Objective To evaluate the performance of HORIBA ABX Yumizen H550 automatic blood cell analyzer. Methods According to the national health industry standard Quality Requirements for Analysis of Routine Items in Clinical Hematology Examination (WS/T 406-2012), the automatic blood cell analyzer was evaluated for background counting, carrying contamination, in-batch precision, linearity, accuracy, comparability of results in different suction modes, and comparability of results in the laboratory (the reference instrument was Sysmex XS800i automatic blood cell analyzer). Results The detection values of background count of all items of the automatic blood cell analyzer were met the requirements of industry standards and manufacturer’s instrument manuals, and the carryovers of white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (HGB) and blood platelet (PLT) count of that were all passed. The results of within-run precision and inter-day precision were less than the requirements of the specifications of instrument and were far less than the requirements of standard WS/T 406-2012. Within the linearity range, the slopes of the linear regression equation were within the range of$1 \pm {0.05}$. The correlations coefficient(r)of these equations were larger than 0.975 . The pass rate that the results compared with that of Sysmex XS800i were larger than 80%. The compared results of intra clinical laboratory indicated the trueness of instrument, the outcome bias between different suction patterns. Conclusion Yumizen H550 automatic blood cell analyzer has favorable performance, accurate and reliable results. It’s an ideal automatic blood cell analyzer.

Objective The structure and composition of Shoushan tianhuang were determined, and in order to understand the reason why field-yellow stone is different from other varieties of koreite. Methods It’s structure was observed by microscope and scanning electron microscope, and it’s chemical composition was analyzed by near infrared spectrometer, X-ray powder diffractometer and ICP-MS. Results By scanning electron microscopy, it was found that the crystal structure of fShoushan tianhuang is aphanocrystalline, the crystal is lamellar, the particle size is uneven, the overall orientation is poor, there is idiomorphic or hemidiomorphic pyrite and the particle boundary is clear. Comparing and analyzing the data, it is found that$\mathrm{{Cr}}$element is stable and there are three kinds of mineral composition in Shoushan tianhuang: illite is the main mineral. The main mineral is the dickite and the secondary mineral is the illite. The main minerals are dickite and kaolinite. Conclusion The analysis of structure and chemical composition of Shoushan tianhuang is of great value for identifying varieties of koreite and distinguishing different mining areas, and also provides important data support for understanding the production state and particularity of Shoushan tianhuang.

Against the backdrop of increasing demands for quality elements such as reliability and safety in FPGA software for infrared and optoelectronic payloads, the establishment of a corresponding FPGA software testing laboratory is particularly important. This paper aims to build and improve the capability management system of the FPGA software testing laboratory to obtain recognition from the China National Accreditation Service for Conformity Assessment (CNAS). This paper elaborates in detail on the construction of the laboratory management system using the Plan-Do-Check-Act (PDCA) quality method, shares case studies and outcomes of laboratory construction, and provides ideas and references for the construction of CNAS competency management systems in other laboratories.

This study aims to explore effective control methods for testing standards in medical device testing laboratories, in order to improve the reliability and consistency of testing results. The research adopts a combination of literature analysis and measure analysis to systematically sort out the key links of detection standard control. Research has shown that taking comprehensive measures is crucial for controlling testing standards, significantly improving the quality and credibility of medical device testing, and providing strong support for ensuring patient safety and promoting industry development.

For China's booming robot market, robot testing is a key link to ensure the performance and quality of robots, and quality control methods are also crucial for robot testing laboratories. In view of the problems faced by quality control methods in the application of robot testing laboratories, this paper analyzed and discussed the strategies of robot testing laboratories in the application of quality control methods to control testing quality, and effectively improved the accuracy and reliability of testing data and ensured the quality of robot products through the scientific and planned implementation of quality control.

Geotechnical compaction test is an important means to determine the relationship between the optimal dry density and the optimal water content of soil under a certain compaction work, which can be used to understand the compaction performance of soil, and has important significance to ensure the quality of engineering foundation construction. On the basis of comprehensive analysis of influencing factors of geotechnical compaction test, combined with the author's engineering practice experience, this paper puts forward strategies to strengthen the control of the authenticity and effectiveness of geotechnical compaction test results, aiming at improving the quality and efficiency of geotechnical tests and ensuring the reliability and functionality of geotechnical tests.

University laboratories play an increasingly important role in enhancing the ability of scientific research and innovation, ensuring the quality of teaching, promoting interdisciplinary integration and promoting the sustainable development of the university. However, at present, the management of university laboratory is faced with some problems, such as imperfect management system, outdated facilities, weak talent team, lack of open sharing and lagging information level. This paper proposes to implement 6S management model to clarify the responsibility system, increase investment to accelerate the upgrading of facilities, strengthen the construction of talent team to optimize the management structure, expand open and sharing channels to improve resource utilization efficiency, deepen information construction to improve the intelligent level of management and other coping strategies. By analyzing successful cases, we summarize experiences and lessons. In order to provide reference and reference for university laboratory management.

