Listeria monocytogenes (LM) is a foodborne pathogen that causes diseases with high rates of lethality, and its contamination is particularly problematic in ready-to-eat meat products. With the accelerated pace of modern life and the rapid development of society, the demand for ready-to-eat food has been rising, and the risk of contamination of ready-to-eat food by LM has increased. This not only poses a potential threat to the health and safety of consumers, but also has the potential to trigger significant economic losses. This paper comprehensively analyzed the outbreak cases of LM in ready-to-eat meat products, the relevant laws and regulations of ready-to-eat foods in specific countries, and the current biological control measures. Through the in-depth discussion of relevant elements, the aim was to comprehensively describe the control strategy of LM in ready-to-eat foods, and identified potential risks and challenges, so as to provide scientific guidance for the control of LM in the food industry.

Objective To established an analytical method for indirectdetermination of xylo-oligosaccharides in high gelatinization-bakedfoods by high performance liquid chromatography with refractive indexdetector. Methods After degreased with petroleum ether, the samples wereextracted with 20% ethanol solution, and macromolecular sugars such asdextrin and starch were precipitated with 68% ethanol solution. Thesugars in the extraction solution were hydrolyzed into monosaccharidesby diluted sulfuric acid, the solution before and after hydrolysis wasseparated by amide column, detected by refractive index detector, andquantified by external standard method. The product between thedifference of xylose before and after hydrolysis and the averageconversion coefficient of xylo-oligosaccharides and xylose was thexylo-oligosaccharides. Results When the xylose was ${0.25}\sim {5.00}\mathrm{{mg}}/\mathrm{{mL}}$ ,there was a good linear relationship between peak area and concentration $\left({r ={0.9998}}\right)$ . Therecovery rates ranged from 92.0% to 95.8%, the relative standarddeviations (RSDs) were ${1.8}\%\sim {4.0}\%$ in highgelatinization-baked foods. The limit of detection and limit ofquantization of xylo-oligosaccharides were ${0.03}\mathrm{\;g}/{100}\mathrm{\;g},{0.1}\mathrm{\;g}/{100}\mathrm{\;g}$ respectively. Conclusion The extraction method of low concentrationethanol solution is feasible and specific. The method avoided the impactof high gelatinization on the low extraction efficiency and measurementresults. It can determine xylo-oligosaccharides in highgelatinization-baked foods accurately and quickly.

Food contaminants pose a serious challenge to public health and the food industry, and there is an urgent need to develop more advanced detection techniques and updated prevention strategies. Although the traditional culture technology has played an important role in the detection of food contaminants, there are obvious limitations in the detection speed, sensitivity and specificity. With the increasing expansion of global food trade, the growing production scale of food enterprises, and the increasing attention of the public to food safety, food safety has become a hot topic around the world. Food contaminants are mainly divided into chemical and biological categories, including but not limited to pesticide and veterinary drug residues, heavy metal ions, abused food additives, foodborne pathogens and mycotoxins. In recent years, with the rapid development of cutting-edge technologies such as molecular biology technology, biosensors, nanotechnology and advanced imaging technology, these innovative methods are gradually overcoming the limitations of traditional detection methods. This paper discussed the principles, advantages and limitations of these innovative detection technologies in depth, and reviewed the latest research progress in the field of food detection. By comparing different detection methods, this paper aims to provide guidance for selecting suitable detection technology to achieve rapid and accurate detection and analysis of food contaminants.

Objective To evaluate the uncertainty in the determinationof thiamethoxam content in corn by high-performance liquidchromatography-tandem mass spectrometry. Methods According to GB/T20770-2008 Determination of 486 Pesticides and Related ChemicalsResidues in Grains-Liquid Chromatography-Tandem Mass Spectrometrymethod, by establishing a mathematical model, the sources of uncertaintywere analyzed and the effects of different sources were measured andcalculated. Results Forthe corn samples with a mass fraction of ${0.282}\mathrm{{mg}}/\mathrm{{kg}}$ ofthiamethoxam, the extended uncertainty was ${0.041}\mathrm{{mg}}/\mathrm{{kg}}\left({P={95}\%, k = 2}\right)$ . Conclusion The main source ofuncertainty affecting the test result is the determination of samplesolution.

Objective To improve and establish a molecular fluorescencespectrophotometry method for determining the content of vitamin ${\mathrm{B}}_{2}$ in animal derived foods.Methods Using pork belly, chicken, pig liver, and cow kidney as researchobjects, and referring to GB 5009.85-2016 National Standard of FoodSafety-Determination of Vitamin ${B}_{2}$ in Foods, further simplified theexperimental process, optimize experimental conditions, analyzed variousfactors that affect the results during the detection process, such as $\mathrm{{pH}}$ , light intensity, andthe effect of sodium hydrosulfite on fluorescence quenching, improvedthe accuracy of the method for determining vitamin ${\mathrm{B}}_{2}$ in animal derived foods.Results The maximum fluorescence intensity was determined at pH 6.5. Asthe light intensity increased and the light duration increased, thefluorescence intensity gradually decreased. After 5 hours, vitamin ${\mathrm{B}}_{2}$ basically decomposedcompletely. Vitamin ${\mathrm{B}}_{2}$ exhibited good linearitywithin the range of $0 \sim {10\mu}\mathrm{g}$ , with ${r}^{2}={0.9993}$ ; the limit of detection was ${0.004}\mathrm{{mg}}/{100}\mathrm{\;g}$ ,and the limit of quantification was ${0.02}\mathrm{{mg}}/{100}\mathrm{\;g}$ .The recovery rates for spiking ranged from ${90}\%\sim {100}\%$ . The relativestandard deviation of parallel sample determination results was lessthan 6%. Conclusion This method is accurate, simple in equipment, andcost-effective, and can meet the needs of grassroots laboratories forthe determination of vitamin ${\mathrm{B}}_{2}$ in animal derivedfoods.

