Full
Outline
To explore whether Chonglou（Rhizoma paridis）has a reversal effect on multidrug resistance of leukemia CEM/VCR and its reversal mechanism.
CEM/VCR cells were treated with different concentrations of Chonglou（Rhizoma paridis）for 72 hours，and the mortality rate was calculated，and the appropriate concentration of Chonglou（Rhizoma paridis）reversal agent was selected. MTT colorimetric experiment was used to calculate the inhibitory rate and half inhibitory concentration（IC50）of low，medium and high concentrations（0.1，0.5 and 2.5μg/mL）of vincristine（VCR）acting on Chonglou（Rhizoma paridis）treated cells（hereinafter referred to as the treated group）and unweighted Chonglou（Rhizoma paridis）treated cells（hereinafter referred to as the untreated group）for 24，48 and 72 hours respectively，and to calculate the reversal index（RI）and reversal multiple（IC50）between the two groups. Flow cytometry（FCM）was used to detect the apoptosis rate after VCR was applied to the two groups of cells. RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of Bax，Bcl-2，Cyt-c，P-gp and MRP1 after VCR acted on the two groups of cells.
The concentration of Chonglou（Rhizoma paridis）reversal agent was 0.008μg/mL. The inhibition rate of VCR in the treatment group was significantly higher than that in the untreated group（P<0.05 or P<0.01）. The IC50 value of VCR in the treatment group after 24，48 and 72 hours was reduced from 17.32，13.85 and 6.21μg/mL to 15.37，2.25 and 0.62μg/mL，and the reversal multiples were 1.12 respectively. The results of flow cytometry showed that the apoptosis rate of VCR group increased gradually with the increase of drug concentration，and the apoptosis rate of treated group was higher than that of untreated group under the same conditions. The results of RT-qPCR and Western blot showed that the expressions of Bcl-2，P-gp，MRP1-1 and Bax，Cyt-c genes and proteins were down-regulated and up-regulated by VCR with different concentrations at the same time compared with those of the control group（P<0.05），and this change was more significant in the treatment group.
Chonglou（Rhizoma paridis）can reverse the multidrug resistance of CEM/VCR cells，which may be related to down-regulating the expressions of P-gp and MRP1，reducing the efflux of chemotherapeutic drugs and increasing the intracellular concentration of VCR，down-regulating the expression of Bcl-2 and up-regulating the expressions of Bax and Cyt-c，inducing cell apoptosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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