With the rapid development of information technology, commodity barcode, as an effective information carrier, plays an increasingly important role in laboratory quality control. This paper discusses how barcode standardization can improve the efficiency and accuracy of product quality inspection through an in-depth analysis of the basic composition and function of commodity barcodes and their application in the laboratory. This paper focuses on the role of commodity barcodes in improving data traceability, optimizing quality monitoring processes, and enhancing product quality compliance, and demonstrates the practical application effect of commodity barcode standardization in laboratory quality control through specific case analysis. The results show that the standardization of commodity barcodes is one of the key technologies to improve the efficiency and quality management level of laboratories.

In order to achieve real-time monitoring of the status of fire-fighting equipment, improve detection efficiency and real-time data, this paper mainly studies the remote detection of fire-fighting products using advanced network data collection and security technologies. The remote detection system for fire protection products faces security risks in data transmission and storage, including data theft, tampering, and network attacks. It must apply encryption technology, identity authentication, multi factor authentication, firewalls, and intrusion detection systems. In addition, it is necessary to comply with data privacy regulations and perform data anonymization and de identification processing to protect user privacy, which is of great significance for achieving remote detection of fire protection products.

In view of the problems of laborious personnel management, difficult object control and inadequate infrastructure conditions in the safety work of university laboratories, and based on the construction concept of "harmonious and safe campus", and combined with the current situation of safety management in the material laboratories of our college, we propose to comprehensively promote the informatization of laboratory safety management in four aspects: laboratory safety education, open booking of laboratories and equipment, purchase and supervision of hazardous chemicals, and laboratory safety risk assessment. By using information technology to reduce unsafe human behaviour, eliminate unstable physical conditions and make up for shortcomings in infrastructure construction, we provide new solutions to the difficulties of laboratory safety management.

Objective To improve the determination of free silica content in dust by pyrophosphoric acid heated by silicon carbide in order to ensure the accuracy of detection. Methods Analyze the influencing factors and controls during the experimental process, including the determination of dust sample composition, sample processing, preparation of pyrophosphate, control of heating temperature, hot filtration, ashing and weighing. Results By controlling and improving the influencing factors during the experimental process, measurement errors can be effectively avoided. Conclusion The importance of determining the characteristics of dust samples during the measurement process lies not only in the preparation of pyrophosphate and strict temperature control, but also in the accuracy of data analysis for quality control sample analysis.

With the needs of national scientific research and teaching in the new era, the types of chemicals in university laboratories are gradually increasing, and the treatment of laboratory waste liquid has attracted more and more people's attention. Improper treatment methods will bring harm to the surrounding environment and even people's healthy life. Article combined with the existing laboratory waste liquid source management is not standard, waste liquid disposal channel is not open, compare three common laboratory waste liquid disposal way, draw lessons from the advanced experience of foreign university waste liquid disposal, it is pointed out that it is necessary to clarify the main responsibility, strengthen source management, strengthen specification consciousness, through process management, multilateral coordination, dredge waste disposal way to achieve laboratory waste liquid feasible, easy, legal, compliance emissions.

In the field of scientific research, the optimization of laboratory resource management is one of the key factors in enhancing research efficiency. This paper provides an in-depth analysis of the classification and current configuration of laboratory resources, exploring the relationship between resource management models and research efficiency. Systematic resource management not only optimizes the efficiency of scientific equipment and human resources but also enhances the efficiency of research teams.

Objective To observe the test results and substantial impact of real-time fluorescence quantitative PCR detection technology in detecting hepatitis B virus DNA. Methods 1500 patients with hepatitis B from January 2023 to December 2023 were selected for the study data, and all of them were detected by serological markers and real-time fluorescent quantitative PCR. The control standard was the clinical diagnosis results, and the comparison index was the DNA quantitative detection results. Results Among the 1500 confirmed hepatitis B patients, real-time fluorescence quantitative PCR technology detected hepatitis B virus DNA quantification results, which showed that the viral load of 1380 patients (92.00%) could be clearly quantified, while the serological marker method could only clearly quantify the viral load of 1260 patients (84.00%). In addition to technology, factors that may affect the final test results include laboratory operations, sample and nucleic acid extraction, and storage at multiple stages. Conclusion Real time fluorescence quantitative PCR technology has higher sensitivity and accuracy compared to traditional serum blood marker detection methods, and is more suitable for modern laboratory medicine.

Testing laboratory resources are an important support for teaching and scientific research in universities. How to evaluate the utilization efficiency of testing laboratories through big data analysis and make targeted optimization suggestions is of great significance to improving the testing capabilities and resource utilization efficiency of universities. Based on the big data analysis method, this article starts from the aspects of testing laboratory usage data collection, storage, analysis, visualization, etc., builds a testing laboratory usage efficiency evaluation model, and proposes an optimization strategy. The study found that the evaluation of testing laboratory usage efficiency needs to comprehensively consider multi-dimensional indicators such as frequency of use, duration, equipment utilization, sample processing capacity, testing accuracy, testing cycle, etc. Data collection and processing are key links. In response to the problems discovered in the assessment, optimization measures such as strengthening planning management, improving the reservation system, optimizing layout, and establishing incentive mechanisms were proposed. This study has certain reference value for promoting the informatization of testing laboratory management and improving testing capabilities and efficiency.