Objective To evaluate of uncertainty in the determination of residual cyclopropamide in animal food by high-performance liquid chromatography. Methods The sample was repeatedly extracted by the mixture of Trichloroacetic acid and acetonitrile. The supernatant was degreased with n-hexane, and then concentrated by rotating evaporation in a water bath. The mixture of trichloroacetic acid and acetonitrile was redissolved, and then purified by a mixed cation exchange solid phase extraction column. After separation by an amino chromatographic column, acetonitrile water was used as the mobile phase for isocratic elution. The residue of cyclopromazine in animal food was quantitatively determined by diode array detector and external standard method. According to the JJF 1135-2005 Chemical Analysis Measurement Uncertainty Assessment and JJF 1059.1-2012 Measurement Uncertainty Assessment and Representation, the uncertaintygenerated during the entiretesting process includes weighing, standard, standard working curve formulation, standard curve fitting, sample measurement repeatability, instrument and standard recovery rate, and the uncertainty introduced by each factor is calculated, and finally theexpansionuncertainty is obtained. Results When the detection result of cyromazine in chicken was 19.8 µg/kg, with an expanded uncertainty of 3.2 µg/kg. Conclusion The main factors affecting uncertainty are the determination of standard, samples, the preparation of standard curves and the fitting of standard curves.

The issue of food quality and safety is a major issue related to the national economy and people's livelihood, and it is also a livelihood issue that the Chinese people are increasingly concerned about. Grain collection and storage enterprises shall establish a quality and safety traceability system to record and save food quality and safety information objectively, effectively and truly. This article aimed to understand the construction and operation status of the traceability system for grain storage enterprises through on-site research, document review, and conference communication. On the basis of drawing on the experience of traceability systems for grain storage enterprises at home and abroad, and in accordance with relevant laws, regulations, and standards such as the Food safety law of the People's Republic of China, the Opinions of the General Office of the State Council on Accelerating the Construction of Important Product Traceability Systems (State Council Office [2015] No.95), and the Opinions of the Food and Drug Administration on Promoting the Improvement of Traceability Systems for Food and Drug Manufacturers and Operators (Food and Drug Administration [2016] No.122), a quality and safety traceability system for grain storage enterprises was formulated, so as torealize the traceability of grain quality and safety in the forward direction, traceability in the reverse direction, and risk control. When quality and safety problems occur, products could be recalled, the causes could be investigated, and responsibilities could be pursued. Intended to provide reference suggestions for improving the quality and safety traceability system construction of grain storage enterprises, to ensure grain quality and safety, and to provide support for supervision.

Objective To compare the different extraction methods of soybean crude fatty acid value with the national standard method GB 5009.229-2016 National Standard forFood Safety-Determination of Acid Value in Food. Methods The room temperature extraction method, organic solvent oscillation method, and ultrasonic assisted method were used to detect the crude fatty acid value of soybeans, and compared with the Soxhlet extraction method required by the national standard method. Results The crude fatty acid value of soybeans measured by 3 kinds of extraction methods is basically consistent with the national standard method, and the extraction speed is significantly faster than the Soxhlet extraction method in the national standard. Among them, the ultrasonic assisted extraction method has the shortest extraction time, only requiring 20 minutes; as the sample size increases, the extraction efficiency of the room temperature extraction method improves more significantly. Conclusion Daily testing of grain and oil enterprises can shorten time, reduce costs, and improve efficiency by selecting suitable extraction methods based on sample batch size.

Laboratories in colleges and universities are important places for scientific practice, facing a large number of teachers and students, and involving the safety and stability of the campus environment. Aiming at the current situation of gaseous pollutant emission and management in teaching laboratories of colleges and universities at home and abroad, a comprehensive research has been carried out by means of web browsing, literature reading, field examination and questionnaire survey, etc. The research includes: types of gaseous pollutants in laboratories and methods of their disposal, the current situation and problems of gaseous pollutant emission in laboratories of domestic colleges and universities, the advantages of gaseous pollutant management in laboratories of foreign colleges and universities and the results of the analysis based on research Based on the results of the research, the study and the corresponding countermeasures are given, in order to provide reference for the emission and control of gaseous pollutants in teaching laboratories of universities in China.

Production safety is related to people's well-being and the overall situation of economic and social development. However, laboratory hazardous chemical safety-related incidents have occurred in recent years, which has sounded the alarm for laboratory safety management in colleges and universities. This paper focuses on the outstanding problems and weak links in the safety management of university laboratories and hazardous chemicals, and focuses on the work carried out by the university in the implementation of responsibilities, system construction, publicity and education, hazard source control, hidden danger investigation and rectification, emergency drill and information platform construction, so as to provide reference for the fine management of hazardous chemicals in university laboratories.

Under the new circumstances of rapid social and economic development in China, higher education is experiencing significant growth. The government is increasingly emphasizing the importance of laboratory safety in science and engineering universities. Given the significant roles of university laboratories in teaching and research, laboratory safety is characterized by diversification, complexity, and specialization. This paper analyzes the main challenges faced by safety management in engineering university laboratories and proposes improvement measures from four perspectives: strengthening and refining the laboratory safety management responsibility system, timely revision of safety-related documents, promoting safety awareness and education, intensifying laboratory safety inspections, establishing a laboratory safety management information system. These measures aim to enhance the overall level of laboratory safety management, providing solid support for the safe operation of laboratories and facilitating teaching and research activities.

