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Objective To investigate the accuracy of chemiluminescenceimmunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) in thedetection of serum markers in patients with HBV infection, and toanalyze the quantitative correlation between serum markers and hepatitisB virus deoxyribonucleic acid (HBV-DNA). Methods A total of 86 patientswith hepatitis B admitted to our hospital from January 2023 to January2024 were selected as subjects. Two immunological detection methods wereused to detect HBV serum markers, and the accuracy of the two detectionmethods was analyzed. Real-time fluorescent polymerase chain reaction(qRT-PCR) was used for quantitative analysis of HBV-DNA load. McNemar’stest was used to analyze the quantitative correlation between serummarkers and HBV-DNA. Results The positive rates of HBsAg, HBsAb andHBeAg were moderate, but the positive rates of HBeAb and HBcAb werestrong. With ELISA as the gold standard, the sensitivity and specificityof CLIA assay were 97.30% and 75.00%, while with CLIA as the goldstandard, the sensitivity and specificity of ELISA assay were 96.00% and81.82%. Among the 6 serum models, the positive rate of HBV-DNA was ${95.65}\%$ , and the mean quantity ofHBV-DNA was ${1.12}\times {10}^{8}$ copies/mL, which was significantly higher than that of the other 5models. The positive rate of HBV-DNA in Xiaosanyang was also relativelyhigh (65.63%). Conclusion The two methods have strong consistency indetecting serum markers of HBV infection, but the
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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