This study aims to develop a rapid detection method based on multiplex real-time quantitative PCR technology for accurate identification and quantification of common pathogenic bacteria on face masks, to assess the hygienic condition of masks and control nosocomial infections. Methods Using TaqMan probe-based real-time fluorescence quantitative PCR as the core technology, specific primers and fluorescently labeled probes were designed and optimized for simultaneous quantification of specific gene sequences from three pathogens through one-tube multiplex detection. The experiment began by screening specific target genes and sequences from the NCBI database and designing primers and probes. Subsequently, quality testing of primers and probes was conducted, and standard samples were prepared through amplification of specific sequences and TA cloning. Finally, standard curves were established, and mask samples were tested. Results After optimizing experimental conditions, an efficient and stable multiplex real-time quantitative PCR detection system was successfully established. The detection efficiency of standard samples for three pathogens was evaluated at different concentration gradients. The detection data showed that the primers and probes had high specificity for target pathogens, and the amplification efficiency and signal intensity met experimental requirements. In the detection of mask samples, the method accurately quantified the genomic DNA copy numbers of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus hemolyticus, thereby assessing the degree of microbial contamination on masks. Conclusion The rapid detection method for pathogenic bacteria on masks based on multiplex real-time quantitative PCR technology demonstrates high sensitivity, specificity, and speed, providing strong technical support for clinical and public health

This article summarizes the non-destructive testing techniques for concrete structures and their importance in evaluating the durability of concrete. With the aging of infrastructure, the demand for rapid and accurate evaluation and maintenance of concrete structures is increasing. Non destructive testing technology is highly valued for its ability to provide accurate evaluations without damaging the structure. The article introduces the main non-destructive testing methods, including ultrasonic testing, electromagnetic wave testing, and radiometric testing, and discusses in detail the application effects of these technologies in practical engineering. The advantages and disadvantages of each technology were also compared and analyzed. In the future, with the development of intelligence, integration, and portability, non-destructive testing technology will further improve detection efficiency and accuracy, and be widely used in long-term monitoring of infrastructure, providing technical support for extending the service life of structures and reducing maintenance costs.

Based on the current status of university laboratory culture construction, this paper deeply analyzes the existing problems. Drawing on the concept of the management model of EHS (environment, health, safety) in management science, a generalized EHS model applicable to the management of university laboratories is proposed. Furthermore, an evaluation index system model (seven-force diagram) for laboratory safety culture construction is constructed based on the AHP (analytic hierarchy process) method, which can effectively enhance experimental safety and ensure standardized management of university laboratories.

Objective To study the application of SERS technique in the quantitative detection of antibiotic residues in poultry meat and evaluate its potential in food safety monitoring. Methods The SERS principle and enhancement factor calculation were introduced, and the influence of the shape and size of nanoparticles on SERS effect was analyzed experimentally and simulated. Different concentrations of antibiotic residues were detected through SERS, and the detection sensitivity and linear response were evaluated. Results SERS was reliable at${0.8}\mathrm{{nmol}}/\mathrm{L}$antibiotic concentration, the linear response range was${0.1}\sim {1000}\mathrm{{nmol}}/\mathrm{L}$, the${r}^{2}$was 0.999, and the enhancement factor was stable at${10}^{6}$. Conclusion SERS technology has high sensitivity and good linear response, which is suitable for the detection of antibiotic residues in food safety monitoring.

Chenpi is an important Chinese herbal medicine and seasoning. Their quality and safety have drawn considerable concerns by consumers. The possible chemical risk substances such as pesticides, heavy metals and mycotoxins in Chenpi will have a negative impact on the health and safety of consumers. In order to reduce and avoid the quality and safety problems, researchers have utilized a variety of analytical techniques to develop novel analytical methods for the detection of hazardous chemical substances in Chenpi. The research progress on detection techniques for the quality and safety of Chenpi in recent years is reviewed in this paper, which is anticipating to provide a certain theoretical support and technical guidance for relevant researchers.

The safety of university laboratories is a fundamental guarantee for promoting the continuous development of education and scientific research, and for teachers and students to carry out work and study smoothly. In China's vigorous promotion of the construction of "Double First Class” universities, the existing management methods and personnel configuration are unable to meet the current national requirements for laboratory safety management in universities. Building a scientific, comprehensive, practical, and easy-to-use laboratory safety management platform is an important issue that universities urgently need to study and solve. This article analyzes the necessity of building a laboratory safety management platform in universities under the current information technology background. Based on the actual work of Xinjiang University, a laboratory safety management system construction plan is proposed, and the system operation effect is analyzed to achieve systematic and intelligent management of the laboratory. It has played a positive role in supporting the cultivation of innovative talents and standardizing laboratory management, and has achieved certain results.

