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Outline
To explore the effect of Roucongrong（Cistanches Herba）on nerve cell injury in Parkinson＇s disease（PD）rats and its related mechanism.
Sprague-dawley（SD）male rats were random ly divided into three groups（10 rats in each group）：control group，modelgroup and Roucongrong（Cistanches Herba）group. The rats in the modelgroup and the Roucongrong（Cistanches Herba）group were intracranially injected with 6-hydroxydopamine（6-OHDA）. The rats in the Roucongrong（Cistanches Herba）group were given Roucongrong（Cistanches Herba）solution（0.216 g/mL），and those in the blank group and model group were given the normal saline irrigation［1 mL（100 g·d）］for two weeks. Themorphology and ultrastructure of nerve cells in hippocampuswere observed by hematoxylin-eosin staining and transmission electronmicroscopy. Themorphology of Nishi bodies in the Hemimani region was observed by Nishi＇s staining. The levels ofmalondialdehyde（MDA）and superoxide dismutase（SOD）were detected by thiobarbituric acidmethod and nitroblue tetrazolium method. The changes of Kelchlike ECH-related protein 1（Keap 1），nuclear factor E2-related factor2（Nrf2）and heme cyclooxygenase 1（HO-1）proteins were analyzed byWestern blot.
After the treatment of Roucongrong（Cistanches Herba），the hippocampal tissue structure of PD rats was improved，themorphology of neurons was improved，the nuclear pyretosis was relieved，the content of antioxidant enzyme SOD in hippocampuswas gradually increased，while the contentof oxidative stress productMDA was gradually decreased，and the expression of Keap 1 protein in hippocampus was decreased. The protein expressions of Nrf 2 and HO-1 increased.
Roucongrong（Cistanches Herba）can improve the damage of hippocampus neurons，and reduce the oxidative stress response in hippocampus of PD rats. The effect of Roucongrong（Cistanches Herba）in the treatmentof PD rats is closely related to the regulation of Keap-1/Nrf2/HO-1 signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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