Objective To analyze the application results offluorescence quantitative reverse transcription-polymerase chainreaction (RT-PCR) in the detection of pathogenic microorganisms. MethodsA retrospective analysis was made of 7502 microbiological test dataexamined in the laboratory of our hospital from January 14th, 2023 toDecember 14th, 2023. Conventional RT-PCR method and fluorescentquantitative RT-PCR method were performed respectively, and the goldstandard was the results of special staining microscopy. The diagnosticefficiency and other indicators of the two test results were analyzed.Results ① The ratio of negative to positive by gold standard was ${490}: {7012}$ , the ratio of negative topositive by conventional RT-PCR was ${479}:{6358}$ , and the ratio of negative to positive by fluorescencequantitative RT-PCR was 488 : 7010. ② The sensitivity, specificity andaccuracy of fluorescence quantitative RT-PCR method were 99.97%, 99.59%and 99.95%, which were higher than that of conventional RT-PCR method,with statisticalsignificance $\left({P<{0.05}}\right)$ . ③ The detection rates of variousmicroorganisms by fluorescence quantitative RT-PCR were 99.91%, 100.00%,100.00%, 99.91%, 100.00%, 100.00%, 99.97%, higher than that byconventional RT-PCR, with statistical significance ( $P <{0.05}$ ). Conclusion Fluorescencequantitative RT-PCR method has high diagnostic efficiency and detectionrate in pathogenic microorganisms, which can provide scientific basisfor doctors’ diagnosis and treatment and can be implemented.

fluorescence quantitative reverse transcription-polymerase chain reaction method  /  pathogenic microorganism  /  inspect  /  conventional reverse transcription-polymerase chain reaction method  /  mycoplasma