Objective To explore the application effect of C-reactive protein combined with blood routine and blood culture in the diagnosis of bacteremia. Methods In this study, 69 patients with suspected bacteremia examined from May 2023 to May 2024 were selected as the observation group. In order to more intuitively understand the application value of the three combined detection methods, 69 healthy patients in the same period were selected as the experimental group. Analysis of blood routine indexes, strain distribution in blood culture and C-reactive protein detection data in both participating groups. Results In the blood cultures. Results A total of 8 strains were detected in the blood culture of 69 suspected bacteremia patients in the observation group. Among the two strains with high detection rate, except gram-negative bacteria and gram-positive bacteria, the two strains with the highest detection rate were staphylococcus 25 strains. Escherichia coli 16 granules, and 16 E. coli with detection rate of${23.19}\%$. WBC$\left({{15.45}\pm {2.18}}\right)\times {10}^{9}/\mathrm{L}$, NEU$\left({{81.24}\pm {2.84}}\right)\%$, LYM$\left({{53.24}\pm {2.68}}\right)\%$, C-reactive protein levels (53.24±2.68) mg/L, WBC (6.45±1.28)×10${}^{9}$/L, NEU (62.41±2.34)%, LYM (26.45±2.18)%, and C-reactive protein levels$\left({{6.28}\pm {1.25}}\right)\mathrm{{mg}}/\mathrm{L}$. WBC, NEU, LYM and C-reactive protein levels in patients with suspected bacteremia in the observation group were significantly higher than those of healthy patients in the experimental group$\left({P <{0.05}}\right)$. Of the 69 suspected bacteremia, 66 were positive and 3 were negative. Among them, 63 cases were positive in blood culture, 58 cases were positive in blood routine test, 56 cases were positive in C-reactive protein test, and 66 cases were positive in C-reactive protein combined with blood routine and blood culture test. Among the four detection methods, C-reactive protein combined with blood routine and blood culture test were no different with the confirmed results. Conclusion In clinical diagnosis and detection of suspected bacteremia patients, C-reactive protein combined with blood routine and blood culture can maximize the accuracy of detection data, and the combined diagnosis of the three is of higher clinical value.

Objective Through the statistical analysis of the actual detection results of Down's disease screening and the pregnancy outcome of follow-up in Zhuhai, the health economics evaluation was carried out. Methods The data of 31967 pregnant women who underwent serum screening (SS) and non-invasive prenatal testing (NIPT) during the same pregnancy in Zhuhai City from 2018 to 2021 were retrospectively analyzed. The cost-benefit analysis was conducted according to 5 different screening strategies. Results Among 31,967 pregnancies, 38 were Down syndrome babies (including 3 pregnant women aged over 35 years), with a Down syndrome incidence of 1/841. Serological screening detected 1,649 cases of high risk of T21, of which 21 were true positives, with a positive predictive value of 1.27% and a detection rate of 55.3%; NIPT screening detected 49 cases of high risk of T21, 38 cases of Down syndrome babies, with a positive predictive value of 77.55% and a detection rate of 100%. The total cost generated by calculation strategy 4 was the lowest, about 27.44 million yuan. Conclusion NIPT can be used as primary screening test instead of serological screening method in prenatal Down's screening for secondary prevention and control of birth defects, which has great social and economic benefits.

Objective This study aims to evaluate the stability and repeatability of digital pressure gauges in laboratory environments, to verify their measurement accuracy in different pressure ranges and performance in long-term use, and to provide scientific basis for the calibration and application of digital pressure gauges. Methods The experiment adopted a multi-point and multiple measurement method, selecting three different pressure points of 0.1,0.3,0.6 MPa for testing. Ten repeated measurements were taken at each pressure point to evaluate the repeatability of the digital pressure gauge. Meanwhile, through long-term measurements every three months for one year, observe the stability performance of the digital pressure gauge during long-term use. The experimental environment conditions are strictly controlled, with a temperature maintained at${\left({20}\pm 2\right)}^{\circ}\mathrm{C}$and a relative humidity between${40}\%$and${60}\%$. Results The standard deviations of the digital pressure gauge at the three pressure points were 0.10088 , 0.30057 and 0.60087, respectively. The standard deviations were small and the repeatability was good. At the same time, during long-term use, the measurement values at each measurement point have a relatively small change amplitude, and the changes in range and average values are within the allowable error range, indicating that it has good long-term stability. Conclusion Digital pressure gauges exhibit high repeatability and stability in laboratory environments, making them suitable for high-precision pressure measurement applications. This study provides a scientific basis for the calibration and practical application of digital pressure gauges, and also provides a reference direction for the research and improvement of related equipment in the future.

Objective To study error compensation techniques in high-precision pressure detection systems and improve measurement accuracy. Methods A comprehensive error compensation method based on neural networks was proposed, and a pressure sensor error model was established. An adaptive neural network compensator was designed. Results This method achieves effective compensation for nonlinear errors, and experimental results show that the systematic nonlinear error decreases from 0.25% FS to 0.015% FS and the maximum relative error from 0.38% to 0.022%. After temperature compensation, the temperature coefficient decreased to${0.002}\%\mathrm{{FS}}/{}^{\circ}\mathrm{C}$, which increased by${80}\%$compared to$\pm {0.01}\%$FS/${}^{\circ}\mathrm{C}$before compensation. The dynamic response time is in the${1.7}\sim {2.0}\mathrm{\;{ms}}$range, with a$-3\mathrm{\;{dB}}$bandwidth of${850}\sim {870}\mathrm{\;{Hz}}$. Conclusion The error compensation method proposed in this paper can effectively improve the measurement accuracy of pressure detection systems and provide new ideas for the development of high-precision pressure detection technology.

