Objective To compare the role of enzyme-linkedimmunosorbent assay and toluidine red unheated serum test in thediagnosis of syphilis. Methods 78 suspected syphilis patients in ourhospital were analyzed from January 1st, 2023 to December 31st, 2023,enzyme linked immunosorbent assay and toluidine red unheated serum testwere performed on all patients, and the testing procedures were strictlyfollowed according to the instructions. Compare the diagnosticsensitivity, specificity, and accuracy of enzyme-linked immunosorbentassay (ELISA) and toluidine red unheated serum test based on the resultsof syphilis Treponema pallidum gelatin particle agglutination test.Results The sensitivity, specificity, and accuracy of the enzyme-linkedimmunosorbent assay were 93.24%, 75%, and 92.31%, respectively. Thesensitivity, specificity, and accuracy of the toluene red unheated serumtest were 82.43%, 50%, and 80.77%, respectively. The toluene redunheated serum test was lower than the enzyme-linked immunosorbentassay, with a difference of $P<{0.05}$ . Conclusion Enzyme linked immunosorbent assay(ELISA) has higher sensitivity and accuracy in syphilis testing comparedto the non heated serum test with toluidine red, and is worthy ofattention and promotion.

syphilis  /  enzyme linked immunosorbent assay  /  toluidine red unheated serum test  /  sensitivity