To investigate the interventional effect of Shengdihuang（Rehmanniae Radix）on kidney damage in metabolic syndrome mice.

Male ICR mice were fed with 30% fructose solution for 12 weeks to establish a mouse model of metabolic syndrome. After 12 weeks ofmodeling，themice were orally administered with extract of Shengdihuang（Rehmanniae Radix）（2、4 g/kg）and allopurinol（0.005 g/kg，positive control）for 12 weeks，and 30% fructose was continued during this period. The levels of body weight，insulin sensitivity，serum insulin，triglyceride（TG），total cholesterol（TC），high density lipoprotein cholesterin（HDL-C），low density lipoprotein cholesterin（LDL-C），uric acid，creatinine，urinary uric acid and albuminuria were measured. H & E staining was used to observed renal tissue histology，and immunohistochemistry staining was used to observe the expressions of glucose transporter 9（GLUT9）and urate anion transporter 1（URAT1）in kidney tissue. The levels of inflammatory cytokines interleukin-1β（IL-1β），interleukin-18（IL-18），interleukin-6（IL-6）and tumor necrosis factor-alpha（TNF-α）in the renal tissue were detected. Immun of luorescence stainingwas used to observe the expression of nuclear factorκB（NF-κB）in kidney tissue.

Shengdihuang（Rehmanniae Radix）exhibits potential in reducing body weight，improving insulin resistance and blood lipid abnormalities，lowering serum uric acid levels，promoting uric acid excretion by down-regulating the expressions of GLUT9 and URAT1，decreasing the serum levels of creatinine and urinary albumin，ameliorating renal histopathological changes，inhibiting nuclear factor-κB（NF-κB）activation，and the attenuating levels of renal inflammatory cytokines IL-1β，IL-18，IL-6 and TNF-αin fructose-fed mice.

Shengdihuang（Rehmanniae Radix）can promote uric acid excretion by regulating the expressions of urate transport proteins GLUT9 and URAT1，thereby alleviating kidney inflammation in fructose-fed mice.

To investigate the effects of Shenqi Fuzheng Injection（参芪扶正注射液）on the proliferation，invasion and migration of cisplatin-resistant A549/DDP cells of human lung adenocarcinoma and the intervention on the epithelial-mesenchymal transition（EMT）and drug resistance regulated by Keratin（KRT19）.

A549/DDP cells were random ly divided into 4 groups（control group and medication group）. The medication groups were given cisplatin，Shenqi Fuzheng Injection and Shenqi Fuzheng Injection+cisplatin intervention respectively. CCK8 was used to selected the optimal intervention concentration of Shenqi Fuzheng Injection. Trans well was used to observe the cell invasion and migration ability. EdU staining was used to observe the cell proliferation ability. The expressions of KRT19，EMT and resistance-associated proteins（E-Cadherin，N-cadherin，Vimentin，ABCC1，ABCG2）were detected by Western Blot.

Compared with those in the control group，the cell invasion，migration and proliferation abilities in themedication intervention group were significantly reduced. Among them，the cell invasion，migration and proliferation abilities in the Shenqi Fuzheng Injection+cisplatin group were further decreased than those in the cisplatin group（P<0.05）. Compared with those of the cisplatin group，the protein expression levels of KRT19，N-cadherin，Vimentin，ABCC1 and ABCG2 in the Shenqi Fuzheng Injection+cisplatin group were significantly down-regulated（P<0.05），and E-Cadherin protein expression was significantly up-regulated（P<0.05）. The differences were statistically significant.

Shenqi Fuzheng Injection can inhibit the proliferation，invasion and migration of A549/DDP cells，and increase the sensitivity of cisplatin，which may be related to the intervention of EMT and drug resistance regulated by KRT19.

To investigate the role of Zuogui Pill（左归丸）in regulating Janus kinase 2（JAK2）signal transducer and activator of transcription 3（STAT3）in the retinal development of prenatal stress（PS）rats.