Objective To prepare cultivated soil standard material for agricultural soil testing process to ensure that the test process is closer to the actual test samples. Methods The candidate samples prepared by drying, crushing, grinding, mixing, packing, using the$F$test uniformity test, examined the long-term stability of the candidate in storage for more than 18 months and short-term stability of simulated transportation conditions, invited 10 long undertake the soil standard material value laboratory cooperation value, using a variety of test methods and the current advanced and reliable test methods, copper and nickel test, the sample with the average as the specified value, the final synthesis uncertainty. Results Developed by soil composition analysis standard material uniformity is good, for 18 months of stability monitoring, through the calculation evaluation for 5 years, the fixed value project comprehensive accurate, reasonable uncertainty assessment, the relative extension of copper uncertainty is${6.5}\%$, the relative uncertainty of total nickel is${6.7}\%$, better than similar concentration of other same grade of soil national standard material. Conclusion The standard material is widely used and can be used for soil composition analysis in agriculture, forestry, environment and geochemistry.

Objective To develop a gas chromatography-mass spectrometry solid-phase extractionfor detectingphthalic acid esters (PAEs) in the serum of patients with thyroid cancer. Four PAEs, namely, dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), and di (2-ethyl) hexyl phthalate (DEHP), were used in experiments. Methods Samples of PAEs were extracted and enhanced using a solid-phase extraction glass column. The column temperature parameters were as follows: the initial temperature was${60}^{\circ}\mathrm{C}$. The temperature was maintained at${60}^{\circ}\mathrm{C}$for 1 min and then increased by${20}^{\circ}\mathrm{C}/\mathrm{{min}}$to${220}^{\circ}\mathrm{C}$. The temperature was then increased by${5}^{\circ}\mathrm{C}/\mathrm{{min}}$to${290}^{\circ}\mathrm{C}$and maintained at${290}^{\circ}\mathrm{C}$for 2 min. The mass spectrometry conditions were as follows: the ionization mode was electron impact, the source energy was${70}\mathrm{{eV}}$, the ion source temperature was${240}^{\circ}\mathrm{C}$, the quadrupole temperature was${150}^{\circ}\mathrm{C}$, and the scanning mode was selective ion monitoring (SIM). These parameters were qualitatively based on the target compound’s retention time and the mass-to-charge ratio (m/z). Quantitative analysis was conductedusing the standard internal method. Results The standard curve regression equations of DMP, DEP, DBP, and DEHP all had excellent linear relationships with correlation coefficients greater than 0.997. The precision of DMP, DEP, DBP, and DEHP was 2.60%, 4.73%, 5.50% and 5.70%, respectively. The average recoveries were in the average range of${94.5}\%$. The detection rate of DBP, DMP, DEP and DEHP were 100%,31.6%,73.46%, and${100}\%$, respectively. Conclusion The method is suitable for simultaneous detection of PAEs in human serum, with low organic dose, short extraction time, good recovery and reproducibility, and low detection limit.

In this study, a detection method based on infrared thermal imaging technology is developed for the defect detection problem of agricultural distribution network lines. This method combines advanced laser technology and infrared thermal imaging analysis to effectively improve the accuracy and efficiency of detection. The laser device is used to thermal motivate the rural distribution network line from multiple angles, and then the thermal image data is collected through an infrared thermal imager. The in-depth analysis of the thermal images accurately identifies the tiny cracks and other potential defects in the line. Experimental data show that this method can significantly improve the SNR of defect identification and reduce the missed detection rate, and it is an efficient detection technology adapted to meet the needs of modern agricultural power grid. This technology not only optimizes the detection process, but also greatly shortens the detection time, and improves the operation safety and reliability of the rural distribution network.

Objective To establish an ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of the characteristic components of Ophiopogon japonicus and Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma saponins B and Liriope muscari (Decne.) Baily saponins C in Xuanmai Ganju granules. Methods Xuanmai Ganju Granules were extracted by methanol ultrasound and analyzed using OMNI Orca${\mathrm{C}}_{18}$chromatography column (${2.1}\mathrm{\;{mm}}\times {100}\mathrm{\;{mm}},{2.6\mu }\mathrm{m}$). The mobile phase was eluted using a gradient elution of${10}\mathrm{{mmol}}/\mathrm{L}$ammonium acetate solution and methanol. The column temperature was set to${40}^{\circ}\mathrm{C}$, and the injection amount was${1\mu }\mathrm{L}$. The contents of methyl ophiopogon flavanone$\mathrm{A}$and methyl ophiopogon flavanone$\mathrm{B}$in Ophiopogon japonicus, as well as saponin B in Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma and saponin C in Liriope muscari (Decne.) Baily, using an electrospray ion source (ESI), a simultaneous scanning mode of positive and negative ions, and a multi reaction monitoring mode. Results Methyl ophiopogon flavanone A, methyl ophiopogon flavanone B, saponin B from Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma, and saponin C from Liriope muscari (Decne.) Baily have good linear relationships within their respective ranges, with $r$ values greater than 0.996 and recovery rates ranging from 90.6% to 99.8%. Conclusion This method has the advantages of simple and fast pre-treatment, high sensitivity, and accurate and reliable results. It can be used to determine the feeding situation of Ophiopogon japonicus mixed with Liriope spicata (Thunb.) Lour. in commercially available Xuanmai Ganju Granules and provide legal basis for regulatory authorities.

