ArchiveTo explore whether Chonglou（Rhizoma paridis）has a reversal effect on multidrug resistance of leukemia CEM/VCR and its reversal mechanism.
CEM/VCR cells were treated with different concentrations of Chonglou（Rhizoma paridis）for 72 hours，and the mortality rate was calculated，and the appropriate concentration of Chonglou（Rhizoma paridis）reversal agent was selected. MTT colorimetric experiment was used to calculate the inhibitory rate and half inhibitory concentration（IC50）of low，medium and high concentrations（0.1，0.5 and 2.5μg/mL）of vincristine（VCR）acting on Chonglou（Rhizoma paridis）treated cells（hereinafter referred to as the treated group）and unweighted Chonglou（Rhizoma paridis）treated cells（hereinafter referred to as the untreated group）for 24，48 and 72 hours respectively，and to calculate the reversal index（RI）and reversal multiple（IC50）between the two groups. Flow cytometry（FCM）was used to detect the apoptosis rate after VCR was applied to the two groups of cells. RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of Bax，Bcl-2，Cyt-c，P-gp and MRP1 after VCR acted on the two groups of cells.
The concentration of Chonglou（Rhizoma paridis）reversal agent was 0.008μg/mL. The inhibition rate of VCR in the treatment group was significantly higher than that in the untreated group（P<0.05 or P<0.01）. The IC50 value of VCR in the treatment group after 24，48 and 72 hours was reduced from 17.32，13.85 and 6.21μg/mL to 15.37，2.25 and 0.62μg/mL，and the reversal multiples were 1.12 respectively. The results of flow cytometry showed that the apoptosis rate of VCR group increased gradually with the increase of drug concentration，and the apoptosis rate of treated group was higher than that of untreated group under the same conditions. The results of RT-qPCR and Western blot showed that the expressions of Bcl-2，P-gp，MRP1-1 and Bax，Cyt-c genes and proteins were down-regulated and up-regulated by VCR with different concentrations at the same time compared with those of the control group（P<0.05），and this change was more significant in the treatment group.
Chonglou（Rhizoma paridis）can reverse the multidrug resistance of CEM/VCR cells，which may be related to down-regulating the expressions of P-gp and MRP1，reducing the efflux of chemotherapeutic drugs and increasing the intracellular concentration of VCR，down-regulating the expression of Bcl-2 and up-regulating the expressions of Bax and Cyt-c，inducing cell apoptosis.
To investigate the effect of Banxia Xiexin Decoction（半夏泻心汤）on Notch signaling pathway in Nmethyl-N'-nitro-N-nitrosoguanidine（MNNG）-induced gastric cancer model rats.
Fifty SD rats were randomly divided into control group，model group and Banxia Xiexin Decoction group. The control group was fed normally，and the model group and Banxia Xiexin Decoction group were treated with“MNNG gavage＋free drinking method”to induce gastric cancer model. The gastric pathological histology was observed by hematoxylin-eosin（HE）staining. Notch signaling pathway related protein expression was detected by Western Blot，and Notch signaling pathway related protein and Ki67 and VEGFA expressions were detected by immunohistochemistry method.
The incidence of tumor was 75.0%（6/8）in the model group and 33.3%（3/9）in the Banxia Xiexin Decoction group. Western Blot analysis showed that the expressions of Notch1，Jagged1，Dll4 and Hes1 in gastric tissue of rats in the model group were increased compared with those in the control group（P<0.05），and the above proteins were significantly decreased after Banxia Xiexin Decoction intervention compared with those in the model group（P<0.05）. The immunohistochemical results showed that the average optical density of Ki67，VEGFA，Notch1，Jaggedl，Dll4 and Hes1 in the model group was significantly higher than that in the control group（P<0.05），and the average optical density of these proteins after the intervention of Banxia Xiexin Decoction was significantly lower than that in the model group（P<0.05）.
Banxia Xiexin Decoction can intervene Notch signaling pathway，regulate the expressions of VEGF and Ki67，and inhibit the occurrence and development of gastric cancer to a certain extent.
Advanced heart failure with preserved ejection fraction（HFpEF）is a critical condition in the cardiovascular field，with limited treatment methods and poor prognosis. Recurrent wheezing and edema are the main clinical symptoms in the advanced HFpEF，which is classified as heart water disease according to traditional Chinese medicine（TCM）. The main treatment principle is to warm Yang and promote diuresis，which plays an important role in the treatment of advanced HFpEF. Based on the pathological characteristics of water retention in advanced HFpEF，the author systematically reviewed the etiology，pathogenesis，clinical manifestations，and characteristics of recurrent fluid retention，which is similar to the advanced HFpEF and can serve as the core pathogenesis of advanced HFpEF. Advanced HFpEF treatment in the stable and acute stages should base on the incubation and attack periods of recurrent fluid retention，using a combination of TCM and non pharmacological techniques to improve patients' clinical symptoms and enhance their quality of life，which will promote the ability and status of TCM in the treatment of critical illnesses by exerting multi-target integrated prevention and treatment advantages.
The key to achieving TCM syndrome differentiation and treatment through artificial intelligence lies in the standardization，normalization，dataization and intelligence of TCM four diagnostic methods. It analyzes the current status of artificial intelligence in the four examinations of inspection，listening and smelling，inquiry and pulse taking and palpation. Inspection adopts high-definition cameras to capture the entire face，tongue images，sublingual veins，lip color，eyes and other parts. The facial segmentation algorithm，color space model，spectral chromaticity and wavelength reflectance are combined to provide ideas for quantitative analysis of the observation. Inspection collects abnormal sounds such as cough and bowel sounds，and abnormal odors such as mouth，sputum and stool，and uses BP and SVM neural networks and electronic nose and other technologies for qualitative and quantitative analysis，providing a new method for the diagnosis of pulmonary and gastrointestinal diseases. Inquiries use the deep learning（DL）model for human-computer interaction to build a many-to-many probability map of pathogenesis and syndromes，providing probability statistics for accurate prescriptions. Pulse taking and palpation have been upgraded from a primary pressure sensor to a pulse wave touch sensor，which can collect three-dimensional pulse maps and dynamically detect blood flow imaging，diversifying the objective indicators of pulse diagnosis. There are still many problems to be solved urgently in the realization of artificial intelligence for the four diagnoses of traditional Chinese medicine：the standardization and quantification rules for the four diagnoses have not yet been improved；the hardware equipment for the individual collection of the four diagnoses is blooming in a hundred ways，but the hardware equipment for the combined collection of the four diagnoses is in its infancy，and the intelligence of the four diagnoses is low. The degree of integration is low；intelligent models，algorithms and big data are still immature in the field of medical video disease identification；big data information security risks lie，and there is a lack of crosscutting talents between medical and industrial departments to lead the development of the industry. This paper discussed and analyzed these issues.
This paper gave a detailed introduction to the application advantages and future development of deep learning in the objectification of the four diagnoses of traditional Chinese medicine，and explored the establishment of a new data integration collection processing idea based on deep learning in the objectification of the four diagnoses of traditional Chinese medicine. This paper summarized the research background of deep learning in the objectification of the four diagnoses of traditional Chinese medicine and the deep learning methods commonly used in the objectification of the four diagnoses of traditional Chinese medicine，and introduced the specific applications of deep learning in the objectification of the four diagnoses of traditional Chinese medicine in detail. The applications in inspection are divided into tongue diagnosis and face diagnosis，including tongue image data processing，tongue image data analysis，and face diagnosis data collection and analysis. In listening and smelling diagnosis，it is divided into the application of sound diagnosis and sniff diagnosis. It can be divided into the application of medical recommendation model and auxiliary diagnosis model. It is divided into the application of signal recognition and pulse classification，and the application of deep learning in traditional Chinese medicine diagnosis model and service platform，and discussed the application advantages and future prospects of deep learning in the objectification of traditional Chinese medicine four diagnoses. Based on deep learning，the integrated collection mode of objective data of the four diagnoses of traditional Chinese medicine is to classify the collected original four diagnoses data into the corresponding four diagnoses database after deep learning processing，and then output feedback with the exchange platform，and finally classify and apply.
To explore the inhibitory activity of phloretin on the RNA polymerase of the novel coronavirus，and predict the target enzyme inhibitory activity of three small analogs molecules.
Molecular docking was employed to investigate the binding mode of phloretin with the active site of RNA polymerase. The inhibitory activity of phloretin on RNA polymerase was evaluated using a cell-based assay system for the catalytic reaction of the novel coronavirus RNA polymerase nsp7/nsp8/nsp12. Three small molecules from traditional Chinese medicine which was similar to the structure of rhizobin were selected to predict the inhibitory activity of the targeted enzyme，and the binding mode between them and the target enzyme activity site was studied by molecular docking method.
Phloretin exhibited favorable binding to the active site of RNA polymerase，occupying the NTPs channel and obstructing the entry of nucleotide substrates，thereby inhibiting RNA polymerase-directed nucleic acid replication. The results of pharmacological testing for the inhibition of RNA polymerase at the cellular level demonstrated that phloretin possesses significant inhibitory activity against the novel coronavirus RNA polymerase，albeit with weaker activity compared to remdesivir. However，phloretin exhibited lower cytotoxicity compared to remdesivir. It is predicted that phloretin analogs can also inhibit RNA polymerase and have pharmacological effects against novel coronavirus.
This study reveals the RNA polymerase inhibitory activity of phloretin，and predict that the small molecules of the rhizetin analogs also have RNA polymerase inhibitory activity，providing a potential reference for the development of anti-novel coronavirus drugs.
To study the effect of Buyang Huanwu Decoction（补阳还五汤）combined with acupuncture on immune function and muscle strength recovery in patients with acquired myasthenia in Intensive Care Unit（ICU）.
From January to October 2023，72 patients with ICU-acquired myasthenia in the hospital were selected. The random number table method was divided into two groups，36 cases in each group. Both groups received routine rehabilitation intervention. At the same time，the control group was treated with acupuncture and moxibustion，and the observation group was treated with Buyang Huanwu Decoction combined with acupuncture. The recovery time，muscle strength，daily activity ability，immune function index and adverse reactions were compared between the two groups.
The mechanical ventilation time，ICU hospitalization time and total hospitalization time in the observation group were shorter than those in the control group［（5.97±2.10）d，（16.32±3.77）d，（32.67±3.18）d vs（7.45±1.84）d，（18.90±4.06）d，（35.29±4.06）d］（P<0.05）. There was no significant difference in muscle strength，daily activity ability or immune function between the two groups before treatment（P>0.05）. At 2 and 4 weeks after treatment，the muscle strength rating scale（MRC）scores of the observation group were lower than those of the control group［（76.85±6.92）points，（86.90±5.83）points vs（72.03±5.11）points，（82.76±6.47）points］（P<0.05）. The Barthel index（MBI）score of the observation group was lower than that of the control group［（74.34±5.68）points，（90.25±5.36）points vs（68.21±6.10）points，（86.03±6.33）points］（P<0.05）. The levels of interleukin（IL）-6，IL-8，tumor necrosis factorα（TNF-α）and insulin-like growth factor 1（IGF-1）in the observation group were better than those in the control group［（22.51±2.64）pg/mL，（43.74±5.60）pg/mL，（29.49±2.85）pg/mL，（117.50±10.49）ng/mL vs（26.08±3.05）pg/mL，（47.09±6.11）pg/mL，（33.01±2.48）pg/mL，（105.63±11.20）ng/mL］（P<0.05）. The incidence of adverse reactions was 8.33% in the observation group and 13.89% in the control group（P>0.05）.
Buyang Huanwu Decoction combined with acupuncture is effective in the treatment of ICU acquired myasthenia. It can enhance muscle strength，improve daily living ability，regulate immune balance，and promote faster recovery of patients. It is a safe and reliable treatment plan.
Chronic atrophic gastritis is a modern medical concept，which is categorized in traditional Chinese medicine as“epigastric pain”，“stuffiness of stomach”，“epigastric upset”and other diseases. The disease has the characteristics of insidious onset，long course and complexity. Most of the patients in the clinic show deficiency in origin and excess in superficislity，mix of cold and heat，and the prolonged disease often damages the positive Qi. And the real evils such as dampness，heat，blood stasis and other excess evils block the meridians and depletion of nutrient-Yin，and the loss of nourishment in the gastric mucosa leads to atrophy. Professor YAN Qinghai points out that according to his many years of clinical experience，chronic atrophic gastritis is the syndrome of deficiency in origin and excess in superficislity. The deficiency is Qi deficiency of spleen and stomach，leading to inability to transport and transform. The excess in superficislity is damp-heat obstructing the middle Jiao，or liver depression and Qi stagnation，liver-stomach disharmony，and poor circulation of Qi and blood. In the treatment of chronic atrophic gastritis，chronic atrophic gastritis is mostly treated with the basic principle of strengthening spleen and nourishing Qi，supplemented by methods such as clearing heat and dampness，soothing liver and harmonizing stomach. Combined with YAN Qinghai's own clinical practice，he has proposed a comprehensive treatment approach that combines tonifying，dispelling and regulating methods. Strengthening spleen，tonifying Qi，harmonizing stomach and descending adverse Qi，and clearing away heat and dampness are the common and effective methods for treating chronic atrophic gastritis. In this regard，Prof. YAN Qinghai has proposed the combination of disease differentiation and syndrome differentiation，the combination of macro and micro perspectives，the combination of treatment and mind regulation. The treatment combines the methods of treating the root，dispelling evil and dampness，and harmonizing spleen and stomach qi and blood. The patients' symptoms improved well and the clinical outcome was more significant.
