To observe the effect of clinical use of Lujiaojiao（Cervi Cornus Colla）in the treatment of patients with knee osteoarthritis of wind-cold-damp paralysis and deficiency of liver and kidney syndrome.

Ninety-four patients with knee osteoarthritis attending the Department of Orthopaedics of People＇s Hospital Affiliated to Shandong First Medical University from March 2024 to June 2024 were selected and divided into two groups by simple randomisation method. In the Isogia group, 47 cases（2 cases were dislodged and 45 cases were completed the trail）were treated with Glucosamine Sulfate Capsules（trade name: Isogia）, and in the Lujiaojiao（Cervi Cornus Colla）group, 47 cases（2 cases were dislodged and 45 cases were completed the trail）were treated with Lujiaojiao（Cervi Cornus Colla）combined with Isogia. The efficacy and safety of the two groups were counted, and the joint fluid matrix metalloproteinase-3（MMP-3）, metalloproteinase inhibitor-1（TIMP-1）, numerical rating scale（NRS）score, knee score, ultrasound parameters and serum bone metabolism level were compared between the two groups.

The total effective rate of the Lujiaojiao（Cervi Cornus Colla）group was 91.11%（41/45）. The total effective rate of the Isojia group was 75.56%（34/45）. The total effective rate of the Lujiaojiao（Cervi Cornus Colla）group was higher than that of the Isojia group, and there was a difference（P<0.05）. The comparison of knee joint score, ultrasound detection parameters, NRS score and score of main symptoms and signs of knee osteoarthritis before treatment between the two groups showed no difference（P>0.05）. The knee joint scores were higher in both groups after treatment and the scores were higher in the Deer Bone Glue group than those of the Isojia group（P<0.05）. Knee osteoarthritis major symptoms and signs score, synovial thickness, NRS score and joint fluid depth after treatment in both groups were lower than those before treatment, and the scores were lower in the Lujiaojiao（Cervi Cornus Colla）group than those of the Isojia group（P<0.05）. The comparison of joint fluid MMP-3, TIMP-1 and serum bone metabolism before treatment between the two groups showed no difference（P>0.05）. The levels of joint fluid TIMP-1 and serum osteocalcin（BGP）and bone-specific alkaline phosphatase（BALP）were elevated after treatment in both groups compared with those before treatment, and the levels were higher in the Deer Bone Glue group than those of the Isojia group（P<0.05）. The levels of joint fluid MMP-3, MMP-3/TIMP-1 and serum anti-tartrate acid phosphatase isoform 5b（TRACP-5b）and collagen type I C-terminal peptide（CTX-I）were lower in both groups after treatment than those before treatment, and the levels were lower in the Lujiaojiao（Cervi Cornus Colla）group than those of the Isojia group（P<0.05）. A total of 5 cases of adverse reactions occurred in the Lujiaojiao（Cervi Cornus Colla）group, with an incidence rate of 11.11%（5/45）；4 cases occurred in the Isojia group, with an incidence rate of 8.89%（4/45）. There was no difference in the cumulative incidence of adverse reactions between the two groups（P>0.05）.

Lujiaojiao（Cervi Cornus Colla）can maintain the dynamic balance of MMP-3/TIMP-1 in synovial fluid, improve bone metabolism and enhance the efficacy in the treatment for knee osteoarthritis.

To explore the effect of Cinobufagin Capsule combined with metronomic chemotherapy on tumor microenvironment and chemokine receptor 7（CXCR7）and CXCR4 in patients with non-small cell lung cancer（NSCLC）.

One hundred and twenty-two NSCLC patients admitted to the hospital from January 2022 to October 2023 were selected and randomly assigned into two groups using the random number table method, each with 61 cases. The control group was given 3 cycles of metronomic chemotherapy（pemetrexed + cisplatin）. Based on this, The study group was treated with Cinobufagin Capsule for 9 weeks. After treatment, the clinical efficacy, tumor markers[carcinoembryonic antigen（CEA）, cytokeratin 19 fragment（CYFRA21-1）, carbohydrate antigen 125（CA125）], immune function indicators（CD3⁺, CD4⁺, CD8⁺）, positive expression rates of CXCR7 and CXCR4 and adverse reactions were compared between two groups.