Objective To establish a specific, reliable and accurate quality control method to improve the quality standard of Anwei Granules. Methods The chromatography system, temperature and humidity of different reagents were investigated by silica gel thin layer chromatography (TLC) to optimize the thin layer identification of Coptidis rhizoma, Radix salviae miltiorrhizae and Linderae Radix. The content of total alkaloids in the preparation was determined by UV-Vis. Results The development system of Coptidis rhizoma was determined to be n-butanol-acetic acid-water (7 : 2.5 : 2), the development system of Radix salviae miltiorrhizae was toluene-trichloromethane-ethyl acetate-methanol-formic acid$\left({2 : 3 : 4 :{0.5}: 2}\right)$, and the development system of Linderae Radix was trichloromethane-ethyl acetate-formic acid$\left({4 : 3 :{0.2}}\right)$. The negative results of the three drugs were not interfered. The total alkaloid content was${4.8328}\mathrm{{mg}}/\mathrm{g}$and RSD% was 1.83% by UV-Vis assay. Conclusion In this study, an effective method for improving the quality standard of Anwei Granules was established, which has the characteristics of good repeatability and strong specificity.

Objective This study aims to improve the accuracy and reliability of the determination of active ingredient content in Enteritis Ning Granules by optimizing high-performance liquid chromatography (HPLC) technology, and comprehensively evaluate the uncertainty of the determination results. Methods In this study, the Agilent 1260 HPLC system was used to determine the content of gallic acid active ingredient in Enteritis Ning Granules by optimizing chromatographic conditions such as mobile phase ratio, detection wavelength, column temperature, and solvent. At the same time, establish mathematical models toquantify multiple sources of uncertainty such as instrument errors, operational errors, and sample preparation errors. Results The optimized chromatographic technique significantly improved the sensitivity and accuracy of detecting active ingredients in Enteritis Ning Granules. The content of gallic acid is${27.5}\mathrm{{mg}}$, the chromatographic peak shape is symmetrical, and the separation degree and theoretical plate number have reached the ideallevel. The uncertainty analysis shows that the total uncertainty is small$\left({{U}_{\text{total }}={5.40}\%}\right)$, indicating that the optimized chromatographic technique has high reliability. Conclusion This study successfully optimized the HPLC method for determining the content of Enteritis Ning Granules, and demonstrated the accuracy and reliability of the method through a comprehensive evaluation of uncertainty. This provides a more accurate and reliable analytical method for the quality control of Enteritis Ning Granules, and also offers a new methodology for the modernization analysis of traditional Chinese medicine preparations.

Objective To study the determination of mercury and arsenic content in soil by microwave digestion/atomic fluorescence method. Methods In this study, microwave digestion was used as the pretreatment, by optimizing the digestion temperature, digestion solution dosage and other parameters, digested with aqua regia (a mixture of hydrochloric acid and nitric acid), and the contents of mercury and arsenic in the samples were determined by atomic fluorescence. Results The results showed that the correlation coefficients of the standard curves of mercury and arsenic were 0.9996 and 0.9999, and the detection limits of the method were 0.0011 mg/kg and${0.0096}\mathrm{{mg}}/\mathrm{{kg}}$, respectively. The relative standard deviation of parallel experiments was${1.3}\%\sim {8.7}\%$, all of which were within 10%. The recoveries were 91.2%~117.4% and 86.0%~98.0%, respectively. The measured values of the certified reference materials are all within the theoretical range. Conclusion By optimizing digestion temperature, digestion solution dosage and other parameters, the method is accurate, simple, reliable and has good practicability.

Objective The relationship between fitness and single nucleotide gene polymorphism of Yersinia pestis was investigated. Methods In this paper, the mechanism of GTG in competition between different strains was revealed through continuous in vitro co-incubation competition and in vitro static competition. Results With the increase of passage times, GTG strain quickly became the absolute dominant strain and showed competitive adaptability. Under different stresses (Asp deficiency, weak acid and high salt), GTG and TTG strains showed more remarkable adaptability. In the competitive survival test of macrophages, the ratio of GTG strains to TTG strains did not change significantly. In the intracellular competitive amplification product experiment, mice with GTG: TTG=10: 1, the average dose exceeded the theoretical value during the administration process, resulting in early death of the mice. Conclusion GTG has competitive adaptability among different strains. There was no significant difference between the fitness of GTG and TTG strains in the intracellular competition of macrophages and the competition in mice, but TTG strains showed a fitness advantage in E. coli competition.

The comprehensive scientific research experimental platform of university affiliated hospitals integrates medical treatment, scientific research and teaching, which is the pioneer field of medical research and the core element of scientific and technological innovation system. This paper aims to analyze the current situation and dilemma of safety management of scientific research experimental platform, combined with national policies, take the affiliated hospital of Youjiang Medical University for Nationalities as an example, and introduce the practical experience of safety management of comprehensive scientific research platform in affiliated hospitals of universities. It includes comprehensively strengthening the safety awareness of scientific research personnel, comprehensively improving the safety management system and process, rationally planning the construction layout of scientific research platform, and enabling scientific research platform safety management, etc., providing a referable safety management mode of scientific research platform for affiliated hospitals of universities.