To observe the efficacy and mechanism of Zhibitai Capsules（脂必泰胶囊）in the treatment of patients with non-alcoholic fatty liver disease and chronic hepatitis B.
A random number grouping method was used to divide 100 patients with non-alcoholic fatty liver disease and hepatitis B who were hospitalized in the hospital from January 1，2022 to April 30，2023，into an observation group and a control group，with 50 patients in each group. The control group received tenofovir fumarate 25 mg once a day. Fifty cases in the observation group were given 0.24 g of Zhibitai Capsule twice a day for 2 months，during which both groups were given routine symptomatic treatment of western medicine. The liver function［alanine aminotransferase（ALT），asparagus cochinchinensis aminotransferase（AST），glutamyltransferase（γ-GT）］，blood lipids［total cholesterol（TC），triglycerides（TG），high-density lipoprotein（HDL-C），low-density lipoprotein（LDL-C）］，renal function［blood urea nitrogen（BUN），creatinine（Cr）］，HBV-DNA amount and changes in TCM symptom scores of the two groups were compared before and after treatment.
The trial has concluded with 10 ineffective patients in the control group and 3 ineffective patients in the observation group. The total effective rate of the observation group was 94%（47/50），which was higher than 80%（40/50）of the control group. Compared with those before treatment，the liver and kidney function，blood lipids and traditional Chinese medicine syndrome scores of HBV-DNA in both groups improved compared to those before treatment. The improvement of ALT，AST andγ-GT of the observation group was better than that of the control group（P<0.05）. The reduction degree of TC，TG and LDL-C was higher than that of the control group，and the improvement degree of HDL-Cwas better（P<0.05）. The TCM syndrome score significantly improved（P<0.05）.
Zhibitai Capsules can effectively improve the blood lipid level，liver and kidney functions，assist in inhibiting the replication of HBV virus，delay the progression of the disease，and has a high factor of safety，which can provide an effective reference for the development of clinical treatment plans for the disease.
To investigate the effect of Linggui Zhugan Decoction（苓桂术甘汤）on lipid metabolism in mice with non-alcoholic fatty liver disease（NAFLD），and to provide a new therapeutic strategy and experimental basis for clinical treatment of NAFLD.
C57BL/6Jmice were used as blank control group. ApoE-/-mice were divided into model group and Linggui Zhugan Decoction low，middle and high dose groups. The blood lipid levels of mice were detected by automatic biochemical analyzer. HE staining and oil red O staining were used to determine the degree of liver steatosis in NAFLD mice. Free fatty acids（FFA）and triglyceride（TG）kits were used to detect the changes of liver FFA and TG levels in each group. RT-qPCR was used to detect the expressions of miR-125a-5p，estrogen receptor-related receptorsα（ERRα），carnitine palmitoyltransferase1C（CPT1C）and ACCmRNA. Western Blot was used to detect the protein expressions of ERRα，CPT1Cand ACC.
After treatment with Linggui Zhugan Decoction，the serum levels of total cholesterol（TC），TG and low density lipoprotein cholesterol（LDL-C）were down-regulated（P<0.05）. The levels of FFA and TGin liver of each group were down-regulated（P<0.05）. The expression of miR-125a-5p mRNA was increased（P<0.01），and the expression of ERRα，CPT1Cand ACCmRNA were decreased（P<0.01）. The protein expressions of ERRα，CPT1C and ACC were decreased（P<0.05）.
-Linggui Zhugan Decoction may prevent and treat NAFLD by interfering with miR-125a/ERRa/CPT1C pathway to affect fatty acid decomposition.
To observe the effect of Astragalus Polysaccharide Injection combined with intensity modulated conformal radiotherapy in the treatment of advanced cervical cancer.
A total of 82 patients with advanced cervical cancer admitted from February 2021 to December 2023 were included in this study. The patients were divided into two groups by random number table method，namely radiotherapy group and Chinese patent medicine group，with 41 cases in each group. All patients received conventional chemotherapy treatment，while the patients in the radiotherapy group also received intensity-modulated radiotherapy treatment. Those in the Chinese patent medicine group were treated with Astragalus Polysaccharide Injection on the basis of radiotherapy group，and the data of patients in each group was observed and compared，including the efficacy and adverse reactions，the changes of TCM symptom scores（hypoabdominal distention，weakness，shortness of breath，pale complexion，etc.）before and after treatment，squamous cell cancer（SCC），the changes in the levels of tumor markers such as carbohydrate antigen 125（CA125）and carcinoembryonic antigen（CEA），the changes in immune function indicators such as helper T cells，cytotoxic T cells，helper T cells/cytotoxic T cells，and EORTC Quality of Life scale（European Organization for Research and Treatment of Cancer）Cancer Quality of Life Questionnaire，EORTCQLQ-C30）score changes.
The total effective rate in the Chinese patent medicine group was significantly higher than that in the radiotherapy group（P<0.05）. The decrease rate of leukocyte in the Chinese patent medicine group was lower than that in the radiotherapy group（P<0.05）. The incidence of other adverse reactions was similar between the two groups（P>0.05）. The comparison of TCM syndrome scores（hypoabdominal distention，weakness，shortness of breath，pale complexion，etc.），tumor marker levels（CA125 and CEA），helper T cells，cytotoxic T cells，helper T cells/cytotoxic T cells，EORTCQLQ-C30 score and other indicators in both groups before treatment was not significant（P>0.05）. After treatment，TCM syndrome scores（hypoabdominal distention，weakness，shortness of breath，pale complexion，etc.）and tumor marker levels（SCC，CA125 and CEA）were all decreased in both groups，and EORTC QLQ-C30 scores were all increased，and the decrease and improvement degree of indexes in the Chinese patent medicine group were significantly higher than those in the radiotherapy group（P<0.05）. After treatment，helper T cells decreased，cytotoxic T cells increased，and helper T cells/cytotoxic T cells decreased in both groups. The degree of decline and increase of various indexes in the Chinese patent medicine group was lower than that in the radiotherapy group（P<0.05）.
Polysaccharide Injection combined with intensity modulated conformal radiotherapy has a good effect in the treatment of advanced cervical cancer，which can effectively reduce tumor markers，improve disease conditions and enhance immune function and quality of life of patients. The treatment is tolerable and relatively safe and feasible.
To analyze a randomized controlled study on the treatment of goiter patients with Modified Haizao Yuhu Decoction（海藻玉壶汤）.
A hundred goiter patients admitted to Quzhou Hospital of Traditional Chinese Medicine from May 2021 to May 2024 were selected as the study objects. The patients were divided into two groups by random number table method. Fifty patients treated with Western medicine were established as the control group，and the other fifty patients treated with Modified Haizao Yuhu Decoction were established as the experimental group，all of whom were treated for 12 weeks. After treatment，the clinical effects of the two groups were observed and compared.
After treatment，the single symptom scores of both groups were decreased（P<0.05），and the score of the experimental group was more significantly decreased than that of the control group（P<0.05）. After treatment，the levels of free triiodothyronine（FT3）and free thyroid hormone（FT4）were increased in both groups，and the level of thyroid stimulating hormone（TSH）was decreased（P<0.05）. Compared with those in the control group，the levels of FT3 and FT4 were higher and the level of TSH was lower in the experimental group（P<0.05）. After treatment，the maximum diameter and volume of thyroid nodules in both groups were decreased（P<0.05），and the decrease was more significant in the experimental group compared with that in the control group（P<0.05）. After treatment，the levels of thyroid autoantibodies thyroid peroxidase antibody（TPOAb）and thyroglobulin antibody（TGAb）were decreased in both groups（P<0.05），and the levels in the experimental group were more significantly decreased than those in the control group（P<0.05）.
After the treatment of goiter patients with Modified Haizao Yuhu Decoction，it can effectively regulate the immunity and improve the clinical symptoms of patients with good results.
Based on the NOD like receptor heat protein domain associated protein 3（NLRP3）/cysteine containing aspartic protease 1（Caspase-1）/deskininoid D（GSDMD）signaling pathway，experimental autoimmune thyroiditis（EAT）model rats were used as the research subjects to explore the mechanism of action of Modified Xiaoyao Powder（逍遥散加味）in the treatment of Hashimoto's thyroiditis（HT）.
Forty SD rats were randomly assigned to a blank group（10 rats）and a model group（30 rats）. The EAT rat model was established by subcutaneous multi-point injection of pig thyroglobulin adjuvant immunoemulsion combined with drinking high iodine water. After modeling，the rats were randomly divided into a model group（10 rats），a Modified Xiaoyao Powder group（10 rats），and a selenium yeast group（10 rats）. The Modified Xiaoyao Powder group was given 7.65 g/（kg·d）Modified Xiaoyao Powder solution，the selenium yeast group was given 36μg/（kg·d）selenium yeast tablet aqueous solution，and the remaining two groups were given distilled water by gavage of equal volume. After 8 weeks，HE staining was used to observe the morphological structure of thyroid tissue in EAT rats. The ultrastructure of thyroid follicular epithelial cells in EAT rats was observed under transmission electron microscopy. Enzym-linkedimmunosorbent assay（ELISA）was used to detect the levels of serum thyroid peroxidase antibody（TPO Ab），thyroid globulin antibody（TgAb），interleukin-1β（IL-1β），and interleukin-18（IL-18）in EAT rats. Western Blot was used to detect the expressions of apoptosis related proteins in thyroid tissue cells of EAT rats.
①The results of HE staining and transmission electron microscopy showed that there was obvious pathological damage in the thyroid tissue，the membrane structure of thyroid follicular epithelial cells was blurred and damaged，the mitochondria were obviously swollen，the membrane structure was blurred and dissolved，and the rough endoplasmic reticulum was dilated. The degree of thyroid pathological damage，mitochondrial and endoplasmic reticulum damage in all treatment groups was reduced，and the degree of reduction was the most obvious in the Modified Xiaoyao Powder group.②ELISA showed that the serum levels of TgAb，TPOAb antibody，and serum levels of IL-1βand IL-18 in the model group were significantly higher than those in the blank group（P<0.01），and the serum levels of TPOAb，TgAb antibody，IL-1βand IL-18 in each treatment group were reduced（P<0.01，P<0.05），especially in the Modified Xiaoyao Powder group.③As shown by the expression level of pyroptosis-related proteins in each group by WB，the expression levels of NLRP3，GSDMD，Caspase-1 and apoptosis-associated speck-like protein containing a CARD（ASC）protein in the thyroid tissue of rats increased significantly（P<0.01），and the remaining treatment groups decreased significantly（P<0.01，P<0.05），with the decrease being the most obvious in the Modified Xiaoyao Powder group.
Modified Xiaoyao Powder alleviates the pathological damage of thyroid tissue，reduces the levels of TPOAb and TgAb antibodies，and inhibits the pyroptosis state，which may be related to the regulation of NLRP3/Caspase-1/GSDMD signaling pathway.
To analyze the effect of Modified Baitouwen Decoction（加味白头翁汤）on the level of autophagy protein in patients with liver cancer after surgery.
Ninety-two patients with liver cancer admitted to the Department of Hepatobiliary and pancreatic Surgery of Shanxi Cancer Hospital were included as the study objects，and were selected from March 2022 to September 2024. They were divided into two groups by random number table method. Forty-six patients in the control group received surgical treatment，and 46 patients in the observation group received combined treatment with Modified Baitouwen Decoction on the basis of the control group. The therapeutic effect，TCM symptom scores，liver function indexes，tumor markers and related autophagy protein level were compared between the two groups.
The objective remission rate and disease control rate in the observation group were 43.48%（20/46）and 93.48%（43/46），respectively，which were higher than 31.91%（15/46）and 73.91%（34/46）in the control group（P<0.05）. After treatment，the TCM symptom scores of the observation group were lower than those of the control group（P<0.05）. After treatment，the levels of alanine aminotransferase（ALT），aspartate aminotransferase（AST）and total bilirubin（TBIL）in the observation group were lower than those in the control group，and the level of albumin（ALB）was higher than that in the control group，with significant difference between two groups（P<0.05）. After treatment，the levels of alpha fetoprotein isoform3（AFP-L3），Golgi apparatus protein 73（GP73），alpha fetoprotein（AFP）and gamma glutamyltransferase（γ-GT）in the observation group were lower than those in the control group，and the positive rates of microtubule associated protein 1 light chain 3（LC3），Beclin-1 and mammalian target protein of rapamycin（mTOR）were lower than those in the control group，with significant differences between the two groups（P<0.05）.