Taking Jiangnan University as the practice subject, the paper studies the interdependence of the preparation, procurement, use and disposal stages in the full life cycle management system of large scientifiic research instruments and equipment.Taking this as the cornerstone, in view of the main problems existing in the equipment management. The improving measures are proposed including promoting information construction, making good budget demonstration, improving the performance appraisal, widening the channel of disposal, strengthening the team construction. The present study aimed at by means of actively exploring the fine full life cycle management of large scientifiic research instruments and equipment, improving management level of instruments and equipment in colleges and universities in the new era to better service for teaching and scientific research.

Aiming at the common problems existing in the scientific research laboratories of universities, such as the low level of information management, the unclear responsibility of laboratory management, the lack of equipment maintenance in the later stage and the low utilization ratio of instruments and equipment, this study proposes a"Flexible" laboratory management mechanism to solve the bottleneck problem of laboratory management. “Flexible management" is to optimize all kinds of existing soft and hard resources into a "Flexible" whole by establishing an information-based management platform, the invention solves the problems of low utilization rate of laboratory and instrument equipment, repeated purchase of the same kind of equipment, and difficulty in late-stage maintenance of equipment. Therefore, it is an inexorable trend of engineering laboratory construction to construct an efficient and extensible flexible mechanism.

In view of the current situation of laboratory management in local colleges and universities, in order to realize the reform of informatization of laboratory management system in local colleges and universities, it is proposed to build a lightweight laboratory information sharing platform. This paper analyzes the operation characteristics and existing problems of laboratories in local colleges and universities, and discusses the application value of information technology in laboratory management. On this basis, a low-cost and lightweight information sharing platform is constructed. Combined with the current difficulties of laboratory management in institutions, the platform is divided into four basic modules of instruments and equipment, reagents and consumables, users and information push for construction, in order to enhance the transparency and sharing efficiency of laboratory information and improve the laboratory management mode in local institutions.

As important bases for experimental teaching and medical research, the biosecurity of laboratories in medical universities cannot be ignored. In order to accurately grasp the research progress of biosecurity management in medical university laboratories in China, co-word cluster analysis was conducted on the 473 literatures related to biosecurity in medical laboratories published in the CNKI database from 2004 to 2023 using CiteSpace software. The research results showed that biosecurity, laboratory safety management, safety education, and medical testing are the four current research hotspots in this field, while medical research laboratories and safety education are potential directions for future research development.

For the experimental research of Xinjiang oilfield, all kinds of research work can not be separated from experiments. As an important place of scientific and technological research and development, the quality and level of scientific and technological research and development are directly affected by the management of the laboratory, therefore, it is of great significance to do a good job in the management of the laboratory. According to the actual situation of laboratory work, this paper summarizes the experience of laboratory management, and puts forward and implements the innovation mechanism of safety autonomous management in the course of experimental research from the aspects of creating safety concept culture and establishing standardized management mode, etc., the purpose of this paper is to promote the high-quality and high-efficiency of scientific research and production by improving the management of laboratories in oil-field scientific research units, it provides guarantee for Xinjiang oilfield to create greater economic benefits.

Against the backdrop of increasingly collaborative global scientific research, regional joint laboratories have gradually gained attention as important platforms for improving research efficiency and transforming achievements. As an effective platform for integrating regional research resources and promoting deep integration of industry, academia, and research, research consortia can play a key role in enhancing regional innovation capabilities of regional joint laboratories and accelerating the transformation of scientific and technological achievements. This article aims to explore the construction and open sharing strategy of regional joint laboratories based on research consortia, analyze the challenges and opportunities faced in the current construction and operation of regional joint laboratories, and propose corresponding countermeasures and suggestions.

Scientific research and experimental teaching are the main functions of university laboratory. Therefore, university laboratory plays an important role in personnel training and scientific research and development. However, many kinds of chemicals are stored in university laboratories, and some of them have the characteristics of being flammable and explosive, which increases the risk of accidents in university laboratories and easily threatens the safety of students and teaching staff, therefore, colleges and universities should pay attention to the laboratory safety management and control work, timely detection of security risks, improve laboratory safety. This paper describes the current situation of laboratory management in colleges and universities, and puts forward some safety control measures to provide reference for laboratory management.

Objective In this paper, aiming at the shortcomings of the industry standard of GA/T 1522-2018 Forensic Science Shooting Residue Inspection Scanning Electron Microscopy/X-Ray Spectroscopy, which only provides a few reference characteristic forms and has limited reference such as no reference energy spectrum diagram. Through simulation experiments, a practical reference is provided for the inspection of shooting residues. Methods Taking the shooting of 7.62 mm Type 51 bullet with Type 54 pistol as an example, shooting experiments of naked and clothed human bodies were simulated, and the collection, preparation and inspection steps of shooting residues, typical morphology and composition characteristics of shooting residues were explained in detail. Results Through simulation experiments, the scanning electron microscope morphology and energy spectrum characteristics of the shooting residues produced by the Type 54 pistol shooting the 7.62 mm Type 51 bullet were obtained. Conclusion Each gun and bullet combination has its own specific SEM morphology and energy spectrum characteristics.

