Full
Outline
To observe the effect of Guilu Erxian Glue（龟鹿二仙胶，GLE）on the prevention and treatment of postmenopausal osteoporosis（PMO）in ovariectomized rats（OVX），and to explore the biological effects of gut microbiota.
The female SD rats were divided into 6 groups by random number table method，thus as Sham，OVX，vancomycin（KSS），GLE-L，GLE-M and GLE-H groups.After 1 week of modeling，the drug was administrated by gavage，and the sample was collected 8 weeks later.HE staining was used to detect bone structure，bone cell morphology and number.Bone microstructure was detected by microCT.Bone metabolism was detected by ELISA.16Sr DNA was used to analyze the V3-V4 region of the gut microbiota.
The results of HE and microCT showed that different concentrations of GLE effectively increased bone trabecular percentage，restored bone microstructure，up-regulated the contents of serum ALP and PINP and down-regulated serumβ-CTX content（compared with OVX group，P<0.05）.OTU and PCoA analysis showed that GLE-M significantly restored the richness and diversity of intestinal flora and the composition of intestinal flora in OVX rats.At the phylum level，Firmicutes increased significantly in OVX group（compared with that in Sham group，P<0.01）and the flora abundance decreased significantly after GLE-M intervention（compared with that in OVX group，P<0.01）.At the genus level，the dominant bacteria in GLE group were lactobacillus，Klebsiella，Fusobacterium and so on.Eubacterium myxus increased in OVX group（compared with that in Sham group，P<0.01），and its abundance recovered significantly after GLE-M intervention（compared with that in OVX group，P>0.05）.
GLE may prevent postmenopausal osteoporosis by regulating gut microbiota.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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