Full
Outline
To investigate the role of Zuogui Pill（左归丸）in regulating Janus kinase 2（JAK2）signal transducer and activator of transcription 3（STAT3）in the retinal development of prenatal stress（PS）rats.
Thirty-two pregnant SD rats were random ly divided into blank control group，model group，Zuogui Pill group（18.9 g·kg-1）and vitamin E group（1.44 mg·kg-1），with 8 rats in each group. The other groups were established PSmodel by chronic unpredictable mild stress（CUMS）. The PSmodel was evaluated by behavioral tests，and the serum levels of glucocorticoid（GC）and thyroxine-4（T4）were measured by enzyme-linked immunosorbent assay（ELISA）. The morphological changes of the retina of the hematoxylineosin（HE）staining at18，21 and 21 days after birth were observed，and the expression of epidermal growth factor receptor（EGFR）protein in the retina of the offsprings was detected by immunofluorescence. The protein expressions of EGFR and JAK2/STAT3 pathway were detected by Western blotting.
Compared with those in the model group，the preference of sugar water，the ability of exercise exploration and the contentof serum T4 were decreased，while the level of GCwas increased in the model group（P<0.05）. In Zuogui Pill group and vitamin E group，the glucose preference，exercise exploring ability and T4 levels of pregnantmice were increased，while the GC levelwas decreased（P<0.05）compared with those of the model group. The model group showed delayed retinal differentiation in E18 and P0，severe retinal pathological damage in P21 and weakened EGFR positive signal. The expression levels of p-EGFR/EGFR，p-JAK2/JAK2 and p-STAT3/STAT3 in the retina were significantly decreased（P<0.05）. Zuogui Pillgroup and vitamin E group can improve the above abnormal indexes in different degrees.
Thematernal intervention of Zuogui Pill can improve the effect of PS on the retinal development of offspring rats through JAK2-STAT3.
37
15
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
