Full
Outline
To investigate the effect and mechanism of water extract of Buyang Huanwu Decoction (补阳还五汤, BHD) on H2O2-induced apoptosis in PC12 cells based on the JNK/c-Jun signaling pathway.
An oxidative stress cell model was established by treating PC12 cells with H2O2 at an optimal concentration of 200 μmol/L. The experiment was divided into a normal group, a model group, low-, middle-and high-dose BHD groups, and an SP600125 group. PC12 cells were treated with SP600125 and different concentrations of BHD medium. The cell proliferation was detected using a real-time cell analysis (RTCA) system and the CCK-8 method. Intracellular reactive oxygen species (ROS) levels and apoptosis were measured by immunofluorescence and flow cytometry. The protein expression levels of Bax, Caspase-3, Bcl-2, c-Jun, p-c-Jun, p-JNK and JNK were detected by Western blotting.
Compared with the normal group, the model group showed significantly increased ROS levels, apoptosis rate, protein expressions of Bax and Caspase-3 and ratios of p-JNK/JNK and p-c-Jun/c-Jun, while the expression of Bcl-2 protein was significantly decreased (P<0.01). Compared with the model group, high-dose BHD intervention significantly reduced ROS levels, apoptosis rate, protein expressions of Bax and Caspase-3 and ratios of p-JNK/JNK and p-c-Jun/c-Jun (P<0.01), while significantly increasing the expression of Bcl-2 protein (P<0.01).
The water extract of Buyang Huanwu Decoction can inhibit oxidative stress-mediated apoptosis in PC12 cells through the JNK/c-Jun signaling pathway, revealing a potential mechanism by which Buyang Huanwu Decoction may improve refractory erectile dysfunction by promoting nerve injury repair.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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