The object response rate and disease control rate were 40.98% and 62.30% in the control group, which were 67.21% and 86.89% in the study group, with statistical difference（P<0.05）. The serum levels of CEA, CYFRA21-1 and CA125 showed a reduction in both groups after treatment, and the three tumor markers were notably lower in the study group than those in the control group（P<0.05）. A decline in CD3⁺, CD4⁺ and CD4⁺/CD8⁺ and an increase in CD8⁺ were detected in both groups after treatment（P<0.05）, and the study group had higher levels of CD3⁺, CD4⁺ and CD4⁺/CD8⁺ and lower CD8⁺ lower than those of the control group（P<0.05）. The positive rates of CXCR7 and CXCR4 showed a decrease in both groups after treatment, and were lower in the study group than those in the control group（P<0.05）. The overall incidence rate of adverse reactions including nausea, vomiting, neutropenia, thrombocytopenia and liver function damage in the study group was lower than that in the control group（P<0.05）.

The application of Cinobufagin Capsule combined with metronomic chemotherapy in NSCLC patients can effectively ameliorate the tumor microenvironment, and reduce the positive rates of CXCR7 and CXCR4, which is worthy of promotion.

This study aims to assess the efficacy of Laonian Gusong Formula（老年骨松方）with calcitriol in treating the elderly patients with osteoporotic intertrochanteric fracture of the femur.

A total of 160 elderly patients with osteoporotic intertrochanteric fractures of the femur were enrolled at the Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine from February 2022 to February 2024. The patients were randomly divided into a treatment group（80 cases） receiving Laonian Gusen Formula combined with calcitriol and a control group（80 cases）receiving calcitriol alone. The treatment lasted for 2 months. The therapeutic efficacy, pain relief[visual analogue scale（VAS）], bone mineral density（BMD）, bone metabolism markers[insulin-like growth factor-1（IGF-1）, growth hormone（GH）, bone gla protein（BGP）], daily living ability[Barthel index（BI）]and complication rates were monitored and evaluated.

The treatment group exhibited a significantly higher efficacy in traditional Chinese medicine syndrome treatment with a total effectiveness rate of 96.25%（77/80）, compared to 77.50%（62/80）in the control group（P<0.05）. Improvements in BI scores from 58.62 to 73.75 and reductions in VAS scores from 7.87 to 2.29 in the treatment group were significantly better than those in the control group（P<0.05）. Furthermore, the bone density significantly increased in the treatment group, with the density measurements at the greater trochanter and femoral neck rising from 0.19 to 0.43 and from 0.43 to 0.49, respectively（P<0.000）. Markers of bone metabolism such as IGF-1, GH and BGP also improved markedly（P<0.05）. Additionally, the incidence of complications including pressure ulcers, infections and nausea was significantly lower in the treatment group at 5.00%（4/80）compared to 27.50%（22/80）in the control group（P<0.05）.

The integration of Laonian Gusong Formula with calcitriol significantly enhances the bone density, alleviates the pain and promotes the functional recovery in the elderly patients with osteoporotic intertrochanteric fractures, proving to be an effective treatment strategy for these conditions.

To investigate the effect of Compound Danshen Dripping Pills（复方丹参滴丸）combined with edaravone and dexborneol concentrated solution on serum amyloid in the patients with acute cerebral infarction.

One hundred and fifty inpatients with acute cerebral infarction in the Neurology Department of Tangshan Gongren Hospital from January 2022 to January 2024 were selected and grouped according to different treatment methods. All participants were given butylphthalide treatment. Based on this, the control group（74 cases）was given edaravone and dexborneol concentrated solution, whereas the observation group（76 cases）added Compound Danshen Dripping Pills combined with edaravone and dexborneol concentrated solution. The treatment in both groups lasted for 10-14 d. Then the neurological function recovery, serum amyloid, glycated serum protein, retinol binding protein 4, hemorheological parameters, clinical efficacy and adverse reactions were compared between two groups.

