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Objective To evaluate of uncertainty in the determination of residual cyclopropamide in animal food by high-performance liquid chromatography. Methods The sample was repeatedly extracted by the mixture of Trichloroacetic acid and acetonitrile. The supernatant was degreased with n-hexane, and then concentrated by rotating evaporation in a water bath. The mixture of trichloroacetic acid and acetonitrile was redissolved, and then purified by a mixed cation exchange solid phase extraction column. After separation by an amino chromatographic column, acetonitrile water was used as the mobile phase for isocratic elution. The residue of cyclopromazine in animal food was quantitatively determined by diode array detector and external standard method. According to the JJF 1135-2005 Chemical Analysis Measurement Uncertainty Assessment and JJF 1059.1-2012 Measurement Uncertainty Assessment and Representation, the uncertaintygenerated during the entiretesting process includes weighing, standard, standard working curve formulation, standard curve fitting, sample measurement repeatability, instrument and standard recovery rate, and the uncertainty introduced by each factor is calculated, and finally theexpansionuncertainty is obtained. Results When the detection result of cyromazine in chicken was 19.8 µg/kg, with an expanded uncertainty of 3.2 µg/kg. Conclusion The main factors affecting uncertainty are the determination of standard, samples, the preparation of standard curves and the fitting of standard curves.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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