To discuss the clinical effect and safety observation of Suhuang Zhike Capsule（苏黄止咳胶囊）in treating post-thyroidectomy cough.

Two hundred and three patients with post-thyroidectomy cough admitted from January 2021 to September 2023 were included，and assigned into two groups using random number table methods. The control group（101 cases）was given ambroxol aerosol inhalation，while the observation group（102 cases）was given ambroxol aerosol inhalation and Suhuang Zhike Capsule. The treatment in both groups lasted for 7 days. During treatment，2 cases in the observation group and 0 case in the control group were dropped out. Then the clinical efficacy，postoperative cough relief time，postoperative cough disappearance time，daytime cough symptom，nighttime cough symptom，Leicester Cough Questionnaire in Mandarin Chinese（LCQMC），self-rating anxiety scale（SAS），self-rating depression scale（SDS），immunoglobulin-related indexes，Karnofsky performance status scale（KPS）and adverse reactions were compared between two groups.

The observation group reported a higher clinical efficacy rate than the control group［97.00%（97/100）vs 90.10%（91/101），P<0.05］.the observation group showed decreased time to the relief and disappearance of postoperative cough，and had lower daytime and nighttime cough symptom scores compared to the control group（P<0.05）. After treatment，an increase in LCQ-MC score，immunoglobulin-related indexes and KPSscore，as well as a reduction in SASand SDSwere observed in all patients，and both changes were more notable in the observation group than those in the control group（P<0.05）. Both groups yielded a low adverse reaction rate，with no statistical difference（P>0.05）.

Application of Suhuang Zhike Capsule in treating post-thyroidectomy cough can effectively improve immune function and quality of life，which has little adverse reaction and good safety.

To explore the risk factors of elderly patients with coronary heart disease（CHD）and weakness and the effect of using traditional Chinese medicine Gancao Xiexin Decoction（甘草泻心汤）combined with Modified LI Ke’s Shensiwei（李可肾四味加减）.

A total of 120 elderly CHD patients complicated with weakness who received the treatment in the hospital from February 2020 to February 2021 were as the weakness group，and another 120 elderly CHD patients without weakness were as the non weakness group to analyze the risk factors that caused weakness. The weakness group was randomly divided into a combination group and a Western medicine group，with 60 cases in each. The Western medicine group received conventional Western medicine treatment，while the combination group received additional treatment with Gancao Xiexin Decoction combined with Modified LI Ke’s Shensiwei on the basis of the Western medicine group. The treatment courses were both 12 weeks，and the differences in treatment effects between the two groups were compared.

（1）According to Logistic regression model analysis，decreased body mass index（BMI），complicated with acute coronary syndrome，increased Mini Nutritional Assessment Short Form（MNA-SF）score and Charlson comorbidity index were independent risk factors for weakness in elderly CHD patients（P<0.05）. Increased Charlson comorbidity index was an independent risk factor for weakness in elderly CHD patients（P<0.05）. The elevated levels of albumin（ALB）and total protein（TP）can effectively reduce the risk of weakness in elderly CHD patients and were protective factors（P<0.05）.（2）Before treatment，there was no significant difference in serum hemoglobin（Hb），ALB，TP or prealbumin（PA）between the Western medicine group and the combination group（P>0.05）. After treatment，the serum levels of Hb，ALB，TP and PA of the two groups were significantly increased compared to those before treatment（P<0.05），while the serum levels of Hb，ALBand PA of the combination group were higher than those of the Western medicine group（P<0.05）. Before treatment，the grip strength，the levels of Timed Up and Go Test（TUG）and Functional Sit-to-Stand Test（FTSS）between the western and combination groups were not significant（P>0.05）. After treatment，the grip strength of both groups was significantly improved and the levels of TUG and FTSS were significantly reduced（P<0.05）. The grip strength in the combination group was higher than that in the Western medicine group，and the TUGwas lower than that in the Western medicine group（P<0.05）.

Malnutrition and comorbidities are risk factors for concurrent weakness in elderly CHD patients. On the basis of the basic treatment plan of Western medicine，the addition of traditional Chinese medicine Gancao Xiexin Decoction combined with Modified LI Ke’s Shensiwei can significantly improve the treatment effect.

To study the application effect of oral and external use of Tongguan Xiaozheng Decoction（通管消癥汤）and Huangjin Powder（黄金散）in tubal obstructive infertility and its effect on the levels of inflammatory factors.

From January 2020 to December 2021，120 patients with tubal obstructive infertility after tubal interventional recanalization in the hospital were selected and divided into two groups according to the random number table method，with 60 patients in each group. The patients in the control group were treated with conventional western medicine，enema and external application therapy. The patients in the observation group were treated with Tongguan Xiaozheng Decoction on the basis of the control group. After 3 months of treatment，the clinical efficacy，TCM symptom scores，the levels of inflammatory factors interleukin-6（IL-6），interleukin-8（IL-8）and tumor necrosis factor-α（TNF-α）were compared between the two groups. The tubal patency rate and intrauterine pregnancy rate were observed after 12 months of follow-up.