Objective To evaluate the uncertainty of determination oftotal cadmium (Cd), lead (Pb) and chromium (Cr) in soil by microwavedigestion and inductively coupled plasma mass spectrometry (ICP-MS).Methods To ensure the accuracy and reliability of the detection results,the uncertainty of total cadmium (Cd), lead (Pb) and chromium (Cr) insoil was determined by microwave digestion and inductively coupledplasma mass spectrometry (ICP-MS) and according to JJF1059.1-2012Evalution and Expression of Uncertainty in Measurement the syntheticuncertainty was calculated and the extended uncertainty was obtained.Results The main sources of uncertainty were the standard curvepreparation the repeatability measurement of samples and the standardcurve fitting the contribution rates to the uncertainty of $\mathrm{{Cd}},\mathrm{{Pb}}$ and Cr were50.4%~60.3%, 18.7%~26.1% and 12.1%~20.4%. The influence of sampleweighing and resolution volume for the uncertainty was small and thecontribution rate was between 1.5%~2%, which could be ignored.Conclusion The effective measures to control uncertainty were putforward, which ensured the reliability of the experimental data andprovided the reference for the accurate representation of the heavymetal content in soil

Objective Evaluation of ochratoxin A (OTA) contamination inGejie Dingchuan Pills. Methods Eighty-six samples from sevenpharmaceutical manufacturers were analyzed for OTA content usingultra-high-performance liquid chromatography-tandem mass spectrometry.Results The method displayed excellent linearity within the range ofconcentrations ${0.1}\sim {10}\mathrm{{ng}}/\mathrm{{mL}}$ .The limits of detection and quantification were ${0.2}\mathrm{{\mu g}}/\mathrm{{kg}}$ and $1\mathrm{{\mu g}}/\mathrm{{kg}}$ ,respectively. Seventy-two samples were detected to be contaminated withOTA, resulting in an 84% positive rate. Furthermore, ten samples failedto meet the required standards, reflecting a failure rate of 12%. Theactual sample test results showed that the OTA detection rate of GejieDingchuan pills was high and the pollution was serious, which suggestedthe urgency of detecting and determining the maximum OTA limit ofproprietary Chinese medicine. Conclusion This study assesses the extentof OTA contamination in Gejie Dingchuan pills, offering a scientificbasis for future investigations of OTA maximum limits in Chineseproprietary medicines.

Objective To improve the precision and accuracy of atomicfluorescence spectrometry for the determination of total seleniumcontent. Methods This article analyzes the factors affecting thedetermination of total selenium in geochemical samples by hydridegeneration atomic fluorescence spectrometry, and explores threeinfluencing factors: sample digestion, instrument cleanliness, andcoexisting ion interference. Optimized the sample digestion process,summarized the issues that should be noted in the instrument during thesample determination process, as well as the common methods foridentifying and eliminating coexisting ion interference. Results Thefeasibility of the method has been verified through 13 national firstlevel standard reference materials, the detection limit of the method is ${0.006}\mathrm{{mg}}/\mathrm{{kg}}$ ,which is lower than the detection limit of DZ/T 0279.14-2016 (0.01mg/kg). The relative standard deviation of the test standard substancewere ${1.9}\%\sim {7.9}\%$ , and $\left|{\Delta\mathrm{{lg}}C}\right|$ were all less than 0.1 . The precisionand accuracy meet the requirements of DZ/T0011-2015 Geochemical CensusSpecification (1:50000). Conclusion Effectively solved the problem ofunstable quality in the determination of total selenium by atomicfluorescence spectroscopy, and improved precision and accuracy.

Objective Collect peanuts and soybeans in Beijing throughvarious channels, determine the content of 26 metal elements among them.Methods The sample is pre-processed by using a microwave to eliminatethe sample, the content of 26 metal elements was determined by inductivecoupling plasma body constituent. Results The detection limit and linearrange of 26 elements can meet the detection requirements, thecorrelation coefficients are above 0.9980 or more, relative standarddeviation (RSD) in the range of 1.1% to 6.8%, the average recovery rateis within the range of 88.8% to 108.7%. In general the content of $\mathrm{K}$ , Ca, and $\mathrm{{Fe}}$ in soybeans far exceedspeanuts, Al in ordinary soybeans is significantly higher than organicsoybeans, the $\mathrm{{Fe}}$ of redpeanut peanuts is higher than white peanut peanuts. $\mathrm{{Pb}},\mathrm{{Cd}},\mathrm{{Cr}}$ with national standards of food safety have not exceeded the standard.Conclusion Through this method, it can be effectively used for themeasurement of 26 metal elements in peanuts and soybeans.

Objective To evaluate the early diagnosis and prognosis ofmultiple myeloma (MM) by gene sequencing technology. Methods 68 patientswith MM admitted from May 2020 to May 2024 were randomly divided intoobservation group (34 cases) and control group (34 cases). Patients inthe observation group received early diagnosis and prognosis assessmentrelated to gene sequencing, while those in the control group receivedtraditional diagnosis methods. Results In the early diagnosis accuracy,the number of correct and wrong diagnoses in the observation group was31 cases and 3 cases, respectively, and the diagnosis accuracy was91.2%. The number of correct and wrong diagnoses in control group was 23cases and 11 cases, respectively, the diagnostic accuracy was ${67.62}\%$ , and the observation group washigher $\left({P <{0.05}}\right)$ .In the observation group, the number of ISS stage I, ISS Stage II, ISSStage III and high-risk mutation detection were 10 cases, 15 cases, 9cases and 12 cases, respectively, and the accuracy of prognosisassessment was 85.3%. In the control group, there were 8 cases of ISSstage I, 13 cases of ISS stage II, 13 cases of ISS stage III and 8 casesof high-risk mutation detection, respectively. The accuracy of prognosisassessment was ${67.6}\%$ , and theaccuracy of prognosis assessment in the observation group wassignificantly higher than that in the control group. The overallsurvival rate and progression-free survival rate were 94.1% and 70.6% inthe observation group, and 82.4% and 52.9% in the control group,respectively, which were higher in the observation group $\left({P <{0.05}}\right)$ . Conclusion Gene sequencing technology is helpful to the early detection of MM andthe formulation of personalized treatment plan, improve the survivalrate and quality of life of patients, and has the value of applicationand promotion in clinical practice.