Modified Baitouwen Decoction has a clear therapeutic effect on patients with liver cancer after surgery，which can alleviate the relevant symptoms of patients，protect liver function，reduce the levels of tumor markers，regulate the levels of LC3，Beclin-1 and mTOR，inhibit the proliferation of cancer cells and delay the development of disease，and has high application value.
To observe the effect of Shegan Mahuang Decoction（射干麻黄汤）on T lymphocyte subsets in upper and lower airways of airway hyperresponsiveness（AHR）mice，and analyze the biological basis of simultaneous treatment of nose and lung in AHR treatment.
Gammadelta（γδ）T cells were purified from beta T receptor knockout mice. AHR model mice sensitized with ovalbumin were randomly attributed into normal control group（Ctrl），AHRgroup（AHR），γδT cells adoptive immunization group（AIT），Shegan Mahuang Decoction group（SGMHD），Shegan Mahuang Decoction and adoptive immunization group（SMA）. AIT group was injectedγδT cells via tail vein daily，SGMHTgroup was given Shegan Mahuang Decoction daily by gavage，and SMA group was given dailyγδT cell tail vein injection combined with Shegan Mahuang Decoction by gavage for 7 days. T lymphocyte subsetsmarkers in nasal mucosa and lung of mice were detected，including interferon-γ（IFN-γ），interleukin-4（IL-4），retinoic acid related orphan receptorγt（RORγt），forkhead transcription factor P3（Fox P3）andγδTcell receptor（γδTCR）. The ratios of Th1，Th2，Th17，Treg andγδT cells，and the corresponding cytokines in peripheral blood were also measured.
The results showed that compared with those in the Ctrl group，the expressions of IFN-γ，IL-4，FoxP3，RORγt andγδTCR of nasal mucosa and lung were higher in AHR group，with higher proportions of Th2，Th17 andγδT cells，higher concentrations of IL-2，IL-4，IL-5 and IL-17，and lower concentrations of IL-10 and TGF-β. Compared with those in the AHR group，the expressions of IFN-γ，IL-4，FoxP3，RORγt andγδTCRof nasal mucosa were lower in the AIT，SGMHD and SMA groups，with lower levels of IL-4 andγδTCR in lung，higher ratio of Th1 and lower ratios of Th17 andγδT cells in peripheral blood. The levels of IL-2，IL-4，IL-5 and IL-17 of SGMHT and SMA groups were lower expressed，however the levels of IL-10 and TGF-βwere higher.
The study clarified the effect of Shegan Mahuang Decoction on AHR mice through simultaneous treatment of nose and lung. The underlying mechanism was related to the comprehensive regulation of T lymphocyte subsets. The study laid a foundation for revealing mechanisms of Shegan Mahuang Decoction in the treatment of AHR and promoting its clinical practice.
To investigate the effect of Kuntai Capsule（坤泰胶囊）pretreatment combined with different cryopreservation carriers on embryo transfer outcomes in patients with diminished ovarian reserve（DOR）.
A retrospective analysis was conducted on 120 DOR patients undergoing in vitro fertilization（IVF）-frozen embryo transfer（FET）at the Reproductive Medicine Center of Huizhou Central People’s Hospital from January 2021 to October 2023. The patients were divided into four groups based on intervention：Group A［n＝30，conventional vitrification carrier（Cryotop）without herbal pretreatment］，Group B（n＝30，cryotop with Kuntai Capsule pretreatment），Group C［n＝30，novel tubular cryopreservation carrier（Tube Cryodevice Tiny-B1）without herbal pretreatment］，Group D（n＝30，Tiny-B1 with Kuntai Capsule pretreatment）. Groups B and D received Kuntai Capsule for three menstrual cycles. The parameters including anti Mullerian hormone（AMH），estradiol（E2），serum follicle stimulating hormone（FSH），endometrial thickness（ET），total antral follicle count，oocytes retrieved，high-quality embryos，embryo survival rate，implantation rate，clinical pregnancy rate，biochemical pregnancy rate and maternal-neonatal outcomes were compared. The data was analyzed using SPSS26.0.
Baseline characteristics showed no significant differences among four groups（P>0.05）. After intervention，AMH level in Group D was significantly lower than those in Groups B and C（P＝0.012）；the E2 levels in Groups Cand D were higher than those in Groups A and B（P＝0.025）；the endometrial thickness in Group D exceeded that in the other groups（P<0.000 1）. Total antral follicles：the result of Group D was higher than that of Group A（35.7%，P＝0.003）and that of Group C（31.7%，P＝0.009）. Oocytes retrieved：the result of Group D surpassed that of Group A（P＝0.021）but showed no significant difference compared with that of Group B（P＝0.081）. High-quality embryos：the result of Group B increased by 34.2% compared with that of Group A（P＝0.016），while the result of Group D increased by 51.0% compared with that of Group C（P＝0.028）. Embryo survival rates showed no statistical difference（P>0.05），but implantation rates in Groups B and D were significantly higher than those in Groups A and C（P<0.05）. Clinical pregnancy rates in Groups B（70.00%）and D（73.33%）were significantly higher than those in Group A（50.00%）and Group C（53.33%）（P<0.05）. No differences in biochemical pregnancy rates were observed（P>0.05）. Live birth rates in Group D were markedly elevated compared with those in Group A（RR＝1.67，95% CI［1.12，2.47］，P＝0.004）. The live birth rates of Group B also improved compared with that of Group A（RR＝1.50，95% CI［1.02，2.21］，P＝0.021）. Neonatal preterm birth rates and birth weights showed no differences among four groups（P>0.05）.
Kuntai Capsule pretreatment improves ovarian function and endometrial receptivity in DORpatients，enhancing implantation and clinical pregnancy rates. Combined with the novel tubular cryopreservation carrier，Kuntai Capsule further increases oocyte yield，high-quality embryos and live birth rates.
To study the synergistic effect of Astragalus polysaccharide（APS）on chemotherapy for cervical cancer transplanted tumor in nude mice，and to analyze its mechanism.
Human cervical cancer Hela cells were cultured in vitro and inoculated into the subcutaneous area of the right back near the armpit of nude mice. The tumor-bearing mice were randomly divided into eight groups：blank group，chemotherapy group（cisplatin，DDP），APSlow dose group（50 mg/kg），APSmedium dose group（100 mg/kg），APShigh dose group（200 mg/kg），APSlow，medium and high dose＋DDPgroup（combination group）. The general condition and tumor growth of tumor-bearing nude mice were observed during treatment，the tumor growth inhibition rate was calculated，and the expressions of glucose-regulated protein 78（GRP78），C/EBP homologous protein（CHOP），Caspase-3 Cysteinyl aspartate specific protease 3），microtubule-associated protein light chain 3 beta typeⅡ（LC3-Ⅱ）proteins in immunohistochemical and western blot tumor tissues were detected.
Compared with DDP group，after APSintervention，the activity of nude mice increased，the glossiness of skin and hair increased，the degree of wasting improved significantly，and the body weight increased. The tumor mass of combination group decreased significantly（P<0.01）. The number of nuclei in the tumor tissue of the combination group significantly decreased，with a large number of nuclei reduced and fragmented. The expression levels of GRP78，CHOP，Caspase-3 and LC3-II in the combination group showed a dose-dependent increase trend（P<0.01）.
APScan alleviate endoplasmic reticulum stress（ERS），stimulate mitochondria autophagy，promote tumor cell apoptosis，reduce chemotherapy resistance of cervical cancer cells，and has a synergistic and toxic effect.
To explore the effect and mechanism of“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture on improving the reproductive function of poor ovarian response（POR）mice by regulating ovarian mitochondrial dysfunction.
The screened mice with regular estrous cycles were randomly divided into a blank group，a model group and an acupuncture group. The mice in the model and acupuncture groups were administered Tripterygium wilfordii polysaccharide suspension（50 mg·kg-1·d-1）by gavage for 2 weeks，while the blank group received an equivalent volume of 0.9% sodium chloride solution by gavage. After modeling，the mice in the acupuncture group received continuous acupuncture treatment using“Zhibian（BL 54）-to-Shuidao（ST 28）”acupuncture for 2 weeks，once a day，20 minutes for each time. Then the three groups simultaneously underwent ovulation induction，and the samples were collected on the next day. Vaginal smears were used to observe the changes in the estrous cycle of mice. The number of retrieved oocytes，ovarian wet weight and ovarian index were measured. The serum levels of anti-Müllerian hormone（AMH），follicle-stimulating hormone（FSH），estradiol（E2）and luteinizing hormone（LH）were detected using enzyme-linked immunosorbent assay（ELISA）. The histopathological and ultrastructural changes in the ovary were observed through hematoxylin-eosin（HE）staining and transmission electron microscopy. The fluorescence staining was employed to detect the changes in mitochondrial membrane permeability pore（MPTP）permeability in ovarian tissues. The real-time fluorescent quantitative PCR was used to assess the mRNA expression levels of nuclear respiratory factor 1（NRF1），peroxisome proliferator-activated receptor-γcoactivator-1α（PGC-1α）and mitochondrial transcription factor A（TFAM）in ovarian tissues. The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling（TUNEL）staining was conducted to determine the apoptotic rate of positive cells in ovarian tissues and Western blot analysis was utilized to quantify the relative protein expressions of B-cell lymphoma-2（BCL-2），BCL-2-associated X protein（BAX），cytochrome C（Cyt C），cysteine-aspartic acid protease（Caspase）-9 and Caspase-3 in ovarian tissues.
Compared with the blank control group，the model group showed a significantly increased rate of estrous cycle disorder（P<0.01）. The number of ovulations，ovarian wet weight and ovarian index decreased significantly（P<0.01）. The serum levels of FSH and LH increased（P<0.01），while the levels of AMH and E2 decreased（P<0.01）. The pathological morphology of ovarian tissues and internal mitochondrial structure deteriorated. Mitochondrial membrane permeability increased（P<0.01）. The mRNA expression levels of PGC-1α，NRF1 and TFAM decreased to varying degrees（P<0.01，P<0.05）. The apoptotic rate of positive cells increased（P<0.01）. The protein expression levels of BAX，Cyt C，Caspase-9 and Caspase-3 increased（P<0.01，P<0.05），while the protein expression level of BCL-2 decreased（P<0.01）. Compared with the model group，the acupuncture group exhibited a significantly reduced rate of estrous cycle disorder（P<0.01）. The number of ovulations，ovarian wet weight and ovarian index increased significantly（P<0.01）. The serum levels of FSH and LH decreased（P<0.01），while the levels of AMH and E2 increased（P<0.01）. The pathological morphology of ovarian tissues and internal mitochondrial structure improved. Mitochondrial membrane permeability decreased（P<0.01）. The mRNA expression levels of PGC-1α，NRF1 and TFAM increased（P<0.01，P<0.05）. The apoptotic rate of positive cells decreased（P<0.01）. The protein expression levels of BAX，Cyt C，Caspase-9 and Caspase-3 decreased（P<0.01，P<0.05），while the protein expression level of BCL-2 increased（P<0.01）.
The“Zhibian（BL54）-to-Shuidao（ST28）”acupuncture may improve the mitochondrial dysfunction in the ovaries of POR mice by regulating the key factors involved in mitochondrial biogenesis and the mitochondrial apoptosis pathway，thereby reducing granulosa cell apoptosis in the ovary and enhancing ovarian responsiveness.
To investigate the effects of Buzhong Yiqi Decoction（补中益气汤）on the immune imbalance of Thelper cell 17（Th17）/regulatory T cell（Treg）and the Notch1/ROR-γt/Foxp3 signaling pathway in mice with autoimmune thyroiditis（AIT）.
Eighty CBA/J mice were randomly divided into normal group（15 mice）and model group（65 mice）. PBS＋Freund's incomplete adjuvant（IFA）was used to establish the AIT model. The mice were randomly divided into model group，Buzhong Yiqi Decoction low-dose，medium-dose and high-dose groups（4.10，8.19，16.38 g·kg-1）and western medicine group（sodium selenite tablets，0.26 mg·kg-1），11 mice in each group. The mice in the normal group and the model group were given the same dose of normal saline by gavage once a day，every 6 days，and stopped for 1 day，for 6 weeks. Hematoxylin-eosin（HE）staining and scanning electron microscopy（SEM）were used to observe the morphological changes of thyroid. The serum levels of thyroglobulin antibody（TgAb），thyroid stimulating hormone（TSH），free thyroid hormone（FT4），interleukin（IL）-10，IL-17A and IL-23 were detected by enzyme-linked immunosorbent assay（ELISA）. The mRNA expressions of Notch1，Foxp3 and ROR-γt in thyroid tissues were detected by Real-Time PCR. Western blot（WB）was used to detect the protein levels of Notch1，Foxp3 and ROR-γt in thyroid tissue.
