Full
Outline
To investigate the effects of Shenqi Fuzheng Injection（参芪扶正注射液）on the proliferation，invasion and migration of cisplatin-resistant A549/DDP cells of human lung adenocarcinoma and the intervention on the epithelial-mesenchymal transition（EMT）and drug resistance regulated by Keratin（KRT19）.
A549/DDP cells were random ly divided into 4 groups（control group and medication group）. The medication groups were given cisplatin，Shenqi Fuzheng Injection and Shenqi Fuzheng Injection+cisplatin intervention respectively. CCK8 was used to selected the optimal intervention concentration of Shenqi Fuzheng Injection. Trans well was used to observe the cell invasion and migration ability. EdU staining was used to observe the cell proliferation ability. The expressions of KRT19，EMT and resistance-associated proteins（E-Cadherin，N-cadherin，Vimentin，ABCC1，ABCG2）were detected by Western Blot.
Compared with those in the control group，the cell invasion，migration and proliferation abilities in themedication intervention group were significantly reduced. Among them，the cell invasion，migration and proliferation abilities in the Shenqi Fuzheng Injection+cisplatin group were further decreased than those in the cisplatin group（P<0.05）. Compared with those of the cisplatin group，the protein expression levels of KRT19，N-cadherin，Vimentin，ABCC1 and ABCG2 in the Shenqi Fuzheng Injection+cisplatin group were significantly down-regulated（P<0.05），and E-Cadherin protein expression was significantly up-regulated（P<0.05）. The differences were statistically significant.
Shenqi Fuzheng Injection can inhibit the proliferation，invasion and migration of A549/DDP cells，and increase the sensitivity of cisplatin，which may be related to the intervention of EMT and drug resistance regulated by KRT19.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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