Objective To analyze the effectiveness of enzyme-linkedimmunosorbent assay (ELISA) and colloidal gold test in the detection ofhuman immunodeficiency virus (HIV) antibodies. Methods From January 2022to January 2024,50 cases of sentinel surveillance and contact populationof confirmed AIDS patients in our unit were selected as the observationtarget, and they were detected by ELISA and colloidal gold testrespectively, and the difference of patients’ detection results wasanalyzed. Results Among the 50 key populations, 36 were ultimatelydiagnosed and 14 were negative, which is the gold standard for thisstudy. The diagnostic effect of enzyme-linked immunosorbent assay issignificantly better than that of colloidal gold assay, and thedifference is statistically significant $\left({P <{0.05}}\right)$ . Conclusion The enzyme-linked immunosorbent assay (ELISA) is significantly moreeffective in HIV antibody detection than colloidal gold assay, and isworthy of being the preferred detection method.

Objective To systematically evaluate the applicationpotential of ${35}\mathrm{{kV}}$ modified polypropylene in the field of wire and cable. Methods Themechanical properties, thermal properties and electrical insulationproperties of the material were investigated by differential scanningcalorimetry (DSC), tensile test and electrical property evaluation.Results The modified polypropylene showed excellent mechanical strengthand thermal stability, and the performance of electrical insulation alsomet the high standards of industrial applications. Conclusion Thesystematic test of this study not only provides a scientific basis forfurther modification and optimization of polypropylene materials, butalso lays a solid foundation for its future market promotion, andprovides important theoretical and practical value for promoting thetechnological progress of wire and cable materials and realizing thedevelopment of environmentally friendly materials.

The article delves into real-time data processing methods for monitoring tool wear in computer numerical control (CNC) machine tools, with the aim of improving the overall accuracy of the tool monitoring system. The article also systematically reviews several mainstream data processing methods, including power spectrum analysis, wavelet transform, artificial neural network technology, and multi-sensor information fusion technology. Although these methods have their own advantages and disadvantages, a single method often fails to achieve ideal results when dealing with data in complex and changing processing environments. To overcome this challenge, this paper proposes a multi-sensor information fusion data processing method based on hybrid intelligent algorithms

Objective To explore the pathogenic microbial detection results and drug susceptibility results of children with bacterial diarrhea after the collection of stool samples. Methods From April 2020 to June 2023,170 children with bacterial diarrhea participated in the study. The family members were informed about the study, and assisted the children to complete the collection of stool specimens. All the specimens were sent to the laboratory for pathogen culture, counted the distribution of pathogens, and analyzed the drug resistance of pathogens. Results 170 stool samples isolated 122 pathogenic strains, The detection rate of gram-negative bacteria was 76.23%, mainly Salmonella (27.87%), diarrheagenic Escherichia coli (21.31%), Shigella (21.31%); The detection rate of gram-positive bacteria was 23.77%, mainly Staphylococcus aureus (22.95%); Most resistance to tetracycline (41.18%), ciprofloxacin (38.24%), ampicillin (35.29%), The greatest resistance to ciprofloxacin (46.15%), tetracycline (38.46%), of ciprofloxacin (53.85%), tetracycline (46.15%), Staphylococcus aureus had to clindamycin (53.57%) and penicillin (53.57%). Conclusion Salmonella, Escherichia coli, Shigella and Staphylococcus aureus are the main causes of bacterial diarrhea in children. The drug resistance and the choice of sensitive drugs are conducive to the early recovery of children.

Objective To compare the application effects of different influenza virus detection methods. Methods 100 influenza patients admitted to a certain hospital from January 2021 to June 2023 were randomly divided into an observation group (fluorescence RT-PCR test) and a control group (colloidal gold immunochromatographic test), with 50 patients in each group. Results Compared with the control group, the observation group had a higher positive rate of testing (P&lt;0.05). Conclusion Using fluorescence RT-PCR to detect influenza virus can achieve higher detection results and improve the positivity rate of the test.

Objective To determine the inhibitory effect of skin resident bacteria under the action of different preservatives. Methods Combined with the collected real-time data, the minimum inhibitory concentration value (MIC) was calculated first, the initial constraint conditions were set, and then the bacteria were cultured. The change state of the number of colonies was compared in multiple cycles, and the average value of colony change was calculated to determine and analyze the treatment effect of the bacteria at this time. The test solution was diluted batch by batch, and the inhibition and killing pairs of bacteria were calculated according to the comparison of four test targets: aqueous solution, paste cosmetics, powder cosmetics and liquid cosmetics. Results Under different sample concentration, the resident bacteria of skin were killed and inhibited. After multi-level calculation, the final killing pair value was higher, which could reach more than 7.5. Conclusion The inhibitory effect of increasing the concentration of preservatives on resident bacteria in the skin is basically in a positive change relationship. The higher the concentration of preservatives, the better the inhibitory effect will be formed; conversely, the lower the concentration of preservatives, the relatively poor inhibitory effect will be formed.