Compared with those in the normal group，the serum levels of TgAb，IL-17A and IL-23 in the model group were significantly increased，while the serum level of IL-10 was significantly decreased（P<0.01）. HE staining showed that the structure of thyroid follicles in the model group was destroyed，lymphocytes were infiltrated in large sheets，and a large number of dense and slender microvilli were observed under SEM. Compared with that in the model group，the serum level of TgAb in the drug treatment groups decreased，and the changes in the Buzhong Yiqi Decoction middledose and high-dose groups and the western medicine group were more significant（P<0.01）. The serum levels of IL-17A and IL-23 of Buzhong Yiqi Decoction low-dose group were decreased，and the serum levels of IL-10 elevated（P<0.05）. The serum levels of IL-17A and IL-23 were significantly decreased，and the serum level of IL-10 was significantly increased in the Buzhong Yiqi Decoction medium-dose and high-dose groups and the western medicine group（P<0.01）. The degree of destruction of follicular epithelial cells was mild，lymphocyte infiltration was improved，and the microvilli on the cell surface was significantly reduced in each treatment group. Compared with those of the normal group，the expression levels of Notch1 and ROR-γt mRNA and protein in the thyroid tissue of the model group were significantly increased，and the expression levels of Foxp3 mRNA and protein were significantly decreased（P<0.01）. Compared with those in the model group，the expression levels of ROR-γt and Notch1 mRNA and protein in the Buzhong Yiqi Decoction groups were decreased to varying degrees，and the expression levels of Foxp3 mRNA and protein were increased to varying degrees，and those in the Buzhong Yiqi Decoction highdose group was the most significant（P<0.01）.
Buzhong Yiqi Decoction can significantly repair the pathological changes of thyroid tissue and alleviate inflammatory damage in AIT mice，mainly by regulating the Notch1/ROR-γt/Foxp3 signaling pathway to balance the immune response and establish immune function.
Baizhi（Radix Angelicae Dahuricae）is widely used in clinical medicine. Its volatile oil is the key active ingredient. It is mainly composed of olefins，alcohols，ketones，esters，acids，aldehydes，alkanes，furans，indoles and other compounds. Baizhi（Radix Angelicae Dahuricae）contains a large amount of starch. It is easy to gelatinize when extracting the volatile oil，and it is easy to oxidize after long-term exposure. The contents of volatile oil in Baizhi（Radix Angelicae Dahuricae）can fluctuate due to regional differences，cultivation methods，processing methods，extraction processes，growth environment and other factors. The volatile oil of Baizhi（Radix Angelicae Dahuricae）has high medicinal value. It can be used for anti-inflammation，relieving pain，calming，anti-tumor，anti-allergy，whitening and anti-oxidation in clinic. The volatile oil components of Baizhi（Radix Angelicae Dahuricae）were systematically collected and summarized by consulting the relevant literature of volatile oil of Baizhi（Radix Angelicae Dahuricae）at home and abroad. The factors affecting the contents of volatile oil of Baizhi（Radix Angelicae Dahuricae）and their stability were analyzed，and the efficacy and mechanism of volatile oil of Baizhi（Radix Angelicae Dahuricae）were studied. The aim is to provide reference for further research and development and utilization of Baizhi（Radix Angelicae Dahuricae）and its volatile oil.
To observe the effects of acupuncture at the back-Shu and front-Mu acupoints of the spleen meridian combined with treadmill training on the expressions of mammalian target of rapamycin（mTOR），p70 ribosomal protein S6 kinase（p70S6K），paired box transcription factor 7（Pax7）and myogenic differentiation antigen（MyoD）in the atrophied gastrocnemius muscle of a rat model with sciatic nerve transection，and to explore the mechanism by which acupuncture at the spleen meridian acupoints combined with treadmill training delays denervation-induced muscle atrophy.
Thirty SD rats were randomly divided into normal group，model group，treadmill exercise group，acupuncture group and acupuncture combined with treadmill exercise group（combination group），with 6 rats in each group. Except for the normal group，the rats in the other groups were subjected to sciatic nerve transection to establish a model of denervation-induced muscle atrophy in the left hind limb. Three days after successful modeling，the treatment was initiated. The acupuncture group received acupuncture at Pishu（BL20）and Zhangmen（LR13）once a day，6 times per week. The treadmill exercise group underwent treadmill training once a day，6 times per week. The combination group received treadmill training followed by acupuncture treatment（the same method as the above）. The intervention lasted for 4 consecutive weeks. The gastrocnemius muscle wet weight ratio was calculated for each group. Hematoxylin and eosin（HE）staining was used to observe the cross-sectional area of gastrocnemius muscle fibers. Western blot and RTPCR methods were employed to detect the protein and gene expression levels of mTOR，p70S6K，Pax7 and MyoDin the gastrocnemius muscle of each group.
The wet weight ratio of the gastrocnemius muscle and the average cross-sectional area of gastrocnemius muscle cells in the model group were lower than those in the normal group（P<0.05）. The wet weight ratio of the gastrocnemius muscle and the average cross-sectional area of gastrocnemius muscle cells in the treadmill exercise group，acupuncture group and combination group were higher than those in the model group（P<0.05）. The wet weight ratio of the gastrocnemius muscle and the average cross-sectional area of gastrocnemius muscle cells in the combination group were higher than those in the acupuncture group and treadmill exercise group（P<0.05）. The expressions of mTOR，p70S6K，pax7 and Myod proteins and genes in the gastrocnemius muscle of the model group rats were lower than those in the normal group（P<0.05）. The expressions of mTOR，p70S6K，pax7 and Myod proteins and genes in the gastrocnemius muscle of the acupuncture group，treadmill exercise group and combination group were higher than those in the model group（P<0.05），and the expressions of mTOR，p70S6K，pax7 and Myod proteins and genes in the affected side gastrocnemius muscle of the combination group were higher than those in the acupuncture group and treadmill exercise group（P<0.05）.
Acupuncture at the back-Shu and front-Mu acupoints of the spleen meridian and treadmill training may promote the synthesis of skeletal muscle-related synthetic proteins and increase the differentiation levels of skeletal muscle satellite cells by enhancing the expressions of mTOR，p70S6K，pax7 and Myod proteins and genes，thereby delaying denervated muscular atrophy.
Diyu（Sanguisorbae Radix）is a traditional Chinese medicine，that cools blood to stop bleeding，and relieves toxin to heal sores. However，New Compilation of Materia Medica highlights its dual function of stop bleeding and replenishing blood. At the same time，the prescription books of the past dynasties also recorded the application of Diyu（Sanguisorbae Radix）in replenishing blood. Modern pharmacological studies have confirmed that Diyu（Sanguisorbae Radix）has the effect of increasing white blood cells，red blood cells and platelets. Among them，Diyu Shengbai Tablet（地榆升白片）which is the preparation only with Diyu（Sanguisorbae Radix）can significantly improve the clinical symptoms of patients by increasing the white blood cell count，restoring peripheral blood image and increasing the number of platelets. Triterpenoid saponins in Diyu（Sanguisorbae Radix）can up-regulate Extracellular Signal-Regulated Kinase（ERK）and express and activate Nucleotide-binding Oligomerization Domain-like Receptor（NOD）signaling pathway to promote bone marrow cell proliferation. Phenolic acids promote the proliferation and differentiation of megakaritic progenitors by up-regulating the expression of thrombopoietin（TPO）and tumor necrosis factor-alpha（TNF-α）. Although the blood-tonic effect of Diyu（Sanguisorbae Radix）has been preliminarily confirmed，the connotation of its blood-tonic effect was still fully clarified. To this end，this paper systematically combed the ancient and modern literature related to Diyu（Sanguisorbae Radix）from the Han Dynasty to the present，and analyzed the literature on the blood-tonic effect of Diyu（Sanguisorbae Radix）based on the records of traditional materia medica，prescription books，clinical applications and modern pharmacological research in order to further clarify the understanding of the blood-tonic effect of Diyu（Sanguisorbae Radix）and expand the clinical application of Diyu（Sanguisorbae Radix）in blood-tonic-related fields.
To explore the biological mechanism of Parkinson's disease（PD）of kidney essence deficiency syndrome from the perspective of glucose metabolism and the mechanism of intervention of Dihuang Yinzi（地黄饮子）in PD of kidney essence deficiency syndrome.
Fifty-five SPF grade male 12-week-old SAM-P8 mice were divided into model group，positive drug group，Dihuang Yinzi low，middle and high dose groups，with 11 mice in each group，and 11 SAM-R1 mice were as the normal group. The PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine（MPTP）（30 mg/kg，once a day）into SAM-P8 mice for 7 days，and the intervention lasted for 4 weeks. The general status indicators of mice was observed，and the fasting blood glucose levels and behavioral indicators in mice were measured. HE staining was used to observe the structural changes of mouse kidney and testis. Western blot（WB），immunohistochemistry and polymerase chain reaction（PCR）were used to detect the protein contents of glucose transporter 1（GLUT1），glucose transporter 3（GLUT3），glucose transporter 4（GLUT4），and glycogen synthase kinase 3β（GSK-3β）in the substantia nigra and striatum of mouse brain tissue.
In the open field experiment，compared with the normal group，the model group showed a decrease in total exercise distance（P<0.05）and an increase in latency（P<0.05）. Compared with that of the model group，the latency of mice in the Dihuang Yinzi low and high dose groups decreased（P<0.05）. The total distance of mice in the Dihuang Yinzi middle dose group increased（P<0.05）. In the pole climbing experiment，compared with the normal group，the model group showed an increase in pole climbing time（P<0.05）. Compared with the model group，the Dihuang Yinzi low and high groups showed a decrease in time（P<0.05）. Compared with those before administration，the Dihuang Yinzi low，middle and high dose groups showed a decrease in time（P<0.05）. In the gait experiment，compared with the normal group，the model group showed significant differences in right anterior contact area，left anterior propulsion time，right posterior propulsion index，left posterior support phase and right anterior support phase（P<0.01）. Compared with that in the model group，there was a significant difference in the right posterior propulsion index（P<0.01）in the Dihuang Yinzi low dose group. There were significant differences in the contact area of the right forelimb，right posterior propulsion index，left posterior support phase，and right anterior support phase in the Dihuang Yinzi middle dose group（P<0.01）. There were significant differences in the contact area of the right forelimb，left posterior support phase and right anterior support phase in the Dihuang Yinzi high dose group（P<0.01）. HE renal staining test results showed compared with the blank group，the model group showed pathological changes such as glomerular epithelial cell proliferation，increased lipid droplets，renal tubular dilation，increased cell debris，increased red blood cells，increased lymphocytes，increased fibroblasts and increased fibroblasts. Compared with the model group，the Dihuang Yinzi high dose group showed a decrease in lipid droplets，red blood cells and fibroblasts. The HE testicular staining results showed that compared with the blank group，the model group showed pathological changes such as vacuolization of the spermatogenic epithelium and decreased sperm count. Compared with the model group，the Dihuang Yinzi high dose group showed reduced vacuolization of the spermatogenic epithelium. WB detection results showed compared with the normal group，the model group showed a significant decrease in GLUT1 protein expression in the substantia nigra（P<0.01），and a decrease in protein expressions of GLUT3 and GLUT4（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed a significant increase in protein expressions of GLUT1 and GLUT4 protein expression（P<0.01，P<0.05）and GSK-3βprotein expression decreased（P<0.05）. Compared with the normal group，the protein expressions of GLUT1 and GLUT3 in the striatum of the model group decreased significantly（P<0.01），and the expression of GLUT4 protein decreased（P<0.05）. The protein expression of GSK-3βincreased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed an increase in protein expression of GLUT1，GLUT3 and GLUT4（P<0.05，P<0.01），and GSK-3βprotein expression decreased（P<0.05）. Real time fluorescence quantitative PCR detection results showed compared with the normal group，the mRNA expressions of GLUT1，GLUT3 and GLUT4 in the substantia nigra of the model group mice were significantly reduced（P<0.01），and GSK-3βexpression increased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed increased expressions of GLUT1，GLUT3 and GLUT4（P<0.05），and GSK-3βexpression decreased（P<0.05）. Compared with the normal group，the mRNA expressions of GLUT1 and GLUT3 in the striatum of the model group were significantly lower（P<0.01），the expression of GLUT4 was lower（P<0.05）and the expression of GSK-3βincreased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group showed increased expressions of GLUT1，GLUT3 and GLUT4（P<0.05），and GSK-3βexpression decreased（P<0.05）. Immunohistochemical test results showed compared with the normal group，the expressions of GLUT1 and GLUT4 in the substantia nigra of the model group decreased（P<0.05），and GSK-3βexpression increased（P<0.05）. Compared with the model group，the Dihuang Yinzi high dose group significantly increased the expression of GLUT1（P<0.01）. Compared with the normal group，the expressions of GLUT1 and GLUT4 in the striatum of the model group decreased significantly（P<0.01，P<0.05），respectively. Compared with the model group，the Dihuang Yinzi high dose group significantly increased the expressions of GLUT1，GLUT3 and GLUT4（P<0.01，P<0.05），and the expression of GSK-3βdecreased（P<0.05）.