Objective To study the killing effect of glycine combined with iodophor disinfectant on drug-resistant Staphylococcus aureus. Methods Neutralizing agent identification and suspension quantitative killing experiment were used to observe the effect of glycine compound combined with iodophor disinfectant in killing drug-resistant Staphylococcus aureus. Results Iodophor can kill Staphylococcus aureus, with a dilution sterilization efficiency of 63.7%. At the same time, the addition of 4% glycine and a synergistic iodophor disinfectant found a sterilization rate of 79.6%. When killing Staphylococcus aureus in suspension, there was a significant promotion of sterilization effect, which was significantly different from the group with only iodophor$\left({P <{0.01}}\right)$. Conclusion Glycine compounds can significantly enhance the killing effect of iodine on Staphylococcus aureus and increase the sensitivity of bacteria to disinfectants.

In recent years, with the increasing concern of the public regarding drug safety, the detection capabilities of drug inspection systems have been continuously improving. There has been an explosive growth in the number of drug inspection equipment, which poses new challenges for equipment quality management. Currently, there are some issues in the equipment quality management of drug inspection units. This article analyzes the problems existing in the equipment quality management of drug inspection units and proposes corresponding improvement suggestions and measures. The aim is to provide scientific and effective management approaches for the equipment quality management of drug inspection departments, and to collectively strive for improved efficiency in drug inspection equipment quality management.

Due to the strong randomness of fault distribution and low reliability of detection results in zinc oxide lightning arresters, a fault detection method for zinc oxide lightning arresters based on infrared thermal imaging technology is proposed. The FLIR T600 infrared thermal imager with a resolution of${640}\times {480}$pixels is used to capture thermal sensing images of lightning arresters. The fault area is identified through temperature characteristics of the fault location and binary image processing, and the detection output of temperature abnormal areas is obtained. The test results show that the detection results of different areas of the zinc oxide lightning arrester device are completely consistent with the manual detection results, and there are no missed or false detections.

Objective To establish a rapid method for the determination of benzo [a] pyrene in vegetable oil by liquid-liquid extraction-high performance liquid chromatography. Methods The sample was extracted by acetonitrile oscillation, and after centrifugation, the supernatant was taken and passed through${0.22\mu }\mathrm{m}$filter membrane, and injected into high-performance liquid chromatograph for measurement. Results were quantified using matrix external standard method. Results Benzo [a] pyrene had a good correlation in the linear range of${0.5}- {20.0}\mathrm{{\mu g}}/\mathrm{{kg}}$, and the correlation coefficient${r}^{2}$was$\geq {0.9990}$. The recoveries at 0.5,2.0 and${10.0}\mathrm{{\mu g}}/\mathrm{{kg}}$were${87.0}\%\sim {111.2}\%$, and the relative standard deviations were${2.2}\%\sim {14.2}\%$. The limits of detection and quantitation were${0.2\mu }\mathrm{g}/\mathrm{{kg}}$and${0.5\mu }\mathrm{g}/\mathrm{{kg}}$, respectively. Conclusion The method is simple, rapid and accurate, and is suitable for the determination of benzo [a] pyrene in vegetable oil.

Objective To establish a method for the determination of total boron in soil by alkali fusion-inductively coupled plasma emission spectrometry to meet the quality control requirements of the third national soil census. Methods In this experiment, the alkali fusion method was used for sample pretreatment. By optimizing experimental conditions and screening the optimal curve preparation method, three standard substances with different contents were determined by inductively coupled plasma atomic emission spectrometry. Results The linear equation and correlation coefficient of the calibration curve prepared with experimental water are$y ={1171.470x}+ {12.981}\left({{r}^{2}= {1.000}}\right)$. The accuracy test was carried out with national standard substances, and the relative error is 13%~33%. The linear equation and correlation coefficient of the curve prepared with matrix matching solution are$y ={818.529x}+ {10.124}\left({{r}^{2}= {0.999}}\right)$. The accuracy test was carried out with national standard substances, and the relative error is${1.9}\%\sim {7.7}\%$. Conclusion This shows that preparing the calibration curve with matrix matching solution can eliminate the matrix interference caused by alkali fusion and obtain satisfactory results.

The normal module operating temperature (NMOT) of photovoltaic modules plays an important role in analyzing the actual power generation performance of the modules, but it is difficult to accurately measure. In the experimental teaching of heat transfer course, the introduction of photovoltaic module temperature rise cases has expanded the scope of teaching cases, cultivated students' ability to solve engineering problems of photovoltaic module temperature rise modeling using heat transfer mechanisms, and broadened their knowledge of factors affecting photovoltaic power generation. Finally, the theoretical calculation method for the temperature rise characteristic model of photovoltaic modules was analyzed, and experiments were designed to verify the accuracy of the model. The validated model was used to calculate the NMOT of photovoltaic modules

In EMC (Electromagnetic Compatibility) testing, radiation testing occupies an important position and has significant influence. Based on the requirements of relevant job positions in enterprises for EMC test engineers, we conducted two rounds of lectures to sort out, research, and think and analyze, and developed a small and economical radiation disturbance test training platform. On the one hand, it avoids the high cost of dark room construction and the shortage of space, and on the other hand, it fills the gap in radiation interference testing teaching and helps students prepare for entering enterprises, helping them better adapt to the needs of enterprise job positions. This paper aims to develop a small and economical radiation interference test training platform, mainly explaining the development process from two aspects of key equipment and system design and construction to meet the needs of teaching and research, and also has guiding value for actual engineering applications.

