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Objective To established an analytical method for indirectdetermination of xylo-oligosaccharides in high gelatinization-bakedfoods by high performance liquid chromatography with refractive indexdetector. Methods After degreased with petroleum ether, the samples wereextracted with 20% ethanol solution, and macromolecular sugars such asdextrin and starch were precipitated with 68% ethanol solution. Thesugars in the extraction solution were hydrolyzed into monosaccharidesby diluted sulfuric acid, the solution before and after hydrolysis wasseparated by amide column, detected by refractive index detector, andquantified by external standard method. The product between thedifference of xylose before and after hydrolysis and the averageconversion coefficient of xylo-oligosaccharides and xylose was thexylo-oligosaccharides. Results When the xylose was ${0.25}\sim {5.00}\mathrm{{mg}}/\mathrm{{mL}}$ ,there was a good linear relationship between peak area and concentration $\left({r ={0.9998}}\right)$ . Therecovery rates ranged from 92.0% to 95.8%, the relative standarddeviations (RSDs) were ${1.8}\%\sim {4.0}\%$ in highgelatinization-baked foods. The limit of detection and limit ofquantization of xylo-oligosaccharides were ${0.03}\mathrm{\;g}/{100}\mathrm{\;g},{0.1}\mathrm{\;g}/{100}\mathrm{\;g}$ respectively. Conclusion The extraction method of low concentrationethanol solution is feasible and specific. The method avoided the impactof high gelatinization on the low extraction efficiency and measurementresults. It can determine xylo-oligosaccharides in highgelatinization-baked foods accurately and quickly.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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