1. Dihuang Yinzi can improve the motor speed，grip ability，limb coordination and exploration ability of PD mice with kidney essence deficiency syndrome.2. Dihuang Yinzi can reduce fasting blood glucose in mice with PD kidney essence deficiency syndrome，improve energy metabolism by correcting glucose metabolism.3. Dihuang Yinzi can increase the protein expressions of GLUT1 and GLUT4 in the substantia nigra of PD mice with kidney essence deficiency syndrome，and reduce the protein expression of GSK-3βin the substantia nigra of the brain，increase the expressions of GLUT1，GLUT3 and GLUT4 in the brain striatum，and reduce the expression of GSK-3β in the brain striatum. The protein expression in the Dihuang Yinzi middle and high dose groups was higher than that in the Dihuang Yinzi low dose group.4. Dihuang Yinzi has abnormal intake of GLUT1，GLUT3，GLUT4 and GSK-3βproteins. Dihuang Yinzi can treat PD of kidney essence deficiency syndrome by regulating the expressions of GLUT1，GLUT3，GLUT4 and GSK-3β proteins in the substantia nigra and striatum of PD mice.
Through a systematic review and comparison of relevant literature on the biosynthesis factors and extraction processes of flavonoids in medicinal plants，this paper systematically discussed the abiotic factors that affect the synthesis of flavonoids in medicinal plants，such as drought stress，salt stress and ultraviolet（UV）stress，as well as abiotic factors such as nematode invasion and pathogen infection. When dealing with drought stress，a large amount of flavonoids act as complex buffer solutions，continuously exported from the roots through biosynthesis and transformation，providing conditions for clearing reactive oxygen species and accurately and rapidly maintaining the balance of reactive oxygen species in the plant body，thus effectively responding to the ever-changing environment. Short-term salt stress alleviates cell membrane damage by activating antioxidant enzyme activity. UV radiation and light intensity promote the synthesis of flavonoids to achieve their stress resistance. In the face of biological stress，flavonoids，as plant specific antitoxin，can have a killing effect on invading organisms，thereby protecting plants. In addition，the extraction methods of flavonoids from medicinal plants，including ultrasonic-assisted extraction，microwave assisted extraction and enzyme extraction，were summarized. A systematic description was provided on the classification of solvents and their impact on extraction efficiency in order to provide some reference for the widespread application of flavonoids in medicinal plants. Although the stress has a certain promoting effect on the synthesis of flavonoids，the application of traditional Chinese medicine emphasizes integrity，and whether stress will damage the integrity of medicinal plants in therapeutic effects still needs to be explored.
To observe the effects of Tiaomo Decoction（调膜汤）combined with pelvic floor electric stimulation on endometrial thickness，endometrial shape and endometrial blood flow in patients with kidney deficiency and blood stasis syndrome of thin endometrial，and to evaluate its curative effect on thin endometrial.
A total of 90 patients with thin endometrium who were admitted to the Obstetrics and Gynecology Clinic of Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from October 2022 to October 2023 were selected and included into the control group，observation group 1 and observation group 2 according to their treatment intention，with 30 cases in each group. The control group was given low-dose aspirin orally during non-menstrual period，the observation group 1 was given Tiaomo Decoction orally from the first to the 28th day of menstruation on the basis of the control group，and the observation group 2 was given pelvic floor bioelectric stimulation treatment during non-menstrual period on the basis of observation group 1. One menstrual cycle was 1 course of treatment. After 3 courses of treatment，menstrual blood volume，traditional Chinese medicine（TCM）syndrome score，endometrial thickness，endometrial morphology and endometrial blood flow index were compared before and after treatment.
The total effective rate of the observation group 1 and 2 was better than that of the control group（P<0.05）. After treatment，the blood volume score，TCM syndrome score，intima thickness and type and hemodynamics of the observation group 1 and 2 were significantly improved compared with those before treatment（P<0.05），and the efficacy was better than that of control group（P<0.05）. The intima thickness，intima type A rate，blood flow pulsation index and blood flow resistance index of the observation group 2 were more significant than those of the observation group 1（P<0.05）.
Based on the therapeutic theory of“invigorating kidney Qi and regulating cell pulse”，Tiaomo Decoction combined with pelvic floor electrical stimulation can increase the menstrual blood volume of patients，improve the clinical symptoms，increase the endometrial thickness and the proportion of type A endometrial by improving the uterine blood microcirculation，induce endometrial growth and improve the receptivity of thin endometrial.
Increased resistance of Helicobacter pylori to antibiotics is the primary factor contributing to the failure of clinical eradication therapy. Resistance mechanisms include specific resistance to certain types of antibiotics and nonspecific resistance to external attack factors. The latter includes impaired drug uptake/excretory mechanisms，biofilm formation，spherical transformation，and internalization of Helicobacter pylori，all of which are significant in the development of multidrug resistance in Helicobacter pylori. The use of Chinese herbal medicines or their active ingredients to enhance Helicobacter pylori eradication rates，inhibit drug-resistant strains and increase sensitivity to antibiotics has garnered increased attention in eradication therapy. The pathways of action of these herbal medicines are primarily associated with nonspecific resistance mechanisms. This review focused on the nonspecific drug resistance of Helicobacter pylori and the anti-drug resistance mechanisms of traditional Chinese medicine to address the growing issue of antibiotic resistance in Helicobacter pylori，which holds great clinical significance.
By consulting relevant ancient and modern literature，the processing history，the modern processing，the changes of chemical composition and pharmacological effects after the processing of Baizhu（Atractylodesmacrocephala Koidz）were organized and summarized，providing a basis for the standardization of the development of Baizhu（Atractylodes macrocephala Koidz）processing products. According to the ancient records，the processing methods of Baizhu（Atractylodes macrocephala Koidz）include purification，cutting，frying，removing oil，stir-frying with auxiliary materials，cannoning，simmering，etc. Stir-frying is the most documented and well categorized，honey-processing and other stir-frying methods have been in use to the present day. Among them，Baizhu（Atractylodes macrocephala Koidz）processed by stir-frying method is most widely used in clinical practice. The chemical constituents of Baizhu（Atractylodes macrocephala Koidz）that have been documented in the current literature to change after processing include polysaccharides，lactones and volatile oils. The pharmacological effects of Baizhu（Atractylodesmacrocephala Koidz）processing products include gastrointestinal motility regulation，immunomodulation，antitumor，anti-inflammatory，regulation of glucose and lipid metabolism and hepatoprotection effects. However，there is a lack of research on the correlation between changes in content and pharmacological effects in the study of processing pharmacology. Future studies could be based on the analysis of the processing technology and the changes in component contents of Baizhu（Atractylodes macrocephala Koidz），to further explore the relationship between component changes and pharmacological effects and analyse the molecular mechanism of component contents in Baizhu（Atractylodes macrocephala Koidz）processing，with a view to laying the foundations of guidelines and pharmacological applications of Baizhu（Atractylodes macrocephala Koidz）decoction pieces.
To study the efficacy of Bushen Fangshu Decoction（补肾防疏汤）in the treatment of osteoporotic thoracolumbar compression fracture（kidney Qi deficiency syndrome）and its effect on fracture healing，and to analyze the related mechanism of action.
Ninety-eight patients with osteoporotic thoracolumbar compression fracture of kidney Qi deficiency syndrome who underwent surgical treatment in Weifang People's Hospital from January 2023 to January 2024 were included in the study，and they were divided into two groups by randomized numerical table method，49 cases of the control group were treated with conventional anti-osteoporosis therapy after surgery，and 49 cases of the observation group were treated with Bushen Fangshu Decoction on the basis of the control group，and the effect of the two groups was compared. The two groups were compared in terms of therapeutic effect，traditional Chinese medicine symptom scores，bone mineral density，lumbar spine function，pain level and bone metabolism index changes.
The total effective rate of the observation group was 95.92%，much higher than 77.55% of the control group，and the difference between the two groups was significant（P<0.05）. The points of the three main symptoms，lumbar spine function oswestry disability index（ODI）score，and fracture healing time of the observation group were lower than those of the control group（P<0.05）. The visual analog scale（VAS）scores of the observation group were lower than that of the control group in the 1-week，1-month，2-month and 3-month periods after surgery（P<0.05）. The levels of osteoporosis screening tool（OST），bone-specific alkaline phosphatase（BAP）and 25-hydroxyvitamin D［25（OH）D］and the bone mineral density of the observation group were higher than those of the control group after treatment（P<0.05）. VASscores at each time point were lower than those of the control group（P<0.05）. The levels of OST，BAP and 25（OH）D and the bone mineral density in the observation group were higher than those in the control group after treatment（P<0.05）.
The application of Bushen Fangshu Decoction for postoperative treatment of osteoporotic thoracolumbar compression fracture with kidney Qi deficiency syndrome can effectively reduce postoperative pain，improve bone density，promote bone metabolism，and has a positive effect on promoting functional recovery and improving quality of life for patients.
Erysipelas is a prevalent surgical complication with prolonged duration and propensity for recurrence. Traditional Chinese medicine offers a robust therapeutic approach to addressing this condition. FANG Zhixuan is a renowned traditional Chinese medicine surgical expert and a prominent figure in the field of Yanjing traditional Chinese medicine surgery. He is particularly known for his contributions to the“Fang's surgery”，which has become a hallmark of this surgical approach. Fang's Surgery is grounded in the academic traditions of the Yanjing Imperial School of Medicine and draws upon the insights of Golden Mirror of Medical Sect，which has made significant contributions to the treatment of breast diseases，skin diseases and peripheral vascular diseases. This article presented FANG Zhixuan's experience in the management of erysipelas，encompassing different aetiologies and pathogenesis，various stages of development，treatment approaches and a combination of internal and external medications. These were discussed in the context of integrating local and holistic diagnosis with internal and external treatments.
Mabiancao（Verbena officinalis L.）contains a variety of active ingredients such as flavonoids，iridoid terpenoids，phenylethanol glycosides，triterpenoids，sterols and volatile oils. Mabiancao（Verbena officinalis L.）has many pharmacological effects such as anti-inflammatory，analgesic，antitussive，anti-fertility，promoting milk secretion，and anti-tumor. Moreover，the resource of Mabiancao（Verbena officinalis L.）is abundant globally，and has broad application prospects. In this study，it systematically reviewed the current quality control status of Mabiancao（Verbena officinalis L.），and analyzed it from seven aspects including plant relative theory，compatibility of traditional Chinese medicine，traditional efficacy，modern pharmacological action，different producing areas，effectiveness and predictability of ingredients，based on the concept of quality marker（Q-Marker）. The results showed that the chemically active ingredients such as ursolic acid，oleanolic acid and verbenaglycosides can be used as QMarkers of Mabiancao（Verbena officinalis L.），which provided a good basis for the quality control research and establishment of quality standards for Mabiancao（Verbena officinalis L.）in the future.
The classic prescription Xiebai Powder（泻白散）was first recorded in QIAN Yi's Xiao'er Yaozheng Zhijue in the Northern Song Dynasty. It has the effects of clearing lung heat，relieving cough and relieving asthma. The chemical components of the whole prescription are diverse，mainly organic acids，flavonoids and alkaloids. Xiebai Powder has rich pharmacological effects such as eliminating phlegm，relieving cough and asthma，anti-inflammation，antipyretic and analgesic effect and anti-pathogenic microorganisms. Modern clinical treatment is mainly used to treat respiratory diseases such as pneumonia，chronic bronchitis，cough and asthma in children. It can also be used to treat skin diseases，lung cancer，rhinitis，functional constipation in children and other diseases. At the same time，based on the“five principles”of quality markers，the Quality markers（Q-Markers）of Xiebai Powder was predicted and analyzed. The results showed that morin C，mulberroside A，esculin，kaempferol，quercetin，glycyrrhizic acid，glycyrrhetinic acid and liquiritin were the Q-Markers of Xiebai Powder，in order to provide a more comprehensive reference for the establishment of a more scientific quality evaluation standard system of Xiebai Powder.
Henoch-Schonlein purpura is a common vascular inflammatory disease in childhood，which has the characteristics of high incidence，high recurrence rate and difficult course of disease. At present，although western medicine treatment can alleviate the disease，but long-term use has more adverse reactions. The pathogenesis is“heat，deficiency，stasis”，based on syndrome differentiation，with few adverse reactions and high patient compliance. Xijiao Dihuang Decoction（犀角地黄汤），which was first seen in Beiji Qianjin Yaofang，has the effect of clearing away heat and cooling blood，promoting blood circulation and removing blood stasis，which is suitable for this disease. By reviewing the treatment of Henoch-Schonlein purpura from the theory，the clinical application and mechanism of action，this paper aimed to provide reference for further research on the Henoch-Schonlein purpura.
To identify key genes，functional enrichment and signaling pathways associated with Parkinson's disease（PD）using bioinformatics methods.
The PD microarray dataset GSE7621 was downloaded from the Gene Expression Omnibus（GEO）database. Weighted Gene Co-expression Network Analysis（WGCNA）was employed to screen key gene modules，and the differentially expressed genes（DEGs）were identified using R software. Gene Ontology（GO）and Kyoto Encyclopedia of Genes and Genomes（KEGG）pathway analyses were conducted on the selected DEGs，and the key genes within the DEGs were identified using the STRING database and Cytoscape software.