This paper aims to explore the safety management construction of engineering laboratories in higher education, focusing on two aspects: safety management in engineering laboratories and the efficiency of laboratory equipment use. Specifically, safety management construction includes equipment usage safety, safety protocols for high-altitude operations in engineering testing venues, and management of external personnel. A scientifically sound management system can effectively reduce equipment usage risks and ensure testing safety. For high-altitude operations, which are common high-risk activities, strict safety regulations and emergency plans must be established to ensure the safety of operators. Additionally, managing the access of external personnel should not be neglected; it is essential to clarify their access rights and safety responsibilities to enhance the overall safety measures of the laboratory. By strengthening these three aspects of management and improving equipment usage efficiency while reducing idle and loss rates, we can not only effectively enhance the safety of the laboratory but also promote the high-quality development of engineering education.

Objective To solve the zero drift problem of Coriolis mass flowmeter. Methods The zero calibration method based on Prolink industrial control software was used to measure and zero the CMF mass flowmeter, and data on indication error and repeatability were obtained. Results Before zeroing, the reading error of the Coriolis mass flowmeter was measured to be -0.85%, and it was calculated that there was zero drift. After zero calibration, the indication error of the flowmeter is -0.12%, and the measurement performance meets the requirements of the accuracy level. Conclusion After using Prolink industrial control software to adjust the zero value of the flowmeter, the influence of zero drift on the phase difference offset of the flowmeter can be eliminated, thereby improving the measurement accuracy of the flowmeter.

Objective To establish a titration method for evaluating the uncertainty in the determination of thiomersal content in adsorbed diphtheria, tetanus, and acellular pertussis combined vaccine, and to identify the sources of significant uncertainty, improve laboratory detection ability. Methods The titration method was established according to the first method of the General Rules 3115 in the 2020 edition of the Pharmacopoeia of the People’s Republic of China, a mathematical model was established, and the sources of uncertainty in the detection process were analyzed to synthesize the standard uncertainty and extended uncertainty. Results In the determination of thiomersal content, all influencing factors have a certain effect. After type A evaluation and type B evaluation, the extended uncertainty${U}_{\mathrm{{rel}}}= {4.72}\%\left({K = 2}\right)$was obtained. Conclusion After evaluating the uncertainty introduced by each factor, it was found that the factors introducing significant uncertainty are mainly the titration process of the test sample. It is suggested that the titration process in low-temperature and other conditions be considered in subsequent method improvements.

This article aims to study common technical issues in the monitoring of building energy-saving materials and propose quality monitoring and management measures. In the process of building energy-saving buildings, energy-saving materials play an indispensable core role, and their performance and quality directly determine the level of building energy-saving efficiency. The quality inspection of energy-saving building materials is a key link in evaluating the qualification of building energy-saving projects and needs to be taken seriously. On the basis of analyzing the importance of building energy-saving material testing, this article explores the common problems in current energy-saving material quality testing, and proposes targeted improvement plans and response strategies based on practical experience and relevant national regulations.

With the development of modern medicine, fluorescence immunochromatography plays an important role in the detection of pathogenic microorganisms because of its rapid, simple and high sensitivity. In this paper, the principle and application process of the technique are systematically described, including sample pretreatment, preparation of fluorescently labeled antibodies, assembly of immunochromatographic apparatus, detection reaction and result interpretation. The application examples of the technology in the detection of bacteria, viruses and fungi were introduced, and the advantages and challenges of the technology were analyzed. The results show that fluorescence immunochromatography provides an effective tool for rapid identification and monitoring of pathogenic microorganisms, and has a wide application prospect in clinical diagnosis and public health.

With the increasing prosperity of the toy market, children are exposed to a wide variety of toys, but the hidden harmful chemical substances can not be ignored. The purpose of this paper is to focus on the analysis of common toxic and harmful substances in the toy manufacturing process, through technological innovation and application, the improvement of standards and regulations and other measures to ensure that the toy market products are safe and harmless, protect children from harmful substances, provide scientific basis and practical guidance for government regulators, manufacturers and consumers, and promote the healthy development of the toy industry. Protect children's right to healthy growth.

In recent years, laboratory accidents in colleges and universities have occurred from time to time, and controlling laboratory safety has been the focus of university managers in recent years. In the practice of whole-process laboratory management, further exploration is needed. Combined with the current situation of the laboratory where the management of temporary hazards is difficult, the equipment is incompatible with the laboratory environment, and the improvement of students' safety literacy is limited, it is proposed to integrate the concept of dynamic management into the whole process management process, and realize the whole process dynamic safety management of the four elements of personnel, reagents, equipment and environment with the help of the school laboratory safety intelligent monitoring and control system, which can effectively improve the whole process management process of the laboratory.

Laboratory safety accidents have occurred from time to time, and their harm and impact are far-reaching. In view of the characteristics of agricultural laboratory, such as high density and strong mobility of personnel, diversified and complex instruments and equipment, large types and quantities of chemicals, many kinds of wastes and complex components, etc., combined with the problems in safety management, countermeasures are proposed in terms of funding investment, management system, daily management level, safety inspection, safety education, providing a solid foundation for building a safer and more harmonious laboratory environment and reliable guarantee for the school to achieve the construction of a "Double First Class" university.