Thirty-two pregnant SD rats were random ly divided into blank control group，model group，Zuogui Pill group（18.9 g·kg^-1）and vitamin E group（1.44 mg·kg^-1），with 8 rats in each group. The other groups were established PSmodel by chronic unpredictable mild stress（CUMS）. The PSmodel was evaluated by behavioral tests，and the serum levels of glucocorticoid（GC）and thyroxine-4（T4）were measured by enzyme-linked immunosorbent assay（ELISA）. The morphological changes of the retina of the hematoxylineosin（HE）staining at18，21 and 21 days after birth were observed，and the expression of epidermal growth factor receptor（EGFR）protein in the retina of the offsprings was detected by immunofluorescence. The protein expressions of EGFR and JAK2/STAT3 pathway were detected by Western blotting.

Compared with those in the model group，the preference of sugar water，the ability of exercise exploration and the contentof serum T4 were decreased，while the level of GCwas increased in the model group（P<0.05）. In Zuogui Pill group and vitamin E group，the glucose preference，exercise exploring ability and T4 levels of pregnantmice were increased，while the GC levelwas decreased（P<0.05）compared with those of the model group. The model group showed delayed retinal differentiation in E18 and P0，severe retinal pathological damage in P21 and weakened EGFR positive signal. The expression levels of p-EGFR/EGFR，p-JAK2/JAK2 and p-STAT3/STAT3 in the retina were significantly decreased（P<0.05）. Zuogui Pillgroup and vitamin E group can improve the above abnormal indexes in different degrees.

Thematernal intervention of Zuogui Pill can improve the effect of PS on the retinal development of offspring rats through JAK2-STAT3.

To explore the effect of Roucongrong（Cistanches Herba）on nerve cell injury in Parkinson＇s disease（PD）rats and its related mechanism.

Sprague-dawley（SD）male rats were random ly divided into three groups（10 rats in each group）：control group，modelgroup and Roucongrong（Cistanches Herba）group. The rats in the modelgroup and the Roucongrong（Cistanches Herba）group were intracranially injected with 6-hydroxydopamine（6-OHDA）. The rats in the Roucongrong（Cistanches Herba）group were given Roucongrong（Cistanches Herba）solution（0.216 g/mL），and those in the blank group and model group were given the normal saline irrigation［1 mL（100 g·d）］for two weeks. Themorphology and ultrastructure of nerve cells in hippocampuswere observed by hematoxylin-eosin staining and transmission electronmicroscopy. Themorphology of Nishi bodies in the Hemimani region was observed by Nishi＇s staining. The levels ofmalondialdehyde（MDA）and superoxide dismutase（SOD）were detected by thiobarbituric acidmethod and nitroblue tetrazolium method. The changes of Kelchlike ECH-related protein 1（Keap 1），nuclear factor E2-related factor2（Nrf2）and heme cyclooxygenase 1（HO-1）proteins were analyzed byWestern blot.

After the treatment of Roucongrong（Cistanches Herba），the hippocampal tissue structure of PD rats was improved，themorphology of neurons was improved，the nuclear pyretosis was relieved，the content of antioxidant enzyme SOD in hippocampuswas gradually increased，while the contentof oxidative stress productMDA was gradually decreased，and the expression of Keap 1 protein in hippocampus was decreased. The protein expressions of Nrf 2 and HO-1 increased.

Roucongrong（Cistanches Herba）can improve the damage of hippocampus neurons，and reduce the oxidative stress response in hippocampus of PD rats. The effect of Roucongrong（Cistanches Herba）in the treatmentof PD rats is closely related to the regulation of Keap-1/Nrf2/HO-1 signaling pathway.

To explore the clinical efficacy of Modified Ditan Decoction（涤痰汤化裁方）in the treatment of chronic obstructive pulmonary disease（COPD）complicated with pulmonary infection and its effect on pro-inflammatory cytokines interleukin-6（IL-6），interferon-γ（IFN-γ）and tumor necrosis factor-α（TNF-α）in serum.

