Full
Outline
To investigate the interventional effect of Shengdihuang（Rehmanniae Radix）on kidney damage in metabolic syndrome mice.
Male ICR mice were fed with 30% fructose solution for 12 weeks to establish a mouse model of metabolic syndrome. After 12 weeks ofmodeling，themice were orally administered with extract of Shengdihuang（Rehmanniae Radix）（2、4 g/kg）and allopurinol（0.005 g/kg，positive control）for 12 weeks，and 30% fructose was continued during this period. The levels of body weight，insulin sensitivity，serum insulin，triglyceride（TG），total cholesterol（TC），high density lipoprotein cholesterin（HDL-C），low density lipoprotein cholesterin（LDL-C），uric acid，creatinine，urinary uric acid and albuminuria were measured. H & E staining was used to observed renal tissue histology，and immunohistochemistry staining was used to observe the expressions of glucose transporter 9（GLUT9）and urate anion transporter 1（URAT1）in kidney tissue. The levels of inflammatory cytokines interleukin-1β（IL-1β），interleukin-18（IL-18），interleukin-6（IL-6）and tumor necrosis factor-alpha（TNF-α）in the renal tissue were detected. Immun of luorescence stainingwas used to observe the expression of nuclear factorκB（NF-κB）in kidney tissue.
Shengdihuang（Rehmanniae Radix）exhibits potential in reducing body weight，improving insulin resistance and blood lipid abnormalities，lowering serum uric acid levels，promoting uric acid excretion by down-regulating the expressions of GLUT9 and URAT1，decreasing the serum levels of creatinine and urinary albumin，ameliorating renal histopathological changes，inhibiting nuclear factor-κB（NF-κB）activation，and the attenuating levels of renal inflammatory cytokines IL-1β，IL-18，IL-6 and TNF-αin fructose-fed mice.
Shengdihuang（Rehmanniae Radix）can promote uric acid excretion by regulating the expressions of urate transport proteins GLUT9 and URAT1，thereby alleviating kidney inflammation in fructose-fed mice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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