The total effective rate of the observation group was 95.00%（57/60），which was significantly higher than that of the control group（83.33%，50/60）. The difference between the two groups was statistically significant（P<0.05）. After treatment，the scores of abnormal vaginal discharge，lumbosacral pain，stringy pulse and white tongue coating in the two groups were significantly decreased，and those in the observation group were significantly lower than the control group（P<0.05）. After treatment，the levels of IL-6，IL-8 and TNF-αin the two groups were significantly decreased，and the levels in the observation group were significantly lower than those in the control group（P<0.05）. At 12 months after treatment，the tubal patency rate and intrauterine pregnancy rate in the observation group were 91.67%（55/60）and 33.33%（20/60），and those in the control group were 81.67%（49/60）and 23.33%（14/60）. There was no significant difference between the two groups（P>0.05）.

The application effect of oral and external use of Tongguan Xiaozheng Decoction and Huangjin Powder in tubal obstructive infertility is satisfactory. It can reduce the levels of serum inflammatory factors. It is an effective treatment plan and worthy of application.

To evaluate the effect of kidney-tonifying method and ibandronate based on lung-kidney-bone axis in the treatment of lung cancer bone metastasis and the monitoring value of CT for prognosis.

A total of 92 patients with lung cancer bone metastasis were included from September 2020 to September 2023. All patients were obtained from the hospital and it was a retrospective analysis. All patients were divided into group A（46 cases，treated with ibandronic acid）and group B（46 patients，treated with kidney-tonifying method based on lung-kidney-bone axis combined with ibandronate）.TCM syndrome scores，KPSscores，serum tumor marker levels，levels of serotonin（5-HT），prostaglandin E2（PGE2）and endothelin-1（ET-1），and the CT values of osteolytic，osteogenic and mixed bone metastasis before and after treatment were compared between two groups.

After treatment，the scores of both groups decreased compared to that before treatment，and the score of group B decreased compared to that of group A.KPSscores increased compared to those pre-treatment，and the KPSscore of group B increased compared with that of group A. The serum levels of glucose antigen 125（CA125），glucose antigen 199（CA199）and cytokeratin fragment 19（CYFRA21-1）were all decreased compared to those before treatment，and the above indexes of patients in group B decreased compared with those in group A. The serum levels of 5-HT，PGE2 and endothelin-1 were all decreased compared to those before treatment，and the above indexes of patients in group B decreased compared with those in group A. The CT value of bone metastases of osteolytic and mixed lung cancer gradually increased at 3 and 6 months after treatment. Moreover，the CT value of different types of lung cancer increased at 6 months compared with that at 3 months after treatment（all P<0.05）.

The combination of kidney-tonifying method and ibandronate based on the lungkidney-bone axis in the treatment of lung cancer bone metastasis patients can help to alleviate the clinical symptoms，improve the physical function，reduce the serum tumor marker levels and have significant analgesic effects. At the same time，MSCT examination can be used to evaluate the treatment effect of lung cancer bone metastasis patients.

To study the effect of Jiedu Sangen Decoction（解毒三根汤）on CT-26 cells in colon cancer.

Ninety healthy and clean BALB/c mice were randomly divided into control group，model group and Jiedu Sangen Decoction group. The colon cancer mouse models in all groups except for the control group were established. The model group received normal saline by gavage，and the Jiedu Sangen Decoction group received Jiedu Sangen Decoction by gavage. After 1 week，the serum was obtained from each group through eyeball. The serum of mice in each group was co-cultured with CT-26 cells，the cell proliferation was detected by MTT method，the cell migration was detected by Transwell method，the cell invasion was detected by invasion test，and the protein expressions of Bax and bcl-2 were detected by Western blot.

After 24 h，48 h and 72 h of cell cultured，the light absorption values of the Jiedu Sangen Decoction group were 0.198±0.068，0.321±0.103 and 0.462±0.106，which were significantly lower than those of the control group and the model group（P<0.05）. The numbers of migrating cells and invading cells in the Jiedu Sangen Decoction group were（122.03±10.48）/HPand（32.10±5.15）/HP，which were significantly lower than those in the control group and the model group（P<0.05）. The relative expression level of Bax protein in Jiedu Sangen Decoction group was 0.978±0.106，significantly higher than that in the control serum group and model serum group（P<0.05），while the relative expression level of Bcl-2 protein was 0.354±0.092，significantly lower than that in the control group and model group（P<0.05）.

Jiedu Sangen Decoction can inhibit the proliferation，migration and invasion of colon cancer CT-26 cells，and its mechanism may be related to the regulation of the expressionsof Bax and Bcl-2.

Based on gut microbiota，to explore the compatibility rules of Sugemule-4 Decoction.