One hundred and twenty-four patients with chronic obstructive pulmonary disease and lung infection in the hospital from January 2022 to June 2023 were randomly divided into study group（62 cases）and control group（62 cases）. The control group was treated with conventional Western medicine. Based on this，the study group received Modified Ditan Decoction. Then the clinical efficacy，TCM syndrome scores，blood oxygen partial pressure（PaO₂），blood oxygen saturation（SaO₂），arterial blood carbon dioxide partial pressure（PaCO₂），forced expiratory volume in the first second（FEV1），maximal voluntary ventilation（MVV），forced vital capacity（FVC），the ratio of forced expiratory volume to forced vital capacity in the first second（FEV1/FEV2），interleukin-6（IL-6），interferon-γ（IFN-γ）and tumor necrosis factor-α（TNF-α）were compared between two groups.

No statistical difference was reported between two groups in terms of baseline pulmonary function parameters，blood gas index，TCM syndrome scores，or the levels of serum IL-6，IFN-γ and TNF-α（all P>0.05）. The total efficacy rate was 96.77% in study group，which was higher than 87.10% in control group（P<0.05）. The study group had lower TCM syndrome scores than the control group after treatment（P<0.05）. After treatment，the blood gas indexes of the two groups，such as PaO₂ and SaO₂ increased and PaCO₂ decreased，and the improvement in the study group was more obvious（P<0.05）. After treatment，lung function indexes such as FEV1，MVV，FVC and FEV1/FVC in both groups increased，and the improvement in the study group was more obvious（P<0.05）. After treatment，the serum levels of IL-6，IFN-γ and TNF-α in the two groups decreased，and the improvement in the study group was more obvious，with statistical significance（P<0.05）.

Application of Modified Ditan Decoction in the treatment of chronic obstructive pulmonary disease complicated with pulmonary infection can effectively ameliorate the pulmonary function，alleviate the clinical symptoms，and reduce the levels of IL-6，IFN-γ and TNF-αin serum.

To observe the clinical effect of Fupi Yishen Recipe（扶脾益肾方）combined with manual root surface leveling in the treatmentof severe chronic periodontitis（spleen-kidney Yang deficiency type）and its effecton inflammatory factors.

A total of 102 patients with severe chronic periodontitis（spleen-kidney-yang deficiency type）admitted from January 2020 to January 2024 were included in the group study. The patients were divided into the simple operation group and the combined Chinese medicine group by random number tablemethod，with 51 cases in each group. All patients in the simple operation group received manual root planarization treatment. Those in the combined Chinese medicine group were treated with manual root surface leveling and Fupi Yishen Recipe. During the treatmentand study，1 patient in the simple operation group fell off，and 2 patients in the combined Chinese medicine group fell off. The clinical efficacy，integral changes of TCM symptoms（main symptoms，secondary symptoms and tongue and pulse）before and after treatment，the changes of periodontal index and concentration of inflammatory factors in gingival crevicular fluid，the changes of masticatory function（bite force and masticatory efficiency）and the adverse reactions of treatmentof two groupswere observed.

The total effective rate of the combined Chinese medicine group was higher than thatof the operation group（P<0.05）. Before treatment，the scores of TCM symptoms（main symptoms，secondary symptoms and tongue and pulse），periodontal indices such as gingival bleeding index（BI），plaque index（PLI）and loss ofattachment（AL），depth of periodontal probe（PD），concentration of inflammatory factors such as tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in gingival crevicular fluid，and chewing function（biting force）of patients in both groups had no significance（all P>0.05）. After treatment，the above indexes were all improved and those in the combined Chinese medicine group were better than those in the simple operation group（all P<0.05）. No serious adverse reactions were observed in the combined Chinese medicine group and the simple operation group（P>0.05）.

Fupi Yishen Recipe combined with manual root surface leveling has significant clinical effect in the treatment of severe chronic periodontitis（spleen-kidney Yang deficiency type），which can effectively reduce the concentration of inflammatory factors in gingival crevicular fluid，improve the clinical symptoms，periodontal index and masticatory function of patients and promote the recovery of patients. The treatment is relatively safe and reliable.

To observe the clinical effectof Ziyin Xiehuo Prescription（滋阴泻火方）on girlswith precocious puberty and simple obesity.