The observation group reported a lower score on National Institutes of Health Stroke Scale and modified Rankin Scale, and a higher Barthel index compared to the control group（P<0.05）. The levels of serum amyloid, glycated serum protein and retinol binding protein 4 decreased in the observation group compared to those in the control group（P<0.05）. The high and low shear whole blood viscosities, plasma viscosity and hematocrit were all smaller in the observation group than those in the control group（P<0.05）. The observation group denoted a higher overall efficacy rate than the control group（P<0.05）, while no statistical difference was found in adverse reaction rate between two groups（P>0.05）.

The application of Compound Danshen Dripping Pills combined with edaravone and dexborneol concentrated solution in patients with acute cerebral infarction can effectively promote the recovery of neurological function, reduce the levels of serum amyloid, glycated serum protein and retinol binding protein 4, and ameliorate the blood viscosity.

To explore the curative effect of Xiaochaihu Jiangni Decoction（小柴胡降逆汤）assisted by esomeprazole in the treatment of gastroesophageal reflux disease（GERD）.

A total of 70 patients with GERD in the hospital from March 2020 to February 2022 were selected as the research objects, and they were randomly divided into two groups, with 35 cases in each group. Both groups were given the standard treatment plan of esomeprazole. After the treatment, the control group continued to intervene without drugs, and the observation group continued to be treated with Xiaochaihu Jiangni Decoction. The clinical efficacy, clinical symptom score before and after treatment, 24 h pH value of esophagus during the 6 months follow-up, and the serum levels of Leptin and interleukin-8（IL-8）of the two groups were statistically compared. And the recurrence during follow-up was compared.

The total effective rate of the observation group was 91.43%（32/35）higher than that of control group 65.71%（23/35）（P<0.05）. The scores of the clinical symptoms（heartburn, belching, acid reflux, retrosternal pain）of the observation group after treatment were lower than those of the control group, 24 h pH value of esophagus during the 6 months follow-up（24 h regurgitation times, regurgitation times >5 min, the longest regurgitation time and the total time percentage of pH <4）, the serum levels of Leptin and interleukin-8（IL-8）were lower than those of the control group（P<0.05）. There was no significant difference in the recurrence rate between the two groups（P>0.05）.

After esomeprazole treatment, the continuous maintenance treatment of Xiaochaihu Jiangni Decoction can effectively relieve symptoms, regulate the serum levels of Leptin and IL-8 and reduce 24 h pH value of esophagus of the patients with GERD, with significant effects and positive significance in preventing recurrence.

To investigate the clinical effect of Xinyue Capsule（心悦胶囊）combined with radial artery approach in the treatment of coronary artery disease.

In this study, 200 patients with coronary heart disease admitted from January 2022 to January 2024 were divided into two groups by random number table method, and were evenly divided into a single intervention group and a combined treatment group, with 100 cases in each group. The patients in the single intervention group received coronary intervention by radial artery approach. Those in the combined treatment group were treated with Xinyue Capsule combined with radial artery approach for coronary intervention. During the treatment process, 3 patients in the simple intervention group fell off（97 patients remained）, 4 patients in the combined treatment group fell off（96 patients remained）, and the data of patients in each group was compared, including the treatment effect, TCM syndrome effect, hemorheological indexes such as D-dimer and fibrinogen before and after treatment and left ventricular posterior wall thickness（LVPWT）, left ventricular ejection fractions（LVEF）, left ventricular end diastolic diameter（LVEF）, left ventricular end diastolic diameter, changes of cardiac function indexes such as left ventricular end diastolic diameter（LVEDD）, changes of serum N terminal proB type natriuretic peptide（NT-proBNP）, visualanalogue scale（VAS）and EuroQol five dimensions questionnaire（EQ-5D）.

The total effective rate of the combined treatment group was significantly higher than that of the simple intervention group（P<0.05）. After treatment, hemorheology indexes（D-dimer and fibrinogen, etc.）, cardiac function indexes（LVPWT, LVEF, LVEDD）, NT-proBNP level, VAS and EQ-5D scores of the patients in both groups were significantly improved. The theses indexes of the combined treatment group were all better than those of the simple intervention group（P<0.05）. The complication rate of the combined treatment group was lower than that of intervention group（P<0.05）.