A total of 133 key genes were identified through WGCNA and DEGscreening. GOand KEGGanalyses indicated that the primary biological processes involved ubiquitination and histone modification. The key genes included CUL7，KAT2A，DDB1，ANAPC7，ATXN7L2，TADA2B，SGF29，PSMB1，RSAD1 and MRPL30.
This study identified several key genes associated with PD through bioinformatics analysis，revealing biological processes and signaling pathways related to ubiquitination，histone modification and the Notch signaling pathway. These findings provide new insights into the molecular mechanisms of Parkinson's disease.
Shaoyao Decoction（芍药汤）is expected to be a candidate prescription for the treatment of colorectal cancer，but its underlying mechanism and molecular targets remain to be explored.
A mouse model of colorectal cancer transplantation was established，and 30 mice were divided into 3 groups：model group（M，n＝10），model＋Shaoyao Decoction group（SYD，n＝10）and model＋Shaoyao Decoction＋ROSclearance group（SNR，n＝10）. The mice of the SYD group were given 37 g/kg Shaoyao Decoction by gavage，the mice of the SNR group were given an equal amount of Shaoyao Decoction after intraperitoneal injection of ROSclearance agent，and those of the M group were given the same amount of distilled water. The drug was administered continuously for 21 days. The body weight and tumor volume of mice were observed，ROSlevels in tumors were detected by fluorescence method，and the protein expression in autophagy pathway was detected by western blot.
Shaoyao Decoction inhibited the growth of transplanted colorectal cancer in mice，induced ROSaccumulation in tumor tissues，up-regulated the expressions of Beclin-1，LC3，BIP，PERK and ATG5 proteins，down-regulated the expression of p62 protein，and inhibited the expressions of proteins in PI3K/AKT/mTOR signaling pathway.
Shaoyao Decoction can induce autophagy by promoting excessive accumulation of reactive oxygen species，thus inhibiting the growth of colorectal cancer transplant tumors.
Chronic atrophic gastritis（CAG）is a common disease of the digestive system，and its incidence ranks first among various gastric diseases，with epigastric fullness，pain，pantothenic acid，nausea and vomiting as the main clinical manifestations，and the occurrence of this disease is related to Helicobacter pylori（Hp）infection，dietary and environmental factors，autoimmunity，bile reflux，etc.，among which Hp infection is the most. At present，the treatment of CAGin Western medicine is mainly based on the symptomatic treatment of multi-drug combination，although it can achieve certain effects，but the adverse reactions are obvious，and long-term use is easy to develop drug resistance. Traditional Chinese medicine（TCM）has the advantages of multicomponent，multi-target and multi-channel，and has significant efficacy in the prevention and treatment of CAG. This article summarized and sorted out the theoretical basis and mechanism of Banxia Xingxin Decoction in the treatment of CAGby searching and reviewing the relevant literature of CNKI，Wanfang，PubMed and other databases，in order to provide an important reference and theoretical basis for Banxia Xiexin Decoction in the prevention and treatment of CAG.
The theory of“water entering meridians，and blood being formed”originates from ZHANG Zhongjing's Treatise on Febrile Diseases-Pulse Diagnosis and is a comprehensive summary of the absorption，circulation and transformation of water in traditional Chinese medicine. The process of water entering the meridians and transforming into blood marks the beginning of its circulation and function within the body. During this process，the spleen，meridians and kidney play vital roles. When water fails to enter the meridians to transform into blood，it forms pathological products such as phlegm，retained fluid and dampness. These pathological products，when accumulated in the lower limbs，obstruct normal blood flow and lead to the mutual obstruction of water and blood，resulting in edema. This mechanism bears a resemblance to the pathogenesis of chronic venous edema of the lower limbs，where venous hypertension in the lower limbs causes tissue fluid reflux obstruction and changes in vascular permeability. Based on the theory of“water entering meridians，and blood being formed”，Professor CAO Jianchun，the chief physician at the Peripheral Vascular Department of Dongfang Hospital has proposed that the treatment of chronic venous edema of the lower limbs should start from the physiological and pathological mechanisms of water entering the meridian circulation，adopt the method of promoting blood circulation and urination，reuse Huangqi（Astragali Radix）to invigorate spleen，Zelan（Lycopi Herba）to promote blood circulation，Zexie（Alismatis Rhizoma）to promote urination，and supplement with Guizhi（Cinnamomi Ramulus）and other spleen-invigorating，urination-promoting and blood-activating drugs，to achieve the process of invigorating spleen and tonifying Qi，regulating the absorption of water into the meridians to treat the root cause，promoting blood circulation and removing blood stasis，improving the process of water returning to the meridians and transforming blood between the skin and flesh，promoting urination and reducing swelling，and promoting the excretion of water through the meridians and kidney to the human body for external treatment.
To investigate the effects of Tianxiong Dingxuan Decoction（天芎定眩汤）combined with citicoline on TCM syndrome and cerebral hemodynamics in elderly patients with post-circulation ischemic vertigo of phlegm and blood stasis obstructing orifices type.
A total of 96 elderly patients with post-circulation ischemic vertigo of phlegm and blood stasis obstructing orifices type from March 2021 to December 2023 in the Department of Encephalopathy of Zhanjiang Second Hospital of Traditional Chinese Medicine were selected as the study objects. According to different treatment plans，they were divided into study group and control group，with 48 cases in each group. The control group received conventional Western medicine treatment such as citicoline. The study group was treated with Tianxiong Dingxuan Decoction on the basis of the control group. Both groups were treated for 2 weeks. The changes of TCM syndrome scores，Dizziness Handicap Inventory（DHI）score，cerebral hemodynamic indexes（flow velocity of basilar artery，left vertebral artery and right vertebral artery），plasma viscosity，whole blood viscosity and fibrinogen level were compared before and after treatment between the two groups. The efficacy of the two groups after 2 weeks and the adverse reactions during treatment were compared.
After treatment，the scores of dizziness，nausea and vomiting，fatigue，tinnitus and deafness and clouded heavy head，DHI score，the levels of plasma，whole blood viscosity and fibrinogen were significantly decreased in both groups，and the results of the study group were significantly lower than those of the control group（P<0.05）. After treatment，the cerebral blood flow velocity in the study group was significantly higher than that in the control group（P<0.05）. The total effective rate of the study group was significantly higher than that of the control group（P<0.05）. There was no significant difference in adverse reactions between the two groups during treatment（P>0.05）.
Tianxiong Dingxuan Decoction combined with citicylcholine can significantly improve the TCM symptoms and cerebral hemodynamic indexes of elderly patients with post-circulation ischemic vertigo of phlegm and blood stasis obstructing orifices type. It has accurate efficacy and high safety，and is worthy of promoting in the treatment of integrated traditional Chinese and Western medicine.
To investigate the clinical efficacy and mechanism of Peiyuan Huoxue Shoutai Decoction（培元活血寿胎方）combined with enoxaparine in the treatment of recurrent spontaneous abortion associated with antiphospholipid syndrome.
Ninety-six patients with recurrent spontaneous abortion associated with antiphospholipid syndrome admitted to the hospital between January 2021 and June 2023 who fulfilled the inclusion and exclusion criteria were enrolled，and classified into two groups using numerical parity method，each with 48 cases. The control group was given enoxaparin，and the observation group was given Peiyuan Huoxue Shoutai Decoction combined with enoxaparine. Then the clinical efficacy，TCM syndrome scores（vaginal bleeding，soreness and weakness of waist and knees，pale tongue，dizziness and tinnitus，frequent urination at night），coagulation function parameter，negative rate of antiphospholipid antibodies，pregnancy outcomes and adverse reaction rate were compared between two groups.
The observation group reported a higher clinical efficacy rate compared to the control group（91.67% vs 77.08%，P<0.05）. The improvement in TCM syndrome scores including vaginal bleeding，soreness and weakness of waist and knees，pale tongue，dizziness and tinnitus，frequent urination at night was more notable in the observation group than that in the control group after treatment（P<0.05）. The reduction in D-dimer（D-D）and fibrinogen（FIB）and the increase in prothrombin time（PT）and activated partial thromboplastin time（APTT）were more remarkable in the observation group than those in the control group（P<0.05）. The total conversion rate of antiphospholipid antibodies including anticardiolipin antibody，lupus anticoagulant and anti-β2 glycoprotein-I antibody was 91.67% in the observation group，which was higher than that（75.00%）in the control group（P<0.05）. Tocolytic success rate was 83.33% in the observation group，which was higher than that（58.33%）in the control group（P<0.05）. The total adverse reaction rate during treatment yielded no statistical difference between two groups［4.17%（2/48）vs 10.42%（5/48）］（P>0.05）.
The application of Peiyuan Huoxue Shoutai Decoction combined with enoxaparine for patients with recurrent spontaneous abortion associated with antiphospholipid syndrome can effectively ameliorate the clinical symptoms，improve the coagulation function，enhance the conversion rate of antiphospholipid antibody and increase the tocolytic success rate，which has a good safety profile and is worthy of recommendation.
To observe the effects of Modified Xiayuxue Decoction（下瘀血汤加减）assisted with percutaneous hepatic arterial chemoembolization（TACE）on levels of serum matrix metalloproteinase 1（MMP-1）and matrix metalloproteinase9（MMP-9）and liver function in patients with primary liver cancer.
This study selected 74 primary hepatocellular carcinoma patients who were treated in the oncology department from January 2019 to December 2022. All patients were diagnosed with liver heat and blood stasis syndrome. They were randomly divided into a control group（37 patients completed the study）and an observation group（37 patients completed the study）using simple randomization. All 74 patients received TACE treatment. The control group was given Compound Glycyrrhizin Tablets after surgery，while the observation group was given Modified Xiayuxue Decoction after surgery，continuously administered for 12 weeks. The therapeutic effects，overall Karnofsky performance status and safety of the two groups were statistically analyzed. The levels of MMP-1，MMP-9，alpha fetoprotein（AFP），AFP-L3，tumor tissue infiltration，liver function，serum albumin（ALB）and T lymphocyte subgroups were detected to evaluate the traditional Chinese medicine syndrome scores of the two groups.
The effective rate of the observation group was 64.86%（24/37），which was higher than that（40.54%，15/37）of the control group with statistical difference（P<0.05）. There was no statistical difference（P>0.05）in MMP-1，MMP-9，AFP，liver function，AFP-L3 or ALB between the two groups before treatment. After 12 weeks of treatment，the levels of MMP-1，MMP-9，liver function，AFP and AFP-L3 were lower than those before treatment and ALB was higher than that before treatment in both groups（P<0.05）. The levels of MMP-1，MMP-9，AFP and AFP-L3 were lower and ALB was higher in the observation group than those in the control group after 12 weeks of treatment（P<0.05）. There was no statistical difference（P>0.05）in tumor tissue infiltration between the two groups before treatment. After 12 weeks of treatment，the values of blood volume（BV）and blood flow（BF）were lower than those before treatment and mean transit time（MTT）was higher than that before treatment in both groups（P<0.05）. The tumor tissue infiltration in the observation group was compared with that in the control group after 12 weeks of treatment，with no statistical difference（P>0.05）. There was no statistical difference（P>0.05）in T lymphocyte subgroups between the two groups before treatment. After 12 weeks of treatment in the observation group，the levels of CD3＋，CD4＋and CD4＋/CD8＋were higher than those before treatment and CD8＋was lower than that before treatment（P<0.05）. There was no statistical difference（P>0.05）in T lymphocyte subgroups before and after treatment in the control group. The levels of CD3＋，CD4＋and CD4＋/CD8＋were higher and CD8＋was lower in the observation group than those in the control group after 12 weeks of treatment（P<0.05）. The overall Karnofsky score was higher in the observation group than that in the control group，and the total incidence of adverse reactions was not statistically different（P>0.05）when compared with those in the control group. There was no statistical difference（P>0.05）in the scores of 3 main symptoms and 7 collateral symptoms between the two groups before treatment. The scores of 3 main symptoms and 7 collateral symptoms were lower after 12 weeks of treatment than those before treatment in both groups（P<0.05）. The scores of 3 main symptoms and 7 collateral symptoms after 12 weeks of treatment were lower in the observation group than those in the control group（P<0.05）.
In the treatment of liver blood stasis syndrome with TACE，Modified Xiayuxue Decoction can improve liver function，do not affect blood perfusion of tumor tissue，inhibit the levels of MMP-1，MMP-9，AFPand AFP-L3，and alleviate symptoms.
To observe the clinical efficacy and safety of the treatment of insomnia of heart-kidney Yang deficiency type with Fuzi Decoction（附子汤）combined with Guizhi Gancao Longgu Muli Decoction（桂枝甘草龙骨牡蛎汤）.
