tengligen（Radix Actinidiae Chinensis）on apoptosis and malignant biological behavior of non-small cell lung cancer.
The logarithmic growth stage non-small cell lung cancer cells A549 were selected to add different concentrations ethanol extract of Tengligen（Radix Actinidiae Chinensis）（0，10，20，40 μg/mL）.The proliferation inhibition rate of A549 cells was measured by using MTT assay.Annexin V-FITCwas used to measure of A549 cells apoptosis rate.Western Blot was used to determine the expressions of apoptotic proteins Bax and Bcl-2.The invasive ability of A549 cells was measured by using Transwell method.The scratch healing rate of A549 cells was measured by using scratch experiments.
The cell proliferation inhibition rates of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.The Bax grayscale values of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL，while the Bcl-2 grayscale values were lower than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and showed a dose-dependent effect.The cell invasion rate of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.The scratch healing rate of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the doses of 10，20 and 40μg/mL were higher than that of ethanol extract of Tengligen（Radix Actinidiae Chinensis）with the dose of 0μg/mL（P<0.05），and it showed a dose-dependent effect.
Ethanol extract of Tengligen（Radix Actinidiae Chinensis）can inhibit cell proliferation，invasion and migration，and induce apoptosis in non-small cell lung cancer A549 cells.It was related to Bax expression up-regulation and Bcl-2 expression down-regulation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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