The clinical effect of Xinyue Capsule combined with radial artery approach in the treatment of coronary artery intervention was relatively significant. After treatment, the patients＇ cardiac function was improved, the pain was relieved, the quality of life was improved, and the complications were reduced. The treatment is safe and reliable.

To study the clinical efficacy of Mahuang Lianqiao Chixiaodou Decoction（麻黄连翘赤小豆汤）combined with routine treatment of primary nephrotic syndrome（PNS）with damp-heat syndrome.

A total of 120 patients with PNS in the hospital from March 2022 to March 2024 were included and assigned into observation and control group randomly. The control group received conventional treatment. The observation group received Mahuang Lianqiao Chixiaodou Decoction additionally. The clinical efficacy, TCMsyndrome scores, the levels of urea nitrogen（BUN）, creatinine（Scr）, albumin（ALB）, 24 h urine protein, T lymphocyte CD4⁺, CD8⁺, CD4⁺/CD8⁺, triglycerides（TG）and total cholesterol（TC）and occurrence of adverse reactions were compared between the two groups after treatment.

The observation group detected a higher clinical efficacy rate compared to control group（P<0.05）. After treatment, the observation group had lower scores on TCM syndromes compared to control group（P<0.05）. The levels of BUN, Scr and 24 h urinary protein decreased, ALB increased and the improvement was more obvious in the observation group（P<0.05）. The levels of CD4⁺ and CD4⁺/CD8⁺ in both groups were increased than those before treatment, the levels of CD8⁺, TG and TC decreased, and the improvement was more obvious in the observation group（P<0.05）. No statistical difference was found in adverse reaction rate between two groups（P>0.05）.

Mahuang Lianqiao Chixiaodou Decoction has a high efficacy in treating PNS with damp-heat syndrome. It can improve the renal function, blood lipids and immune function of the patients and relieve the clinical symptoms while ensuring safety.

To investigate the effects and mechanisms of extracts from Baihuasheshecao（Hedyotis diffusa Willd）on the proliferation and apoptosis of renal cancer cells.

Thirty-two BALB/c mice inoculated with renal cancer Renca cells were randomly divided into model group, Baihuasheshecao（Hedyotis diffusa Willd）extract group, 5-fluorouracil（5-FU）group and transforming growth factor-β（TGF-β）/Smad pathway inhibitor（LY2157299）group, with 8 mice in each group. Corresponding doses were administered by gavage, and the model group was given an equal volume of physiological saline by gavage once a day for 14 days. The volume changes of transplanted tumor blocks in mice were recorded. The mice in each group were euthanized 24 hours after the last administration. The tumor mass was weighed, and the tumor inhibition rate was calculated. Western blot was used to detect the expressions of TGF-β1, p-Smad2, p-Smad3, Bcl-xl, Bad and cleaned-Caspase-3. Immunohistochemical method was used to detect the protein expressions of proliferating cell nuclear antigen Ki67 and PCNA. HE and TUNEL staining were performed on tumor tissues to detect the morphology and apoptosis of tumor cells.

Compared with the model group, the growth rate of transplanted tumors in all three experimental groups was inhibited, and the volume of transplanted tumors significantly decreased after 14 days of administration. Western blot results showed that compared with the model group, the three experimental groups up-regulated the expression levels of pro apoptotic proteins Bad and cleaned-Caspase-3, down-regulated the expressions of anti apoptotic protein Bcl-xl and pathway proteins TGF-β1, p-Smad2 and p-Smad3（P<0.05）. The immunohistochemical results showed that the expressions of Ki67 and PCNA in tumor tissues of the three experimental groups were significantly lower than those of the model group（P<0.05）. The results of HE staining and TUNEL staining showed that compared with those of the model group, the proliferation ability of renal cancer cells in the tumor tissues of the three experimental groups was inhibited, and the apoptosis rate was significantly increased（P<0.05）.

The extract of Baihuasheshecao（Hedyotis diffusa Willd）can inhibit the proliferation of renal cancer Renca cells and promote their apoptosis, which may be related to the inhibition of TGF-β/Smad signaling pathway activation.