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Outline
To observe the role of Cyt-c/Apaf-1/Caspase-9/3 apoptosis signaling pathway in lupus nephritis (LN) and explore the mechanism of Sanqi Injection (三七注射液) in the treatment of LN.
Fifteen female MRL-faslpr mice were randomly divided into model group, Apaf-1 inhibitor group and Sanqi Injection group, with 5 mice in each group. Five C57BL/6 mice of the same age were set as the normal control group. The expressions apoptosome and related proteins in renal tissue were detected by immunofluorescence and western blot.
In the model group, the renal structure of the mice was severely damaged, and the infiltration of inflammatory cells was significant. In the Apaf-1 inhibitor group and the Sanqi Injection group, the renal injury was mitigated and the infiltration of inflammatory cells was reduced. Immunofluorescence analysis of kidney tissue apoptosome revealed that the expression levels of Cyt-c, Apaf-1 and Caspase-9 in the model group were higher than those in the normal group (P<0.01). The expression levels of Cyt-c, Apaf-1 and Cleaved-Caspase-9 in the Apaf-1 inhibitor group and Sanqi Injection group were lower than those in the model group (P<0.01). The protein expression levels of Cyt-c, Apaf-1, Cleaved-Caspase-9 and the expression levels of the apoptotic proteins Cleaved-Caspase-3 and Bax in the model group were higher than those in the normal group (P<0.01), while the expression level of Bcl-2 protein was lower than that in the normal group (P<0.01). The protein expression levels of Cyt-c, Apaf-1, Cleaved-Caspase-9, and the expression levels of apoptotic proteins Cleaved-Caspase-3 and Bax in the Apaf-1 inhibitor group and Sanqi Injection group were lower than those in the model group (P<0.01), while the expression level of Bcl-2 protein was higher than that in the model group (P<0.01).
Once the Cyt-c/Apaf-1/Caspase-9/3 apoptotic signaling pathway is activated, it promotes cell apoptosis and exacerbates the disease severity of LN. Sanqi Injection may alleviate LN by inhibiting the Cyt-c/Apaf-1/Caspase-9/3 apoptotic signaling pathway and reducing apoptosis of renal tissue cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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