A total of 114 patients with insomnia due to deficiency of heart and kidney yang were enrolled from January 2022 to December 2023. Simple randomization was performed using a random number table. The Western medicine group（n＝57）received zopiclone treatment，while the pattern differentiation group（n＝57）received Fuzi Tang combined with Guizhi Gancao Longgu Muli Tang. Both groups underwent continuous treatment for 4 weeks. The following parameters were assessed：Hamilton Anxiety Scale（HAMA）scores，5-hydroxytryptamine（5-HT），glutamate，gamma-aminobutyric acid（GABA），melatonin，Pittsburgh Sleep Quality Index（PSQI）scores，sleep architecture，TCM syndrome scores，and Hamilton Depression Scale（HAMD）scores. Efficacy and safety were statistically analyzed between groups.
Following treatment，both groups exhibited reduced HAMD scores，PSQI scores，TCM syndrome scores，and HAMA scores compared to pre-treatment levels，with the patterndifferentiation group showing lower scores than the Western medicine group（P<0.05）. The pattern-differentiation group achieved a total effective rate of 92.98% （53/57 cases），surpassing the Western medicine group's rate of 78.95% （45/57 cases）（P<0.05）. Post-treatment levels of serotonin and glutamate decreased in both groups compared to pre-treatment levels，with the pattern-differentiation group showing a greater reduction than the Western medicine group（P<0.05）. Post-treatment levels of gamma-aminobutyric acid（GABA）and melatonin increased in both groups compared to pre-treatment levels，with the pattern-differentiation group showing a greater increase than the Western medicine group（P<0.05）. After treatment，both groups showed reduced sleep latency（SOL）and wake-up time（WASO）compared to pre-treatment levels，with the pattern-differentiation group exhibiting a greater reduction than the Western medicine group（P<0.05）. Following treatment，both groups exhibited increased total sleep time（TST）and sleep efficiency（SE）compared to pre-treatment levels，with the pattern-differentiation group showing greater improvement than the Western medicine group（P<0.05）. The overall adverse reaction rate in the pattern-differentiation group was 5.26% （3/57 cases），compared to 7.02% （4/57 cases）in the Western medicine group，with no statistically significant difference（P>0.05）.
The treatment of heart-kidney Yang deficiency type of insomnia by Fuzi Decoction combined with Guizhi Gancao Longgu Muli Decoction can regulate the neurotransmitters，improve the sleep structure and enhance the clinical efficacy.
To investigate the effects of Buzhong Yiqi Decoction（补中益气汤）combined with Huaier Granules（槐耳颗粒）on swallowing function as well as inflammatory factors after esophageal cancer surgery.
Eighty-four patients with esophageal cancer who met the inclusion criteria were selected and divided into the reference group and treatment group according to the ratio of 1∶1 by random number table method. Both the reference and treatment groups received general treatment. The reference group was treated with traditional Chinese medicine placebo in combination with conventional western medicine. The treatment group was treated with Buzhong Yiqi Decoction combined with Huaier Granules on the basis of western medicine treatment for 2 weeks. The changes of TCM syndrome points，adverse reactions，swallowing function，the levels of C-reactive protein（CRP），interleukin-6（IL-6），interleukin-1β（IL-1β），squamous cell carcinoma antigen（SCC-Ag），carbohydrate antigen 19-9（CA19-9），carcinoembryonic antigen（CEA）and quality of life scores of the two groups were observed before and after treatment.
There was no significant difference between the two groups in all the observational indicators before treatment（P>0.05）. After receiving the treatment，all the indexes of the reference group and the treatment group improved（P<0.05）. And in the treatment group，the improvement of the TCM syndrome points，swallowing function，inflammatory factor levels and tumor marker level were more obvious，and the quality of life was significantly improved（P<0.05）.
The combination of Buzhong Yiqi Decoction and Huaier Granules can effectively improve the swallowing function and the effect of inflammatory factors after esophageal cancer surgery. Moreover，it can reduce the postoperative stress reaction of patients with esophageal cancer，decrease the levels of tumor markers and improve the quality of life of patients.
To explore the effects of Xionggui Zhitong Decoction（芎归止痛汤）combined with carbamazepine on pain，sleep，inflammatory factors and oxidative stress in patients with primary trigeminal neuralgia（PTN）.
A hundred cases of PTN patients were randomly divided into observation group and study group（50 cases in each group）. The two group were treated with carbamazepine，and the study group was treated with Xionggui Zhitong Decoction on the basis of carbamazepine. The changes of pain，sleep，inflammatory factors and oxidative stress before and after treatment were observed.
The present pain intensity score（PPI），pain rating index score（PRI）and visual analogue scale（VAS）score，Pittsburgh Sleep Quality Index（PSQI）score，the levels of serum substance P（SP），vasoactive intestinal peptide（VIP），tumor necrosis factor-α（TNF-α），interleukin-6（IL-6），interleukin-1β（IL-1β）and malondialdehyde（MDA）of the two groups after treatment were lower than those before treatment（P<0.05），the levels ofβ-endorphin（β-EP），superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）were higher than those before treatment（P<0.05）. After treatment，the scores of PPI，PRI，VASand PSQI scores，the levels of serum SP，VIP，TNF-α，IL-6，IL-1βand MDA in the study group were lower than those in the control group（P<0.05），and the levels ofβ-EP，SOD and GSH-Px in the study group were higher than those in the control group（P<0.05）. The therapeutic effect of the study group was better than that of the control group（P<0.05）.
Xionggui Zhitong Decoction combined with carbamazepine in the treatment of primary trigeminal neuralgia can help to improve the pain and sleep status of patients，reduce the levels of inflammatory factors and oxidative stress，and improve the clinical therapeutic effect.
To study the clinical effect of Anshen Jiaotai Yizhi Decoction（安神交泰益智汤）in the treatment of Parkinson's disease with sleep disorders and its effect on the levels of homocysteine（Hcy）and interleukin-1β（IL-1β）in patients.
110 patients with Parkinson's disease and sleep disorders were selected and divided into two groups，55 patients in the control group were treated with western medicine，and 55 patients in the observation group were treated with western medicine combined with Anshen Jiaotai Yizhi Decoction. After 2 weeks of treatment，the clinical efficacy，sleep quality，sleep structure index，related factor levels and adverse reactions were compared.
The total effective rate of the observation group（96.36%，53/55）was higher than that（83.64%，46/55）of the control group（P<0.05）. The Epworth sleepiness scale（ESS）scores after 2 weeks of treatment of the observation group was（7.92±1.83）points，and that of the control group was（9.14±2.11）points（P<0.05）. The sleep efficiency of the two groups increased，the sleep latency shortened，the number of awakenings decreased，the apnea hypopnea index（AHI）decreased，and the minimum oxygen saturation increased. The indexes of the observation group were significantly better than those of the control group［（78.89±6.20）%，（23.10±4.08）min，（1.97±0.56）times，（9.08±1.03），（89.15±5.80）% vs（73.39±6.14）%，（27.82±5.26）min，（2.75±0.67）times，（11.71±1.34），（86.30±5.52）%］（P<0.05）. The levels of serum Hcy and IL-1βin the two groups decreased，and the levels of the observation group were significantly lower than those in the control group［（1.01±0.06）mg/L，（8.90±1.85）pg/mL vs（1.20±0.06）mg/L，（10.13±1.91）pg/mL］（P<0.05）. The incidence of adverse reactions of the two groups had no significance（14.55%，8/55 vs 10.91%，6/55）（P>0.05）.
Anshen Jiaotai Yizhi Decoction has a better clinical effect in the treatment of Parkinson's disease with sleep disorders. It can significantly improve sleep status and regulate the neuroimmune inflammatory response in the brain. It is a safe and effective treatment plan and is worthy of application.
To explore the clinical effect of Modified Juanbi Decoction（蠲痹汤加减）combined with thunder-fire moxibustion at Dazhui（DU14）in the treatment of cervical spondylosis.
A total of 120 patients with cervical spondylosis（cold-dampness blocking collateral type）admitted from September 2022 to September 2023 were included in the study. The patients were divided into the conventional group and the traditional Chinese medicine group by random number table method，with 60 cases in each group. The conventional group was treated with Celecoxib Capsule，while the traditional Chinese medicine group was treated with Celecoxib Capsule and Modified Juanbi Decoction combined with thunder-fire moxibustion at Dazhui（DU14）. During the study，4 cases fell off in the conventional group and 3 cases fell off in the traditional Chinese medicine group. The data of each group，including clinical treatment efficacy，the traditional Chinese medicine syndrome scores（neck and shoulder pain and numbness，chest tightness，headache，nausea，upper limb heaviness，etc.）before and after treatment，serum the levels of transforming growth factor-β1（TGF-β1），interleukin-1β（IL-1β）and tumor necrosis factor alpha（TNF-α），the changes of cervical physiological curvature and pain scale（visual analog scale，VAS）scores，the cervical spondylosis（CASCS）scores and neck disability index（NDI）scores and adverse reactions were observed and compared.
The total effective rate of the traditional Chinese medicine group（96.49%，55/57）was higher than that of the conventional group（85.71%，48/56）（P<0.05）. After treatment，the TCM syndrome scores（neck and shoulder pain and numbness，chest tightness，headache，nausea，upper limb heaviness，etc.），the levels of TGF-β1，IL-1βand TNF-αand VASscores were all decreased in both groups，physiological curvature indexes of cervical vertebra were increased，CASCSscores and NDI scores were improved. The above indexes in the traditional Chinese medicine group were lower than those in the conventional group，and the physiological curvature of cervical spine was higher than that in the conventional group，CASCSscores and NDI scores were higher than those in the conventional group（P<0.05）. The adverse reaction rate of the traditional Chinese medicine group（1.75%，1/57）was lower than that of the conventional group（12.50%，7/56）（P<0.05）.
Modified Juanbi Decoction combined with thunder-fire moxibustion at Dazhui（DU14）in the treatment of cervical spondylosis（cold-dampness blocking collateral type）has good clinical effect，with improved symptoms and better cervical function recovery，and the treatment is safe and reliable.
To explore the clinical efficacy of Sangju Qingjie Decoction（桑菊清解汤）combined with symptomatic and supportive therapy including anti-infection，antipyretic therapy，fluid resuscitation and aerosol inhalation in children with lobar pneumonia.
A total of 198 children with lobar pneumonia were classified into two groups，each with 99 cases. The control group was given symptomatic supportive treatment such as anti-infection，antipyretic therapy，fluid replenishment and aerosol inhalation，while the observation group was given Sangju Qingjie Decoction on the basis of the control group. The levels of high-sensitivity C-reactive protein（hs-CRP），white blood cells（WBC），erythrocyte sedimentation rate（ESR），interleukin-6（IL-6），peak expiratory flow（PEF），forced respiratory volume in 1 second（FEV1）and FEV1 versus forced vital capacity（FEV1/FVC）were compared between the two groups before and after treatment. Meantime，the time of fever reduction，the disappearance time of cough，sputum and wheezing，and hospital stay length，clinical efficacy and adverse reaction rate were also observed in both groups.
The total efficacy rate in the observation group（97.98%，97/99）was higher than that in the control group（84.85%，84/99）with statistical difference（P<0.05）. The TCM syndrome scores including cough，wheezing，expectoration and wheezing yielded no statistical difference between two groups before treatment（P>0.05），and the scores were descended in two groups after treatment（P<0.05）. There were statistical difference before and after treatment in two groups（P<0.05）. After treatment，TCM syndromes score of the observation group was lower than that of the control group（P<0.05）. The observation group had descended time to fever reduction，cough disappearance，sputum disappearance，wheezing disappearance and hospital stay length compared with the control group（P<0.05）. The levels of IL-6，WBC，hs-CRPand ESR of two groups before treatment showed no statistical difference（P>0.05），and had statistical difference after treatment（P<0.05）. After treatment，the levels of IL-6，WBC，hs-CRPand ESR were lower in the observation group than those of the control group（P<0.05）. No statistical difference was found in FEV1，PEF or FEV1/FVC before treatment（P>0.05），while the post-treatment FEV1，PEF and FEV1/FVCdenoted statistical difference with those before treatment（P<0.05）. The three pulmonary function indicators were higher in the observation group than those of the control（P<0.05）. After treatment，the total adverse reaction rate was 5.05%（5/99）in the observation group，which was slightly lower than that of the control group（7.07%，7/99）（P>0.05）.
The application of Sangju Qingjie Decoction combined with symptomatic and supportive therapy including anti-infection，antipyretic therapy，fluid resuscitation and aerosol inhalation in children with lobar pneumonia can remarkably improve the clinical outcome in children by shortening the duration of treatment，cutting down the rate of adverse reaction，and improving lung function and the serum levels of inflammatory factors.
To study the changes in endothelin（ET），serum neuropeptide Y（NPY）and substance P（SP）in patients treated with Modified Jichuan Decoction（济川煎加味）combined with abdominal acupuncture for slow-transmitting constipation with Yang-deficiency of spleen and kidney.
Ninety-four patients with slow-transmission constipation（Yang-deficiency of spleen and kidney）were divided into two groups by using random number method. The two groups were given general treatment，such as regular life，balanced diet，appropriate participation in physical exercise，drinking more water，etc. The Mosapride group was treated with Mosapride，and the Jichuan Decoction group was given Modified Jichuan Decoction combined with abdominal acupuncture on the basis of the Mosapride group. The time of first defecation，constipation symptom scores（including difficulty in defecation，frequency，time and faecal character，feeling of falling and incompletion，abdominal distension），the points of the Patient's Assessment of Quality of Life Scale（PAC-QOL）for constipated patients and the changes of ET，nitric oxide（NO），NPY and SP were compared between the two groups.