Objective By analyzing the original detection results of urine organic acids in Changchun, Jilin Province, this study aims to explore the incidence of genetic metabolic diseases related to urine organic acid detection projects in Changchun, Jilin Province, and analyze the detection data. Methods Gas chromatography-mass spectrometry (GC-MS) was used to analyze the results of the urine organic acid detection project submitted by Jilin Jincheng Medical Co., Ltd. in Changchun, Jilin Province from January 2019 to May 2023. Results there were 83 cases of positive diseases in the initial screening, with a positive rate of 3.93%. Among them, there were 43 cases of methylmalonic acidemia with a positive rate of 51.81%, 10 cases of hyperphenylalaninemia with a positive rate of 12.05%, 6 cases of glutaric acidemia type 1 with a positive rate of 7.23%, 5 cases of Citrin protein deficiency with a positive rate of 6.02%, 5 cases of propionaemia with a positive rate of 6.02%, 4 cases of maple diabetes with a positive rate of 4.82%, and 10 cases of other diseases with a positive rate of 12.05%. Conclusion Organic acid metabolism disease, also known as organic aciduria, is the most common genetic metabolic disease. Urine organic acid screening is an effective method for preventing and treating certain genetic metabolic diseases after birth, among which methylmalonic acidemia is the most common genetic metabolic disease with abnormal organic acid metabolism. In order to improve the survival rate and quality of life of children, people must raise awareness, detect, diagnose and treat them early.

Objective To investigate the accuracy of chemiluminescenceimmunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) in thedetection of serum markers in patients with HBV infection, and toanalyze the quantitative correlation between serum markers and hepatitisB virus deoxyribonucleic acid (HBV-DNA). Methods A total of 86 patientswith hepatitis B admitted to our hospital from January 2023 to January2024 were selected as subjects. Two immunological detection methods wereused to detect HBV serum markers, and the accuracy of the two detectionmethods was analyzed. Real-time fluorescent polymerase chain reaction(qRT-PCR) was used for quantitative analysis of HBV-DNA load. McNemar’stest was used to analyze the quantitative correlation between serummarkers and HBV-DNA. Results The positive rates of HBsAg, HBsAb andHBeAg were moderate, but the positive rates of HBeAb and HBcAb werestrong. With ELISA as the gold standard, the sensitivity and specificityof CLIA assay were 97.30% and 75.00%, while with CLIA as the goldstandard, the sensitivity and specificity of ELISA assay were 96.00% and81.82%. Among the 6 serum models, the positive rate of HBV-DNA was ${95.65}\%$ , and the mean quantity ofHBV-DNA was ${1.12}\times {10}^{8}$ copies/mL, which was significantly higher than that of the other 5models. The positive rate of HBV-DNA in Xiaosanyang was also relativelyhigh (65.63%). Conclusion The two methods have strong consistency indetecting serum markers of HBV infection, but the

Lactic acid and its derivatives are important flavor substances in Nongxiang Baijiu which play a vital role in the aroma and taste of the wine. In order to screen lactic acid bacteria, this study tooke a Nongxiang Baijiu fermented grains as the research object. The isolation conditions of lactic acid bacteria were optimized, the effects of different DNA extraction methods were compared, and the species of lactic acid bacteria were identified by enzyme digestion and DNA sequencing. The results showed that 95 strains of lactic acid bacteria were preliminarily screened in MRS broth medium under anaerobic culture at 37°C for 24 h. The optimal method for DNA extraction of lactic acid bacteria was determined to be boiling method, and the strains were identified as Lactobacillus panis, which had the effects of producing lactic acid and n-propanol. This study has important reference value for the production and quality improvement of Nongxiang Baijiu.

Objective To compare the role of enzyme-linkedimmunosorbent assay and toluidine red unheated serum test in thediagnosis of syphilis. Methods 78 suspected syphilis patients in ourhospital were analyzed from January 1st, 2023 to December 31st, 2023,enzyme linked immunosorbent assay and toluidine red unheated serum testwere performed on all patients, and the testing procedures were strictlyfollowed according to the instructions. Compare the diagnosticsensitivity, specificity, and accuracy of enzyme-linked immunosorbentassay (ELISA) and toluidine red unheated serum test based on the resultsof syphilis Treponema pallidum gelatin particle agglutination test.Results The sensitivity, specificity, and accuracy of the enzyme-linkedimmunosorbent assay were 93.24%, 75%, and 92.31%, respectively. Thesensitivity, specificity, and accuracy of the toluene red unheated serumtest were 82.43%, 50%, and 80.77%, respectively. The toluene redunheated serum test was lower than the enzyme-linked immunosorbentassay, with a difference of $P<{0.05}$ . Conclusion Enzyme linked immunosorbent assay(ELISA) has higher sensitivity and accuracy in syphilis testing comparedto the non heated serum test with toluidine red, and is worthy ofattention and promotion.

