Full
Outline
To observe the effect of Shegan Mahuang Decoction（射干麻黄汤）on T lymphocyte subsets in upper and lower airways of airway hyperresponsiveness（AHR）mice，and analyze the biological basis of simultaneous treatment of nose and lung in AHR treatment.
Gammadelta（γδ）T cells were purified from beta T receptor knockout mice. AHR model mice sensitized with ovalbumin were randomly attributed into normal control group（Ctrl），AHRgroup（AHR），γδT cells adoptive immunization group（AIT），Shegan Mahuang Decoction group（SGMHD），Shegan Mahuang Decoction and adoptive immunization group（SMA）. AIT group was injectedγδT cells via tail vein daily，SGMHTgroup was given Shegan Mahuang Decoction daily by gavage，and SMA group was given dailyγδT cell tail vein injection combined with Shegan Mahuang Decoction by gavage for 7 days. T lymphocyte subsetsmarkers in nasal mucosa and lung of mice were detected，including interferon-γ（IFN-γ），interleukin-4（IL-4），retinoic acid related orphan receptorγt（RORγt），forkhead transcription factor P3（Fox P3）andγδTcell receptor（γδTCR）. The ratios of Th1，Th2，Th17，Treg andγδT cells，and the corresponding cytokines in peripheral blood were also measured.
The results showed that compared with those in the Ctrl group，the expressions of IFN-γ，IL-4，FoxP3，RORγt andγδTCR of nasal mucosa and lung were higher in AHR group，with higher proportions of Th2，Th17 andγδT cells，higher concentrations of IL-2，IL-4，IL-5 and IL-17，and lower concentrations of IL-10 and TGF-β. Compared with those in the AHR group，the expressions of IFN-γ，IL-4，FoxP3，RORγt andγδTCRof nasal mucosa were lower in the AIT，SGMHD and SMA groups，with lower levels of IL-4 andγδTCR in lung，higher ratio of Th1 and lower ratios of Th17 andγδT cells in peripheral blood. The levels of IL-2，IL-4，IL-5 and IL-17 of SGMHT and SMA groups were lower expressed，however the levels of IL-10 and TGF-βwere higher.
The study clarified the effect of Shegan Mahuang Decoction on AHR mice through simultaneous treatment of nose and lung. The underlying mechanism was related to the comprehensive regulation of T lymphocyte subsets. The study laid a foundation for revealing mechanisms of Shegan Mahuang Decoction in the treatment of AHR and promoting its clinical practice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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