The overall time to first defecation in the Jichuan Decoction group was statistically better than that in the Mosapride group（P<0.05）. There was no statistical significance when comparing the scores of constipation symptoms such as difficulty in defecation，frequency，time and faecal characteristics，feeling of incompleteness and abdominal distension before treatment between the two groups（P>0.05）. The constipation symptom scores of Jichuan Decoction group and Mosapride group were lower after treatment than before treatment，and the constipation symptom scores of Jichuan Decoction group were lower than those of Mosapride group after treatment（P<0.05）. There was no statistical significance in the comparison of ET，NO，NPY or SP before treatment between the two groups（P>0.05）. The levels of ET，NO and NPY decreased and the level of SP increased after treatment in the Jichuan Decoction group and the Mosapride group compared with those before treatment（P<0.05）. The level of SP was higher in the Jichuan Decoction group than that in the Mosapride group，and the levels of ET，NO and NPY were lower than those in the Mosapride group after treatment（P<0.05）. There was no statistical significance in the comparison of PAC-QOL score before treatment between the two groups（P>0.05）. The PAC-QOL scores of Jichuan Decoction group and Mosapride group were lower after treatment than those before treatment，and the PAC-QOL scores of Jichuan Decoction group were lower than those of Mosapride group after treatment（P<0.05）.
Modified Jichuan Decoction combined with abdominal acupuncture can regulate the expression levels of ET，NO，NPY and SPin patients with slow-transmitting constipation with Yang-deficiency of spleen and kidney，improve the symptoms of constipation，promote the initial defecation and improve the quality of life.
To study the effect of Qingfengteng Decoction（青风藤汤）on the changes of CXCchemokine ligand 13（CXCL13）and macrophage inflammatory protein 1α（MIP-1α）in patients with rheumatoid arthritis.
Seventy-eight patients with rheumatoid arthritis were selected and divided into two groups by using the random number method. The methotrexate group was given methotrexate tablets for treatment，and the Qingfengteng Decoction group added Qingfengteng Decoction for adjuvant treatment to analyse the effect of the two groups after treatment.
The comparison of morning stiffness time，twohanded grip strength，number of joint pressure pain and 20 m walking time in the two groups showed no statistical difference（P>0.05）. Comparing the two groups before and after treatment，morning stiffness time，the number of joint pressure pain and 20 m walking time decreased in both groups，and the morning stiffness time，the number of joint pressure pain and 20 m walking time were lower in the Qingfengteng Decoction group than those in the methotrexate group after treatment（P<0.05）. Hands grip strength was elevated in both groups，and hands grip strength was higher in the Qingfengteng Decoction group than that in the methotrexate group after treatment（P<0.05）. The comparison of CXCL13，MIP-1α，ESR and interleukin between the two groups showed no statistical difference（P>0.05）. Comparing the two groups before and after treatment，the levels of interleukin-21（IL-21），CXCL13，blood sedimentation rate（ESR），interleukin-27（IL-27），MIP-1αand interleukin-32（IL-32）were reduced in the two groups，and in the Qingfengteng Decoction group，they were all lower than those of the methotrexate group after treatment（P<0.05）. There was no statistically significant difference in disease activity score 28（DAS28）between the two groups（P>0.05）. Comparing the two groups before and after treatment，the DAS28 scores of both groups were reduced，and the score of the Qingfengteng Decoction group was lower than that of the methotrexate group after treatment（P<0.05）.
The adjuvant treatment of rheumatoid arthritis can inhibit the expression levels of CXCL13，MIP1αand ESR，reduce the inflammatory response and disease activity and relieve the clinical symptoms.
Banxia（Pinelliae Rhizoma）was first published in Shennong Bencao Jing，with a long history of medication，outstanding clinical application and a wide range of compatibility. The seven relations of medicinal compatibility include single，mutual reinforcement，mutual assistance，mutual restraint，mutual suppression，mutual inhibition and antagonism. The theory of seven relations of medicinal compatibility in traditional Chinese medicine refers to the use of drugs together to achieve synergistic or attenuating effects. Based on the literature of CNKI and combined with the characteristics of clinical use，this paper sorted out and excavated the commonly used drug pairs of Banxia（Pinelliae Rhizoma），and it is found that Banxia（Pinelliae Rhizoma）is often used with Houpo（Magnoliae Officinalis Cortex），Huanglian（Coptidis Rhizoma），Maidong（Ophiopogonis Radix），Renshen（Ginseng Radix et Rhizoma），Baizhu（Atractylodis Macrocephalae Rhizoma），Chenpi（Citri Reticulatae Pericarpium）and Xiakucao（Prunellae Spica）with the relations of mutual reinforcement and mutual assistance，used with Shengjiang（Zingiberis Rhizoma Recens）and Gancao（Glycyrrhizae Radix et Rhizoma）with the relations of mutual restraint and mutual suppression，and used with Fuzi（Aconiti Lateralis Radix Praeparata）and Chuanwu（Aconiti Radix）with the relations of antagonism. Through the combination of traditional Chinese medicine theory and modern pharmacology，it is concluded that Banxia（Pinelliae Rhizoma）has many functions such as gastrointestinal protection，nerve regulation，anti-coagulation，anti-depression，anti-inflammation and anti-tumor，which verifies the rationality of the theory of seven relations of medicinal compatibility in traditional Chinese medicine and provides ideas for the use law and clinical treatment of Banxia（Pinelliae Rhizoma）and its drug pairs.
To compare the effects of fresh cut pieces and traditional pieces of Huangqin（Scutellariae Radix），providing scientific basis for quality control of Huangqin（Scutellariae Radix）pieces with different processing method.
Ultraviolet spectrophotometry（UV）and ultra-high performance liquid chromatography（UPLC）and other methods were used to determine the contents of 6 index components（baicalin，wogonoside，baicalein，wogonin，total flavonoids，alcohol-soluble extract）in 24 batches fresh cut and traditional pieces of Huangqin（Scutellariae Radix）from different producing areas as samples. The bivariate correlation analysis of Huangqin（Scutellariae Radix）pieces was conducted by SPSS22.0 software in terms of the content of alcohol-soluble extract，total flavonoids，baicalin，wogonoside，baicalein and wogonin.
The content of alcohol-soluble extract in 24 batches of Huangqin（Scutellariae Radix）fresh cut pieces were（46.44±0.06）%～（55.26±0.21）%，the contents of total flavonoids，baicalin，wogonoside，baicalein and wogonin were（215.90±0.14）～（288.87±0.21）mg/g，（150.10±0.34）～（199.40±0.19）mg/g，（27.72±0.04）～（39.85±0.15）mg/g，（2.45±0.00）～（8.63±0.01）mg/g，（0.56±0.00）～（2.43±0.01）mg/g，respectively. The contents of alcohol-soluble extract in 24 batches of Huangqin（Scutellariae Radix）traditional pieces were（44.69±0.04）%～（54.24±0.07）%，the contents of total flavonoids，baicalin，wogonoside，baicalein and wogonin were（214.16±0.14）～（286.08±0.00）mg/g，（155.82±0.08）～（197.62±0.18）mg/g，（28.16±0.02）～（38.24±0.00）mg/g，（2.11±0.02）～（5.84±0.03）mg/g，（0.43±0.00）～（1.24±0.00）mg/g，respectively. Compared with those in the traditional cutting pieces，the contents of baicalein and wogonin were higher in fresh cutting pieces，whereas that of baicalin was lower in the fresh cutting pieces. Comprehensive evaluation of Huangqin（Scutellariae Radix）pieces were positively correlated with the contents of total flavonoids with the baicalin and wogonoside，the baicalin with wogonoside，the baicalein with wogonin（<0.01）.
The processing technology of fresh cutting is feasible in the production of Huangqin（Scutellariae Radix）pieces.
To investigate the clinical effect of Jianpi Shugan Tongru Prescription（健脾疏肝通乳方）combined with low frequency pulse electrical stimulation in patients with postpartum lactation deficiency（syndrome of weakness of Qi and blood）.
A total of 96 patients with postpartum lactation deficiency（syndrome of weakness of Qi and blood）admitted from January 2022 to December 2023 were included in this grouping study. The grouping method was random number table method，and they were divided into control group and observation group according to different treatment methods. The patients in the control group（48 cases）were given low-frequency pulse electrical stimulation treatment. Those in the observation group（48 cases）were given low-frequency pulse electrical stimulation and Jianpi Shugan Tongru Prescription，and the data of patients in each group was compared，including the clinical efficacy，the changes in TCM symptom scores，the levels of estradiol（E2）and prolactin（PRL）before and after treatment，the changes in breast filling degree，lactation volume and milk quality score，breastfeeding status，treatment adverse reactions and treatment satisfaction rate.
Compared with those in the control group，the total effective rate and satisfaction rate were higher in the observation group（P<0.05）. The rate of exclusive breastfeeding in the observation group was higher than that in the control group（P<0.05）. There was 1 case of bitter mouth in the observation group and no adverse reaction in the control group（P>0.05）. The scores of TCM syndrome，the levels of E2 and PRL，breast filling degree，lactation volume and milk quality scores of both groups were not significant before treatment（P>0.05）. After treatment，all indexes of both groups were improved，and all indexes of the observation group were better than those of the control group（P<0.05）.
The combination treatment of Jianpi Shugan Tonglu Prescription and low-frequency pulse electrical stimulation for patients with postpartum lactation deficiency（syndrome of weakness of Qi and blood）has a more significant effect. After treatment，the symptoms of patients are improved. They can better secrete milk，improve the rate of exclusive breastfeeding，meet the needs of newborns and have fewer adverse reactions and higher safety，and the patients are more satisfied and recognized.
Dahuang（Rhei Radix et Rhizoma）is a common medicinal material in ancient and modern times. It has anti-inflammatory，antibacterial，antioxidant，cardiac protection and other pharmacological effects，and can reduce the resistance of some cell lines to antibacterial drugs. The drug pairs are widely used in clinical practice. In this paper，the modern pharmacological research and related network pharmacology of drug pairs based on Dahuang（Rhei Radix et Rhizoma）-Taoren（Persicae Semen），Dahuang（Rhei Radix et Rhizoma）-Huanglian（Coptidis Rhizoma），Dahuang（Rhei Radix et Rhizoma）-Chishao（Paeoniae Radix Rubra），Dahuang（Rhei Radix et Rhizoma）-Huangqi（Astragali Radix），Dahuang（Rhei Radix et Rhizoma）-Chaihu（Bupleuri Radix），Dahuang（Rhei Radix et Rhizoma）-Gancao（Glycyrrhizae Radix Et Rhizoma），Dahuang（Rhei Radix et Rhizoma）-Jixuecao（Centellae Herba），Dahuang（Rhei Radix et Rhizoma）-Huangqin（Scutellariae Radix）in CNKI（China Knowledge Network）data in recent years were sorted out. This paper reviewed the research status and progress of Dahuang（Rhei Radix et Rhizoma）and its drug pairs，aiming to reveal the application rules and compatibility theory of the drug pairs and its future development direction in clinical application，so as to provide new ideas and basis for related research.
Chikungunya hemorrhagic fever（CHIK）is an acute infectious disease caused by the Chikungunya virus and transmitted by Aedes mosquitoes，primarily characterized by fever，rash and joint pain. Existing literature indicates that Yunnan and Fujian are regions with high incidence rates. The current outbreak in Shunde presents epidemiological and clinical features distinct from previous occurrences，reflecting the endemic characteristics commonly associated with diseases in the Lingnan region. Since the onset of the epidemic，the Shunde District Health Bureau and the Center for Disease Control and Prevention have jointly established an expert group dedicated to the diagnosis and treatment of CHIK using Traditional Chinese Medicine（TCM）. This expert group has conducted comprehensive analyses of syndrome elements during prevention，control and treatment processes at Lecong Hospital，Chencun Hospital and Beijiao Hospital，thereby accumulating substantial clinical evidence and practical experience. To standardize definitions，TCM disease nomenclature，syndrome differentiation，treatment protocols and efficacy evaluation criteria for this CHIK outbreak，and to enhance clinical management while improving patient prognosis and quality of life，the Chikungunya hemorrhagic fever TCM Treatment Expert Group has formulated a unified clinical treatment protocol addressing this critical issue. Drawing upon relevant domestic guidelines and integrating the actual clinical situation of CHIK cases in Shunde of Guangdong Province，the protocol has undergone revisions through the collaborative efforts of experts and now serves as a guiding document for the TCM-based diagnosis and treatment of CHIK in the Lingnan region. This treatment protocol is intended to assist physicians engaged in TCM emergency care in making appropriate clinical assessments and treatment decisions for patients with this acute infectious disease. It is designed solely as a reference for TCM practitioners in the diagnosis and treatment of CHIK and should be applied in conjunction with actual clinical circumstances.
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