A total of230 girlswith central precocious puberty accompanied by simple obesity admitted from June 2020 to June2023 were included in the study. The patients were divided into control group and observation group by random number table method，with 115 cases in each group. The girls in the control group were treated with leuprorelin acetate microspheres，while those in the observation group were treated with leuprorelin acetate microspheres combined with Ziyin Xiehuo Prescription. The treatment lasted for 6months. During the treatment，5 cases fell off in the observation group and 4 cases fell off in the controlgroup. The data of allgroupswas compared，including the efficacy，traditional Chinese medicine syndrome score before treatment and 6 months after treatment，the levels of serum estradiol（E2），luteinizing hormone（LH）and follicle-stimulating hormone（FSH），uterine length and ovarian volume，breast stage，bone age，body mass index（BMI）and adverse reactions.

The total effective rate of girls in the observation group was higher than that in the control group. Before treatment，the traditional Chinese medicine syndrome scores，the levels of E2，LH and FSH，uterine length，ovarian volume，breast stage，bone age and BMI of all groupswere not significant（P>0.05）. After treatment，the traditional Chinese medicine syndrome scores，the levels of E2，LH and FSH，uterine length，ovarian volume and breast stage of girls in both groupswere decreased（P<0.05）. After treatment，the levels of traditional Chinese medicine syndrome scores，the levels of E2，LH and FSH in the observation group were lower than those in the control group，the values of uterine length，ovarian volume，breast stage and bone age were lower than those in the control group，and BMI in the observation group was lower than that in the control group. The adverse reaction rate of girls in observation group and controlgroup was low（P>0.05）.

In the treatment of central precocious puberty with simple obesity girls，Ziyin Xihuo Prescription can regulate the levels of sex hormones，delay the development of breast，uterus and ovary，do not aggravate the trend of obesity，and inhibit the growth of bone age to a certain extent.

To explore the effect of Xiagu Xiaoying Prescription（夏菇消瘿方）combined with Chinese medicine on benign simple thyroid nodules.

A total of102 patientswith benign simple thyroid nodules admitted from January 2022 to June 2023 were included and divided into control group and traditional Chinese medicine group by random number table method，with 51 cases in each group. The patients in the control group were treated orally with levothyroxine sodium tablets，and those in the traditional Chinese medicine（TCM）group were treated with Xiagu Xiaoying Prescription and external application of Chinese medicine for 8 weeks on the basis of medication in the control group. During the treatment，4 cases fell off in control group and 3 cases fell off in TCM group. The data of each group was observed，including the clinical therapeutic effect，TCM symptom integral（palpable lump in front of the neck，discomfort in the neck，difficulty breathing，foreign body sensation in the throat，soft and painless mass，emotional depression）before and after treatment，thyroid volume and thyroid nodule diameter change before and after treatment，the levels of thyroid stimulating hormones［thyroid stimulating hormone（TSH），free thyroxine（FT4），free triiodothyronine（FT3）］and other thyroid function indexes and adverse reactions.

The total effective rate of the TCM group（95.83%）was obvious higher than that of the control group（82.98%）（P<0.05）. Before treatment，TCM syndrome scores（palpable lump in front of the neck，discomfort in the neck，difficulty breathing，foreign body sensation in the throat，soft and painless mass，emotional depression），thyroid volume and maximum diameter of thyroid nodule were not significant between two groups（P<0.05）. After treatment，TCM syndrome scores（palpable lump in front of the neck，discomfort in the neck，difficulty breathing，foreign body sensation in the throat，soft and painless mass，emotional depression）of patients in tow groups decreased significantly，and thyroid volume and maximum diameter indexes of thyroid nodule decreased significantly. These indicators in the TCM group were all lower than those of the control（P<0.05）. There were no significant differences in thyroid function indexes（TSH，FT4 and FT3）among two groups after treatment（P>0.05）. The total adverse reaction rate of the TCM group（2.08%）was lower than that of the control group（14.89%）（P<0.05）.

Xiagu Xiaoying Prescription combined with Chinese medicine external application in the treatment of benign simple thyroid nodules can significantly relieve the symptoms，significantly reduce the nodules，the recovery is good，and the treatment of adverse reactions are less. The treatment method is safe and reliable，worthy of application.