Seventy-two SPF grade male SD rats were randomly divided into control group（C），model group（M），Sugemule-4 Decoction group（S-4），lack of Suanzaoren（Ziziphi Spinosae Semen）formula group（QSZR），lack of Baidoukou（Amomum kravanh Pierre ex Gagnep）formula group（QBDK），lack of Xianghanqin（Cuminum cyminum L.）formula group（QXHQ），lack of Biba（Piperis Longi Fructus）formula group（QBB），Suanzaoren（Ziziphi Spinosae Semen）group（SZR），Baidoukou（Amomum kravanh Pierre ex Gagnep）group（BDK），Xianghanqin（Cuminum cyminum L.）group（XHQ），Biba（Piperis Longi Fructus）group（BB），Anshen Bunao Liquid（安神补脑液）group（Y）. An insomnia rat model was established by using chronic unpredictable mild stress stimulation（CUMS）＋parachlorophenylalanine（PCPA）method. After modeling，the corresponding drugs were administered for 7 days at a dose of 1.215 g/kg. Both group Cand group M were given the equal amounts of distilled water，while Group Y received a dose of 1.8 mL/kg. The general state and colon tissue morphology of rats in each group were compared after treatment，as well as the levels of 5-hydroxytryptamine（5-HT），γ-aminobutyric acid（GABA），acetylcholine（AChE），prostaglandin D2（PGD2），substance P（SP），vasoactive intestinal peptide（VIP）and gastrin（GAS）in serum，inducible nitric oxide synthase（INOS）content in colon and hypothalamic tissues，and 16SrDNA analysis of gut microbiota.

After treatment，the circadian rhythm of the M group rats was disrupted or even disappeared，and their mental state deteriorated. The mental state of the other groups improved to varying degrees compared to those before treatment. Colon tissue HE staining showed that each treatment group could improve the pathological changes of the model rats to varying degrees. The serum levels of 5-HT，GABA，AChE，GASand VIP in group M rats were significantly altered（P<0.05），while the levels of INOSin the colon and hypothalamus were significantly increased（P<0.05）. The S-4 group was able to significantly improve these changes（P<0.05）. Baidoukou（Amomum kravanh Pierre ex Gagnep）had the strongest regulatory ability on insomnia related neurotransmitter levels，Xianghanqin（Cuminum cyminum L.）had the strongest regulatory ability on brain gut peptide levels，and Biba（Piperis Longi Fructus）had the strongest regulatory ability on INOSlevels in colon and hypothalamic tissues. The gut microbiota of each group at the level of gut microbiota genera was analyzed，linear discriminant effect analysis（LEfSe）was used to compare multiple groups，and 7 key differential bacterial genera were selected. Through analysis，it was found that each component in the formula can regulate the disorder of gut microbiota to varying degrees. Further exploring the relationship between the gut microbiota genus and the ELISA indicators detected in this article，it was found that the Coriobacteriaceae_UCG_002 showed a significant positive correlation with INOSin hypothalamic tissue，Anaerostipes showed a significant positive correlation with INOSin colon tissue and serum VIP，and Bifidobacterium showed a significant negative correlation with serum GAS.

Sugemule-4 Decoction and its split formula can improve the changes of insomnia related neurotransmitters，brain-gut peptide content and intestinal microbiota in the body of insomnia model rats to varying degrees.

To observe the effect of Guilu Erxian Glue（龟鹿二仙胶，GLE）on the prevention and treatment of postmenopausal osteoporosis（PMO）in ovariectomized rats（OVX），and to explore the biological effects of gut microbiota.

The female SD rats were divided into 6 groups by random number table method，thus as Sham，OVX，vancomycin（KSS），GLE-L，GLE-M and GLE-H groups.After 1 week of modeling，the drug was administrated by gavage，and the sample was collected 8 weeks later.HE staining was used to detect bone structure，bone cell morphology and number.Bone microstructure was detected by microCT.Bone metabolism was detected by ELISA.16Sr DNA was used to analyze the V3-V4 region of the gut microbiota.

The results of HE and microCT showed that different concentrations of GLE effectively increased bone trabecular percentage，restored bone microstructure，up-regulated the contents of serum ALP and PINP and down-regulated serumβ-CTX content（compared with OVX group，P<0.05）.OTU and PCoA analysis showed that GLE-M significantly restored the richness and diversity of intestinal flora and the composition of intestinal flora in OVX rats.At the phylum level，Firmicutes increased significantly in OVX group（compared with that in Sham group，P<0.01）and the flora abundance decreased significantly after GLE-M intervention（compared with that in OVX group，P<0.01）.At the genus level，the dominant bacteria in GLE group were lactobacillus，Klebsiella，Fusobacterium and so on.Eubacterium myxus increased in OVX group（compared with that in Sham group，P<0.01），and its abundance recovered significantly after GLE-M intervention（compared with that in OVX group，P>0.05）.

GLE may prevent postmenopausal osteoporosis by regulating gut microbiota.