Objective To study the preparation technology anddetermination of compound tetrahydropalmatine pain relief gel. MethodsOrthogonal test was performed to determine the prescription ratio of thegel. RP-HPLC method was adopted. The determination was performed onEclipce XDB-C18 ( ${200}\mathrm{\;{mm}}\times {4.6}\mathrm{\;{mm}},{5\mu}\mathrm{m}$ ) column with mobile phase consisted ofmethanol-0.6% glacial acetic acid (pH 6.0) 30 : 70 . The detectionwavelength was ${280}\mathrm{\;{nm}}$ , the flow rate was $1\mathrm{\;{mL}}/\mathrm{{min}}$ .Results With $1\mathrm{\;g}$ carbomer ${940},{20}\mathrm{\;g}$ propyleneglycol, $8\mathrm{\;g}$ glycerol, ${0.5}\mathrm{\;g}$ triethanolamine as thegel matrix, the linear ranges of tetrahydropalmatine and imperatorinwere ${16.1}\sim {96.6}\mathrm{{\mug}}/\mathrm{{mL}}\left({r ={0.9999}}\right)$ and ${10.4}\sim {62.4}\mathrm{{\mug}}/\mathrm{{mL}}$ $\left({r={0.9999}}\right)$ , respectively. The average recoveries were99.13%(RSD was 0.79%) and 99.26%(RSD was 0.82%), respectively.

Objective To add data output and processing functions to ordinary models of UV visible spectrophotometers. Methods Connect the spectrophotometer to an Android system phone using a serial port to USB cable. The phone uses serial software to receive and process the measurement data sent by the spectrophotometer. Results The connection between the spectrophotometer and the mobile phone was successfully achieved. The mobile phone can smoothly receive and store the output data of the spectrophotometer, and perform graphical processing, synchronously drawing the wavelength absorbance scanning spectrum of the sample. The obtained absorption spectrum is basically consistent with the commercial instrument. Conclusion A simple modification scheme for the output and processing of ordinary spectrophotometer data based on Android system smartphones has been proposed. The modification method is low-cost, easy to operate, and ordinary UV visible spectrophotometers with serial interfaces can be upgraded and modified.

Objective To determine the content of phosphoric ester flame retardants in samples and provide a scientific basis for optimizing the flame retardancy of plastic products. Methods The phosphoric ester flame retardants were extracted from plastic samples with suitable solvent, and the content of phosphoric ester flame retardants was determined by GC-MS. Results The method is fine and can effectively remove impurity interference, can effectively remove the interference of impurities, has a good linearity with a correlation coefficient of more than 0.995; has a good precision with a relative standard deviation of less than 10%; a spiked recovery test was carried out throughout the process, and the recovery rate was 80%~120%. Conclusion This method is accurate and reliable, and can be used for the determination of the content of phosphate ester flame retardants in samples.

Objective To establish a method for the detection of cyclicaldehyde compounds in drinking water sources, in order to practice highquality development and improve the technical support of public servicequality. Methods Quantitative detection method of cyclic aldehydecompounds in drinking water by using SPME-GC-MS/MS were established.Results The results show that the optimal conditions for SPME aimed atcyclic aldehyde compounds in drinking water are an incubationtemperature of ${50}^{\circ }\mathrm{C}$ , a rotationalspeed of ${500}\mathrm{r}/\mathrm{{min}}$ , anincubation duration of ${15}\mathrm{\;{min}}$ , followed by asample extraction time of ${20}\mathrm{\;{min}}$ . The detectionlimits of this quantitative detection method were 2.87~4.67 ng/ L, therelative standard deviation were 6%~16%, the recoveries were 70.7%~115%.A specific water sample was collected to ascertain that the keyodor-causing compound was2-EDD, exhibiting concentrations ${32}\mathrm{{ng}}/\mathrm{L}$ . Conclusion This method can accurately and swiftly identify key odor-causingcompounds in drinking water.

The purpose of this paper is to discuss the application of Liquid Chromatography-Mass Spectrometry (LC-MS) in drug quality analysis in order to improve the accuracy and efficiency of drug detection. The basic principle, equipment and application of LC-MS in drug quality analysis are reviewed in this paper. LC-MS technology has shown excellent performance in the qualitative, quantitative, fingerprint and pharmacokinetics of drugs, providing an efficient and accurate tool for drug quality analysis, and has a broad application prospect.

Objective To explore the clinical application value of combined detection of echocardiography, dynamic electrocardiogram, and plate exercise test in the diagnosis of coronary heart disease. Methods 70 suspected coronary heart disease patients were selected and subjected to plate exercise test, dynamic electrocardiogram, echocardiography, and coronary angiography. Analyze the diagnostic performance of each method through ROC curve, and calculate sensitivity, specificity, and AUC values. Results The individual detection AUC values of the three methods were 0.796 (plate exercise test), 0.737 (dynamic electrocardiogram), and 0.773 (echocardiogram), respectively, and the combined detection AUC was 0.826. Conclusion Combined detection can significantly improve the diagnostic accuracy of coronary heart disease, with higher sensitivity and specificity than single detection. It is recommended to widely apply it in clinical practice.

The development of intelligent sensor technology has brought revolutionary changes to all walks of life, especially in the field of industrial production and textile testing to show great potential. Smart sensors are not only capable of sensing physical or chemical quantities in the environment, but also have data processing and intelligent analysis capabilities, making them a significant advantage in textile defect detection. This paper analyzes the basic principle, characteristics and application status of intelligent sensor in industrial production and textile testing. The applications and advantages of different types of intelligent sensors, such as vision, infrared and ultrasonic systems, in textile production are analyzed.

Objective To explore the clinical significance of thecombined detection of fasting blood glucose and glycosylated hemoglobinin the diagnosis and treatment of diabetes. Methods From January 2023 toJanuary 2024,50 healthy people in our hospital were selected as thecontrol group, and 50 diabetes patients in our hospital at the same timewere selected as the observation group. Compare the fasting bloodglucose (FPG), glycated hemoglobin (HbA1c) levels, HbA1c, FPG, and oralglucose tolerance test (OGTT) blood glucose test results, combined andsingle test results after 2 hours of OGTT glucose administration, aswell as the correlation between HbA1c and FPG in the observation group.Results The blood glucose levels, HbA1c, and FPG of the observationgroup after 2 hours of OGTT administration were higher than those of thecontrol group $\left({P<{0.05}}\right)$ . The observation group showed a positivecorrelation between HbA1c and FPG $(r={0.608}$ , $P <{0.05}$ ).The specificity, sensitivity, and accuracy of HbA1c and FPG combineddetection are superior to single detection $\left({P <{0.05}}\right)$ . Conclusion The combined detection of HbA1c and FPG has high diagnostic accuracy fordiabetes, reduces the rate of misdiagnosis and missed diagnosis, canspeed up the diagnosis of diabetes, is conducive to early treatment, andis worth promoting.

Multi-angle phased array ultrasonic technology has important application value in fatigue crack detection of complex structural parts. In this paper, the basic principle of the technique is summarized first, and then the main difficulties in fatigue crack detection of complex structural parts are analyzed, including complex geometric structure interference, quantitative crack characteristics evaluation and environmental factors. To address these challenges, innovative solutions such as deep learning signal processing, multi-parameter feature extraction and adaptive detection strategies are proposed. This study provides a new idea and direction for the further development of fatigue crack detection technology for complex structural parts.

Objective To analyze the application results offluorescence quantitative reverse transcription-polymerase chainreaction (RT-PCR) in the detection of pathogenic microorganisms. MethodsA retrospective analysis was made of 7502 microbiological test dataexamined in the laboratory of our hospital from January 14th, 2023 toDecember 14th, 2023. Conventional RT-PCR method and fluorescentquantitative RT-PCR method were performed respectively, and the goldstandard was the results of special staining microscopy. The diagnosticefficiency and other indicators of the two test results were analyzed.Results ① The ratio of negative to positive by gold standard was ${490}: {7012}$ , the ratio of negative topositive by conventional RT-PCR was ${479}:{6358}$ , and the ratio of negative to positive by fluorescencequantitative RT-PCR was 488 : 7010. ② The sensitivity, specificity andaccuracy of fluorescence quantitative RT-PCR method were 99.97%, 99.59%and 99.95%, which were higher than that of conventional RT-PCR method,with statisticalsignificance $\left({P<{0.05}}\right)$ . ③ The detection rates of variousmicroorganisms by fluorescence quantitative RT-PCR were 99.91%, 100.00%,100.00%, 99.91%, 100.00%, 100.00%, 99.97%, higher than that byconventional RT-PCR, with statistical significance ( $P <{0.05}$ ). Conclusion Fluorescencequantitative RT-PCR method has high diagnostic efficiency and detectionrate in pathogenic microorganisms, which can provide scientific basisfor doctors’ diagnosis and treatment and can be implemented.

Objective Establishment of a capillary column headspacesampling program for Gas chromatography determination of ethanol residuein Isosorbide Mononitrate sustained-release tablets. Methods A capillarycolumn $\left({{30}\mathrm{\;m}\times {0.320}\mathrm{\;{mm}},{0.25\mu}\mathrm{m}}\right)$ with $6\%$ cyanopropylphenyl-94%dimethylpolysiloxane (or similar polarity) as stationary solution wasused. The starting temperature was ${50}^{\circ }\mathrm{C}$ and maintainedfor $6\mathrm{\;{min}}$ , thetemperature of the detector was ${250}^{\circ }\mathrm{C}$ , thetemperature of the inlet was ${200}^{\circ }\mathrm{C}$ , theequilibrium temperature of the headspace bottle was ${80}^{\circ }\mathrm{C}$ , the equilibriumtime was ${30}\mathrm{\;{min}}$ , theflow rate of nitrogen was $1\mathrm{\;{mL}}/\mathrm{{min}}$ , and thetemperature of the headspace bottle was ${200}^{\circ }\mathrm{C}$ . The shuntratio was ${50}: 1$ . Results Underthe above chromatographic conditions, the retention time of ethanol was $3\mathrm{\;{min}}$ and theconcentration of ethanol was in the range of ${0.43}\sim {10.8\mu}\mathrm{g}/\mathrm{{mL}}$ . The recoveries were ${87.04}\%\sim {94.45}\%,\mathrm{{RSD}}={0.17}\%\left({n = 6}\right)$ .

Objective Explore the method of using gas-phase molecularabsorption spectroscopy to determine the ammonia content in unorganizedexhaust gas emissions. Methods Explore the detection method from theaspects of detection limit, calibration curve, precision and accuracy,and compare it with the national standard method. Results Theexperimental results showed that there was a good linear relationshipbetween ammonia concentration and absorbance within the range of $0 \sim 2\mathrm{{mg}}/\mathrm{L}$ , with acorrelation coefficient of 0.9999 . The detection limit of the methodwas ${0.1}\mathrm{{\mug}}/{10}\mathrm{\;{mL}}$ . By measuring the standard sample andconducting spiked recovery tests on the actual sample, the relativestandard deviation RSD of the experimental results was ${0.3}\%\sim {1.5}\%$ , the relative errorwas $-{0.1}\%\sim {0.6}\%$ , and thespiked recovery rate was ${97.1}\%\sim {99.5}\%$ . Compared with thenational standard method, there is no significant difference in theresults obtained by this method. Conclusion This method does not requirepre-treatment, does not use highly toxic reagents, has stronganti-interference ability, and can accurately detect the content ofammonia in the unorganized discharge sample. Suitable for determiningthe ammonia content in unorganized emission samples